LOCUS       DACDOA010000001        76044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDOA010000001 DACDOA010000000
VERSION     DACDOA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.001"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(70..1491)
                     /locus_tag="IWI39_000001"
     CDS             complement(70..1491)
                     /locus_tag="IWI39_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207143.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="IWI39_000002"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="IWI39_000002"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9207144.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /locus_tag="IWI39_000003"
     CDS             complement(3902..5158)
                     /locus_tag="IWI39_000003"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9207145.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            5738..7039
                     /locus_tag="IWI39_000004"
     CDS             5738..7039
                     /locus_tag="IWI39_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9207146.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7108..7707)
                     /locus_tag="IWI39_000005"
     CDS             complement(7108..7707)
                     /locus_tag="IWI39_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9207147.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7962..8843
                     /locus_tag="IWI39_000006"
     CDS             7962..8843
                     /locus_tag="IWI39_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207148.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8856..10466
                     /locus_tag="IWI39_000007"
     CDS             8856..10466
                     /locus_tag="IWI39_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9207149.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10543..11202)
                     /locus_tag="IWI39_000008"
     CDS             complement(10543..11202)
                     /locus_tag="IWI39_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9207150.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11485..12150)
                     /locus_tag="IWI39_000009"
     CDS             complement(11485..12150)
                     /locus_tag="IWI39_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207151.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="IWI39_000010"
     CDS             complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="IWI39_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9207152.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12930..13913)
                     /gene="dhaK"
                     /locus_tag="IWI39_000011"
     CDS             complement(12930..13913)
                     /gene="dhaK"
                     /locus_tag="IWI39_000011"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9207153.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="IWI39_000012"
     CDS             complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="IWI39_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9207154.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14464..14784
                     /locus_tag="IWI39_000013"
     CDS             14464..14784
                     /locus_tag="IWI39_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9207155.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14896..15765)
                     /locus_tag="IWI39_000014"
     CDS             complement(14896..15765)
                     /locus_tag="IWI39_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9207156.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15861..17165)
                     /locus_tag="IWI39_000015"
     CDS             complement(15861..17165)
                     /locus_tag="IWI39_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9207157.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17162..17671)
                     /locus_tag="IWI39_000016"
     CDS             complement(17162..17671)
                     /locus_tag="IWI39_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207158.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17846..19981)
                     /locus_tag="IWI39_000017"
     CDS             complement(17846..19981)
                     /locus_tag="IWI39_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9207159.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20064..21719)
                     /locus_tag="IWI39_000018"
     CDS             complement(20064..21719)
                     /locus_tag="IWI39_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9207160.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21721..22035)
                     /locus_tag="IWI39_000019"
     CDS             complement(21721..22035)
                     /locus_tag="IWI39_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9207161.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22205..22465
                     /locus_tag="IWI39_000020"
     CDS             22205..22465
                     /locus_tag="IWI39_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9207162.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22479..22793
                     /locus_tag="IWI39_000021"
     CDS             22479..22793
                     /locus_tag="IWI39_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9207163.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23523..24077)
                     /locus_tag="IWI39_000022"
     CDS             complement(23523..24077)
                     /locus_tag="IWI39_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9207164.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24203..26132)
                     /locus_tag="IWI39_000023"
                     /pseudo
     CDS             complement(24203..26132)
                     /locus_tag="IWI39_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26438..27154)
                     /locus_tag="IWI39_000024"
     CDS             complement(26438..27154)
                     /locus_tag="IWI39_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9207165.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="IWI39_000025"
     CDS             complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="IWI39_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9207166.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="IWI39_000026"
     CDS             complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="IWI39_000026"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9207167.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30624..32084)
                     /locus_tag="IWI39_000027"
     CDS             complement(30624..32084)
                     /locus_tag="IWI39_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9207168.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32074..33369)
                     /locus_tag="IWI39_000028"
     CDS             complement(32074..33369)
                     /locus_tag="IWI39_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9207169.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33701..34378)
                     /locus_tag="IWI39_000029"
     CDS             complement(33701..34378)
                     /locus_tag="IWI39_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9207170.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34408..35499)
                     /locus_tag="IWI39_000030"
     CDS             complement(34408..35499)
                     /locus_tag="IWI39_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9207171.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35600..36034)
                     /locus_tag="IWI39_000031"
     CDS             complement(35600..36034)
                     /locus_tag="IWI39_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9207172.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36618..38075)
                     /gene="ascB"
                     /locus_tag="IWI39_000032"
     CDS             complement(36618..38075)
                     /gene="ascB"
                     /locus_tag="IWI39_000032"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9207173.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38072..39967)
                     /locus_tag="IWI39_000033"
     CDS             complement(38072..39967)
                     /locus_tag="IWI39_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207174.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40025..41800)
                     /locus_tag="IWI39_000034"
     CDS             complement(40025..41800)
                     /locus_tag="IWI39_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9207175.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41850..43346)
                     /locus_tag="IWI39_000035"
     CDS             complement(41850..43346)
                     /locus_tag="IWI39_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9207176.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43540..45162)
                     /locus_tag="IWI39_000036"
     CDS             complement(43540..45162)
                     /locus_tag="IWI39_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9207177.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45490..46803)
                     /locus_tag="IWI39_000037"
     CDS             complement(45490..46803)
                     /locus_tag="IWI39_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9207178.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47048..47272)
                     /locus_tag="IWI39_000038"
     CDS             complement(47048..47272)
                     /locus_tag="IWI39_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207179.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47292..47660)
                     /locus_tag="IWI39_000039"
     CDS             complement(47292..47660)
                     /locus_tag="IWI39_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9207180.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(47856..48566)
                     /locus_tag="IWI39_000040"
     CDS             complement(47856..48566)
                     /locus_tag="IWI39_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207181.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48584..49081)
                     /locus_tag="IWI39_000041"
     CDS             complement(48584..49081)
                     /locus_tag="IWI39_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207182.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49118..49570)
                     /locus_tag="IWI39_000042"
     CDS             complement(49118..49570)
                     /locus_tag="IWI39_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9207183.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49705..50310)
                     /locus_tag="IWI39_000043"
     CDS             complement(49705..50310)
                     /locus_tag="IWI39_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9207184.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50760..51380)
                     /locus_tag="IWI39_000044"
     CDS             complement(50760..51380)
                     /locus_tag="IWI39_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9207185.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51382..52251)
                     /locus_tag="IWI39_000045"
     CDS             complement(51382..52251)
                     /locus_tag="IWI39_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9207186.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52274..52681)
                     /locus_tag="IWI39_000046"
     CDS             complement(52274..52681)
                     /locus_tag="IWI39_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9207187.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52700..53989)
                     /locus_tag="IWI39_000047"
     CDS             complement(52700..53989)
                     /locus_tag="IWI39_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9207188.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(54016..54318)
                     /locus_tag="IWI39_000048"
     CDS             complement(54016..54318)
                     /locus_tag="IWI39_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9207189.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(54343..56436)
                     /locus_tag="IWI39_000049"
     CDS             complement(54343..56436)
                     /locus_tag="IWI39_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9207190.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(56925..57689)
                     /locus_tag="IWI39_000050"
     CDS             complement(56925..57689)
                     /locus_tag="IWI39_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9207191.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="IWI39_000051"
     CDS             complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="IWI39_000051"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9207192.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(58694..59770)
                     /locus_tag="IWI39_000052"
     CDS             complement(58694..59770)
                     /locus_tag="IWI39_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9207193.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(59794..60387)
                     /locus_tag="IWI39_000053"
     CDS             complement(59794..60387)
                     /locus_tag="IWI39_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9207194.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="IWI39_000054"
     CDS             complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="IWI39_000054"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9207195.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(62057..62356)
                     /locus_tag="IWI39_000055"
     CDS             complement(62057..62356)
                     /locus_tag="IWI39_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9207196.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="IWI39_000056"
     CDS             complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="IWI39_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9207197.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(64111..65022)
                     /locus_tag="IWI39_000057"
     CDS             complement(64111..65022)
                     /locus_tag="IWI39_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9207198.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            65142..65999
                     /locus_tag="IWI39_000058"
     CDS             65142..65999
                     /locus_tag="IWI39_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9207199.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(66186..66548)
                     /locus_tag="IWI39_000059"
     CDS             complement(66186..66548)
                     /locus_tag="IWI39_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207200.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            66894..68333
                     /locus_tag="IWI39_000060"
     CDS             66894..68333
                     /locus_tag="IWI39_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9207201.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(68340..70892)
                     /locus_tag="IWI39_000061"
     CDS             complement(68340..70892)
                     /locus_tag="IWI39_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9207202.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(71006..72337)
                     /locus_tag="IWI39_000062"
     CDS             complement(71006..72337)
                     /locus_tag="IWI39_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207203.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(72303..73781)
                     /locus_tag="IWI39_000063"
     CDS             complement(72303..73781)
                     /locus_tag="IWI39_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207204.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="IWI39_000064"
     CDS             complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="IWI39_000064"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9207205.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        21722 a        16148 c        13042 g        25132 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
     5881 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
     5941 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
     6001 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
     6061 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
     6121 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
     6181 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
     6241 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
     6301 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
     6361 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
     6421 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
     6481 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
     6541 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
     6601 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
     6661 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
     6721 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
     6781 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
     6841 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
     6901 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
     6961 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
     7021 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
     7081 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
     7141 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
     7201 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
     7261 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
     7321 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
     7381 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
     7441 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
     7501 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
     7561 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
     7621 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
     7681 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
     7741 atatatatag ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa
     7801 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
     7861 taaaataata aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
     7921 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
     7981 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
     8041 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
     8101 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
     8161 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
     8221 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
     8281 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
     8341 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
     8401 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
     8461 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
     8521 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
     8581 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
     8641 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
     8701 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
     8761 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
     8821 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
     8881 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     8941 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     9001 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     9061 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     9121 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     9181 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     9241 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     9301 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     9361 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     9421 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     9481 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     9541 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     9601 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     9661 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     9721 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     9781 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     9841 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     9901 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     9961 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
    10021 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
    10081 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
    10141 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
    10201 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
    10261 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
    10321 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
    10381 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
    10441 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
    10501 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
    10561 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
    10621 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
    10681 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
    10741 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
    10801 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
    10861 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
    10921 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
    10981 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
    11041 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
    11101 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
    11161 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
    11221 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
    11281 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
    11341 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
    11401 tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt
    11461 ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag
    11521 tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg
    11581 atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc
    11641 ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc
    11701 tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat
    11761 cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg
    11821 gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc
    11881 gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt
    11941 cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt
    12001 ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc
    12061 aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa
    12121 caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat
    12181 tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag
    12241 tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa
    12301 ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg
    12361 tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag
    12421 actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat
    12481 gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct
    12541 cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca
    12601 atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga
    12661 ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg
    12721 attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg
    12781 ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc
    12841 tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc
    12901 atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct
    12961 aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata
    13021 tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc
    13081 aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt
    13141 ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta
    13201 acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca
    13261 ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct
    13321 acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc
    13381 ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg
    13441 attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt
    13501 tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct
    13561 aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa
    13621 actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa
    13681 gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg
    13741 gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc
    13801 ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat
    13861 ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc
    13921 ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat
    13981 accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata
    14041 acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct
    14101 accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac
    14161 gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac
    14221 ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact
    14281 atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct
    14341 aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg
    14401 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
    14461 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
    14521 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
    14581 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
    14641 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
    14701 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
    14761 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
    14821 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
    14881 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
    14941 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
    15001 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
    15061 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
    15121 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
    15181 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
    15241 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
    15301 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
    15361 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
    15421 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
    15481 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
    15541 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
    15601 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
    15661 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
    15721 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
    15781 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
    15841 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
    15901 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
    15961 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
    16021 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
    16081 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
    16141 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
    16201 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
    16261 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
    16321 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
    16381 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
    16441 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
    16501 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
    16561 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
    16621 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
    16681 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
    16741 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
    16801 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
    16861 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
    16921 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
    16981 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
    17041 acataacacc agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga
    17101 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
    17161 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
    17221 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
    17281 gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt
    17341 caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg
    17401 aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt
    17461 tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg
    17521 ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg
    17581 tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg
    17641 atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac
    17701 gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa
    17761 gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt
    17821 gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca
    17881 ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt
    17941 caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga
    18001 tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt
    18061 cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag
    18121 ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt
    18181 gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct
    18241 ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat
    18301 ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct
    18361 gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta
    18421 attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat
    18481 ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac
    18541 gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact
    18601 gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt
    18661 gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac
    18721 cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac
    18781 tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt
    18841 gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt
    18901 ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta
    18961 aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg
    19021 tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt
    19081 cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac
    19141 ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca
    19201 gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca
    19261 tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca
    19321 catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat
    19381 tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat
    19441 caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg
    19501 tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa
    19561 gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat
    19621 ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt
    19681 ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt
    19741 tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat
    19801 ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg
    19861 actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa
    19921 ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca
    19981 tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc
    20041 gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca
    20101 gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag
    20161 attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg
    20221 ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg
    20281 aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct
    20341 agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt
    20401 gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc
    20461 aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata
    20521 tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc
    20581 tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga
    20641 tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca
    20701 cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg
    20761 ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg
    20821 gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata
    20881 gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata
    20941 cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca
    21001 ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt
    21061 ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga
    21121 gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt
    21181 gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat
    21241 ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc
    21301 actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct
    21361 ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca
    21421 atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc
    21481 acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg
    21541 acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt
    21601 gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg
    21661 cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc
    21721 ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac
    21781 atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca
    21841 aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac
    21901 atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa
    21961 cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg
    22021 aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc atggggtata
    22081 ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt
    22141 ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg
    22201 tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat
    22261 tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt
    22321 aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa
    22381 aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc
    22441 gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg
    22501 atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc
    22561 cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg
    22621 ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc
    22681 gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg
    22741 tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag
    22801 aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact
    22861 tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca
    22921 gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa
    22981 gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga
    23041 gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
    23101 agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta
    23161 agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga
    23221 aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
    23281 ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga
    23341 aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat
    23401 cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga
    23461 gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt
    23521 ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa
    23581 agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa
    23641 agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc
    23701 gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct
    23761 tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac
    23821 aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct
    23881 gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca
    23941 acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc
    24001 agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag
    24061 tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag
    24121 cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat
    24181 attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct
    24241 gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt
    24301 cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg
    24361 ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt
    24421 ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt
    24481 tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat
    24541 tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg
    24601 ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat
    24661 gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga
    24721 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    24781 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    24841 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    24901 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    24961 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    25021 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    25081 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    25141 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    25201 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    25261 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    25321 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    25381 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    25441 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    25501 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    25561 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    25621 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    25681 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    25741 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    25801 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    25861 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    25921 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    25981 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    26041 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    26101 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    26161 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    26221 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    26281 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    26341 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    26401 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    26461 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    26521 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    26581 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    26641 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    26701 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    26761 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    26821 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    26881 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    26941 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    27001 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    27061 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    27121 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    27181 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    27241 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    27301 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    27361 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    27421 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    27481 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    27541 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    27601 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    27661 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    27721 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    27781 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    27841 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    27901 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    27961 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    28021 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    28081 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    28141 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    28201 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    28261 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    28321 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    28381 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    28441 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    28501 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    28561 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    28621 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    28681 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    28741 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    28801 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    28861 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    28921 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    28981 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    29041 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    29101 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    29161 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    29221 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    29281 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    29341 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    29401 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    29461 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    29521 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    29581 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    29641 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    29701 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    29761 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    29821 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    29881 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    29941 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    30001 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    30061 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    30121 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    30181 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    30241 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    30301 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    30361 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    30421 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    30481 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    30541 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    30601 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    30661 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    30721 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    30781 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    30841 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    30901 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    30961 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    31021 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    31081 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    31141 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    31201 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    31261 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    31321 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    31381 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    31441 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    31501 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    31561 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    31621 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    31681 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    31741 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    31801 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    31861 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    31921 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    31981 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    32041 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    32101 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    32161 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    32221 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    32281 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    32341 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    32401 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    32461 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    32521 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    32581 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    32641 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
    32701 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    32761 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    32821 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    32881 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    32941 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    33001 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    33061 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    33121 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    33181 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    33241 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    33301 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    33361 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    33421 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    33481 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    33541 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    33601 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    33661 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    33721 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    33781 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    33841 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    33901 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    33961 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    34021 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    34081 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    34141 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    34201 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    34261 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    34321 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    34381 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    34441 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    34501 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    34561 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    34621 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    34681 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    34741 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    34801 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    34861 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    34921 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    34981 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    35041 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    35101 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    35161 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    35221 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    35281 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    35341 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    35401 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    35461 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    35521 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    35581 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    35641 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    35701 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    35761 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    35821 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    35881 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    35941 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    36001 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    36061 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    36121 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    36181 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    36241 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    36301 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    36361 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    36421 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    36481 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    36541 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    36601 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    36661 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    36721 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    36781 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    36841 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    36901 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    36961 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    37021 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    37081 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    37141 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    37201 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    37261 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    37321 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    37381 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    37441 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    37501 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    37561 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    37621 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    37681 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    37741 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    37801 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    37861 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    37921 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    37981 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    38041 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    38101 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    38161 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    38221 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    38281 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    38341 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    38401 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    38461 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    38521 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    38581 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    38641 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    38701 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    38761 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    38821 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    38881 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    38941 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    39001 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    39061 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    39121 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    39181 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    39241 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    39301 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    39361 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    39421 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    39481 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    39541 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    39601 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    39661 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    39721 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    39781 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    39841 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    39901 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    39961 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    40021 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    40081 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    40141 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    40201 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    40261 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    40321 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    40381 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    40441 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    40501 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    40561 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    40621 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    40681 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    40741 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    40801 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    40861 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    40921 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    40981 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    41041 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    41101 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    41161 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    41221 aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa
    41281 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    41341 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    41401 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    41461 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    41521 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    41581 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    41641 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    41701 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    41761 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    41821 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    41881 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    41941 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    42001 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    42061 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    42121 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    42181 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    42241 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    42301 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    42361 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    42421 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    42481 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    42541 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    42601 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    42661 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    42721 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    42781 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    42841 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    42901 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    42961 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    43021 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    43081 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    43141 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    43201 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    43261 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    43321 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    43381 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    43441 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    43501 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    43561 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    43621 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    43681 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    43741 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    43801 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    43861 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    43921 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    43981 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    44041 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    44101 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    44161 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    44221 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    44281 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    44341 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    44401 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    44461 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    44521 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    44581 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    44641 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    44701 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    44761 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    44821 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    44881 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    44941 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    45001 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    45061 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    45121 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    45181 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    45241 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    45301 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    45361 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    45421 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    45481 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    45541 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    45601 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    45661 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    45721 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    45781 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    45841 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    45901 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    45961 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    46021 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    46081 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    46141 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    46201 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    46261 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    46321 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    46381 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    46441 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    46501 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    46561 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    46621 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    46681 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    46741 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    46801 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    46861 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    46921 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    46981 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    47041 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    47101 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    47161 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    47221 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    47281 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    47341 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    47401 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    47461 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    47521 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    47581 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    47641 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    47701 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    47761 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    47821 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    47881 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    47941 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    48001 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
    48061 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    48121 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    48181 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    48241 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    48301 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    48361 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    48421 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    48481 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    48541 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    48601 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    48661 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    48721 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    48781 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    48841 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    48901 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    48961 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    49021 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    49081 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    49141 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    49201 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    49261 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    49321 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    49381 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    49441 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    49501 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    49561 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
    49621 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    49681 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    49741 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    49801 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    49861 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    49921 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    49981 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    50041 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    50101 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    50161 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    50221 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    50281 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    50341 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    50401 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    50461 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    50521 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    50581 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    50641 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    50701 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    50761 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    50821 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    50881 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    50941 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    51001 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
    51061 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    51121 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    51181 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    51241 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    51301 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    51361 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    51421 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    51481 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    51541 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    51601 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    51661 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    51721 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    51781 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    51841 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    51901 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    51961 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    52021 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    52081 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    52141 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    52201 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    52261 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    52321 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    52381 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    52441 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    52501 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    52561 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    52621 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    52681 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    52741 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat
    52801 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    52861 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    52921 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    52981 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    53041 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    53101 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    53161 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    53221 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    53281 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    53341 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    53401 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    53461 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    53521 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    53581 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    53641 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    53701 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    53761 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    53821 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    53881 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    53941 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    54001 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    54061 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    54121 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    54181 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    54241 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    54301 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    54361 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    54421 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    54481 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    54541 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    54601 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    54661 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    54721 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    54781 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    54841 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    54901 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    54961 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    55021 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    55081 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    55141 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    55201 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    55261 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    55321 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    55381 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    55441 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    55501 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    55561 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    55621 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    55681 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    55741 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    55801 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    55861 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    55921 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    55981 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    56041 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    56101 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    56161 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    56221 agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg tacggatctc
    56281 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    56341 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    56401 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    56461 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    56521 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    56581 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    56641 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    56701 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    56761 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    56821 aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag aaaaaggagc
    56881 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    56941 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    57001 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    57061 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    57121 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    57181 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    57241 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    57301 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    57361 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    57421 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    57481 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    57541 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    57601 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    57661 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    57721 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    57781 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    57841 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    57901 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    57961 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    58021 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    58081 gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca agcctgatat
    58141 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    58201 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    58261 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    58321 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    58381 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    58441 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    58501 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    58561 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    58621 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    58681 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    58741 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    58801 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    58861 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    58921 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    58981 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    59041 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    59101 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    59161 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    59221 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    59281 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    59341 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    59401 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    59461 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    59521 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    59581 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    59641 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    59701 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    59761 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    59821 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    59881 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    59941 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    60001 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    60061 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    60121 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    60181 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    60241 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    60301 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    60361 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    60421 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    60481 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    60541 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    60601 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    60661 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    60721 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    60781 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    60841 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    60901 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    60961 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    61021 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    61081 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    61141 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    61201 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    61261 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    61321 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    61381 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    61441 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    61501 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    61561 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    61621 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    61681 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    61741 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    61801 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    61861 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    61921 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    61981 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    62041 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    62101 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    62161 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    62221 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    62281 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    62341 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    62401 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    62461 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    62521 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    62581 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    62641 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    62701 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    62761 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    62821 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    62881 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    62941 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    63001 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    63061 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    63121 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    63181 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    63241 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    63301 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    63361 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    63421 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    63481 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    63541 gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    63601 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    63661 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    63721 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    63781 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    63841 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    63901 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    63961 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    64021 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    64081 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    64141 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    64201 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    64261 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    64321 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    64381 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    64441 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    64501 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    64561 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    64621 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    64681 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    64741 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    64801 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    64861 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    64921 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    64981 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    65041 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    65101 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    65161 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    65221 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    65281 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    65341 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    65401 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    65461 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    65521 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    65581 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    65641 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    65701 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    65761 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    65821 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    65881 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    65941 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa
    66001 aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca
    66061 gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta
    66121 aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta
    66181 gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt
    66241 tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa
    66301 tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata
    66361 ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg
    66421 accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta
    66481 aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc
    66541 gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag
    66601 aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc
    66661 aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt
    66721 attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga
    66781 aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa
    66841 cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt
    66901 cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac
    66961 aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg
    67021 aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca
    67081 tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta acaaatgacc
    67141 tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta
    67201 caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga
    67261 aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga
    67321 cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat
    67381 tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc
    67441 attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga
    67501 ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag
    67561 attcttctat ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat
    67621 tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct
    67681 ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc
    67741 attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc
    67801 ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc
    67861 atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat
    67921 atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa
    67981 aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac
    68041 aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac
    68101 cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct
    68161 tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga
    68221 tttccaattt ccctctagat tctctagata tccctattgt atacatttct tctattccaa
    68281 cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc
    68341 tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct
    68401 ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt
    68461 gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc
    68521 ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct
    68581 tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag
    68641 acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat
    68701 aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca
    68761 gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac
    68821 tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    68881 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    68941 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    69001 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    69061 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    69121 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    69181 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    69241 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    69301 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    69361 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    69421 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    69481 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    69541 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    69601 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    69661 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    69721 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    69781 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    69841 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    69901 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    69961 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    70021 atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc
    70081 gaaagaagaa tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    70141 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    70201 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    70261 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    70321 cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    70381 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    70441 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    70501 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    70561 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    70621 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    70681 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    70741 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    70801 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    70861 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    70921 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    70981 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    71041 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    71101 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    71161 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    71221 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    71281 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    71341 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    71401 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    71461 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    71521 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    71581 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    71641 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    71701 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    71761 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    71821 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    71881 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    71941 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    72001 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    72061 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    72121 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    72181 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    72241 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    72301 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    72361 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    72421 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    72481 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    72541 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    72601 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    72661 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    72721 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    72781 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    72841 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    72901 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    72961 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    73021 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    73081 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    73141 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    73201 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    73261 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    73321 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    73381 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    73441 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    73501 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    73561 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    73621 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    73681 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    73741 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    73801 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    73861 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    73921 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    73981 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    74041 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    74101 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    74161 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    74221 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    74281 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    74341 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    74401 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    74461 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    74521 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    74581 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    74641 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    74701 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    74761 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    74821 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    74881 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    74941 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    75001 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    75061 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    75121 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    75181 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    75241 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    75301 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    75361 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    75421 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    75481 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    75541 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    75601 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    75661 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    75721 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    75781 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    75841 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    75901 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    75961 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga attttttttt
    76021 tataacatca aatccacgta gatt
//
LOCUS       DACDOA010000002        64604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDOA010000002 DACDOA010000000
VERSION     DACDOA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.002"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..641
                     /locus_tag="IWI39_000065"
     CDS             3..641
                     /locus_tag="IWI39_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9207206.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(760..832)
                     /locus_tag="IWI39_000066"
     tRNA            complement(760..832)
                     /locus_tag="IWI39_000066"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Thr,seq:cgt)
     gene            953..1195
                     /locus_tag="IWI39_000067"
     CDS             953..1195
                     /locus_tag="IWI39_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207207.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1334..1963)
                     /gene="upp"
                     /locus_tag="IWI39_000068"
     CDS             complement(1334..1963)
                     /gene="upp"
                     /locus_tag="IWI39_000068"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9207208.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2097..3044)
                     /locus_tag="IWI39_000069"
     CDS             complement(2097..3044)
                     /locus_tag="IWI39_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9207209.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3197..4441)
                     /locus_tag="IWI39_000070"
     CDS             complement(3197..4441)
                     /locus_tag="IWI39_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9207210.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4632..5657)
                     /locus_tag="IWI39_000071"
     CDS             complement(4632..5657)
                     /locus_tag="IWI39_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9207211.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5679..5858)
                     /locus_tag="IWI39_000072"
     CDS             complement(5679..5858)
                     /locus_tag="IWI39_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207212.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5851..6690)
                     /gene="prmC"
                     /locus_tag="IWI39_000073"
     CDS             complement(5851..6690)
                     /gene="prmC"
                     /locus_tag="IWI39_000073"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9207213.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6683..7756)
                     /gene="prfA"
                     /locus_tag="IWI39_000074"
     CDS             complement(6683..7756)
                     /gene="prfA"
                     /locus_tag="IWI39_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9207214.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7759..8343)
                     /locus_tag="IWI39_000075"
     CDS             complement(7759..8343)
                     /locus_tag="IWI39_000075"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9207215.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8449..8918)
                     /locus_tag="IWI39_000076"
                     /pseudo
     CDS             complement(8449..8918)
                     /locus_tag="IWI39_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9024..9626
                     /locus_tag="IWI39_000077"
     CDS             9024..9626
                     /locus_tag="IWI39_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9207216.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9730..10299)
                     /gene="lepB"
                     /locus_tag="IWI39_000078"
     CDS             complement(9730..10299)
                     /gene="lepB"
                     /locus_tag="IWI39_000078"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9207217.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10529..11296)
                     /locus_tag="IWI39_000079"
     CDS             complement(10529..11296)
                     /locus_tag="IWI39_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207218.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11293..11772)
                     /locus_tag="IWI39_000080"
     CDS             complement(11293..11772)
                     /locus_tag="IWI39_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9207219.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11897..12634)
                     /locus_tag="IWI39_000081"
     CDS             complement(11897..12634)
                     /locus_tag="IWI39_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9207220.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12795..14195
                     /locus_tag="IWI39_000082"
     CDS             12795..14195
                     /locus_tag="IWI39_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9207221.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14418..15770
                     /locus_tag="IWI39_000083"
     CDS             14418..15770
                     /locus_tag="IWI39_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9207222.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15763..16461
                     /locus_tag="IWI39_000084"
     CDS             15763..16461
                     /locus_tag="IWI39_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9207223.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16523..17674)
                     /locus_tag="IWI39_000085"
     CDS             complement(16523..17674)
                     /locus_tag="IWI39_000085"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9207224.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17687..18124)
                     /locus_tag="IWI39_000086"
     CDS             complement(17687..18124)
                     /locus_tag="IWI39_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9207225.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18139..19905)
                     /locus_tag="IWI39_000087"
     CDS             complement(18139..19905)
                     /locus_tag="IWI39_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9207226.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20169..20846
                     /locus_tag="IWI39_000088"
     CDS             20169..20846
                     /locus_tag="IWI39_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207227.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20849..21976
                     /locus_tag="IWI39_000089"
     CDS             20849..21976
                     /locus_tag="IWI39_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207228.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22176..22616)
                     /locus_tag="IWI39_000090"
     CDS             complement(22176..22616)
                     /locus_tag="IWI39_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9207229.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22627..24660)
                     /locus_tag="IWI39_000091"
     CDS             complement(22627..24660)
                     /locus_tag="IWI39_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9207230.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24662..26365)
                     /locus_tag="IWI39_000092"
     CDS             complement(24662..26365)
                     /locus_tag="IWI39_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207231.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26459..27409)
                     /gene="manA"
                     /locus_tag="IWI39_000093"
     CDS             complement(26459..27409)
                     /gene="manA"
                     /locus_tag="IWI39_000093"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9207232.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27448..28473)
                     /locus_tag="IWI39_000094"
     CDS             complement(27448..28473)
                     /locus_tag="IWI39_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9207233.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28682..29551)
                     /locus_tag="IWI39_000095"
     CDS             complement(28682..29551)
                     /locus_tag="IWI39_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9207234.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30255..32147)
                     /locus_tag="IWI39_000096"
     CDS             complement(30255..32147)
                     /locus_tag="IWI39_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207235.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32144..33871)
                     /locus_tag="IWI39_000097"
     CDS             complement(32144..33871)
                     /locus_tag="IWI39_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207236.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33887..34537)
                     /locus_tag="IWI39_000098"
     CDS             complement(33887..34537)
                     /locus_tag="IWI39_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9207237.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34741..35037
                     /locus_tag="IWI39_000099"
     CDS             34741..35037
                     /locus_tag="IWI39_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9207238.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35229..35849
                     /locus_tag="IWI39_000100"
     CDS             35229..35849
                     /locus_tag="IWI39_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9207239.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35941..37107)
                     /locus_tag="IWI39_000101"
     CDS             complement(35941..37107)
                     /locus_tag="IWI39_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9207240.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37119..37472)
                     /locus_tag="IWI39_000102"
     CDS             complement(37119..37472)
                     /locus_tag="IWI39_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9207241.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37600..38622
                     /locus_tag="IWI39_000103"
     CDS             37600..38622
                     /locus_tag="IWI39_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9207242.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39061..39495)
                     /locus_tag="IWI39_000104"
     CDS             complement(39061..39495)
                     /locus_tag="IWI39_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207243.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39605..41464)
                     /locus_tag="IWI39_000105"
     CDS             complement(39605..41464)
                     /locus_tag="IWI39_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9207244.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41582..42742)
                     /locus_tag="IWI39_000106"
     CDS             complement(41582..42742)
                     /locus_tag="IWI39_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9207245.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43049..44473)
                     /gene="araA"
                     /locus_tag="IWI39_000107"
     CDS             complement(43049..44473)
                     /gene="araA"
                     /locus_tag="IWI39_000107"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9207246.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44489..45187)
                     /locus_tag="IWI39_000108"
     CDS             complement(44489..45187)
                     /locus_tag="IWI39_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9207247.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45144..46805)
                     /locus_tag="IWI39_000109"
     CDS             complement(45144..46805)
                     /locus_tag="IWI39_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9207248.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47023..48423
                     /locus_tag="IWI39_000110"
     CDS             47023..48423
                     /locus_tag="IWI39_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9207249.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48675..49754)
                     /locus_tag="IWI39_000111"
     CDS             complement(48675..49754)
                     /locus_tag="IWI39_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9207250.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49840..50799)
                     /locus_tag="IWI39_000112"
     CDS             complement(49840..50799)
                     /locus_tag="IWI39_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9207251.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50811..52334)
                     /locus_tag="IWI39_000113"
     CDS             complement(50811..52334)
                     /locus_tag="IWI39_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9207252.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52344..52908)
                     /locus_tag="IWI39_000114"
                     /pseudo
     CDS             complement(52344..52908)
                     /locus_tag="IWI39_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(52914..53777)
                     /locus_tag="IWI39_000115"
     CDS             complement(52914..53777)
                     /locus_tag="IWI39_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207253.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53791..54687)
                     /locus_tag="IWI39_000116"
     CDS             complement(53791..54687)
                     /locus_tag="IWI39_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207254.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54862..56133)
                     /locus_tag="IWI39_000117"
     CDS             complement(54862..56133)
                     /locus_tag="IWI39_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207255.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56123..57640)
                     /locus_tag="IWI39_000118"
     CDS             complement(56123..57640)
                     /locus_tag="IWI39_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9207256.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57838..58728
                     /locus_tag="IWI39_000119"
     CDS             57838..58728
                     /locus_tag="IWI39_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9207257.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58801..59625)
                     /gene="nadE"
                     /locus_tag="IWI39_000120"
     CDS             complement(58801..59625)
                     /gene="nadE"
                     /locus_tag="IWI39_000120"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9207258.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59637..61109)
                     /locus_tag="IWI39_000121"
     CDS             complement(59637..61109)
                     /locus_tag="IWI39_000121"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9207259.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61746..62108)
                     /locus_tag="IWI39_000122"
     CDS             complement(61746..62108)
                     /locus_tag="IWI39_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9207260.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62331..63083
                     /locus_tag="IWI39_000123"
     CDS             62331..63083
                     /locus_tag="IWI39_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9207261.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63223..64146)
                     /locus_tag="IWI39_000124"
     CDS             complement(63223..64146)
                     /locus_tag="IWI39_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9207262.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64161..64433)
                     /locus_tag="IWI39_000125"
     CDS             complement(64161..64433)
                     /locus_tag="IWI39_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207263.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18612 a        13300 c        11267 g        21425 t
ORIGIN      
        1 tcatgaataa cattttagga aaaaaaatag aacttaaaaa agggatattg ggcactattg
       61 tcgttgtact cggtttgtta gggttgctat ttgcgccatc cattattagc attttttctt
      121 taactatcac tggatttata gcatttacac ttgtttttgg taaaaaagat acgaaaaaaa
      181 tgttctcgaa acctgtcgct cctgtcaaaa atatagccaa atattttttt atcaatgtag
      241 taatcagtgc cgcagtctcg gtatttttgc aacaaatttt gaaatggggt ttaacaggca
      301 atccaataaa tgaggctttc agcccgttat tatttattat tttgcctatc atgatacttg
      361 gagaagaatt gttctcagtg tattttctcg ctattttttc tagtaagttc agcattcctg
      421 tcgctagttt cttgtcagca atcatctttg gatttatcca ttacagtact tatgataatg
      481 gaaatatttt acacactgta gcacacatcc ttttgataca aggggtggca agattgttat
      541 tcaaccaagc tgccattaaa agcaactcga tcattacaag ttgggcagtt catgtgatat
      601 ttgatttcac agctatattg ctagtcgttt tattctccta aatttttata gaaataatac
      661 atgctctatt gctaagaaag catactttat ttcctttaaa caaaaagaca gatgtatcaa
      721 tggattaaaa atccttgata tatctgtctt ctcttttaat gccagctata gggatcgaac
      781 ctacgaccta cgcgttacga gtgcgttgct ctaccaactg agctaagctg gccttatcca
      841 aaagaaatta tatactctat ttggctgatt gtaaagagtc cgatggaaaa ttagtttaat
      901 ttcatgatat ttttcataaa acgtggtata attgatgtga aaggcaggtt ttatgaaaat
      961 ggcaactttt ggaaaatttg atacaactat cgtaccatcg gaaattggtg aaaaattcaa
     1021 gatcaatact cacgtaatgt tcacaattca cggcaagtcg caaactggaa cgatttcaaa
     1081 gcaattgaaa aattccgctg ttgttgagat tgatgaaact gcagaaaatg cgaacttgat
     1141 gacgcaaagt aagggtgtag taatcgttaa ctacaaagaa ctaaaaaaga tttgaatttg
     1201 accatctctg catttttggg atggtttttt tatatgcaaa cgaaagctgt gaaaaagctg
     1261 ttctgcatca aacgttaaaa atgaccaaaa aaggatgtga caaaagttgt gtcacaccct
     1321 ttttcgtatt tacttatttt gtaccaaaca aacggtcacc cgcatcccct agtccaggaa
     1381 cgatataacc atgttcattc aatttttcat ctagagaagc tgtatagatg tcaatatcag
     1441 gatgcgcttc ttgcaaagct tttactccct caggtgctgc taccaaacaa acaaatttaa
     1501 tgttcgacgc gccacgttct ttcaatgcat cgattgccat gattgctgat ccgcctgttg
     1561 ccaacattgg atctactaca aacaactgac gtgcatcaat gtcttctgga agtttgacga
     1621 aatattcatg tggttccaat gtttcttcat ctcgataaag accgatatgt cccactttag
     1681 cagctggaat caattccaag ataccgtcaa ccattccaat acctgcgcgc aagattggaa
     1741 taatcgctac ttttttacca gacaacgttt tttgtgttgt ttcagtgatc ggtgtttcga
     1801 tcacgatgtc ttccaatggc atatcccgtg atacttcata tgccatcaac atagcgattt
     1861 catcgactac ttcacgaaaa acctttgttc cgcagttttt gtcccgaata atagttagtt
     1921 tgtgttgaat caatggatga tcgatcactt gaaatttgcc cattttttaa gctctccttt
     1981 gttctgtcgt ttctttttaa ttgtaaaaca aaatcaagtg attgactagt caaactcgcc
     2041 atttttccta agaaaatccg tttttgaaaa gaagttttcc tgcggacaag caaagcttat
     2101 tcttttttct tgatccaaga agtagataat gccattgctg cacgcgttgc ctttgtctga
     2161 tccatagcat ttaccatgac aaaatcatgg atgattcctt ggaagcgtac ttgtgtaaca
     2221 ggaacccctg catcacgaag tttgcgcgca taagcctcac cttcatctct taatacatcc
     2281 gcttctcccg ttaaaatcat tgctgaagga agatcattta gttcatttat atttgctctc
     2341 aaaggcgaag ctgtgatttc agcacgctct tcttcattcg tcgtatattg gtcccaaaac
     2401 cacatcattc cttctcttgt caaaaaatag ttttctccga attcatgata ggaatctgta
     2461 tcaaaatcag cgtttgtgac tggataatac agcaattgct tggatattgg gaggcccttt
     2521 cgttcttttg ttaagatcgt catgactgta gccatgttac cacctactga atctcctgca
     2581 acgattagat tctcgagatc cagatttttt gacttggcta atttattcag ctgttgcaac
     2641 acagcataat tttgttcaat tgctgttgga tattttgctt ctggtgatcg actatattct
     2701 ggaaagacaa cgacggaatt cgtacgaacc gcaagttctc tgatcaattt atcatgggtt
     2761 tgagcactgc caaaaaccca gcctgctcca tgaatataaa aaataactgg taatttatcc
     2821 atattccctt ctggtcgtac gagacgaata ttgagttttc cccattcgcc agtatccacc
     2881 atcaagtctt caatatcgac ggaatatttg ttcacaggtg attcttgtac ttcttctagt
     2941 aaaactcttc cttcttccgg tgctagttca tagatacgcg gatgaggcgc atttgcatca
     3001 ctgaatgcta ctgcttcctt ttctaacgag atacgatcag tcatgacttt tcctccctga
     3061 gatttagtgt gaagactaaa attacacctc atatactaga gagcatcacc gatattgtca
     3121 aagaatccgt tttcccaaaa aataaacaga aaagcccaac attgaacttt tctgtttact
     3181 aatggaattt aaatttttat tttttatgca acggaaattt ttcagttaat tcgcgtacac
     3241 ctgttttgac ttcgtctagt tgagcgttat catcttttgc ttgtaatgct ttgacaacta
     3301 gttcagccac ttttacagca tcttcttctt tgaacccgcg agtagtgatt gctggcgtgc
     3361 cgattcgaat accgcttgtt ttgaatggac ttagtgtttc aaaaggaatc gagtttttat
     3421 ttacagtgat attcacactg tccaaaatgc tctctgcttc ttttccattt atacctaact
     3481 cgcgaacatc gatcaacatc agatgattat ctgtcgcccc tgaaatgact ctggttccaa
     3541 ttgcttgatt gaaaactttg accattgctt ttgcattggc gatgatttgt tcactatatt
     3601 ctttaaatgc aggatctaat gcttctttga atgctactgc tttacccgca atcacatgct
     3661 ctagagggcc gccttgaata ccaggaaata ccgcactatt tatttttttt gctaatgctt
     3721 catcgtttgt caaaatcatg ccgcctcgag ggccacgcaa tgttttatga gtggtagtcg
     3781 ttgtgatatc tgcataagga actggattag gatgaagtcc tgctgctact aatcctgcga
     3841 tatgtgccat atctaccatc agttttgcgc ctacttcatc tgcgatttct ctgaatttcg
     3901 caaagtcgat cgttcgtccg taagcactgg cacctgctac gatcaatttt ggttgatgtt
     3961 ttctagctaa aatacgtacc acattataat caataacttc tgttgtcgga tcaacaccgt
     4021 aagcaacaaa gtgataggtt ttgccactga agttgactgg tgatccatgt gttagatgtc
     4081 cgcctgcgga aagatccatt cctaagatcg tgtcccctgg ttctaccaaa gcgagataag
     4141 cagccgtgtt ggcttgagag ccggagtgag gctggacgtt cgcaaatttc gctccgaata
     4201 attcttttgc acggtcaatc gctaaatttt caacgatatc gacaaattca cacccaccat
     4261 aatagcgatg ccctggataa ccttctgcgt atttatttgt caagatactt ccttgtgcag
     4321 ccatcactgc ttcggagaca aagttttcag aggcaatcag ctccaaatta tgttcttgtc
     4381 tttcttcttc tttggcgatc gcagcccaaa gatcagggtc aaacgttttg taatctacca
     4441 caaacaacag ctccttttta ttttcctaca attttatttt cctacaattt tatttttccc
     4501 atcaaaaaca aattgccaaa tattcgtatc tcttctatta cgattagtta cgcttagttt
     4561 ttcgttcact gatagaagat ttacttgata gcattgtatc atactaaaag aatgcgacaa
     4621 taaccaattg atcatttttc aaaataattt tgtcctgctg cttttttcaa gcgattcatg
     4681 taagcattcc ccaatccagt ttctggatac acttgtacaa aaatcaaatc taatccaagt
     4741 gttggttcat ctaatcctct tagacctgca aacaatcctt tagccgccgc ttctactgag
     4801 ttgtcgttat acatataaac agctgcagta tctgtccgca cttgatccgc aatttcgggg
     4861 cttgctatga cacctgcacg gatcttctta ttttttgccc attgaactgc agttgaccaa
     4921 tcgccatctc tgaccatcag aacacgtgta tcgggtgagt agtgtttgta tttcatccca
     4981 ggggcttttg gtgtttcatt ttcttttaca agatgctgat caatagcaac aggagattcg
     5041 atgactgctt cgatttgttc tttcgttact gcacctggtc gtaaaatcat aggcatggct
     5101 gttggatcac ttaaatcaag aacagtggat tctacaccaa ttcgcgtagc tccatcatca
     5161 ataatcccgg taatttttcc ttgtagatca tgatatacat gatctgcagt cgttggactt
     5221 ggttttcccg acgtatttgc acttggaccg acaagcggaa caccggaaag ttcgattact
     5281 tctagtgttt ttttgttatc cggtatacga aatgcagctg tggataatcc tcccgtcaca
     5341 actgagggca aagtgtcttt tttgatctta aatatcaaag ttaatggtcc aggccagaag
     5401 ttcttcacaa tcttctctgc tgccggatga aagttgtcca catactcttt tacttgttca
     5461 aaagaggcga catggacgat taatggatta tcttgcggtc tcccttttac agaaaacact
     5521 ttcttgacag cgtttggtaa aagagcgttg gcgcccaatc cgtatactgt ttcggtagga
     5581 aaagcaacaa gttctccttc tttcagtgct tcggctgcct cttgcaattc tgtttcatca
     5641 tatcgtttcg tttccacagt ttatccacaa cccctttttc atacattctt attttgtctg
     5701 agtttatcct caagttttcc actgtttgtg gataatctat ttgtcaatag tggataaact
     5761 tgttgataaa ccaaaaagtc gtttacatcc ttgtcaaata agtgtttttt tataataaaa
     5821 aaacaaataa aaaatttttc atccacaact tcaaccataa acttggatca ttcgttcatt
     5881 gccaaaaaga tcctttttta tcgccacttt tttatctgga aatgcttgct gaaatatttt
     5941 ttttactgct tcaccttggc gaaatccgat ttctaaaaaa atctttccat ttgattttag
     6001 tacgcttggt gcttcaactg ctattttttc ataaatagct aatccatcgt tctcagcaaa
     6061 aagggccatt ttaggttcga atgaccggac actttcatcc atcagttccc attcattccg
     6121 actgatgtac ggcggattag aaataatcac atcaaattgc tgatccatta ctggtcctaa
     6181 tgtatctcca tgataaaaac tgattttcgt tccttctttc tgagcatttt ctttcgctac
     6241 ttctaatgct tcttctgaaa gatccacagc agatatatgc caattttttc ttgctgcttt
     6301 caaactaata gcaattgctc cagtccctgt cccaatatct atcacttcta acggtacacc
     6361 aggcgtctcg tctaaacacc attcgactaa ctcctctgtt tctggtcgag ggatcagtgt
     6421 tgcttcagtt acctttaacc gatgatcata aaaatctgca tatcctagca aatactgagg
     6481 tggatgatct gcaagcagaa gtcgcagatc ggtttcgatc aaccgctgtt cttcttctga
     6541 aatctcttcg ttcatatgaa ggagccaatc aagttttttc cattgttttc gttccaaaaa
     6601 taaaaattgg atactatatg cttctttccc atgatcctct aaaaaagaag aagcccgtga
     6661 caggacttct cgataggtca ttttacccat tttgcatagc ttccagtttt gatgtttgat
     6721 catacatgat caatgcatca acgatctcat ctaatttccc tgctaaaatc tgatctaatt
     6781 tttggatagt taatccgata cgatgatcag tcacacgatt ttgcgggaaa ttatacgtcc
     6841 ggatccgttc tgaacgatcc ccagttccga ctgctgattt tcgattggca tcatactcgc
     6901 tttgtgcttc ttgctggatc ttatcgtaca cacgagcacg caagattttc attgcttttt
     6961 cacggttctt gatttgagaa cgttcatctt gcatagcaac aacgatccct gttggtaagt
     7021 gagtcaaacg tacagcagat gctgttttat tgacgtgctg tccgccagca cctgaagcat
     7081 gatagatatc tgtacggata tctttgtctt caatatcgat ctctacttct tcagcttctg
     7141 gcatcacgac aactgttgcg gtggaagtat ggatacgccc ttgcgattca gttgaaggaa
     7201 cacgctggac gcggtgcgca ccactttcat attttaattt cgaatatacg ttgtctccgg
     7261 tgatcatcat gatcacttct ttatatccac cgattccggt aacgttagct tccatcactt
     7321 ctgttttcca gccttgtgct tcagcatact tttggtacat attgaaaaga tcgccagcga
     7381 ataatgctgc ttcgtctccg cctgctgctc cacggatttc catgatgata tttttatcgt
     7441 catttggatc ttttggtaaa agcaagatct tgattttttc ttctaagact tctttttctt
     7501 tttttaattc agataattct tcttttgcta gttcagccat ttcatcatcc agtttttcac
     7561 caagcagttc ttctgtatcg gtgattcctt gtactacttc tttataacga cgataaactt
     7621 cgactgtttc gcgcgtgttt gcttcttctt ttgaaagctg cataaaacgc tttgtatcag
     7681 aaatcacttc tgggtcactt aaaagttctc caagctcttc ataacggtct tcaattgctt
     7741 gtaattgatc gtacatgctt attctccttc tgtaatcatt gatgggtgaa agtaatgttt
     7801 tcgacaaacg gggtaataag cttcatttcc gccaatttgc acttgatcgc cttcataaac
     7861 agggtttcca tcaatataat gtaaattcat gattgctttt ttgtggcaga accaacagat
     7921 tgttttcatt tcttcgattt tgtctgcata aagcaataaa tattttgatc cttcaaataa
     7981 ctcgtttcga aaatcatttt tcaaaccaaa tgccataacc ggaatatcca attcgtccac
     8041 aattctcgta aaagcaatga catgtttctt ttttaagaat tgcgcttcat cgatcagcac
     8101 acaatagggt ttgctttcca ttttctgaat tacgtcaaat acgtctgttt cttcaaatat
     8161 cggcatcgca ttacgtttca gtccgattcg gcttgaaaca acgcctaaac catctcttgt
     8221 atcaataccg cttgtcatta agacaactgg cttattttgt tcttcataat tatgtgcgac
     8281 ttttaggatc tcgatcgtct tgccgctatt cattgcgcca tacttaaaaa atagttgtgc
     8341 catgctcttt ttcaccttcc agtttgttcc tatggtatta tacttgattt tcagcatttt
     8401 ttacagtgag aattttcatc aagcacagat tagtattaaa tctattgctc acttttgttc
     8461 caattttttg acaagaacta aacatgggtt ccattcatcc cataaatttg gaaaaacttc
     8521 caaccttgaa aatcctaatg attcgtagaa acaaatagtt tgatcatata tagagtagtg
     8581 cccctcgtct acagtcttca cttgcaaata tttgtaatgt tttgctgcgt aaccttctgc
     8641 ttctttaaaa agttttgttc ctaatccttg gtgatgatag tcttttttta ttcccataca
     8701 atagaataga tttcggcagt atcttcactt gtctgtgaaa gagtgataaa gcctaaatat
     8761 tcttcttttc ttttttcaca ccataaaggt agtttacttg attcctcgat atatttttcc
     8821 gttgattcag gaagcccaaa ccactcaggc aagtctagta atatttctcg gacacacttt
     8881 cctttttcct ctctatcttt tacaaacgat atcggcatat tctttctaga ttctcccttt
     8941 ttgaattgag tatagcatgc tttctttctt ttcacgtgct ataataagtt aaaatttcaa
     9001 agttttacgt aaggagctat ttgatgacaa aaaatatttt tgattccatc gctaaccatt
     9061 atgattcccc tgaccgtcaa gcattagcca agattattcg cgaagaacta actacttatc
     9121 taccgcgcga cagccaccaa aaagtcctgc tggattacgg aggcggaact gggcttgtta
     9181 gtttaccgct tgcggaaaga ttcaaagaac tgatcattgc cgatgcttca gaaactatgc
     9241 tgaaaatggc agaagaaaag atccaagcag cagatctgaa aaatgtccga acgatccacg
     9301 ctgatgcttc tgtcgaattt cctgctgtcc aagctaatct catccttcta tcacttgtac
     9361 ttttacacat tcctgatacg gagaatattc ttacaaaact ttatgaaatt ttagctcctg
     9421 gcggtcaatt gatcatcgta gactttgaca agaatgaaca aattagtcat ccgaaagtcc
     9481 ataatggctt tagccaagaa gaattgaata atcgattgga aaaaacgggc tttgtctctg
     9541 ctactagtca taccttccat cgtggggaaa agctatttat gaacaaaaac gcttcgcttt
     9601 tcttatccat cagccaaaaa gcttaaaatg agattgcaaa aaaagtgagc aactccaaga
     9661 aataagaagg aattttcgga aaaagaagct gtgacaaaaa acatgtcaca gcttcttttt
     9721 attttttcat cacatgattt taatttctga aagaggccaa taacggaaaa caacttttcc
     9781 ttggatcata tctttgtcga ctaacccaaa cattcggctg tcttttgaaa tcaagcggtt
     9841 atcgcctaga acaagatact ttccttttgg cacagtatat tcaaagtcac tcgtaaaggt
     9901 ggatgaactt tctgcttgtg cttggaatcc tgagctataa gcatattctc cttgtaactt
     9961 atcttctgaa aattcttttt tgaactcatc cagataaggt tcatcatact ttttgccatt
    10021 aatggtcaac tggtcatttt ccatcttgac tgtatctcca ggcataccga tgatccgttt
    10081 aacaatcatc cgttcttcat cccccggttc ctttgtagta ataatatcca ttcgttcata
    10141 gtttgagatc ttagatgaaa tcaatcgttg tccatcactt aatgtagggt ccatcgaatg
    10201 cccgtttaca gtaactggtg taaacacgta cactctagct aataaaatag ccgctaatac
    10261 aaggatcaaa atcccccaat ttttcaaaaa ttctttcaca gcttacctcc tacttgtatt
    10321 ttcctcgtta tttattacgt tttttcacaa tacttcgttg tactttcttt attctacaat
    10381 gtttataaat gaaaggaaag aataagtgat gtgttcgtct tatctttctc gttatataaa
    10441 aaaggaacgt gacaaaagac gtgtcacatc ccccttattc cgaataaacg tttttatcgc
    10501 aaccactgat acacggcgtt tcatcacatt atgaatgtct tcttctttgg ctacgactat
    10561 aaaccactgg ttcttcctgc gactgtgcct tttttgcaat caatttcaga cgataaggct
    10621 catgatagcg taacccgtct ttgacaaaat caccgccgac gatgaacaat ccgacaggtt
    10681 ccaatgtgtc ttctgttgag tcaagtcgaa tggtccctac tttttccatg tccgagtagc
    10741 ttcgtccaat atttagaaca tactctttct ctcctgtttt cttgacatag taataaggat
    10801 acaatgtatt tctatcgacc agaaatccat ccatgcgacc gagttcattt gttttgatct
    10861 caccatttcc aatcaaatac cgcatattct cggctaattt caattgataa tcttcgtcat
    10921 aagaaaccat gacctcttgt gtattcactt tatcgatcga atttccatta tctatcgatt
    10981 cttctgtctg gatgttgaac gcttcatcag gtaaatactt cacaaaaagc tgagtgacat
    11041 cggctttcgt gtttttatct aaaaagtaat aaaatacatt taaaacagta aaataaacaa
    11101 gcactagaaa tgcaagtcca tataagaaaa attgaagata gttttggttt tgccaaagtc
    11161 cataaacaat tctcaataaa taaagcgtgg gaatcacact taaaatcgta tatccacggt
    11221 taagatattt aggactgatg tctaaatagc caaggaattt atgcaccccg ttgataaggt
    11281 ctagtaaaaa tgtcattgag aatcacctgc ttgctggtta tacgtttggg tatttcctga
    11341 atcttggttt tgctgtgctg tacgtgctgc atcatcattt tgcgtgttat catctacagt
    11401 cgtactgctt ggtatcgttt cggagctagc acttgtatcc ggacttgtac ttggattggt
    11461 tgaatccgat ggagtgccat ctggtaccgt attacttgtt tcgcttgagc tgtcagttgt
    11521 atatgtctca tcatacgacg aactagttgt tgtatcgtcc catgtacttg tcgtagtggt
    11581 ttcattcaaa ctttccgttg ttgttggttc agatgtcgag actgaagtag ttggtgttgt
    11641 ttcttctaca gtactagaac ttgaacttgg tgcacggtag ctggaactat tcacaatacc
    11701 tgtagtagtc gcgacaatcg tcgagattgt taatacagcg attggcttca aaataggtgc
    11761 tacttttcgc atcttatctc ttccttcatt gaaatttatt cttgtttcct tactttacta
    11821 cggacaaatt atacagtcta ccttatttct accataaatt ctttattttt ttcttacgtt
    11881 tttccgaaag atttctttaa ttttcctcaa aaactacggt tgatgttttt aagtaagctt
    11941 ttgcttcccg attgatttct ttcagatcgc atccttgctg cttggcagcg aacacgcaac
    12001 cacaataaca ttggcgataa atatcatact ctttgcacat ttgcaaagag cgttcatagc
    12061 ctttgttttt cttgaaatca cttggcaagt attgtgtccc gtaaatcttt tggatatcca
    12121 ttccgatctg attgatcaaa gctgcatttt tctttggcga gatggtcagg gcacttccaa
    12181 agtaatcgta tcctagttct tgcgcttttt gcgcaacaag atctaatcgc atattgaagc
    12241 atgcactgca gcgagcacca ccttcttttt cgttttcaag atggtttgtt aaaaccattt
    12301 tggtgaattc atttggacgg tattcatcaa cgattagcgc aacatgatgg tcagttttct
    12361 cattaaattg gtctacaaat cgtttttgtt catgtaatct tcgcaaatac tcacttttag
    12421 gatgaatatt cgaatttgaa aaaaataacg tgatctctgc atattgagac atgaattcca
    12481 atgtgtgcgt actgcaagga gcacagcatg tatgtattaa tatcttaggc tttacttctt
    12541 ctttctccca ttgcatgatc atttttttca gaactgtatc ataatttatt ttttgatttt
    12601 tcattttttc tacgatttgt tgagcattaa tcaatttgtt attcctcctc cttctttttt
    12661 ctaggatttt ccttattata agccatttct attttaatcg aaagctgtat tgttatctgt
    12721 ttgataattt ctttgagtat tcctaattcc ccttgtatac tggtagaaac gactattgtc
    12781 aggaggatag tactgtgcat gttgatttat tctttcgaag gctgcagcgg cgaggacaga
    12841 agtttgctgc caatcatttc tcatcgatcc aaatcatcgt tttttattat atattgatga
    12901 cggtcttatc actcgttcta ttttatatgc cgatttttcg cgaaccagat agccatgttt
    12961 cttttgtgga catgctgttc atggcaatca gtacagtgag tgtgactgga ctatcgactt
    13021 ttgatatcaa ttctgtgttc aatgataacg gtattatact gctggaaata ttatttcaag
    13081 ttggtggttt ggggatcatg atgatttcta ctgcttttgt cattttttct aaacgacgga
    13141 tcacactgaa acagcgacag ctgatcatga cggatatgaa tcaaccgcgt ttgtctggta
    13201 tcgtccgact gattcggatc acttttgcta ttttaatctg gtttcaatta ctttttggga
    13261 cattcttttc tatttatttt tattacagag gctattttga tcgttggcgt gatgccgttt
    13321 tttacggttt ttatcaggcg atatcggctg tcacgaactc tgggtttgac gtcactgggg
    13381 attccatcaa gccctttgca catgactatt ttttcttgat cctgatcatg tttttgattt
    13441 ttgtcggagg aattggtttt cctgtattga tggagtgccg ggaatggcta ttatacaaac
    13501 gttccaatgc gaagcttccg tttcgttttt ccttgtttac caaattagct gtgttagctt
    13561 ttgttatctt gtttgtttcg ggaactgtct tgatttatct attggaaaag gaccatttat
    13621 tccaagattc caatatgagt gtcaaatgga tcaattcgat gttttattcg attacgactc
    13681 gtaatgctgg tctgcagatt cacgatttgg gtgattttca aatcacaaca ctgatcattt
    13741 tttctttact gatgtttatc ggatgcagtc ctagttctgt aggcggcggg attcgaacaa
    13801 ctactgtagc aatcatcggt ttgtatctat attccttcct caaaagtgaa gataacatca
    13861 atatttttgg acggagaatc gatcaagatg atgtgcgaaa atcagtcgtt gtctttatgc
    13921 tttctcttgg tatgtgtttc ttttgtatcg tttttttatc tgctacagaa gaacaaacgc
    13981 tgatctcgat catcatcgaa gtaacttctg cgtttggaac aactggttta tctttaggga
    14041 ttaccggaga cttgtctgtt gtgggcaaaa tcacgattgc tacactgatg tttattgggc
    14101 ggatcggaat gctatataca ttgatgttat tcgtgccaaa agaaacacga gatctaggct
    14161 atgaatatcc aagcgagaaa attattatcg gttaaaatgg gaatataggt aagagggaat
    14221 aataaaatca cagcagcttg aaacaatcat tttgtttcaa gctgtttttt tgttaaatca
    14281 tgcgattttg tccgatttca tgcactttca gccgtaacct tttcttaaga ataagaaaag
    14341 caatttaacc tttttttcaa catatggtat gattagttga atttattcgt cagcaaatcg
    14401 tttttggagg ttacataatg ggaatacgca gtcattttgc gattgcagtt ggaaaaacgt
    14461 cgcaatgggc attaaaaact ttttttaaag gcggatcaag tttaccaggt aaactagcgc
    14521 tgaaagtcga tccacatata ctagatagtt tggcaaaaga ctaccaagtc gtcgttgtca
    14581 ccggaacaaa cggtaaaaca ttgactaccg ctttaacagt caatattttg cgccagcaat
    14641 tcgatgaagt tctaaccaac cctactggtg cgaatatggt acagggaatt gtatcgactt
    14701 tcttgcaggc aaaaccaaaa aaaggacaga aaaagtttgc tgtcctagaa atagatgaag
    14761 caagtttgag caaagtcaca gaatatatga aaccagaact gttcttattt acaaatattt
    14821 tccgtgatca aatggatcgg tatggagaaa tctatacgac ctataaaatg attgtagacg
    14881 gtgccgcaaa gtcaccaaat gcaacgatta tcagtaacgg tgattcacca atcttcaatt
    14941 ctatcgaaac agtcaatcct agaaaatatt atggctttga ccatgaagaa gatcgtgaac
    15001 aaatggctca ttacaataca gacggtgtct tatgtccaaa ttgtcatcac atcttgcatt
    15061 ataaaatgat cacttatgca aacttaggga aatattactg tccaaattgt gacttcaaac
    15121 gtcctgaatt ggattatcga ttaactgata tggtacggat ggacaatgtg tcagcagatt
    15181 tcgtgatcga tggtcatgaa tacggaatcg aagttggcgg tatgtataat gtctacaatg
    15241 ctttagcagc tactgcagtg gcagagtact tcaatgtcga tcctgataaa atcaaacaag
    15301 gtttaggata tgacgagaaa gtattcggcc gacaagaagt catcaatatc aatgggaaaa
    15361 aatgtacatt agtacttgtc aaaaatcctg taggtatcaa tcaagtaatc gatatgattg
    15421 gtctagctcc ttatccattt tctttagtgg ctttattaaa tgccaattac gcagacggaa
    15481 tcgatgtcag ctggatttgg gatggtaatt ttgaaaactt tgccgatatg gatatcccag
    15541 ctgttatttc tggaggagac cgtcatacag atatgacttt acgtctaaaa gttgccggga
    15601 ttccagaaaa taaattgaca gaaacaaaag aattaccaga agtaatcgaa aaaatcaaag
    15661 aattgccaac tgagcatgtc tatattttag ctacttacac tgctgtattg cagcttcgta
    15721 aagaactagc tgctcaagga tttatcaaag gaggaatgaa ccgtggctaa ttatgaactc
    15781 aatatcgccc acctttatgg aaatttgatg aacacatatg gtgataacgg aaaccttttg
    15841 atgttgaaat atatagcaga aaaaatgggg gtttcttgcc atacggagat cgttagtata
    15901 catgaacctt ttgatgctga taaatatgac ttggttttct ttggcggtgg tcaagacttt
    15961 gagcaattaa tcatttcaaa agacatccag aaaaagaaag attctttgat caactatatc
    16021 gaaaatgatg gcgtcatgtt agccatttgt ggtggctacc aattgctagg tcactactac
    16081 atgggagcta atggagaaaa gatccaagga atcagtgcac tggatcacta cacgttaagt
    16141 caggaaaata accggtttat cggtgatatc gttatccata acgaagaatt caatgaaact
    16201 tattatggat ttgaaaacca caatggtcgg actttcttag gtgaaggtga acgacctttg
    16261 gggaaagtgg tacaaggtca aggaaataat ggtgaagacc aatctgaagg tgtcatttac
    16321 cgaaatgtct tcggttctta tttccatgga cctatccttg caagaaatga gaacctggca
    16381 gctcgtttga tccgcctagc tttagaacaa cgttacggta aagaattgga tctgccctat
    16441 cttgaaggac aaacagccta gttattcgaa aaaggggaat gtgacaaaaa tttcgtcaca
    16501 ttcccctttt tcgtatccta ttttaagcga cttgcttggc ataacggttc acgttttgct
    16561 tgatattccc gattgttttc ggatcattga ttcctgtgac ttcttgaatc aattgttcca
    16621 agttttcatg gatcttcatt tcatgcaatt gacggctttg ttcatcttca ggatcatagt
    16681 aattaaaaat aatccccatt gttgccgtca aatgtgggca agtcaagtta tgttcttgta
    16741 gttctctcac tggacgtgta aatctttctt gataacctaa cttacgtaat ggtgtccgag
    16801 cgacacgtgt gatcgcatca gagatatttt tgttttggaa gcgatgaatg attttttcaa
    16861 tatacgcatg atgctgctct gcatcaaacc cccatttggc aatcagcaaa cttcctgttt
    16921 cctgcaaaac tgattttaat tgtgccacaa ctaacgcatc ttgcattgct tcatcaatgg
    16981 tctggtaacc taacaaggca cctgtataag cgacagtcgc atgaccagta ttgacactaa
    17041 atagtttgcg ttcgatatat ggttctagat catctacata taaaacccct tttaattgga
    17101 tttttttatt ttttctttgg ctatcgtcaa tgacccattc tttgaaaggt tccacttgga
    17161 cgaataaagg atcttcatgg tgttgtaatg ggacgattcg atcgactgct gcatttggaa
    17221 agccgatata tttgtcagta aatgatgttt ccggaagata tttatacact tctttttcta
    17281 gaaatgctga accgccaatc atattttcac aggcaatgat atctaaagat gttgtgcttc
    17341 cttccttttc tctttcttgg ataccttttg caatcaactc agcaataaaa ggtaagatat
    17401 tcgggccaat ggcggtagta actaaatctg cttcagcgat ttcttggatt actttttctg
    17461 gatttgtttg attgttgatt cctttgacat tttttacagt gattttttct tttgattcat
    17521 ctgctaattc gatttcgtat tcttttcttt catttaaagc atcaatgatc gtgtgattca
    17581 catctacgaa agtaatctca aatccattag ctgccaaaac ctctccaata aatcctcgac
    17641 cgatatttcc tgcaccaaaa tgtactgctc tcatttttct ctctccttaa gcaattgcta
    17701 gattttccgt tacttcttct ttgctgagcg cgtctgctag ctggacaaca ttgtccatat
    17761 cactgcaata aagtgcgatt tgctgtacaa gttgcaaatg ctcatctccg actcctgcaa
    17821 taccgaacaa aacagtcgca atcttttctt cttcttcggt accaaaatct acaccatctg
    17881 gtacttgaac gacacagatg ccggattttt tcacatattt ttttgcttca tctgttccat
    17941 gaggaatcgc gataaaattc cccatataaa cagataacat tttgtctcta tctaccatgg
    18001 catcgatata ttcctcatca acgcaccctg cttctacaag catctgaccg caatagcgga
    18061 tcgcttcttc tttggtttta aaagatcgat tgagctcgat catgtcgaat ggcaaacttt
    18121 gcatgctgtt ttcctccctt aagctgtctg tttcaaatat tgtgcaatcg attcgtattt
    18181 ttgcggattg accatgctgt tgactgataa atgaatggat tgaggtgcag ccatctgtac
    18241 tttttccgct aaactttctt tggctacgat cagtgaatca ggttcatctt ttaaatcttg
    18301 gatattgact tttcttaacg ggatagttaa atggttcttg tctaatatat ctttgagcac
    18361 ggtcatcccc attgttgctg atccggaacg atcatcgtgg acaagtagaa tctctttgat
    18421 ttgtttgatg tttgctgaaa gaaactcttc tgctggctca ttctcaattt cagaaactga
    18481 tgttttctgc ttgctgctta attcttgaat gatttcatca tatcgaggtg aattcaaaaa
    18541 gttttctact gagacaaaac gcgcgctcgg tgctttttgt tttgctcttt cttgtagttc
    18601 gacctgagta acgatcaatg tattcgagtc atctgtcagt tggctgattg cttgattggt
    18661 caccgtttga ggcaagtctg cttcctgtac tttttttctc aagatcgaag cgcccatcgc
    18721 acttgatccc attcctgcat cacaagcaaa aatgattttt tgtacgccag agaatgtagc
    18781 tccttgagga ataccttggg cacctttgga agcttgctta gctgcctttg tctcttgaac
    18841 agatttttcg aagtcttcat ttgtatcttt cgcatccgct ttcaaaatta ctgcagaaat
    18901 accaaacgaa acagctgcgg caacaatcac gcccaaaata acaggaaggt aagaattgaa
    18961 cggtgtcatc gctagaatcg caatgatcga ccctggagaa gctggcgctc ttagtccagc
    19021 atccatcaat tggaaaatga aacttcctga cattcctccg cctataactg ataagaataa
    19081 taacggtttc atcataacgt aaggaaaata gatttcatgg atcccgccta aaaagtgaat
    19141 gatcatggct cctggtgctg aagattttgc tgctcctttg ccaaacaaag taaaagcaag
    19201 taatacgcca agtcctggac caggatttgc ttcaagcaag aatagaatag attttccagc
    19261 ctctgctact tgttcagttc ccaatggtgt caaaatccca tgattgattg cattattcaa
    19321 aaataagatt tttgctggtt caataaaaat acttgtcaat ggaatcaaat gggcagataa
    19381 aatgatctct acaccttttg ccattgcttg agtcaatgca tcgacaactg gaccaatcgc
    19441 agaaaaacct aataacgcta atccaaaacc gataagacct gctgaaaaat tattgactag
    19501 catttcaaaa ccggatttga tccgtttttg gaatagctga tcaaatttct tgatggcata
    19561 tccaccaaga ggtcccatga tcatcgcgcc tagcatcatt ggaatatctg ttccgacaat
    19621 cacacccatc gttgcaattg ccccaacaac tgatccgcgg tcaccagcaa ttacttttcc
    19681 gccagtataa ccgatcaata aaggaatcag ataagttagc attgggctta ccatggtagc
    19741 gaaagcctca tttggtaaat aaccatctgg gataaataga gccgttaata caccccaggc
    19801 aatcagtgct ccaatatttg gcatgaccat gcttgataaa gtactgccta atttttgtac
    19861 cctcgctttt agcgaaatgt tgtttttggg aatcgtccgt tccattttgt ctctccctct
    19921 ctccttttga tactctaatt gtatccgctt actaaaaaga gaaatagtcg tactttggca
    19981 cgaaactttg tgccaaaggt ttgagtatgt cagcaaaata aaaagatttc ggtatcagaa
    20041 aacttcaatt ttaaataaaa ctgttatttt aattttttta taatatgcaa acaataactt
    20101 ttgttagcat ttaatttaga tgttttccat tctattatgt tattaaattt tatattggag
    20161 gcctatctat gacattactg atcaaaaaca tgaacatgaa acgtggtaaa aaacgtgtga
    20221 ttcgacaatt gaatctttta gtagaatcag gagaagtagc tgctttgatt gccccgaacg
    20281 gatttggcaa aacaactttg ctgaatggca ttgcccaact gctgccaaca acttatgatc
    20341 aattgtccct tcacaacatc tctcctactg cttatacaga ttatcgccgg tctttctttt
    20401 ttctagaatc aacgaatcag ttatccgact atctatcggc tgttgattat ttgactttga
    20461 tcaaatacta ttggcagagc aaagtagaat gccaaactgt tttccaacga ttgaagatga
    20521 ctgagtacct tactttgccg attggaaaaa tgtctctagg aatgaaacaa catgtcttgc
    20581 ttgctgctta cttgatcagt gattcatctg tcatgttatt cgatgaaccg ttgaacggat
    20641 tagacccagg aagtattgat atcttgaata gcctcatcag acaatggcag aaagagggga
    20701 agacgattct tctttcttcc cacgctatcg cgaatatcca atcgatttgt tctaaagcat
    20761 gttttctaaa aaatggagaa atcgtcacag aatcctcaaa tatggaagag attttagcta
    20821 tttatcgtaa attatatgag gtgtaactat gtctttactt acatttgaac gcaatctctt
    20881 actaaaaaac aaagtaaact ttttatttcc tctattgttg gtcgtgctct ttgcattccc
    20941 actttttttt gatcataaac ttgcttatac agaattcgat gaactgaatc ataactatga
    21001 agagatgcaa cgtttgattg aaacgttgaa agaagatgag aatgagaaag aatttgtgga
    21061 aagtttagag aaaagcaaca aactgatcga agcaatctta catgcaaaaa acacagggaa
    21121 tgtccagcaa atggtcgaag caacttatca ttatgaaaaa gatatactgg atcgactgat
    21181 ttctggtcaa agacaaggaa ttccaatcat tgaacaacaa aaacgtgtcg agttgctccg
    21241 ttatatgaaa gaacatcaga tccaaagata ttctattttt gacttgccag cgcatctctc
    21301 tttagcaaat tactacgaaa atattttttc aggaatgatt tctagttttt tgattttatg
    21361 tataaccgcc ttgtttcttt cttccatcat ctcttatgaa aaaagaaagc aggtaatctc
    21421 tctagtcaat ctattactgg atagtatggt aaagaaacat agcatccgtt ttaccattta
    21481 ttatggtgca gctatgctaa gtctcgtgat gccatttttg atcgtctcta tactagtaat
    21541 aatcaaaaat ggtttgggag acttccgtta tcctgtagga acgatcattg gtcaggagat
    21601 acgaatcctg ccgatgtatg agtatctttt ccagagcttt ctgttcctgt tgttgtgggt
    21661 gctcttttta agtacaatca gttttcttct atctgcttta tttgaacatt ctctagtcaa
    21721 tcttttaggt acacttcttt gtcttttctt agcagagtat cgcttgtttt cttcgattgg
    21781 atggatagaa tcaatcagtc attacttgcc aacttcttat gttgattttc aaaatgtgat
    21841 cataggagga gatattttct cgccactagc ttcagaacag gttactttca tgaatggtat
    21901 cttgacactt ggtatatgga gtatcgtttt attattcata ggtatgggca ctatctatat
    21961 aaaaaagagc tattaaaaca caaaaataat aggggcggtg acaaaaacct tgtcaccgtc
    22021 ccctatttca aataaacggt tttctagaat aaatagtttt caaaagcttg catccgcagt
    22081 aataagaacc tcccaacaaa aatagctcct cttattttta tcttggttga agttattact
    22141 tgatgcaagg cagctttttt tactgtgaac attggttata tatgtccatg ttcaaaaatc
    22201 atttggactt cttcaatggt ttccgcatca ctgagcgcca ttacttggtc gatgtctgaa
    22261 cagaaaaagg ctaaatcttg gagcaccgct aactgatgtt ctttttttaa agccactaca
    22321 aataagagtg tcgcgatttt tggttcctct tttgttccaa agtccacacc atcaggcact
    22381 tggactaaat agattccttc ttctactact tcttcatctt tgtcagtccc atgtggcaag
    22441 gctacgaaat tcccgatata gacactcgct ttttcttgtc tttcgatcat tgattggata
    22501 tatgccggtt tgattccccc ttgttgggtc atccactggc cgatctcggt caacgcttct
    22561 ttccaatttt gataggattg atcgagcaat attttttctg ctggtagctg tatcatcttt
    22621 tcaccttcat ttctttattt cttcaatcag gattttcgct aacaactgat aaacgatttc
    22681 tttatttcct gagcggaaga tctcggtgtt caagtcattc atgatgattg ctccactgat
    22741 ctttcctaaa agaatcccgt ctttttcttg gattggcgaa ggcgccagca taactaacat
    22801 acgggacaaa tcgactggct gtctatccat cccttcaatg gtcaacggat gtttcaaatt
    22861 gaaaatacaa aagatcggtt tagaaatcaa tgagctggat gtatggaata accccatatt
    22921 tgtatgagga ataccgatag gtgcttgttc atatcgctta agcagccggc gatgaaccac
    22981 ctcttcatcc gttactagtc cttcaggaaa ataaggaaat aagctgcgaa tcgtttcgga
    23041 aatcgtctca ggattatcca gttcttggat aaaaaaacga ccgagtaatt gattgatttg
    23101 atccatgaaa tgcattgttt cctcataagt atcttctatc atccactcgt tattgctgtt
    23161 atacgtaatt ttttgccttt ttttcttctc aatatgcgaa aaagcttctt ttagctgctt
    23221 gacttcatct tctagcagta gcggggagac taaaatatat tttccaccgt atcctggtaa
    23281 aatagatgta gaaataacaa catcatattc ttcttgtaaa tcgatttttt ttaaatcagc
    23341 aatccgatag aaatgaattt gttggatgaa gggaaaatac cttttcaatc gtagttccaa
    23401 catgctagta gaagctaatc cacttggcga aaatcctgca atactgatat tgctttcttt
    23461 cggacttcgt tccaatgaat tcgcaaaatg aaggaccata taagcgatct cttcttcgga
    23521 taattttttt tcttgaaaaa tccgctctac tgctttacgg attgcattcg caacttcctc
    23581 gtattgaacc atgatttttt ctaaaatcgg attggtcagt gtctcttctt gtaaaacggc
    23641 tcttgaaaac acaccagata aatgagtcaa cagcattttg tacaaagttt catcttcata
    23701 aaaatggaca ttcgtctctt cacttacttc ttgaatcagt tgttttacta aataagcaag
    23761 ctgtgtatcg aacgtctctt caaagaaatt ctgatcaaat gagttcgtga agtcatttaa
    23821 taatcctgca aaaaagacga tctcatttac tggataaagt tgttttgtct cagaagcgat
    23881 ccgtgaaaac aatcgtttag ctgtctgtag taattctttg tttgccagac tattatatgt
    23941 ctcatttgtc agtaaatgat ttttcgtcac acgctccatc gtcaatgtca gcatcaaaac
    24001 caaatgttca agctttctat cacttagatt ggtctttttt tggatcaatt cttccccgat
    24061 tactttgcgt gcaagagata gatgttgcgg atcaacgaat cgaaaaaaat aattcagtga
    24121 tgcatcgaaa gaaatactat gaaaaagttc atattcattg atttctaatt ctaatacatt
    24181 cgctgtcaga agccggcggt atttttccgg tccttcgatc tcgtatccct ggttccgaat
    24241 gatttccaaa ggcagttggc gaatactttc ttccaactgt ttgatatctg cataaaaagt
    24301 ggtattactg atgcgatatg attccatgaa atgagcaaga ctcaacggct ctgtcgtcag
    24361 taacaactcg gctaaaatcg cgtgttgtcg tttagcagtg gataagtcat tacttttccc
    24421 atctgtattt tcttttaatt gctctaatac ttcttctgat gcctcaatga caaatgagcc
    24481 tctagctgct tttttcaaag aagcatttac cgatttcaat gagacttcaa gattatctaa
    24541 ttcgcggtaa atagtccgtt tactgacctt caataaattc atcatttttt ggatagatac
    24601 aggtgacggg ctactgagaa attctgctaa taattgtttt tctctgggcg ataaatacat
    24661 cttatatttc tcccttcacg agtttttcgt attcattcgt agctacccaa tgttcaacta
    24721 gaactagccg agggatcttt tcttctagct gttcagttac agctagttct ctttgtgcga
    24781 tataaatcgt ttcttcttgg attttgattt tttctattga ttcttttgag ataacaaact
    24841 gttttttcgc tttttttgct aattgactta aaatcgtcat gcccatagtt tgtgagccgc
    24901 gtctgttgtt tttatacaaa aatacaatat tagcaggcga cgttttttgt tctgtgatat
    24961 ctgctatctc ttttttttct tcccgctttg gaaattggat cgcttctttt attcgtgaca
    25021 gcaagatatc tgtttccaaa aagtgatcca gtacatagat ttgactttga ggtacttttt
    25081 ctcttgcaag acccgctagt tcatgttgca cgataaccag ctgatttact tggttattca
    25141 attcatagat agagctgtat ccgacagaaa gatcgatttt ttctgctgct aatttctttt
    25201 tcagcagggc ggctcccata gcacttgatc ccatacctga gtcgcaagca aaaatgacgg
    25261 aatgaatgat agatggttcc aagatttctc ccctttcttt gaccgttttg ttctcttttt
    25321 ctttatttgg taatttcaat agtatcgctg cacaaatgaa ggtcacaaat gtactgattg
    25381 ctatccctat acttacccca gctagttgtg gataaggcgc attggcaaaa atcacgagta
    25441 gtgacccagg cgatacaggt gcagctaatc ccgtatctaa ccattgaaat atcaacgtgc
    25501 cgcttgcccc accagccatt acagctaaaa atagatacgg tcgcagcaag acaaatggaa
    25561 aatagatttc gtgaatcccg ccaatcgcat ggatcatcaa tgctccactc gcatttactt
    25621 tttcctgttt ttctgcaaaa caaaggatag aaagtaagat ccctaaacca ggtccaggat
    25681 ttgcctccat caaaaataag gaagagtacc cgactgtttg tgagaattcg attcctaaag
    25741 gtgtcaatac accatgattg attgcattat tgaaaaatag tacttttaga ggttctaaga
    25801 tcagactgac aagtggtaac agattcattg tgactagcca agacacaacg gaggcgagca
    25861 acaagtgaac actatttaaa actggttcaa taaaaacgat tccaattata caaataacgc
    25921 taccagtcag tcctactagt aggttgctga caagcatttc atatccagcc ttgaatttca
    25981 catggaatct agaaacaaat tgttcatata cgatgccggc aagcggcccg ataatcatcg
    26041 ctcctacgat ttgtggagca ttggaacgag cgatcaaccc taaagaagca attgcaccgg
    26101 cgactgcccc tttttgtccc cctaccgatt tcccgcctga ataactgatc aagatgggta
    26161 aaagcagctg gatcatccaa tggcatacct cttctaatgt ggtgtgcaaa tcgccagaca
    26221 caccttttgt tgctaatgac aagattcccc aagcgataaa cagacttaga tttggcatga
    26281 tcatttcatt taagtgatgt ccagcttttc tcagttgttt catagacacg cgactgctgt
    26341 tgtttgcttt ccatttgtat tccatctttt tcaccagact tctatttatt gatggacgga
    26401 ttttatgtag aaaaagcagc aaacgaaaaa ccgtctgctg ctttcagcta tctgggaact
    26461 atttgcctgg ttcagacgca atgattgcta gttcaccttg gaccgcccac tcagaaacgc
    26521 catttggaat aatacagctt gttccttttt ctaatggata tgtttttccg tcaacgacaa
    26581 ggtctcctgc tccttcgatt accgtcatca atgtataagg ggcttgtttt ttgaaagatg
    26641 tgattccttt aatatcccac tcatatacat tgaaaaactc agtttctaaa taagtgacga
    26701 tcgatgaatt cccttttcgg acttctttga tttgtaattc tggtgttttc gcagggacag
    26761 ttgttacatc gatcgattgt tggatatgca gctctcttgt ttgtccttgc gcatcttttc
    26821 tgtcgtagtc atacacgcgg taagtcgtgt cagaactttg ctgtgtttct aaaatcatga
    26881 ttcctttacc aatagcatgg atcgttccgc ttggtacata aaagaaatca ccttttttaa
    26941 ctggtacttt cttcaaaagg tcatcccagc gcccatcttt gatcatctca gctagttctt
    27001 ctcttgtctt tgcatgatga ccatagatga tttctgcacc aggttcagca tcgatgatat
    27061 accagcattc agtttttcct aattcacctt catgtttcat cccataggca tcatctggat
    27121 gtacttgtac agatagatca tcttctgcgt ctaagatctt gatcaataat ggaaacactg
    27181 gttctgaagg atggccaaac aattccttgt gttctgacca tagttcatct aatcttttcc
    27241 ctttgaattc gccattttct actacactga caccatgagg atgcgcactg atcgcccaat
    27301 cttctccgat tttatcgtta gggatatcaa aaccaaagac tgaacgtaaa cgacttcctc
    27361 cccaaatttt ttcttgaaaa actggtttta aaaacatagg ttcctgcata attcaaactc
    27421 ctctcatttt tcatagctat tatagcatta tttcggtaat accgctttct ctttcaaaaa
    27481 gttttcaaca atttcatctc gacatatgag atactccgca cgatgatcga caggatgtac
    27541 gcgatttgac aaaaatatca acgcttcttg tgtttttata tcaatcaaga aaaacgtacc
    27601 tgtataacca gtatgaaaaa gaatcgcatg accgtcttct acatcctttt ttaagtccca
    27661 tcctaaagaa cgatagccat tttttttcgg tgtttgatct tgcagtaaag aatcaatcgt
    27721 ctcttcttct aaaaaacgac cattacggat ttttcccttg ttcaaataca tttggacaaa
    27781 agtaatgctg tctttcaaat caataaagag acccgcgtta cctgcatgtt cagccaaaac
    27841 tcttgctttc ggatcatgag tttgtccacg caaaacctca cccgaaggca gttgttctgt
    27901 gggaacgatt tttttggaag gaaaaggaag aaataagctg ttcatcattc ctaatggttc
    27961 caatacttgt tcttgaaaga tttccactgc agtttcttgg taaatttcct ctagcatcaa
    28021 cccaagaaga atcgtaccgg catctgtata ttttacttct tttcctagtt gctcacctgg
    28081 ttctagcttc aaataagcgg cgatcaattc ttcctgagat agcttatctc tgtttggtat
    28141 ccatgttttg atatcagaag tatgtgtcag taaatgacgc aaagtgattg tctgattttc
    28201 aaattcagga agatatttat gaagcggttg atcgagttct atctttccat cttctaatag
    28261 acgtaaaact aatgaagtgg ttgcaacgac tttcgtcagt gaggctgcat caaacaacag
    28321 atcttctgtc atcagctgct tttcaggaac gacttgggca aagccggctg taaatgtttc
    28381 tacttgattt tcttttatga aggcgactgc tacacctgga taaaatcctt cttccatctt
    28441 tttcaaaata agctgtttcg tgtgtggata catctttttc tcctgctttt ctatcagatt
    28501 cttacagtcc aagcttaaag cttaatctat gaggaatcaa ccttttttac acaaaaaaac
    28561 tgcgcgaaaa accgcgcagt ttcccccggt atactatttt ctctttctat ctctagaata
    28621 agcgatagta tgaccgttca taaaatgacg agacatgcga caatcgttaa gaaagaaaaa
    28681 gttaagcgtc gcatcgaatg tcagttttat tcttttcttt tggctcactg acgaaccacc
    28741 attcgattcc tatagcatca tagattgtga gtactgtgtg ctttttgtct aagaagaaat
    28801 caactgatag ttgctgcaag tgagcaatca gtccttccat gctttcagta tcgatcatga
    28861 atttcagaaa atcaagtcca atcacttcat ctgtaggcaa atcaattgcg cctccgctag
    28921 cttctgtcaa accaacatgg aaatctcctt cattcaatac atggatgccc tcttcttcat
    28981 tatgtacctt caaaccaagt acatgattaa gaaattggcg ttcttttgtt aagttagtaa
    29041 catttaaatg gattttttca aaataagaac ctgttgatag tttactgaat ttttcttcag
    29101 attttcttaa taatgcttct tgatccattg tttctggaat ttctctttgg tcgttcttat
    29161 tctcttcatg gtaaatttcc aattcattct cttctgggtc tacgagcaaa atccccatac
    29221 gtccatgatc ttctaaagct tctttgattg gataattcat tttacggata cgacaaagga
    29281 tatctgccat ttcttctgtg ctagggataa tcaatgaaaa acggttcagc tttttgatca
    29341 cccccatgtg atcattcgct ctaggacttt cttccagcag caacatttca cttccagctt
    29401 cttctgttcc taaaatggct aattcgtttt cttctctttt caaatcaaag ccaacaacgt
    29461 tcttgtaaaa atcaatcatt ttatctcggt ctttcacccg aatggctaca ctttttaaat
    29521 aggtggcagc ccccaattga aaatctttca tatttcttgc tcccctctac gtcaagcttt
    29581 attttaacga tggattcttc ttctttcaag caataacttt gattttgtta aaaaaaatgt
    29641 gagaaaattc gcattttgct tgttttttgt aaattaatga gtaaaaactc ttataaaaag
    29701 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
    29761 cctatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    29821 gaagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
    29881 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
    29941 accaagaggt tgtgagcctg ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca
    30001 gcttctcagg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
    30061 ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac aaaacggaaa
    30121 aatagcttct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
    30181 cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa aaaaaataat
    30241 aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata aagatctgca
    30301 tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat cgaaccatga
    30361 ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa
    30421 cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc tgttcgtgta
    30481 tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa gaatgcccgc
    30541 gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc gtttaataca
    30601 gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc
    30661 tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc atagatactt
    30721 cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt
    30781 gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac atcgtagaac
    30841 cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac aatagcgacc
    30901 atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc tggtgtgtag
    30961 ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc tggaaggcca
    31021 gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc ggctgaagca
    31081 actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg agagaactgt
    31141 gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt tgcaactttt
    31201 actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt catcaacggc
    31261 atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt ctgattttct
    31321 ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat ggtatgacca
    31381 ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg atctgcgaaa
    31441 aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg gatcatcaat
    31501 aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac cccaatcaat
    31561 gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc
    31621 atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata ataggaaatc
    31681 ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt tcgttgggaa
    31741 acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg
    31801 attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt tgttacttgt
    31861 tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc tgtggttgct
    31921 ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc tagtacaaat
    31981 acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg taccgttccc
    32041 cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc accatgtcct
    32101 ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct aagtcctcct
    32161 ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat agttcttcat
    32221 gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct gcatccatta
    32281 ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc gttttcaatg
    32341 cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca tcaaacacat
    32401 agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt tgtccaccag
    32461 agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc tctgaaacaa
    32521 aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt gcatccggct
    32581 taccatactg catattgtct cggatcgttc cagagaacag aactgctttt tgaggaacaa
    32641 agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt ccatttattt
    32701 ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag gtcgttttac
    32761 ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg aaatcaatat
    32821 cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg aaggccaatg
    32881 ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg ataccgattt
    32941 tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc accatgataa
    33001 aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc ataaatgcaa
    33061 tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac caagtgattg
    33121 ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg acgatcgtat
    33181 taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga ttttcttcaa
    33241 agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct cgaaatacca
    33301 ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca aagtacatga
    33361 ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt gtcaactgat
    33421 gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca ttgatcatca
    33481 tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc gttcttgtga
    33541 tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct gattttttat
    33601 agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca aaaaatgtat
    33661 ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc
    33721 aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag gtaagtgtcg
    33781 gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca attgctgaag
    33841 tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat ttatatactc
    33901 cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct actgagtaat
    33961 cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca gtattcatca
    34021 ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc gtccgatcga
    34081 tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt
    34141 tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg ttcataaaca
    34201 agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat
    34261 aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt aacatatctt
    34321 tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct ttatcttcaa
    34381 aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta gcaatcgaag
    34441 cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt cttttttgtt
    34501 tttctttagg caaatggaaa aatgtctgtg tcggcatatg gattctcctt tctttcaaga
    34561 tgacacattg tcatatgacg ctatgtcatt ataacacctc ctacctgttt tgcaagacat
    34621 ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag gaatccttcc
    34681 atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag gatgagataa
    34741 atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc tgacagcatc
    34801 gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca ctcgctttct
    34861 atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa actttattat
    34921 attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac tgctaatggt
    34981 tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt tgcttgaata
    35041 ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct cttttatacc
    35101 ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata gccttttctt
    35161 tttcgagtaa actgattttg ttagtcatag ttataacaat catagcaata aaaggagaag
    35221 gtaagcatat ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc
    35281 gcacagttaa ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg
    35341 aagtgactgt tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat
    35401 caggttggga agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac
    35461 aaaaaattgc tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta
    35521 tcgcaaacgc cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg
    35581 tcaatgtagc tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag
    35641 gtaaaaaagc actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct
    35701 ctcaatatgt aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca
    35761 tcgaaggaat cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata
    35821 aagctgctgc tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag
    35881 aggaaaaaat cttcaaagca gcatttgtgc cttggatatt ttcccatctg tctctttttg
    35941 ttattctttc tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca
    36001 ttcaaaaaca tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac
    36061 accgatgatt ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg
    36121 gattgctaac tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc
    36181 tactgcaaaa acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa
    36241 gatagccgga atcatcgcta aaaatgatcc taagtaaggg atcaggttta aaaaacccgc
    36301 aataatcccc aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat
    36361 gattgccaca gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt
    36421 catttcactc agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa
    36481 ataaggtgct aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag
    36541 cagagccact aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact
    36601 cttagatata tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc
    36661 caactgttcg cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc
    36721 aatcgtatct acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt
    36781 agggatgatc actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat
    36841 gatactgtaa agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa
    36901 gtaatacaag attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat
    36961 tggtgcaaac aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac
    37021 aacaagtaag gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg
    37081 tctgttcttc ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt
    37141 cccaaaccaa tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct
    37201 tcttctggac aagcatcaaa aatcaatgat acgtgagcta ctgtctttaa attcccattg
    37261 gccatcggac cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct
    37321 gctttcaaag aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag
    37381 gcagctgttg ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct
    37441 gtcccaattt ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa
    37501 aacagtataa cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa
    37561 cgaaaactat ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta
    37621 ggaacctata cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa
    37681 gaagaattag ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa
    37741 agtaaagctg gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg
    37801 tacgataagg attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat
    37861 gtagcggttg atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta
    37921 aatgtctacc atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa
    37981 gaagcgacag gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact
    38041 cctgaccaac gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg
    38101 tccgaaaacg gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct
    38161 cgccatcttg tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc
    38221 acagtccgcg tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata
    38281 tcgaccttgc cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat
    38341 gatggcaagt tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt
    38401 tttgaagatg gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc
    38461 cgcgatttcg cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat
    38521 aatctgacat tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat
    38581 gtctatgtac cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa
    38641 atgaatcagc aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat
    38701 gggaaaatcg aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta
    38761 cagaactact ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc
    38821 tgaaaagaac atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa
    38881 aaaccgccga gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta
    38941 caaggttgtt gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat
    39001 aaaggtaata ttacatttat attagataac tctagttttt attgttatca aagatttctt
    39061 ttattttttc aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc
    39121 ttggtcttgt tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc
    39181 gatactttgg atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt
    39241 tttatgaatg cccattgctt ttgctggtgg acgcaaagta ctgactttca taagcattgc
    39301 ccgatatttt tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc
    39361 cagttcactt gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat
    39421 tccttctccg attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc
    39481 aatattcggt aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact
    39541 tgtttttatc ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc
    39601 attcttattc gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt
    39661 catattggat caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt
    39721 ctttatacca ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt
    39781 ctaatgataa aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat
    39841 tcgctgcaaa ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc
    39901 ctttactgtc gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt
    39961 ggatcgtatc tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc
    40021 tagaaagatt cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat
    40081 cttgcagata attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt
    40141 ctagattatc atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga
    40201 actgaggtgt tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag
    40261 aaatatatgg attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact
    40321 ggtcaaatcc taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat
    40381 ggatcgctgt cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat
    40441 ccatttttgg tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag
    40501 aaaaaccagt caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa
    40561 gcatattgcc actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag
    40621 atacttctac gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga
    40681 tgttcggcga ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt
    40741 cagtgatttc tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct
    40801 tcagattgta caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt
    40861 aaggtatcca tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga
    40921 aattactgat ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt
    40981 ggaatcgcct tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg
    41041 ttcctgcggc agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc
    41101 cgatttcagt gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt
    41161 tcatataatt tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag
    41221 ggacagatcc tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta
    41281 agaagaattt ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat
    41341 cattttgaca ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga
    41401 ttgctgctgt tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt
    41461 tcaaatgctt gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg
    41521 tacgaaattc gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc
    41581 tttatggcct ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta
    41641 gctgttgttt cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt
    41701 agacatgctc attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca
    41761 gcagagaaaa tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa
    41821 acggcgcttg gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga
    41881 acaaaagata atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct
    41941 gagtcgatcc ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt
    42001 ctggaaaatc agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat
    42061 atttatcaac aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt
    42121 ctatattcgc ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga
    42181 tgtaatccga ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg
    42241 catcgcggat agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt
    42301 ttgtgcctat cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca
    42361 cataagcact tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta
    42421 ccatacgact tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct
    42481 cttttactgc attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag
    42541 ctttttcaac gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg
    42601 gtgtaagcca ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat
    42661 catctattat tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat
    42721 caatcaaatg aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct
    42781 gcaattagac aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga
    42841 gtaggctgag tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag
    42901 agcaaaatat tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa
    42961 taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa
    43021 aactaagtgg ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta
    43081 attttgccca cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac
    43141 cgccacctgc ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata
    43201 acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat
    43261 tgatcagcag tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg
    43321 ctttcccatc aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa
    43381 caacgattct tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca
    43441 agattgcttc ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt
    43501 tccctcgagc catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc
    43561 cagcaaaacc gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca
    43621 ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac
    43681 ggcgaatacc gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt
    43741 cataatcacc atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt
    43801 cttcgtctga tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag
    43861 tccaaccaaa ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca
    43921 tgttgtcacc aaaacgagcc acttttattc caaaactttc attataggct actgcaacat
    43981 ccatccaatc agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt
    44041 tattttgttt atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt
    44101 gatttaaatt catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg
    44161 tagctaaatg aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg
    44221 gtgagaaagt atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt
    44281 tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag
    44341 gcaattggcc attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc
    44401 gcagtgcttc ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc
    44461 caatattcaa cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc
    44521 cgtgttttcg taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat
    44581 tgagctgtct ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta
    44641 ccgcactttg cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg
    44701 gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt
    44761 catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc
    44821 cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc
    44881 agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat
    44941 ataaaacagc atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat
    45001 tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag
    45061 gtttgataac gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt
    45121 tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt
    45181 ccagcatttt ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg
    45241 atcccctcac ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt
    45301 gtgatggtga ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac
    45361 ttcccatgtc gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct
    45421 ccggctgtgt ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca
    45481 acttctggtg ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca
    45541 gatttcaata tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc
    45601 atgaaattcg ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca
    45661 tttactcctg taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc
    45721 gtatcgcctt ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc
    45781 atacccaaag aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag
    45841 cagttattcg tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt
    45901 tccggatgga cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca
    45961 gccgaaatgt tccctgtccc tttggctaca ctgttcgtag cgaccatacc ggttcctgca
    46021 tccccttctg gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga
    46081 taagcccctg attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa
    46141 atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc
    46201 atttcttgat ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct
    46261 acgcctagta ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa
    46321 aaattaattt ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata
    46381 ctccatctct gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca
    46441 gtgattgtat ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct
    46501 agataaccgt gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca
    46561 tagatttcga atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata
    46621 tcttctaatg aataggtcca gataccattt tctagtttgt tttcccactc ataactacca
    46681 gcggcaatag gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac
    46741 tcaatcccta aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc
    46801 ttcataatga tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc
    46861 gcttacaatg attattgtat acttttgtac gtacatgtca agcagttatt tacaagaata
    46921 caataaaaaa cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc
    46981 tgatcaaccg ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa
    47041 gtttcctcat cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat
    47101 attggtgtta tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa
    47161 gcttctttga taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct
    47221 ttatctggtc ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt
    47281 atttttatgg ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg
    47341 attgcagcaa ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct
    47401 tatatgtctg aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg
    47461 atgattgctt ctggcatgct cctttcctat gttgcagatt atttgctaaa agggttgcct
    47521 gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt
    47581 ttcggtgttt tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa
    47641 gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa
    47701 caaatccagc tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt
    47761 ttagaaaaat atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt
    47821 caaggagcta atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat
    47881 gcagcgagcg atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct
    47941 ttgctctttt tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt
    48001 agcgtgatgg gactttcttt catcctacca gctgttttag gaactgtact ggatgctcat
    48061 acaaattcgt tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca
    48121 tgggctccac tgacatgggt aatcgtaggc gaaatattcc cactagctgt ccgcgggcga
    48181 gcatctggcc tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt
    48241 cctgtgatga ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc
    48301 tttttagggg ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag
    48361 gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct
    48421 taaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc
    48481 agtcacaagg aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg
    48541 accagttggt aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga
    48601 gcgatgacaa aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg
    48661 taaaaacccg tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt
    48721 tcatagagaa tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg
    48781 gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa
    48841 tggcttaaag aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca
    48901 ttttctgtca acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca
    48961 tctaaagctt gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt
    49021 tccgtggtga aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg
    49081 ctgataaaac ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac
    49141 agacgctgat gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca
    49201 tctaaacaaa tataagaggc atttaactct tcataagcag cattgatcat caccatcgga
    49261 atgccttgtt ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc
    49321 gtaggctcaa cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc
    49381 tccaattcgt gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat
    49441 tcattttcga ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg
    49501 acaccgatcg tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca
    49561 tatgtgcccg accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct
    49621 tgccgaatcg tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg
    49681 gttgtattta cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct
    49741 tgatattttg ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc
    49801 tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg
    49861 ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg
    49921 attaggattg tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa
    49981 aatatcaaag cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt
    50041 atttttttca gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa
    50101 aagattcttg tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact
    50161 accagaatac gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac
    50221 tgaaaaaccc cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt
    50281 taacgtccaa ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg
    50341 atcttgttgg gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa
    50401 actgaaagtt tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg
    50461 atctatatgc tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt
    50521 tttatgattg cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg
    50581 tgcccagatc aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc
    50641 agcatgttct aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg
    50701 aaccgatcct gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg
    50761 ttgccgaacg ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc
    50821 tttattctaa ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt
    50881 tctattgaaa aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg
    50941 tccaacagat cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca
    51001 tgttcaatcg ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga
    51061 ttgccgttat ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca
    51121 taaacagggc aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt
    51181 tgcatcacat aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa
    51241 ggtattgtgt ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa
    51301 atagccagcc ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg
    51361 atgggactac cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat
    51421 tcatcaaacg ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa
    51481 gtagcaacga tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct
    51541 gtatcatcac gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata
    51601 tagttatgca aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc
    51661 cattcagggt acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa
    51721 tcatccacta acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca
    51781 ctcttagccc ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg
    51841 ccaaaaggct ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga
    51901 aaattacaat attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc
    51961 gtgaatatcg gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa
    52021 gaatttggtt ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta
    52081 cctacagtat ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca
    52141 cctaattcta attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct
    52201 gtttcatggc ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat
    52261 gagccaaaca gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc
    52321 acttcttcgt ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca
    52381 agtttccata cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt
    52441 acaaatccta ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc
    52501 catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat
    52561 atccaggata aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt
    52621 acgattgtct gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga
    52681 ctaaggaaca agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata
    52741 tttcctgatc cctcaccttt ccattgcgcc attacataaa aaaacagcac atgtagcagg
    52801 cacgatagta ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc
    52861 agaaaccaca atcaactgtc cataaagttt agtctcaact attttcatct tcctcactct
    52921 ttcattccag acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact
    52981 agtaatggca gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc
    53041 tgccctgtaa ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat
    53101 atcaatggat aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa
    53161 agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca
    53221 tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg
    53281 aagataccaa atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg
    53341 ccaagcaaat tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt
    53401 gtcagcaaaa cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca
    53461 taccccgcaa gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact
    53521 gtatttttga tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg
    53581 gtatagctat ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa
    53641 gaagaaataa tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta
    53701 atcgttcgaa tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc
    53761 gagctttctg gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat
    53821 gtacacatta gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc
    53881 tgatgcatag cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc
    53941 tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg
    54001 catcgaacca atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag
    54061 cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa
    54121 agagggagag attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt
    54181 ccaaacagtc atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg
    54241 gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa
    54301 catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga
    54361 acgacaaaaa cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac
    54421 gataatttgt gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac
    54481 tgctcgttca tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt
    54541 aaagaaaata ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt
    54601 aaataataat aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg
    54661 attccaatat ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata
    54721 tacaatcttg gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca
    54781 ataacaccta tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc
    54841 tatcctgtat aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc
    54901 gttcatgagt gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat
    54961 atcgttattg atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat
    55021 ttgtttcgat aattcagata caataggatc attttttact tcttcatctt ttgctactga
    55081 atccaagtag gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt
    55141 ccaatatttc aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat
    55201 ggcatatcct acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa
    55261 ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt
    55321 gatttcaatg gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga
    55381 ttctggacca gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt
    55441 ctcaggagac gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc
    55501 taacatccag ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt
    55561 tttactacta tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg
    55621 ttgttcagta tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc
    55681 ttgtacctgc atggggacaa aatactgttt accttctact tcacctaatt caatagccgt
    55741 ttctttaaaa tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac
    55801 cgctccgttt tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc
    55861 ttttttagaa gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc
    55921 catttcaatt ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa
    55981 tatttcacca tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga
    56041 atcagattct gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa
    56101 caaagagcca attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac
    56161 gacatctgac cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag
    56221 cttttacatt tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt
    56281 ttgtatcaaa tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca
    56341 agtgaacagt tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt
    56401 tgtcatttat tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat
    56461 agtttgctga acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca
    56521 taaatggata atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg
    56581 caataacatt taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata
    56641 aagtgattaa tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta
    56701 aaggaggagc aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa
    56761 tattccattc atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg
    56821 tatcgcacat cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat
    56881 aatgagaaag atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt
    56941 gatcgtacgc ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat
    57001 tcgaacttcc gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg
    57061 tttcctctgc taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt
    57121 ccatttcatt ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac
    57181 gccttaaatc actccaataa gttcctttag gaaaattaca atattcgact aaattacgag
    57241 ctgcatctac tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct
    57301 gacaccagtt ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta
    57361 aatccaacct tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg
    57421 acacaaaatt tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa
    57481 tgacgtcttg gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt
    57541 catatactgc tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc
    57601 ctacacgctt gacactacta atcaacattg tggcttccat aactgacctc ctttaattta
    57661 tacatgaata atacacctcg ttgaaagcgt tgtcaataat ataaatgtta aacttactta
    57721 ttttttatcg attttaatta accaaaatat taataatgat taaaacaaat aatttaatat
    57781 aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg
    57841 caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg
    57901 atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa
    57961 cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc
    58021 atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat
    58081 atcagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt
    58141 ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt
    58201 atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt
    58261 ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat
    58321 acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac
    58381 aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc
    58441 cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt
    58501 aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga
    58561 gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg
    58621 cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga
    58681 ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt
    58741 ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg
    58801 ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt
    58861 ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc
    58921 atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc
    58981 ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag
    59041 caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc
    59101 accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc
    59161 tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt
    59221 tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc
    59281 aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc
    59341 ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata
    59401 gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc
    59461 taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt
    59521 tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt
    59581 tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat
    59641 gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg
    59701 ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac
    59761 tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt
    59821 aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag
    59881 tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct
    59941 tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt
    60001 cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat
    60061 aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact
    60121 gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca
    60181 attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca
    60241 tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata
    60301 taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct
    60361 ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag
    60421 aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa
    60481 gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca
    60541 cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc ccagactgct
    60601 gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca
    60661 gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt
    60721 gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggattaa tggttcattt
    60781 gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt
    60841 aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa
    60901 tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg
    60961 tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct
    61021 cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc
    61081 aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt
    61141 tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa
    61201 agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt
    61261 tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac
    61321 acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta
    61381 caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat
    61441 aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc
    61501 aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag
    61561 atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa
    61621 agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg
    61681 ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat gattattagt
    61741 cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga
    61801 cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca
    61861 tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt
    61921 tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt
    61981 tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat
    62041 tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt
    62101 agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta
    62161 ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta
    62221 ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat tttttcttga
    62281 ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca
    62341 caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg
    62401 agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa
    62461 aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg
    62521 atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc
    62581 agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga
    62641 cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta
    62701 aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg
    62761 gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg
    62821 aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac
    62881 cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg ttctcttaca
    62941 gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg
    63001 aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg
    63061 aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat
    63121 gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg
    63181 ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt
    63241 ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc
    63301 gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt
    63361 atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc
    63421 aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc
    63481 ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg
    63541 aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg
    63601 ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt
    63661 aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca
    63721 aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga
    63781 cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt
    63841 ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc
    63901 aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc
    63961 aatgcttttc gtttttccat aacttaactg tctagcattt gcattaggga tataatcgac
    64021 ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt
    64081 gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt
    64141 tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct
    64201 ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg
    64261 ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata
    64321 ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac
    64381 gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc
    64441 cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt
    64501 tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaattgaa
    64561 tacattataa tgatactgta aatatatctt tattattagt gaat
//
LOCUS       DACDOA010000003        62847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDOA010000003 DACDOA010000000
VERSION     DACDOA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.003"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..484
                     /locus_tag="IWI39_000126"
     CDS             <1..484
                     /locus_tag="IWI39_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9207264.1"
                     /translation="EYILGIILYRDSIVLDGITFPDKNLFQHLRQQFPPVHSNLDTWL
                     DRYSKLEPEMQEFIANKTCNKVKNEKISLFNILKIEELFINAIVEEFDNNILFLTSSI
                     EELLERMVDEWLPIQLKDMSYPEEAVREITDNINKLEQTISSIGKKYTKKKMKGGDTI
                     "
     gene            523..1665
                     /locus_tag="IWI39_000127"
     CDS             523..1665
                     /locus_tag="IWI39_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9207265.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(1760..3151)
                     /gene="sufB"
                     /locus_tag="IWI39_000128"
     CDS             complement(1760..3151)
                     /gene="sufB"
                     /locus_tag="IWI39_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9207266.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3156..3626)
                     /locus_tag="IWI39_000129"
     CDS             complement(3156..3626)
                     /locus_tag="IWI39_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9207267.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(3613..4848)
                     /locus_tag="IWI39_000130"
     CDS             complement(3613..4848)
                     /locus_tag="IWI39_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9207268.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(4845..6131)
                     /gene="sufD"
                     /locus_tag="IWI39_000131"
     CDS             complement(4845..6131)
                     /gene="sufD"
                     /locus_tag="IWI39_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9207269.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6144..6917)
                     /gene="sufC"
                     /locus_tag="IWI39_000132"
     CDS             complement(6144..6917)
                     /gene="sufC"
                     /locus_tag="IWI39_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9207270.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(7114..7962)
                     /locus_tag="IWI39_000133"
     CDS             complement(7114..7962)
                     /locus_tag="IWI39_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207271.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(7986..8672)
                     /locus_tag="IWI39_000134"
     CDS             complement(7986..8672)
                     /locus_tag="IWI39_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207272.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(8665..9699)
                     /locus_tag="IWI39_000135"
     CDS             complement(8665..9699)
                     /locus_tag="IWI39_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9207273.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(10094..10432)
                     /locus_tag="IWI39_000136"
     CDS             complement(10094..10432)
                     /locus_tag="IWI39_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9207274.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(10434..10790)
                     /locus_tag="IWI39_000137"
     CDS             complement(10434..10790)
                     /locus_tag="IWI39_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9207275.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(10796..11983)
                     /locus_tag="IWI39_000138"
     CDS             complement(10796..11983)
                     /locus_tag="IWI39_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9207276.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(12174..13019)
                     /locus_tag="IWI39_000139"
     CDS             complement(12174..13019)
                     /locus_tag="IWI39_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9207277.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            13253..14218
                     /locus_tag="IWI39_000140"
     CDS             13253..14218
                     /locus_tag="IWI39_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9207278.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            14595..15638
                     /locus_tag="IWI39_000141"
     CDS             14595..15638
                     /locus_tag="IWI39_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9207279.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(15700..16164)
                     /locus_tag="IWI39_000142"
     CDS             complement(15700..16164)
                     /locus_tag="IWI39_000142"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9207280.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            16410..17357
                     /locus_tag="IWI39_000143"
     CDS             16410..17357
                     /locus_tag="IWI39_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9207281.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(17568..19640)
                     /locus_tag="IWI39_000144"
     CDS             complement(17568..19640)
                     /locus_tag="IWI39_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9207282.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(19573..19950)
                     /locus_tag="IWI39_000145"
     CDS             complement(19573..19950)
                     /locus_tag="IWI39_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9207283.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(20079..20639)
                     /locus_tag="IWI39_000146"
     CDS             complement(20079..20639)
                     /locus_tag="IWI39_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9207284.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            20789..22081
                     /locus_tag="IWI39_000147"
     CDS             20789..22081
                     /locus_tag="IWI39_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9207285.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(22141..23259)
                     /locus_tag="IWI39_000148"
     CDS             complement(22141..23259)
                     /locus_tag="IWI39_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9207286.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(23273..24694)
                     /gene="arcD"
                     /locus_tag="IWI39_000149"
     CDS             complement(23273..24694)
                     /gene="arcD"
                     /locus_tag="IWI39_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9207287.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(24940..26061)
                     /gene="mnmA"
                     /locus_tag="IWI39_000150"
     CDS             complement(24940..26061)
                     /gene="mnmA"
                     /locus_tag="IWI39_000150"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9207288.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            26465..27064
                     /locus_tag="IWI39_000151"
     CDS             26465..27064
                     /locus_tag="IWI39_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9207289.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(27202..28488)
                     /gene="efmA"
                     /locus_tag="IWI39_000152"
     CDS             complement(27202..28488)
                     /gene="efmA"
                     /locus_tag="IWI39_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9207290.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(28759..29103)
                     /locus_tag="IWI39_000153"
     CDS             complement(28759..29103)
                     /locus_tag="IWI39_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9207291.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(29240..30376)
                     /locus_tag="IWI39_000154"
     CDS             complement(29240..30376)
                     /locus_tag="IWI39_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9207292.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(30565..31539)
                     /locus_tag="IWI39_000155"
     CDS             complement(30565..31539)
                     /locus_tag="IWI39_000155"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9207293.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(31619..32494)
                     /locus_tag="IWI39_000156"
     CDS             complement(31619..32494)
                     /locus_tag="IWI39_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9207294.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            32624..34531
                     /locus_tag="IWI39_000157"
     CDS             32624..34531
                     /locus_tag="IWI39_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9207295.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(35072..38350)
                     /locus_tag="IWI39_000158"
     CDS             complement(35072..38350)
                     /locus_tag="IWI39_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9207296.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(38621..39166)
                     /locus_tag="IWI39_000159"
     CDS             complement(38621..39166)
                     /locus_tag="IWI39_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9207297.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(39178..39870)
                     /locus_tag="IWI39_000160"
     CDS             complement(39178..39870)
                     /locus_tag="IWI39_000160"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9207298.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(39889..40173)
                     /locus_tag="IWI39_000161"
     CDS             complement(39889..40173)
                     /locus_tag="IWI39_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207299.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(40186..40749)
                     /locus_tag="IWI39_000162"
     CDS             complement(40186..40749)
                     /locus_tag="IWI39_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9207300.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            40977..41639
                     /locus_tag="IWI39_000163"
     CDS             40977..41639
                     /locus_tag="IWI39_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9207301.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            41763..42959
                     /locus_tag="IWI39_000164"
     CDS             41763..42959
                     /locus_tag="IWI39_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9207302.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(43060..44436)
                     /locus_tag="IWI39_000165"
     CDS             complement(43060..44436)
                     /locus_tag="IWI39_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207303.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(44473..45339)
                     /locus_tag="IWI39_000166"
     CDS             complement(44473..45339)
                     /locus_tag="IWI39_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9207304.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(45428..46453)
                     /locus_tag="IWI39_000167"
     CDS             complement(45428..46453)
                     /locus_tag="IWI39_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9207305.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(46471..47145)
                     /locus_tag="IWI39_000168"
     CDS             complement(46471..47145)
                     /locus_tag="IWI39_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9207306.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(47329..48222)
                     /locus_tag="IWI39_000169"
     CDS             complement(47329..48222)
                     /locus_tag="IWI39_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9207307.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(48296..49993)
                     /locus_tag="IWI39_000170"
     CDS             complement(48296..49993)
                     /locus_tag="IWI39_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9207308.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(49986..51299)
                     /locus_tag="IWI39_000171"
     CDS             complement(49986..51299)
                     /locus_tag="IWI39_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9207309.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(51299..55336)
                     /locus_tag="IWI39_000172"
     CDS             complement(51299..55336)
                     /locus_tag="IWI39_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9207310.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(55349..55807)
                     /locus_tag="IWI39_000173"
     CDS             complement(55349..55807)
                     /locus_tag="IWI39_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9207311.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(56105..56623)
                     /locus_tag="IWI39_000174"
     CDS             complement(56105..56623)
                     /locus_tag="IWI39_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9207312.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(56625..57242)
                     /locus_tag="IWI39_000175"
     CDS             complement(56625..57242)
                     /locus_tag="IWI39_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9207313.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(57239..58093)
                     /locus_tag="IWI39_000176"
     CDS             complement(57239..58093)
                     /locus_tag="IWI39_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207314.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(58090..59001)
                     /locus_tag="IWI39_000177"
     CDS             complement(58090..59001)
                     /locus_tag="IWI39_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207315.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(59042..60376)
                     /locus_tag="IWI39_000178"
     CDS             complement(59042..60376)
                     /locus_tag="IWI39_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207316.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            60584..61414
                     /locus_tag="IWI39_000179"
     CDS             60584..61414
                     /locus_tag="IWI39_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9207317.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(61658..61907)
                     /locus_tag="IWI39_000180"
                     /pseudo
     CDS             complement(61658..61907)
                     /locus_tag="IWI39_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(62098..62292)
                     /locus_tag="IWI39_000181"
     CDS             complement(62098..62292)
                     /locus_tag="IWI39_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207318.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17946 a        13429 c        11299 g        20173 t
ORIGIN      
        1 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
       61 tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt
      121 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
      181 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
      241 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
      301 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
      361 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
      421 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
      481 ctagtggtaa gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag
      541 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
      601 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
      661 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
      721 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
      781 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
      841 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
      901 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
      961 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
     1021 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
     1081 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
     1141 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
     1201 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
     1261 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
     1321 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
     1381 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
     1441 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
     1501 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
     1561 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
     1621 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
     1681 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
     1741 tctagctcct ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat
     1801 tgagttcgac tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga
     1861 cgatcatttc agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt
     1921 cttctgagat ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct
     1981 cattgaatgg gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga
     2041 tatgagagat cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt
     2101 ctccgccgtc ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt
     2161 ggatcatttt tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca
     2221 tggtgcctcg tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg
     2281 cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg
     2341 ttactaagtt gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat
     2401 cttttcttgt aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg
     2461 ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt
     2521 cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt
     2581 tgacgccttt tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg
     2641 ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt
     2701 attctttcaa agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt
     2761 tcatattatg gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc
     2821 gttcagcttc tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt
     2881 cccagtcacg agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga
     2941 tgtctgaaag gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt
     3001 tcagacggaa ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca
     3061 cttcttctgt caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga
     3121 atttatattc ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga
     3181 tggaaagctg cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc
     3241 gcacatttga tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat
     3301 tggtcgggat ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt
     3361 tctgcttctt ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta
     3421 ctgatggagc agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg
     3481 acttgcaatt cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag
     3541 tcagtcaagc ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat
     3601 aaattgtcta atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc
     3661 aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc
     3721 tgatacttca aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac
     3781 cccttccatg tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc
     3841 aacgactcct gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc
     3901 ttgtaatttt ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat
     3961 accgagtttt tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg
     4021 tgtccctgct tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa
     4081 atcgatcatt tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc
     4141 atataaaaca ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc
     4201 acagtctagt gcttggacat caatggccat gtgcggggca gattgagcac catctactac
     4261 gatgaccccg ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc
     4321 taagacattg gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc
     4381 tgcatcctcc atatccagga agccttcttc tgttagttcg atatatttca agacagcacc
     4441 tgttcgtttt gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag
     4501 cactatctcg tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa
     4561 gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc
     4621 ccgtactttt tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac
     4681 accgcgatga acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg
     4741 ttttggcttt tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc
     4801 ttgaaataag ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc
     4861 ccttcgatta cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca
     4921 gaaccgagga atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc
     4981 atcagatagt acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt
     5041 acttcgtttt catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc
     5101 atcaagacac gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg
     5161 ataccattaa aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg
     5221 atcgtatgag gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag
     5281 ctgattgcca cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag
     5341 tcagcgatca catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt
     5401 aaaatgtgcc cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct
     5461 gaatatttga tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat
     5521 tttgctactt gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca
     5581 tctgccacga tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat
     5641 aaggactcga gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg
     5701 ttcataaaag ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc
     5761 atataatatt cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa
     5821 atgactcctt tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt
     5881 tggacaagca caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg
     5941 atggtttgtg tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata
     6001 tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa
     6061 tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt
     6121 ttttctttca ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct
     6181 ttactgatac cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc
     6241 atgactactc ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat
     6301 cgttgatagt gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct
     6361 ttagcaacga ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca
     6421 aatgtaggtt ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa
     6481 aaaccttcgt tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt
     6541 tcatctaact ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca
     6601 ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc
     6661 atagctaaaa ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc
     6721 ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca
     6781 gataaggtag attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc
     6841 attgttaggt ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt
     6901 atttctaaga cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga
     6961 ctaattgaga atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta
     7021 ttaaacaatt ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta
     7081 cagacttatt tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt
     7141 aacagagccg ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc
     7201 aatcagtttt ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac
     7261 gtaaggagag ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc
     7321 gtaagcgaag tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc
     7381 taaaagtgat gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc
     7441 atcgaatgtt gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg
     7501 tagaattgtg atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc
     7561 atcttttaat tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg
     7621 gacagcaccg gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg
     7681 aatatgctgg aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac
     7741 atagtcatcg aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac
     7801 atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc
     7861 tgctttattt gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt
     7921 cagcaaaagc gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata
     7981 tcctattatc ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat
     8041 acgattagta aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag
     8101 ccttcctgcc aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta
     8161 aatccgatca tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca
     8221 ggtatcaaga ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc
     8281 agtacaccag ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct
     8341 gcggataaaa caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct
     8401 ttagtaaatg gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta
     8461 ctgatgatcg atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata
     8521 gagtaaagga tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat
     8581 gtcatatata gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa
     8641 tctagatatg tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg
     8701 ttctttgatg aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt
     8761 caatgaacca aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc
     8821 gatgttatag cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa
     8881 ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt
     8941 cgaatcagct tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg
     9001 ttgtctgaag acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc
     9061 gactttgttg cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa
     9121 attcaatttc ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc
     9181 caatgcgcta gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc
     9241 gataccgacg cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc
     9301 ttctaacccg actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac
     9361 gccagctaat tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt
     9421 gaaatgctgg aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa
     9481 ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt
     9541 caggagacgg actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc
     9601 ccctttttca acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt
     9661 gaatgtcttt ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta
     9721 taatttttca aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga
     9781 acttccgtgt taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt
     9841 ggacttagct tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc
     9901 ttacgctcgc ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca
     9961 gcaaacaggg ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt
    10021 ttcatatagg attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag
    10081 tgattttctg attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga
    10141 tccacgcatt catttcatct gaatcattca agatattgat atcttcaccg attttatcat
    10201 taacagaaga aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt
    10261 caacttcgat cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa
    10321 acgtgatgtc tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc
    10381 cattaaataa gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc
    10441 agttctcctt cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag
    10501 atcagtggtc gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc
    10561 gtaaactcgc ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg
    10621 aacaggcgac ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc
    10681 ggtacttctt tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct
    10741 ttggcttttt tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc
    10801 tccttgaaga tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac
    10861 cgatcatgtt ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg
    10921 ttaaaggaag taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca
    10981 tgatcaaacc gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa
    11041 tcatacgata gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga
    11101 agttttcacc gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc
    11161 ttacgttgaa tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg
    11221 gctgataact cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa
    11281 actgataaga tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca
    11341 agaagatcgt tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc
    11401 aagagattcc agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc
    11461 caaagtcatc ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc
    11521 caatcagcca aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta
    11581 cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa
    11641 acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga
    11701 atttgagcaa gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc
    11761 agatccattt ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc
    11821 agacaacttg catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg
    11881 ctacatataa tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat
    11941 aatcaatacg attatcgttc gaagctttta attttttttc catgtttcat cccttttctt
    12001 aaaaaccata ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag
    12061 acatttggga aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag
    12121 cctactgctt atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag
    12181 ctgagattct cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg
    12241 gcaatctcta gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga
    12301 tggaaattca acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag
    12361 cgaccgattc cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc
    12421 ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt
    12481 gcttctctta caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc
    12541 atcaaagagt atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg
    12601 gattcatgtt tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa
    12661 tgcactttag gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca
    12721 aaggtgtggg aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt
    12781 tgctgagttt cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct
    12841 tgcttagttg gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc
    12901 cgctccaact gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct
    12961 gctctggaaa aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg
    13021 gcaccacttc cataactttt tatgataatt actataaaaa ctatttgatt taaatataca
    13081 aaaaaccatc tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag
    13141 atgaagacaa tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt
    13201 attcgttcat cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa
    13261 ctttttgaaa attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg
    13321 aaatctgttt aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat
    13381 gattcttatg atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac
    13441 gttgcatgat ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat
    13501 ttgtacttat tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg
    13561 cgctgttatc ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc
    13621 tgatctttca attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt
    13681 catcactgta actgccggga acttcttccc acttattgga aaaattttct tttggatttc
    13741 attgatctgc tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa
    13801 tatggctcat ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct
    13861 aacaggttat cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc
    13921 tttatctcaa gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg
    13981 tttttatcca agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt
    14041 tactactgtt cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa
    14101 agttgcggaa tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg
    14161 tttcttaatt aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata
    14221 atcgattcaa taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa
    14281 aaacagctgt tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa
    14341 caccgtttac caagaggtcg taaaagaagt ggtcagcttc aagaaataag aaggaattta
    14401 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg
    14461 gaacaccgtt tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt
    14521 ttgtcaaaat actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga
    14581 aaggaaccga tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg
    14641 tccgcgaaat cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc
    14701 ttgctattta tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat
    14761 taggaaatac tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca
    14821 aactattaga attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc
    14881 aaattggtgt aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg
    14941 cttacttgat tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag
    15001 gaaacgctgt ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag
    15061 aggaagaaaa aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga
    15121 ctctcccttt tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt
    15181 tgggtggtac actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt
    15241 ctgttgtcga attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg
    15301 tctatctgtt tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg
    15361 atacgcagaa aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt
    15421 gtttggtcaa cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca
    15481 gcagctggtt tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat
    15541 tcatgaaaga aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag
    15601 tgatggcttt actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg
    15661 tgattctcgc atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc
    15721 taacaacaac aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt
    15781 cagttttcca ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt
    15841 tcgatttgcc tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc
    15901 tttatacgtt cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg
    15961 aaaaggcgta cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa
    16021 atattcttca aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc
    16081 tgaaattggc tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa
    16141 gccgataggt gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc
    16201 ctttttctct atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa
    16261 accgaggaaa catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac
    16321 gtaaaatcaa attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat
    16381 ataaacatac gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta
    16441 atgaagaaaa aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg
    16501 agttcgaata tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga
    16561 agctagcctc agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag
    16621 aagcggctct gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg
    16681 ttgatctgca agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg
    16741 atatcgattg tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt
    16801 tctttgcgaa aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg
    16861 gcgctcgtga tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag
    16921 aatacaaccg attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt
    16981 cttatgaaaa ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca
    17041 actactcgat tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta
    17101 tcggcgcttt gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat
    17161 tgttgtttgg tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg
    17221 gtggaatcca attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag
    17281 aaacgaaaca gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat
    17341 tgaagcctca ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac
    17401 tgtgacaaaa gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt
    17461 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    17521 tatttcttga agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt
    17581 gctgctttta gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt
    17641 ttcagcaagc gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc
    17701 aatttttctg tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg
    17761 atttccattt tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt
    17821 ctgcggtagt tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc
    17881 tcgcgcagat tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat
    17941 tcttgtttgc ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc
    18001 aagaaggttc ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt
    18061 tctgtcaaat ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta
    18121 ggagatactt gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg
    18181 ataatctctg cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag
    18241 cttgcttctg acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga
    18301 ttgattctca ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc
    18361 catcctgctt ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc
    18421 gtaacttttg tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca
    18481 atcatttgat agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca
    18541 gttggcaaaa gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg
    18601 acgatggctc cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc
    18661 aatcgttctt tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga
    18721 taagtaacga tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca
    18781 gaatagccat agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc
    18841 gcagattccg tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc
    18901 aattgaacaa gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc
    18961 attttggctt gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac
    19021 tgctcgattt tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct
    19081 gctgataaat tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc
    19141 cagtccgctt ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt
    19201 cttagttgct tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc
    19261 ttcactggtt tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct
    19321 cttccggcat ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg
    19381 attgctttta attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc
    19441 attggcaacg tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc
    19501 aatcctaata aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt
    19561 ttgtgctttt gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc
    19621 gctagaatta gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt
    19681 tttcaacaac gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg
    19741 ctcatctccg tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata
    19801 attttgatgg atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc
    19861 gaatgtttct tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt
    19921 gtccccgaac atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga
    19981 ctcatcttag tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta
    20041 atgaacatct taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag
    20101 ttattgatca tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat
    20161 cctaaaaaac gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga
    20221 actttttctt ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt
    20281 tcatggattc catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca
    20341 tacactgctt gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct
    20401 tcctgaccac tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt
    20461 ttcgctgtta gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga
    20521 gccttgatat ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct
    20581 tctgctagtt ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt
    20641 tccatgtttc ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag
    20701 agaaaaaaga gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga
    20761 tgaaaatttt cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag
    20821 gaaaagagaa acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg
    20881 tatcggattt agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta
    20941 tagtacgtca caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac
    21001 tacgcttatc tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct
    21061 gttgcgtgct tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt
    21121 gtatcaatta gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc
    21181 cactgccctt gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt
    21241 agcaactggt tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc
    21301 agcgtttggc tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt
    21361 gttgagtctg tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc
    21421 tgccaaacag attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac
    21481 aacgatgatc atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg
    21541 ccaattgctg catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc
    21601 tgggattgcc actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag
    21661 tgaaagaata cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt
    21721 cgtcacgaat gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg
    21781 tctgctccct gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg
    21841 gatcttcagt tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg
    21901 ctccagtgtt tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt
    21961 tttggtcaac ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc
    22021 agaagaaaaa gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata
    22081 agcgtaaatg aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt
    22141 ttaggcctgt tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt
    22201 tttacaacag taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa
    22261 tagtactcct gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc
    22321 taatttaatg aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt
    22381 ggctacccat tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa
    22441 tattttttct ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca
    22501 aatcatcgtg tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt
    22561 ccagccaata cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc
    22621 tttttcatag acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac
    22681 ttcatctatc aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt
    22741 tttccgatct agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt
    22801 ttcaggtttg actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt
    22861 caaatgaaca aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata
    22921 agtaggaagc atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag
    22981 attagcacca gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag
    23041 agaagcgact tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc
    23101 gtttttgcgt ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc
    23161 taatgtcaat gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt
    23221 atttagccat tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct
    23281 tgatccagcc tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa
    23341 tcattacttt ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga
    23401 agcaaaaacc aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa
    23461 tgatgcccat caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc
    23521 cttcttttaa agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg
    23581 ccgctgtaca taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata
    23641 agaatagatt agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat
    23701 tcgtttttcc ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg
    23761 ctgggagcat cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga
    23821 tcagcgctgc tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg
    23881 aaatagttgc taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat
    23941 agataaacaa taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct
    24001 tcgcgcggtt ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca
    24061 tcaaacaacc tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat
    24121 tatcagatac gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata
    24181 caaatattaa aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga
    24241 cgctcgctcc ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg
    24301 caaataaaat agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg
    24361 cgctcatcag catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc
    24421 aaccactgat gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac
    24481 catctagatc tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac
    24541 caatcccaac gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat
    24601 cacttgtgat cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc
    24661 cccatttgct aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg
    24721 aacaaatcac ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc
    24781 aactttattc atattattgg atattatgaa ctttttacac aagtatttca agtataaaat
    24841 gaataaaaat taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc
    24901 ttttgttagc catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt
    24961 tgtttcttga tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa
    25021 tacaactgct tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg
    25081 attatcatcc aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc
    25141 tgtacacttg aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc
    25201 caagcttgtg gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt
    25261 cgctagatct tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc
    25321 tagtccttga cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc
    25381 accatcaaga gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt
    25441 gaagttcttt tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag
    25501 gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat
    25561 cgtttttgaa agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt
    25621 gtctactcca cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg
    25681 tccagtagct acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt
    25741 gatttctttg ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag
    25801 gaaatattca aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat
    25861 cccgatttga gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc
    25921 gttttcgtcc gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc
    25981 ctttaacaaa agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg
    26041 tgttttgctg ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac
    26101 gagttgaagg tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca
    26161 tcccttaatt atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat
    26221 gatagtcaac caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc
    26281 ataaaaaggg ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt
    26341 ttcaaaattg cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag
    26401 atgttatgag ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag
    26461 atacatgaat tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa
    26521 cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag
    26581 ctggaatgca gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca
    26641 tccgcaatat gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac
    26701 aatccacttg atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa
    26761 tgatcctagg cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt
    26821 taaattcgac catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga
    26881 cactatccgc aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt
    26941 caatgtaggc gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac
    27001 tccgttatct gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc
    27061 gtgaaaaaac tgccctatat caagtattaa acaaataaag ataattactc atatttttca
    27121 tttgagtagg cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt
    27181 ctgtcacaac tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt
    27241 tttctgtaat tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca
    27301 taataaaatc ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa
    27361 tggtcctgca aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc
    27421 taagacacga cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt
    27481 attgaaatat ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc
    27541 ttgactatcg tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc
    27601 tataataata ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc
    27661 taagagcatt ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc
    27721 aaagtaatct agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac
    27781 aaatgcacca ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc
    27841 aaattttgca tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg
    27901 gatcttcaca aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat
    27961 aaggtaattg acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa
    28021 attcgctgat tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac
    28081 gattgtcggc ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat
    28141 aaacaaagac acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc
    28201 taaaataagc gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc
    28261 ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag
    28321 taaagtagca aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat
    28381 cgcatattgg acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa
    28441 ataatttctc ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct
    28501 tttctttttc attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa
    28561 aaaatacctt tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa
    28621 taaatcagca aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac
    28681 taatttgcgt cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg
    28741 tctttttata tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc
    28801 catcagaaaa taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac
    28861 tgctttcaat ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac
    28921 agtgattttt aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa
    28981 ttgataattg cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc
    29041 atcaggatgc aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc
    29101 catgattttc acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt
    29161 actaggtttt tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat
    29221 ataagaatga cttcactagt tattttttga cagcacgaac taattgatca aggaaatact
    29281 ggatgtcgtc ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg
    29341 gtgtatcttc tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac
    29401 aagctgaacc agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat
    29461 ttgcttcgcc aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat
    29521 ttacttgata agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt
    29581 tatatttgtt gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca
    29641 taccccagat ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc
    29701 ctaaaagcaa tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg
    29761 gcccattgat tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga
    29821 tcgtttggtt tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta
    29881 aaagttcacc gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca
    29941 cagagaccaa gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc
    30001 ctttttcatc tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg
    30061 tattaagaac agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac
    30121 gcgagaacgc tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga
    30181 tgatctcgtg aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt
    30241 tttcatgcgc gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca
    30301 tgattgccgt catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca
    30361 gataaatagt tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata
    30421 aaatagcgaa caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag
    30481 aaaaaaggag ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac
    30541 aacttctttc cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg
    30601 actcatcggt gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt
    30661 aacgattgtt aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca
    30721 aatatctttg attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg
    30781 tgacgcacag gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc
    30841 aagcgttgct cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc
    30901 accgatcaca taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt
    30961 gctgttcaaa tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac
    31021 aactacgatg tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat
    31081 cgtaaataaa ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc
    31141 taaagtcagt actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt
    31201 gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac
    31261 gacattgatc gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat
    31321 atagtaatca ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat
    31381 actttcttcg atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc
    31441 taattctgtt ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact
    31501 aaaaatcttc aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct
    31561 ctattcttcg atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt
    31621 aaggaagtga aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc
    31681 agttgtcgtg tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg
    31741 tcacccaatg attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt
    31801 tgccttcacg tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac
    31861 gctcatgatt gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa
    31921 tcgtaggaag tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat
    31981 cagccgtatt tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat
    32041 aataggcaag gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc
    32101 atttcttata cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct
    32161 ttcctgacct tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag
    32221 ataactcgat tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat
    32281 aagtatattt tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac
    32341 tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct
    32401 gagtgctggc taatttttta atttttttat ctactttgaa atataaaggt ttatccacta
    32461 cttgattcag ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa
    32521 atcagtttga ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt
    32581 gtaagataat gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt
    32641 gaaacaagat ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc
    32701 agataaacca gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat
    32761 gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca
    32821 tttccttgca tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca
    32881 accgcttcaa cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa
    32941 gcaatttcca gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc
    33001 agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc
    33061 agataagacg tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt
    33121 tgatatggct gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat
    33181 cacggaacaa gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga
    33241 aaacgcagat tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac
    33301 agataaactg gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt
    33361 gatcgtaact gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt
    33421 tccgttattc aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc
    33481 agaagaattc cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc
    33541 aatgcctcag aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg
    33601 cgattactac ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt
    33661 gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc
    33721 aacaaaagaa aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga
    33781 taagatccca gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt
    33841 gctttcaaat actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa
    33901 caaaccagtg gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca
    33961 tccatttcgg aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct
    34021 agaacaatca agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc
    34081 ccatgcattt gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg
    34141 gacagaagaa gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga
    34201 tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc
    34261 tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat
    34321 cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca
    34381 acaactttta ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc
    34441 aaaaaacctt gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct
    34501 agcgccagaa aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa
    34561 gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt
    34621 ttcgaagaag ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt
    34681 caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta
    34741 ctaccgagaa actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga
    34801 cctgagtatt agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct
    34861 aataccgagg gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct
    34921 gcatcaagta ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga
    34981 tagaaggaag tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa
    35041 ttgttttaca tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa
    35101 caactacacc gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc
    35161 ccgtttttgg gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat
    35221 gagaaggttt tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg
    35281 gtttgtcatt tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg
    35341 taacagattc tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga
    35401 cttctagcgt atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt
    35461 gagcacttga ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa
    35521 agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt
    35581 caatgatatt tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat
    35641 tattttgttc atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt
    35701 ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca
    35761 ctctttcctt ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga
    35821 tacttccatt ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag
    35881 actcgatcca atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt
    35941 cattccaata gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga
    36001 tctgctctct catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt
    36061 ctgtgtttcc tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg
    36121 tactgctatt gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt
    36181 cgaaagacaa actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga
    36241 atccattacc gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg
    36301 agtttttgat gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac
    36361 cgtcatctgc attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca
    36421 gtttggccgc aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac
    36481 agttcttgac taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga
    36541 tttggattcc tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc
    36601 cagaaatctg taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac
    36661 cccagtagtg actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac
    36721 gcttgttttc tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa
    36781 cgccttttgg aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg
    36841 tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc
    36901 cgtttcctgc atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt
    36961 agtccactgt atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg
    37021 gcggatagtt tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt
    37081 ttgtctgata atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca
    37141 cctctcctgt tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca
    37201 tagtatgatc ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca
    37261 agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga
    37321 ttccttctac cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt
    37381 catctgaagc gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg
    37441 tttcaggatc gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca
    37501 gtttagtaat cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat
    37561 aagaacctgt ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg
    37621 agacactccc aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc
    37681 gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc
    37741 tatccgagtc ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa
    37801 atgcctgccc aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt
    37861 cttgggcatt cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga
    37921 tgtctttggg gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt
    37981 ttccgccgtt attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc
    38041 tactagcact tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag
    38101 attgcttttt tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg
    38161 tatccatatc ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca
    38221 cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa
    38281 ttaaaaccgg ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt
    38341 tttttaacaa gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct
    38401 aatttatttt tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa
    38461 aaatataact taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt
    38521 ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta
    38581 ttattttgtg ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta
    38641 gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg
    38701 aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca
    38761 cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac
    38821 ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat
    38881 tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac
    38941 ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg
    39001 gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat
    39061 gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct
    39121 ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta
    39181 tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat
    39241 aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat
    39301 caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca
    39361 agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa
    39421 tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt
    39481 aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg
    39541 tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat
    39601 aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac
    39661 catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc
    39721 tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc
    39781 gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc
    39841 ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc
    39901 gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa
    39961 agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt
    40021 ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt
    40081 ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag
    40141 ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt
    40201 caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt
    40261 catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc
    40321 ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc
    40381 atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata
    40441 gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg
    40501 atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt
    40561 gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa
    40621 taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc
    40681 aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt
    40741 gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa
    40801 tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat
    40861 agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt
    40921 aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga
    40981 taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca
    41041 atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac
    41101 tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac
    41161 ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa
    41221 caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca
    41281 aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag
    41341 aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc
    41401 cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca
    41461 aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa
    41521 tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat
    41581 ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac
    41641 tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact
    41701 ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt
    41761 ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag
    41821 atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg
    41881 agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg
    41941 aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg
    42001 tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga
    42061 atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc
    42121 gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg
    42181 tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg
    42241 cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata
    42301 atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt
    42361 atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga
    42421 tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc
    42481 aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga
    42541 tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga
    42601 tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga
    42661 ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg
    42721 acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa
    42781 atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa
    42841 caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag
    42901 tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta acccaataga
    42961 tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg
    43021 gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct
    43081 gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga taccgaacac
    43141 gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat
    43201 tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt
    43261 tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt
    43321 tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc
    43381 ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg
    43441 aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat
    43501 cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg
    43561 tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac
    43621 gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc
    43681 aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac
    43741 ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa
    43801 accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat
    43861 aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt
    43921 cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc
    43981 aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc
    44041 taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc
    44101 tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac
    44161 aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa
    44221 acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg
    44281 accagcattt tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc
    44341 accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg
    44401 attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt
    44461 ggaaaacagc gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa
    44521 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
    44581 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
    44641 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
    44701 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
    44761 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
    44821 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
    44881 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
    44941 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
    45001 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
    45061 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
    45121 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
    45181 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
    45241 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
    45301 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
    45361 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
    45421 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
    45481 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
    45541 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
    45601 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
    45661 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
    45721 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
    45781 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
    45841 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
    45901 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
    45961 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
    46021 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
    46081 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
    46141 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
    46201 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
    46261 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
    46321 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
    46381 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
    46441 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
    46501 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
    46561 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
    46621 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
    46681 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
    46741 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
    46801 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
    46861 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
    46921 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
    46981 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
    47041 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
    47101 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
    47161 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
    47221 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
    47281 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
    47341 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
    47401 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
    47461 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
    47521 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
    47581 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
    47641 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
    47701 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
    47761 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
    47821 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
    47881 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
    47941 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
    48001 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
    48061 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
    48121 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
    48181 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
    48241 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
    48301 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
    48361 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
    48421 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
    48481 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
    48541 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    48601 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    48661 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    48721 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    48781 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    48841 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    48901 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    48961 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    49021 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    49081 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    49141 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    49201 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    49261 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    49321 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    49381 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    49441 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    49501 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    49561 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    49621 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    49681 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    49741 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    49801 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    49861 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    49921 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    49981 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    50041 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    50101 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    50161 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    50221 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    50281 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    50341 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    50401 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    50461 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    50521 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    50581 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    50641 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    50701 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    50761 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    50821 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    50881 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    50941 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    51001 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    51061 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    51121 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    51181 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    51241 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    51301 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    51361 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    51421 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    51481 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    51541 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    51601 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    51661 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    51721 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    51781 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    51841 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    51901 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    51961 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    52021 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    52081 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    52141 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    52201 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    52261 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    52321 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    52381 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    52441 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    52501 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    52561 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    52621 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    52681 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    52741 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    52801 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    52861 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    52921 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    52981 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    53041 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    53101 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    53161 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    53221 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    53281 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    53341 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    53401 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    53461 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    53521 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    53581 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    53641 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    53701 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    53761 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    53821 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    53881 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    53941 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    54001 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    54061 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    54121 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    54181 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    54241 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    54301 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    54361 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    54421 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    54481 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    54541 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    54601 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    54661 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    54721 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    54781 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    54841 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    54901 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    54961 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    55021 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    55081 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    55141 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    55201 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    55261 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    55321 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    55381 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    55441 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    55501 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    55561 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    55621 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    55681 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    55741 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    55801 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    55861 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
    55921 tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata aaattttata
    55981 acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc
    56041 tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat
    56101 taattcagat aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta
    56161 aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa
    56221 ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa
    56281 aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga
    56341 caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca
    56401 aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat
    56461 caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat
    56521 tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact
    56581 tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta
    56641 agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg
    56701 aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac
    56761 gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta
    56821 atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg
    56881 cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa
    56941 tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct
    57001 tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt
    57061 ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct
    57121 aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga
    57181 atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc
    57241 atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga
    57301 acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg
    57361 tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt
    57421 ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa
    57481 tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat
    57541 tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac
    57601 gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag
    57661 tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg
    57721 ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct
    57781 tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag
    57841 tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat
    57901 agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca
    57961 ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta
    58021 ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga
    58081 aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa
    58141 attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata
    58201 acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga
    58261 atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa
    58321 agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt
    58381 aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga
    58441 tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat
    58501 tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg
    58561 gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat
    58621 ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa
    58681 tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt
    58741 cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa
    58801 atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt
    58861 caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac
    58921 caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt
    58981 tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg
    59041 attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt
    59101 ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat
    59161 ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt
    59221 gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg
    59281 tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag
    59341 aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca
    59401 agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca
    59461 tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat
    59521 tttgggcacg catggctact aatccggaaa taaatggatc agatacacca gacccaaatt
    59581 cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact
    59641 ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac
    59701 tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa
    59761 aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta
    59821 attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt
    59881 tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat
    59941 attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt
    60001 tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta
    60061 caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac
    60121 cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa
    60181 tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat
    60241 tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg
    60301 cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa
    60361 acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat
    60421 aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt
    60481 aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt
    60541 agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa
    60601 agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca
    60661 ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg
    60721 ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga
    60781 aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt
    60841 tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat
    60901 gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa
    60961 tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa
    61021 tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga
    61081 tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct
    61141 atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac
    61201 tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg
    61261 agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata
    61321 tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc
    61381 aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt
    61441 tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca
    61501 aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg
    61561 gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag
    61621 gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat
    61681 ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt
    61741 gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt
    61801 ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt
    61861 atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg
    61921 gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc
    61981 tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag
    62041 aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta
    62101 tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata
    62161 aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact
    62221 agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag
    62281 tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt
    62341 actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc
    62401 aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat
    62461 ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata cataaccttg
    62521 atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct
    62581 aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa
    62641 gtatctgaac tattaatttt taaactatca tttttatcct caactacatg cacatctgtt
    62701 gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct
    62761 tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc
    62821 aacagcaaaa aatacaatga aaaacaa
//
LOCUS       DACDOA010000004        56377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDOA010000004 DACDOA010000000
VERSION     DACDOA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.004"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(171..1739)
                     /locus_tag="IWI39_000182"
     CDS             complement(171..1739)
                     /locus_tag="IWI39_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9207319.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1743..2153)
                     /locus_tag="IWI39_000183"
     CDS             complement(1743..2153)
                     /locus_tag="IWI39_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9207320.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2150..3073)
                     /locus_tag="IWI39_000184"
     CDS             complement(2150..3073)
                     /locus_tag="IWI39_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9207321.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(3070..3948)
                     /locus_tag="IWI39_000185"
     CDS             complement(3070..3948)
                     /locus_tag="IWI39_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9207322.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3935..5272)
                     /locus_tag="IWI39_000186"
     CDS             complement(3935..5272)
                     /locus_tag="IWI39_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9207323.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5275..6378)
                     /locus_tag="IWI39_000187"
     CDS             complement(5275..6378)
                     /locus_tag="IWI39_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9207324.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6452..7960)
                     /locus_tag="IWI39_000188"
     CDS             complement(6452..7960)
                     /locus_tag="IWI39_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9207325.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7926..8618)
                     /locus_tag="IWI39_000189"
     CDS             complement(7926..8618)
                     /locus_tag="IWI39_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9207326.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8640..9614)
                     /locus_tag="IWI39_000190"
     CDS             complement(8640..9614)
                     /locus_tag="IWI39_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207327.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9914..10786
                     /locus_tag="IWI39_000191"
     CDS             9914..10786
                     /locus_tag="IWI39_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207328.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11244..13442)
                     /locus_tag="IWI39_000192"
     CDS             complement(11244..13442)
                     /locus_tag="IWI39_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9207329.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13674..14666
                     /locus_tag="IWI39_000193"
     CDS             13674..14666
                     /locus_tag="IWI39_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207330.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14726..16147
                     /locus_tag="IWI39_000194"
     CDS             14726..16147
                     /locus_tag="IWI39_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9207331.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16140..17639
                     /locus_tag="IWI39_000195"
     CDS             16140..17639
                     /locus_tag="IWI39_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9207332.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17678..19072
                     /gene="uxaC"
                     /locus_tag="IWI39_000196"
     CDS             17678..19072
                     /gene="uxaC"
                     /locus_tag="IWI39_000196"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9207333.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19231..19914
                     /locus_tag="IWI39_000197"
     CDS             19231..19914
                     /locus_tag="IWI39_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9207334.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19916..20752
                     /locus_tag="IWI39_000198"
     CDS             19916..20752
                     /locus_tag="IWI39_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9207335.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20763..22517
                     /locus_tag="IWI39_000199"
     CDS             20763..22517
                     /locus_tag="IWI39_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207336.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22793..23134)
                     /locus_tag="IWI39_000200"
     CDS             complement(22793..23134)
                     /locus_tag="IWI39_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9207337.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23202..25373)
                     /locus_tag="IWI39_000201"
     CDS             complement(23202..25373)
                     /locus_tag="IWI39_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9207338.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25584..26441
                     /locus_tag="IWI39_000202"
     CDS             25584..26441
                     /locus_tag="IWI39_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9207339.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26512..27369)
                     /locus_tag="IWI39_000203"
     CDS             complement(26512..27369)
                     /locus_tag="IWI39_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9207340.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27354..30056)
                     /locus_tag="IWI39_000204"
     CDS             complement(27354..30056)
                     /locus_tag="IWI39_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9207341.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(30069..31358)
                     /locus_tag="IWI39_000205"
     CDS             complement(30069..31358)
                     /locus_tag="IWI39_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9207342.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31508..32554
                     /locus_tag="IWI39_000206"
     CDS             31508..32554
                     /locus_tag="IWI39_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9207343.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32556..34709
                     /locus_tag="IWI39_000207"
     CDS             32556..34709
                     /locus_tag="IWI39_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9207344.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35182..36633)
                     /locus_tag="IWI39_000208"
     CDS             complement(35182..36633)
                     /locus_tag="IWI39_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9207345.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITGFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36630..38354)
                     /locus_tag="IWI39_000209"
     CDS             complement(36630..38354)
                     /locus_tag="IWI39_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9207346.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38347..38961)
                     /locus_tag="IWI39_000210"
     CDS             complement(38347..38961)
                     /locus_tag="IWI39_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9207347.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39306..40763)
                     /locus_tag="IWI39_000211"
     CDS             complement(39306..40763)
                     /locus_tag="IWI39_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207348.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40804..41724)
                     /locus_tag="IWI39_000212"
     CDS             complement(40804..41724)
                     /locus_tag="IWI39_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207349.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41736..42683)
                     /locus_tag="IWI39_000213"
     CDS             complement(41736..42683)
                     /locus_tag="IWI39_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207350.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(43162..43881)
                     /locus_tag="IWI39_000214"
     CDS             complement(43162..43881)
                     /locus_tag="IWI39_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9207351.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43930..45576)
                     /locus_tag="IWI39_000215"
     CDS             complement(43930..45576)
                     /locus_tag="IWI39_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9207352.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45755..46345
                     /locus_tag="IWI39_000216"
     CDS             45755..46345
                     /locus_tag="IWI39_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9207353.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46437..47942
                     /locus_tag="IWI39_000217"
     CDS             46437..47942
                     /locus_tag="IWI39_000217"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9207354.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(48026..49378)
                     /gene="celB"
                     /locus_tag="IWI39_000218"
     CDS             complement(48026..49378)
                     /gene="celB"
                     /locus_tag="IWI39_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9207355.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49378..49896)
                     /locus_tag="IWI39_000219"
     CDS             complement(49378..49896)
                     /locus_tag="IWI39_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207356.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49912..50238)
                     /locus_tag="IWI39_000220"
     CDS             complement(49912..50238)
                     /locus_tag="IWI39_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9207357.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50251..52230)
                     /locus_tag="IWI39_000221"
     CDS             complement(50251..52230)
                     /locus_tag="IWI39_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9207358.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52251..52565)
                     /locus_tag="IWI39_000222"
     CDS             complement(52251..52565)
                     /locus_tag="IWI39_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9207359.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52733..53026
                     /locus_tag="IWI39_000223"
     CDS             52733..53026
                     /locus_tag="IWI39_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9207360.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVECFTDLP"
     gene            complement(53104..54216)
                     /locus_tag="IWI39_000224"
     CDS             complement(53104..54216)
                     /locus_tag="IWI39_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9207361.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54369..54593
                     /locus_tag="IWI39_000225"
     CDS             54369..54593
                     /locus_tag="IWI39_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207362.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            54603..54782
                     /locus_tag="IWI39_000226"
     CDS             54603..54782
                     /locus_tag="IWI39_000226"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207363.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            54976..55146
                     /locus_tag="IWI39_000227"
     CDS             54976..55146
                     /locus_tag="IWI39_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9207364.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(55570..56310)
                     /locus_tag="IWI39_000228"
     CDS             complement(55570..56310)
                     /locus_tag="IWI39_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9207365.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        16827 a        11523 c         9919 g        18108 t
ORIGIN      
        1 cacaatgttg gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa
       61 acattgatgc ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt
      121 cactaactag tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga
      181 tcttttccat catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga
      241 tcatgcactg ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt
      301 ttttgcggac ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct
      361 gtctctttct ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa
      421 tcttctattt gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc
      481 tcattccaag taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca
      541 gccatctgat taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa
      601 tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa
      661 atcagacgta aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg
      721 cgtctggcat tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct
      781 ttttcttgtt gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg
      841 ctatctttaa gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct
      901 cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt
      961 cctgacgggg tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg
     1021 tcactagctg tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt
     1081 gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa
     1141 taatacccgt tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca
     1201 aatattttat agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg
     1261 ataccgctgg atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat
     1321 ttttttcccg tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa
     1381 attggttcgg accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct
     1441 ttaaacggac ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca
     1501 taactgattt gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga
     1561 gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg
     1621 attcctggaa gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat
     1681 atattaggat ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg
     1741 attcaggctc cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa
     1801 aaaaatgcaa aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata
     1861 aaatacaaac cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact
     1921 gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat
     1981 aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt
     2041 tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc
     2101 aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa
     2161 ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat
     2221 tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag
     2281 ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc
     2341 acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa
     2401 aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt
     2461 tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga
     2521 aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg
     2581 cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc
     2641 ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat
     2701 tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa
     2761 gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta
     2821 actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc
     2881 ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt
     2941 cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt
     3001 cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt
     3061 ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt
     3121 ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg
     3181 atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc
     3241 gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt
     3301 tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg
     3361 atgtagcaaa agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg
     3421 agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc
     3481 gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc
     3541 ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct
     3601 ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc
     3661 cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa
     3721 ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa
     3781 cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta
     3841 caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact
     3901 aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt
     3961 tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga
     4021 tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt
     4081 taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc
     4141 ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt
     4201 caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat
     4261 ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat
     4321 ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat
     4381 aataaagatt cagtataaat ggacacatca cattatccat gactatattg gatactcgaa
     4441 tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt
     4501 cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta
     4561 atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag
     4621 cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt
     4681 caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat
     4741 ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa
     4801 tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat
     4861 gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc
     4921 taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga
     4981 tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc
     5041 aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg
     5101 cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg
     5161 ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg
     5221 cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa
     5281 tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat
     5341 aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc
     5401 tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc
     5461 atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag
     5521 tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag
     5581 ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc
     5641 ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc
     5701 tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga
     5761 tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt
     5821 ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat
     5881 ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc
     5941 agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc
     6001 tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag
     6061 ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt
     6121 tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc
     6181 ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt
     6241 tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag accattttcc
     6301 tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt
     6361 ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa
     6421 taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct
     6481 cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga
     6541 atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt
     6601 caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta
     6661 cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat
     6721 ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt
     6781 gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc
     6841 cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt
     6901 gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc
     6961 cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct
     7021 tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct
     7081 tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat
     7141 cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg
     7201 aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat
     7261 cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat
     7321 aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat
     7381 caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa
     7441 catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt
     7501 ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt
     7561 cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc
     7621 ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga
     7681 tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag
     7741 gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt
     7801 ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt
     7861 ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg
     7921 caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt
     7981 ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg
     8041 caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag
     8101 gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc
     8161 aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa
     8221 aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca
     8281 tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga
     8341 tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga
     8401 tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc
     8461 agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc
     8521 aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga
     8581 tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct
     8641 tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac
     8701 aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc
     8761 aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc
     8821 aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc
     8881 aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt
     8941 cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa
     9001 aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg
     9061 cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat
     9121 tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc
     9181 caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc
     9241 gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt
     9301 aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa
     9361 cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa
     9421 tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg
     9481 actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt
     9541 tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc
     9601 tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg
     9661 atagataact atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat
     9721 agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc
     9781 atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta
     9841 atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga
     9901 ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa
     9961 caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc
    10021 tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac
    10081 cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc
    10141 tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat
    10201 ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt
    10261 attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg
    10321 gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat
    10381 cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga
    10441 tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat
    10501 tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt
    10561 gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt
    10621 atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt
    10681 taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca
    10741 gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg
    10801 caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa
    10861 ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca
    10921 ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca
    10981 aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca
    11041 tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta
    11101 aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt
    11161 aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc
    11221 cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc
    11281 gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct
    11341 gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga
    11401 tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa
    11461 tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat
    11521 tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt
    11581 gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct
    11641 ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt
    11701 gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact
    11761 acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact
    11821 aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct
    11881 atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata
    11941 attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat
    12001 tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc
    12061 aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct
    12121 tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc
    12181 ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt
    12241 agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta
    12301 taatagcagt cattttgaga actataacca aattgctgca accgctgatc tagttctcgc
    12361 ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg
    12421 gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg
    12481 accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt
    12541 tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct
    12601 accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt
    12661 gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt cagtactata
    12721 tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt
    12781 aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg
    12841 ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc
    12901 tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc
    12961 atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc
    13021 tgttgatact tttcaacttc ctcttcggtc agttgactca acgatccatt gattactccc
    13081 catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata
    13141 aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc
    13201 ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt
    13261 ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa
    13321 taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca
    13381 agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc
    13441 attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact
    13501 catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca
    13561 agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat
    13621 cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa
    13681 cgatcaaaga tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga
    13741 ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa
    13801 tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg
    13861 gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg
    13921 caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg
    13981 ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg
    14041 atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca
    14101 atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta
    14161 tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa
    14221 agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag
    14281 cgagcatccc tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc
    14341 tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca
    14401 acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc
    14461 agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc
    14521 ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag
    14581 atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc
    14641 ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg
    14701 tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa
    14761 cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga
    14821 ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca
    14881 acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat
    14941 tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga
    15001 acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga
    15061 cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa
    15121 tttaacagat actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg
    15181 tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg
    15241 cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt
    15301 tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc
    15361 aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt
    15421 gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa
    15481 tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta
    15541 tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt
    15601 gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt
    15661 tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc
    15721 catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga
    15781 agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa
    15841 gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact
    15901 tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt
    15961 tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct
    16021 ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc
    16081 aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca
    16141 tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat
    16201 taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg
    16261 aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga
    16321 tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc
    16381 acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg
    16441 tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg
    16501 gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca
    16561 ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc
    16621 atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata
    16681 ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg
    16741 gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg
    16801 aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg
    16861 ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct
    16921 ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta
    16981 atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa
    17041 cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag
    17101 aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg
    17161 gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag
    17221 aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac
    17281 aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc
    17341 ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa
    17401 gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt
    17461 ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg
    17521 aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga
    17581 aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat
    17641 tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt
    17701 attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat
    17761 tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct
    17821 gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt
    17881 gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca
    17941 aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg
    18001 tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc
    18061 aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt
    18121 agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc
    18181 tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat
    18241 tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat
    18301 caccacctat caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca
    18361 tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac
    18421 agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat
    18481 cgatgcttat cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg
    18541 gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt
    18601 agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa
    18661 attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa
    18721 tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg
    18781 aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca
    18841 attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc
    18901 aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt
    18961 aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt
    19021 tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa
    19081 tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt
    19141 ttagagaaga agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg
    19201 cctataaacc tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct
    19261 actgcggcag aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct
    19321 gcttattttt cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa
    19381 tcatttatcc aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc
    19441 ttatttgtac aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc
    19501 ttcggtagct atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc
    19561 caaatggtct tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat
    19621 ccagatatac ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt
    19681 cctcttttag atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag
    19741 acaaaaaaat tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta
    19801 aaagacaata cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa
    19861 ttgataaaag aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt
    19921 tccattaaat ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt
    19981 tcaaaaagta tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc
    20041 tgaacaactt aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt
    20101 ccaaaaaaac aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa
    20161 tcaagaagtt cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc
    20221 ctatcaagcc aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga
    20281 aaattttact gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca
    20341 tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa
    20401 ctatcagtta gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt
    20461 agattgtgcg aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg
    20521 tgccttggac aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga
    20581 aaatggaaag atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact
    20641 gcattatatc aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat
    20701 ttatgttcct gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga
    20761 ttatggagga acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat
    20821 tgctgcatct ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta
    20881 tcaaacatct tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt
    20941 catcgaaaga ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc
    21001 tgattggtca aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc
    21061 gtcaagtaga agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc
    21121 cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag
    21181 ctatccaaac gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat
    21241 tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga
    21301 ctgttcctct cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact
    21361 cagacttcag gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag
    21421 caccgattgc tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt
    21481 ttactcaaat ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag
    21541 tcagctgggc tagttttcag atatttcagc tgatttgttt gattttcact gggatattag
    21601 ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga
    21661 tcgtcaattc cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat
    21721 ctatcacttc tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga
    21781 aaaaaatcca acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg
    21841 actcactcaa accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg
    21901 cttttgttgg gccatctggc tctggaaaat ctacactcct taacttattg attggattta
    21961 tccagccgac ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc
    22021 gaagtgtgcg aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga
    22081 tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca
    22141 tcgaagctgc gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag
    22201 taggcgaaca tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag
    22261 cgttgatacg tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc
    22321 agtcagagaa aaaaatccag caagccctga attacctgac agaaacaccc actactttta
    22381 ttgtggctca tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag
    22441 gaaaaatcgt tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc
    22501 gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat
    22561 ttctgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa
    22621 catgagaagc tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg
    22681 acctcttggt aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat
    22741 cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc
    22801 tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga
    22861 agccttgctc tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca
    22921 tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt
    22981 gtaattcgat ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt
    23041 gtactttctc gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt
    23101 cgatttgatt cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt
    23161 tactctctat tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc
    23221 cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg
    23281 aagtttttca gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt
    23341 ccagactctc cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct
    23401 cctgtggcat aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct
    23461 tctgccgctg atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc
    23521 agcttgctgg attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg
    23581 atcatccatt ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc
    23641 cctgctatcg tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca
    23701 agaagcatgc tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg
    23761 tttgtgttat cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc
    23821 gtatacatct gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat
    23881 actccttttt ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc
    23941 aggccgaatt cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat
    24001 acagtaggca aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat
    24061 gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa
    24121 ggcttgtatc cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct
    24181 ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt
    24241 cctactagtg cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc
    24301 atggcaccat ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat
    24361 tgcttttgat aatcctgatc taaagctgta tagattttat agcctttatt catgacatct
    24421 tcttcatcaa gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga
    24481 taacgataac cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc
    24541 tggctttctg cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt
    24601 actgtgtttt tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga
    24661 cctttcagca ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact
    24721 ccaaaatact ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg
    24781 ttaagataca tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa
    24841 aataattcct tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca
    24901 agctgctgag tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg
    24961 actgctgcac gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct
    25021 tctgtcgaga ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt
    25081 cctttttggg cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat
    25141 tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag
    25201 atacttgtca ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat
    25261 tttttccaga tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc
    25321 caaaaactga tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc
    25381 ttctcctgtc tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc
    25441 tgcttaagtt gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat
    25501 gaacagaaaa atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg
    25561 attgcactga aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg
    25621 aaacaacaat tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact
    25681 ttttgcgtat acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag
    25741 taaagggcaa tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag
    25801 ttcaattagg agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag
    25861 tggataagcc tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac
    25921 tcaatcattt agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct
    25981 tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac
    26041 ttggaagaat gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag
    26101 aaattgccaa agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa
    26161 aaagggtcgt agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag
    26221 tactttctga acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca
    26281 gagttcattt agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa
    26341 cagctgaacg actgatgctc catgcattag aactgcacat gatccatcca tttactcaag
    26401 aaaaaatagt cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt
    26461 cttatcagca gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg
    26521 atgttcgtgt tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca
    26581 acgaacgata tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa
    26641 ttgctgacgg attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat
    26701 cttttcaggt gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg
    26761 aatagcccaa acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc
    26821 aatagacggt cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc
    26881 ataatggtca tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc
    26941 tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta
    27001 aatacaacta ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt
    27061 gacatccgtt gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc
    27121 gaattgtgcc cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt
    27181 gtcgatacca atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa
    27241 aaattcaaat acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttttc
    27301 taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc
    27361 gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt
    27421 gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg
    27481 tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact
    27541 ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt
    27601 aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa
    27661 ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt
    27721 tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat
    27781 ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct
    27841 gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga
    27901 atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca
    27961 tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac
    28021 tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca
    28081 aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt
    28141 ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt
    28201 tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct
    28261 atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt
    28321 ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca
    28381 aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca
    28441 tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc
    28501 attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa
    28561 aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca
    28621 cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct
    28681 ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac
    28741 tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt
    28801 atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg
    28861 tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca
    28921 agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa
    28981 tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca
    29041 taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt
    29101 tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga
    29161 gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct
    29221 tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa
    29281 ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat
    29341 atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag
    29401 gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct
    29461 actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt
    29521 tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa
    29581 acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca
    29641 gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct
    29701 aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg
    29761 ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat
    29821 tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag
    29881 tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca
    29941 aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc
    30001 atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac
    30061 gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc
    30121 atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct
    30181 tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt
    30241 tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt
    30301 tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt
    30361 tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat
    30421 cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt
    30481 cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg
    30541 tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct
    30601 ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac
    30661 atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg gaaaccagac
    30721 catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct
    30781 tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg
    30841 aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg atcagttttt
    30901 cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca
    30961 tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg
    31021 ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt
    31081 ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa
    31141 ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa
    31201 aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct
    31261 gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata
    31321 aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt ctttctattt
    31381 gatatatctt taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat
    31441 ctttttattt actttttcat ccattatggt attatttgaa tatgatatat cattaagagg
    31501 tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt
    31561 aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta
    31621 taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat
    31681 ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc
    31741 ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt
    31801 tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat
    31861 gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg
    31921 attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt
    31981 gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt
    32041 tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca
    32101 ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta
    32161 ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga
    32221 caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt
    32281 tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta
    32341 tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt
    32401 tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga
    32461 tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc
    32521 attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta
    32581 gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg
    32641 ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt
    32701 ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga
    32761 tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc
    32821 ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt
    32881 cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa
    32941 tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc
    33001 atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt
    33061 cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg
    33121 atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa
    33181 aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc
    33241 tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc
    33301 agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt
    33361 atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg
    33421 ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa
    33481 ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag
    33541 aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc
    33601 caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca
    33661 ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa
    33721 tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag
    33781 attacttgac atatggctat gttcctaatc actaccacga atcagtcaat cacacattag
    33841 actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa
    33901 tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag
    33961 gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt
    34021 ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt
    34081 tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctcattgagc
    34141 tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga
    34201 gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact
    34261 atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt
    34321 tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa
    34381 gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg
    34441 gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa
    34501 aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca
    34561 aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg
    34621 gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt
    34681 tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg tcctgcatca
    34741 agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt tggtcttatt
    34801 accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca gcgttctgac
    34861 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta
    34921 ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa agctgtttag
    34981 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
    35041 ttaataccga gaaactggct tttgaactcc gtttatcgag aggtcatgaa aaagctgcct
    35101 tgcctcaagt aataagaaca gcccaaaatt agatttctag acaatttttt ataagttatt
    35161 tcattacatt ggctttttct tttatgattc tccactgttt tctcggtact ctttagggga
    35221 tctgccgacg attttcttga ataatttaga gaaatacgtg gtgttgttgt agccaatctc
    35281 acttgctatc tcattgatgt tttgattact gtgcaaaagc agttgtttcg ctttttccat
    35341 acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt tcttttttga acagctgtcc
    35401 tagatacatg acatttaaat gcaggttttc tgacacttct ttgagtgtaa gtggttcttg
    35461 ataatgctgg tgcaggatta cgattacttt ttccacattg tcactgtact ttttctgtct
    35521 ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt tcttgcaccg tttttgcctg
    35581 atggattttt tcgattcctt gtaaatattc ctcatcttcc agatcaatca attcccgttg
    35641 aatctccata aacagtaaaa aactggaata tcgtatatct tccggcatca tggctgctaa
    35701 ctgaaactgt tcaaaaaaac cagtgatcat tttttgagcg acttccaatt gtttattttg
    35761 cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct tttgtttctg cttgtactgc
    35821 gtaaaaaaca tgctcgtctt tatctccata aaattgatgc aactgtagat tgtctttcgc
    35881 ttgttggaat gtttcaggga ttttgtccaa agaatctgtc tcttccccga tactgatcag
    35941 ccattcctga tcaggaaagt tttcttgaac aaaacaacag aagccatcaa cttcatctgc
    36001 ctccaacaac aacgtgatca atacacggtc tccgtaattt cgttgatagt aaaagatttg
    36061 ttgccgttct tgcaaccatc tattgataag agtatcatga gttcttgcca gttgaatcaa
    36121 tactatccta tgctgcttct cacttatttg tcctattatt tcttcttcac ttatttcatc
    36181 caattcttca ttcaaccatt gactgaataa cagttcttga tagatttctt gttgattttt
    36241 ttgttcattt tgctgatcta accgtgtttt gattttcttg aggctttcaa ccaattcgaa
    36301 cttattcacg ggtttcatca aataattcac ggcgcctaat tgcatacctc ctttcaaata
    36361 atcgaattct tggtatcctg ataaaatcat aaattcaaaa ttatgacgtt ctttttgagc
    36421 cgcttcaata aattccagtc catttatttc tggcattgtc acatccgtta ctactaaatc
    36481 aatctgtcgc tgttcaagta cagctagtcc ttctttggca ttctctgctg ttgctactat
    36541 ttgaaagcct aaactttgcc aatcaattat cttttttaac ccattcaaaa tcatgtattc
    36601 atcatctacc aataacactt gatacacgct catccctcct tgaatgtaat agaaacggtc
    36661 agtccacctg tctcattatt ctcaagcgac atataataac tgctgccgaa gctggcacgt
    36721 aaacgttcat tcacgttttg cagaccgata gaaccgtgaa gttccacttt aggatgagac
    36781 agtttttctt caatctgttt tagccgtttt tcagtcatcc cttttccatt atctttgatg
    36841 atgatcctta cccgtttgcc ttcctgcaag actttgacac ttagcgcatt atcgaatcga
    36901 gtaaaatcaa taccgtgttt aaaataattt tcaactaatg gctggatcac aaatttaggt
    36961 acttcgatct tttccaaatc aggatcgatc ataaaatgat aggctactcg attaggataa
    37021 cgcatttgat acaaatagac atatttttcg caaaagtcca gttcttcttt taaagtgatc
    37081 gttttctctt gattcgtatt gtttcgaagc aaagcggaaa acgcatacac aacatctgct
    37141 aattcttcac ttccttcgct gattgcatac attcggatat attctaatgt attatataaa
    37201 aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg cgtcttgctg tttgatttct
    37261 aatttgtaga tgtcttctac aaactgattg atgctatcca acatttcgtt gataccgatt
    37321 gaaagttcct tcagttcata ttccgtgttt gttatgtcga tccgcgtctc taaattccct
    37381 gctgctgttt gatccatggc ctgcatgacg atagatactt gctgtgaata tcgtttgaat
    37441 gttttataaa gaaagataag cagtatattg tctaaaataa tgataccaat gatcagcggg
    37501 gcaagatttt ttcccgtttc gatttttaca ctttttttat ctagtacagc taatatacga
    37561 taatcatcag acagttcttg gtgttgaaca aaattgtttt tttccagatc gtcaatcggc
    37621 aattgtgaat ttttcttcag actctctgtg atgatcattt cttgtctttc aaacgtttct
    37681 ttagctattt tattatcatg gaatgtgtat agtcgatttg tcgttccaga aatcatgtat
    37741 aaagagagtc cattgaatga cgttagattt tctaaaatca tatctagttc tttcttttga
    37801 aaaccaataa aaatacttcc tgactcagct cctccttgtg tgataggcaa cttgatataa
    37861 aaggcgtcta gtaattttgg tgtccccttt tttatttccc cacctttgtt ttctctagaa
    37921 gattcataat actccggtag ttgattcaac acgatatata tcgcacttag ttcttcataa
    37981 tttgcgtaga gatctttgat ttgatttggg aaagaaacta attcatcggt actgttttgc
    38041 tgttcgtata catacatcag atactgatct atgggttttg ttaagtaggt cgtcacattt
    38101 tcgatctttt cggaattccc tacaagctgg tttttgatga tcctactcaa ggttgtttta
    38161 tcattcagca ttctgcttgt gctttgaacg gcatcacttg tcatgttttc tgcctgtttt
    38221 tgattttgtt cccaagtatg gatactaaaa ataacggtag cagtcatcgt tacaccgatc
    38281 atgataaccg cgtatttccg caacagacga tttagtaagg ttccttttga aaatagaatc
    38341 cgaaacttat tcataacttt ccaactgttt atctaattct tcacgccaat gtgttgtcat
    38401 cgtaccattc caaaccgtca aaagaccaat gacaccaaac aggatcaatc ctttgaattg
    38461 ccaagtcacg cctaaaatta taccgctacc aataattatt tttagaaaag acgaaaagct
    38521 ggaaaaaacc gaaataaaac ttagctttag cagattagga acagagatcg tatattcact
    38581 atctaaaatc atactgtatt gataagtagc aaataggcag agcaaggcaa aaagtaaaat
    38641 aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca gataaatata aattgtaact
    38701 caataaagca gtaccagata aaaataaact aaatagcaga ttgcctctta aaaaatttct
    38761 tttatatata ttccagcctc tttttattgt gatttcttta tactcgaatc cgtacaaata
    38821 aaatagttca ttgactgttt tccatgccgc acctaatcca aagatgatcc cgccggcaaa
    38881 tgtatatatc cagaaataca aggttagttt tataatgacc cagatccgga taaataactc
    38941 ttccaaaacc ttccctaaca tatcttctcc tccaaacatt aagtttatac tttctcatta
    39001 tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta ttagagaaac aaacggaaaa
    39061 atagttcctc atattttgtc gttttgtgag cgaataccga gggttagctg attgaacacc
    39121 gtttaccaag agtttgtgaa atagccgctt agtttcgagt agtaagaaaa attttgtgaa
    39181 tatggctttc cacattttta catatattta gcttaatacc gagaaactgg cttttgaaca
    39241 ccgtttattc ggaacaagag gttgtgacac gtcttttgtc acaacccctt tgtctttatt
    39301 cattattatt cttgtcggaa ctcatcatat tgtttttgca tttctttcaa taccttatca
    39361 tagccagcct tgtccaaagc gtctttcaat ttcggtaatg tttcatctgg atcaacagtc
    39421 cctgtattca atccatctaa atattgattc atgacattag aaatattact taattctgtt
    39481 ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg aagtttctgc atcagcaatc
    39541 gattgatcac gtttggcaat catgtcatcc gtaatagact cttgtgtata tagaatcttg
    39601 ttgtttcctg tattccaagc agacatatgt gtatttggct gataaccatc tagcaatttg
    39661 attttatcat ctgaaccatc gactttttcc catgcttctc cctcgatccc ccaaacaagc
    39721 ccattcaata attctggatc actgttaagc agccctagaa gttctactga tttttcttta
    39781 ttttttgaag tatttgatac gacaaaattt gccatttgtg cttgagctgt agattttagt
    39841 ggaacagtaa ttgcttttga gacaagttct tgttttgctg catttgtcaa aattgtatcg
    39901 ccataatcat acggtccttg tgtttctcca cgcattagcc aagtatttcc ttctagtgga
    39961 taatccttat tgctcgttgc agcgtcactt ggaatgagtc cttgtttgta ccagctatgc
    40021 attgtacgta acagtccgat atacgtttca ttatcgtatt ggttgatgat tttcgaatca
    40081 tctccatcca gatcaatagc aaatggtaac gtattcccta aaggataatc aaagttccct
    40141 tggactttga agccttgtcc aatggcaaaa gctgccgtgt ttggttcttt ttcatggaat
    40201 tccttcaaga ctttttctgc atcagcgtaa gattggattc catcaattga aagatcgtat
    40261 ttatctagta aagctttgtt aaaagtaagt acttgttgag caaagacatt tccattgact
    40321 gggaaagcat ataattttcc attgaccaga tttcctttaa tatatgcttc atctaaatca
    40381 ttataagctt tttcagcgta cttaggcgct aattcagtca aatcagcaaa ggctcctttt
    40441 tgggcattgg aaacatagtt gttcgcaaag gcgatatcat aattttcccc ggaagaaatg
    40501 atgacgttca ttttcttttc gtaatcgccc cagccaatgt aattgatatt gacttttaca
    40561 ccgatctctt tttcgattcg tttgtttgct acttccatca attgatcgaa attatctggt
    40621 ttatctccga tttgatacat ctgcaaagtg tccgaattgc tagcttctgc ctggtcttta
    40681 gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca acgctgaaac acctgtaaag
    40741 gctagtttct tccataactt catttttctt cctcctaatt ttatgattta tatttttgtt
    40801 tctttattct tttactccgc cgattgttag acctttgaca aaatgcttct ggaaaaatgg
    40861 atagctgatc gcaataggca acgtcgagat gactacgatc gccattctgg cagattctcc
    40921 tggaactgag gctaatccgc ctgtcaattg gcttcctgct cctgaatttt gtgtcagata
    40981 ttgaatatta ctttggattt tcatcaaaag atactgtagt ggaactagct tatcatcttg
    41041 gatgtatagt aaagcattga accagtcatt ccagtaaccc aatgcggcaa ataaactgat
    41101 tgtcgcaata cctggaacag ctaacggtaa aacgatctga acaaatattc gcagttcgct
    41161 agctccatcg atccgtgccg attcaataat cgaatcagag accgaacgtt taaagaatgt
    41221 gcgcatcaca ataatattga agggactgac agccattggc aagatcaatg cccaaatcgt
    41281 atcttttaac tgaagaagat ttgtcataac aagataattg gccaccatcc ctggtgagaa
    41341 caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg aatggaaagt ttgaccgtga
    41401 aatcgcataa gcatataaag aagtagccgt gctattgatc agtgtaccta aaacagtaac
    41461 aagtacagtg acgaaaagcg cttggaaaat tttatcctgc atctggtcga acaagtacgt
    41521 ataaccaaat atactgaact tttctggcca aaaactgtac ccgtgagtcg acaatatgga
    41581 ctcatcggta aaagagatgg cgataacaaa tataaatggc aaaatacagg aaatagcaaa
    41641 gagtgcaatc attatattag agaaaaaatt agccgtctta ttgaaagagc ggacttggac
    41701 tctctccact ttcttttttt tcattttttt cctccttaga ataatgcaga atcgctatca
    41761 aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa agccgacaac tgattggtaa
    41821 agtcctgcag cagccgtcat accaaaatct cctgtggatg tcaggccatt gtagatatac
    41881 gtatccaaaa cttgagtcac atcatataat gcaccggaat tcctaggaat gttataaaac
    41941 aggccaaaat cagctcggaa aatgtttcct acggctaaaa tcgttaaaat cgtcattagt
    42001 ggaataagtt gtggtatcgt cacatgtttg atctgctgcc atttgctagc tccatcaacc
    42061 atagcagctt cataataagt cggatcgatt cccataacag atgcaaagta aataatgctg
    42121 ttataaccaa tccctttcca tgtaccaatg aacaccaaga taaacggcca atatttcggt
    42181 tcattatacc aattgacagc ttctcctcca tgtccagtga tccactgatt gaatattcct
    42241 ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa tgacccaaga tagaaaataa
    42301 ggtagtaagg acatcgtttg ataaacttta acgagtcgtt tatttctcaa ctcgctcata
    42361 atgattgcaa atacgactga gatcagcaga ttcaaaagga taaatgctac attatataaa
    42421 attgtatttt tagtgatgag aaatgcttga tccgaagaaa ataaaaattt gaaattttcc
    42481 caacccaccc aaggactttc cctcaagcta gccaagaacc catctgctga aatatgaaaa
    42541 tttttgaatg ctacgacatt cgccattaca ggtatgtaaa agaaaaaaat catccaaagc
    42601 atacccggta cagccatcag gacaagtgct ttataacgcc atagattcat aaaaaaatga
    42661 ttgaacccct ttcgtttttt catctaaaaa cccctttcga ttcattcgca acgtaattat
    42721 agcgctttca ataatgagaa taaataaaca aagtataggg aaaatgaaac aaagtatagg
    42781 agaggttgtg aaatcagcgt tttggcctga gtattagaga aaaaaacggg aaaatagttc
    42841 ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc
    42901 agaggttgtg aattcagcgt tttggcctga gtattaggga gatcgtgtcc ttactatcta
    42961 actctgagtc acaaggaaca atggggactg taacaaaagt cttgtcacag tcccttattc
    43021 cgaacaaacg gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaaag
    43081 ccatccgaaa gtcagacgga caagtctgct tttcagatgg cgctacaact actctgctta
    43141 ttcttttagc tatccattca attatttttc tctcacttgt tctagctctt ctttggtcat
    43201 gggtcgatat tgccctttat tcaaatcttt aggcagccaa aattttccca tacggattct
    43261 ttttaaatat aagacttctt ttcctactgc tttgaccatt cgcttaactt ggtgaaactt
    43321 tccttcatgc aaaatcagac ggataattga agtttctgtc tcttgatcta cttgatcaat
    43381 ttggagtttt gccggcaatg tctgctcatc gccatcaata atcagtcctt ctgcaaataa
    43441 acggatatct tcttctgtca ttataccgct aacctcagct agatattctt tctccacatg
    43501 ttttttaggt gatagtaact gatgggcaaa caccccgtca ttcgttaaaa tcaagaggcc
    43561 ctctgtgtct ttatctaatc ggccgactgg aaaaagatct tcccgataat cctggtcttc
    43621 caataaatcg attacggttt gatcatgatt atcctgagta gcagaaatga ctcctgcagg
    43681 tttatgcaaa atgtagtaaa aatctttttg atacgtaatg atttctccta aataagtaac
    43741 cgcatcttcg aattcattta cttgcgcttt atcgcttttc accacttgtc cattgacttc
    43801 gatcgttcct tttttgatta atcctttgac ttctttgcgg ctgccaagtc ccatatctgc
    43861 taaaaactta tctaaacgca tgcgtttccc ccatttcaaa agagcatcaa tctagctctt
    43921 ctgattcact tattttattt ttaatttatg tcgtaaacgc gctattcctg gtcctagtag
    43981 tctttctgcc aagcggattt tcaaagctaa ataaccatac actaaagccc ctatacctgc
    44041 tactacaaga atcaacaata gagactggaa tttgctatct tcgtttaaaa aagcaccaaa
    44101 aatcatcttt gtgatccacg aagccaaaag cataatcaag gtaagaataa agatcagcaa
    44161 tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact tcatggattc tctttaagat
    44221 caaggtacaa gacacagcaa atccgatcat tgtcgccaat aaagggccgt atacttcaaa
    44281 taaacgaatg gctgggtatt gaagaaggag tttcactaga aacccaataa ccaaataatt
    44341 aattgccgcc ttgttttcaa acattccttg cagcatgttg gaaactagca tatatagtcc
    44401 taggaagagt cctacaaaac ttgcttggat cagtacatta cttcctagag aatccggtgt
    44461 ataaaataat gtatttagcg gataggcaag taacataaca ccaaatgtcg ccggaaacat
    44521 aaaaaatgaa aacaactgta aatttccgct tgtcaatttt gccagaccta ctcgatcttt
    44581 caacgtaaca gcttctgtaa tcaacggtaa cccagtagac gcgatcgatg ttgccaatgc
    44641 aataactacc atcgtcaact tatctggatt ggcactaaaa atcgaaaaaa gatccagtaa
    44701 ctgtgcattg gaatactcgg tggatgcaga catgatcttc ataaaagtga actgatcgac
    44761 taatttgaag atcgttaccc ctgaacccac aataataaac ggaatcgctt ctttaacggt
    44821 atctaacaac aattccttcg ttgcaatatt tattttgttt tcgctgtagt gaataaacgc
    44881 cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg acaagaatac ttgctagcat
    44941 accgataaat gccgcgaaag ttgattgcgt gaccgccgtc acatagtcac catccatgac
    45001 tttcatgatg atgaatgtag ccaatagcat ataaaaaata cgtgccacct gttctacaat
    45061 ctgcgacagc gcataaggca tcatttcttg attgccttgc aaataccctc gtatcacact
    45121 catacaagga aaaaccaata cagcgatact caaagaacgc atcgttggaa tcagttcttc
    45181 tccgccacca gaagcatgag ctagccaagg agaggcgatg tacatgaaca aagcaaacaa
    45241 caggcctaac ccgcccatca tctgtaatgc acggataaat aatcgtcgtg aagtgccata
    45301 ttcattcaat gaattatagc gtgcagtttg ttttgcaatc gcagcaggta ttcctgctgt
    45361 agagatcagt agaaacaatg catatatatt gtatcccata ttgaataatc cattggcagc
    45421 cttagcattt tcgcccatcc aagcatacca gggaatgata tagacagcgc ctagcaatct
    45481 tgaaatgata ttactggcgg tcatccaagc agagcctcgt gccatttttt cttgcggtgt
    45541 taattgatct aatgctggtt ttctttgatt atccatcggt cagattccaa ctttctacaa
    45601 aacattacta tcattttaat tggtaaaaca gaaactggca atcgatttta tataactttt
    45661 ataagaacaa ttgttttttt gaattcggtt tcgttttttt ggtaaagtaa agaaatatac
    45721 agccttttat ggctaacatt tataggagat atcaatgaca caagaaataa aaaaactacc
    45781 cgttatattt ttaggtcttt tctttatcac agttggattg aattggttct tgctccctca
    45841 cgatatagct tccgccggag tgggcgccat cggacattta gttgaaacgg ccttttctat
    45901 ccaacgctca gttactgttt ggagcatcaa tcttatgatg ctgcttctag ccgctgtcct
    45961 attaggaaaa tcagtatttt ttaagactgt agcgggaagt ctgctttttc ctgttgtttt
    46021 agagattgtt ccaaaagtgg aaatgatctc ctcacatttc ttttcactga tttcaggaag
    46081 tcttttgttc agtcttgggg tttacacatt gtacacagtc ggggcctcaa atggcggtat
    46141 aacgataccg ccgattattt tccacaaatt ttttcggctt ccaatgccgc aaggactttt
    46201 acttaccaat agtcttatcg tctttttgaa ttattatgtt tttggattgt tggagacact
    46261 attcgttctc ctatctatta cagtcagttc attgtttatg aaaatactca tccgcttgtc
    46321 ccctgtgtca agaaaatttg aataaagctg gattttgtca tagcggaaac aaacgatata
    46381 ttaaaaagag tgtgctataa taaaccaaga aattgaataa agcgagggat caatcaatga
    46441 gcttctcttt agaagacatc cgtcatttac tattaaaaga aaatctatta aaagaatttg
    46501 tttcaacaag aggctggcat cttgatgtac cagaaaaagc tgaatttctt cagttaagtt
    46561 atgattcaag aaaagctgat gcttctactt tgtttttctg taaaggaatg aatttcaaag
    46621 aagaatattt ggattcagca atcgaacagg gtattcaata ttatgtatca gaacaaccat
    46681 acgaaaattc agcagtagga atcatcgtaa cggatatccg taaagcaatg gcgcttcttg
    46741 ctatggcttt ttacgactat ccgcaaaata aattgaaagt gatcggattc actggaacaa
    46801 aaggtaaaac aactgctgcg tactttacaa aagcaatctt ggatcacaca acaaataaaa
    46861 aaacagcatt gctttctact atgaatacaa cgttagatgg caaaacatat ttcaaatccc
    46921 atttgacaac gcctgagtca ttagatttat atcgtatgat gtcagaagca gttgaaaatg
    46981 gtatgactca tctagtgatg gaagtttctt cacaagctta caaaacacaa cgcgtatacg
    47041 gcttgacttt agatgtcgga atcttcttga atatctcgcc tgaccatatc agtccgatcg
    47101 aacatccgac atttgatgac tatttctatt gcaagcgaca attgatcttg aattcaaaaa
    47161 cagttgtttt aaatcatgaa agtgactatt ttgatttact tagggaaaca gccgatctat
    47221 tccagattcc aacgatcact tatggtcgtt cggaagaaag caattatcaa gtgatccgtt
    47281 cagataaagg tactcacggt tttactttat cttctcatga agatcgctta gctatctgcg
    47341 atactgctta tgatattttg ctggctggag gattcaacca agaaaatgca gccagtgcga
    47401 tcattgccgc agcactaaca ggtgcctcca gtcaagatgc acaagacgga ttaaaagaag
    47461 ctcgtgtacc aggccgaatg gatcaattga tccaatccaa tggagcgcat gtctatgtgg
    47521 actatgcaca taattatcta agcttgaaaa cattgctgaa atttgccaaa aatgaacatc
    47581 cagatggccg tgtaattgtt gtattaggca gcccaggaaa caaagcaatc tcccgtcgac
    47641 atgattttgg aaaagtgcta tcagaaacag ctgacatcgc ctttttgacg gcagatgacc
    47701 cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa tgaagcaatt actaatccag
    47761 atttgatcgt tcattatgaa atggatcgtc ctgaagcgat tcgacgcgct ttagcagaaa
    47821 gcgctccgca agattcagtc gttattgcgg gaaaaggcgt ggatccctat caaaaaatca
    47881 atggtgtaga tgaaccttat gaaggggatt atgcaatcgt gaagcggttg attgaagaat
    47941 aatggtattt atatactagt ggtttcccat ttctaagaaa aaaagggact gtgatttttt
    48001 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
    48061 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
    48121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
    48181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
    48241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
    48301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
    48361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
    48421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
    48481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
    48541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
    48601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
    48661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
    48721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
    48781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
    48841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
    48901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
    48961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
    49021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
    49081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
    49141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
    49201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
    49261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
    49321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
    49381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
    49441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
    49501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
    49561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
    49621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
    49681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
    49741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
    49801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
    49861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
    49921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
    49981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
    50041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
    50101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
    50161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
    50221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
    50281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
    50341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
    50401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
    50461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
    50521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
    50581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
    50641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
    50701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
    50761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
    50821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
    50881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
    50941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
    51001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
    51061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
    51121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
    51181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
    51241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
    51301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
    51361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
    51421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
    51481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
    51541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
    51601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
    51661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
    51721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
    51781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
    51841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
    51901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
    51961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
    52021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
    52081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
    52141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
    52201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
    52261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
    52321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
    52381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
    52441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
    52501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
    52561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
    52621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
    52681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
    52741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
    52801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
    52861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
    52921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
    52981 gtataaatta aaaatgaaag tcgaatgttt cactgattta ccgtaaaaag atacacatgg
    53041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
    53101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
    53161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
    53221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
    53281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
    53341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
    53401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
    53461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
    53521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
    53581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
    53641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
    53701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
    53761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
    53821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
    53881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
    53941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
    54001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
    54061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
    54121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
    54181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
    54241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
    54301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
    54361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
    54421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
    54481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
    54541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
    54601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
    54661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
    54721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
    54781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
    54841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
    54901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
    54961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
    55021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
    55081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
    55141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
    55201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
    55261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
    55321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
    55381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
    55441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
    55501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
    55561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
    55621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
    55681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
    55741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
    55801 cgcatccaat tcataccccg ttggcgcttg agtttctacc agctgatatg ctcctggtgc
    55861 taagccatct atcgccagtt ttccatctgc tcctgtggtt agacctgact gcagaacggt
    55921 tccattggca tcttgtaact caaaaacagc tccttgcagg acttctccgc tttggtcatc
    55981 gatctttgtc aacacgacac cgcctggtgt aagtcgattt tctttcgtca attctaccac
    56041 cgctgtctga cttctttcga tctcaaactc gattggtgtc gcatccaatt cataccccgt
    56101 tggcgcttgg gtttctacca gctgatacac tcctggtgct aagccgtcaa tcgccagttt
    56161 tccatctgct cctgtggtta gacctgactg cagaacggtt ccattggcat cttgtaactc
    56221 aaaaacagct ccttgcagga cttccccgct ttggtcatcg atctttgtca acacgacacc
    56281 gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat
    56341 ctcaaactcg atcggtgtcg catctaattc atacccc
//
LOCUS       DACDOA010000005        56306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDOA010000005 DACDOA010000000
VERSION     DACDOA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.005"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            41..429
                     /locus_tag="IWI39_000229"
                     /pseudo
     CDS             41..429
                     /locus_tag="IWI39_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            557..1240
                     /locus_tag="IWI39_000230"
     CDS             557..1240
                     /locus_tag="IWI39_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9207366.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1549..1622
                     /locus_tag="IWI39_000231"
     tRNA            1549..1622
                     /locus_tag="IWI39_000231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1583..1585,aa:Arg,seq:tct)
     gene            1781..2140
                     /locus_tag="IWI39_000232"
     CDS             1781..2140
                     /locus_tag="IWI39_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9207367.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2263..3894
                     /locus_tag="IWI39_000233"
     CDS             2263..3894
                     /locus_tag="IWI39_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9207368.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3956..4597)
                     /locus_tag="IWI39_000234"
     CDS             complement(3956..4597)
                     /locus_tag="IWI39_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9207369.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4879..5187
                     /gene="rplU"
                     /locus_tag="IWI39_000235"
     CDS             4879..5187
                     /gene="rplU"
                     /locus_tag="IWI39_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9207370.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5214..5558
                     /locus_tag="IWI39_000236"
     CDS             5214..5558
                     /locus_tag="IWI39_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9207371.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5571..5864
                     /gene="rpmA"
                     /locus_tag="IWI39_000237"
     CDS             5571..5864
                     /gene="rpmA"
                     /locus_tag="IWI39_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9207372.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6271..9105
                     /locus_tag="IWI39_000238"
     CDS             6271..9105
                     /locus_tag="IWI39_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9207373.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9296..9787
                     /locus_tag="IWI39_000239"
     CDS             9296..9787
                     /locus_tag="IWI39_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9207374.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9890..10879
                     /gene="manX"
                     /locus_tag="IWI39_000240"
     CDS             9890..10879
                     /gene="manX"
                     /locus_tag="IWI39_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9207375.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10912..11715
                     /locus_tag="IWI39_000241"
     CDS             10912..11715
                     /locus_tag="IWI39_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9207376.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11736..12650
                     /locus_tag="IWI39_000242"
     CDS             11736..12650
                     /locus_tag="IWI39_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9207377.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12733..13107
                     /locus_tag="IWI39_000243"
     CDS             12733..13107
                     /locus_tag="IWI39_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9207378.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13246..13491
                     /locus_tag="IWI39_000244"
     CDS             13246..13491
                     /locus_tag="IWI39_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9207379.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13663..14727
                     /locus_tag="IWI39_000245"
     CDS             13663..14727
                     /locus_tag="IWI39_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9207380.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14973..15398
                     /locus_tag="IWI39_000246"
     CDS             14973..15398
                     /locus_tag="IWI39_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9207381.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15391..15840
                     /gene="nusB"
                     /locus_tag="IWI39_000247"
     CDS             15391..15840
                     /gene="nusB"
                     /locus_tag="IWI39_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9207382.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15937..16791
                     /locus_tag="IWI39_000248"
     CDS             15937..16791
                     /locus_tag="IWI39_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9207383.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16792..18129
                     /locus_tag="IWI39_000249"
     CDS             16792..18129
                     /locus_tag="IWI39_000249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9207384.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18134..18358
                     /locus_tag="IWI39_000250"
     CDS             18134..18358
                     /locus_tag="IWI39_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9207385.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18360..19250
                     /locus_tag="IWI39_000251"
     CDS             18360..19250
                     /locus_tag="IWI39_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9207386.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19259..20077
                     /locus_tag="IWI39_000252"
     CDS             19259..20077
                     /locus_tag="IWI39_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9207387.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20184..20633
                     /locus_tag="IWI39_000253"
     CDS             20184..20633
                     /locus_tag="IWI39_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9207388.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20651..22333
                     /gene="recN"
                     /locus_tag="IWI39_000254"
     CDS             20651..22333
                     /gene="recN"
                     /locus_tag="IWI39_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9207389.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22388..22504)
                     /locus_tag="IWI39_000255"
     CDS             complement(22388..22504)
                     /locus_tag="IWI39_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9207390.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22577..23533)
                     /locus_tag="IWI39_000256"
     CDS             complement(22577..23533)
                     /locus_tag="IWI39_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9207391.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23704..24072
                     /locus_tag="IWI39_000257"
     CDS             23704..24072
                     /locus_tag="IWI39_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9207392.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24242..24673
                     /gene="mraZ"
                     /locus_tag="IWI39_000258"
     CDS             24242..24673
                     /gene="mraZ"
                     /locus_tag="IWI39_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9207393.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24694..25653
                     /gene="rsmH"
                     /locus_tag="IWI39_000259"
     CDS             24694..25653
                     /gene="rsmH"
                     /locus_tag="IWI39_000259"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9207394.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25658..26059
                     /gene="ftsL"
                     /locus_tag="IWI39_000260"
     CDS             25658..26059
                     /gene="ftsL"
                     /locus_tag="IWI39_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9207395.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26060..28252
                     /locus_tag="IWI39_000261"
     CDS             26060..28252
                     /locus_tag="IWI39_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9207396.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28278..29240
                     /locus_tag="IWI39_000262"
     CDS             28278..29240
                     /locus_tag="IWI39_000262"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9207397.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29254..30615
                     /locus_tag="IWI39_000263"
     CDS             29254..30615
                     /locus_tag="IWI39_000263"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9207398.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30641..31729
                     /gene="murG"
                     /locus_tag="IWI39_000264"
     CDS             30641..31729
                     /gene="murG"
                     /locus_tag="IWI39_000264"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9207399.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31744..32964
                     /locus_tag="IWI39_000265"
     CDS             31744..32964
                     /locus_tag="IWI39_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9207400.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33120..34445
                     /gene="ftsA"
                     /locus_tag="IWI39_000266"
     CDS             33120..34445
                     /gene="ftsA"
                     /locus_tag="IWI39_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9207401.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34468..35709
                     /gene="ftsZ"
                     /locus_tag="IWI39_000267"
     CDS             34468..35709
                     /gene="ftsZ"
                     /locus_tag="IWI39_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9207402.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35726..36403
                     /locus_tag="IWI39_000268"
     CDS             35726..36403
                     /locus_tag="IWI39_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9207403.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36417..37016
                     /locus_tag="IWI39_000269"
     CDS             36417..37016
                     /locus_tag="IWI39_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9207404.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37031..37318
                     /locus_tag="IWI39_000270"
     CDS             37031..37318
                     /locus_tag="IWI39_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9207405.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37367..38149
                     /locus_tag="IWI39_000271"
     CDS             37367..38149
                     /locus_tag="IWI39_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9207406.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38170..38877
                     /locus_tag="IWI39_000272"
     CDS             38170..38877
                     /locus_tag="IWI39_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9207407.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39139..41937
                     /gene="ileS"
                     /locus_tag="IWI39_000273"
     CDS             39139..41937
                     /gene="ileS"
                     /locus_tag="IWI39_000273"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9207408.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41986..43512)
                     /locus_tag="IWI39_000274"
     CDS             complement(41986..43512)
                     /locus_tag="IWI39_000274"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9207409.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43527..44174)
                     /locus_tag="IWI39_000275"
     CDS             complement(43527..44174)
                     /locus_tag="IWI39_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9207410.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44358..44687)
                     /locus_tag="IWI39_000276"
     CDS             complement(44358..44687)
                     /locus_tag="IWI39_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9207411.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44864..45592
                     /gene="sfsA"
                     /locus_tag="IWI39_000277"
     CDS             44864..45592
                     /gene="sfsA"
                     /locus_tag="IWI39_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9207412.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45608..46621
                     /locus_tag="IWI39_000278"
     CDS             45608..46621
                     /locus_tag="IWI39_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9207413.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46621..47898
                     /locus_tag="IWI39_000279"
     CDS             46621..47898
                     /locus_tag="IWI39_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9207414.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47961..50663
                     /locus_tag="IWI39_000280"
     CDS             47961..50663
                     /locus_tag="IWI39_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9207415.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50815..51132
                     /locus_tag="IWI39_000281"
     CDS             50815..51132
                     /locus_tag="IWI39_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9207416.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51162..51482
                     /locus_tag="IWI39_000282"
     CDS             51162..51482
                     /locus_tag="IWI39_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9207417.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51590..53050
                     /locus_tag="IWI39_000283"
     CDS             51590..53050
                     /locus_tag="IWI39_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9207418.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53118..53339
                     /locus_tag="IWI39_000284"
     CDS             53118..53339
                     /locus_tag="IWI39_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207419.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53370..53552
                     /locus_tag="IWI39_000285"
     CDS             53370..53552
                     /locus_tag="IWI39_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9207420.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53552..53965
                     /locus_tag="IWI39_000286"
     CDS             53552..53965
                     /locus_tag="IWI39_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9207421.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54088..55269
                     /locus_tag="IWI39_000287"
     CDS             54088..55269
                     /locus_tag="IWI39_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207422.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55658..55731
                     /locus_tag="IWI39_000288"
     tRNA            55658..55731
                     /locus_tag="IWI39_000288"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55692..55694,aa:Arg,seq:cct)
     gene            complement(55800..>56306)
                     /locus_tag="IWI39_000289"
     CDS             complement(55800..>56306)
                     /locus_tag="IWI39_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9207423.1"
                     /translation="LTWEDIDFEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHT
                     IDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQF
                     TLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVF
                     DSLYKNDN"
BASE COUNT        18591 a         9505 c        12115 g        16095 t
ORIGIN      
        1 aatttttggt ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca
       61 aggaaatctg atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat
      121 tacatattat gaaaattcag gaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac
      181 aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta
      241 aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt
      301 tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca
      361 ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa
      421 tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca
      481 tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt
      541 gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact
      601 attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga
      661 agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt
      721 tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc
      781 atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt
      841 cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt
      901 gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt
      961 tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt
     1021 gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct
     1081 cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct
     1141 tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt
     1201 gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg
     1261 tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt
     1321 aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc
     1381 tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt
     1441 gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg
     1501 acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct
     1561 cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga
     1621 cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat
     1681 attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag
     1741 gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat
     1801 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
     1861 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
     1921 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
     1981 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
     2041 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
     2101 tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt
     2161 tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat
     2221 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
     2281 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
     2341 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
     2401 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
     2461 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
     2521 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
     2581 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
     2641 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
     2701 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
     2761 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
     2821 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
     2881 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
     2941 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
     3001 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
     3061 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
     3121 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
     3181 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     3241 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     3301 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     3361 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     3421 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     3481 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     3541 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     3601 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     3661 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     3721 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     3781 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     3841 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     3901 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     3961 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     4021 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     4081 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     4141 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     4201 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     4261 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     4321 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     4381 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     4441 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     4501 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     4561 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     4621 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     4681 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     4741 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     4801 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     4861 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     4921 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     4981 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     5041 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     5101 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     5161 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     5221 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     5281 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     5341 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     5401 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     5461 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     5521 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     5581 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     5641 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     5701 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     5761 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     5821 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     5881 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     5941 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     6001 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     6061 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     6121 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     6181 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     6241 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     6301 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     6361 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     6421 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     6481 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     6541 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     6601 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     6661 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     6721 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     6781 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     6841 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     6901 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     6961 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     7021 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     7081 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     7141 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     7201 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     7261 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     7321 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     7381 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     7441 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     7501 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     7561 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     7621 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     7681 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     7741 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     7801 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     7861 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     7921 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     7981 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     8041 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     8101 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     8161 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     8221 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     8281 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     8341 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     8401 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     8461 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     8521 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     8581 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     8641 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     8701 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     8761 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     8821 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     8881 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     8941 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     9001 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     9061 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     9121 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     9181 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     9241 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     9301 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     9361 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     9421 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     9481 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     9541 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     9601 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     9661 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     9721 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     9781 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     9841 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     9901 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     9961 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
    10021 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
    10081 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
    10141 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
    10201 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
    10261 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
    10321 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
    10381 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
    10441 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
    10501 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
    10561 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
    10621 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
    10681 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
    10741 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
    10801 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
    10861 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
    10921 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
    10981 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
    11041 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
    11101 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
    11161 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
    11221 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
    11281 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
    11341 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
    11401 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
    11461 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
    11521 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
    11581 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
    11641 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
    11701 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
    11761 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
    11821 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
    11881 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
    11941 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
    12001 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
    12061 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
    12121 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
    12181 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    12241 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    12301 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    12361 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    12421 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    12481 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    12541 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    12601 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    12661 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    12721 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    12781 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtaataatgg ttttgagttt
    12841 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    12901 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    12961 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    13021 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    13081 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    13141 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    13201 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    13261 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    13321 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    13381 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    13441 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    13501 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    13561 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    13621 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    13681 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    13741 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    13801 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    13861 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    13921 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    13981 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    14041 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    14101 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    14161 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    14221 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    14281 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    14341 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    14401 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    14461 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    14521 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    14581 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    14641 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    14701 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    14761 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    14821 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    14881 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    14941 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    15001 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    15061 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    15121 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    15181 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    15241 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    15301 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    15361 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    15421 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    15481 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    15541 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    15601 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    15661 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    15721 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    15781 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
    15841 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
    15901 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
    15961 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
    16021 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
    16081 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
    16141 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
    16201 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
    16261 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
    16321 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
    16381 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
    16441 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
    16501 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
    16561 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
    16621 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
    16681 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
    16741 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
    16801 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
    16861 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
    16921 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
    16981 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
    17041 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
    17101 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
    17161 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
    17221 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
    17281 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
    17341 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
    17401 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
    17461 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
    17521 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
    17581 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
    17641 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
    17701 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
    17761 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
    17821 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
    17881 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
    17941 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
    18001 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
    18061 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
    18121 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
    18181 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
    18241 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
    18301 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
    18361 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
    18421 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
    18481 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
    18541 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
    18601 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
    18661 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
    18721 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
    18781 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
    18841 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
    18901 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
    18961 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
    19021 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
    19081 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
    19141 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
    19201 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
    19261 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
    19321 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
    19381 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
    19441 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
    19501 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
    19561 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
    19621 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
    19681 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
    19741 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
    19801 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
    19861 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
    19921 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
    19981 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
    20041 ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
    20101 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
    20161 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
    20221 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
    20281 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
    20341 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
    20401 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
    20461 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
    20521 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
    20581 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
    20641 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
    20701 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
    20761 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
    20821 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
    20881 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    20941 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    21001 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    21061 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    21121 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    21181 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    21241 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    21301 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    21361 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    21421 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    21481 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    21541 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    21601 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    21661 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    21721 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    21781 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    21841 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    21901 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    21961 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    22021 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    22081 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    22141 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    22201 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    22261 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    22321 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    22381 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    22441 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    22501 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    22561 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    22621 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    22681 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    22741 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    22801 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    22861 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    22921 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    22981 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    23041 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    23101 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    23161 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    23221 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    23281 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    23341 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    23401 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    23461 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    23521 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    23581 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    23641 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    23701 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    23761 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    23821 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    23881 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    23941 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    24001 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    24061 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
    24121 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
    24181 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
    24241 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
    24301 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
    24361 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
    24421 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
    24481 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
    24541 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
    24601 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
    24661 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
    24721 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
    24781 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
    24841 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
    24901 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
    24961 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
    25021 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
    25081 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
    25141 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
    25201 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
    25261 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
    25321 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
    25381 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
    25441 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
    25501 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
    25561 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
    25621 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
    25681 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
    25741 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
    25801 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
    25861 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
    25921 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
    25981 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
    26041 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
    26101 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
    26161 tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
    26221 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
    26281 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
    26341 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
    26401 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
    26461 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
    26521 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
    26581 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
    26641 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
    26701 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
    26761 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
    26821 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
    26881 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
    26941 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
    27001 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
    27061 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
    27121 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
    27181 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
    27241 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
    27301 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
    27361 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
    27421 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
    27481 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
    27541 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
    27601 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
    27661 ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
    27721 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
    27781 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
    27841 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
    27901 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
    27961 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
    28021 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
    28081 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
    28141 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
    28201 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
    28261 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
    28321 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
    28381 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
    28441 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
    28501 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
    28561 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
    28621 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
    28681 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
    28741 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
    28801 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
    28861 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
    28921 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
    28981 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
    29041 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
    29101 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
    29161 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
    29221 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
    29281 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
    29341 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
    29401 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
    29461 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
    29521 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
    29581 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
    29641 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
    29701 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
    29761 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
    29821 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
    29881 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
    29941 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
    30001 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
    30061 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
    30121 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
    30181 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
    30241 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
    30301 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
    30361 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
    30421 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
    30481 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
    30541 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
    30601 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
    30661 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
    30721 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
    30781 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
    30841 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
    30901 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
    30961 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
    31021 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
    31081 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
    31141 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
    31201 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
    31261 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
    31321 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
    31381 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
    31441 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
    31501 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
    31561 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
    31621 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
    31681 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
    31741 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
    31801 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
    31861 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
    31921 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
    31981 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
    32041 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
    32101 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
    32161 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
    32221 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
    32281 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
    32341 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
    32401 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
    32461 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
    32521 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
    32581 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
    32641 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    32701 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    32761 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    32821 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    32881 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    32941 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    33001 aaaccagtaa tttatggtag aattagaagc agtgaatata tctattaata agtatatttt
    33061 aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca
    33121 tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg
    33181 tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag
    33241 ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag
    33301 tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag
    33361 caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg
    33421 aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc
    33481 ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa
    33541 tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag
    33601 gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca
    33661 acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag
    33721 attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg
    33781 ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata
    33841 tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg
    33901 atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt
    33961 cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac
    34021 agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg
    34081 gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa
    34141 tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat
    34201 ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg
    34261 caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa
    34321 caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac
    34381 caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg
    34441 attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac
    34501 ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg
    34561 attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta
    34621 aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt
    34681 gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc
    34741 gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt
    34801 acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt
    34861 gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt
    34921 atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta
    34981 gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta
    35041 cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac
    35101 tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg
    35161 gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt
    35221 gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg
    35281 acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta
    35341 aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc
    35401 atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa
    35461 gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact
    35521 tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt
    35581 cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac
    35641 cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt
    35701 aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga
    35761 aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt
    35821 aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg
    35881 tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga
    35941 aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga
    36001 aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt
    36061 agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg
    36121 aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa
    36181 ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt
    36241 gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc
    36301 atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc
    36361 aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc
    36421 cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg
    36481 aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa
    36541 cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg
    36601 cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg
    36661 aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta
    36721 cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg
    36781 aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt
    36841 tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac
    36901 tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg
    36961 agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg
    37021 aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt
    37081 ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc
    37141 agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct
    37201 gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt
    37261 agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag tttattgatt
    37321 gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt
    37381 ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga
    37441 acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta
    37501 tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata
    37561 tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga
    37621 agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca
    37681 tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat
    37741 catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc
    37801 tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga
    37861 aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact
    37921 agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga
    37981 agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact
    38041 attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa
    38101 gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt
    38161 ggtttactca tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg
    38221 cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag
    38281 gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt
    38341 gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg
    38401 gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa
    38461 aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat
    38521 gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg
    38581 acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg
    38641 cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc
    38701 tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac
    38761 aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca
    38821 tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac
    38881 agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact
    38941 gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg
    39001 gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg
    39061 gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat
    39121 aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc
    39181 cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat
    39241 cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat
    39301 ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa
    39361 gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg
    39421 gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa
    39481 atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag
    39541 caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca
    39601 ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc
    39661 tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg
    39721 gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc
    39781 aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca
    39841 tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg
    39901 gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa
    39961 atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg
    40021 actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact
    40081 ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga tgactatatc
    40141 gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca
    40201 gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat
    40261 ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac
    40321 gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc
    40381 gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg
    40441 ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag
    40501 cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca
    40561 ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt
    40621 gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc
    40681 gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa
    40741 gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca
    40801 gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta
    40861 gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg
    40921 agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat
    40981 atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat
    41041 atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta
    41101 gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct
    41161 gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg
    41221 tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat
    41281 ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat
    41341 tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta
    41401 ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag
    41461 gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg
    41521 atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa
    41581 gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa
    41641 caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt
    41701 tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta
    41761 tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc
    41821 caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca
    41881 aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag
    41941 ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt
    42001 tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag
    42061 tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg
    42121 tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc
    42181 ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc
    42241 ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt
    42301 ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg
    42361 aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga
    42421 tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc
    42481 agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat
    42541 agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc
    42601 gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa
    42661 gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc
    42721 tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac
    42781 ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc
    42841 gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt
    42901 gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat
    42961 ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat
    43021 ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa
    43081 acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc
    43141 aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc
    43201 atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg
    43261 ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc
    43321 acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt
    43381 tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg
    43441 gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt
    43501 ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc
    43561 ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct
    43621 gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc
    43681 aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac
    43741 tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca
    43801 tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa
    43861 gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt
    43921 tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt
    43981 ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca
    44041 agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt
    44101 ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct
    44161 cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa
    44221 caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt
    44281 tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata
    44341 tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg
    44401 tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg
    44461 actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa
    44521 aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat
    44581 gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa
    44641 ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc
    44701 aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg
    44761 taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta
    44821 caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt
    44881 ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga
    44941 cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc
    45001 aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct
    45061 gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct
    45121 ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac
    45181 ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt
    45241 ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg
    45301 tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc
    45361 cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct
    45421 ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca
    45481 aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa
    45541 gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg
    45601 taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca
    45661 agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa
    45721 agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac
    45781 ttttttcaat attactcata tggacaccat ttatattgct tctccgaata gtcttcactt
    45841 tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt
    45901 ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt
    45961 ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc
    46021 attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga
    46081 ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt
    46141 gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga
    46201 agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt
    46261 acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc
    46321 gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca
    46381 agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga
    46441 aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc
    46501 agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat
    46561 ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta
    46621 atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg
    46681 tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt
    46741 atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta
    46801 ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta
    46861 gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa
    46921 tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa
    46981 atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca
    47041 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    47101 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    47161 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    47221 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    47281 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    47341 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    47401 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    47461 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    47521 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    47581 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    47641 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    47701 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    47761 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    47821 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    47881 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    47941 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    48001 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    48061 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    48121 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    48181 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    48241 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    48301 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    48361 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    48421 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    48481 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    48541 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    48601 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    48661 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    48721 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    48781 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    48841 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    48901 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    48961 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    49021 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    49081 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    49141 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    49201 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    49261 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    49321 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    49381 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    49441 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    49501 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    49561 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    49621 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    49681 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    49741 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    49801 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    49861 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    49921 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    49981 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    50041 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    50101 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    50161 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    50221 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    50281 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    50341 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    50401 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    50461 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    50521 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    50581 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    50641 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    50701 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    50761 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    50821 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    50881 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    50941 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    51001 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    51061 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    51121 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    51181 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    51241 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    51301 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    51361 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    51421 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    51481 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    51541 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    51601 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    51661 tttagttgct ttgcgtgatg cttttatcgg catactgccc gcaacaatgg cgggatcagt
    51721 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    51781 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    51841 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    51901 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    51961 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    52021 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    52081 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    52141 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    52201 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    52261 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    52321 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct
    52381 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    52441 gttctttggt attcatggat caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    52501 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    52561 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    52621 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    52681 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    52741 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    52801 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    52861 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    52921 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    52981 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    53041 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    53101 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    53161 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    53221 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    53281 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    53341 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    53401 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    53461 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    53521 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    53581 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    53641 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    53701 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    53761 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    53821 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    53881 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    53941 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    54001 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    54061 agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    54121 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    54181 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    54241 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    54301 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    54361 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    54421 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    54481 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    54541 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    54601 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    54661 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    54721 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    54781 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    54841 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    54901 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    54961 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    55021 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    55081 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    55141 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    55201 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    55261 tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt
    55321 tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt
    55381 tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt
    55441 cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat
    55501 tcattggaaa ggacctccct acattatttc attctacctc tagtttatca tctgttaagg
    55561 actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag
    55621 taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag
    55681 agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa
    55741 cccctgtatc ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac
    55801 tagttatcat ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct
    55861 tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct
    55921 aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta
    55981 tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc
    56041 aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa
    56101 ttatgagggt ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt
    56161 tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt
    56221 gtttttcccc attcatttaa tttataattc catgttttat taatagtgat gatttgatct
    56281 tcgaaatcga tatcttccca tgttaa
//
LOCUS       DACDOA010000006        55502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDOA010000006 DACDOA010000000
VERSION     DACDOA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.006"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            558..3197
                     /locus_tag="IWI39_000290"
     CDS             558..3197
                     /locus_tag="IWI39_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9207424.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="IWI39_000291"
     CDS             complement(3365..4063)
                     /locus_tag="IWI39_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9207425.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="IWI39_000292"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="IWI39_000292"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9207426.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="IWI39_000293"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="IWI39_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9207427.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="IWI39_000294"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="IWI39_000294"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9207428.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="IWI39_000295"
     CDS             9034..10140
                     /locus_tag="IWI39_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9207429.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="IWI39_000296"
     CDS             10157..11185
                     /locus_tag="IWI39_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9207430.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="IWI39_000297"
     CDS             11191..11670
                     /locus_tag="IWI39_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9207431.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="IWI39_000298"
     CDS             11686..12981
                     /locus_tag="IWI39_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9207432.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="IWI39_000299"
     CDS             12992..13816
                     /locus_tag="IWI39_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9207433.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="IWI39_000300"
     CDS             13981..14880
                     /locus_tag="IWI39_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9207434.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="IWI39_000301"
     CDS             complement(15006..15875)
                     /locus_tag="IWI39_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9207435.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="IWI39_000302"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="IWI39_000302"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9207436.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="IWI39_000303"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="IWI39_000303"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9207437.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="IWI39_000304"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="IWI39_000304"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9207438.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="IWI39_000305"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="IWI39_000305"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9207439.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="IWI39_000306"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="IWI39_000306"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9207440.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="IWI39_000307"
     CDS             21273..22031
                     /locus_tag="IWI39_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9207441.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="IWI39_000308"
     CDS             22131..22775
                     /locus_tag="IWI39_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9207442.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="IWI39_000309"
     CDS             22782..23789
                     /locus_tag="IWI39_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9207443.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="IWI39_000310"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="IWI39_000310"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9207444.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="IWI39_000311"
     CDS             24667..25470
                     /locus_tag="IWI39_000311"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9207445.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="IWI39_000312"
     CDS             complement(25719..26783)
                     /locus_tag="IWI39_000312"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9207446.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="IWI39_000313"
     CDS             complement(26780..27865)
                     /locus_tag="IWI39_000313"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9207447.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="IWI39_000314"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="IWI39_000314"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9207448.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="IWI39_000315"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="IWI39_000315"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9207449.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30114..30767
                     /locus_tag="IWI39_000316"
     CDS             30114..30767
                     /locus_tag="IWI39_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9207450.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            30852..31757
                     /locus_tag="IWI39_000317"
     CDS             30852..31757
                     /locus_tag="IWI39_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9207451.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="IWI39_000318"
     CDS             complement(31759..32391)
                     /locus_tag="IWI39_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9207452.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="IWI39_000319"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="IWI39_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9207453.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="IWI39_000320"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="IWI39_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9207454.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="IWI39_000321"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="IWI39_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9207455.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="IWI39_000322"
     CDS             34171..35508
                     /locus_tag="IWI39_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9207456.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="IWI39_000323"
     CDS             35556..37685
                     /locus_tag="IWI39_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9207457.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="IWI39_000324"
     CDS             37660..38349
                     /locus_tag="IWI39_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207458.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="IWI39_000325"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="IWI39_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="IWI39_000326"
     CDS             complement(39506..39700)
                     /locus_tag="IWI39_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207459.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="IWI39_000327"
     CDS             complement(39750..40190)
                     /locus_tag="IWI39_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9207460.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="IWI39_000328"
     CDS             complement(40194..41039)
                     /locus_tag="IWI39_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9207461.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="IWI39_000329"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="IWI39_000329"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9207462.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="IWI39_000330"
     CDS             complement(42663..43610)
                     /locus_tag="IWI39_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9207463.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="IWI39_000331"
     CDS             complement(43833..44183)
                     /locus_tag="IWI39_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9207464.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="IWI39_000332"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="IWI39_000332"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9207465.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="IWI39_000333"
     CDS             45606..45926
                     /locus_tag="IWI39_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9207466.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="IWI39_000334"
     CDS             45948..46412
                     /locus_tag="IWI39_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9207467.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /locus_tag="IWI39_000335"
     CDS             46617..47222
                     /locus_tag="IWI39_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9207468.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="IWI39_000336"
     CDS             complement(47261..48550)
                     /locus_tag="IWI39_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9207469.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="IWI39_000337"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="IWI39_000337"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9207470.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="IWI39_000338"
     CDS             49542..50051
                     /locus_tag="IWI39_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9207471.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="IWI39_000339"
     CDS             50151..50801
                     /locus_tag="IWI39_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207472.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="IWI39_000340"
     CDS             51252..52190
                     /locus_tag="IWI39_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9207473.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="IWI39_000341"
     CDS             52203..53255
                     /locus_tag="IWI39_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9207474.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="IWI39_000342"
     CDS             53521..54156
                     /locus_tag="IWI39_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9207475.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="IWI39_000343"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="IWI39_000343"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9207476.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DACDOA010000007        54959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDOA010000007 DACDOA010000000
VERSION     DACDOA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.007"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            993..1319
                     /locus_tag="IWI39_000344"
     CDS             993..1319
                     /locus_tag="IWI39_000344"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9207477.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1327..2235
                     /locus_tag="IWI39_000345"
     CDS             1327..2235
                     /locus_tag="IWI39_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9207478.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2864..3115
                     /locus_tag="IWI39_000346"
     CDS             2864..3115
                     /locus_tag="IWI39_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP9207479.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3657..3815
                     /locus_tag="IWI39_000347"
     CDS             3657..3815
                     /locus_tag="IWI39_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9207480.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            3887..5113
                     /locus_tag="IWI39_000348"
     CDS             3887..5113
                     /locus_tag="IWI39_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9207481.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5202..5594)
                     /gene="rpsI"
                     /locus_tag="IWI39_000349"
     CDS             complement(5202..5594)
                     /gene="rpsI"
                     /locus_tag="IWI39_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9207482.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5610..6053)
                     /gene="rplM"
                     /locus_tag="IWI39_000350"
     CDS             complement(5610..6053)
                     /gene="rplM"
                     /locus_tag="IWI39_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9207483.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6572..7039)
                     /locus_tag="IWI39_000351"
     CDS             complement(6572..7039)
                     /locus_tag="IWI39_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9207484.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7159..8697)
                     /locus_tag="IWI39_000352"
     CDS             complement(7159..8697)
                     /locus_tag="IWI39_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9207485.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8777..9463
                     /locus_tag="IWI39_000353"
     CDS             8777..9463
                     /locus_tag="IWI39_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9207486.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGTSVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9664..10191)
                     /gene="msrA"
                     /locus_tag="IWI39_000354"
     CDS             complement(9664..10191)
                     /gene="msrA"
                     /locus_tag="IWI39_000354"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9207487.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10275..10952)
                     /gene="pgmB"
                     /locus_tag="IWI39_000355"
     CDS             complement(10275..10952)
                     /gene="pgmB"
                     /locus_tag="IWI39_000355"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9207488.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(10949..12077)
                     /locus_tag="IWI39_000356"
                     /pseudo
     CDS             complement(10949..12077)
                     /locus_tag="IWI39_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12077..14260)
                     /locus_tag="IWI39_000357"
     CDS             complement(12077..14260)
                     /locus_tag="IWI39_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9207489.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14257..15282)
                     /locus_tag="IWI39_000358"
     CDS             complement(14257..15282)
                     /locus_tag="IWI39_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9207490.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15327..16154)
                     /locus_tag="IWI39_000359"
     CDS             complement(15327..16154)
                     /locus_tag="IWI39_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9207491.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16151..17218)
                     /locus_tag="IWI39_000360"
     CDS             complement(16151..17218)
                     /locus_tag="IWI39_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9207492.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17244..18080)
                     /locus_tag="IWI39_000361"
     CDS             complement(17244..18080)
                     /locus_tag="IWI39_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207493.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18077..18946)
                     /locus_tag="IWI39_000362"
     CDS             complement(18077..18946)
                     /locus_tag="IWI39_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207494.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19016..20299)
                     /locus_tag="IWI39_000363"
     CDS             complement(19016..20299)
                     /locus_tag="IWI39_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9207495.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20313..21998)
                     /locus_tag="IWI39_000364"
     CDS             complement(20313..21998)
                     /locus_tag="IWI39_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9207496.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22273..23298
                     /locus_tag="IWI39_000365"
     CDS             22273..23298
                     /locus_tag="IWI39_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207497.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(23893..27546)
                     /gene="rpoC"
                     /locus_tag="IWI39_000366"
     CDS             complement(23893..27546)
                     /gene="rpoC"
                     /locus_tag="IWI39_000366"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9207498.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27625..31242)
                     /gene="rpoB"
                     /locus_tag="IWI39_000367"
     CDS             complement(27625..31242)
                     /gene="rpoB"
                     /locus_tag="IWI39_000367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9207499.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            31970..32938
                     /locus_tag="IWI39_000368"
     CDS             31970..32938
                     /locus_tag="IWI39_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207500.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            32956..33861
                     /locus_tag="IWI39_000369"
     CDS             32956..33861
                     /locus_tag="IWI39_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207501.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            33858..34664
                     /locus_tag="IWI39_000370"
     CDS             33858..34664
                     /locus_tag="IWI39_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9207502.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            34821..35789
                     /locus_tag="IWI39_000371"
     CDS             34821..35789
                     /locus_tag="IWI39_000371"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9207503.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36106..36630)
                     /locus_tag="IWI39_000372"
     CDS             complement(36106..36630)
                     /locus_tag="IWI39_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9207504.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            36917..38869
                     /gene="scm"
                     /locus_tag="IWI39_000373"
     CDS             36917..38869
                     /gene="scm"
                     /locus_tag="IWI39_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9207505.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTS
                     TTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTST
                     TSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTS
                     SDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNN
                     WITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(39024..40010)
                     /locus_tag="IWI39_000374"
     CDS             complement(39024..40010)
                     /locus_tag="IWI39_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9207506.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(40033..40599)
                     /locus_tag="IWI39_000375"
     CDS             complement(40033..40599)
                     /locus_tag="IWI39_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9207507.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(40613..41029)
                     /locus_tag="IWI39_000376"
     CDS             complement(40613..41029)
                     /locus_tag="IWI39_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9207508.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(41486..43420)
                     /locus_tag="IWI39_000377"
     CDS             complement(41486..43420)
                     /locus_tag="IWI39_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9207509.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            43722..44657
                     /locus_tag="IWI39_000378"
     CDS             43722..44657
                     /locus_tag="IWI39_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9207510.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            44747..45850
                     /locus_tag="IWI39_000379"
     CDS             44747..45850
                     /locus_tag="IWI39_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9207511.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            45863..46816
                     /gene="menA"
                     /locus_tag="IWI39_000380"
     CDS             45863..46816
                     /gene="menA"
                     /locus_tag="IWI39_000380"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9207512.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(47751..49097)
                     /gene="gor"
                     /locus_tag="IWI39_000381"
     CDS             complement(47751..49097)
                     /gene="gor"
                     /locus_tag="IWI39_000381"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9207513.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(49231..50472)
                     /locus_tag="IWI39_000382"
     CDS             complement(49231..50472)
                     /locus_tag="IWI39_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9207514.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(50490..51413)
                     /locus_tag="IWI39_000383"
     CDS             complement(50490..51413)
                     /locus_tag="IWI39_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9207515.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(51634..54126)
                     /locus_tag="IWI39_000384"
     CDS             complement(51634..54126)
                     /locus_tag="IWI39_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9207516.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(54133..54600)
                     /locus_tag="IWI39_000385"
     CDS             complement(54133..54600)
                     /locus_tag="IWI39_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9207517.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(54736..54809)
                     /locus_tag="IWI39_000386"
     tRNA            complement(54736..54809)
                     /locus_tag="IWI39_000386"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54773..54775),aa:Arg,seq:acg)
     gene            complement(54867..54940)
                     /locus_tag="IWI39_000387"
     tRNA            complement(54867..54940)
                     /locus_tag="IWI39_000387"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54904..54906),aa:Pro,seq:tgg)
BASE COUNT        15981 a        11594 c        10099 g        17285 t
ORIGIN      
        1 aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga tatcatcaca
       61 ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag atttcactgt
      121 gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta atctaagcga
      181 tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg gaaaaacttt
      241 tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc ctaccacacg
      301 ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct ccaaaaacaa
      361 aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc gtccgcctcg
      421 gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat aagataaatc
      481 atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg taaaaactta
      541 tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt acccacctgc
      601 acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc attataatga
      661 ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa ggcaccaatg
      721 aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac cctttccttg
      781 caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta tagcggcaac
      841 cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc gcaaacccgt
      901 agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc agtccctacc
      961 aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt aaaaattgtt
     1021 gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca gatgcgaatg
     1081 gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc aatgaattag
     1141 ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc ggtaaatttt
     1201 ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct catccgaagt
     1261 acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg gatttgtgat
     1321 ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat ccgccagctt
     1381 cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga aaagaatcgt
     1441 gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag tgatgtgttc
     1501 ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga atcatttgat
     1561 gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca agcggcagtt
     1621 gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa ccatattaac
     1681 gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc agaacccttc
     1741 tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc aataacagcg
     1801 attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc gaaacacttt
     1861 atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac aaaatcaaca
     1921 cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc caatgctctt
     1981 ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa agcgaatcgt
     2041 ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat ctggaatgtc
     2101 ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc tgcgaaagaa
     2161 gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg atataccatt
     2221 atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg ttgtcttcca
     2281 tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg tatcttcaaa
     2341 tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct tagcatttaa
     2401 gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc tcctagtata
     2461 taggcagact gccaattata actgattcaa tccacattat gataaaatcc accttttcgt
     2521 ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca ttcagtgctt
     2581 tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt acaaaatcag
     2641 aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg caacagattg
     2701 gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg ctgtttccca
     2761 gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag caattgacta
     2821 cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat caaatcaaaa
     2881 atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg actgtatcat
     2941 aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat tggacagttt
     3001 tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct cgactcttgt
     3061 ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa aatagaaaag
     3121 agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct ttgtggatcc
     3181 attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg tatttgataa
     3241 tttatcttaa caaccagata attttattca gcaccgtatt atctatattg aattctcgga
     3301 atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg ttattaatag
     3361 tgattaggat gatattccta tattgatctt tgataattag atataatttt tgaataacgt
     3421 tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga caactactta
     3481 gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa taatgatact
     3541 tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaaagcgg
     3601 ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa gcctacttga
     3661 ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat aagtggattc
     3721 gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac gtagaggata
     3781 tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc acatatacta
     3841 taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc ctacaaagct
     3901 ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag aaatcactgg
     3961 aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta ttaaggccga
     4021 aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa actcactaga
     4081 attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc ctagatatga
     4141 actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta gtaatactaa
     4201 ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga attatctgaa
     4261 ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat acgcaaatat
     4321 aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac tatgttatat
     4381 cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga aaatggcttt
     4441 agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc tgactgattg
     4501 gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg attacacact
     4561 tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc tgcaatggaa
     4621 atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata aatatcaaag
     4681 aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag ttgtgatgac
     4741 attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa aaattataca
     4801 aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa attgtcctgt
     4861 tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc ctgaattggc
     4921 tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg aaggtggagc
     4981 aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta caaaaatata
     5041 cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa aaggattgaa
     5101 taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga cactttccaa
     5161 ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt tgagaattgt
     5221 gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
     5281 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
     5341 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     5401 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     5461 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     5521 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     5581 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     5641 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     5701 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     5761 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     5821 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
     5881 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     5941 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     6001 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     6061 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     6121 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     6181 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
     6241 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
     6301 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
     6361 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
     6421 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
     6481 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     6541 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
     6601 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
     6661 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
     6721 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
     6781 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
     6841 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
     6901 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
     6961 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
     7021 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
     7081 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
     7141 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
     7201 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
     7261 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
     7321 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
     7381 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
     7441 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
     7501 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
     7561 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
     7621 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
     7681 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
     7741 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
     7801 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
     7861 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
     7921 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
     7981 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
     8041 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
     8101 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
     8161 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
     8221 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
     8281 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
     8341 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
     8401 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
     8461 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
     8521 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
     8581 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
     8641 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
     8701 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
     8761 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
     8821 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
     8881 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
     8941 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
     9001 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
     9061 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
     9121 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
     9181 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
     9241 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
     9301 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaacatccgt
     9361 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
     9421 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
     9481 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
     9541 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
     9601 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
     9661 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
     9721 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
     9781 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
     9841 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
     9901 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
     9961 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
    10021 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
    10081 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
    10141 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
    10201 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
    10261 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
    10321 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
    10381 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
    10441 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
    10501 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
    10561 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
    10621 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
    10681 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
    10741 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
    10801 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
    10861 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
    10921 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
    10981 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
    11041 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
    11101 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
    11161 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
    11221 cttcgcctac gattacgagg tctgcttgct gtacccgtat cgaactccag taaatccaat
    11281 acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt
    11341 ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa
    11401 tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg
    11461 aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg
    11521 attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct
    11581 agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga
    11641 ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg
    11701 tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac
    11761 ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca
    11821 aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta
    11881 gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct
    11941 tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa
    12001 cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc
    12061 gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt
    12121 attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt
    12181 gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt
    12241 tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac
    12301 ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt
    12361 catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat
    12421 acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg
    12481 atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag
    12541 aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga
    12601 gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt
    12661 tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct
    12721 gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa
    12781 aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt
    12841 tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta
    12901 tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa
    12961 tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt
    13021 gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt
    13081 gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt
    13141 gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg
    13201 cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat
    13261 ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt
    13321 cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct
    13381 gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg
    13441 ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg
    13501 tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa
    13561 gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt
    13621 ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt
    13681 tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat
    13741 cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct
    13801 gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca
    13861 ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca
    13921 ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat
    13981 ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga
    14041 tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta
    14101 aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct
    14161 cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat
    14221 tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt
    14281 ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt gccagccatc
    14341 tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc
    14401 atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc
    14461 gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag
    14521 tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc
    14581 taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc
    14641 gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt
    14701 tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat
    14761 caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga
    14821 ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt
    14881 gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt
    14941 tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc
    15001 gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt
    15061 ggctgtacag acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt
    15121 atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc
    15181 aaccacagcg gccaattcta agacatctga tcttttttga taataagcag catgtacttt
    15241 ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct ctctcctttg
    15301 cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct
    15361 gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct
    15421 cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag tagcgtttga
    15481 aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa
    15541 tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca
    15601 atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg
    15661 taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc
    15721 aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg
    15781 actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg
    15841 aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc
    15901 aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc
    15961 cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt
    16021 ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca
    16081 cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc
    16141 gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg
    16201 ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat
    16261 caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga
    16321 ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga
    16381 cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt
    16441 tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta
    16501 atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt
    16561 cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt
    16621 tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg
    16681 cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag
    16741 catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt
    16801 aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa
    16861 caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt
    16921 gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt
    16981 gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc
    17041 attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg
    17101 ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac
    17161 ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct
    17221 tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta
    17281 ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg
    17341 gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt
    17401 aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa
    17461 accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc
    17521 cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc
    17581 ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc
    17641 atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat
    17701 aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt
    17761 ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact
    17821 actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat
    17881 ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa
    17941 atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc
    18001 aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc
    18061 ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg
    18121 caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa
    18181 aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat
    18241 ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt
    18301 tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag
    18361 ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat
    18421 acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt
    18481 cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga
    18541 ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa
    18601 cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca
    18661 aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa
    18721 ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta
    18781 atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa
    18841 tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa
    18901 gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct
    18961 gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt
    19021 tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt
    19081 ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct
    19141 tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca
    19201 gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt
    19261 tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt
    19321 gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag
    19381 aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa
    19441 aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga
    19501 ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc
    19561 ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg
    19621 tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt
    19681 gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt
    19741 tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt
    19801 ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc
    19861 taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt
    19921 cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca
    19981 gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt
    20041 taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt
    20101 gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac
    20161 ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt
    20221 tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag
    20281 taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat
    20341 tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac
    20401 aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg
    20461 ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag
    20521 tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc
    20581 atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca
    20641 atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg
    20701 cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt
    20761 aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct
    20821 gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca
    20881 tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc cactaattca
    20941 aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag
    21001 gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct
    21061 ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac
    21121 tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt
    21181 ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg
    21241 aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc
    21301 cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa
    21361 agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct
    21421 tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt
    21481 aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata
    21541 aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca
    21601 gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata
    21661 tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca
    21721 tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac
    21781 ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc
    21841 ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc
    21901 ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct
    21961 gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg
    22021 gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt
    22081 aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag
    22141 cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca
    22201 tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag
    22261 gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa
    22321 tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt
    22381 gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca
    22441 ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc
    22501 aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc
    22561 gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag
    22621 atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt
    22681 ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac
    22741 tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa
    22801 acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat
    22861 gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc
    22921 cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat
    22981 gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca
    23041 ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc
    23101 gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac
    23161 attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac
    23221 caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc
    23281 caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt
    23341 tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat
    23401 ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact
    23461 attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt
    23521 tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga tacttatctc
    23581 taaaacataa atagaataac agacatgtct actggttttg ttttattcga ggaattattc
    23641 aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca
    23701 ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc
    23761 gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt
    23821 agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt cacaacctct
    23881 tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca tttgcgcttc
    23941 aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg gttccatgtc
    24001 acgataacgt tgcataccag taccagctgg gatgatctta cctataataa cattttcttt
    24061 caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt
    24121 ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt ttgtgatacc
    24181 tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat tgtagttttg
    24241 atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat cacctggatc
    24301 cattacacga actttacgca gcatttgacg aaccattact tcgatatgct tgtcgccgat
    24361 ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt tttcaacaga
    24421 taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg tcaatggtgc
    24481 accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag gaacagtata
    24541 cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg caggatcttc
    24601 tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt tcggattacg
    24661 tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc cggcaacccc
    24721 acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg attgagcggc
    24781 gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta
    24841 acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga ttgtcactgt
    24901 ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc
    24961 aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac gtcccactaa
    25021 acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga tttcaagacc
    25081 tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt caaccagacg
    25141 acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc ctttacgagc
    25201 cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt tcgaaatgat
    25261 tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc cggcaagctg
    25321 tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga ttggattttt
    25381 cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat tccaaacagc
    25441 aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt gttttgtaat
    25501 agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca gtacactgat
    25561 gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta agtctttcat
    25621 acggtcaagc atcttagaag tttcttttac tttgaatcgt ttgaagactt ctgcgatgat
    25681 gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga tatgtgcagg
    25741 aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca agttgaagtc
    25801 tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga tgatcttacc
    25861 aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt cacctaatga
    25921 gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac
    25981 ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt cttccattgt
    26041 caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt taccatcttt
    26101 agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt gtgcttcttc
    26161 attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat aagcttcaca
    26221 tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga atgcttggat
    26281 accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct tgattacgtc
    26341 ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg catttttgat
    26401 gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga ataattcaat
    26461 ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa caacgataac
    26521 ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac gaccttgttt
    26581 ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag taactggacg
    26641 tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac gtttttcatt
    26701 ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt tattacggtt
    26761 gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac cttctaattg
    26821 aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca tccagcttgg
    26881 ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg cacgtgtacg
    26941 tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg catctagatc
    27001 aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga attcttgtcc
    27061 atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt tctctaaatt
    27121 cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt ccaaagcgcg
    27181 tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt accagatatg
    27241 tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt tcgaacgagt
    27301 aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt tgtattttcc
    27361 gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg
    27421 ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac cgtaagacca
    27481 gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt tatttacatc
    27541 gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc tgatcttcga
    27601 ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt caaagttttg
    27661 gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta attctttggc
    27721 agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc
    27781 gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca tgtccagacc
    27841 tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg gttttggaat
    27901 tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt catctgattt
    27961 gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg cccatacttc
    28021 catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt gttgtgtaac
    28081 caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt gagcaagttt
    28141 gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg tacgtccgtc
    28201 gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt cccatacgtc
    28261 ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact gacgtgcagc
    28321 catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca cccctaaagg
    28381 attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt cttccggcat
    28441 aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc cttcatggat
    28501 tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg acaattcatc
    28561 tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc catgtggtac
    28621 acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat gcaatagacg
    28681 ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc
    28741 atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt ttaatgcgtc
    28801 ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc
    28861 ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta ccaaacgacg
    28921 gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac caactaaaac
    28981 gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca gagtatcccc
    29041 tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg ttccggagtt
    29101 tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg
    29161 gatttcagaa gcatcaacgt attcaacgac accatcatgt ttacataaca aagcagctcc
    29221 tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt gaggattgat
    29281 caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg agtcatcgtt
    29341 ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg atacgtccat
    29401 gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag ccataacaac
    29461 gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta cgatgtaatg
    29521 gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg tttcacggtc
    29581 tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat aactggacaa
    29641 actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca tacggccata
    29701 gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca atccgccagg
    29761 tcctaaggcg aaaagacgac gtttgtgtgt caattcgcca agagggtttg tttgatccat
    29821 gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa ctggacggat
    29881 attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc tttcacgaac
    29941 cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt caccgactga
    30001 acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac cttccatcaa
    30061 gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt ttacagctgc
    30121 ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg gtgaaaatac
    30181 tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg gatagtaagt
    30241 cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt ggtgagtcag
    30301 aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg tttcagctaa
    30361 tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt aacgaccaac
    30421 gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct
    30481 gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca aaccttcttc
    30541 tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc gtaatgtttc
    30601 gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg cacgaactaa
    30661 tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt cttttgcatc
    30721 agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga atccttcttt
    30781 tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta actgagaaac
    30841 gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta atgggaaatc
    30901 accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca aacgcaatgt
    30961 tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta ctgtatactt
    31021 tggttctttc aattcataat ctacaaattc caaagataga ttgccattga agtcatcgat
    31081 tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt ggtaagagtc
    31141 tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg cgaaacttct
    31201 acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca cccctctaaa
    31261 atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat attattttta
    31321 ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat agaacattct
    31381 cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc cgaaacggac
    31441 aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata tactacactt
    31501 attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa cattttctta
    31561 atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga cctttttatc
    31621 tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc aacatcaacc
    31681 aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag gtttgtattc
    31741 atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa agaactttgt
    31801 attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa aatacaaagt
    31861 aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc catttggaca
    31921 ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa
    31981 tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg cacaaaaaga
    32041 caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac ttgtacaaca
    32101 taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag aagggtataa
    32161 agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag acaacctgaa
    32221 aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa tcgccactcc
    32281 agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca cagcggtcac
    32341 agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca atgtgactgg
    32401 cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga
    32461 cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga tccaagctga
    32521 tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc
    32581 cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg gaatctatat
    32641 tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac
    32701 aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt
    32761 aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa aaatcttaga
    32821 cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa
    32881 taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag ctgaataaaa
    32941 cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc atcgcaaggg
    33001 gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt ggacattgcc
    33061 gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt aagtattaca
    33121 aatggctatc atccattgac agcttgtctt ctaggctttt tagggagtgc ggcagcaggg
    33181 cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc agggatcatc
    33241 accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta
    33301 ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa agacaacgct
    33361 gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt tttgttctta
    33421 cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat gagcgaagca
    33481 aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa tggatgcatt
    33541 gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt
    33601 ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt acgtaaccag
    33661 ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta
    33721 gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc tgctttcgtg
    33781 ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag
    33841 aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat catacctttg
    33901 aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc ttagatcccg
    33961 gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg ctgaacagca
    34021 tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa aatatcacga
    34081 aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat cctaaactag
    34141 ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa
    34201 aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa gaccagctgg
    34261 ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag
    34321 gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa ctgatcttat
    34381 tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg gaattgacag
    34441 aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac atggaagacg
    34501 cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc
    34561 agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc aaacaaaaca
    34621 gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa agaggatggg
    34681 acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt tttgctttat
    34741 gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg atagaatgga
    34801 tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta ttgaaaaaga
    34861 caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc cggacaagta
    34921 tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac gggcctacag
    34981 ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa gagctacggt
    35041 cgtttattcc tgatttgaaa gtacaaacga tgatgacttc tgaatctacg atgaaagatg
    35101 ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct gatacacaag
    35161 aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga ggaatctaca
    35221 tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat acgatcatcg
    35281 cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat
    35341 gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct gaagcagtca
    35401 gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt aatttttctg
    35461 ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc tttcccgacg
    35521 gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt
    35581 atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc gtattgggaa
    35641 aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg
    35701 atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct tctggcgaaa
    35761 tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga cacctgatct
    35821 catacagtgt ccttttttct gaattctctt caaaagactc tccttattct atcagctgac
    35881 agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat ggtacacttt
    35941 ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc aaaagagata
    36001 gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa aaacaaaaaa
    36061 gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat ctcttaatcc
    36121 caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat caataataga
    36181 aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta cttcatctag
    36241 ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta
    36301 tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca gactatgctt
    36361 ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt cttgactatg
    36421 ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc gagcggtatg
    36481 ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg gaaaacgatt
    36541 ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg cccgatccac
    36601 actctcgcat agcatatcta aataatccat ttattttcct cctttttatt tatcctactt
    36661 actttttcct aattctagga tacctactta tatgttacta cacaccctta ttttttacac
    36721 tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt gtcgaactat
    36781 caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat aaacaatgat
    36841 ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa tgtttgaaag
    36901 gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg ctttgttgtt
    36961 caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta aagaccctgg
    37021 gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta gtcaattcca
    37081 cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata tagcaacaca
    37141 tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat tgaaccaaga
    37201 aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac ctacaggtag
    37261 cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc aagatggtca
    37321 tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt ataaagatcg
    37381 tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg tagcagcaaa
    37441 tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc cagacatgaa
    37501 caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg taaatatcga
    37561 tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg atcaagtagt
    37621 tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa tcaaatacaa
    37681 tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt ggaattttgg
    37741 aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa
    37801 tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa atgtgatttt
    37861 agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca atggtacaga
    37921 tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct cagattcatc
    37981 aacttcaagc gacagtacag atactacctc taccacaagt gatagcacgg atacatcagc
    38041 ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggaca catcagcttc
    38101 aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct ctaccacaag
    38161 tgacagcacg gacacgtcag cttcaagcga cagtacagat actacctcta ccacaagtga
    38221 tagcacggat acatcagctt caagcgacag tacagatact acctctacca caagtgatag
    38281 cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagcac
    38341 ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga
    38401 cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gcacggacac
    38461 gtcagcttca agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc
    38521 taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc
    38581 taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac
    38641 cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac
    38701 gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact
    38761 tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt
    38821 aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca
    38881 agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa
    38941 atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat
    39001 ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa
    39061 aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc
    39121 ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg
    39181 attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt
    39241 agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga
    39301 cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata
    39361 tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg
    39421 aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca
    39481 gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga
    39541 tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg
    39601 agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag
    39661 tctcctgcat agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt
    39721 cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg
    39781 atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac
    39841 aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgattgct
    39901 tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc
    39961 aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc
    40021 cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc
    40081 ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat
    40141 cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac
    40201 tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc
    40261 gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa
    40321 cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc
    40381 gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt cttgtttttt
    40441 catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc
    40501 aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac
    40561 aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtaaag gtctaataaa
    40621 tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca
    40681 aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat
    40741 cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc
    40801 cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt
    40861 tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct
    40921 gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa
    40981 catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc
    41041 tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc
    41101 ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg
    41161 ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag
    41221 tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa
    41281 tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa
    41341 cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt
    41401 aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg
    41461 cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt
    41521 accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg
    41581 cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt
    41641 aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc
    41701 atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt
    41761 tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc
    41821 agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa
    41881 aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg
    41941 ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc
    42001 aagggaacac actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga
    42061 acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga
    42121 aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca
    42181 cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac atataataac
    42241 cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa
    42301 atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa
    42361 ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca
    42421 cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg
    42481 gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt
    42541 cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta
    42601 atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag
    42661 tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg
    42721 cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat
    42781 ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt
    42841 ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca
    42901 tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt
    42961 catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta
    43021 cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt
    43081 cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg
    43141 tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat
    43201 tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc
    43261 cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag
    43321 taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg
    43381 catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag
    43441 actatctata taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc
    43501 atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt
    43561 aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt
    43621 tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa
    43681 agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg
    43741 taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg
    43801 acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa
    43861 gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata
    43921 aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag
    43981 acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa
    44041 aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca
    44101 ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa
    44161 ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg
    44221 ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca
    44281 cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag
    44341 ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa
    44401 aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa
    44461 cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac
    44521 ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg
    44581 cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata
    44641 tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc
    44701 cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact
    44761 actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc
    44821 agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc
    44881 tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga
    44941 atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa
    45001 tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt
    45061 ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg
    45121 cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc
    45181 cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat
    45241 cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt
    45301 aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg
    45361 agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag
    45421 aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt
    45481 tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta
    45541 tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt
    45601 cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt
    45661 gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt
    45721 gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc
    45781 aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga
    45841 actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg
    45901 aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt
    45961 tcatgagata cattggctaa acactttttt attttttatt ggaatgctag tttttgattt
    46021 agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa
    46081 gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat
    46141 tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg
    46201 gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc
    46261 gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg
    46321 aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt
    46381 caacttttca tctggacaat ttgctttggt aggaaatctt tgggctatag cagcaatcgt
    46441 attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg
    46501 ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc
    46561 tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg
    46621 gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt
    46681 gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc
    46741 catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat
    46801 tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata
    46861 agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag
    46921 atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta
    46981 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
    47041 agaaactggc ttttgaacac cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt
    47101 attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    47161 gagaaactgg cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag
    47221 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    47281 gaaggacagc tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt
    47341 tttcacagtc ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc
    47401 ttggtttggt aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt
    47461 atacaaagtc acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca
    47521 tcgataccaa tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca
    47581 gttacgaaga taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa
    47641 gcaatcttac ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg
    47701 gttttacatg gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc
    47761 gttacgaatt cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta
    47821 gttgcaccca tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca
    47881 atagcatgta agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat
    47941 ttttggcgat aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc
    48001 aattcatttt cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt
    48061 ggtggatgcg taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt
    48121 ccattaaata aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct
    48181 aatttaccga tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta
    48241 tctactttga taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt
    48301 tcggtattcg gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca
    48361 ttttcaaata gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt
    48421 gcgtaagttt ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta
    48481 tcaaacgttc taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca
    48541 cgaaatacac ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta
    48601 ggtaactctg ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg
    48661 gtcattttac ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca
    48721 ccgtttactt cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca
    48781 tgattgctat ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt
    48841 ctttctacta gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag
    48901 gcatctcgtt caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca
    48961 caaccgacgt tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg
    49021 ccatgcatcc ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg
    49081 taatcatata tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta
    49141 acgatccttt ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat
    49201 gcttaaaaca gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg
    49261 tttcaccggc tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat
    49321 ggattcgcct tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg
    49381 accataaaat tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat
    49441 ctgtgttgcc tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg
    49501 acgttcaccg aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa
    49561 ttctcgttgt gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc
    49621 aggatcttcc atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata
    49681 atggatcgat ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc
    49741 tggaatatgt tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc
    49801 gcctgtatca gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt
    49861 ccagcgacaa gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga
    49921 caatgtgcat cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc
    49981 ggtatccgta tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac
    50041 gacacgttcc aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc
    50101 ttgagtggat aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc
    50161 tggatcagaa acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc
    50221 tgctccatct tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc
    50281 atgttctttt gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg
    50341 gcttcttaaa ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc
    50401 ggtttttaac ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct
    50461 ttttactgtc atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag
    50521 aatcctgtat ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt
    50581 tccgcttttt cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt
    50641 cgagtgatgt ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag
    50701 taatgatgct gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa
    50761 atatgtgtgc catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc
    50821 gagataaaaa gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa
    50881 ttctgtagca gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct
    50941 acaggaatat cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca
    51001 agtactgctc caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact
    51061 aatgtttcag catcataaat atacggtata cgcaattcag gatttttttt catcgtataa
    51121 ataatacctg tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa
    51181 tattcaggta catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct
    51241 tttttaccat acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa
    51301 gcaggcagac gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca
    51361 agcaactgtt ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc
    51421 tcctcctaca acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt
    51481 taaagtcgga tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg
    51541 tccaaatata aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac
    51601 tattataaaa attcagctac acaggccaag taactatagg gaacctactg tttccttttt
    51661 aggtccgcga acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc
    51721 taaacgaatt tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag
    51781 tgcgcgacgg attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc
    51841 gattgcagat ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc
    51901 tttgctcatg atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac
    51961 agtctcatcg attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat
    52021 acgtttttgc atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt
    52081 ttcttctcga atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa
    52141 gtccactttt ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa
    52201 attgaaaacg tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt
    52261 ttgacgaatt ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga
    52321 cccgattaat cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag
    52381 ggcatcttca cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac
    52441 accagttggt ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct
    52501 tcgtgcacgg cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg
    52561 ttcatgcaga agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg
    52621 tagagggaca ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc
    52681 agaataacct tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag
    52741 tgctttttct tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc
    52801 ttccttttct tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg
    52861 atcggtttga tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc
    52921 gggtagttga cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc
    52981 atctgtgatc tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac
    53041 ttctgcttct tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc
    53101 agaatctttt tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa
    53161 ttctcctcga gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc
    53221 acctgcacca attaaggtat gcaattcatc aatgaataag attacttgac catcattata
    53281 gatttcgtcg atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc
    53341 aaccaaagat cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc
    53401 acctaaaatg attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc
    53461 acccaccagt acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac
    53521 ttctctcgaa cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc
    53581 acgtgctaat gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt
    53641 tacatttcta cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg
    53701 tttcagaact tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc
    53761 catgatactt tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga
    53821 accagttttc ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata
    53881 tggaagaaag actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat
    53941 ttcttcataa atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc
    54001 attttgctcg accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa
    54061 ccgttttgct tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc
    54121 ttccatctaa tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat
    54181 ccgagcacgt gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact
    54241 caacatcaag tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat
    54301 agcaaaagca tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa
    54361 ttgtttttta tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt
    54421 gctttctact agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg
    54481 tgaaggaaca cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact
    54541 attttccaga attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat
    54601 cttattcatt ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga
    54661 aagaagaacg ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat
    54721 actattccgt atcgacgcgc ccaagaggag tcgaacccct aaccttttga tccgtagtca
    54781 aacactctat ccagttgagc tatgggcgca tattcaattt aaaaaaatta taaatcattt
    54841 gaaaacaaaa agcaagataa aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg
    54901 gtcccaaacc aagtgctcta ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDOA010000008        52320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDOA010000008 DACDOA010000000
VERSION     DACDOA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.008"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(130..507)
                     /locus_tag="IWI39_000388"
     CDS             complement(130..507)
                     /locus_tag="IWI39_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207518.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            763..1491
                     /locus_tag="IWI39_000389"
     CDS             763..1491
                     /locus_tag="IWI39_000389"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9207519.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1491..1745
                     /gene="purS"
                     /locus_tag="IWI39_000390"
     CDS             1491..1745
                     /gene="purS"
                     /locus_tag="IWI39_000390"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9207520.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1747..2418
                     /gene="purQ"
                     /locus_tag="IWI39_000391"
     CDS             1747..2418
                     /gene="purQ"
                     /locus_tag="IWI39_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9207521.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2419..4641
                     /gene="purL"
                     /locus_tag="IWI39_000392"
     CDS             2419..4641
                     /gene="purL"
                     /locus_tag="IWI39_000392"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9207522.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4626..6065
                     /locus_tag="IWI39_000393"
     CDS             4626..6065
                     /locus_tag="IWI39_000393"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9207523.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6097..7140
                     /locus_tag="IWI39_000394"
     CDS             6097..7140
                     /locus_tag="IWI39_000394"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9207524.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7137..7715
                     /gene="purN"
                     /locus_tag="IWI39_000395"
     CDS             7137..7715
                     /gene="purN"
                     /locus_tag="IWI39_000395"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9207525.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7712..9247
                     /gene="purH"
                     /locus_tag="IWI39_000396"
     CDS             7712..9247
                     /gene="purH"
                     /locus_tag="IWI39_000396"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9207526.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9263..10537
                     /gene="purD"
                     /locus_tag="IWI39_000397"
     CDS             9263..10537
                     /gene="purD"
                     /locus_tag="IWI39_000397"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9207527.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10659..11573
                     /locus_tag="IWI39_000398"
     CDS             10659..11573
                     /locus_tag="IWI39_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9207528.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11706..13271)
                     /locus_tag="IWI39_000399"
     CDS             complement(11706..13271)
                     /locus_tag="IWI39_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9207529.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13264..14715)
                     /locus_tag="IWI39_000400"
     CDS             complement(13264..14715)
                     /locus_tag="IWI39_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9207530.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14729..15745)
                     /locus_tag="IWI39_000401"
     CDS             complement(14729..15745)
                     /locus_tag="IWI39_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9207531.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15965..17053
                     /locus_tag="IWI39_000402"
     CDS             15965..17053
                     /locus_tag="IWI39_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9207532.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17107..18633
                     /locus_tag="IWI39_000403"
     CDS             17107..18633
                     /locus_tag="IWI39_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9207533.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18635..19840
                     /locus_tag="IWI39_000404"
     CDS             18635..19840
                     /locus_tag="IWI39_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207534.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20097..21074
                     /gene="guaC"
                     /locus_tag="IWI39_000405"
     CDS             20097..21074
                     /gene="guaC"
                     /locus_tag="IWI39_000405"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9207535.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21338..22762
                     /locus_tag="IWI39_000406"
     CDS             21338..22762
                     /locus_tag="IWI39_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9207536.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23223..24332
                     /gene="pdhA"
                     /locus_tag="IWI39_000407"
     CDS             23223..24332
                     /gene="pdhA"
                     /locus_tag="IWI39_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9207537.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24335..25312
                     /locus_tag="IWI39_000408"
     CDS             24335..25312
                     /locus_tag="IWI39_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9207538.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25337..26980
                     /locus_tag="IWI39_000409"
     CDS             25337..26980
                     /locus_tag="IWI39_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9207539.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26988..28394
                     /gene="lpdA"
                     /locus_tag="IWI39_000410"
     CDS             26988..28394
                     /gene="lpdA"
                     /locus_tag="IWI39_000410"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9207540.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28556..28831
                     /locus_tag="IWI39_000411"
     CDS             28556..28831
                     /locus_tag="IWI39_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9207541.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28837..29604
                     /locus_tag="IWI39_000412"
     CDS             28837..29604
                     /locus_tag="IWI39_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9207542.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29772..31601
                     /gene="typA"
                     /locus_tag="IWI39_000413"
     CDS             29772..31601
                     /gene="typA"
                     /locus_tag="IWI39_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9207543.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31862..33634
                     /gene="recQ"
                     /locus_tag="IWI39_000414"
     CDS             31862..33634
                     /gene="recQ"
                     /locus_tag="IWI39_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9207544.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33748..34050
                     /locus_tag="IWI39_000415"
     CDS             33748..34050
                     /locus_tag="IWI39_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9207545.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34053..35216
                     /locus_tag="IWI39_000416"
     CDS             34053..35216
                     /locus_tag="IWI39_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9207546.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35242..38670
                     /locus_tag="IWI39_000417"
     CDS             35242..38670
                     /locus_tag="IWI39_000417"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9207547.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38691..39815
                     /locus_tag="IWI39_000418"
     CDS             38691..39815
                     /locus_tag="IWI39_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207548.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39805..40242
                     /locus_tag="IWI39_000419"
     CDS             39805..40242
                     /locus_tag="IWI39_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9207549.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40226..40564
                     /locus_tag="IWI39_000420"
     CDS             40226..40564
                     /locus_tag="IWI39_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9207550.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40683..41243
                     /gene="rsmD"
                     /locus_tag="IWI39_000421"
     CDS             40683..41243
                     /gene="rsmD"
                     /locus_tag="IWI39_000421"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9207551.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41240..41731
                     /gene="coaD"
                     /locus_tag="IWI39_000422"
     CDS             41240..41731
                     /gene="coaD"
                     /locus_tag="IWI39_000422"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9207552.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41721..42764
                     /locus_tag="IWI39_000423"
     CDS             41721..42764
                     /locus_tag="IWI39_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9207553.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42923..43651
                     /locus_tag="IWI39_000424"
     CDS             42923..43651
                     /locus_tag="IWI39_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9207554.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43767..44012
                     /locus_tag="IWI39_000425"
     CDS             43767..44012
                     /locus_tag="IWI39_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9207555.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44009..44227
                     /locus_tag="IWI39_000426"
     CDS             44009..44227
                     /locus_tag="IWI39_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9207556.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44363..44902
                     /locus_tag="IWI39_000427"
     CDS             44363..44902
                     /locus_tag="IWI39_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9207557.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44895..47168
                     /locus_tag="IWI39_000428"
     CDS             44895..47168
                     /locus_tag="IWI39_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9207558.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47206..48252
                     /gene="holA"
                     /locus_tag="IWI39_000429"
     CDS             47206..48252
                     /gene="holA"
                     /locus_tag="IWI39_000429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9207559.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48341..48595)
                     /locus_tag="IWI39_000430"
     CDS             complement(48341..48595)
                     /locus_tag="IWI39_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9207560.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48995..49264)
                     /locus_tag="IWI39_000431"
     CDS             complement(48995..49264)
                     /locus_tag="IWI39_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207561.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49734..50189)
                     /locus_tag="IWI39_000432"
     CDS             complement(49734..50189)
                     /locus_tag="IWI39_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9207562.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50327..52255)
                     /locus_tag="IWI39_000433"
     CDS             complement(50327..52255)
                     /locus_tag="IWI39_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9207563.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17186 a         9002 c        11411 g        14721 t
ORIGIN      
        1 aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa
       61 taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa
      121 atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg
      181 ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat
      241 acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt
      301 gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg
      361 gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat
      421 cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag
      481 ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat
      541 ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata
      601 agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata
      661 tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa
      721 cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt
      781 ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata
      841 gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa
      901 ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata
      961 aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt
     1021 atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt
     1081 gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag
     1141 ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa
     1201 gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt
     1261 gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa
     1321 attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat
     1381 gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa
     1441 gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg
     1501 taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa
     1561 aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg
     1621 agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca
     1681 agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag
     1741 tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta
     1801 tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct
     1861 ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga
     1921 tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa
     1981 gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg
     2041 ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa
     2101 gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca
     2161 gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa
     2221 aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca
     2281 gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta
     2341 gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc
     2401 ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa
     2461 aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat
     2521 atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa
     2581 cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat
     2641 gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt
     2701 gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg
     2761 acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg
     2821 ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa
     2881 gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa
     2941 attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg
     3001 attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat
     3061 gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt
     3121 aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag
     3181 ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata
     3241 caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc
     3301 tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat
     3361 gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa
     3421 gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta
     3481 acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc
     3541 gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc
     3601 gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg
     3661 cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat
     3721 tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt
     3781 agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg
     3841 gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc
     3901 ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa
     3961 gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga
     4021 actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat
     4081 ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag
     4141 gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc agattttgat
     4201 ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat
     4261 ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt
     4321 gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc
     4381 aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa
     4441 actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg
     4501 atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca
     4561 gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca
     4621 atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg
     4681 gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac
     4741 aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg
     4801 ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg
     4861 caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt
     4921 ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg
     4981 ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata
     5041 cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa
     5101 aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa
     5161 tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg
     5221 gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa
     5281 gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct
     5341 ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag
     5401 attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag
     5461 cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag
     5521 ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc
     5581 aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa
     5641 tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt
     5701 caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa
     5761 aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg
     5821 atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat
     5881 gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc
     5941 tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga
     6001 cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct
     6061 tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct
     6121 ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa
     6181 acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg
     6241 gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta
     6301 gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt
     6361 gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg
     6421 gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt
     6481 caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt
     6541 aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca
     6601 ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca
     6661 aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca
     6721 aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat
     6781 gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact
     6841 ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc
     6901 aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt
     6961 cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtccaa
     7021 gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa
     7081 atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga
     7141 gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat
     7201 cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg
     7261 ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg
     7321 attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt
     7381 tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata
     7441 aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac
     7501 gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag
     7561 gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt
     7621 tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta
     7681 gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga
     7741 taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc
     7801 aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga
     7861 agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca
     7921 tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa
     7981 tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa
     8041 accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct
     8101 ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga
     8161 agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact
     8221 agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac
     8281 ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac
     8341 gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga
     8401 aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat
     8461 ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc
     8521 cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga
     8581 agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga
     8641 agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc
     8701 ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac
     8761 agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg
     8821 acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac
     8881 aaaacgtaag ccgactccag cagaagcaaa agcattagcg tttgcttgga gagctgtcaa
     8941 acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc
     9001 cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat
     9061 ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc
     9121 agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc
     9181 tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag
     9241 acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca
     9301 tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg
     9361 aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc
     9421 agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt
     9481 tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc
     9541 gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac
     9601 ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta
     9661 tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg
     9721 tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca
     9781 gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga
     9841 attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga
     9901 tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc
     9961 accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc
    10021 agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt
    10081 gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga
    10141 aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt
    10201 agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt
    10261 tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga
    10321 agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc
    10381 aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa
    10441 aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg
    10501 agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa
    10561 aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg
    10621 tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta
    10681 cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag
    10741 atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag
    10801 atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa
    10861 tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg
    10921 atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg
    10981 attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag
    11041 ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc
    11101 aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc
    11161 agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga
    11221 cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata
    11281 tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga
    11341 acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga
    11401 caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg
    11461 ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt
    11521 ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt
    11581 cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca
    11641 gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc
    11701 gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc
    11761 ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa
    11821 gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt
    11881 tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa
    11941 tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta
    12001 aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa
    12061 ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc
    12121 gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc
    12181 gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga
    12241 tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat
    12301 acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca
    12361 actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa
    12421 ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt
    12481 tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg
    12541 accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact
    12601 attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta
    12661 aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg
    12721 tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca
    12781 agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt
    12841 tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa
    12901 gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga
    12961 taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta
    13021 ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg
    13081 tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa
    13141 gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca
    13201 aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa
    13261 actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac
    13321 agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata
    13381 aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat
    13441 ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt
    13501 gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc
    13561 tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat
    13621 catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat
    13681 atggccgtat cttattttta agatgactag ataatcattg atatggctaa tttctttttc
    13741 aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg
    13801 ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa
    13861 actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc
    13921 taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt
    13981 attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc
    14041 aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac
    14101 ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc
    14161 aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc
    14221 taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga
    14281 atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt
    14341 gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt
    14401 actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc
    14461 ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca
    14521 catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt
    14581 tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa
    14641 agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct
    14701 taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat
    14761 tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt
    14821 gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac
    14881 actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat
    14941 caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta
    15001 aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat
    15061 ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa
    15121 ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg
    15181 gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct
    15241 ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc
    15301 tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga
    15361 aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc
    15421 gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca
    15481 tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt
    15541 agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag
    15601 tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt
    15661 caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg
    15721 ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc
    15781 ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac
    15841 ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa
    15901 aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata
    15961 aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat
    16021 tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat
    16081 cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga
    16141 attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca
    16201 agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat
    16261 cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat
    16321 cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa
    16381 ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg
    16441 aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat
    16501 caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt
    16561 gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc
    16621 gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt
    16681 tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta
    16741 tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt
    16801 taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc
    16861 aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga
    16921 tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc
    16981 tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata aagaatccga
    17041 tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata
    17101 agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc
    17161 cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga
    17221 gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc
    17281 tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat
    17341 agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca
    17401 ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa
    17461 ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat
    17521 cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta
    17581 tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa
    17641 agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt
    17701 atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat
    17761 ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga
    17821 atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt
    17881 tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac
    17941 gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga
    18001 gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc
    18061 ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca
    18121 aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat
    18181 attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat
    18241 aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac
    18301 tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc
    18361 aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat
    18421 gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct
    18481 gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg
    18541 atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg
    18601 atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga
    18661 taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag
    18721 catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt
    18781 ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc
    18841 tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat
    18901 ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat
    18961 gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt
    19021 agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg
    19081 actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa
    19141 aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt
    19201 gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg
    19261 tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt
    19321 tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat
    19381 tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt
    19441 agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat
    19501 caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc
    19561 tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga
    19621 actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac
    19681 aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat
    19741 tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt
    19801 tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga
    19861 aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt
    19921 gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt
    19981 tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt
    20041 gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga
    20101 aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt
    20161 ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg
    20221 ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt
    20281 tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa
    20341 agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag
    20401 agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac
    20461 atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg
    20521 tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag
    20581 atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct
    20641 ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc
    20701 cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg
    20761 gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa
    20821 caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa
    20881 aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga
    20941 aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa
    21001 gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg
    21061 gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt
    21121 aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat
    21181 tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt
    21241 tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa
    21301 atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa
    21361 acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag
    21421 gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa
    21481 tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa
    21541 acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat
    21601 cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat
    21661 aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc
    21721 agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga
    21781 agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt
    21841 ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca
    21901 gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga
    21961 gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg
    22021 gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa
    22081 taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag
    22141 tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga
    22201 agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac
    22261 gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac
    22321 gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga
    22381 aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga
    22441 agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat
    22501 aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta
    22561 tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga
    22621 tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat
    22681 caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct
    22741 agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg
    22801 gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa
    22861 gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca
    22921 aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa
    22981 tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc
    23041 tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt
    23101 atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta
    23161 ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac
    23221 aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag
    23281 atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac
    23341 ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat
    23401 tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg
    23461 ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc
    23521 ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt
    23581 ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac
    23641 caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga
    23701 agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag
    23761 gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata
    23821 tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac
    23881 ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc
    23941 ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag
    24001 tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa
    24061 cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct
    24121 tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg
    24181 aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa
    24241 aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa
    24301 ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc
    24361 aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga
    24421 agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg
    24481 agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg
    24541 tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga
    24601 agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag
    24661 aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca
    24721 ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc
    24781 caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt
    24841 tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta
    24901 cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac
    24961 atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat
    25021 atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca
    25081 atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt
    25141 tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat
    25201 cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt
    25261 gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa
    25321 gggacgaaac aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc
    25381 agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac
    25441 attactagaa gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac
    25501 ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga
    25561 aatcgatgca ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca
    25621 aactccagct aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag
    25681 cgaaggagtc ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat
    25741 cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt
    25801 tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt
    25861 gattgttcca gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc
    25921 tggacacaat agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt
    25981 ggaaacatca ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc
    26041 tatgccatct gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc
    26101 aactggaaaa ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc
    26161 aagcagtgca ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc
    26221 tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt
    26281 ggaagaacgt gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag
    26341 taagcataca gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga
    26401 taatcggaaa cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc
    26461 atatgtcgtg aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat
    26521 cgacgatgca aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga
    26581 tacggatcac ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc
    26641 tattgcagac gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga
    26701 agatatgcgc aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt
    26761 tacaccagtc atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca
    26821 accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag
    26881 tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg
    26941 tcttttagct gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag
    27001 attttgctgt agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg
    27061 ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg
    27121 gtgtttgttt gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt
    27181 atcaagagtc actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt
    27241 ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg
    27301 gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg
    27361 atcacacact acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa
    27421 tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt
    27481 tagattctac tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg
    27541 gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga
    27601 ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag
    27661 aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag
    27721 tcgataacgg tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta
    27781 ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag
    27841 aacaagcggg tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta
    27901 cgaacgtacc taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca
    27961 aagccagtta tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag
    28021 attataaagc aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga
    28081 cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag
    28141 ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga
    28201 tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct
    28261 ctgaattagc tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc
    28321 atcctcatcc atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac
    28381 caatccatat ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt
    28441 gttttttaga attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt
    28501 ttattaccac ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca
    28561 agaatatcag tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa
    28621 gatgtgggaa gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa
    28681 gacttatcag cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg
    28741 ggaatttgaa aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa
    28801 tccaaacaaa actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc
    28861 aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc
    28921 tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt
    28981 ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg
    29041 tgtaatcaat tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc
    29101 ttgaattttg tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc
    29161 gttggaaagc ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa
    29221 ggaataggcg tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg
    29281 gaaggcttac ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa
    29341 attgcggaaa gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca
    29401 atcttgaaag gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg
    29461 ggaaatgtct tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg
    29521 acatttgaaa aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca
    29581 gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa
    29641 cgagcaaaag attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa
    29701 gtctgctata attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg
    29761 agttagttac attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg
    29821 accacggaaa aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac
    29881 atacgcaatt gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca
    29941 cgattttagc caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata
    30001 ctccaggaca cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg
    30061 ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa
    30121 aagcactgga acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg
    30181 ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg
    30241 atgatcagtt agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt
    30301 cagatgatcc agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa
    30361 aaatccctgc tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg
    30421 actacaacga ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag
    30481 taggagacca agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga
    30541 aattatttgg tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact
    30601 tgattgctgt ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc
    30661 atcaagatgc gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag
    30721 ttaataattc accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag
    30781 aacgtttgat ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc
    30841 cagatgcatg gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca
    30901 tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg
    30961 acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg
    31021 gtagtgttat cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag
    31081 gaaacggaca aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa
    31141 ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc
    31201 tgccaatgct tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata
    31261 caggaaaagc aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag
    31321 aaccaggtac agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt
    31381 tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc
    31441 aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga
    31501 atgatgatga atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag
    31561 agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag
    31621 agaaaccagc ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg
    31681 agaagtcatc tttgtcaatg gacataaatt cagaaaatac gccgaaagac gtttaaaaga
    31741 cttttaaaaa acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa
    31801 ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa
    31861 gatgaacgac ttattaaaac aatactttgg ctatgatgag tttagacctg gtcaaaaaga
    31921 aattatccag aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg
    31981 gaaatcaatc tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc
    32041 ccctttaatc tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc
    32101 cacttttatc aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga
    32161 tagacagcag taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat
    32221 ccaagcaatg aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc
    32281 tcagtggggg catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact
    32341 gccaaatcgt cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga
    32401 cataaaaaga cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga
    32461 aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt
    32521 aaagatcaac aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg
    32581 tctctatcat ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa
    32641 tgagaatgag cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt
    32701 agcgacaaat gcatttggta tgggtataaa taaaagcaat gtccgttttg ttatccatta
    32761 tcaaattcct ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg
    32821 tctttccagt gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt
    32881 tatccagcag tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc
    32941 gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg
    33001 cgagacaagc agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga
    33061 agtaacgaca gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg
    33121 taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg
    33181 ttttggacat ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca
    33241 gttaattgaa tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt
    33301 tttgaaagta acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa
    33361 ggaacccaag aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct
    33421 gcgggaactg cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc
    33481 agattccaca ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca
    33541 gataaaagga gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat
    33601 aaaaaatgcg gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata
    33661 aaaacaactg gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta
    33721 aggaattaga atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa
    33781 gtattaaatg atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc
    33841 tgtatctcac aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc
    33901 cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg
    33961 ttaagtgagc tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat
    34021 gataaaaaag aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc
    34081 tattagacta tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg
    34141 tatatagttc tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca
    34201 ttaaccagag tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga
    34261 aaacagatgt tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt
    34321 tgctgctgat tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa
    34381 ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt
    34441 ctctgacttt atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac
    34501 gtccacttgc aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta
    34561 atgcggcagt tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg
    34621 tgtatacttt gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga
    34681 tcaatatcac aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg
    34741 ctatttacca aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct
    34801 actatgccat gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa
    34861 aaggattttt acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg
    34921 gagtgattat ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc
    34981 ttgtaggtat ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa
    35041 tgtttttgat ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag
    35101 ggatcacgtt cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg
    35161 tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa
    35221 aaaagatgta ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct
    35281 gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa
    35341 gaagatgaat attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga
    35401 aaaaaaccga ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc
    35461 ggagctgacg ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa
    35521 cggtgcgaag aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt
    35581 ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc
    35641 acagatggac caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat
    35701 ccgattatga tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat
    35761 gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt
    35821 agtgatgaag tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt
    35881 ctcggggata cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc
    35941 cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa
    36001 aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca
    36061 gtagaatttc ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa
    36121 gtggaacata cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg
    36181 attgctcaag gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa
    36241 ttaaatggtt cagccattca atgccgaatc actacagaag acccgttgaa taacttttta
    36301 ccagacacag ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat
    36361 gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa
    36421 gtatgtacgc atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa
    36481 gaatttagaa tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat
    36541 cctgaattcc aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt
    36601 gaatttcctc gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact
    36661 gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg
    36721 cctaaagatc taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag
    36781 ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg
    36841 acatttagag acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag
    36901 cagattgcac gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg
    36961 ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga
    37021 aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc
    37081 gttgggtatt ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg
    37141 caaggaatcg atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa
    37201 agtatccaag ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc
    37261 gatatcaacg atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag
    37321 gaactagaac aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa
    37381 ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat
    37441 ctgcatactc atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca
    37501 ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc
    37561 atgagcagct tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa
    37621 aatgcacaga aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa
    37681 aacggcttga atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat
    37741 tcgaatctac agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa
    37801 caaatgtatc atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct
    37861 aaagtagttg gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt
    37921 tatgaaaaag gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa
    37981 ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga
    38041 cctgcattga atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa
    38101 aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg
    38161 atgtatccgc aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca
    38221 ttactggaca caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa
    38281 atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa
    38341 gggaatcgcg tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat
    38401 aattcgatca tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa
    38461 attggagcaa cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt
    38521 aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca
    38581 cgttttgacg gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt
    38641 gaccttttga ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac
    38701 tgggtttgtt ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct
    38761 tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga
    38821 caacgtggaa ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga
    38881 tcgatcagct ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt
    38941 ttgaaacaag atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag
    39001 acagcagagt cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt
    39061 atttagggat gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt
    39121 ttgagtacca tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc
    39181 cgctaattgc ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg
    39241 gcctattttc agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag
    39301 tgtttggtga aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca
    39361 atcgcgttaa agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac
    39421 ttcccatata caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt
    39481 ttttgacaag gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc
    39541 taggcagtgc tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa
    39601 tcagtaaaca aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta
    39661 ctttttcgtt tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg
    39721 gaacggattc tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag
    39781 aaaacggaga aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat
    39841 caatgcctcc aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa
    39901 cataaacagt caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct
    39961 aagaaagaag catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa
    40021 aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct
    40081 ttccgatata gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc
    40141 gaagaagttt ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa
    40201 cactttggat gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa
    40261 acgacgcggg ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt
    40321 cggcttgttg cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga
    40381 gacattggaa gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg
    40441 ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt
    40501 aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct
    40561 ttaaaaatct cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt
    40621 gatcattgtc caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt
    40681 agatgcggat aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga
    40741 acaccagacc aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt
    40801 ttgatggaga gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag
    40861 tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca
    40921 tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg
    40981 cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg
    41041 atccgccata tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac
    41101 ttttttctaa aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa
    41161 ctgtggctgg attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct
    41221 atagaaaaga gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac
    41281 aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg
    41341 tgtttttatc aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat
    41401 cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac
    41461 ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa
    41521 agactttgaa tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga
    41581 aactgtcttc ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga
    41641 agtactgcac tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt
    41701 agctggaaag cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca
    41761 tatttggaat gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac
    41821 cgggagccac gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt
    41881 ctgggtcttt ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa
    41941 aagctaaaat gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga
    42001 caaatgaaga atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa
    42061 tcgaacaagc gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg
    42121 tcatgagtgt agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa
    42181 caaaagtaga cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc
    42241 aaaaagttgg acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt
    42301 ctggtgatct gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg
    42361 accataccga gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc
    42421 catcagcggg attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc
    42481 gacatggtaa gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa
    42541 tcggaggaat cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg
    42601 caccagatga tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact
    42661 acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa
    42721 aaacagtaca agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag
    42781 aggggttgtg acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa
    42841 aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag
    42901 ctcactgttt cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt
    42961 tgaaaaaaga acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat
    43021 catttggatg tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa
    43081 tttgctattt tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag
    43141 ttccagtgga gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa
    43201 ggtcatgtac accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc
    43261 agaagaacgg gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag
    43321 cgatcgaatc aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt
    43381 aggtgaagag ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac
    43441 agcagattcc gagcctccca aaataaatat caatacagcc actccatcag aacttcagca
    43501 gattcctggg atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg
    43561 atccttccag aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga
    43621 aaaactaaaa aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa
    43681 taaatggacg ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga
    43741 ggtagtattt ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa
    43801 tgctgaatgt ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca
    43861 gcggcaataa cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag
    43921 cagataaagt atttattctc attgggacga atgatctagc agagcataaa caactttcta
    43981 aagaaacgta ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc
    44041 tgatcatgct ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa
    44101 taacaagttg atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg
    44161 tgaagtatcg atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat
    44221 gggctaatgg atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg
    44281 atgattcgtt atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg
    44341 aaaaaacgga ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa
    44401 agaaaaagtt actgataagt cacataatga acgcattcct tgggaccagt actttatggc
    44461 tcaggcagta ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact
    44521 cgttcgagaa aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca
    44581 ctgcatagat gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga
    44641 gatgaatgca ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta
    44701 tgtcacacat tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg
    44761 gatcaattat ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt
    44821 aggtgtatct gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga
    44881 attaacagga aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc
    44941 cggcaattac gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt
    45001 tgcttcttgc cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact
    45061 tgtgtatttt gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt
    45121 tagaggaaaa aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg
    45181 gtaatttttt gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt
    45241 atcaagtaaa cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag
    45301 aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg
    45361 accaagaagc ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg
    45421 aacagatcac catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta
    45481 tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat
    45541 ggggctataa agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc
    45601 tgcatctgtt cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt
    45661 tgtttcgacg tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta
    45721 tctgttatgt ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt
    45781 tattgcgatt gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag
    45841 ttgtcttttg gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc
    45901 ttagtttttt catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa
    45961 aaacggcctc tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt
    46021 atgagtggcc tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc
    46081 tttttcttcc tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac
    46141 ttttgctgat agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt
    46201 cgtctctcat tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac
    46261 tgatttgtat ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt
    46321 tttcttttat ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg
    46381 atgtcggtca aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga
    46441 tcgatactgg aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc
    46501 ctcctgctga aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata
    46561 aactcatctt gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc
    46621 attttactat caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa
    46681 ggcatttatc tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca
    46741 ggatcagcgg ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa
    46801 atgaagattc gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag
    46861 atttgaatca agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg
    46921 tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata
    46981 ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca
    47041 gcgaagtatt ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag
    47101 gaatgatcac ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga
    47161 tagactagta attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg
    47221 agtatacaag cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt
    47281 ttaggaacag aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa
    47341 ttagagggcg aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat
    47401 gaggtagtag ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta
    47461 gagaatcctt attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat
    47521 ttattgacgg cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca
    47581 tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc
    47641 atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag
    47701 aattcctcaa tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta
    47761 gatttatcca aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag
    47821 caaatcacgg taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt
    47881 gacatgaccc aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat
    47941 ttagttatgc aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt
    48001 ttatttttac agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa
    48061 acattgaaaa tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat
    48121 gaacatttgc tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg
    48181 ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta
    48241 agaaatcgat aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc
    48301 tttttttgac aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc
    48361 ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca
    48421 tatcaatagc tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta
    48481 cagcatcttc aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca
    48541 cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt
    48601 tcacctccga taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat
    48661 acctaaaggc agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt
    48721 tattaggatt ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc
    48781 tattgtggga ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag
    48841 tttgaatggg aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt
    48901 ttttttgaaa agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt
    48961 tattttataa ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa
    49021 tgtattcatc gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc
    49081 atttgccact tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca
    49141 aatagcgatc tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag
    49201 tcagcatcat tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt
    49261 ccacaagcgt cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt
    49321 tgatattcgt tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac
    49381 aaaaaagagc agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt
    49441 ggcacggcct aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat
    49501 cccctatatc gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat
    49561 caggaaataa tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga
    49621 ttgttcctat gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca
    49681 cggatcagta aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt
    49741 cctaaagcaa atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg
    49801 gcgattcctc ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt
    49861 tgtggatcag aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg
    49921 caaaaaacag ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg
    49981 atttctcctg ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg
    50041 attcctttgc cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc
    50101 aaaacaaaag ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa
    50161 ataattccta aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat
    50221 cataacatgt ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa
    50281 gctgtttttt tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag
    50341 acagccgcgc tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag
    50401 gtaaaggtct tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc
    50461 ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa
    50521 taacaagaat cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc
    50581 gatgactgta gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca
    50641 tagcggcatc tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg
    50701 catcattgac tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga
    50761 ctagttgtgt tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt
    50821 cagcaatggc ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt
    50881 gggatttaaa gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta
    50941 aaacagcaat taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca
    51001 aacgttcgat catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt
    51061 gcttgccaac tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt
    51121 cttctattgg taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg
    51181 catgagcaag tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat
    51241 ccttttcatc aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg
    51301 ttttatcaaa ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga
    51361 ttaaaatccc attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg
    51421 cgacaagcgc acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat
    51481 cccaactcca acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa
    51541 tcaattttac ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat
    51601 tctgcgcttc ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg
    51661 ttactcgaat cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta
    51721 aatatttttc tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg
    51781 tgctttctaa aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat
    51841 ccacctttag ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg
    51901 ctttttgtgg ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt
    51961 attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat
    52021 taccgatcaa acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc
    52081 gatcattcca caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg
    52141 cactgatata aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga
    52201 tcataaataa aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta
    52261 ttagacctcc ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag
//
LOCUS       DACDOA010000009        51903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDOA010000009 DACDOA010000000
VERSION     DACDOA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.009"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            163..501
                     /locus_tag="IWI39_000434"
     CDS             163..501
                     /locus_tag="IWI39_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207564.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            595..825
                     /locus_tag="IWI39_000435"
     CDS             595..825
                     /locus_tag="IWI39_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207565.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            1225..1596
                     /locus_tag="IWI39_000436"
     CDS             1225..1596
                     /locus_tag="IWI39_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9207566.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(1870..2562)
                     /locus_tag="IWI39_000437"
     CDS             complement(1870..2562)
                     /locus_tag="IWI39_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9207567.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(2565..3350)
                     /locus_tag="IWI39_000438"
     CDS             complement(2565..3350)
                     /locus_tag="IWI39_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9207568.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            3587..4987
                     /gene="lacG"
                     /locus_tag="IWI39_000439"
     CDS             3587..4987
                     /gene="lacG"
                     /locus_tag="IWI39_000439"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9207569.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5012..5329
                     /locus_tag="IWI39_000440"
     CDS             5012..5329
                     /locus_tag="IWI39_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9207570.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            5342..7015
                     /locus_tag="IWI39_000441"
     CDS             5342..7015
                     /locus_tag="IWI39_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207571.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            7029..8660
                     /locus_tag="IWI39_000442"
     CDS             7029..8660
                     /locus_tag="IWI39_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9207572.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(9151..10716)
                     /gene="guaA"
                     /locus_tag="IWI39_000443"
     CDS             complement(9151..10716)
                     /gene="guaA"
                     /locus_tag="IWI39_000443"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9207573.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10775..11698)
                     /locus_tag="IWI39_000444"
     CDS             complement(10775..11698)
                     /locus_tag="IWI39_000444"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9207574.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11894..12499
                     /locus_tag="IWI39_000445"
     CDS             11894..12499
                     /locus_tag="IWI39_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9207575.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12807..13823
                     /locus_tag="IWI39_000446"
     CDS             12807..13823
                     /locus_tag="IWI39_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207576.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13964..15265
                     /locus_tag="IWI39_000447"
     CDS             13964..15265
                     /locus_tag="IWI39_000447"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9207577.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15311..15973
                     /gene="deoC"
                     /locus_tag="IWI39_000448"
     CDS             15311..15973
                     /gene="deoC"
                     /locus_tag="IWI39_000448"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9207578.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15994..16392
                     /locus_tag="IWI39_000449"
     CDS             15994..16392
                     /locus_tag="IWI39_000449"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9207579.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16458..17540
                     /locus_tag="IWI39_000450"
     CDS             16458..17540
                     /locus_tag="IWI39_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9207580.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17752..19314
                     /locus_tag="IWI39_000451"
     CDS             17752..19314
                     /locus_tag="IWI39_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207581.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19307..20440
                     /locus_tag="IWI39_000452"
     CDS             19307..20440
                     /locus_tag="IWI39_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207582.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20440..21396
                     /locus_tag="IWI39_000453"
     CDS             20440..21396
                     /locus_tag="IWI39_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207583.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21495..22661
                     /gene="deoB"
                     /locus_tag="IWI39_000454"
     CDS             21495..22661
                     /gene="deoB"
                     /locus_tag="IWI39_000454"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9207584.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22685..23503
                     /locus_tag="IWI39_000455"
     CDS             22685..23503
                     /locus_tag="IWI39_000455"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9207585.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23520..24227
                     /gene="deoD"
                     /locus_tag="IWI39_000456"
     CDS             23520..24227
                     /gene="deoD"
                     /locus_tag="IWI39_000456"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9207586.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24525..25211)
                     /gene="gpmA"
                     /locus_tag="IWI39_000457"
     CDS             complement(24525..25211)
                     /gene="gpmA"
                     /locus_tag="IWI39_000457"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9207587.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25383..25964)
                     /locus_tag="IWI39_000458"
     CDS             complement(25383..25964)
                     /locus_tag="IWI39_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9207588.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26097..26780)
                     /gene="rpiA"
                     /locus_tag="IWI39_000459"
     CDS             complement(26097..26780)
                     /gene="rpiA"
                     /locus_tag="IWI39_000459"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9207589.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27099..27512
                     /gene="rpsL"
                     /locus_tag="IWI39_000460"
     CDS             27099..27512
                     /gene="rpsL"
                     /locus_tag="IWI39_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9207590.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27596..28066
                     /gene="rpsG"
                     /locus_tag="IWI39_000461"
     CDS             27596..28066
                     /gene="rpsG"
                     /locus_tag="IWI39_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9207591.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28160..30247
                     /gene="fusA"
                     /locus_tag="IWI39_000462"
     CDS             28160..30247
                     /gene="fusA"
                     /locus_tag="IWI39_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9207592.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30419..31606
                     /gene="tuf"
                     /locus_tag="IWI39_000463"
     CDS             30419..31606
                     /gene="tuf"
                     /locus_tag="IWI39_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9207593.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            32013..32321
                     /gene="rpsJ"
                     /locus_tag="IWI39_000464"
     CDS             32013..32321
                     /gene="rpsJ"
                     /locus_tag="IWI39_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9207594.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            32352..32981
                     /gene="rplC"
                     /locus_tag="IWI39_000465"
     CDS             32352..32981
                     /gene="rplC"
                     /locus_tag="IWI39_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9207595.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            33009..33632
                     /gene="rplD"
                     /locus_tag="IWI39_000466"
     CDS             33009..33632
                     /gene="rplD"
                     /locus_tag="IWI39_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9207596.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            33632..33922
                     /gene="rplW"
                     /locus_tag="IWI39_000467"
     CDS             33632..33922
                     /gene="rplW"
                     /locus_tag="IWI39_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9207597.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33961..34794
                     /gene="rplB"
                     /locus_tag="IWI39_000468"
     CDS             33961..34794
                     /gene="rplB"
                     /locus_tag="IWI39_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9207598.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34837..35115
                     /gene="rpsS"
                     /locus_tag="IWI39_000469"
     CDS             34837..35115
                     /gene="rpsS"
                     /locus_tag="IWI39_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9207599.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            35138..35485
                     /gene="rplV"
                     /locus_tag="IWI39_000470"
     CDS             35138..35485
                     /gene="rplV"
                     /locus_tag="IWI39_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9207600.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            35499..36155
                     /gene="rpsC"
                     /locus_tag="IWI39_000471"
     CDS             35499..36155
                     /gene="rpsC"
                     /locus_tag="IWI39_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9207601.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            36158..36592
                     /gene="rplP"
                     /locus_tag="IWI39_000472"
     CDS             36158..36592
                     /gene="rplP"
                     /locus_tag="IWI39_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9207602.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            36582..36770
                     /gene="rpmC"
                     /locus_tag="IWI39_000473"
     CDS             36582..36770
                     /gene="rpmC"
                     /locus_tag="IWI39_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9207603.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36795..37061
                     /gene="rpsQ"
                     /locus_tag="IWI39_000474"
     CDS             36795..37061
                     /gene="rpsQ"
                     /locus_tag="IWI39_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9207604.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            37119..37487
                     /gene="rplN"
                     /locus_tag="IWI39_000475"
     CDS             37119..37487
                     /gene="rplN"
                     /locus_tag="IWI39_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9207605.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            37524..37832
                     /gene="rplX"
                     /locus_tag="IWI39_000476"
     CDS             37524..37832
                     /gene="rplX"
                     /locus_tag="IWI39_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9207606.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37860..38399
                     /gene="rplE"
                     /locus_tag="IWI39_000477"
     CDS             37860..38399
                     /gene="rplE"
                     /locus_tag="IWI39_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9207607.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            38418..38603
                     /locus_tag="IWI39_000478"
     CDS             38418..38603
                     /locus_tag="IWI39_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9207608.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            38639..39037
                     /gene="rpsH"
                     /locus_tag="IWI39_000479"
     CDS             38639..39037
                     /gene="rpsH"
                     /locus_tag="IWI39_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9207609.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            39069..39605
                     /gene="rplF"
                     /locus_tag="IWI39_000480"
     CDS             39069..39605
                     /gene="rplF"
                     /locus_tag="IWI39_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9207610.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39766..40122
                     /gene="rplR"
                     /locus_tag="IWI39_000481"
     CDS             39766..40122
                     /gene="rplR"
                     /locus_tag="IWI39_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9207611.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            40143..40643
                     /gene="rpsE"
                     /locus_tag="IWI39_000482"
     CDS             40143..40643
                     /gene="rpsE"
                     /locus_tag="IWI39_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9207612.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40658..40837
                     /gene="rpmD"
                     /locus_tag="IWI39_000483"
     CDS             40658..40837
                     /gene="rpmD"
                     /locus_tag="IWI39_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9207613.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40881..41321
                     /gene="rplO"
                     /locus_tag="IWI39_000484"
     CDS             40881..41321
                     /gene="rplO"
                     /locus_tag="IWI39_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9207614.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            41321..42616
                     /gene="secY"
                     /locus_tag="IWI39_000485"
     CDS             41321..42616
                     /gene="secY"
                     /locus_tag="IWI39_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9207615.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42678..43325
                     /locus_tag="IWI39_000486"
     CDS             42678..43325
                     /locus_tag="IWI39_000486"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9207616.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            43516..43734
                     /gene="infA"
                     /locus_tag="IWI39_000487"
     CDS             43516..43734
                     /gene="infA"
                     /locus_tag="IWI39_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9207617.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43767..43883
                     /gene="rpmJ"
                     /locus_tag="IWI39_000488"
     CDS             43767..43883
                     /gene="rpmJ"
                     /locus_tag="IWI39_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9207618.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43901..44266
                     /gene="rpsM"
                     /locus_tag="IWI39_000489"
     CDS             43901..44266
                     /gene="rpsM"
                     /locus_tag="IWI39_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9207619.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            44294..44683
                     /gene="rpsK"
                     /locus_tag="IWI39_000490"
     CDS             44294..44683
                     /gene="rpsK"
                     /locus_tag="IWI39_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9207620.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44763..45701
                     /locus_tag="IWI39_000491"
     CDS             44763..45701
                     /locus_tag="IWI39_000491"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9207621.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45729..46109
                     /gene="rplQ"
                     /locus_tag="IWI39_000492"
     CDS             45729..46109
                     /gene="rplQ"
                     /locus_tag="IWI39_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9207622.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            46381..47220
                     /locus_tag="IWI39_000493"
     CDS             46381..47220
                     /locus_tag="IWI39_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9207623.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            47196..48065
                     /locus_tag="IWI39_000494"
     CDS             47196..48065
                     /locus_tag="IWI39_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9207624.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            48055..48852
                     /locus_tag="IWI39_000495"
     CDS             48055..48852
                     /locus_tag="IWI39_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9207625.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            49055..49801
                     /gene="truA"
                     /locus_tag="IWI39_000496"
     CDS             49055..49801
                     /gene="truA"
                     /locus_tag="IWI39_000496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9207626.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            50466..50703
                     /locus_tag="IWI39_000497"
                     /pseudo
     CDS             50466..50703
                     /locus_tag="IWI39_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(50847..51623)
                     /locus_tag="IWI39_000498"
     CDS             complement(50847..51623)
                     /locus_tag="IWI39_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9207627.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            51746..>51903
                     /locus_tag="IWI39_000499"
     CDS             51746..>51903
                     /locus_tag="IWI39_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9207628.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTV"
BASE COUNT        16501 a         9380 c        11247 g        14775 t
ORIGIN      
        1 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
       61 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
      121 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
      181 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
      241 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
      301 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
      361 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
      421 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
      481 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
      541 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
      601 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
      661 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
      721 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
      781 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
      841 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
      901 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
      961 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
     1021 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
     1081 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
     1141 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
     1201 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
     1261 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
     1321 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
     1381 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
     1441 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
     1501 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
     1561 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
     1621 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
     1681 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
     1741 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
     1801 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
     1861 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
     1921 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
     1981 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
     2041 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
     2101 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
     2161 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
     2221 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
     2281 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
     2341 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
     2401 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
     2461 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
     2521 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
     2581 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
     2641 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
     2701 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
     2761 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
     2821 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
     2881 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
     2941 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
     3001 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
     3061 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
     3121 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
     3181 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
     3241 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
     3301 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
     3361 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
     3421 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
     3481 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
     3541 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
     3601 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
     3661 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
     3721 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
     3781 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
     3841 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
     3901 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
     3961 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
     4021 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
     4081 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
     4141 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
     4201 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
     4261 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
     4321 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
     4381 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
     4441 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
     4501 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
     4561 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
     4621 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
     4681 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
     4741 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
     4801 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
     4861 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
     4921 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
     4981 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
     5041 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
     5101 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
     5161 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
     5221 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
     5281 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
     5341 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
     5401 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
     5461 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
     5521 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
     5581 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
     5641 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
     5701 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
     5761 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
     5821 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
     5881 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
     5941 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
     6001 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
     6061 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
     6121 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
     6181 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
     6241 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
     6301 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
     6361 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
     6421 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
     6481 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
     6541 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
     6601 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
     6661 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
     6721 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
     6781 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
     6841 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
     6901 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
     6961 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
     7021 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
     7081 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
     7141 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
     7201 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
     7261 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
     7321 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
     7381 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
     7441 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
     7501 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
     7561 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
     7621 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
     7681 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
     7741 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
     7801 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
     7861 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
     7921 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
     7981 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
     8041 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
     8101 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
     8161 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
     8221 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
     8281 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
     8341 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
     8401 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
     8461 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
     8521 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
     8581 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
     8641 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
     8701 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
     8761 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
     8821 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
     8881 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
     8941 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
     9001 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
     9061 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
     9121 gggattcccc ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt
     9181 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
     9241 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
     9301 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
     9361 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
     9421 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
     9481 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
     9541 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
     9601 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
     9661 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
     9721 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
     9781 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
     9841 gtctaagaag cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag
     9901 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
     9961 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
    10021 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
    10081 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
    10141 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
    10201 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
    10261 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
    10321 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
    10381 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
    10441 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
    10501 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
    10561 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
    10621 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
    10681 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
    10741 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
    10801 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
    10861 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
    10921 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    10981 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    11041 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    11101 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    11161 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    11221 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    11281 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    11341 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    11401 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    11461 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    11521 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    11581 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    11641 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    11701 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    11761 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    11821 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    11881 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    11941 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    12001 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    12061 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    12121 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    12181 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    12241 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    12301 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    12361 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    12421 aatggaagaa acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat
    12481 caaaagtgtg aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg
    12541 cgtgcatgta cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa
    12601 ctattttttc ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa
    12661 ataagataca gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca
    12721 aaaaacatac tttccttttt tctctattct tttcttgggg gttttggtac actgataggg
    12781 aatttagata gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg
    12841 caggcgtatc agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg
    12901 ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt
    12961 tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca
    13021 ctgatttctt cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct
    13081 atgtgatcat gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag
    13141 agctttccca ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga
    13201 aatatgcatt agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat
    13261 tgaatcgtcg ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg
    13321 tttcgctgtt tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat
    13381 actatcaatt agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc
    13441 cttttaatgg gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat
    13501 caaaaaaatt actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg
    13561 cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag
    13621 tcataggatt cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc
    13681 agcagccaag ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat
    13741 ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg
    13801 taaaagtatt gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg
    13861 ctagttagtg ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta
    13921 aaaataatgt ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct
    13981 gattgaaaaa aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac
    14041 gaactatacg aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt
    14101 ttatcaagac atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg
    14161 tgatgtcatc gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg
    14221 cgttggtgac acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt
    14281 ggctaaaatg tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc
    14341 aattcccgga tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag
    14401 taaagtagcg gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc
    14461 cttgcgtgac gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag
    14521 taaaaaaatc gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc
    14581 tttcatgaag aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa
    14641 actggctaac cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc
    14701 tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga
    14761 agatctagta gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc
    14821 taagacaacc gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt
    14881 agcaaaattc aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc
    14941 agaacgaatt ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt
    15001 gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa
    15061 aaccaaagaa gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga
    15121 cactgtgaca aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc
    15181 tgtcattgaa ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt
    15241 gattcatgac atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag
    15301 gggaaaagaa atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc
    15361 taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg
    15421 catcaatcca gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt
    15481 atgtacagta atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac
    15541 aacagatgcc atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt
    15601 gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa
    15661 agatcgtgcg cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat
    15721 ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt
    15781 ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga
    15841 aatgggtgtc aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga
    15901 agctggtgct acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg
    15961 tgaaggctat taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc
    16021 aacacagcta ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc
    16081 gcttgtctcg taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca
    16141 tttggattaa cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa
    16201 aaaacattta cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt
    16261 gcatgtcgac aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga
    16321 gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa
    16381 aaagatctat aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt
    16441 taggaggctt tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag
    16501 cagcagggtt atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca
    16561 aaagctcatc agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc
    16621 gttcattcaa ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt
    16681 cacgaggaaa tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa
    16741 atattgacca agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa
    16801 aaccagcgat ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg
    16861 atgtcatcga tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt
    16921 atcttgcagg tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg
    16981 gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag
    17041 gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg
    17101 ctccagataa aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct
    17161 tccatgcttc aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat
    17221 caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat
    17281 acacattaaa cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa
    17341 cagtagtaga agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca
    17401 ctgtttatgg attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag
    17461 cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag
    17521 aaactcctga agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg
    17581 ctatggtttg attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga
    17641 taagggaaca actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga
    17701 atcttagtca atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa
    17761 gaaaatattg tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat
    17821 gacaacatca atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt
    17881 gctggcaaat ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa
    17941 attttgatgc gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg
    18001 attgggatgg tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc
    18061 gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag
    18121 atccaaaaag tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat
    18181 atttcagttg gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat
    18241 gtactgattt ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc
    18301 gtgatcatga aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta
    18361 gatgaaatca aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt
    18421 acagtcaacg ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg
    18481 gtttcattca aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa
    18541 aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt
    18601 cgtgctggcg aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc
    18661 caagctttga ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac
    18721 atcactaata aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac
    18781 cgtcataaat acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc
    18841 tatcatcaaa aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat
    18901 gccagagaat tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa
    18961 gctttatcag gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct
    19021 gatctgttga ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt
    19081 cataaacgtc tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa
    19141 ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc
    19201 ggtatcgttg atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat
    19261 accttagaag aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag
    19321 cgaaaaattc cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg
    19381 cgcgatattg atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga
    19441 tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat
    19501 ttttacagca ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc
    19561 tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct
    19621 gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc
    19681 agcggctgtt ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat
    19741 catgatgaac tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga
    19801 aagctatcgc tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt
    19861 accatggtta acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat
    19921 catcgcgctt attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg
    19981 ttctgttgga ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat
    20041 cgttttgtcg atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt
    20101 aggaacgtat caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat
    20161 ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt
    20221 cagtattttg aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat
    20281 cgtcaatgta tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt
    20341 cgtcatggct aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc
    20401 gaaaccaacg aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt
    20461 gccttgatcc ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc
    20521 ggaacttttt ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg
    20581 ggtgctttta gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca
    20641 ccatggatcg ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca
    20701 gtagcaacag tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg
    20761 gcacctgctt taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac
    20821 atcacgcaga atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga
    20881 ccaattttct ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta
    20941 gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat
    21001 cctcaagctg ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg
    21061 atttctggct tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac
    21121 ttttcagctg gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt
    21181 aagtggaatc ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg
    21241 agtatcattg gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt
    21301 gctccatatt tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca
    21361 aaagcaaacg ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg
    21421 gttacgggaa gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga
    21481 aagaaggaat aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg
    21541 gagaagcacc agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg
    21601 cgaaagaagc aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc
    21661 ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag
    21721 tttctgtagg gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa
    21781 ctccatttcg tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat
    21841 tttctggccg taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt
    21901 acggtgaaca ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc
    21961 ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat
    22021 tcgttcgtga tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt
    22081 acgtgggtga accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc
    22141 cattcggaca cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg
    22201 gaaagatcaa cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca
    22261 atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct
    22321 ttaccaattt agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg
    22381 cgcatgcgat tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag
    22441 acgacttgct aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc
    22501 atacgcgaga atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat
    22561 tgcctcaagg atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt
    22621 cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa
    22681 gcaaatgacc aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg
    22741 agtaaaagaa attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga
    22801 aatcgaagat gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt
    22861 agttgggcat gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat
    22921 gcaagggaga ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg
    22981 tgtgatggcc ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa
    23041 cgaatcattt gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga
    23101 aaatcctttg atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca
    23161 agcttatacg cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga
    23221 tcttaaagaa ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat
    23281 ccgaatgtct cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat
    23341 cgtagcagct cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc
    23401 aggtatgcaa gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca
    23461 atcttttaaa gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa
    23521 tgagtgtgca tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag
    23581 atcctttgcg agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc
    23641 aggtacgcgg tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag
    23701 gaacaggaat gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg
    23761 gtgtaaagaa attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc
    23821 gtgatttagt actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc
    23881 aagcctttca ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag
    23941 ctaaagaaaa gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca
    24001 aagatgattt gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg
    24061 aagcagcggc tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga
    24121 ccgttagcga ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt
    24181 tcaatgaaat gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa
    24241 aagctgccct gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga
    24301 cttggctgga cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg
    24361 aatctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
    24421 atgaatattg tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag
    24481 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
    24541 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
    24601 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
    24661 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
    24721 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
    24781 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
    24841 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
    24901 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
    24961 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
    25021 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
    25081 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
    25141 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
    25201 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
    25261 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
    25321 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
    25381 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
    25441 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
    25501 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
    25561 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
    25621 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
    25681 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
    25741 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
    25801 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
    25861 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
    25921 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
    25981 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
    26041 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
    26101 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
    26161 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
    26221 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
    26281 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
    26341 aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc
    26401 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
    26461 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
    26521 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
    26581 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
    26641 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
    26701 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
    26761 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
    26821 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
    26881 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
    26941 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
    27001 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
    27061 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
    27121 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
    27181 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
    27241 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
    27301 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
    27361 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
    27421 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
    27481 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
    27541 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
    27601 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
    27661 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat
    27721 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
    27781 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
    27841 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
    27901 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
    27961 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
    28021 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
    28081 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
    28141 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
    28201 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
    28261 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
    28321 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
    28381 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
    28441 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
    28501 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
    28561 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
    28621 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
    28681 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
    28741 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
    28801 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
    28861 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
    28921 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
    28981 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
    29041 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
    29101 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
    29161 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
    29221 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
    29281 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
    29341 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
    29401 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
    29461 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
    29521 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
    29581 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
    29641 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
    29701 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
    29761 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
    29821 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
    29881 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
    29941 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
    30001 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
    30061 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
    30121 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
    30181 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
    30241 tgaataattt cttaaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
    30301 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
    30361 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
    30421 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
    30481 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
    30541 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
    30601 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
    30661 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
    30721 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
    30781 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
    30841 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
    30901 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
    30961 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
    31021 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
    31081 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
    31141 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
    31201 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
    31261 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
    31321 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
    31381 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
    31441 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
    31501 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
    31561 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
    31621 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt
    31681 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag
    31741 aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt
    31801 ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta
    31861 ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg
    31921 cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc
    31981 tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt
    32041 taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa
    32101 aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca
    32161 caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac
    32221 acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc
    32281 tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg
    32341 gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca
    32401 ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac
    32461 aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac caagatatgc
    32521 gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta
    32581 agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa
    32641 ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta
    32701 aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt
    32761 ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa
    32821 acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg
    32881 tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa
    32941 aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga
    33001 actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca
    33061 cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg
    33121 taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc
    33181 gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta
    33241 tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca
    33301 gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag
    33361 ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag
    33421 aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa
    33481 aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt
    33541 ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg
    33601 ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc
    33661 agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga
    33721 cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt
    33781 caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg
    33841 atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca
    33901 attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac
    33961 gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat
    34021 tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat
    34081 gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc
    34141 caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg
    34201 atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg
    34261 aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca
    34321 gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt
    34381 atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca
    34441 agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa
    34501 gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac
    34561 gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta
    34621 cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct
    34681 atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt
    34741 aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt
    34801 tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga
    34861 ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa
    34921 aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc
    34981 atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga
    35041 cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag
    35101 aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc
    35161 taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct
    35221 tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc
    35281 tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga
    35341 cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa
    35401 acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat
    35461 cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa
    35521 ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt
    35581 atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg
    35641 atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc
    35701 acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa
    35761 aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac
    35821 cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg
    35881 ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga
    35941 tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact
    36001 cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag
    36061 cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc
    36121 caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa
    36181 acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc
    36241 attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga
    36301 agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat
    36361 tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg
    36421 ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg
    36481 cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa
    36541 aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca
    36601 gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta
    36661 atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac
    36721 gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg
    36781 aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg
    36841 tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta
    36901 tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag
    36961 caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt
    37021 tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat
    37081 atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt
    37141 taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat
    37201 ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc
    37261 caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag
    37321 gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc
    37381 gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg
    37441 aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct tgagacactt
    37501 atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca
    37561 ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag
    37621 ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc
    37681 aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact
    37741 ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt
    37801 ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa
    37861 tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta
    37921 actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg
    37981 gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca
    38041 ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg
    38101 aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag
    38161 acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag
    38221 cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag
    38281 tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa
    38341 actctgatga agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat
    38401 taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa
    38461 aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc
    38521 gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg
    38581 gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat
    38641 ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa
    38701 acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa
    38761 acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg
    38821 tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc
    38881 taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt
    38941 aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa
    39001 aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt
    39061 gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta
    39121 ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt
    39181 cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca
    39241 gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag
    39301 gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat
    39361 tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac
    39421 cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca
    39481 aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata
    39541 aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta
    39601 aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag
    39661 tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt
    39721 tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca
    39781 gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact
    39841 gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt
    39901 gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt
    39961 ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa
    40021 aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa
    40081 gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat
    40141 tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc
    40201 gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg
    40261 gtgacaaaaa cggacacgta gggtttggta caggtaaagc tcaagaagtt cctgaagcaa
    40321 tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa
    40381 caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg
    40441 tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg
    40501 tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa
    40561 cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat
    40621 cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt
    40681 aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct
    40741 tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa
    40801 tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac
    40861 attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc
    40921 aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg
    40981 gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac
    41041 aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact
    41101 atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg
    41161 ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta
    41221 atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag
    41281 aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga
    41341 tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt
    41401 atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct
    41461 ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt
    41521 ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct
    41581 tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa
    41641 aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg
    41701 tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc
    41761 aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg
    41821 agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat
    41881 catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga
    41941 tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt
    42001 gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac
    42061 gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg
    42121 agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc
    42181 attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta
    42241 taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta
    42301 cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta
    42361 tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg
    42421 tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca
    42481 aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat
    42541 cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt
    42601 tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt
    42661 tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa
    42721 cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt
    42781 tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata
    42841 aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac
    42901 cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag
    42961 ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg
    43021 ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag
    43081 caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg
    43141 ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta
    43201 tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca
    43261 ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa
    43321 aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa
    43381 attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga
    43441 attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac
    43501 aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa
    43561 actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact
    43621 gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt
    43681 gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac
    43741 tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg
    43801 aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa
    43861 aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt
    43921 agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa
    43981 cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga
    44041 attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg
    44101 ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg
    44161 tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg
    44221 tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg
    44281 aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat
    44341 agaagcaggt attgcacata tccattctac attcaataac actatcatca tgatcactga
    44401 tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa
    44461 aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga
    44521 acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc
    44581 aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt
    44641 tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt
    44701 gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa
    44761 gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca
    44821 agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc
    44881 gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac
    44941 tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca
    45001 ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta
    45061 ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta
    45121 acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag
    45181 tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg
    45241 gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga
    45301 acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg
    45361 gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg
    45421 atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag
    45481 aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct
    45541 cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg
    45601 agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa
    45661 aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg
    45721 aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc
    45781 gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga
    45841 aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg
    45901 cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg
    45961 aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg
    46021 cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg
    46081 caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga
    46141 ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc
    46201 tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa
    46261 ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt
    46321 gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc
    46381 atggagccaa ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc
    46441 cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga
    46501 cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa
    46561 tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga
    46621 cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa
    46681 gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt
    46741 gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt
    46801 ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat
    46861 atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc
    46921 tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat
    46981 attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt
    47041 gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat
    47101 ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt
    47161 gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag
    47221 acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga
    47281 cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct
    47341 tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac
    47401 gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca
    47461 tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt
    47521 tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat
    47581 tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag
    47641 ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat
    47701 tggatgaacc aacggcagga cttgatcccc agggtagaaa agaaatgatg gagatgtttt
    47761 ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg
    47821 tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg
    47881 agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta
    47941 ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt
    48001 tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg
    48061 aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc
    48121 cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc aaataattgg
    48181 gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat
    48241 ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct
    48301 ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc
    48361 actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt
    48421 atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac
    48481 ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg
    48541 attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag
    48601 cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt
    48661 cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct
    48721 atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg
    48781 cataatagag acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt
    48841 ttgagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa
    48901 gaaaggatgc gagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca
    48961 ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttataaaaaa atcattattc
    49021 cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc
    49081 atacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga
    49141 agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc
    49201 gggaaggaca gatgccggag tacatgcgat tgggcaagta atccattttg attatccaga
    49261 ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc
    49321 tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa
    49381 gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc
    49441 aagttatttt ccttaccctc ttgatttgga aaaaattcag cgggcgcttc ctgatttatt
    49501 agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaagtacg
    49561 tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt
    49621 taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat
    49681 tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa
    49741 tgcagcagga cctacagcac atcctgaagg actttatttg tatgaagtga aatatgaata
    49801 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattactt
    49861 ctcaaaattt tgcaaaattt cggcttattt tcgaaggagc tacttctgga acaccgtgaa
    49921 tcagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccaaa caaaaatagc
    49981 tcgtcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    50041 taaggaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggaaaaa
    50101 tagttcctca tattttgccg tttgttgagc taataccgaa ggtcagctga ttgaacaccg
    50161 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaagtaac aatttcaaaa
    50221 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    50281 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    50341 cttaatgagg acagtcggaa caggtttgtc atttttatat ttaaatggca caaatttttc
    50401 taattatttg tcatttagcg ctgaaaattt ttgctattta tctaaaaaag tgataactac
    50461 ttaagatgaa gcagaatatt tacgccatac ggcatacaac cgcgaaaaat ataaaaaaaa
    50521 gaaaacgatt gaacgcgttt ttgcagtcgc aaaggaaaag catggaatgc gctacatcaa
    50581 gtatcgagac ttggaaaaca atcaggtgta tacgctgatt gtttttgctg caatgaatct
    50641 gaaaaagttg tccacttggc agttggaaca tatgtcttca tatcttttat tatacaaaaa
    50701 tagcaaaagc accagtctac actcttagta aagaggaaac tgatcctttt gtctacattc
    50761 ttaaagtact taaactaagt actttatttt actctttagg attgatacag tagcatctat
    50821 ccctttatta ataatctttt atttttttaa ataaagatgt tgtctccatc ttcaggcact
    50881 aaaacttttt cggaaattcc tttttcttta ttgaaagact ttaattcttc tcttgacagt
    50941 ccccaatggt ttacagcttc catatgaacg gcaataattg tggaatctgg tattgcttta
    51001 tgcaattcat ataaatcgtc tttccccata ataagagatt ctccaactaa aaaatgatta
    51061 tctccagcat ttgctactat gatttctgga ttatactgat caatagtttg tttaacagct
    51121 tcataccaaa ctgtatctcc tgtaaagtat agtgtcttct cagtcgaatg tttgaaaaca
    51181 ataccgcaaa cattacctac ataattcaag atttctcctc tgccatgttt gccttttgtt
    51241 ttaaccaatt gtatagattc aaaaactgtg tcttctcgta gtacctccac atttttaaaa
    51301 ccatatagtc gaatttcatt cgcatcattt tcatcttgca caaataattt tatttcttta
    51361 ggtaggacct ctttcgctat atcgtcccaa tgatctagat gtaaatgtgt tacaataaca
    51421 gcatcaatac cgttgataat atcttcgact ggcattggca aatcaactag aggattcatt
    51481 tgatcttttc ttggtgatgg aaaaggacct ttcgaaataa atggtggaag tgttcctttt
    51541 tccgctaaca ttggatcaat taaaaatttt ttacctccat agtctaagat agctgttgca
    51601 ttacgaatgt gatgtatatt cataaacgtt gtctcctttt tgttgatatg aaatattttt
    51661 agtataattc aattccatac aataaataaa ccgaacaaaa aaagtctttt actattttta
    51721 cttaatttat gaaaggttgt ttaccatgtt agaccaaaca gatataaata tcattgagga
    51781 actatcaaaa aatagtcgaa tcacaatgaa agaacttgga gaaaaagtac atttgactgg
    51841 acccgctgtc tcggcgagag tgacaaaact agaggaaagt ggagttattg aaaattatac
    51901 agt
//
LOCUS       DACDOA010000010        44859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDOA010000010 DACDOA010000000
VERSION     DACDOA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.010"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWI39_000500"
     CDS             212..604
                     /locus_tag="IWI39_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9207629.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWI39_000501"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWI39_000501"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9207630.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWI39_000502"
     CDS             3165..4775
                     /locus_tag="IWI39_000502"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9207631.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWI39_000503"
     CDS             5067..5936
                     /locus_tag="IWI39_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9207632.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWI39_000504"
     CDS             6101..7372
                     /locus_tag="IWI39_000504"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9207633.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWI39_000505"
     CDS             7369..8664
                     /locus_tag="IWI39_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9207634.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWI39_000506"
     CDS             8763..9026
                     /locus_tag="IWI39_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9207635.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWI39_000507"
     CDS             9471..9920
                     /locus_tag="IWI39_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9207636.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWI39_000508"
     CDS             9977..11395
                     /locus_tag="IWI39_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207637.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWI39_000509"
     CDS             11395..12255
                     /locus_tag="IWI39_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9207638.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWI39_000510"
     CDS             12429..13202
                     /locus_tag="IWI39_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9207639.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWI39_000511"
     CDS             13349..13714
                     /locus_tag="IWI39_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9207640.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWI39_000512"
     CDS             complement(13862..15070)
                     /locus_tag="IWI39_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9207641.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWI39_000513"
     CDS             complement(15089..15367)
                     /locus_tag="IWI39_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207642.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWI39_000514"
     CDS             15560..16216
                     /locus_tag="IWI39_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9207643.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWI39_000515"
     CDS             complement(16658..17455)
                     /locus_tag="IWI39_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9207644.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWI39_000516"
     CDS             complement(17452..18636)
                     /locus_tag="IWI39_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9207645.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWI39_000517"
     CDS             complement(18661..19908)
                     /locus_tag="IWI39_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9207646.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWI39_000518"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWI39_000518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9207647.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWI39_000519"
     CDS             21888..22202
                     /locus_tag="IWI39_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9207648.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWI39_000520"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWI39_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9207649.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWI39_000521"
     CDS             22887..23531
                     /locus_tag="IWI39_000521"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9207650.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWI39_000522"
     CDS             23546..23875
                     /locus_tag="IWI39_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207651.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWI39_000523"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWI39_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9207652.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWI39_000524"
     CDS             24863..25687
                     /locus_tag="IWI39_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9207653.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWI39_000525"
     CDS             25680..26027
                     /locus_tag="IWI39_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9207654.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWI39_000526"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWI39_000526"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9207655.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWI39_000527"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWI39_000527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9207656.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWI39_000528"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWI39_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9207657.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWI39_000529"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWI39_000529"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9207658.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWI39_000530"
     CDS             31684..32451
                     /locus_tag="IWI39_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207659.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWI39_000531"
     CDS             32441..34483
                     /locus_tag="IWI39_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207660.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWI39_000532"
     CDS             34764..34913
                     /locus_tag="IWI39_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9207661.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWI39_000533"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWI39_000533"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9207662.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWI39_000534"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWI39_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9207663.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWI39_000535"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWI39_000535"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9207664.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWI39_000536"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWI39_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9207665.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWI39_000537"
     CDS             39330..40412
                     /locus_tag="IWI39_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9207666.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWI39_000538"
     CDS             40412..41215
                     /locus_tag="IWI39_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9207667.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWI39_000539"
     CDS             41285..42025
                     /locus_tag="IWI39_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9207668.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWI39_000540"
     CDS             42036..43040
                     /locus_tag="IWI39_000540"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9207669.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44848)
                     /locus_tag="IWI39_000541"
     CDS             complement(43085..44848)
                     /locus_tag="IWI39_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207670.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14421 a         7826 c         9341 g        13271 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataa
//
LOCUS       DACDOA010000011        43605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDOA010000011 DACDOA010000000
VERSION     DACDOA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.011"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(57..932)
                     /locus_tag="IWI39_000542"
     CDS             complement(57..932)
                     /locus_tag="IWI39_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9207671.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1080..1412
                     /locus_tag="IWI39_000543"
     CDS             1080..1412
                     /locus_tag="IWI39_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9207672.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1524..2732
                     /locus_tag="IWI39_000544"
     CDS             1524..2732
                     /locus_tag="IWI39_000544"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9207673.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2886..3341
                     /locus_tag="IWI39_000545"
     CDS             2886..3341
                     /locus_tag="IWI39_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207674.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3703..3978
                     /locus_tag="IWI39_000546"
     CDS             3703..3978
                     /locus_tag="IWI39_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207675.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3991..6069
                     /locus_tag="IWI39_000547"
     CDS             3991..6069
                     /locus_tag="IWI39_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9207676.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6229..8115
                     /locus_tag="IWI39_000548"
     CDS             6229..8115
                     /locus_tag="IWI39_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9207677.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8127..9074
                     /locus_tag="IWI39_000549"
     CDS             8127..9074
                     /locus_tag="IWI39_000549"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9207678.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9094..9621
                     /locus_tag="IWI39_000550"
     CDS             9094..9621
                     /locus_tag="IWI39_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9207679.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9684..10337)
                     /locus_tag="IWI39_000551"
     CDS             complement(9684..10337)
                     /locus_tag="IWI39_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9207680.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10469..11311
                     /locus_tag="IWI39_000552"
     CDS             10469..11311
                     /locus_tag="IWI39_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9207681.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11469..12365
                     /locus_tag="IWI39_000553"
     CDS             11469..12365
                     /locus_tag="IWI39_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9207682.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12368..13081
                     /locus_tag="IWI39_000554"
     CDS             12368..13081
                     /locus_tag="IWI39_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9207683.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13101..13619
                     /gene="msrA"
                     /locus_tag="IWI39_000555"
     CDS             13101..13619
                     /gene="msrA"
                     /locus_tag="IWI39_000555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9207684.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13616..13843
                     /locus_tag="IWI39_000556"
     CDS             13616..13843
                     /locus_tag="IWI39_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9207685.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13995..14546
                     /gene="lepB"
                     /locus_tag="IWI39_000557"
     CDS             13995..14546
                     /gene="lepB"
                     /locus_tag="IWI39_000557"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9207686.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14629..15171)
                     /locus_tag="IWI39_000558"
     CDS             complement(14629..15171)
                     /locus_tag="IWI39_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9207687.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVFFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15408..15806)
                     /locus_tag="IWI39_000559"
     CDS             complement(15408..15806)
                     /locus_tag="IWI39_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9207688.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16066..16926
                     /gene="ylqF"
                     /locus_tag="IWI39_000560"
     CDS             16066..16926
                     /gene="ylqF"
                     /locus_tag="IWI39_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9207689.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16919..17686
                     /locus_tag="IWI39_000561"
     CDS             16919..17686
                     /locus_tag="IWI39_000561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9207690.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17742..18599
                     /gene="dprA"
                     /locus_tag="IWI39_000562"
     CDS             17742..18599
                     /gene="dprA"
                     /locus_tag="IWI39_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9207691.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18721..20799
                     /gene="topA"
                     /locus_tag="IWI39_000563"
     CDS             18721..20799
                     /gene="topA"
                     /locus_tag="IWI39_000563"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9207692.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20768..22144
                     /gene="trmFO"
                     /locus_tag="IWI39_000564"
     CDS             20768..22144
                     /gene="trmFO"
                     /locus_tag="IWI39_000564"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9207693.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22342..23247
                     /gene="xerC"
                     /locus_tag="IWI39_000565"
     CDS             22342..23247
                     /gene="xerC"
                     /locus_tag="IWI39_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9207694.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23284..23832
                     /gene="hslV"
                     /locus_tag="IWI39_000566"
     CDS             23284..23832
                     /gene="hslV"
                     /locus_tag="IWI39_000566"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9207695.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23846..25245
                     /gene="hslU"
                     /locus_tag="IWI39_000567"
                     /pseudo
     CDS             23846..25245
                     /gene="hslU"
                     /locus_tag="IWI39_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
     gene            25263..26054
                     /gene="codY"
                     /locus_tag="IWI39_000568"
     CDS             25263..26054
                     /gene="codY"
                     /locus_tag="IWI39_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9207696.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26136..27011
                     /locus_tag="IWI39_000569"
     CDS             26136..27011
                     /locus_tag="IWI39_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9207697.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27143..27781)
                     /gene="plsY"
                     /locus_tag="IWI39_000570"
     CDS             complement(27143..27781)
                     /gene="plsY"
                     /locus_tag="IWI39_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9207698.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27908..28342
                     /locus_tag="IWI39_000571"
     CDS             27908..28342
                     /locus_tag="IWI39_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9207699.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28508..30553
                     /gene="parE"
                     /locus_tag="IWI39_000572"
     CDS             28508..30553
                     /gene="parE"
                     /locus_tag="IWI39_000572"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9207700.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30565..33015
                     /gene="parC"
                     /locus_tag="IWI39_000573"
     CDS             30565..33015
                     /gene="parC"
                     /locus_tag="IWI39_000573"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9207701.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33292..35523
                     /gene="pflB"
                     /locus_tag="IWI39_000574"
     CDS             33292..35523
                     /gene="pflB"
                     /locus_tag="IWI39_000574"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9207702.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35767..36528
                     /gene="pflA"
                     /locus_tag="IWI39_000575"
     CDS             35767..36528
                     /gene="pflA"
                     /locus_tag="IWI39_000575"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9207703.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36613..37539
                     /locus_tag="IWI39_000576"
     CDS             36613..37539
                     /locus_tag="IWI39_000576"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9207704.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37721..38614
                     /locus_tag="IWI39_000577"
     CDS             37721..38614
                     /locus_tag="IWI39_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9207705.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38748..38873
                     /locus_tag="IWI39_000578"
     CDS             38748..38873
                     /locus_tag="IWI39_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207706.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(39007..39639)
                     /locus_tag="IWI39_000579"
     CDS             complement(39007..39639)
                     /locus_tag="IWI39_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207707.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(39787..40329)
                     /locus_tag="IWI39_000580"
     CDS             complement(39787..40329)
                     /locus_tag="IWI39_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9207708.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40464..40853
                     /locus_tag="IWI39_000581"
     CDS             40464..40853
                     /locus_tag="IWI39_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207709.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            40857..41621
                     /locus_tag="IWI39_000582"
     CDS             40857..41621
                     /locus_tag="IWI39_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9207710.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            41750..42697
                     /locus_tag="IWI39_000583"
     CDS             41750..42697
                     /locus_tag="IWI39_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207711.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            42827..43567
                     /locus_tag="IWI39_000584"
     CDS             42827..43567
                     /locus_tag="IWI39_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207712.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT        15188 a         7486 c         9135 g        11796 t
ORIGIN      
        1 gacaaaggtc agctggatgt ggaatctttc ctcatagcct atctttgtct tttttcttat
       61 agttttttca gtaatcttgt ctgtggcttc aaataagtaa agccttgccc ttccacttca
      121 tgcacattga tgattgaaag gaaagctttt tcgtcaatct cgtggacgat cctcttagct
      181 tcagttaatt cactagagct aacgaccata tagatgattt tttttgaatt accagagaat
      241 gcgccttctc cagaaaggaa agtcacacct ctttgcagac cggtcatcag tagtggtgca
      301 atctcttcgc ttttgttgga gacgacaagt agaccttttg cagaatatcc gccatctaag
      361 atcgaatcaa tcacacgact gaaaacatac gaaacaatca acgtatacat catacgtttc
      421 aagtcgatat aggttaatga agcaagtaag acgatgatat caaatgccaa tagggaacgc
      481 cccatagtga tgccgtaatt ttgttctaaa atccgagcga taatatcact gccgcctgtt
      541 gttccaccca tgcggtagac gatcccgctt cctacacctg caactaaacc cgcaagtaga
      601 gaaacgatca gtagatcatg ctgtaaattg atttctagtg gaaccctttg ccagatccat
      661 aaaaagaatg acaaagaaag tgtacctaga acagtatagg ccaaggaacg tttaccgagg
      721 atctttcctc ctagtaaaat caatgggatg ttgattaata aagtcgtata agctggatca
      781 ataccaaata aagctcttaa gatcaaggtg atccctgaga ctcccccttc tgctaaatga
      841 ttagcgatgt tcaagtaaac aagaccaaat gcataaatac ttgttcctat aatgataaaa
      901 agaacttctt ttactgtttt tcgtttgacc atatgaattc cagctttcaa ttatgtattt
      961 tcacagaatt tccatttaga aattccttcc taaatgtagc atggaaattc gattttctca
     1021 attgctctta tctaggattt gcgttaaagt tttggtaaga tagttttgga gggaaaaaga
     1081 tggataaaaa gcgttcttta tacagtatgc agcaggaagt ggatgaatat atccagcaat
     1141 tcaaggtggg gtatttctct ccattagctc agatggctcg attaactgaa gaagtaggtg
     1201 aattggcaag agaagtcaac cacacttatg gagaaaaaag caaaaaggct tctgaaccag
     1261 ttaactcggt agcagaagaa cttggtgatg ttttattcgt tacgatgatc atggctaatt
     1321 cattgaatat cgacttaact gaagtttttg aaaaaaacat ggaaaaattc aatcaaagag
     1381 accataatcg ctttgagcgt aaggacgagt aaacaagatg aagtggtcct ggccttgaag
     1441 actctgaaac ggacttgtca aacagtcacc atgcaactga gtctttatct caggacaaga
     1501 aacacgcagg aaaggttgga aaaatgaaat taaaacaatt acctctggaa taccaaaagg
     1561 caattccagt tctgaaaaaa ttagagaatg caggatatga agcttatttt gtaggtggaa
     1621 gtgttcgtga tatcttgctg aaccagccaa tccatgatgt ggatatcgca acaagcgcat
     1681 ttccagaaga aatcaaacag ctttttccaa aaacgataga tattgggatc gaacatggaa
     1741 ctgtgttggt attagaaaat gatgaacaat atgagatcac cacatttcgg acggaatcaa
     1801 cttatcagga ctaccgtaga ccagatcatg tcgaattcgt tcgttcgttg gcagaagatt
     1861 tgaaaagaag agattttacg atcaatgcgt ttgctttgaa agaagatgga gaaatcatcg
     1921 atttgttcga tggtctgcaa gatctagaag aacagatctt gcgagctgta ggcaatcctc
     1981 atgagcggtt ccatgaagat gccttacgaa tgatgcgcgg gttgcgtttt gtcagtcaac
     2041 tagggttcaa attagaggaa gaaaccttct acgccatcaa agaaaaccac gagctattag
     2101 gtaaaatatc tgtagaacgt ataaatgtgg aattcatcaa gatgctcttg ggaaatgaac
     2161 gggccgcggg aatccatgca tttattgaaa cagagtgcta catttgctgt cccggtttga
     2221 aagaagaagg cgaagcactt ttacgttttg ctgaattacc atccgtccca ttgaaagaag
     2281 agagccaagc atgggcatta ttgatcagcc aactggatct aaaagaaaat gagattcgtt
     2341 cctttttgaa atcgtggaag tgttctaacc aaatgattaa agacgttcaa gcacttgtcc
     2401 aaggactacg acaacgagaa actggagctt tggaaccatt cgaattgtac ttcttaggca
     2461 aaaaacatgc actgcaagta gaagaattga tgatctattt tgacgaaaca cctcagatgg
     2521 atgccgtagg agaatcctat gatcgtttac ctatcaagtc attaagcgaa ttagcagtag
     2581 atggtaaaat cttgctagaa gaaactggaa aaaaacctgg taagtgggtt agtgaagctt
     2641 tgcaattagc tgaaaaagcc gtcgtagaac aaagaataaa aaatgataaa aaacaggttt
     2701 tggatttttt atcgaaagaa aaattgattt aaaaaacact agactgtatc tgctcagctt
     2761 tgttggctac ttaaagtggg cagatacgtc attagaagaa aaaacataag atgtgaccac
     2821 ttacataaat ttgtgctaaa ctgaggtagt acaagaataa attacatcat aaaggagtct
     2881 ttttaatgag taaagaaatg acagcgctaa aattttattt tagaaatgga gaaacctgga
     2941 cgatcaaccg ccgtcatatc ggcgatcttt ggatcaaaca aatcacaaca agttttggac
     3001 gaatcaatgg cagtgaattt gttgaaatcc atccgtgtgc tggatttaaa atcgaaatct
     3061 tccatgaagg ggacgcagtt gcgacacatg atatcaacct aggcggactg gaaatgggca
     3121 tgtttaatcg cgcattgaaa tacgaagata tcgagcgaat ggaaatcctt taccgaaatg
     3181 gcacacctga tcttgtgtac ttcccatatc tagataaagg aacagaagga ttagacaatc
     3241 aatatcaatc aacaaaaatc agcgaaaaaa caggtaactt gtatatcgtg atcaatccag
     3301 atcaacgtgt agaagatgta tacggggaat ttttcgaata aatcgatttt tacgcaaaaa
     3361 tgaggctgtg aaagagatgt gtcttattac tactgatagg ctgtctcttc cacaccgttt
     3421 attcagaata agggactgtg acaagatttt tgtcgcagcc ccattttttt gttgtgataa
     3481 aagaacaaga aaggcaggaa tgggaaagta aacatccttt tgtcagaaac ttttatcttt
     3541 cttaagtttt acttgatatc tgatactatt tggaatagaa tgaggcgttg aaaaatttat
     3601 ggaaatttct aactagcaat aaacgatgaa acaatgaaat atttttcatc caaaaagaac
     3661 tttgctataa tgatggaagt ttgtgtatag gagatctgga aaatgagaga gagttttgaa
     3721 caacaaaaaa aactgttgca tgatcgctat ggcgcccttt cgatggatga ccgccgtcaa
     3781 atactttgta agttgcgtaa aagaaatata ttgatgtatc gtcagttgga acgtctgaaa
     3841 catgatctgc ttcgtttgga gtcaaaacgt gtacagtgtg aattagaggg gaaccaaaca
     3901 caggttgaag tagtcgaaac caaaatttta aagaaaaaag aacaattttt aaaaatgctt
     3961 acacaaaata aaaaataaag gggatggacg atggaatttg ctgagttagt tattgtattt
     4021 gcagtaacga tcaccttatc gaacatagct agccgcattt tgccaatgat tccagctcca
     4081 ctgatccaaa ttttccttgg agtggtatta ggtctgacag agtggggaca atcaatcgat
     4141 tttgaacctg aattgttctt agttatgatt attgcaccat tgttatttag agaaggagaa
     4201 aaagcagata tttcttcgat tctcaaaaat ttcgggacaa ttttgttctt ggcatttgga
     4261 ggagtcattt tgacccttgt cggggtaggt gcgacgttat cattcctgtt gccaagtgtc
     4321 ccacttgctg cctgctttgc ttttggagca gcgttaggtc cgacagatgc ggtagcagtc
     4381 agttctcttt ctggacgagt gaatatccct aaaaaggcga tgcatatttt ggaaggagaa
     4441 ggtcttttga atgatgcatc tggtgttact gccttccagt ttgctcttgg tgcgctgatc
     4501 accggatctt tttcagcagt aaacgcgggg atgagtctag tagtttctag tattggggga
     4561 gctttgatcg gctttttgct tgtctggttc aaacaaaaaa taattcattt gatcgaaaaa
     4621 gcatcagcac aagatgtcac agggtatctg ctgatcgaac ttttgcttcc ttttctagcc
     4681 tatgtattgg cagaggcatt tgatgtatct ggaatcattg ctgcagtggc agcaggtatt
     4741 ctccaagctt ctggttttag gaaaatctct gtgtttgatg cagaattgtc gagcttgtcc
     4801 cacagtacat ggacaactat tgcttttaca ctgaatgcat tagtatttat atttttagga
     4861 atcgaattga cgcaagtgtt ctcgccagta tggggagacg gtttatatcc aaacggtctt
     4921 ttattagcga ttatcgtatt aatctctgtc atgctttttg tgatcagatt catttctatc
     4981 agtctttttt atgtattcaa agatggcagt aaaaagttca aaaaacagtt gaacgagatc
     5041 ttgatcttaa catttggcgg tgtaaaggga acagtcagtt tggctacgat ttttattttg
     5101 ccattttcaa tcaataatat gatgttttac cagcggtctt tgcttttgtt tctgacggcg
     5161 ggtgtcatct tagtgacttt ggtcataggg atcattgtct tgcctatgct gacggaaaca
     5221 gaggaagcga aaagcacgga tttgaatgct ttgatgattt tagaagaggt cgttgaagtt
     5281 ttgcgaaaag aaataaagga aattgatcgt aatacaaaag aatttttagc cactgaagca
     5341 gtgatagaaa attatcaaga acggatacgt gatctgtact tggaagattt aaccgatgat
     5401 gaaaagcaag aagtacaaga aatacaagca ctgattttgt cgatcgaacg ggatggattg
     5461 gatgaaagtt atcgttcggg caagctttct tctaatggtt atcgttttta ctcccgtttt
     5521 ctttctcgat tcgaacattc tatcacaagc cagattttgt cttttatcgg cttttggttc
     5581 attgttgtcc gtaggttaat gcgaatcgtt ctccatccga aaatgttttg gcaaagaaga
     5641 tatgctgatc gtcagacatt tattagtgat gaagatatcc aagaaatcag agagacatat
     5701 caaaaaaata ctcagttgat tattgaaagt ttagataact tgactgatat ttatgatgaa
     5761 acgatggttc aattttttat ccagcaacgc aaacttgaag gaataaaaat gaactcgggg
     5821 aatttgattt cttcgtggat gatccagcaa gatagtctgt ttacgaaaaa aatgctaaga
     5881 ggttattatc ttgaaagaaa aatcattgat gaatacgaag ttgctgaaaa aatcacgacg
     5941 ttttctgcga acagctatcg caggaatatc aatttgttag aatcttatac gatgaacaaa
     6001 ccatctgata acttttcttt ccgatttgcg tttcgagcaa agtcaaaaaa aacgagtcca
     6061 ataaaataaa aaggtaaaaa ttttcaagtt ttataaaaaa caaaacatat cagaacagga
     6121 tcgaatcggt cctgttcttt ttttgagaac acaaaaagaa caaataccaa aaaaattttc
     6181 ctgaagttca ctatttttat gatacaatat ccaagataag gagggaacat gttgaaagaa
     6241 ttaaaagtaa ctgacctcaa aaaaacttat ggtgaaaaag atttgtttga ccagatttct
     6301 tttttggtcc acgaaaaaga ccgtatcggg ttgattggga cgaacgggac gggtaaaaca
     6361 agtctcttaa atattattgc cggtatcgat agcagcgacg gtgatcggca aacagtgttt
     6421 tatccaactg attaccgtat tggttatctt tctcaagcag ctgaattttc cgatgaatta
     6481 actgttctcc aagcagtctt tcaaggaaac agccccttga tccaaactgt tcgagcatat
     6541 gaggaagcat tgatcgaact aggggaaaac ggagaagacc ctgatgtgca aaaacgttat
     6601 gctaaagcag aagaacaaat gaataaagaa gatgcttgga ctacagatac caacgcaaag
     6661 attattttgc aaaaattagg aatcagtgaa ttagataaaa aaatcagtac tttatcaggt
     6721 ggtcagaaaa aacgagttag tctggcgcaa gtgttgattg atgaacccga tttactatta
     6781 ctagacgaac caacaaacca tttagattat gaagcgatcg agtggttgga aaactatttg
     6841 aaacaatacc gtggcgcact attgatggtt acccacgatc gttattttct agatagagta
     6901 gcaaaccgaa tcttcgaact ttcatttgga aaattatacg agtataaagg aaattatgaa
     6961 gcttacgtct tagaaaaagc agaacgtgat cgtgtagaag tagaacaaga agaaaagcgc
     7021 aagcggcttt ataaacagga attagcttgg atgagggctg gcgtacaggc acgtggcacc
     7081 aagcagcaag cacggatcaa ccgtttcgaa gatctaaagg agaacttgta ccaagtaaac
     7141 caagaagatg atttagagct gaacctagcg acacaacgtt tagggaaaaa agtactggaa
     7201 atcaaagatg gcagttaccg tataaatgac caaacactgc tagaacattt agatttattg
     7261 attcaatctc gggaaagact aggtattact ggtaaaaatg gtgcgggaaa atcgacattg
     7321 cttaatattc tagctggcag aattcccctg gatagcggta caatgtctat cggtgaaacg
     7381 gttcatcttg cgtattatac acaagaaaat gaagaaatgg caccagataa acggatgatt
     7441 gcttacttac aagaagctgc tgaagaagca aaaacagcag atggcagtca aatcggagta
     7501 gcagaattac ttgaacgatt tttatttcca agatttatgc atgggacact gataggcaaa
     7561 ctttcaggcg gagaaaaacg ccggttattt ttgttgaaac tgctgattca gcaacctaat
     7621 gttttattat tggatgaacc gaccaatgat ttggacatag caacattgac gattttggaa
     7681 gattatttta gaagttttcc aggtgctgtg atcactgttt cccatgaccg ttatttcttg
     7741 gacaaggtag cagataagtt attagtattc caaggaaacg gaaaacaaga actttattac
     7801 ggaaacatgt ccagttattt actgaagcaa aaagagaccc agcagcctgc tgaaaaagcg
     7861 aaaccaaaaa tacagagcaa ggaacctgct gaaaagaaga aattatctta catggaacaa
     7921 aaagaatggg aaacgattga agatgagatt gcagaacttg aagaaaaaat ctcattgctc
     7981 caagaagaga tgaatcatca aggagataat ttcactcgat tacaagaatt gcaaaacgac
     8041 gtatctgaaa cagaagcaca acttgaagaa aaaatggcac ggtgggaata cttgagtgaa
     8101 tgggtagaag attaggagga aaaaggatgg aacaagcgta tcttgactta ggaagaaaat
     8161 tgttggaaga agggcatcgt aaagaagaca gaacaggcac aggcacatta agcttattcg
     8221 gataccaaat gcggtttgat ctgcaaaaag ggtttccttt gttaacaacg aaacgagtac
     8281 cttttggatt gatcaaaagc gaattgcttt ggtttttaaa aggagatacg aatatccgct
     8341 atctattaca aaataaaaac catatttggg atgaatgggc atttgaacgc tacatcaaaa
     8401 gtccagacta taatggacca gatatgactg attttggcca tagatgtttg acagatcctg
     8461 aattcaatga agaatataaa aagcagagca agcttttttg cgagaagatc ctaaccgatg
     8521 attcctttgc tgaaacatac ggcgatcttg gacacatcta cggctatcaa tggcgtcact
     8581 gggaaacaaa agacggtggc tttatcgatc aaatcaaaca agtgatcgaa gcaatcaaaa
     8641 agactcctga ttctagaaga ttgatcgttt ctgcatggaa tccggaagat gttcctagca
     8701 tggctttgcc tccttgtcat acgatgtttc aattttatgt tcaagaggga cggctaagct
     8761 gccaattgta tcaacgcagt ggggatgtgt ttctaggcgt tccgttcaac attgcaagtt
     8821 atgcgttgct gactcaccta attgcacatg aaacaggttt agcggttggc gaatttgttc
     8881 acacattagg agatgctcat ctttatatca atcatatcga acaaatgaaa gaacaattga
     8941 gccgagaaat acgcgcattt ccgactttag ttttgaatca ggaaaagcag tcagtatttg
     9001 attttgagat ggaagatatc caattagaag gatatgaccc acatccggcg atcaaagctc
     9061 ctatcgctgt ttagaatcag aaaggatgag aaaatgttag ctgcaatttg ggcacaggat
     9121 gaaaacggat tgattggaaa agaagaccgt cttccttggc gtttgccaaa tgatctgaaa
     9181 ttcttcaaac aaatgacaga agcaaataca ctagtgatgg gacgaaaaac attcgaaggg
     9241 atgggtagcc gtccgttgcc gaatcgtcag acgattgttt tgacgagaga ttcttcctat
     9301 aaagcagaag gcgtgcatgt catgcatagt gtggaagacg tgcttgctct ggaaaaagaa
     9361 acagacggta tctttttcat cgctggaggg tcagctgttt atcaagaatt tctgccattt
     9421 tgtacgatat tataccgtac agtcatccat catgtattcg atggagatgc ttattttcca
     9481 cctgttgact ggtctgattg gtcactgatc aatactagtc aaggagaaat ggatgaaaaa
     9541 aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt aaaaagcggg
     9601 acaggcgaat tgacgatata aaaaagctat ggatagacta gctgttattt agagctgtcc
     9661 catccatagc tttatttttt ttattaagta aagaataaaa cagagaagta cataaaacct
     9721 gcacctaaca taacaaacaa atgccaaaca acatgcatat agcggataga attcaagcta
     9781 taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta ccatcaaaat aacaccagtc
     9841 attccaagag agtgataaag aggaacagca gccacgacac agagccaccc cataaaaata
     9901 tagatgatcg tagatacttt tgatactctc tcccgtttat gcaacgtcaa cgctttatat
     9961 ataatgccaa gaatcgcaaa caaccagatt aatacaaata aagtccaacc tagccatcct
    10021 cggatactca gcaaacaaaa aggagtataa cttcctgcaa tcaaaagaaa aatcgaacta
    10081 tgatcgaata cttggaacac cttttttgct tttgtaaaaa tcaatgaatg aaacaaagta
    10141 gacgttaaaa ataaaagaat catcatcgat ccatagatag cataagagac aatatggagg
    10201 gaggaatgca agtgggcacc ttttactaaa agaaccacta gtcccgcgat actcaatcca
    10261 gtcccaattc catgggtgac tgcattaaac acttcattta atatttcata cgttcgatta
    10321 aatttcgttt tttccatgaa aacggctcct ttactaggtg taattaatga aaaatcatta
    10381 tttgtctgtt atactaaaaa tagaaattgt cttacatgta gtttaacatg aataaggcat
    10441 cttgttaaag aaagagagag agaaaataat gacaaatgta aaaatcgtaa cagattcttc
    10501 atgtacgatg gaaaaaaatg tacgcgatgc tttaggcatt cgtgtcatgc cgctatccat
    10561 catgatcgac ggaacagtat atcccgatga tgaccaacta gacggcgaaa gatttatgga
    10621 aatgatggcg aaagcaaaaa atctgcccaa aacaagccaa ccaccaattg gagaattcat
    10681 ctcgatgtat gatgagctag gagaagacgg cagtgaagtg atttcgatcc atatgacaaa
    10741 aggcttgagt ggaacagtag aagcagcaag acaagccagt aatctaacaa aaacgaaagt
    10801 aacggttatc gacagtgata ccactgacca aggattatct tttcaggtaa ttcgagcagc
    10861 tgaattagca aaagaaggta aaacagtaga gaaaatcctt gaagaagtca aaaaagtaca
    10921 agacaataca aaattataca tcggaatatc cacactagat aacttggtaa aaggtggaag
    10981 aatcagccga acaactggtt tgctgtctag tgttttgaac atacgcgtgg tgatgaattt
    11041 cgcccattct gaattaatcc ctgtaactaa ggggcgagga aagaaaacgt tcgataaatg
    11101 gtttaataat ttgaaaaatg aactgaaaga attgccaaat gtgcgacaaa tcggtatttc
    11161 acatgcggat gcaaaagaat tggcgcaaga attcaaagag ggtttgcaag aattattccc
    11221 ggacatgcat atccctgtac tgcatacgaa tccggttatc gctacgcata ctggtaaaga
    11281 cgcgtttgca atcatgtatt atgtagatta gtcgaaaaga ggataagccc tcagcctgaa
    11341 aggcttgcag cttattctct ttttctttat aaaattagga aaataattgt tttctttttt
    11401 cataaagact acctttttga taaaattttt gataacatta gaaccaatcg aatacaagag
    11461 gtgaaaaaat gaaattaacg aaacgacatt tgatcctcgt tttgttccca ttattaattg
    11521 cgatcatcat ctatgcaggt ttagctgctt ctataccgaa agctggccca ctaatgaaac
    11581 cagtaactac tgtgagagaa gatccaaata aaaaagaaaa acttcactac atagctatcg
    11641 gagattcgct gactgaagga gtaggagatc aaaccaagca aggaggattc gttccccttg
    11701 tcgcaaatga tctaaaagac cgttataatt tgaccgcggt tgaaacagaa aactatggtg
    11761 taaatggtga acgaagtgac cagatcttaa agcgagtaaa aacaaaagaa gaaattcgaa
    11821 acaatatcaa aacagctgat tttatcacac tgactgttgg cggtaatgat ttgatgaaag
    11881 ttatccaaaa taatttattc gggttaacgg tcaattcctt taaaaagcca ctgaaaaact
    11941 atcaggaaaa tgtcacagaa ctattggaag aatttcgatc cctcaataaa accgcaccta
    12001 tttacatttt aggcatctac aatccatttt atttgaactt tcctgagatc actgacatgc
    12061 agacaattgt taacaattgg aacaaaggaa cagaaacggt gacaaaaaaa atgccgaact
    12121 gctactttat cccaatcaat gatttgcttt atcaaggatt gaatcaggaa gtaggtgtca
    12181 cagaagaagg aacagatcaa acaactagca cgacagaaaa tctaaatggc atcaaaaata
    12241 atgtgctcta tgaagaagat catttccatc caaataattt aggttatcaa ttgatggcaa
    12301 atgcagtgag agatcagctg atcgagacac aaaaccaatg gttagagaag gaggatgatc
    12361 aataaacatg aaaacaagat ctgacgtatc ttcaaacaaa acaaagaaaa caaattactg
    12421 gaaattcgcc ttccttatcc ttcttggggt agtacttggt tcgaccgtat ttttaggcag
    12481 ccgtattttt gctaatcggg aaccagaact cccagaaata ccagctctta cggaaagaca
    12541 aggagatcct gtcttaacga tcaattcaaa taaagaaaaa gtcaatcaaa tcatcagctt
    12601 tttccttagt gagtatcaaa aagatagtga tattgaatat aaattttatt tggaaaatga
    12661 agcactactc aatggtactt tcgaagtact aggttttcct atcgatttct atttatattt
    12721 tgatccgtat gtgatggaaa acggaaatgt ccaactaaaa gctagaagtc tttctatcgg
    12781 aacattgagt ttgccaatca aagatgtgat gaatatgatc aagagaaact ataaattacc
    12841 agattggatc gaagtcaata cagatgatct gactatcatg cttaggttgg atcaattccg
    12901 aatgcaaaac gggatgtata ttaaagcaga taaaatcgac ttagtaaatg atgacattcg
    12961 gtttagtcta tatttgccaa aagatacctc tgatgcttca aacaactcaa ataccaacaa
    13021 aactagcacc agcagtactg aaagcaagac aaaagaaaat aaaaaatcat cggatgagta
    13081 gtattagagg gggaatcata atggaacgag caatatttgc aggaggctgc ttctggtgta
    13141 tgatccagcc tttcgacacg cttcctggta tccatacagt gatgtcagga tatactggag
    13201 ggcatgtacc agatccgact tatgaacaag taaaaagcca aacgactgga catacagaag
    13261 cagtggagat tatctatgat ccaacattga tttcatatga acaattagta gaactttact
    13321 ggcagcaaac tgatccaact gacgctttcg gacaattcga ggatcgcggt gacaattatc
    13381 gcccggtgat cttttatttg acagaagaac agcggatcat tgctgaaaaa agtaagaaac
    13441 agttagctga aagtggacgt ttcgaacagc caatcgttac aaaaatcgaa ccagcccaac
    13501 cattttatct agcagaagaa gaccatcaag agtattataa aaagcatcct gaaaatttta
    13561 aaagaaacca tgcacgccgg gctgccttta tcgaagaaaa ttggaggaac gaagggtgaa
    13621 tcgaagcttt tttcattatt taatgacact gcgaggcggt ggcacacatg atgctttatc
    13681 tgttttcgct acagaagccg gtaaagacat ccaatttcct aagcacagct ctagctacga
    13741 agaaatttct aattatctgg aactgaatgt cgactatttg ccgtccatgg atatctttga
    13801 cgaagcatgg gaaaaatatt tagaaaataa tcatgacata taagagatag ttttgtactt
    13861 tatgatcaaa aaagtttttt gtccaatagc ctaattgaaa gaattatttt tatttcagct
    13921 atctagtcgc atttttttga tatttttctt ataataaaga aagaatgaaa aaaagaggga
    13981 tggattgata gagattgaca aaaaaacaac gctatattaa tcgcttctgg cttgtattca
    14041 aatacttact tgtttctgta ttccttgcgt tcatgttgcg gggattttta ttcatcccgg
    14101 taccagtgga aggcaattca atggaaaacg tgttgaagca aggtgatatg gttgtgatgg
    14161 aaaaattttc ggagatcaga cgatttgata ttgttgtttt tcaattagca gatggcacga
    14221 tttatatcaa acgagtaatc ggtcttccag gagaaaatgt cagctaccaa aatgatcaat
    14281 tgaagatcaa tgggaaagta gttaaggaac cttatctaac taaaaacctg aaatcagatc
    14341 atgcaaatgc atcctatacg acggacttta cactacaaga gctaacggga caatccaagc
    14401 ttccggaaga taattatttt gtacttggag ataatcgaag agtttcaaaa gatagtcgtt
    14461 cgtttggcac gatcaataaa actgatattc taggtaaagc aagatttgtc tattatccac
    14521 ttgatgaaat aaaatggatc cagtgagaaa caataaagcg tgcagtctgt tgagactgca
    14581 cgctttattg tttaatccaa aatcagttta ctaccttagt acgtaaaatc aaacagctcg
    14641 tttaaacact tttctagcct ctaaagtttt gataaatgaa taaacaacca ttatttgaat
    14701 agggatttgg atcatattcc tgatgaatct acctagaaaa aatactttga aatctgcacc
    14761 aaacaaaaca gacatcagcc aagaattgat aaaaaggctg actgggatca taatcaataa
    14821 ttgggcagcg atgacggaag gcaggtttat ctttttatga tataaaaata agccattaat
    14881 aaatccacca aggaaagccg tgataagaaa agcagcttga aacgttcctg aaccaaatat
    14941 cgtgaaattt aaagtgttcg acaacatttc aagcaaacca gcccagatcg gtccatatag
    15001 ataacccata agcgccatac ttaaaaatcc gaatgtgatt tgaaaataag caggaccaat
    15061 cgctatcttg tataagatga catcaagggc taataaaaga gcagaggtcg acaacaatct
    15121 tgcattcatt tcgggcaatc taaaaacgtc ttttgcaatc aaagtgttca aaatattctc
    15181 tcctttgtca gttaataata aaaggatacc ataaaatcac caatgtgtca tgacacattg
    15241 gtgatttaca tacactggca tctaacaatt aaaaaaaggg tgagaagact gcttttataa
    15301 actgtaaaaa agagattgta cgaataaaaa aaatagaggc ggagaatact tatcgtatca
    15361 tccgcctttg tttttttgtt ataaaaaacg tcagattagt tatctgctta catcttcatc
    15421 catacgggtt ctaatgtatt tccgtctaga tcttggactt ctaatgaaaa catttcttct
    15481 tcaggcattc ccatatctac ttcgtggaaa gtaccaccat tttcttttgc aatttgagca
    15541 aattttttta cttcatccgc attttcaaca ctaaacgaaa tcaaagcacc acttgttttt
    15601 ttattatcag ctatttcttt atcttttata aattttttat aaaaatcatg agagagaagc
    15661 attatccaaa aagtgtcatc ccataccatt gaaccagctt cttcagtaga aaattcttca
    15721 ttttttttaa aacccagctt ttcataaaaa gcaacggaac gattgacatc agcaacagga
    15781 aaattaacaa aaaccatact attcatttgt ttcgtctcct ttctgttttg ttcgtctatc
    15841 atctcttatt taggaaacgc tgtcaaaaat ttagattcat atatagaaac caaaataaaa
    15901 atttgcatca tcaaaaaagt gaagatgtga tagaatgagg actggttcac tttcacttaa
    15961 ctaatagatg gaatttcttt ataataaaaa agtgaattta tattgaaaaa gaaaaaaaca
    16021 agtaaagccg gggaatattc cggtatgaaa ggaatggaga gaacaatgac catccaatgg
    16081 tttccgggac atatggcaaa agcccgacga gaagtttctg aaaaaatgaa atatgtggac
    16141 atcgtatttg aattggtaga cgcacgatta cctttatctt ctcgtaatcc aatgctagat
    16201 cagatcattc agcaaaaacc tcgccttgtt ttattgaata aagcggatct ggcagatcca
    16261 caacagacaa agctgtggca agaatatttt cgtaaacaag gccatcttgc attagctatc
    16321 acggcacaag aaagtaaagg aattaaaggt ctagtacctg cggctaaaga agcattgaaa
    16381 gaaaaacggg caagagatgc tgctcggggg atcaagcctc gcgctattcg ggcaatggtc
    16441 ttaggtattc caaatgttgg gaaatctacg ttgatgaatc gactagtagg caagaaaatt
    16501 gcacagacag gaaataagcc tggcgtaaca aaaggtcagc agtggcttcg tttaggtaat
    16561 gagttggaat tgttagacac accagggatt ttatggccca aatttgaaga tgaagaaatc
    16621 ggcaaaaaac tagcgctgac aggcgcgatc aaagaccagc ttgtccattt agatgattta
    16681 gcgatttacg gattagactt ttttgcccga tattattcag gaagaatcag cgatcgttat
    16741 ggtttatcaa tcgagcaaga agaaaaattg gcaccggaat tactgatgga tattacagca
    16801 aaacgtgggt ataaagaaga ctatgatcgt ggcagcgaat tagtcgtgca tgatattcgt
    16861 caaggaaaac ttggacgata cacactggat cgttgcgaag aaatggagca aaaaagtgat
    16921 gactgaatcc atccaaagta tcaaagaacg attgaaaagc atacagagaa tagatgatcc
    16981 tacactagaa tcattaagaa atgatccacg caaaggcgtg cagcaagcac tggcttcttt
    17041 tgaacgacga ttgaaaaagg aagcattaat taaggaaaaa tatgaagaaa tgttttgctt
    17101 tgaaaagcaa gggtacgcac aaggtcacca atggattgcc ggcattgatg aagtaggaag
    17161 aggaccatta gctggacctg tcgtggcagc tgcagttatc ttgccgaaaa atgagatcat
    17221 attagggctg aatgactcca aacaattatc agagaaaaag cgagaacttc tttatcaaca
    17281 agttcaagaa aaagccctgg caatcgggat cgggatcgtt gatcagcaga caatcgatga
    17341 tgtgaatatt tatgaagcaa gtaaattagc gatgatccaa gcagtagaaa aattgtccat
    17401 tgtaccaagc tatttgttga tcgatgcgat gaccttggat ttgcctattc agcaggaaaa
    17461 aataatcaaa ggggatgcac gatctgtgtc gattgctgct gcaagtatca ttgctaaagt
    17521 atatcgagac caattgatga aagaatatga cgtgctttat cctggatatg gtttctcgca
    17581 taatgcaggt tatggaacaa aggaacactt agaaggactt gaacgacaag gaattacgcc
    17641 aatccatcgt aaaagttttg cacctgtaaa aaaatattta caataaaaaa cagccgcttt
    17701 tacgtatctt attagaaaga gagacgaaag gcggttttat gatgtatcaa atagaagaaa
    17761 atttattgaa acttgcttat acaaaaggaa tcagtagtca aggaaaatgg gcacttgcta
    17821 actggatgat gcagtatcct tataaagaaa tcggatttga agacatcatt aagataggta
    17881 aaataaccaa acaccgggag ctgtttcgca aaagttggca ggaaattcac gctaactggg
    17941 gcaaaatcca atcgaaacag tcgtttatca cttgctttga tcccaaatat ccccctcagc
    18001 tgcttcactt gacctatccg ccaattgtac tgttttataa tggtgatcta tctttacttt
    18061 cttgtaacat gttaagcgtt gttggtggaa gacacacttc tgctttagcc aaaaaaacag
    18121 tttattattt actaaaacca gtgatagaag ctggatatgt gatcgtcagt ggctgcgcga
    18181 aagggataga tacatacggt caccaagcag caatctctca tggtggacgg acaatcgcag
    18241 tcattggaac agggatagag caagtctatc ctgctgaaaa tcgtgacttg caagaggtga
    18301 ttgcaaatga tcatcttctt ttgagtgaat atcctcctca atccggcccg aaaaagcatt
    18361 gttttccaat gagaaatcgg attatcgcag ctttgtctaa aggaacatgt gtctttgaag
    18421 ccaagaaaaa aagcggctct ctgataacag cacagcaagc attggatctt ggaagaacag
    18481 ttttttctgt tccaggaaat attttaacag aacattctac cggatgtcat catttgatcc
    18541 aagacggtgc agtttgcacg gtttcgggtg aagatatctt ggcagaattt aaagaataac
    18601 accatacttt tcatttgctt ttcttgacaa acgttttctg aatggatatg attagctacg
    18661 atttgtatga atcctctttt aatagtagga acacgacaat ggaaaggagt ccattcagta
    18721 atggcgtata aatatttagt gattgtagaa tcacctgcaa aagccaagac aattgagaaa
    18781 tatcttggac gaaactataa agtaatggcc agtgttggcc atataagaga tttaccgaaa
    18841 agtaaaatgg gtatcgattt tgaaaacaat tatgaacccc attatatttc tatacgcgga
    18901 aaaggcgatg tcatcaaaag cctgaaagcc gcagcaaaaa aagctcaaaa agtttacttg
    18961 gcaagtgacc cggatagaga aggagaagcg attgcttggc atttagcgta cctacttggg
    19021 ttggatctga aagaaaaaaa tcgggtggtc ttcaatgaaa tcacaaaaga cgcagtcaaa
    19081 gcagctttta aggaaccaag aacgatcgat gtagatttag tagatgcaca gcaagctcgt
    19141 cgtaccttag acagaatcgt tggttattcg atcagtccta ttctctggcg taaggtcaag
    19201 aaagggttaa gtgcaggacg tgtccaatct gtcgctttaa aaattattat tgaccgtgaa
    19261 aaagagatcc gagaatttgt tccagaagaa tattggagca tcgacggtaa ttttaaaaaa
    19321 gctcgcaaga aattcaaagc aaatttctgg ggaatcgacg gtaagaaaaa gaaattacca
    19381 gatgcacaaa gtgtaaaaga agtcactgct agaattgatg gaacagactt cgaagttaaa
    19441 aaagtagaaa agaaagagcg caaaagaaac cctgcgaatc ctttcactac aagtagcctg
    19501 caacaagaag ctgcccgtaa gttaaacttt cgaacaagaa aaaccatgat ggtagcacag
    19561 caattatatg aaggaatcac tttgggtaaa caaggaacag tcggcttgat tacatacatg
    19621 cgtactgatt ctacccggat tgccgataca gcaaaagcag aggccgcagc atttattgaa
    19681 gatacgtatg gaaaagaatt ttctagacat gatcaccgaa agatcaaaaa tgctcaaggc
    19741 gcacaagatg cccatgaagc tgtgcgtcca acgagtgtgc ttcgtacacc agatgaactc
    19801 aaacaatatt tagataaaga tcaattgaaa ctttatacat tgatctggtc acgttttgtg
    19861 gctagtcaga tgacaccagc tattttagat acaatgaaag tgacattaga acaaaatggt
    19921 gtcatcttca ttgcgaacgg ttcaaaaatc aaattcaaag gattcatgca agtgtatgtg
    19981 gaaggtagag atgacggcaa agaagagaaa gaaaatattc ttccagaact tgaagaaggc
    20041 gatgtagtcc aatctgttga tattgaacca aaacaacatt ttactcaacc accagcacgt
    20101 tttagtgagg ctacgttgat tcgtgcacta gaagaaaacg gggtagggcg accttccact
    20161 tatgcaccaa cacttgatac gatccaaaga cgctactatg taaaattgac acaaaaaaga
    20221 tttgagccga ctgaactagg agaaattgtc aattcattaa tctgtgaatt cttccctcaa
    20281 atcgtcgata tccatttcac agcagaaatg gaaggagatt tggataaaat tgaagaaggc
    20341 acggaagcgt gggtcaaagt ggttgatcgt ttctataaac cgtttgaaaa agaactgacg
    20401 aatgcggaag aaaaaatcga aaaaattcaa atcaaagatg aaccagcagg attcgattgt
    20461 gatgtctgtg gacatccgat ggtgataaaa ttagggaaat atgggaagtt ttatgcttgc
    20521 agtaattttc ctgattgtcg gaatacgaaa ccaatcgtaa aagaaatcgg tgtgacttgc
    20581 ccagtatgtc atgaaggaca agtcattgaa cgaaaatcta agaaaaacag actattctat
    20641 ggttgttccc gtttcccaga atgtgaattc acttcttggg ataaaccagt gggcagaaat
    20701 tgtccaaaat gtgacggata tctggtcgag aagaaagtca aaggcggtaa acaagtagtc
    20761 tgtgtaaatg gagattacga agaagacatc caaaaataat agaaaagagg ttctttcatt
    20821 gaaaatggta aatgttgttg gagcagggct tgccggaagt gaagctgctt ggcagatcgc
    20881 acaagcagga gtccctgtcc gtttatatga aatgcgtcca gtcaaatcga ctgaggcgca
    20941 tcacacaagt aattttgctg aactagtatg ttccaattca ttaagaggaa acagtttggc
    21001 taatgcagta gggttattga aagaagaaat gcgccgattg aattcggtag taatcacttc
    21061 ggcagatgaa acagcagtgc cagctggtgg tgccttagct gttgaccgcg attctttttc
    21121 tgaaacaatt acccgaaaag tcaaagagca tccattagta acagtgataa acgaagaaat
    21181 tactgaaata ccggaaggaa tcaccgtagt agcaacagga cctttgactt ctcatccatt
    21241 agcagaaagc atcaaagcat tcaatggttc agatggtttt tatttctatg atgcagctgc
    21301 accaatcgta gacaaagcta cgatcgatat ggataaagta tatttgaagt cccgctatga
    21361 caaaggagaa gcagcttact tgaactgtcc gatgacagaa gaggaattca aagctttcca
    21421 tgaggcacta atcaatgccg aagtggcaga acttaaatct tttgagaaag aaaaatattt
    21481 tgaaggatgt atgccaattg aagtaatggc acagcgagga ttcaaaacga tgcttttcgg
    21541 acctatgaaa ccagttggat tggaagatcc taaaactggc aaacgtcctt atgctgttat
    21601 ccagctgcgt caagacaatg ctgcagcttc tttatacaac atcgtgggct ttcagactca
    21661 cttgaaatgg ggcgagcaaa aacgtgtttt ccggatgatt cctgggttag aaaatgctga
    21721 attcgttcgc tatggtgtga tgcaccgaaa cagctttatg aattcgccag aattgctgaa
    21781 accgacttac caatcaacga aacgcgaaga tctgttcttt gcaggtcaga tgactggtgt
    21841 agaaggctat gttgaaagtg cagcgagcgg actacttgct ggaatcaacg ccgcacgctt
    21901 agcaaaagaa atggaaccag tcgagtttcc acaagaaaca gctattggaa gtatggctta
    21961 ctacattact catgcggaag gaaaacattt ccaaccgatg aatgccaact atggtctgtt
    22021 tcctgaactg ccagagcgca ttcgagacaa aaaagcaaga tatgaagcaa ttgcacaacg
    22081 agctttaaaa gctaatgagg caataaaaac agaattagaa aaaactgcaa tcccaaatga
    22141 gtaaggaaac tccatgaaga gggtgagacg gatagtctcg ctctttttct tgcgtttttc
    22201 ttaagaattt ctcaaacaaa atgtgaggaa ttgcgccaat tgtgaacaaa cttagaaata
    22261 ttggttaaat tctgacaatt tatttgtctt tttataaacg ctgtgctaaa ttattctctg
    22321 ggacaaaagg aaggtgagaa aatgacagaa gccacgtggg aacaaaaatt tttgcgttat
    22381 ttgattgtcg aaagaggata ttcagaaaaa acgaaagaag catatgaaga agacattctc
    22441 catttcaaac attttttgga ggaatctgga gacgctgatc tgctgaaggc ggaatatttt
    22501 acggttcgtg tctatttgag tgcattagct gatcatcgtc ctagctatag cagaaattct
    22561 atcagtcgca agatagccag cctacgttcg ttttatcagt atcttctcaa agaagaagtg
    22621 ataaaagaaa atccattttc ttacgttcat ctcaaaaaga aaaatttgag actgccacgt
    22681 tttttttatg aaaatgaaat gcaagcactt tttgacagcg tagcaggaga tagtcctttg
    22741 gatttgagga accgcgcttt actggaagtc ctttacgggt caggcatccg tttaagtgag
    22801 tgtagcagtt tgtcggtatc agacattgat tttgattcag aggtcatgtt gatccatggt
    22861 aaagggaaca aagagagata tgctcctcta ggttcttttg ctcaagatgc tctgcaagaa
    22921 tatttcaggg aaggccgcca ggtattgatg acaaaatatc acgaagagca tgatatcgtt
    22981 tttgtgaatc atcatggaaa accaatcacg cctactggaa ttgaatatgt cttgaatcaa
    23041 atgatcaaaa aaagcagttt ggatagtgat atccatcctc acatgctgcg ccatacattt
    23101 gctactcatt tattaaataa tggggcagat ttgcggacag tccaagaatt gctaggacat
    23161 gccaatttat cgactaccca gatctatgcc catgtaacaa aagaaagttt acaaaaaaac
    23221 tatcgttctt tccatccaag agcgtaaaaa taaggaaaag aacaaattta ggaggaaaca
    23281 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
    23341 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
    23401 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
    23461 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
    23521 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
    23581 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
    23641 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
    23701 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
    23761 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
    23821 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
    23881 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
    23941 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
    24001 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
    24061 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
    24121 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
    24181 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaatgc agaaaaacgt
    24241 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
    24301 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
    24361 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
    24421 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
    24481 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
    24541 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
    24601 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
    24661 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
    24721 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
    24781 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
    24841 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
    24901 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
    24961 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
    25021 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
    25081 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
    25141 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
    25201 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
    25261 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
    25321 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
    25381 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
    25441 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
    25501 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
    25561 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
    25621 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
    25681 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
    25741 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
    25801 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
    25861 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
    25921 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
    25981 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
    26041 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
    26101 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
    26161 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
    26221 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
    26281 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
    26341 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
    26401 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
    26461 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
    26521 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
    26581 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
    26641 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
    26701 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
    26761 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
    26821 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
    26881 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
    26941 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
    27001 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
    27061 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
    27121 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
    27181 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
    27241 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
    27301 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
    27361 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
    27421 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
    27481 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
    27541 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
    27601 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
    27661 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
    27721 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
    27781 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
    27841 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
    27901 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
    27961 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
    28021 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
    28081 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
    28141 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
    28201 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
    28261 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
    28321 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
    28381 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
    28441 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
    28501 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
    28561 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
    28621 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
    28681 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
    28741 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
    28801 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
    28861 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
    28921 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
    28981 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
    29041 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
    29101 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
    29161 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
    29221 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
    29281 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
    29341 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
    29401 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
    29461 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
    29521 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
    29581 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
    29641 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
    29701 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
    29761 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
    29821 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
    29881 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
    29941 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
    30001 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
    30061 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
    30121 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
    30181 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
    30241 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
    30301 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
    30361 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
    30421 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
    30481 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
    30541 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
    30601 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
    30661 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
    30721 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
    30781 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
    30841 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
    30901 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
    30961 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
    31021 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
    31081 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
    31141 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
    31201 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
    31261 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
    31321 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
    31381 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
    31441 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
    31501 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
    31561 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
    31621 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
    31681 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
    31741 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
    31801 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
    31861 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
    31921 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
    31981 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
    32041 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
    32101 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
    32161 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
    32221 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
    32281 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
    32341 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
    32401 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
    32461 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
    32521 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
    32581 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
    32641 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
    32701 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
    32761 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
    32821 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
    32881 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
    32941 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
    33001 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
    33061 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
    33121 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
    33181 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
    33241 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    33301 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    33361 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    33421 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    33481 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    33541 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    33601 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    33661 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    33721 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    33781 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    33841 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    33901 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    33961 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    34021 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    34081 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    34141 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    34201 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    34261 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    34321 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    34381 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    34441 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    34501 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    34561 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    34621 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    34681 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    34741 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    34801 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    34861 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    34921 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    34981 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    35041 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    35101 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    35161 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    35221 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    35281 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    35341 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    35401 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    35461 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    35521 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    35581 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    35641 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    35701 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    35761 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    35821 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    35881 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    35941 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    36001 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    36061 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    36121 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    36181 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    36241 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    36301 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    36361 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    36421 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    36481 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    36541 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    36601 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    36661 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    36721 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    36781 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    36841 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    36901 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    36961 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    37021 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    37081 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    37141 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    37201 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    37261 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    37321 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    37381 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    37441 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    37501 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    37561 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    37621 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    37681 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    37741 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    37801 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    37861 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    37921 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    37981 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    38041 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    38101 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    38161 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    38221 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    38281 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    38341 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    38401 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    38461 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    38521 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    38581 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    38641 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    38701 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    38761 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    38821 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    38881 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    38941 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    39001 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    39061 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    39121 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    39181 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    39241 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    39301 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    39361 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    39421 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    39481 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    39541 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    39601 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    39661 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    39721 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    39781 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    39841 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    39901 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    39961 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    40021 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    40081 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    40141 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    40201 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    40261 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    40321 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    40381 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    40441 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    40501 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    40561 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    40621 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    40681 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    40741 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    40801 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    40861 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    40921 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    40981 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    41041 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    41101 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    41161 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    41221 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    41281 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    41341 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    41401 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    41461 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    41521 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    41581 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    41641 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    41701 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    41761 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    41821 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    41881 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    41941 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    42001 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    42061 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    42121 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    42181 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    42241 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    42301 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    42361 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    42421 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    42481 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    42541 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    42601 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    42661 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    42721 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    42781 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    42841 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    42901 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    42961 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    43021 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    43081 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    43141 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    43201 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    43261 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    43321 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    43381 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    43441 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    43501 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    43561 taaataagtg atatacaaat ttatattcgg ctacagttgg aaagg
//
LOCUS       DACDOA010000012        42736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDOA010000012 DACDOA010000000
VERSION     DACDOA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.012"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            352..1041
                     /locus_tag="IWI39_000585"
     CDS             352..1041
                     /locus_tag="IWI39_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9207713.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IWI39_000586"
     CDS             1028..2236
                     /locus_tag="IWI39_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9207714.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGISTIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELKASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IWI39_000587"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IWI39_000587"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9207715.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IWI39_000588"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IWI39_000588"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9207716.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IWI39_000589"
     CDS             complement(6339..7034)
                     /locus_tag="IWI39_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9207717.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IWI39_000590"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IWI39_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9207718.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IWI39_000591"
     CDS             complement(8233..8424)
                     /locus_tag="IWI39_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9207719.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IWI39_000592"
     CDS             complement(8445..9335)
                     /locus_tag="IWI39_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9207720.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IWI39_000593"
     CDS             complement(9322..10089)
                     /locus_tag="IWI39_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9207721.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IWI39_000594"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IWI39_000594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9207722.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IWI39_000595"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IWI39_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9207723.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IWI39_000596"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IWI39_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9207724.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IWI39_000597"
     CDS             14705..15043
                     /locus_tag="IWI39_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207725.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IWI39_000598"
     CDS             complement(15211..15969)
                     /locus_tag="IWI39_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9207726.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IWI39_000599"
     CDS             complement(15987..16805)
                     /locus_tag="IWI39_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9207727.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IWI39_000600"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IWI39_000600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9207728.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IWI39_000601"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IWI39_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9207729.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="IWI39_000602"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="IWI39_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9207730.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..20596
                     /gene="dnaN"
                     /locus_tag="IWI39_000603"
     CDS             19466..20596
                     /gene="dnaN"
                     /locus_tag="IWI39_000603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9207731.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20825..21067
                     /gene="yaaA"
                     /locus_tag="IWI39_000604"
     CDS             20825..21067
                     /gene="yaaA"
                     /locus_tag="IWI39_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9207732.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21054..22178
                     /gene="recF"
                     /locus_tag="IWI39_000605"
     CDS             21054..22178
                     /gene="recF"
                     /locus_tag="IWI39_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9207733.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22175..24121
                     /gene="gyrB"
                     /locus_tag="IWI39_000606"
     CDS             22175..24121
                     /gene="gyrB"
                     /locus_tag="IWI39_000606"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9207734.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24141..26612
                     /gene="gyrA"
                     /locus_tag="IWI39_000607"
     CDS             24141..26612
                     /gene="gyrA"
                     /locus_tag="IWI39_000607"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9207735.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26848..27147
                     /locus_tag="IWI39_000608"
     CDS             26848..27147
                     /locus_tag="IWI39_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9207736.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27197..27709
                     /gene="ssb"
                     /locus_tag="IWI39_000609"
     CDS             27197..27709
                     /gene="ssb"
                     /locus_tag="IWI39_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9207737.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27735..27971
                     /gene="rpsR"
                     /locus_tag="IWI39_000610"
     CDS             27735..27971
                     /gene="rpsR"
                     /locus_tag="IWI39_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9207738.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28114..30087
                     /locus_tag="IWI39_000611"
     CDS             28114..30087
                     /locus_tag="IWI39_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9207739.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="IWI39_000612"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="IWI39_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9207740.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="IWI39_000613"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="IWI39_000613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9207741.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="IWI39_000614"
     CDS             32966..34258
                     /locus_tag="IWI39_000614"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9207742.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35045..35908
                     /locus_tag="IWI39_000615"
     CDS             35045..35908
                     /locus_tag="IWI39_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9207743.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36037..37659)
                     /locus_tag="IWI39_000616"
     CDS             complement(36037..37659)
                     /locus_tag="IWI39_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9207744.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37908..38429
                     /locus_tag="IWI39_000617"
     CDS             37908..38429
                     /locus_tag="IWI39_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207745.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38615..40345)
                     /gene="spxB"
                     /locus_tag="IWI39_000618"
     CDS             complement(38615..40345)
                     /gene="spxB"
                     /locus_tag="IWI39_000618"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9207746.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40510..41328
                     /locus_tag="IWI39_000619"
     CDS             40510..41328
                     /locus_tag="IWI39_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9207747.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41431..41628)
                     /locus_tag="IWI39_000620"
     CDS             complement(41431..41628)
                     /locus_tag="IWI39_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207748.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41797..42375
                     /locus_tag="IWI39_000621"
     CDS             41797..42375
                     /locus_tag="IWI39_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9207749.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        12984 a         8197 c         8457 g        13098 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcagc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttga aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20641 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt
    20701 tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt
    20761 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
    20821 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
    20881 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
    20941 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
    21001 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
    21061 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
    21121 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
    21181 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
    21241 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
    21301 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa
    21361 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
    21421 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
    21481 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
    21541 atttaaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
    21601 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
    21661 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
    21721 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
    21781 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
    21841 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
    21901 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
    21961 ttaaagaaga gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
    22021 atgatagtcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
    22081 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
    22141 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
    22201 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
    22261 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
    22321 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
    22381 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
    22441 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
    22501 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
    22561 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
    22621 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
    22681 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
    22741 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
    22801 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
    22861 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
    22921 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
    22981 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
    23041 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
    23101 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
    23161 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
    23221 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
    23281 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
    23341 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
    23401 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
    23461 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
    23521 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
    23581 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
    23641 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
    23701 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
    23761 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
    23821 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
    23881 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
    23941 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
    24001 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
    24061 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
    24121 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
    24181 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
    24241 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
    24301 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
    24361 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
    24421 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
    24481 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
    24541 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
    24601 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
    24661 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
    24721 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
    24781 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
    24841 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
    24901 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
    24961 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
    25021 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
    25081 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
    25141 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
    25201 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
    25261 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
    25321 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
    25381 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
    25441 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
    25501 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
    25561 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
    25621 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
    25681 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
    25741 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
    25801 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
    25861 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
    25921 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
    25981 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
    26041 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
    26101 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
    26161 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
    26221 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
    26281 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
    26341 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
    26401 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
    26461 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
    26521 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
    26581 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
    26641 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
    26701 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
    26761 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
    26821 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
    26881 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
    26941 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
    27001 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
    27061 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
    27121 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
    27181 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
    27241 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
    27301 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
    27361 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
    27421 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
    27481 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
    27541 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
    27601 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
    27661 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
    27721 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
    27781 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
    27841 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
    27901 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
    27961 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
    28021 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
    28081 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
    28141 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
    28201 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
    28261 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
    28321 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
    28381 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
    28441 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
    28501 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
    28561 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
    28621 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
    28681 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
    28741 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
    28801 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
    28861 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
    28921 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
    28981 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
    29041 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
    29101 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
    29161 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
    29221 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
    29281 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
    29341 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    34381 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34441 ttttccgaac aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa
    34501 aaatatggag tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc
    34561 acaacctctc cactaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc
    34621 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct aaacggcagg
    34681 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    34741 ggcaaaatat gaggaactat tttttccgtt tttttctcta atactcaggt caaaacgctg
    34801 atttcacaac ctctgattca cggtgttcca gaagtagctt ctacggaaat aagccgaaat
    34861 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    34921 ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa taaaccggtt aaagtgtgtc
    34981 tagcgcaaga gatttgctat aatagacgat aagggtaaaa tgtaacgaga aggggttgaa
    35041 aaatgtgtat caaattgtaa cagattcttg ttgtgatctg ccataccatg tattagcaaa
    35101 agaaaaagtt gattttttat caatgaatct ccaattgaat gatcaagagt tagtagatga
    35161 cctcggaaag acattcaatt atgaccgcta catgaatcag ctgaaaaatg gtgcgatgcc
    35221 tacgacatca caaatcaatg ttggccgata tttagaattt ttccgttctt atgcggaaaa
    35281 acagattcct gttttatatg tggctttttc cagtggatta agcggctctt acagtagtgc
    35341 gcttcaagcg gttgaattgc tgaaggaaga atatgaagac cctgagatct atgtttttga
    35401 tacaaaagct gcaagtattg gggaagggct tttagtattg gaagctgtaa gattgaaaaa
    35461 agaaggtaaa agcatcgaag aaaccctaga gtggctggaa agtaattatt tgaaagtcca
    35521 ttcatgggta acagtagacg atctgaaata tctagaacga ggcggtagga tttccaaagc
    35581 aagtgctact cttggcagtc tattgagtgt aaagcctatt attacggtgg ataaagaagg
    35641 aaggctgacg aatgttgaca aagttcgagg aagaaacaag tcgatccaga caatcgtcaa
    35701 tgagacggta aaaacaatcg ttgcaccact tgatcaaatc gtttatgtgg cttacgctgg
    35761 ggatctagag agcgcggaga aggcaaaacg gcttttggaa gagcagataa agatgaaaga
    35821 tgtgaagctt tatcccttag gaccaacgat cgccagtcac acaggctatg gctgtatcgc
    35881 gattttttcg atgggaatca gtcgataaaa gcagtaactc attctgtaaa aaagcccaga
    35941 aattgattgt aacaaaaaca gcgactctta aaagatagat gagcatccta tctcttagag
    36001 gtcgctgttt ttttattttt taagctaaat aggccattat gcccataact cgttcaattg
    36061 tttttgaata tctgcatttt ccaaaaattc atcatatgtc gtatcagctc gatcgatcac
    36121 acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt tggataaatt gatggtcatg
    36181 tgacgcaaat aagattgagc cagtaaatgc catcaagccg tcattcaatg ctgtgattga
    36241 ttctaagtct aaatggttcg ttggatcatc taatacaagt acgttagctt tcgaaagcat
    36301 catttttgaa agcatgcaac ggactttctc tccaccagat aaaacattca ctggtttcaa
    36361 tacttcttca ccagaaaaca acattcttcc taagaagctt cttaagaagg tgttatcatc
    36421 ttcttcttta cttgcatatt gacgcaacca gtctaaaatc gttagatcag tatcgaattc
    36481 tttcgttgta tcctttggta aataagaatt acttgttgtc acaccccaac gtactgttcc
    36541 agtatctggc gtaatttcac ccatgataac tttgaatagc gttgttgttg ttatatcatt
    36601 ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta aaagatatat catctaaaat
    36661 tttctttcca tcaatcgtta cacttacatt ttctacctgc aataagtcgt ttccaatctc
    36721 acgttcaggt ttgaatccaa cgaatggata acgtctagaa gaaggttgga tatcgtctaa
    36781 agtaattttt tcaagcattt ttttacgtga agttgcttgt tttgattttg atgcattcgc
    36841 actaaagcgg gcaataaatt cttggagttc tttgatttgt tcttcttttt tggcatttgc
    36901 gtttgcttga agtttagccg ctagttgact agattccaac caaaaatcat aatttcctac
    36961 ataaagtttg attttaccaa aatctagatc cgccatatgt gtacatactt tgttcaaaaa
    37021 gtgacggtca tgggatacaa cgatcacagt attttcaaaa ttgatcaaaa attcttccag
    37081 ccaattgatc gattggatat ccagaccgtt tgttggttcg tctagcaaaa gaacatcagg
    37141 tttaccaaat agtgtttgtg ctaataacac ttttaccttt tgtccaccag ttaattcact
    37201 catttgctga tggtgcaatt cttcagggat gttcagacct tgcagtaaaa cagctgcttc
    37261 tggttcagct tcccatccat taagctcagc aaattctcct tctaattcag ctgctcgaat
    37321 accatcttcg tcagtgaaat cctctttcat gtaaatcgca tctttttctt tcagcacttc
    37381 atacagacgc ttgtgtccca tgatgactgt ttcaagtaca gtatagtctt catagtcgta
    37441 atggttctgt ttcaatacgg ccatccgttc atctggtccc atagtaacgg aacctgtgga
    37501 aggctgcaga tcccctgata acactttaag gaacgttgat ttacctgcac catttgcacc
    37561 aatcaagcca tagcagtttc cgggggtaaa tttaatattc acatcatcaa atagtttgcg
    37621 gtctgaaaag tgcaaactca catcatttac agtaatcaat ttttttcctc acttttatca
    37681 ttattagttt tgacaagtct tatagaatta tataggggaa aggtaaaaga ttcaaggcaa
    37741 tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa aataaaaata aaagcggagc
    37801 gaaaatagct actataggat ttattagttt gcaacagcat aatttttgac gttatttata
    37861 agagggagta ctataaaact gtaaaagaaa tgaaagaagg attgaaaatg gatgaaaaaa
    37921 gaaccatcga ctggggctca ttgattttgg gaattttatt tgtacttgtc tcattgttat
    37981 catttcaaga ccctgtcgga aatttagttg caattgtagt agtctttgcg atttttgcct
    38041 ttattaaagg gatatttgaa ctgtttgtac ggaacagatt aaaagagcta acaggatata
    38101 aaggaaagat gcccttaata atcgggatca tcgatattct agttggcgtc tttttcttat
    38161 tcaatattgg tgcaggagtc gcagcattgc cttttgtttt tgcagtatgg tttattgctg
    38221 attcagtttt agcgttattt actgctgact tggcaagagg agtaagtgaa ggatattatt
    38281 ggtttacgat catcgtcaat attttaggaa ttttattagg aattttctta ttgtttaatc
    38341 caatttcttc tgcgttgaca ttaagctttt tagttggatt ctattttatg ttatttggta
    38401 tcacacatat cgtctatgca tttagataag taaaatcaaa caaataaaaa agccggcttc
    38461 cgattacagt tttactgttg taattggaag ccggcttttt tatcatggac aaatatgcac
    38521 gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat gtgagaagct atatacacaa
    38581 attttatctt aatactcgaa gcatagcgca gttgtcacaa cctcttttca tataattcat
    38641 tcaatactgg caaatcatgg acttcatatt tttctttgaa tgcttggatt tcttcttcag
    38701 aatattttct tggatctaaa tgcagatctt ctactggcaa tgggcgcttg ttattgatct
    38761 tcacatcgat tactacagga cgatctgatt ttgcagctgc atcaaaggca aatcgaagtt
    38821 gcgagtattc tgtgactgta aagctatttg ccccgagcgc ttctccaact ttaccgaaat
    38881 ctgcatcttc taaaaataca ccaaatttct tttgttccgt atcttcttgt tctgcttcga
    38941 tgaaaccaaa agaatcattc gaaaagacaa cattaataat tggaagctga tatttgactt
    39001 gtgtaatgat atcttgcatg accatagcaa acccgccatc accacttaag gtaaatactt
    39061 gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg cacaccatta cccatagttg
    39121 cgaaccatcc agatgtcgta tgtttttgtt taccgttcat attcaataaa cgaatagaat
    39181 ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc gtcttcagca atacgattga
    39241 tttctttata gacagcctct ggacgaatcg gttcttcctc tcgatcttca aaagatttca
    39301 accaatttac ccatttttct ttattttctt gatttgctag gtaccagcta tcagcagggc
    39361 gagcatcccc taactcgaca agtctttgca aagctgtgtt tgcatcaccg aggatcgaca
    39421 aagatgtttc atggcgtcgt ccaaattttg acgcatcgat atcgatttgg ataaattcag
    39481 cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct accgacaaat aagatcagat
    39541 cggcttctgc caatgcttca tttccgggtt tagtagcgac acgtccagca aaaccaagaa
    39601 agttttcata atcatcagga ataattcctt ttgctagaac ggaagcgaca acaggaactg
    39661 aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt tcgcgttcct tgaccaacat
    39721 aaagaactgg tttttttgct ttttcaagat aaggcaaagc agctagcaaa tctttttctt
    39781 ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga gaaaggttgt tcgtcaattt
    39841 cttcaaaacc aagatcaaca gggatagtca cgatagccac gcctttatgc tcataggcgg
    39901 ctttaatggc ttcatctact acatgcggaa gactttgtgg tgtcatgact gtacgattgt
    39961 atacgctaac atctgcgaaa ataggatttt catttagttc ctggaaagag ttatagttca
    40021 tggatgtaga agcaacttgt cctaagaacg ctactagagg cacatggtcc atctgtgcat
    40081 catacaaacc attgatcaaa tgtgtcgcac caggtcctgc agagccgaag acagcgccga
    40141 ctttccccgt taattttgca tctgcagcgg ctgcgagtgc gccgacttct tcatgacgaa
    40201 cttgaatgta gttgatttcg gttttttctt gatatagcgc atccataatt gaattgaaag
    40261 atcctccggg aatgccgtaa atatggtcga taccccagct ttcaagaact tttaccattg
    40321 cgattcctgc attgattttt gacatggtgt ttcactcctt tagcaatgat gatacataaa
    40381 ttttttgtat ctagagtata gaacttttta tcgaaaatcc caactaattc gtcttttcac
    40441 aaaaatgtag tcatgttttt gggaatatgc tagagtagta catagataga ttcaagagag
    40501 gggaatggca tggttgaatg gctattgaga cttgaacctt ggcagcaagc acttgctggg
    40561 acagctttta cttattttat gacagctctt ggtgccgggt tggttttttt ctttaaagaa
    40621 atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat gattgctgca
    40681 agtttttggt ctttgttaga tccggctatt acaaaagcag aagaaaatgg agatattgct
    40741 tggctagttg taagtattgg gtttggttta ggcggtgtgt ttttatatat ggcagataaa
    40801 acactgcccc atatgcattt tggaccacaa catgaagcag aaggattgcc tacccattta
    40861 aaacggacga tcttgcttgt tttttctatt acattgcata acattcctga aggtttagcc
    40921 gtgggggtag cattcggtgc agcagcaact gcggataatc cgacagccgc tatattagcc
    40981 gctgtttcgg tggcgctagg tattggtatc caaaacttcc cagaaggagc agctgtatca
    41041 atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg acaagcttct
    41101 ggaatcgttg aaccaattgc tggagtgatt ggagcgctac tagtaacgaa agtggagctg
    41161 ctgcttcctt atgcacttgc ttttgccgca ggagcaatga tttatgtcgt ggtggaagaa
    41221 ttgatccctg aagcacagca gacattgtcc agcaaacatc attttgcagt ttttggcgtc
    41281 atgagtggat ttatcattat gatgattttg gatgtggcat taggataaat aaaaaaaata
    41341 tatatgatat atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt
    41401 atatcataat gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc
    41461 tttgatccag cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa
    41521 ccataaaagc ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag
    41581 cactaaaaga accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc
    41641 tcttgttcat tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac
    41701 tgaagactga tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt
    41761 atcatctaaa atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca
    41821 tttacagatg ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct
    41881 aacggagaca cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc
    41941 agttttatca ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat
    42001 aagataaatg gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt
    42061 ccgtttgcaa cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat
    42121 ggttttgtta ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa
    42181 gcaaataaag gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat
    42241 ctatcgattg gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc
    42301 gtgctgatcg caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa
    42361 tcacaatatc ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa
    42421 ataaacgatc aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa
    42481 aagaattcat gtaggactga ttactagttt tgtcacgatg cttatttatt tggatcaagg
    42541 tcatttgact tggacagtag ttggtgtcgt aggatttttg acattattca cagcaataaa
    42601 aaaataaaca tctttatgta atctgatgtt ctatctattt aagaaaagca tgtttacagc
    42661 agtatgattc tgttgtaaac gtgtttttat ttgtggaaaa tttaattttt agaaaagtgt
    42721 atcgaaaaat agatag
//
LOCUS       DACDOA010000013        38954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDOA010000013 DACDOA010000000
VERSION     DACDOA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.013"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(19..1773)
                     /gene="ade"
                     /locus_tag="IWI39_000622"
     CDS             complement(19..1773)
                     /gene="ade"
                     /locus_tag="IWI39_000622"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9207750.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            1905..2834
                     /gene="cpaF"
                     /locus_tag="IWI39_000623"
     CDS             1905..2834
                     /gene="cpaF"
                     /locus_tag="IWI39_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9207751.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            2791..3840
                     /locus_tag="IWI39_000624"
     CDS             2791..3840
                     /locus_tag="IWI39_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9207752.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            3837..4115
                     /locus_tag="IWI39_000625"
     CDS             3837..4115
                     /locus_tag="IWI39_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9207753.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            4090..4560
                     /locus_tag="IWI39_000626"
     CDS             4090..4560
                     /locus_tag="IWI39_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9207754.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            4577..4879
                     /locus_tag="IWI39_000627"
     CDS             4577..4879
                     /locus_tag="IWI39_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207755.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            4896..5267
                     /locus_tag="IWI39_000628"
     CDS             4896..5267
                     /locus_tag="IWI39_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9207756.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            5239..5598
                     /locus_tag="IWI39_000629"
     CDS             5239..5598
                     /locus_tag="IWI39_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207757.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            5770..6777
                     /locus_tag="IWI39_000630"
     CDS             5770..6777
                     /locus_tag="IWI39_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9207758.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            6802..7986
                     /locus_tag="IWI39_000631"
     CDS             6802..7986
                     /locus_tag="IWI39_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9207759.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(8423..10006)
                     /locus_tag="IWI39_000632"
     CDS             complement(8423..10006)
                     /locus_tag="IWI39_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9207760.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(10584..10727)
                     /locus_tag="IWI39_000633"
     CDS             complement(10584..10727)
                     /locus_tag="IWI39_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9207761.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(10950..11429)
                     /locus_tag="IWI39_000634"
     CDS             complement(10950..11429)
                     /locus_tag="IWI39_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9207762.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(11502..11786)
                     /locus_tag="IWI39_000635"
     CDS             complement(11502..11786)
                     /locus_tag="IWI39_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207763.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(11864..12058)
                     /gene="ssrS"
                     /locus_tag="IWI39_000636"
     ncRNA           complement(11864..12058)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWI39_000636"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(12130..13416)
                     /locus_tag="IWI39_000637"
     CDS             complement(12130..13416)
                     /locus_tag="IWI39_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9207764.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            13554..14225
                     /locus_tag="IWI39_000638"
     CDS             13554..14225
                     /locus_tag="IWI39_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9207765.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            14191..14679
                     /locus_tag="IWI39_000639"
     CDS             14191..14679
                     /locus_tag="IWI39_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9207766.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            14692..15639
                     /gene="prmA"
                     /locus_tag="IWI39_000640"
     CDS             14692..15639
                     /gene="prmA"
                     /locus_tag="IWI39_000640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9207767.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            15640..16404
                     /locus_tag="IWI39_000641"
     CDS             15640..16404
                     /locus_tag="IWI39_000641"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9207768.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            16637..18850
                     /locus_tag="IWI39_000642"
     CDS             16637..18850
                     /locus_tag="IWI39_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9207769.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            18861..19307
                     /locus_tag="IWI39_000643"
     CDS             18861..19307
                     /locus_tag="IWI39_000643"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9207770.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            19478..20737
                     /locus_tag="IWI39_000644"
     CDS             19478..20737
                     /locus_tag="IWI39_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9207771.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(20826..21773)
                     /locus_tag="IWI39_000645"
     CDS             complement(20826..21773)
                     /locus_tag="IWI39_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9207772.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            22167..23459
                     /locus_tag="IWI39_000646"
     CDS             22167..23459
                     /locus_tag="IWI39_000646"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9207773.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            23474..25243
                     /gene="aspS"
                     /locus_tag="IWI39_000647"
     CDS             23474..25243
                     /gene="aspS"
                     /locus_tag="IWI39_000647"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9207774.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            25352..26227
                     /locus_tag="IWI39_000648"
     CDS             25352..26227
                     /locus_tag="IWI39_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9207775.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            26493..28376
                     /locus_tag="IWI39_000649"
     CDS             26493..28376
                     /locus_tag="IWI39_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9207776.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(28451..28735)
                     /locus_tag="IWI39_000650"
     CDS             complement(28451..28735)
                     /locus_tag="IWI39_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9207777.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(28725..29459)
                     /locus_tag="IWI39_000651"
     CDS             complement(28725..29459)
                     /locus_tag="IWI39_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9207778.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            29588..30211
                     /locus_tag="IWI39_000652"
     CDS             29588..30211
                     /locus_tag="IWI39_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9207779.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            30403..31524
                     /locus_tag="IWI39_000653"
     CDS             30403..31524
                     /locus_tag="IWI39_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9207780.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            31531..34659
                     /locus_tag="IWI39_000654"
     CDS             31531..34659
                     /locus_tag="IWI39_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9207781.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            34678..37872
                     /locus_tag="IWI39_000655"
     CDS             34678..37872
                     /locus_tag="IWI39_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9207782.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(38018..38725)
                     /locus_tag="IWI39_000656"
     CDS             complement(38018..38725)
                     /locus_tag="IWI39_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9207783.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        12619 a         6984 c         8235 g        11116 t
ORIGIN      
        1 ttctgggatt tatttgattt aaaatgtatt tatttcatcg gtttttccac aaggtggaat
       61 caattggaag cgagaaaatg aaaataatcc tttgtcggtg atcttcaatt cgggtatgac
      121 agataatgtt aagaaagaaa gtgtcaaaaa aggatcaaag gttttagctg ctcctagttg
      181 atgtgcttta gttattaatt cattcaactg ttcatagacg atcgaaaaag gttcctgtgt
      241 catgattccg cctataggaa gagaaacaca agctatttcc tgctgattgt tgacaacggt
      301 catcccgcca cctttttgaa tcaaaagatt agcagcatac agcatatcct cgtcgtttgt
      361 cccaacgaca actagattat gcgagtcatg ggcaatcgtt gtcgctatcg ctccttgttt
      421 cagctgaaat cctttcacta tgcctaatcc aatgtttccc gtttgatggt gacgctcgat
      481 caccgcaatc tttaattggt ctttttcgat tgaaggaatg aatcgttcgc cttgtcggtc
      541 cactttttct accaaatctt tagtcactag actattcggg ataatttcga tcacatgggc
      601 ttgtgaagaa gaaagaagca gctctaacga atgtggattt aatggttgag cgtttagttt
      661 tggcagacaa cacgtatatg aatttgaatg atcagcaaat aaatggtctt ggagttgccc
      721 agattgaaca actaatgttc ccttatagaa aacttcatga attggcagct tggtaagatc
      781 gtctgttaag aagaaatcag cttgataccc aggggcaatc gcacctagtg ttttcaaatc
      841 aaaacattct gctgcattca atgttgccat ctgaatggct tgtaatggat cgattccaag
      901 tgcaatagct tgacggacga tatgatcgat tgatccttct tttactaaat cattgatcag
      961 tttatcatcg gtgacaaaga agcatcttct tgaattttca ggtgtgatcg ccggtagtag
     1021 cgatgccaga tcttttgcaa cggtcccttc tcgaaccatt aaatacatac caagatccaa
     1081 acgttcttta gcttcttttg cagttgtagc ttcatgatcc gtccgaatac ctgctgcagg
     1141 ataaacattt aactcttcca tgccgatacc agctgcatgc ccgtcaattt ttttcttatg
     1201 ctggtgcatc aattggatct tttctaggat gtctgtttca ttatttgcta ctgcttgata
     1261 gttcatgact tcagctaagc ctaaaacttc gggtttttgt aaaaaagggg ctaatttttt
     1321 tgcatttaaa tttgcaccat tggtctcaaa aggggttaca ggtacacaag aaggtagcat
     1381 aaaaaagata ttcatgagtg tttgtcttgc atcttctatc ataaactcaa gacctgtact
     1441 cccagcaatg tttcctattt catggggatc tgtcactaca gtagtaactc catgaagcaa
     1501 ggatactttt gaaaattcgc ttggtgataa aagtgagctt tcgatatgca catggccatc
     1561 gatgaatcct ggcactagat attgcccatt agcattatgt acgctttttc cttcgtattc
     1621 accgattcct acgatcattc cttcgcagat tgctacatct ttttccatga tttctttcgt
     1681 aaagacgttg acgatcttag catgcttgat cactaggtca gcaggctttt ttttacttgc
     1741 tacttcgata tatttcctta atgttgtttc caatggagtg cctcccaaga taaattttta
     1801 taagtataac ataaattcgg tttttgccaa tatttttttg taaattcatt ttatttgaaa
     1861 caaaaagatc aaaaagacgt atgttttttg tggaggggag aataatggaa ataaaacgat
     1921 tatcggatca aatgataagt gaaagttttg ccaaacatgc ccaagacgta tatatattgc
     1981 cagcttcaga agtattttac atctattttc gctatggaaa aaaacgaaca ttctggaaga
     2041 aagtctcttc ggcagatgga cagcaactga tctcaagatt taagtatttg ggacagatgg
     2101 atatcggaga aaaaagaaaa gctcagttag gtgcatttac ttattcttta gaaaaacagg
     2161 aagttcgtct gcggttgtct tctgtaggag attaccttca acgagagagt ttagtgattc
     2221 ggctattata cggtactttt gaaacacccc atcgttgctt tagttctcga gattttgcaa
     2281 ctattttaca gaaaaccgtc agacgtggat tatacctttt cagcggacca gtcggatcag
     2341 gcaagacttc tctaatgtat cggttggctt tggaagaaaa attgcaagtg attactatcg
     2401 aagatcctgt agagatagaa gaaccgtttt ttctgcagct acagatcaat ccgaaaatag
     2461 cacagactta tgatacactt ttgagactcg cattacggca tcgtccagac ttgctaatca
     2521 ttggcgagat aagagatgaa tcaactgcac atgctgcggt aagagcagca ctaacagggc
     2581 accgggtttt tgcaaccgta catgcacgca atcttgaagg tacattggtc agaatccatg
     2641 aactgacaaa gaaaaaagaa gagctatctg aatgtttagc tggtgtcatt tatcaagaac
     2701 ttttggaaga tgccaatcac catccttctg ttttatggag ttatcaattt atggaggaag
     2761 aatcagtaaa aaaatcatgg gaggaaagtt atgaagaagc tcaggaaaat atttggcagt
     2821 cagaactatt ctaaaggatt aacaagaagc cagcaaaacc agtttgcaca aaccatggga
     2881 gaactgcttt tatctggttt tagtctgcaa gaatcccttt atttattact cacgatccgt
     2941 tcgtttccac ctgttatttt ggcaaatatc caacatcagc taaaggaagg gcaagcgttt
     3001 agtgaggtac tttcgcaaat aggatatgga gcagagcaac tggtacagat cgagttagct
     3061 gaacgtcatg ggaatctgcc gacaactttg ttggaaattg ccaaacagtt tcggcttgtt
     3121 gaaggatttc gcaatgattt gaaaaagctg cttgcatatc cgatgctatt gctcaccttt
     3181 ttgctaggta tcttgtttag tatgcggttg ttcgttttgc ctcagcttat tggttcagga
     3241 atggtcatgc aagagcattg gggtattcag ctgatacaag tctttcattg gtatgttcta
     3301 ctattcatcc tagcggttac tctattggct ttactagtaa ggtatcgtct gatgaagttg
     3361 tctcctgttg atcagagtga atggataagc agacaattat tgattggacc gatatattcc
     3421 ttgtatcaat cggcttattt gtcactagag attggaaagc tttttcatga aggtttcgaa
     3481 ttaaggcaaa tcatagtctg cttaaaggaa acgaaacaag aaagtctgat tcagcatctt
     3541 gcctctaagt tgattcaagg attggaggca ggtatgtcat tggctgagca gttccagaat
     3601 tatcgttttc tatcagaaga attcagtaaa attgttttac agggagaggc aaagggaagt
     3661 ttaggtaaag aactgatttt ttacagcgaa tggacaagaa aacagctttt caaacgtctt
     3721 catcactgga tgcattttat tcagccaatc atttttttgg gcgtagcggc tttgatttta
     3781 ttagtttatg ccgctgtttt attacctgtt tacggaagca tagaggaggt tttgccatga
     3841 aaaagagaaa aatatcttat tatagcggtt ttacattgat cgaaatgcta atcgtcttac
     3901 tgatcatcag tgtattagtg ttgttgttcg tccctaattt gtcgcgttat aggaatcatg
     3961 tagaccaaga aagtcgtgaa gcgatcatcc agttggtaga cacacaaaaa gagctctatg
     4021 cgcttcaaaa taatggaaga gttcctactg tggaggaatt gttgaatgaa ggctatatca
     4081 aacgagaaca tgcagaaatc tatcagcggc cttgaaggta aggttttagc atcaggatat
     4141 acgttgtttg aatcactctt actgatttta ttacttacaa cgatcctttc ttttccagtt
     4201 ctcgcatttt ctgcatggaa gaatgaattg gcagtctatc aatttttcgg ccaatttgaa
     4261 aaacgaatct attctacgca aaaaatcgct attgtgaatc agatccaaac aggcttttat
     4321 tggaatagtg aagagaatca aatcattttt catgttccga atccagataa aataaattgg
     4381 gaagtattag atattcctga tgagattacc ttgaaacgcc atgcttctat tacttttgct
     4441 gctagaaccg ggaatgaaag ttcactaaaa gcttatcaat tttattggga agaaaaagag
     4501 cggacgatta cgtatcaatt ccagataggg agtggtcgct atacaaaaag aattgaataa
     4561 aacaggattt gttttgttgg agtcccttac agcgcttatg atcgcttctg tctgtgtcta
     4621 cttagtgagc tttggccagc tgtttttgct tcaggcagaa aagaaaagtg gggaggaact
     4681 gctatttatt cgaatgattt atgaagatat caagacgcag cggatgtatc acaaaagacc
     4741 agagcgaagg gaagaaatga tcccatacga tatccaaatt gtcgataata atggagtaat
     4801 cgaaaaagtg tttgtcaata tggagaaaga agcgatcagt attgaaaaaa caagtaattc
     4861 cagcatttac gctgattgaa tgtctaattg gactgatgat attgagcagt ctattgctca
     4921 cattcaatct tttgattcag caaaccatcc aagtaagtga atttttgcag cgtaaagatc
     4981 aaaaagagtg gctaatattt ttagcacaat tagaagaaga actcaagagc agtcacaatg
     5041 tctctgtgaa aaatcagcaa ctgcactaca cgatcgagga taaacagtat atcatcgaaa
     5101 gatatcaggc aatgatacgt aaaagaagaa cttccggcgg acatcaacct atgttaacaa
     5161 gtatcactga gcttcaatta ttggaaaaag aacacacaat ttctttttat gtacactttg
     5221 aaaatggaga agatggatat gccatatgga caaaaaacga tcagtagaaa atgttataag
     5281 ggctctattt tattttctgt cttgctttta tttcttttat gtacgttttt attgatggca
     5341 gcattagaaa gctaccgttt gtcaatggat cttaatatac gaatcaaaaa ttattatata
     5401 gcaaaaataa tgaagcagat ggctttatcg gaacagtcta cgcttgccga aagttcggaa
     5461 ggagtttttt actacacaac tggatctgta acatatcagt gggtacaaca gtccctattc
     5521 ttagaggtgg aagtctcacc gtttatcttt cggttcacag aagaagtaaa aaatgataca
     5581 gatactagta cagaatgatg gaatgttcgt gaaaacgccc tcttttctgt tataacactg
     5641 tttttaataa aaaaatgttt gccgtttcac aaataaaaaa cagtgaaaac gattgaaatt
     5701 ttgtcaattg ttcgatagaa ttaaacaggt gaaaaaattg ttataatata tctagaaatg
     5761 aggtgcacca tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt
     5821 cagcttttac agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa
     5881 aatttaatag atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg
     5941 caacggatca cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg
     6001 cgcagactgt ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac
     6061 catcaactca ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac
     6121 acaaataaaa aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta
     6181 ttaacagttc ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat
     6241 attgatgata cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt
     6301 caatactttc atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc
     6361 agcgatttgc caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt
     6421 accgaagaag gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg
     6481 aagccggatg gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt
     6541 gaagaaatca aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgcca
     6601 gatgaattgt tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga
     6661 ccacaagcac aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca
     6721 gagaaggtga cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca
     6781 ttcaacgaga aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt
     6841 ttgaaatggc aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa
     6901 cgtatcggtt tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa
     6961 caaatcatgg atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt
     7021 gaactgcaaa ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac
     7081 ggaggagaaa cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct
     7141 gaattagcag aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt
     7201 aaaaagattt tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca
     7261 atgccaaagc aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta
     7321 agaaaatatg gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta
     7381 ttgaacaaac cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg
     7441 atcacagcag tcgatggagg taaatccatg gatacttcta tgggattcac accattagct
     7501 ggggtgacaa tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg
     7561 gaaaaattag agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg
     7621 ctaggcctaa caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa
     7681 catattcgtt tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat
     7741 gtgacaacga tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac
     7801 ggacatgtac gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca
     7861 gcgttgaatg aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga
     7921 gtattagtga tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc
     7981 ggctaagcca aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc
     8041 atgtgttttt tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt
     8101 tttacaggat cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc
     8161 attttattta ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg
     8221 gacattacat atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt
     8281 attcttcgct ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa
     8341 aaacttgatt ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag
     8401 ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct
     8461 gctcctgatc aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc
     8521 tgactgctag gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat
     8581 agctgtcgag gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga
     8641 gtttcagcaa cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca
     8701 catcgctttg ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag
     8761 tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg
     8821 gcaaataacc gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct
     8881 caccgagtaa taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta
     8941 aatgatctcc acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact
     9001 gctggtgcaa gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc
     9061 tttgcagccg atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc
     9121 gttccagttc tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac
     9181 gaaaacgacg aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt
     9241 ttgtcagctc agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat
     9301 actcaatcaa tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt
     9361 gccgatcatg agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc
     9421 agcgaatact gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat
     9481 cggctgctaa aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta
     9541 accgtgcttc ctgacggtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt
     9601 ctaatacgta accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac
     9661 acttctctaa gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt
     9721 tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa
     9781 ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc
     9841 tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga
     9901 taccgactaa ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg
     9961 gattcccgcc gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg
    10021 agaccactca aaaaggccat ggttatccat ggcctgtgtt atttaaactc agattatcat
    10081 gagaaaaata ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact
    10141 tgtcccgtca acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg
    10201 gtaattttca ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt
    10261 agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact
    10321 taatatttat cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt
    10381 ctcgtggacc acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg
    10441 tttgataaaa aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt
    10501 aaacagctct tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg
    10561 caatggcaag cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc
    10621 ttctcgcggt acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc
    10681 atcgatactt catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa
    10741 taagctttgg catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt
    10801 tcactttttt ttattttact tgaactcatt gactgttttc cctttatttt aaacattata
    10861 aagaccgatg agtgcttgcc gcgtcttttg tttttatcac ggttttttgt gcaaaaaaac
    10921 ctgccgatag ttggcaggtc gtctacgtgt taagctttgg tgtggtcttt agctggcaat
    10981 tggttttcta aatcagttcg gacaacaagc acatcgcaag ctgcgtttct aataacatat
    11041 tcagaaacag aaccaataaa taaacgttct acagcattca atcctgtggc tcctaacata
    11101 atcaaatcta cttgattatc ttctgggatt tctctggcaa taatttgttt tggtgagcca
    11161 tattctacga cagaggttac attattcaag ccagctttct tcgcattgct ttcataatct
    11221 gccaatgttt gctttgccat ttcagtagct tgttctgcaa gcattccatc aaaggatgaa
    11281 acggtttgga aagaacgtgt gtcaatgaca tgtgctaata gaagttctcc gttatttcgg
    11341 atagctacat tgaccgcctt tttaaaagct aattcagctt catcagaacc atctacggca
    11401 accatgattt ttttgtattg ttgtaacata agcattcact cctcaagtca atcgtattgt
    11461 actcttttat tgtagaacac tttagggaaa aagagaatta tttactgctt tggttcagca
    11521 attttttttg caaatcggat actgaaggtg gcagaaacaa taattacgat cagtaaaaag
    11581 accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa tacccattac gcctaaaaca
    11641 gcatatgcag ctccgtaaaa ttgtaggaat ccttgatttt tatcattttt gttgatcaat
    11701 acaaaaaaga tatcctgttt attaaataaa taatagctaa cgaataataa tactaaagtg
    11761 gcgataatca ttaaaatcct aatcatgaac aaatcctcca aaataagcaa caaatagaaa
    11821 aagcaatttg ccaagagcaa attgctttat tctttaaaag atggtaatct ccgttaatgc
    11881 cgttgtttgt cctgtagtat tgatcccgcg aattcaagca ggtgggtccc atggtcgtag
    11941 cttcgaacta ccaaatgata aggcatgtta ccagcagtga cacaagacgg gtcccaagat
    12001 atcaataaaa atgttcggtc aacacacaaa gaggacaatt cgaacatcac agatttccca
    12061 cctctatagt agcatatgaa tttaaaaaga gcaatgagag agctcgcaga aacagaaccg
    12121 ttttcttgtc tagttttttg gaggatgctt tttccactct tttatacggt aatactgttg
    12181 acccagagcc tgttcatatt tccctgtatc ttttggctga taatactgag cattcttcag
    12241 tttatctggt aaatattgct gattgaccca agcctgatca aaatgatgag gatactgata
    12301 acccacgcca cggttgagtg atttcgcacc tttgtaatgg ctatctctca aatgatcagg
    12361 aacatctcca gctttgccct ctcgaatatc agcaagtgct gcatctaatg ccatgtaggc
    12421 agaattggat tttggcgaca agcaaagatc gacgacgacg tctgctagtg ggatacgtgc
    12481 ctcaggcaat cccaattttt cagcagctaa tacagcgttc actgtacgag ccgcagcagc
    12541 aggattacct aaacctatat cttcataacc gataaccatc agacgccggc aaatacttgc
    12601 cagatcacct gcctcaacta aacgggcgag ataatgaagt gcagcatcca catcacttcc
    12661 acgaatcgat ttttgaaaag ccgaaataac gtcataatga gcatctccat ttttgtcatg
    12721 ggtaagagct tttctttgga cacattcttc aatgatcgac agtgtaagat gaatccttcc
    12781 ttctttatct gaaggagtcg atagtgtagc cagttccagc ccattcaatg cactccgcaa
    12841 atcgccgttc gttgcacgcg aaaggtgaag aagagcatct tcgtctaact ggattgcttg
    12901 ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg actgccaact ggatgtcttg
    12961 ttctgttaat ggtttgactt caaagatctg tgtacggctg cggatcgctg gattgatggt
    13021 aatataagga ttctcagtag tcgctccgat caagatgatt cggccacttt ctaaatgagg
    13081 aagcagaaaa tcctgttttg ttttatctaa tcggtgtact tcatccaata agaggatcac
    13141 tgtaccactc atttttgctt cttccgctac aacttgtaga tccttttttg aatctgtagc
    13201 ggcgtttagc atgcgaaatg cataattagt agagccggcg atcgcacttg cgatactcgt
    13261 ttttccagta ccaggaggtc catatagaat catggaagag agcatgcgag catcgaccat
    13321 gcggcgtatg atttttcctt ccccgactag atgctgctga ccaacgactt catcgatcgt
    13381 tcgaggtctc atgcgataag ccaatggctg ttgcatagga ttttcctcct tcttgttttt
    13441 acgtccctta attatatcat atttagcaag atgcctagaa agaacagcct tctgttgttt
    13501 tgttttcata gcttatccaa ctaatattgt gtatgataaa gtaggtgaga aaaatgacgg
    13561 ataaacaaaa gatagatgaa tttttttgga ataaaacgac gccagaagtg gcacgggatt
    13621 tattggggat gtatttggag catgacacac cagaaggaag attagctgga tatattgtag
    13681 acgcggaagc atacctaggt ccagaagacg aggccgccca tagctatggt ttaagaagaa
    13741 caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat ctacctttac acgatgcata
    13801 ctcatcggat tttgaacatc attactcagc cagaagggat tcctcaagga gtaatgatac
    13861 gtgcaatcga acccgccgca atgattgatc aaatgagtaa aaatcgtggc ggaaaaactg
    13921 gtccagatat cagcaacggt ccgggaaaat tagtggaagc attagcaatt cctcaagaac
    13981 tttatggtca gtctatagca gatagttcgt tacgcttagt ttttgagaaa aaaaagacac
    14041 caaaaaagat caatgctttg cctagaatcg gtatcccaaa caaaggtgta tggacagaaa
    14101 agccgttgcg ttttgttgtg tcgggaaatc cttatatctc tttacaaaga aaaaaccaaa
    14161 tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga aggatctatt aacatattta
    14221 gatgaaataa ttgaaaaaca agtgacagac tatgatgtag ctttagattg ggatacaaaa
    14281 aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca atttgagatt
    14341 gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga cggcgtactt
    14401 ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt atctagcagt aatcccttat
    14461 gaaggaaaaa aagggttggc aaaatcagta gctgattctt tagtggaata tctaaatgaa
    14521 gtattagctc aagggcaaag cgatttgctt gattttttgg atgaagacga tgacgaagct
    14581 gtattcgagc ttcactggga taatcagcaa ctagcagaat tagtagaagt aaaacagcaa
    14641 aatgaagcag atacttattt accgtaccca agttattaag gagtgacaga tatgaaatgg
    14701 aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa tattttgatg
    14761 gaggcagggg cgagtggtgt cgcgattgaa gattctttag atgttgaaca ttttcagtcg
    14821 gatcaatatg gtgagatttt agataaaaat gattttacat cgctgaaaga aggcgcacta
    14881 gtcatggctt attttccgga aacgactttc ttaccggaaa tcttgccttt tatcaaagat
    14941 cgtatcacac aattacctga gtttggtcta gctattggaa aaaataaagt aacagttagt
    15001 gaagtagagg aaagcaactg ggcaacagcc tggaagaaat actatcatcc ggtgcgtatt
    15061 acgcgaatgc tgacggttgc gccaagctgg gaaaactacc agacagaaga tcctctagaa
    15121 aaaattatca cactagatcc tggtatggca tttggcacag gcactcatcc cacgacttcc
    15181 ttgacattga cagcactaga aacagtcttg cgtggtggtg aaacattgct agatgtggga
    15241 actgggtcgg gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga tgtctatgcc
    15301 tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa atatggatat gaatgaaata
    15361 gccaaagatg tccatgtatc agctaacgat cttttgacag gaatcgagat tgaaagtgat
    15421 gtaatcgttg cgaacatttt agcagatatc atcttattga tgatcccaga tgcttggcgt
    15481 ttactgaagc aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa gaagcaaatg
    15541 gttctggatg ccatgttaga acaagggttt atcgtggatc aaattttcca gcaaaaagac
    15601 tggtatgcaa ttattttgaa aaaaccagag gtggaataga tgcagcggta ttttttaaaa
    15661 gaatcatatg aatcaaaaga ctattttgtt ttagaagggg aaaactatca ccacattgta
    15721 cgggttatga ggatggaacc cgaggatgaa gtttttttgg cattcaatga tcaaatagcc
    15781 attgttgcag aaatcacgga gattactgaa gaggctgttt atctgaaaga aattaaaaaa
    15841 gaagaaaatc aaaaggaact gccaagttcg attaccatag cgagtggcta tcctaaaggg
    15901 gaaaagctgg attggatcgt tcagaaatcc actgaattag gagcgcaagc ttttcttggt
    15961 ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa acgacaacaa
    16021 cggcttgaaa agattgctca agaggcagcg gaacaatccc atcggcagat cgtgccatca
    16081 gtagagttat tggaaacgaa acaagattta tttgagcggc tagatgatta tgacgtggtc
    16141 ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc caaaattctt
    16201 agtgaaacaa aaccagatag tcgaatctta gctgtctttg gaccagaagg tggattatcc
    16261 cgcgatgaag tcgattattt tgtagaacac aaagggatac tttgtggatt aggaccaaga
    16321 atccttcgta cagaaacagc acctctttat ttattaagtg ctgctagcta tcaatgggaa
    16381 ttaatgaatc cttctttcag ctaacgttct tcattaataa aagagtatcg acagaaagaa
    16441 ttgaaaaaat agtcaacaat ttgtataata gaaacaatta tttatacaat ggatacgaaa
    16501 aatcgtttga aaaagctttt aaaaagcgaa catttccgta aaaaaacgta taactgcacg
    16561 gattttggga caattatcaa acagatattt gtcccgattc ccgttttacc gaaaaaagaa
    16621 agggggatcg attcctgtga caaaagaaga aattttaacg ggacccggtg tcataaagct
    16681 cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc
    16741 gactgctgca catgacggac agtttagaaa atcaggcgaa ccctacatta ttcatcctat
    16801 ccaagtcgca ggtatattag cagatttaaa aatggatccc catacagtgg ctacaggctt
    16861 cttacatgat gttgttgaag atacagaaat cactttagaa gatctgagag aagaatttgg
    16921 cgatgacgtt gctatgttag tggacggcgt aaccaaatta gggaaaataa aatataaatc
    16981 ccacgaagag cagctagcag aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt
    17041 acgagttatc atggtcaaat tagctgatag actacataac atgcgtacgt tgaagcacct
    17101 gcgagaagat aagcaaagaa ggatcgctca ggaaacttta gaaatctatg cgcctcttgc
    17161 acatcgtcta gggatcagcc ggatcaaatg ggaattagaa gatacagcac ttcgttatct
    17221 aaatccaaaa cagtattacc gtatcgtcca cttgatgcag acgaagagag aagaacgtga
    17281 aaaatacgta agtggtactg ttgaagatat tcgaatagcg acagaagagc tggggatttt
    17341 tgcagaaatc tatggacggc caaaacacat ttattcaatc tatcgtaaaa tgaaagatca
    17401 gaaaaagcag ttcaacgaaa tttatgactt gctagctatt cgagtgatcg tagattcaat
    17461 caaggattgt tatgctgtat taggagcaat ccacacaaaa tggaaaccaa tgcccggcag
    17521 atttaaggat tatattgcca tgccaaaagc taatatgtac caatctttgc atactaccgt
    17581 gatcggtccg gcaggaaatc cggtagaaat tcaaatccga acacaggaaa tgcatgaaat
    17641 cgctgaattc ggggttgctg cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga
    17701 accagatggt atgacgaaac aattaagctg gttccatgag atactcgaac ttcaagacga
    17761 aagctatgat gcttctgaat ttatggaagg cgtaaaagga gatatcttta gtgataaagt
    17821 ctacgtcttc acaccaaaag gagacgttac tgagttacca aaaggatccg gaccattgga
    17881 ctttgcatac agtatccata cagatatcgg taacaaaacc actggtgcaa aagtaaatgg
    17941 caaaatggtg cagcttgatt acaaattgaa aaacggagat atcattgaga tcatgacttc
    18001 tccaaattca tttggcccaa gtcgcgactg gttgaaatta gttgctacta gcaaagcaag
    18061 aaataagatc aaacgtttct tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca
    18121 cgaatccgtg gtcaaatgta ttacagatct aggatttacg cctaaagata ttttgacgaa
    18181 gaacaaactg caagaagcac tcgatcgttt taattatcaa acagaagatg atctctatgc
    18241 agctgtaggg tatggagaag ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga
    18301 acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc
    18361 gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt
    18421 agaaaactta ctaattcgta tcagtcgctg ctgtaatccg attcctggtg atgatatcgt
    18481 tggttatatc actaaaggca gagggatatc cattcatcgt cgagattgtc cgaatgttca
    18541 gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc gaatgggaag atacatcgaa
    18601 tacacgaaaa gagtatgatg cagatttgga aatttacggc tataatcgtt caggcttatt
    18661 gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg
    18721 gacgaataaa gataaaatgg cgacgatcca tttgacggtt ggtatccaga atttatccca
    18781 cctaaagagt atcgtggata aaatcaaagc agtacctgat gtctacagtg tacgccggac
    18841 gaatggatag gaggacagat atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt
    18901 cagtcgatca gcaagaaatt ggaaaaattg gctacggcct acttgtttta ttaggcattc
    18961 atgaaaccga tacgcaaaaa gatgtggact atctggtaaa aaaaattgca caaatgcgta
    19021 tttttgaaga tgaacaagga aaaatgaata ttagtgtaga agatgtggga ggacaaattt
    19081 taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg
    19141 tggaagccgc acgaccagaa acagctattc ctttatacga atcattcaat gaaggaatca
    19201 gaagtcgcgg catcacagtc gctaccggac aattcggagc agatatgtcg gtttctctcg
    19261 tcaatgatgg acctgtaacg atcattattg attctaaaaa caaataaagt aatctggttt
    19321 tcactagtaa aaactatgaa acacgtcgct taagttagac tgataaactc taacttaagt
    19381 ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta
    19441 aattatacaa gttatttttt ataggaggtt gctgaagatg aaagaagtac cagtagtaga
    19501 tatcaaaatc acagatttaa aaaaattgta tcaaaaagga tacaacatgt tggaggagtt
    19561 acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac
    19621 gatctacgga tcatcagcag caaaagtatt ttatgatcca aggaatttca aacgaaaagg
    19681 tgctatgcca aaattagtat tgaagacact ttttggtcaa ggtggagtac agactttgga
    19741 tggagctgct catcaccatc ggaaaaatat ttttatggat ctaatgacac cggaaagaat
    19801 ggaagattat catcgtatct tagataagaa tctgacacaa gcacttgaag cacagcatgg
    19861 tcaatttgaa ctatttgatt taagtaagat ggttttcttt acatctattt gcgagtgggc
    19921 aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa aaattagcag aatatcaaat
    19981 ctctatgatc agcggaacgt ttacttctcc gattgatcac ataaaaggtg tcgaaaatcg
    20041 caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc
    20101 tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc acagatttag atggtcagct
    20161 tttacctctg gaagtggcag ctgttgaatt attgaacatc atccgtccaa cggttgctct
    20221 aactgtgtgg gcggcattga tgggacatgc acttttcagt agaccagatt tgtatcagca
    20281 attaaaaaat gacttttcga cgttacaaga tccgtttatt caagaaatga gacgctatta
    20341 tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag atggttatcg
    20401 tataccagaa ggcagttggg ttattttaga tttatatgga acagaccatg atgaacgaac
    20461 agttgaagcg ccagattcct ttatgatcaa acggtatgta gggaaagcaa aagatatttc
    20521 ctataaagaa gaatatgaga tgattgcaca aggcggggga aattttcgac aaatgcatcg
    20581 ctgtgcagga gaatggatca ctcttcatag tctacgtgtg ttttccgatc agctagtaaa
    20641 taagtttgag ttctctgtac ctgaacaaga ttggacgatt ccattcaatc agtttccgac
    20701 ttatccgaat agccgagcgc tactttacaa gaattagatt agcataaact tatcgaaaaa
    20761 aaggaagatc aaacagtgct gatcttcctt ttttcatatt actataaatt gataaggtca
    20821 attgatcaaa atccacgaaa tgcttcatca atggtttgat aatcttcatt ggataaatgc
    20881 aaatctaaag cttggacatt gtcagatact tgctctggac gcttagctcc aggtataact
    20941 acagacactc gtggattctt catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg
    21001 tatcttttag caatggggcg aagtacatcg acagcggtta aaatttctct gaaacgttct
    21061 ccttggaaat ctggatttct ttttcgagga tctccttcac caaattgttt ttcctcacca
    21121 agttcatatt ttcctgtcag caaaccggaa gcaagaggaa aatagggaac aaaactgatt
    21181 ttgttttttt caagataagg gaataattct ttttcagcct gccgatggat caaactgtat
    21241 ttatcttcta ctacatcgac atatccatct gcattcgctt ctttcaattg ttctaacgtg
    21301 aagtttgaca caccgactgc tcggattttt cctgcttctt ttaattcgtg caatgtagca
    21361 actgattcat tttttggtgt gctttcatcc ggaaagtgga tataaaaaat gtcgatgtaa
    21421 tcagtttgca gacgttttag tgcatcttcc accgattgtt tgagaaactc aggagaatta
    21481 tcaaatgttc gccctttatt tggtacatga gcagcttttg tagcgatgac gacacgtgag
    21541 cgatcatatt ctttttcctt cagtacctcg ccaatcagtt cttcggaacg gccgtttccg
    21601 taagcataag cggtatcgat caaattgatc ccgctattca aagctgtacg aactacttgt
    21661 tttcctgtct catcttccaa accgtcaaaa agattatgtc ctccaacggc atttgctcct
    21721 aatccaagct gttctgcgta gacttgtgaa tgtccgattc ttatttctgc catagctgat
    21781 tcctcctttt tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg
    21841 aggggaaata gtcagatatc tcttgacttt gagactggtt tttagtaggt tataagtaat
    21901 atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt tacaagagag gatcatcgag
    21961 gctgaaaatg atctaaagtt tcattagcct aaagacactc tggagatgga cgagtgaaaa
    22021 gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga aatctttcaa aattaggtgg
    22081 taccgtgtat gaatttcatt cacccttgtt catgatgaac aagggtgatt tttttattta
    22141 catagctaag aaagggagag aaaactatga gtttccaaag accaaaagga acaaatgata
    22201 ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt ttattattca
    22261 gagattatca gtataatgaa atgcgtacac cgatttttga acactatgaa gtgatatccc
    22321 gaagtgtagg ggatacaacg gatatcgttt caaaagagat gtatgatttt tatgacaaag
    22381 gggaacgcca tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt tcttttgttg
    22441 agcataaatt gttcggccca gagtatgcga aaccgttcaa aactttttat atgggaccaa
    22501 tgtttcgcta cgaacgccct caaaaaggac ggttacgtca attccatcaa atcggagtag
    22561 aagcatttgg atcagaaaac cctgcaactg atgtggagag catggtgatg gcacttgatt
    22621 tcttcaaaca gttggggatc acgcaaatcc ggctagtcat caattcatta ggtgataaag
    22681 aaacgcgtca agcctatcgt caagcattga tcgactatct tttacctttt gaatcagagc
    22741 tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt acgcgtattg gacagtaaag
    22801 acaaacgaga tcaaaaattt gtggcagaag ctccatctat cttagattat ctaagcgagc
    22861 ctgctcaaaa acattttgat accgttgttt ccatgctgga tgcattggct gttccttatg
    22921 aaatcgacag taatatggtt cgcggactag attattacac acatacgatt tttgaaatca
    22981 tgagtgacgc accaaaaatg ggggcacaag caacgatttg cgcaggcgga cgttatgaca
    23041 atctagtgga agaactaggt ggtccttcaa cacctggctt cggatttgca atgggtatcg
    23101 agcgattact cttgactatg gaggcagagg aagtagttat ccctgcattt aatgagttgg
    23161 atgcttacgt ggtcgcactc ggagacgaaa caaatatcga agcattgaaa gtggtccaag
    23221 cgattcgaaa ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc
    23281 aatttaagac agcagataaa gcgaatgcca aattagtatt ggtgatcggt gaagatgagc
    23341 ttgctagtca aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat
    23401 tagaagagat atatgaacat tttgatgaag tctatgatga aatgacttta ttcgagtagg
    23461 aggaaaaaaa taaatggcac aaagaacgat ctattgcgga ttagtttccg aacaacaatt
    23521 agaacaaaca gttacattaa aaggatgggt acaaaaaaga cgagaccttg ggggagtaat
    23581 ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa
    23641 caaagaagca tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg
    23701 ggaagttaga agacgcggag aaaatgcagt aaatcctaaa atggccactg gtgaattcga
    23761 agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa accccgccat ttcaaattga
    23821 agatgatcaa gcaatcagtg acgaaatacg tttgaaatat cgttatttgg atctgcgtcg
    23881 tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta
    23941 tttagatgat aatgactttc tcgatatcga aacaccttac ctagggaaat ctacaccaga
    24001 aggcgcaaga gactacctag ttccttcacg tgtccatgct ggacatttct atgcgttgcc
    24061 gcaatctcct caattgttta aacaattgct gatgaatgct ggattcgacc gttattatca
    24121 aatcgttcgt tgtttccgcg atgaagattt acgtggagac cgtcagcctg aatttacgca
    24181 agtagatatt gaaacaacgt tcttaacagc agaagaaatc caaacttaca cagagggatt
    24241 gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc acattgccat ttcctcgtat
    24301 gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac gttttgatat
    24361 ggaattgatc gatctaagcg atacagtcaa agaagtagaa ttcaaagtct tccaaatggc
    24421 tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc
    24481 acgaaaagac atggatcagt taggtcaata tgtaggacaa ttcggagcta aggggcttgc
    24541 atggctgaaa gtagaagaag acggattgaa aggtccaatt gctaaattca tgggagaagc
    24601 aacagaagca atcattaaag caacagatgc aaaaccaggt gacttgctga tgtttggcgc
    24661 agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt taggaaaaga
    24721 attaggacta attgatgaat cgaaattcaa tttcttatgg gtaactgatt ggccacaatt
    24781 tgaattttca gaagaagaag gtcgatatgt ctctgctcac catccattca cgatgccaaa
    24841 agaagaagat atcccgttat tggctgaaga ccctgcaaaa gtctatgcag aagcttatga
    24901 tatcgtcttg aacggttatg aactaggcgg aggttctatc cgtattcata ctcgtgagct
    24961 tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa caaatggaag aacaatttgg
    25021 tttcttgtta acagctttag attacggatt ccctccacat ggcggtattg cattaggatt
    25081 ggaccgtttt gcaatgttat tagccggaga agataatatt cgtgaagtca ttgcctttcc
    25141 taaaaacgga aaagcagcag atgtgatgag tgatgcacca agtatggttt cgcctttaca
    25201 attgttcgaa ttgaatattg atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa
    25261 aggatttgtc gaatacgctt cttttgacaa ccttgattta attgaaaaac actttataat
    25321 gaagccataa catataaagc ggggcttttc catgaaaaaa acattcgaaa attgggctta
    25381 tcatgattac acgaccaagc tttcggtatc tatcgtttat gcgttgatcg catcagtcgc
    25441 attgaatttc ttttatcagc caggacatat ttattcaagt ggtattactg gtctagctca
    25501 gattttgacg actttgtcaa aatcactgat aggattcgaa gtaccggtat caacaacttt
    25561 gtatttactg aatatacctt tgtttgtatt ggcgtggtta aaaatcggga agaagtttac
    25621 aattttcact tttatcacag tcactttgac ctccctttgt atccatattt taccggaaac
    25681 agtcctatct accgatccaa tcatctgtgc gttgtttggg ggggctgtga tgggactagg
    25741 tattgggcta gcgttgagaa acggcctatc atcgggcggt ctagatatcg tgagtatcac
    25801 gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg atctacttca atgcattgat
    25861 cgtttttgtt gccggaattc tatttggctg gcaatatatg ttttacagta cgttgtctat
    25921 tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat
    25981 gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg
    26041 gatcacgatc atcaatgaag cagaaggtgc ttataagcat gataagcaga cagtattgat
    26101 gacgatcgtt acacgatacg aactgcctct acttagagaa gcaatgaaag cttctgatcc
    26161 acaagctttt gtaagtattg cagaaaacgt ccagatactt ggtcgtttct atgaggatgg
    26221 catttagatc gagtttgaca aaaattcatg aaatactgct gtagacagac gttcgtttgt
    26281 ctgcagtttt tttctaccat agtagaatca gcaaagaaaa actgcttttt tatttgaaat
    26341 tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat ttatgggatg atagaaaaaa
    26401 atagtagaga aaggacacga ttctacttgc tattttcact taatacccgt atattacgaa
    26461 tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca
    26521 gccgctacat cactgggttt gatgggctcc gttcattagc tgtggtgggt gttatcttct
    26581 atcaccttct gccaactagt atgaaaggcg gctatttggg cgttccgatt tttttcgtcg
    26641 tatctggtta tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca
    26701 atatttggca attttatgtc agaagaatga agagactcta tccggcactt gtttttatgc
    26761 ttgtgacagc ttcggcttat atcactttgt ttcagcgagg gctgttaaac aatcttcgag
    26821 gagttgtggt cagcagctta ctgtatgtca acaactggtg gcaaatcaat aacggtttat
    26881 cttatttcga tcgatttgct aatgagtcac cgtttacgca tatctggtct ttagcagtag
    26941 aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt gttgatggta tttgttcgta
    27001 aaagaaaatg gatcttttat actgtgttag gtacatcctt cctctccgcc atcttgatga
    27061 tgatcttgta tacaccagga ggcgatccaa cccgtgttta ttacggtacc gacactcgtc
    27121 tgttttctat atggttggga agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga
    27181 aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg
    27241 cactgattct ttgcttcttc tttttagatg atcattacaa tttcatctat tacggaggaa
    27301 tgcttctagt gagtctgtta tgcgtagtgc tagttgcagt gacagcacac cccggagcaa
    27361 gtttgaatcg atggctgaca aatccagtat ttacttggat tggcaaacga agctatggca
    27421 tttatctgta tcagtttcca gttatgattt tttatgaagc aaaggtgact gatattgccg
    27481 atcatgtttt gttgcatact ttagtggaaa tcacattgat tctaatcatc agtgaacttt
    27541 cttatcgata tgtggagcgt ccgctagcac gttttgatta tggtcgtaca ttccaagtga
    27601 tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa accatgggtt gttccaggca
    27661 cattacttgt agcaattgca ctcgttggtt tcgttacggc accgaaaaac gctgtaacag
    27721 ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca
    27781 gaaacaattc agaaaacacc actagttcgg ttgaccaagc gatccttgat aagtacgagt
    27841 tgtccaaggc acaagggaat aaggcacaaa aattagaact aacggcattt ggtgattcag
    27901 ttatgttgga tgctgcaacg gatctacaag aactttatcc aaaagtagtg gtagatggag
    27961 atgtcggacg acaattatat gccagtgtac cgtacattga aaaattaaag gaacaagatt
    28021 tgttgaaaga cactgttctg atcggactag gaacgaatgg ctcattctcg gaagctcagt
    28081 ttgatagtgt gatggatgca atcggtgatc gaaaagtata ctgggtcaat gtccgagtac
    28141 cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga acagatgacg aaaaaatacg
    28201 acaatatgac gctgattgat tggtacagct acagtaatga ccatagtgac tggttttatg
    28261 atgatcaagt ccatccaaat cctgaaggga tgacacatta cattcactta gtcagcgaag
    28321 ccttgctagg aggaagttca agtacgaaca gcagtgagac aaacgtgaca gaatgatagc
    28381 tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat atttaccgtg
    28441 tttttaacat tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt
    28501 ctttttgttt tcgggtgagt tttttgaatg cagcttctgc ttttgttgct tcgcttcttg
    28561 aagaaaatac ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt
    28621 tagcaccagt accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac
    28681 cataaaatgt ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc
    28741 atctcacgaa tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg
    28801 actttgaatc cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct
    28861 tttccaggtt ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg
    28921 acctgaatga tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt
    28981 tttagtagct tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg
    29041 gcgattgcca ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt
    29101 ggattacaga cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga
    29161 tcgatggtgt ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc
    29221 atgtctgcta gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt
    29281 aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt
    29341 acacgggaaa agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt
    29401 tggataatct tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg
    29461 gattccctcc aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac
    29521 caaggtggct tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga
    29581 agagactatg ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa
    29641 tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt
    29701 tgctccccat cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga
    29761 attcagcttt atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac
    29821 tcgatccaat gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc
    29881 tgtaaagatt ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca
    29941 tgctacagaa cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt
    30001 gccagctgta tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac
    30061 agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat
    30121 ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc
    30181 tgatttcaaa tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt
    30241 tgaatctact gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag
    30301 tctgccctct ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca
    30361 aagtgttata ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca
    30421 gccgattggc atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat
    30481 gtactatctt ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc
    30541 ggagacttat atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg
    30601 atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat
    30661 agcagtacga gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga
    30721 acacagctag cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt
    30781 ccttactttg aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc
    30841 aagcaagcgg tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa
    30901 caagtattga ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa
    30961 atcgaagtag gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta
    31021 gcactaggac atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt
    31081 tccccattga aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac
    31141 ctgacaacag gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa
    31201 ataacagcga gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag
    31261 aattacatct atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt
    31321 cggcaaatat atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca
    31381 aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt
    31441 agtgaaatga cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg
    31501 caagaaatca tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat
    31561 tttggtcctt ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt
    31621 ttgatcagcg ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca
    31681 ctttttggtg aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct
    31741 acaccagacg aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa
    31801 atcgaacgaa aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag
    31861 gcagccaaag taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca
    31921 aaacgtacag aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt
    31981 tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt
    32041 gataaagaga ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag
    32101 cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg
    32161 catcttctgc aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa
    32221 gccgttttag cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa
    32281 aaagaagctc taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt
    32341 tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa
    32401 ttagctgctc aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt
    32461 acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa
    32521 ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa
    32581 gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct
    32641 caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa
    32701 caagacgcag aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg
    32761 tatcaaaatc tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat
    32821 caggaaaaac tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag
    32881 cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa
    32941 gaactcaaaa aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca
    33001 gaacaagcag aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt
    33061 tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa
    33121 ttaaaacacg aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa
    33181 gctgagtctg ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat
    33241 aatcttcaac aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg
    33301 gctgaacaag aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa
    33361 caaaaaacaa aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag
    33421 atggccaatg caagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa
    33481 caattagctg tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa
    33541 ggagaaatca aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag
    33601 gagatacgac gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag
    33661 aaagcaggag tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa
    33721 catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa
    33781 aagttagaaa gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag
    33841 caagcacaat ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg
    33901 acagctgacc ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca
    33961 gaagagatca gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag
    34021 atatacggtt tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg
    34081 atttatcaca caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa
    34141 acattcaacg gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat
    34201 cttcaagaag tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat
    34261 caatttgagc tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat
    34321 atctatgatg atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt
    34381 tttattgcag cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt
    34441 ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct
    34501 ttggaaatgg caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc
    34561 attagtcatg taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa
    34621 ggtagcggac aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg
    34681 aaatggagta ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga
    34741 gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt
    34801 gaattgtata tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc
    34861 tattgggagg aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg
    34921 aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca
    34981 agtgagggag aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa
    35041 gcggtgcaac ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac
    35101 ccagagcttt ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa
    35161 acatatatcg taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac
    35221 actgttaata aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa
    35281 aaaatactca cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc
    35341 ttacaagtca aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc
    35401 ttcctgttag agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac
    35461 gatcaaggaa tacaattttc cgatcaaaac aaacctttat cattcaaagt ggaaaaattt
    35521 ggagaagatt atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag
    35581 tggggagtta tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa
    35641 acttggaaac aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa
    35701 ttaccgtctt tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt
    35761 gcccctgcag tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga
    35821 aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct atggcgaagt catctattcg
    35881 actgacccga agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta
    35941 gaagagaaac gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt
    36001 tatgaacgtc cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct
    36061 gtcttccgtc agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat
    36121 gcgcataaac atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga
    36181 ttcgatgtga cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga
    36241 aatgatgctt tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt
    36301 caacaaacaa gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg
    36361 atccatgtac ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct
    36421 acttttagtg aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag
    36481 gcacagcttc ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga
    36541 tggttgaaga tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt
    36601 gggaaaacac tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc
    36661 tctgctttga tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa
    36721 ttcgcccctt ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta
    36781 ctggctaaag acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct
    36841 aattatcagt cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat
    36901 agcagtacga aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg
    36961 agtgggacac caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg
    37021 ccaggatttt tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa
    37081 atgattcgac cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa
    37141 aaaacagaga tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct
    37201 tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac
    37261 cggatcggga tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg
    37321 tttattgaag attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta
    37381 gctgccaaag agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact
    37441 atcttgcaag aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg
    37501 aaaccacaag atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt
    37561 ctgatttcct taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc
    37621 ttgtatgacc tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg
    37681 atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa
    37741 cgcatggatt cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat
    37801 gaagaacagt tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa
    37861 atcgaggagt aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga
    37921 agcgattgtt tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc
    37981 ttccttgcta tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg
    38041 tctctaacag ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt
    38101 tgcgttctcg atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat
    38161 cattgtcgta attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct
    38221 cagctagctg aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca
    38281 tattgcggga aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt
    38341 cagtcggccc atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg
    38401 tttttttgac ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa
    38461 ataagaggcc atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga
    38521 tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt
    38581 catcactatg atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg
    38641 gaacgactct tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa
    38701 catcaaacac aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt
    38761 cacaaatcct tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt
    38821 tttcttacta tatttctatt tccttgacag aataaggttt ttatatttca gtttttttca
    38881 ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa ttaatcatta tggtagaagt
    38941 cgattgattt attt
//
LOCUS       DACDOA010000014        38148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDOA010000014 DACDOA010000000
VERSION     DACDOA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.014"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            171..1949
                     /gene="uvrC"
                     /locus_tag="IWI39_000657"
     CDS             171..1949
                     /gene="uvrC"
                     /locus_tag="IWI39_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9207784.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            2094..2993
                     /locus_tag="IWI39_000658"
     CDS             2094..2993
                     /locus_tag="IWI39_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207785.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            2986..4248
                     /locus_tag="IWI39_000659"
     CDS             2986..4248
                     /locus_tag="IWI39_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207786.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            4409..6307
                     /locus_tag="IWI39_000660"
     CDS             4409..6307
                     /locus_tag="IWI39_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9207787.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(6637..7356)
                     /locus_tag="IWI39_000661"
     CDS             complement(6637..7356)
                     /locus_tag="IWI39_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9207788.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            7693..8025
                     /locus_tag="IWI39_000662"
     CDS             7693..8025
                     /locus_tag="IWI39_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207789.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            8012..10015
                     /locus_tag="IWI39_000663"
     CDS             8012..10015
                     /locus_tag="IWI39_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9207790.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            10034..10504
                     /locus_tag="IWI39_000664"
     CDS             10034..10504
                     /locus_tag="IWI39_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9207791.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            10525..11109
                     /locus_tag="IWI39_000665"
     CDS             10525..11109
                     /locus_tag="IWI39_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9207792.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            11124..12110
                     /locus_tag="IWI39_000666"
     CDS             11124..12110
                     /locus_tag="IWI39_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9207793.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            12100..12411
                     /locus_tag="IWI39_000667"
     CDS             12100..12411
                     /locus_tag="IWI39_000667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9207794.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            12433..14214
                     /locus_tag="IWI39_000668"
     CDS             12433..14214
                     /locus_tag="IWI39_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9207795.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            14207..15583
                     /locus_tag="IWI39_000669"
     CDS             14207..15583
                     /locus_tag="IWI39_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9207796.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            15590..16225
                     /locus_tag="IWI39_000670"
     CDS             15590..16225
                     /locus_tag="IWI39_000670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9207797.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            16255..17610
                     /locus_tag="IWI39_000671"
     CDS             16255..17610
                     /locus_tag="IWI39_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9207798.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(17802..19196)
                     /locus_tag="IWI39_000672"
     CDS             complement(17802..19196)
                     /locus_tag="IWI39_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9207799.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            19288..20259
                     /locus_tag="IWI39_000673"
     CDS             19288..20259
                     /locus_tag="IWI39_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9207800.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            20256..21038
                     /locus_tag="IWI39_000674"
     CDS             20256..21038
                     /locus_tag="IWI39_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9207801.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(21207..21578)
                     /locus_tag="IWI39_000675"
     CDS             complement(21207..21578)
                     /locus_tag="IWI39_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9207802.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            21712..22155
                     /locus_tag="IWI39_000676"
     CDS             21712..22155
                     /locus_tag="IWI39_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9207803.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(22198..23007)
                     /gene="yidA"
                     /locus_tag="IWI39_000677"
     CDS             complement(22198..23007)
                     /gene="yidA"
                     /locus_tag="IWI39_000677"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9207804.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(23026..24393)
                     /locus_tag="IWI39_000678"
     CDS             complement(23026..24393)
                     /locus_tag="IWI39_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9207805.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(24377..25207)
                     /locus_tag="IWI39_000679"
     CDS             complement(24377..25207)
                     /locus_tag="IWI39_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9207806.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            25370..25807
                     /locus_tag="IWI39_000680"
     CDS             25370..25807
                     /locus_tag="IWI39_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9207807.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            25883..26503
                     /locus_tag="IWI39_000681"
     CDS             25883..26503
                     /locus_tag="IWI39_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9207808.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            26692..27198
                     /locus_tag="IWI39_000682"
     CDS             26692..27198
                     /locus_tag="IWI39_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9207809.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            27215..28114
                     /locus_tag="IWI39_000683"
     CDS             27215..28114
                     /locus_tag="IWI39_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9207810.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            28201..29085
                     /locus_tag="IWI39_000684"
     CDS             28201..29085
                     /locus_tag="IWI39_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9207811.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(29277..29348)
                     /locus_tag="IWI39_000685"
     tRNA            complement(29277..29348)
                     /locus_tag="IWI39_000685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29313..29315),aa:Arg,seq:ccg)
     gene            29408..30742
                     /gene="rpoN"
                     /locus_tag="IWI39_000686"
     CDS             29408..30742
                     /gene="rpoN"
                     /locus_tag="IWI39_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9207812.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            30945..31982
                     /locus_tag="IWI39_000687"
     CDS             30945..31982
                     /locus_tag="IWI39_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9207813.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            32023..33024
                     /gene="gap"
                     /locus_tag="IWI39_000688"
     CDS             32023..33024
                     /gene="gap"
                     /locus_tag="IWI39_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9207814.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            33134..34324
                     /locus_tag="IWI39_000689"
     CDS             33134..34324
                     /locus_tag="IWI39_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9207815.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            34428..35183
                     /locus_tag="IWI39_000690"
     CDS             34428..35183
                     /locus_tag="IWI39_000690"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9207816.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            35299..36597
                     /gene="eno"
                     /locus_tag="IWI39_000691"
     CDS             35299..36597
                     /gene="eno"
                     /locus_tag="IWI39_000691"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9207817.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            36996..38135
                     /locus_tag="IWI39_000692"
     CDS             36996..38135
                     /locus_tag="IWI39_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9207818.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        12645 a         6680 c         8127 g        10696 t
ORIGIN      
        1 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
       61 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
      121 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
      181 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
      241 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
      301 attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
      361 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
      421 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
      481 taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac
      541 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
      601 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
      661 atttgggaca atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
      721 tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc
      781 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
      841 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
      901 tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt
      961 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
     1021 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
     1081 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
     1141 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga
     1201 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
     1261 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
     1321 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
     1381 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
     1441 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag
     1501 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
     1561 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg
     1621 ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc
     1681 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
     1741 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
     1801 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
     1861 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
     1921 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
     1981 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
     2041 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
     2101 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
     2161 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
     2221 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
     2281 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
     2341 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
     2401 gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa
     2461 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
     2521 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
     2581 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
     2641 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
     2701 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
     2761 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
     2821 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
     2881 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
     2941 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
     3001 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
     3061 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
     3121 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
     3181 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
     3241 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
     3301 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
     3361 caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa
     3421 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
     3481 ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca
     3541 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
     3601 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
     3661 ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
     3721 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
     3781 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
     3841 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
     3901 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg
     3961 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
     4021 attccattct tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg
     4081 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
     4141 tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca
     4201 ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa
     4261 atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat
     4321 ttattttact atattggata tatattcatt gaaaatagtg tataacaact ttataatatt
     4381 gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa
     4441 attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
     4501 tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca
     4561 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca
     4621 aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt
     4681 aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct
     4741 tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga
     4801 atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga
     4861 ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac
     4921 gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat
     4981 ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt
     5041 agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
     5101 atgtttactg acacttttac ggattgcagc acgctataat tatttatttg aattagagaa
     5161 agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa
     5221 tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga
     5281 aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg
     5341 tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc
     5401 aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca
     5461 gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta
     5521 ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg
     5581 aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa
     5641 gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt
     5701 ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac
     5761 cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat
     5821 ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc
     5881 ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac
     5941 aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga
     6001 gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta
     6061 tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat
     6121 cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac
     6181 aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa
     6241 aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga
     6301 cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt
     6361 gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca
     6421 cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga
     6481 acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa
     6541 gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa
     6601 ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc
     6661 gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc
     6721 gaggatttta tgcatatcat ctttgctttg aaccgcttca atgagtccat cgttagaaaa
     6781 agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac
     6841 aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg
     6901 agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa
     6961 tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca
     7021 gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg
     7081 gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg
     7141 actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc
     7201 tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc
     7261 cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat
     7321 cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt
     7381 attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt
     7441 tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa
     7501 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
     7561 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
     7621 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
     7681 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
     7741 gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa
     7801 gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa
     7861 gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
     7921 gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc
     7981 ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
     8041 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
     8101 cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt
     8161 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa
     8221 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
     8281 ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa
     8341 tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga
     8401 agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
     8461 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
     8521 aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat
     8581 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt
     8641 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
     8701 tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa
     8761 gaagataacg acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga
     8821 agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac
     8881 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt
     8941 gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga
     9001 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga
     9061 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat
     9121 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct
     9181 gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg
     9241 tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag
     9301 ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat
     9361 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
     9421 acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt
     9481 gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt
     9541 ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat
     9601 cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg
     9661 tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag
     9721 ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat
     9781 gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc
     9841 tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt
     9901 gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct
     9961 gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga
    10021 ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt
    10081 attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat
    10141 gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct
    10201 gattctgcaa ttgcttccag gtacgcaagg tttgtatgga ttcgttattg cctttttgat
    10261 ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc
    10321 tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc
    10381 aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    10441 gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc
    10501 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac
    10561 gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa
    10621 aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa
    10681 atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga
    10741 caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact
    10801 gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca
    10861 ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct
    10921 cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    10981 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    11041 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    11101 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    11161 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    11221 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    11281 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    11341 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc
    11401 tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    11461 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    11521 ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    11581 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    11641 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    11701 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    11761 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    11821 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    11881 tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    11941 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    12001 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    12061 tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    12121 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    12181 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    12241 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    12301 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    12361 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    12421 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    12481 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    12541 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    12601 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    12661 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    12721 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    12781 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    12841 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    12901 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    12961 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    13021 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    13081 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    13141 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    13201 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    13261 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    13321 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    13381 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    13441 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    13501 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    13561 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    13621 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    13681 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    13741 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    13801 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    13861 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    13921 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    13981 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    14041 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    14101 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    14161 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    14221 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    14281 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    14341 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    14401 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    14461 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    14521 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    14581 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    14641 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    14701 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    14761 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    14821 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    14881 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    14941 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    15001 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    15061 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    15121 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    15181 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    15241 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    15301 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    15361 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    15421 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    15481 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    15541 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    15601 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    15661 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    15721 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    15781 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    15841 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    15901 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    15961 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    16021 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    16081 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    16141 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    16201 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    16261 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    16321 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    16381 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    16441 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    16501 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    16561 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    16621 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg
    16681 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    16741 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    16801 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    16861 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    16921 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    16981 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    17041 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    17101 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    17161 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    17221 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    17281 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    17341 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    17401 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    17461 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    17521 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    17581 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    17641 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    17701 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    17761 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    17821 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    17881 gaaattcgaa cgaatgcatc tggttgcgta cgtgtagtaa agcgtctgga atgataaagt
    17941 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    18001 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    18061 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    18121 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    18181 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    18241 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    18301 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    18361 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    18421 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    18481 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    18541 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    18601 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    18661 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    18721 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    18781 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    18841 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    18901 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    18961 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    19021 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    19081 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    19141 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    19201 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    19261 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    19321 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    19381 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    19441 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    19501 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    19561 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    19621 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    19681 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    19741 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    19801 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    19861 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    19921 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    19981 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    20041 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    20101 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    20161 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    20221 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    20281 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    20341 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    20401 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    20461 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    20521 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    20581 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    20641 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    20701 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    20761 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    20821 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    20881 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    20941 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    21001 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    21061 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    21121 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    21181 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    21241 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    21301 tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    21361 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    21421 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    21481 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    21541 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    21601 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    21661 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    21721 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    21781 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    21841 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    21901 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    21961 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    22021 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    22081 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    22141 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    22201 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    22261 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    22321 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    22381 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    22441 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    22501 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    22561 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    22621 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    22681 cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg
    22741 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    22801 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    22861 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    22921 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    22981 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    23041 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    23101 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    23161 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    23221 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    23281 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    23341 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    23401 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    23461 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    23521 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    23581 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    23641 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    23701 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    23761 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    23821 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    23881 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    23941 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    24001 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    24061 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    24121 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    24181 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    24241 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    24301 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    24361 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    24421 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    24481 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    24541 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    24601 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    24661 atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa
    24721 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    24781 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    24841 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    24901 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    24961 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga
    25021 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    25081 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    25141 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    25201 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    25261 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    25321 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    25381 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    25441 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    25501 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    25561 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    25621 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    25681 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga
    25741 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    25801 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    25861 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    25921 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    25981 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    26041 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    26101 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    26161 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    26221 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    26281 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    26341 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    26401 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    26461 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    26521 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    26581 caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa
    26641 cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt
    26701 actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc
    26761 gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag
    26821 gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa
    26881 gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc attagatagt
    26941 tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag
    27001 ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg
    27061 gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact
    27121 atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta
    27181 cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa
    27241 aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg
    27301 ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct
    27361 ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa
    27421 tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat
    27481 cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa
    27541 tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga
    27601 aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta
    27661 ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag
    27721 gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa
    27781 gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt
    27841 tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta
    27901 ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc
    27961 tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga
    28021 aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac
    28081 gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt
    28141 tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata
    28201 gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa
    28261 attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    28321 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    28381 gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa
    28441 tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc
    28501 tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa
    28561 aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg
    28621 aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg
    28681 gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa
    28741 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
    28801 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    28861 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
    28921 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    28981 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
    29041 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    29101 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    29161 taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt
    29221 ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg
    29281 tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact
    29341 aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga
    29401 caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc
    29461 catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt
    29521 ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg
    29581 gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct
    29641 aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt
    29701 gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt
    29761 gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat
    29821 ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg
    29881 tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc
    29941 ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc
    30001 acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt
    30061 gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt
    30121 ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc
    30181 aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac
    30241 tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca
    30301 acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt
    30361 tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga
    30421 tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg
    30481 tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac
    30541 agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga
    30601 caaaaacaag ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc
    30661 gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa
    30721 aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta
    30781 gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt
    30841 tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt
    30901 ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa
    30961 aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt
    31021 tacgaaatat ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga
    31081 tcacagaacg agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca
    31141 cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa
    31201 acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg
    31261 gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg
    31321 cattcggcga agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca
    31381 tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa
    31441 ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg
    31501 tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac
    31561 tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta
    31621 tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg
    31681 cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag
    31741 caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata
    31801 aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa
    31861 tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac
    31921 aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt
    31981 aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt
    32041 attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga
    32101 atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa
    32161 tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac
    32221 gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc
    32281 gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct
    32341 gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat
    32401 gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc
    32461 tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa
    32521 tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact
    32581 ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc
    32641 gttcctaact caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt
    32701 aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta
    32761 acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca
    32821 aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact
    32881 tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta
    32941 gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact
    33001 ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt
    33061 acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc
    33121 aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt
    33181 tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg
    33241 tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct
    33301 tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc
    33361 agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg
    33421 cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga
    33481 aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg
    33541 taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg
    33601 tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt
    33661 gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat
    33721 tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc
    33781 taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg
    33841 aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat
    33901 cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc
    33961 aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga
    34021 catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt
    34081 ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt
    34141 ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc
    34201 cgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg
    34261 cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa
    34321 ataaacgacc aagtttgctc aattctgacg agtaatcgaa caaaaggtga agggagtttt
    34381 catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta
    34441 ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag
    34501 cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat
    34561 tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa
    34621 actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg
    34681 atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa
    34741 cagacgaaga catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc
    34801 tatgctgtgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag
    34861 gacaaattac agctggttta gctgatcttt cagctgaaca ggtgtcaaac atggttattg
    34921 cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg
    34981 aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag
    35041 cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa
    35101 aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt
    35161 cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc
    35221 actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac
    35281 tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac
    35341 tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc
    35401 ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga
    35461 gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc
    35521 attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg
    35581 atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt
    35641 tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta
    35701 ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca
    35761 catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca
    35821 ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa
    35881 gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca
    35941 aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt
    36001 aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt
    36061 gtttacgtac tagctgattc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc
    36121 tacgaagaat tagtttctaa atatccaatc atctcaatcg aagacggatt agacgaaaac
    36181 gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt
    36241 gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct
    36301 aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc
    36361 gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa
    36421 gattcaacaa tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct
    36481 ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt
    36541 ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc
    36601 ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta
    36661 agtaatcgaa ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc
    36721 ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag
    36781 ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta
    36841 tgagcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag
    36901 tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa
    36961 atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga
    37021 ttttacaaga gatcatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct
    37081 atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg
    37141 gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat
    37201 ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg
    37261 cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct
    37321 tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa
    37381 cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca
    37441 cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca accaattaca
    37501 gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag
    37561 cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta
    37621 cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa
    37681 aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc
    37741 atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg
    37801 cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga
    37861 tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca
    37921 ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag
    37981 ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta
    38041 ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat atgatcgaaa
    38101 aatatcaagg aattatttta tcctacttag cgtaagattt ttttaata
//
LOCUS       DACDOA010000015        36569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDOA010000015 DACDOA010000000
VERSION     DACDOA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.015"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWI39_000693"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWI39_000693"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9207819.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWI39_000694"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWI39_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9207820.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWI39_000695"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWI39_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9207821.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWI39_000696"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWI39_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9207822.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWI39_000697"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWI39_000697"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9207823.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWI39_000698"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWI39_000698"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9207824.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWI39_000699"
     CDS             complement(8053..8523)
                     /locus_tag="IWI39_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9207825.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWI39_000700"
     CDS             complement(8569..8994)
                     /locus_tag="IWI39_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9207826.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWI39_000701"
     CDS             complement(9061..9330)
                     /locus_tag="IWI39_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9207827.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWI39_000702"
     CDS             complement(9341..10936)
                     /locus_tag="IWI39_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9207828.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWI39_000703"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWI39_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9207829.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWI39_000704"
     CDS             complement(14489..15049)
                     /locus_tag="IWI39_000704"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9207830.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWI39_000705"
     CDS             15402..16376
                     /locus_tag="IWI39_000705"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9207831.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWI39_000706"
     CDS             16546..17946
                     /locus_tag="IWI39_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9207832.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWI39_000707"
     CDS             complement(18024..18746)
                     /locus_tag="IWI39_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9207833.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWI39_000708"
     CDS             complement(18825..19331)
                     /locus_tag="IWI39_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9207834.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWI39_000709"
     CDS             19454..21253
                     /locus_tag="IWI39_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9207835.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWI39_000710"
     CDS             complement(21321..22895)
                     /locus_tag="IWI39_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207836.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWI39_000711"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWI39_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9207837.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWI39_000712"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWI39_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9207838.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IWI39_000713"
     CDS             complement(24672..25310)
                     /locus_tag="IWI39_000713"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9207839.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IWI39_000714"
     CDS             complement(25311..25961)
                     /locus_tag="IWI39_000714"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9207840.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWI39_000715"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWI39_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9207841.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWI39_000716"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWI39_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9207842.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IWI39_000717"
     CDS             complement(29104..29844)
                     /locus_tag="IWI39_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9207843.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWI39_000718"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWI39_000718"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9207844.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IWI39_000719"
     CDS             complement(31215..32153)
                     /locus_tag="IWI39_000719"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9207845.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWI39_000720"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWI39_000720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9207846.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWI39_000721"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWI39_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9207847.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IWI39_000722"
     CDS             complement(35313..35618)
                     /locus_tag="IWI39_000722"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9207848.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWI39_000723"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWI39_000723"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9207849.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6705 g        11989 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DACDOA010000016        36460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDOA010000016 DACDOA010000000
VERSION     DACDOA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.016"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(388..1854)
                     /locus_tag="IWI39_000724"
     CDS             complement(388..1854)
                     /locus_tag="IWI39_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207850.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2145..4229)
                     /gene="cadA"
                     /locus_tag="IWI39_000725"
     CDS             complement(2145..4229)
                     /gene="cadA"
                     /locus_tag="IWI39_000725"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9207851.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4371..4550)
                     /locus_tag="IWI39_000726"
                     /pseudo
     CDS             complement(4371..4550)
                     /locus_tag="IWI39_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(4754..5113)
                     /locus_tag="IWI39_000727"
     CDS             complement(4754..5113)
                     /locus_tag="IWI39_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9207852.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5143..5484)
                     /locus_tag="IWI39_000728"
     CDS             complement(5143..5484)
                     /locus_tag="IWI39_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207853.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5481..6155)
                     /locus_tag="IWI39_000729"
     CDS             complement(5481..6155)
                     /locus_tag="IWI39_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9207854.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7331..8629)
                     /gene="asnS"
                     /locus_tag="IWI39_000730"
     CDS             complement(7331..8629)
                     /gene="asnS"
                     /locus_tag="IWI39_000730"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9207855.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8669..9859)
                     /locus_tag="IWI39_000731"
     CDS             complement(8669..9859)
                     /locus_tag="IWI39_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9207856.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9880..10407)
                     /locus_tag="IWI39_000732"
     CDS             complement(9880..10407)
                     /locus_tag="IWI39_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9207857.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10454..13243)
                     /locus_tag="IWI39_000733"
     CDS             complement(10454..13243)
                     /locus_tag="IWI39_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9207858.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13390..13590)
                     /locus_tag="IWI39_000734"
     CDS             complement(13390..13590)
                     /locus_tag="IWI39_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9207859.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14043..14363)
                     /locus_tag="IWI39_000735"
     CDS             complement(14043..14363)
                     /locus_tag="IWI39_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207860.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14866..15984)
                     /locus_tag="IWI39_000736"
     CDS             complement(14866..15984)
                     /locus_tag="IWI39_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207861.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16455..17762
                     /locus_tag="IWI39_000737"
     CDS             16455..17762
                     /locus_tag="IWI39_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9207862.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17883..19082
                     /locus_tag="IWI39_000738"
     CDS             17883..19082
                     /locus_tag="IWI39_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9207863.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19109..20377)
                     /locus_tag="IWI39_000739"
     CDS             complement(19109..20377)
                     /locus_tag="IWI39_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9207864.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(20547..21188)
                     /locus_tag="IWI39_000740"
     CDS             complement(20547..21188)
                     /locus_tag="IWI39_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9207865.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(21597..22100)
                     /locus_tag="IWI39_000741"
     CDS             complement(21597..22100)
                     /locus_tag="IWI39_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9207866.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(22223..22987)
                     /locus_tag="IWI39_000742"
     CDS             complement(22223..22987)
                     /locus_tag="IWI39_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9207867.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            23095..23370
                     /locus_tag="IWI39_000743"
     CDS             23095..23370
                     /locus_tag="IWI39_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9207868.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23930..24880
                     /gene="yidC"
                     /locus_tag="IWI39_000744"
     CDS             23930..24880
                     /gene="yidC"
                     /locus_tag="IWI39_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9207869.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(24926..25327)
                     /locus_tag="IWI39_000745"
     CDS             complement(24926..25327)
                     /locus_tag="IWI39_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9207870.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(25341..26150)
                     /locus_tag="IWI39_000746"
     CDS             complement(25341..26150)
                     /locus_tag="IWI39_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9207871.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(26137..27027)
                     /locus_tag="IWI39_000747"
     CDS             complement(26137..27027)
                     /locus_tag="IWI39_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9207872.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(27040..27519)
                     /locus_tag="IWI39_000748"
                     /pseudo
     CDS             complement(27040..27519)
                     /locus_tag="IWI39_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(27695..28636)
                     /locus_tag="IWI39_000749"
     CDS             complement(27695..28636)
                     /locus_tag="IWI39_000749"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9207873.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(28633..29628)
                     /gene="lacD"
                     /locus_tag="IWI39_000750"
     CDS             complement(28633..29628)
                     /gene="lacD"
                     /locus_tag="IWI39_000750"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9207874.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(29621..30817)
                     /locus_tag="IWI39_000751"
     CDS             complement(29621..30817)
                     /locus_tag="IWI39_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9207875.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPVFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(30835..31563)
                     /locus_tag="IWI39_000752"
     CDS             complement(30835..31563)
                     /locus_tag="IWI39_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9207876.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(32004..32978)
                     /gene="lacD"
                     /locus_tag="IWI39_000753"
     CDS             complement(32004..32978)
                     /gene="lacD"
                     /locus_tag="IWI39_000753"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9207877.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(32975..33433)
                     /locus_tag="IWI39_000754"
     CDS             complement(32975..33433)
                     /locus_tag="IWI39_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9207878.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(33430..34893)
                     /locus_tag="IWI39_000755"
     CDS             complement(33430..34893)
                     /locus_tag="IWI39_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207879.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(34875..35807)
                     /gene="pfkB"
                     /locus_tag="IWI39_000756"
     CDS             complement(34875..35807)
                     /gene="pfkB"
                     /locus_tag="IWI39_000756"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9207880.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35949..>36460
                     /locus_tag="IWI39_000757"
     CDS             35949..>36460
                     /locus_tag="IWI39_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9207881.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSL"
BASE COUNT        10664 a         7842 c         6205 g        11749 t
ORIGIN      
        1 gaataaatgg tgttcaatca gctgaccttc agcattagct caaaaagcgg caaaatatga
       61 ggagctattt ttccgtttgt ttctctaata ctcaggtcaa atcgctgatt ttacaacctc
      121 ttccttaaac ggtgttccaa agcttgcatc tgcggtaata agcccagcca aacgaaaaat
      181 atgaggggct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
      241 cctctgattc acggtgttcc agaagtagct tcttcgaaaa acaagccgaa acttcacaaa
      301 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      361 caacctcttg taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat
      421 ttctttgttt ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac
      481 gattggtgtg atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata
      541 atctactttt ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc
      601 accaaagata aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt
      661 ataagcttta ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac
      721 aactgagttc aagttcgtca accattttac taattcattc atatctttga taccaaaaat
      781 cggcaacaaa ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt
      841 acctttacga ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa
      901 caagattttt agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt
      961 tgtcaatgcg taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg
     1021 aaaagcactg tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag
     1081 catacccatt gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat
     1141 catcccttta ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt
     1201 ttccgcccct ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa
     1261 tgttggaata tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt
     1321 gatgaacggt gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt
     1381 tccggccagt ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa
     1441 ggcaagaaat ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat
     1501 atttccattt ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc
     1561 caaataagga atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt
     1621 agatgtcgta ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc
     1681 taattgtcca acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga
     1741 ggtgacaact gatactcctt gctgagcata gttattaact acattcccca tgccccaaac
     1801 catgttaaat gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc
     1861 tctcctttat gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt
     1921 acaagacagt gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat
     1981 tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
     2041 ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc
     2101 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt
     2161 tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca
     2221 aatactgcat ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt
     2281 atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg
     2341 attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt
     2401 gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt
     2461 gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc
     2521 tctaaccgct gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact
     2581 tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacatgac tgtgttcttc
     2641 acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca
     2701 ataacaagat aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct
     2761 tgacttaaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact
     2821 tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt
     2881 ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac
     2941 tgttctgcac tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc
     3001 tggatcgaaa agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt
     3061 tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt acttgcgcct
     3121 ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc
     3181 actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt
     3241 ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg
     3301 aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct
     3361 aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta
     3421 tttttattga taaatccgct taatactgtt tcaccaacat atacagattt agggactgat
     3481 tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac
     3541 ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc
     3601 acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat
     3661 tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga
     3721 taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta
     3781 gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc
     3841 ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt
     3901 ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg
     3961 aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct
     4021 gttactttga catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta
     4081 gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct
     4141 tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa
     4201 tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac
     4261 atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa
     4321 aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc ttattctttc
     4381 tttttctctt cgatcattgc ccctgctctt atccaacccg cacttgcctt cttgatcttg
     4441 acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt cattaatttt
     4501 tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat attcgaagct
     4561 ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc aactttccat
     4621 aaacagtaat cccatataaa taaaagacgt aaaagtcaca tgggagaact tatacaggaa
     4681 aacacataga gtcaggtgaa atacatgtat cccacctgac tctatgcgtt ttctaacctt
     4741 tattattctc tttttataca atttctgcac cgaatggcat caacgccaat cgagcaagtt
     4801 tgaaagattg cttggcaaaa ggtatcccta tgatcgtgat acatagaagg atgccgctga
     4861 cgacatgtgc gagagcaatt ggtaggcctg aaacgatcag ccagatgata ttaacgatca
     4921 atgacatgcc agactggtga taaattactt ctttgccaaa aggcgcaaaa gaaagggagg
     4981 cgatcttaaa acattgcata ccgattggga tacctatgat cgtgatacac caaagtatcc
     5041 ccgctgctaa ccagctaaga ccaccgatta gtccgccgaa tataaaccag atgacatttc
     5101 ctaaacaact cattgtattt ctccttctat tactgttttt gattaaaact gggcaaaaat
     5161 tgattttatt cttctttttt tagcttccag catcaagtat tctgcaaatt ttttttggat
     5221 ttcttccgta ttttcttttg ttactaatgt gtgttggatt agttttcctg atggtcgctg
     5281 atccacccgt tcttctgact gttgcacgtc cgcttgcgca aatccgataa tccgtttttc
     5341 ttttgttaca gcaacgagca atagtttatt cttatcattt aggtcagctg ccgtttttgc
     5401 ttttacgaat gattctggat gatcagtaat cagctgattg atttgaggta aatcttcttt
     5461 tactgcccac cgtatttcca tcatttctca cttttctttc gctcattttt atctccacta
     5521 aaaaaatcga ctgcggcaat tgcatctatc cagctttgtg caatgatctc atgtccgcca
     5581 atcgttgggt ggaccccatc ttctcttgta tatttcgaaa aaccataagc aatccctaaa
     5641 gcattgaata tgccatctaa aggaaccagc aatgcgccat attccttagc aagtttacga
     5701 acaatttgga ttttagggtc taagtcttta cgccagccat gacggtcgtc ggttatagga
     5761 agtacaaacg gctccatcaa gatgatgttg cagatttccg ctgtttgaag ttcatttagt
     5821 aattttcgat aaacggattc aaattgctgc gcagatttct cagttccgaa tatagcctga
     5881 tcgctagtat tatgccaggt atcattgatt ccgatcaaaa tcgatacata gtcaggttgt
     5941 aaagctaaac aatcttcttg ccagcgtact tgcagatctg caattttgtc tcctcctatc
     6001 ccacggttca tgcattcaaa tttcatcgtt ggatattggg agattagttt tccggcaatc
     6061 aaaagtggat aacctttacc tagatcagtt gcttctgctc ggtctcttcc tgcgtctgta
     6121 atgctgtcac cgataaataa tatacgtttt ttcatttgtc aaaaactcct ttttgaataa
     6181 aacgatgatt ttcttcctta tattatccca taagccctat caagacgaaa gagaaaatat
     6241 atgtataaaa aatgacacca gcctagtccc ttcaatcaat tgaatgaacg ctcatcaagt
     6301 gtctagtttt ttatactttt tttcttaacg tgccggattc cccttgattc cttcgttgtt
     6361 tggtccagag aaatcgatca cacctaagct tccgtcaatc agaccactga tgacgccatc
     6421 agaacatata tttataggct catctaagac ttccagtaaa tgttctttta ctaatatctc
     6481 ttctagattg cttgggatag attggtcata actttatctg gtccgatgat ttcaaggatg
     6541 aattaaactt acatatggat cattgcatgc tgaaaatacg gacctactcc atcttcgaat
     6601 gttactatta gtttgttttc aggtactaga tgctgcttta tttcaacagc agaagcatca
     6661 tcaaatcaga tttccatttg aataaccttc tttgtatatg tttgattgat tgcgatttcg
     6721 ttatagaacc ttgtgaatca aatagcaatt tcttttgctt tcttctcttg tctgctggaa
     6781 acttccttta tcccgtattt taaaaaaaga actgcgagag ttcgcagttc ttttttttaa
     6841 ataaacgttg ctctccggcc aactcctcgg tcacaagtca caatagtcat caaacatgaa
     6901 aaactgtttt ttgaaattac tccttgtgac tcggagctga acggcaggcg cacaacctct
     6961 tgatataaac ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac ggtaaaatat
     7021 gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg atttcacaac
     7081 ctcttcctta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
     7141 atgtggaaag ccatttccac aaaatttttc ttaatactcg aaactaaaca gctttttcac
     7201 aacctctcca ctaaacggta ttcaaaagct tacatctgcg gtaataagtc ccgccaattc
     7261 aaaaacatga acagctgttt ttgcttggct gttctaatta cttgatgcag aacagctttt
     7321 tcatcacctc ttatggataa attcggttca acaaacgtgg gaatggaata gcttcacgta
     7381 catggtcgat accagcaatc caagtgactg ttcgttcaag gcctagacca aaaccagagt
     7441 gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt tcatccaaac
     7501 cgtgattacg gatttgttct agcaagaaat catagtctgt tgcccgttcg cttccgccaa
     7561 taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg tcttctcttg
     7621 ttggatgtgg tttcatgtag aatggcttga tggcttttgg atagtttagg ataaagacag
     7681 gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct tcgccccatt
     7741 cgatgtcatc aaatccattt ttcttcagta attcgattgc atcatcgtaa gtaatacgag
     7801 ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt acatctaatg
     7861 catattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt tcctgtactt
     7921 ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag aattcgatca
     7981 aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa aagacttttc
     8041 cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa taagcttttt
     8101 gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcagaa ccagtcaaga
     8161 tcggcggatc gattttgaca aaatgatgtt gattaaagaa ttcatacgtt gcacgaatga
     8221 tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgcagccat aaatgacgat
     8281 gatccatcaa gaaatctgtt ccgtgttctt ttggtgtgat cggataatcg tggctctcgc
     8341 ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacga ctgtcttcac
     8401 ggatttctcc agttactaac acggatgttt cttggtgtag atttttagct gtttggaaaa
     8461 cttcttccga cacttcgctt tttacaacta caccttggaa aaaggctgtt ccgtctctta
     8521 attgtaaaaa tgcgattttt ccgcttgatc gcttatttgc gacccaagcg ccgattgtta
     8581 ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg tatgactctc
     8641 cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca ataaattgct
     8701 tgattctgcg taccgcttct tctaacgtag cgcgatcagt ggcataactc atgcggacat
     8761 tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct tctaaaagat
     8821 cttctaccca ttgtgtgaca ttttcatact tacacatgcg tagcgtttct tttacattag
     8881 gaaaaagata gaaagctcct tgcggctttt caagagaaac acccggcagt tctgccacaa
     8941 gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct actgtatctt
     9001 gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga ttgctagttg
     9061 attgggaagc aacagcgatc attccattga tgatcacagg atttcccacc gcataaccaa
     9121 tgcgccagcc tgtcatcgca tatgttttag acacgccgtt gatgatgatg gtctgatttt
     9181 tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa actaaacggc
     9241 cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca ccgattgcct
     9301 gcagttcatt cttgctataa atcattccag tcggattcga tggagaattg atgattacgg
     9361 cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc cagctattgg
     9421 attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc tcaccgtaac
     9481 tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc gtttggaaaa
     9541 gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt tcatagacaa
     9601 gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg attcctgctg
     9661 ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct tcttggatgt
     9721 tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct ctgccttttg
     9781 cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct aatcgttttg
     9841 ttctttttga taactccatc ccattcattc ctttaccttt taaattccct tgattgtctt
     9901 gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat ttgtttcttt
     9961 tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt tttcgatttg
    10021 gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca gtccctttgt
    10081 ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga tttcttttcc
    10141 tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc aataaaaatg
    10201 atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt tcttcgcctg
    10261 caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga ccgctaataa
    10321 aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct gttttttctt
    10381 caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt aattatgaaa
    10441 aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg atatcctgat
    10501 agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca tggatgatcc
    10561 gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg ccacgatctg
    10621 tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct ttaggtacgg
    10681 attcctggta aaaaggattg atcccctctg attctaaata agcgttccta gctttgacca
    10741 tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc aaagcatctc
    10801 caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt ttttctgaca
    10861 aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct agtatttctc
    10921 gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa acatcatgag
    10981 aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta agtgctgtct
    11041 ctaaataatc gatatatgct tgattgctga cttgatggac ggcaggtgcc tcttttggca
    11101 catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta agaggtgctg
    11161 cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc agcgttcctc
    11221 caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac atagccgctt
    11281 gaaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg aaccaatgga
    11341 tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtattgc ttgtctaatt
    11401 gatcaaatag gctatataaa cttgctaaat cgatccgcat ctggtttgtc cagctttctt
    11461 ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctggatctcc tgataaaaaa
    11521 gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccaac atctcttttg
    11581 tcatcatgac ttcttctgga tactgtccgt taggttgctt gccgctcact aaactcatta
    11641 atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct cgataaatcg
    11701 ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg tcaaacagct
    11761 gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt aatcgtttgt
    11821 catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac aaaaatggac
    11881 tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca tgattgacaa
    11941 tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct tgttcgtaaa
    12001 atgactgatt tcttgctaaa aatccaatac ctctgtgcgc gatatctgca aagaatgaat
    12061 ggttcaaatt tgttaaatgt aattcatcca aatcaccagt cttcgtttgc gtcagccaaa
    12121 ctaagaccgc catttgatac aaagcatatt gtttttgttc tggcggttcc acaagtgtgc
    12181 ctttaaaacg ctgcaaatca atatagtggc gatagctttt gataattaca ggttgtatgg
    12241 gctgatctag caattgattc agcaatggaa tgtctttttc tatgatctgc tgctgcaaaa
    12301 ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa ctcaatggca
    12361 gcagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa tcttttgatt
    12421 catcttttgt gaaatggtcg tagaccgcat tcatcagtct gttctgttct tttcgataca
    12481 tcagtgtttt accaaacatt ttttcttttg cttgtttttt tcttggatat gtccgctcgc
    12541 cataatggac ggatgtgaat agttccactt cttttttctg caaagctagc ccatcgacga
    12601 tttctaatga gggatcaagt ggctctggtt tttctttcat ttcttcaatc acatgataaa
    12661 tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc tctaacgtga
    12721 tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct gtcacttcgg
    12781 catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct gacaaatcag
    12841 ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact gtatcgatgc
    12901 ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa aaattataat
    12961 caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa atcgtttggg
    13021 cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct gttaaatgct
    13081 ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt gaagtgatcc
    13141 gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt ggattcgtcc
    13201 cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct tcctcttctc
    13261 ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact ctcatcgctt
    13321 tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt attttttagt
    13381 ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc cttcttcaac
    13441 atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt ggatagctga
    13501 gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc ctttttctga
    13561 gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt atcaacacat
    13621 attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac aagctaccac
    13681 agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa aagctagagt
    13741 ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg aaaaaccgaa
    13801 ctttttttta aaataaacga catattgttc agttggtgtt tttttgctct gatgttcttt
    13861 tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa cggtcgaggc
    13921 tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc ctatgacagt
    13981 taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt ttttgctcat
    14041 atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg agtaaaagca
    14101 aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca ggtcggacag
    14161 taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg aatcgcagaa
    14221 tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt aataggaaac
    14281 cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag agacagcaaa
    14341 caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc ccaattaaaa
    14401 tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt tttgtatcat
    14461 aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt gttccagaag
    14521 tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt tttgtcaatc
    14581 tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa cggtgttcaa
    14641 aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct atttttgctt
    14701 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca cggtgtttaa
    14761 tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta tttttccgtt
    14821 tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat aacgaagaat
    14881 cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca cttttccgcc
    14941 attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat attctgtttc
    15001 aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat gtgacacttt
    15061 tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct tatctaagag
    15121 tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct tcgccccttc
    15181 ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt ctgcacaata
    15241 atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag caaataaata
    15301 aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa ctgcttggta
    15361 atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat ggaaagttac
    15421 tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac ctgtcagttc
    15481 agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata cttcttttac
    15541 ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca ataaataata
    15601 agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat cacttacata
    15661 gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag cagttataat
    15721 gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca aattttcaat
    15781 tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac gtttctttgc
    15841 ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat gggcgacatg
    15901 cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca attgtgacaa
    15961 taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat tcctttgttt
    16021 ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct aaaaaaataa
    16081 aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata atttcctcaa
    16141 ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa gaaaaagaat
    16201 aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta ttatcagttt
    16261 atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc ctgtaaaagt
    16321 acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt tttttcagaa
    16381 acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac aaattaatta
    16441 caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg gctagtctca
    16501 ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat actttcaaag
    16561 taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt tatgatcaag
    16621 atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc
    16681 ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt tcagattatg
    16741 cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa gaaaacacct
    16801 acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct tcaatggttg
    16861 ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg aaagaacaat
    16921 tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg aataactctt
    16981 atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa atgtctgctc
    17041 aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata ctggatgtat
    17101 ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa atggtcaact
    17161 ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga ttaaaaactg
    17221 gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac gatcaacgaa
    17281 tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc cgtttcaatg
    17341 aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag cttggaaaag
    17401 ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag
    17461 tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg gatactggaa
    17521 aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag atccaagcac
    17581 ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa gataaattag
    17641 gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa accgtagaaa
    17701 aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt aacttgtttt
    17761 aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg ctttttctag
    17821 tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat
    17881 ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg gtattctcca
    17941 tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc agtacatgga
    18001 cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg accttttatt
    18061 ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca gaaaagcgaa
    18121 ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc tacttgtttg
    18181 tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg atcacgatcc
    18241 cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt ttttggaacg
    18301 gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg ttgatactag
    18361 ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat ttaccacaat
    18421 ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt atcgtattct
    18481 acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac aaccaagact
    18541 tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct cttcttgcca
    18601 agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca ttaaatccgc
    18661 ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa gcaatggcga
    18721 tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc gaaaccaatt
    18781 cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag caaatgaaag
    18841 gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct ggtctctatg
    18901 cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc tattacttgc
    18961 ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga
    19021 tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact ttacttttct
    19081 aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttt attctcttga
    19141 cgattggcaa gaatagtatt ccttttttga cggctacttg ctgtgcagaa taaataccgc
    19201 tgtttcctga acacataaag ctgatcaagc agatcataaa gaagtaagca gcagcttgtg
    19261 aaccaaataa ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc gcaccagaaa
    19321 ataccccgat aaaacctaat ccagcaagaa aaggaacaga aacatggagc aaaggcgcta
    19381 gtactgcgcc aagcgttgca ccaatctcaa atagtggtgt cacttctccc ccttggtacc
    19441 ccgcacctaa agacaagacc gtgaagaata atttcccaat aaaatcataa gcatgtgcct
    19501 gtccattaaa tgattcctgc agtaacggaa gactcaaccc aaggtaacgt tgtgtttgca
    19561 aaatcaacgc tgcggctaca actactgctc cacctatcgt atttcgccaa acaggatgag
    19621 gaatccattg cgcataccgc gcttttaaaa agacgatcga acggctgaat acccaaccaa
    19681 tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag
    19741 gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc atgaaatttg
    19801 caaaaagcgc agcaaagaag ctaggaaata ttgcttctgt tcggacttta ccgattgcca
    19861 acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg
    19921 cactgattcc actgataacc aaaatctccc gttctgcttt atccaatcgg aataaatgcg
    19981 ctatattatc agctaatgct ccccccattt gaactgctgt tccttcacga ccaacagatc
    20041 ccccaaataa atgggtagtg atggtcccaa acaaggtcaa tggaatgagc cgcaaaggga
    20101 tcttctcttc tcctccattc ccttgatcga tcactaagtt attcccacga ctagaaagac
    20161 cgccatgata cgcataaaga taagcaaaaa gcgcgccgct gactggcaat agataaagta
    20221 gccaaggatg tgctagtcgg atatctgtca cgaacgtcag gctatttaag aaaaaagcag
    20281 acaagctccc catgaatact ccgagaatcg ctgtgaccac cagccacttc aacatgtaca
    20341 tcaaaatacg cactggactc ggtaattgtt tttccataaa aaattcctcc tcaaataaaa
    20401 aaacgcctac taaagaaagg ggctggcccc agactttagt aggcgtcatt agctatctta
    20461 cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt
    20521 ttctgtaaaa aacaataaaa ttatcgttat ttctttacac gttcagcaaa ttcatagaaa
    20581 ggagccaatt tcgttaactg ttgattacat tgatcactat catgacaaat ataattcccc
    20641 tttttcgtat acgtgccatc agaaccggct ttcgttgtag aaagaaagag cgagacattc
    20701 gatgttttct gacagatcgc acagattccc ttattaatcg ttggtgataa agtaccgctg
    20761 atccctttta attttccatc ttcgtaataa accatgaatt ttttttgcgt ccctggatca
    20821 ttccaaccga tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg ttgtttgagt
    20881 tttttcactt ttcggaataa tttctcgatt tgtggtttgc tcggctgcat aaacggctgg
    20941 acgatttgct tcagctcctc caatgctcgt tctgcttgcg tttttgtcag ttttacatct
    21001 aaaactgtcg ttaaaaacgt atcgatttct gatggttcgt aacctaatac atcgcgaatc
    21061 ttttcagctg ctagagcctg aaccgtttgt accgtttttg agtcattgac agatgagtac
    21121 gcatttaata actgctctac ttctcttttt ataaaaaaat attgatatgg tttcattgtt
    21181 tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta gaaaaaagtg
    21241 atcttctgca aagtatgtgt tacctgaaga tcccggactc ttttaaataa gatacgtttt
    21301 gcatcttatc atcaccccct ttaatgttca ttcttctatg tacatgattc attcgataaa
    21361 taaaagtaaa ccgtatcttg agcagatagt caactgcaaa cagaaaaagg actgtgacaa
    21421 aagtcgtgtc aaagtccctt tctacaaaca aacggtgttc cagaagtagc ttcttcaaaa
    21481 aataagccgg aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    21541 tgaagctgac ccacttctgt cacaacctca atatggtcta ttattggttt tacctgttaa
    21601 atcgaggatt cttttccgaa gtaacgttgc catctcttac gcatacttgc tgacataggc
    21661 tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata tccttctttg
    21721 tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc tcgctcaaga
    21781 tccgacaaat cggtgatctt ttctgcattc tttacagcga ttcttggatg gatatacgca
    21841 tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt
    21901 ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag
    21961 taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct
    22021 aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata ttcttcgtct
    22081 tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct
    22141 gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg gataaaatag
    22201 ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc cctgccgcaa
    22261 ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc tgatcagtca
    22321 aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt gcgaagttat
    22381 ctgatttttc tactttgtct aaggcgtgtg cttcttcatt taattctgtg ccatacactg
    22441 taaacccttt gtctttcatt aaaggaatca cttctgtcaa atctttttgc aacacaggca
    22501 aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata tctgccgttc
    22561 ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc atcgtcccta
    22621 catttccagg atcttgtaca ttatctaata acagccatcc tcctgaaaaa tctggcgtgc
    22681 cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga tccgatacag
    22741 catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg gccagccagt
    22801 ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct gcatcagacg
    22861 acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt tcttcccgat
    22921 atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct
    22981 ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga gcttgtttca
    23041 tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac gaacatgcgc
    23101 aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta catgaccaag
    23161 atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga cggctcagtc
    23221 acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga agtcaagaaa
    23281 tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc gatgattgag
    23341 gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct gcatcaagca
    23401 ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga cttaatgccg
    23461 tagatgcaag cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta gttttgagta
    23521 gtaaggagaa ttttgtgaaa atggctttcc acatttttgc ataaaatttg acttaatacc
    23581 gagaaactga cttttgaaca ccgtcgataa ggttgttttt gagacgcttt gcctcaagta
    23641 ttaaagatag gtagtaaaaa agctgatcta taacttgcaa taaatttgct taacccatat
    23701 tttccagaca gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca cttggaatca
    23761 ctcttttctt tccaagtatt tctttcttga ttttttgtct ttttttcaaa atttttcgct
    23821 acgtaaaaga acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt
    23881 ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg
    23941 gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt
    24001 gcggatggat tcaaacggaa accctgacac ttcgggaatc gtttatcgag tactcgtcca
    24061 tcctatgggc caagcgatta catatctagt tgagaatttc aattggtctt atggctgggc
    24121 agttattgca atgactgtga tcgtccgtat catcatcttg ccactaggta ttagtcaatc
    24181 gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc
    24241 ccaacaaaaa ttgaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat
    24301 gcagcaagta taccgcgaaa acggattgag tatgaccggc ggaatcggct gtctgcctct
    24361 attgatccaa atgccgatct tctctgcctt gtactttacg gcacgttata cagaaggtat
    24421 tcgggaatca tctttctacg gcatcgattt aggctcacca agtatggtac ttgttgtcat
    24481 tgcaggggtc gcctatctgc ttcaaggata catctcaacg atcggtattc ctgaagaaca
    24541 gaaaaagacg atgcgtacga tgctaatcgt ctctcctgct atgatcgtct tcatgtcgat
    24601 cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca
    24661 aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg cagaagagtt
    24721 gaaacaaaac ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag aatcagcaaa
    24781 gccagctaac aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca gtacaaacaa
    24841 caaaggccgc aacgcaggaa agcagcagaa gcggaaataa aaacatagga caaaaaagat
    24901 gagagctaag ccctcatctt ttttgttact cttcctcttc tgctattcca ttaattaatt
    24961 cgttgacaga gacaatgctt tcacatcctg cacgaacaaa ctcgggctct gaaccaatca
    25021 tttgtgagtt gataaattca atgaccatcg gaagattcat cccagcataa agagtaatct
    25081 gttgtccttc cattattagt ttagaaatga tatttgcagg tgtaccacct aaaagatcgc
    25141 atagtaccac aaaatcattt aacgaatctg tgacagtgag aaacttttgt ttgtatgttt
    25201 cagtgccatc ttctggtaat aaaggtaacg tgtagatatc ctcttgaggc cccatgatca
    25261 tttctgtact ttttttcaat tcctcacaaa attttccgtg actaatcaat accaattgtc
    25321 tactcatttt tcgctctccc ttatttcgaa atcacaccca atgcagataa agcaacacac
    25381 acaattaata cgataaaaat cgctcgagta ggagtcatgt ttttctttcc taataaccaa
    25441 taaacaccac cgacgatccc agcaggtaaa attttaggta agatcatatc gatatagttt
    25501 tgcatattca atgtcacatc gccaatcgtt ggtgcccatg aaaatttaac attgatcatt
    25561 gtcgcaacta atgcccctac catgaatacc cctaataaag tggctgcgtc cgttaatgca
    25621 gttaagcgag attgcatagt agtaactaag gaaattcctt ctttgtatgc aaattcaagt
    25681 tgcttccaac gaaaaaccat gacaacgatc tgagcaaaaa tccatataaa cacacctatc
    25741 ggatttccat cagttgccat attcgccgcc aaagccccaa aaatcgttgg aatcaaagca
    25801 gcaaatacgg aatctccaat cgctgcaaag gaacccatca atccagcttt cagccccgat
    25861 acggtttcct ttgaagcaat tccctctttt tcttccattg caagatcaat cccagtaatg
    25921 attgtattaa aaaaattaga agtattaaaa aattgcgtat gtgttttcat catctctttt
    25981 aattctggtg tattgtctcc atatattttt cttagttgtg gcaagatagt atataaatag
    26041 ccagaaccct gcattctctc atagttccaa cctaattgaa aagtgaacaa acttcttcga
    26101 ttgatttgct taaaatcttc ttttgttaat ttgtaattag atttcgtcat cttcgatttc
    26161 cccctcaaca gaatgtgatg gctcactagc taatggttga tggtctttgt tttgatcatt
    26221 cttataactg atcattgcta gtgctagacc aatcaatgct acagcaagca tcggcagcga
    26281 attgaaagtt gccggaagat ctttaacaac gcttgccaca cttgttccta ataattgaac
    26341 attagagaat aacgttgcta ataatgccgt gatcgtaaat cctaaaataa gataagatag
    26401 atgttttttt actggtaaat agcgcagaag aatggcaaac ccaacagctg gtaaaacagc
    26461 tcctgctact gaaagtccat cgcctagcca ttttagattc ccgtttaaaa cagaaacaac
    26521 attttcaact aatccaccac caaatgctaa agctaaaaat acaggaatca tacgagagag
    26581 cgaccaaggt aaagctccca ttaagaaatt gcgttcgatt gcttgataat tcatgtcttc
    26641 tacgtgacgg tcaatacgat gggcaaagta tgtgttagcg aatctagcta aaatatccaa
    26701 ttggatcatt aagcttgcta caggcactgc aatagcagca atcgcttgtt ctggattcat
    26761 tccaagagaa atcgagaagg cagttgctaa aatcgtccca gagttggcat ctatttttga
    26821 tgcgcctcca aacgtcccca ctcctaaaac tgtcaactgc atactgccac caataatcaa
    26881 acctgcgttt aaatcaccca ttactaaacc tgcatataac cctgcaaaca ctggcgcact
    26941 cattgaagaa tagatctgta attcatctaa aatttgataa cctgcatata atgttaataa
    27001 taagatctgc caccattgaa tatccatgga tcattcctcc tatttgatta aaagattcat
    27061 aaagtcttct gctttatcgt ttggtaccat ttgagcaatt aaatgtatac caagctgatc
    27121 taatttttta aatacttcga tatctgcatc taccacattg attgattttg taatggaccg
    27181 tgtttcattc gtttgtgaca tattccccac atttacttct ttgataggaa ctcctttttc
    27241 tactaaagat aagattcgat ctggcttttt tgctacgatc aatactcgtt gagagtcata
    27301 tttgccagct aaaatatttg ttgctgcttt ttcgattggc aaaatgctta attttacccc
    27361 tgctggagtc gctaacttca atccactttt ttcaattgtg tttgtggcta cctcatcatc
    27421 cacgaccatg attcgactaa tattcagttt cgttgtccaa agatttgcta cttatccatg
    27481 tatcaaacgt ccatcgattc ttacaccaat aattgccatc atgcattctc cttatctatt
    27541 ttttctattt attgttcaat taaaactgtg acatcatcaa atatcccttc tgtttcaaag
    27601 ataacgtttt cattttttct ttcttatatt tcatttttaa gctaagaaca aattccttcc
    27661 caactaaaga aaacgctttc ttttatattt aagttcattt gattttctta acttgaatgt
    27721 gtttcatcaa catcgtaact ttcatagggt cgatccagcc agttttctct tgttgcgcgt
    27781 ttgccatacc acaagccata gaaaatgcga gaatctcttc gattgattgc ctttttgata
    27841 acgcatgggc cgatcctgca agggtcgcgt cacctgaacc aacaggatta acaactgata
    27901 ttttaggaat aatgacttcg taaaaacact gagcaacttt tgccacagca cctctctctc
    27961 ctaaagaaac aacaacccat ggcactcctt caaatattgg atgagctaat aaggactgaa
    28021 ttttttctaa ttgaatcgaa gaacagatac gttggccaat cagttctcct aattcttcca
    28081 gattgggctt gataagtagt ggcttctcct taccttccaa tacttcaagc aaaaatttac
    28141 cagaagtatc tagtaatatg tctttccctt ttttatcagc taaagcaacc aattgcttat
    28201 agtaatcaag agggcatcct tggggtaaac tacctgagat cgtcataata tctgatattt
    28261 cgaggagctg ctcgaaatga tcggtaaatg tctgttgttc tttctttgtt atgaaggggc
    28321 ctcgttccaa aagctctgtt tgtctcgaac tatgcaggat agcaatagaa tcccttgtct
    28381 ctcctttaat tggaacaaac tgatgcatga tccctaactg atctagctgg ttctttagat
    28441 attcaccgtg ctggccacct attaaaccag tagctatcac ctcttctcct aataaattta
    28501 gaaccctcgc cacattcact ccttttcctc ccgctgtttt attgactgtt tcaactcgat
    28561 tgactttgtc gaggtttaat tcttcaattg tatacgcgat atcgatggaa ggattcaagg
    28621 taactgtcgt aatcatttca tcttcacctt tttgcttttt gttttattga accaaggggt
    28681 agcggtttca tttattactt tatttaaagt ctcgatgttt tctctcccat ttgttcttaa
    28741 ccaatgaata gctccgtctt ttccattttc gacgtatgct ttgaccccat ctttccatgt
    28801 cgcacgtcca cacaatacac cattgaaggt ggaacccgat tcatgagcaa aaatcagtgt
    28861 ttgttgaaat aattctgttg aaacaccccc actcaaaaaa atgaatggta atggagtcgc
    28921 ttcactttgc tccttaaaga aggcagcagc ctcttctttg ctgtgaataa attcacctgt
    28981 ggaatagcca ttcacataat tcatattgac tggtacttct actttcaaca catcaacttt
    29041 atattttgga tcagaaaatt ctttcatcat ctcaataact ttgtggggct tgactttcgc
    29101 atactctgga gaagaactat cgcttatttg tgcgtcataa gaaactaact ccaaatagaa
    29161 gggacgattt tctccttcgc actcactacc taaacgttcg atgaacacat gcttcaaatg
    29221 attgatttcg ggtgcttcat caacatcgta gtaaagtaag aattttactg catccgcttg
    29281 ctgttctttc aatctgagag aagaccaatc cgctaataaa tcaggtagac gaccaggcgt
    29341 agttgcgtca tagccggttt tctcataagc taacaataac cccgtctctt ttgctctgac
    29401 ttttgctgct ggcaatccat attccggatc caataaaata gcagaagcat agggtgtcag
    29461 ctcagctgat actagttctt tgaattcact gatttggtct tcacttggct cttttcccaa
    29521 cgatttgatc atttttttta gagcaccacg ctgatcgatc gctaatgcac taataatccc
    29581 atcttttgtt gatagtctct ccattgccct atttttttct tcattcatct gtcatttctc
    29641 cttcggtttc atacttatga ataatgacgc ccttgactac tcgattgacc gtgcctgttg
    29701 gagatggtgt atctggttta ttagctactt tgattgaagt atagagtgca atgatttgtg
    29761 cagtcataat catcgctaat gcaagatagg catcgggcaa tttactcacc gagtcttcca
    29821 atagcacagt catacctgag aaatcaatag tacccggttg cgcaatcgca atgacattgg
    29881 cagtgacccg atcttctttt atttcctcta aaatatctaa gtcataattt cgcgtatatg
    29941 gttgattgct cacaaaacct acaacgagtg tctcgctatt taaaaaagac tttggtccat
    30001 gacgaaaccc catcgaagaa tcaaaaattg tagcaacttt acctgctgtt aattctaaaa
    30061 tttttaattg tgcttctctt gtcgcacctg ccaaacttcc tgaacccaga taagcgatcc
    30121 gttcaaaaaa aacaggtaat aacttttgaa tttcttgatc tcttgaaaga agatcttgac
    30181 cgattttaac taattgttca agatatcttt cttttttctc cattgctgtt tgatcaaaga
    30241 tccataaagc actcaataac atacacgtaa aacttcctgt catagcaaaa cctgcatcgt
    30301 ttgctctctc tggcatcaat aacagataat tctgtttatc atcctgtgcg ttttttgcca
    30361 acatcccctc tttagcacaa gtaatcgtca aatgaaacaa tgtttcaact tgttcatttg
    30421 caatttgcac agccgctaca ctttctggac tgttgccact gcgagcaaat gaaactaata
    30481 aagtcggttc atttggaaac aaaaattctt ttggtgaagc tacaatatct gtcgtgccaa
    30541 aactgtggaa gataaaaaac tgattgtctc catgttcatt tagatagggt aagagcgtat
    30601 ccccaatata ttgagacgtt cctgcacccg taaaaataac acgtattctt cttccgtttg
    30661 cttctttttt tatttgatca aaaaatagag ctaaattttc ttggctttgc atcaatctat
    30721 cgattgtttc tttccagaga tctggttgtt gttttatttc ttgtgtcgtg attgctgcac
    30781 ctatttgttt taatttgttc ttcgatagct caaacatgtt gttctccctc tcaatcattt
    30841 tcggatatgc cgaattttat aatgaaactg gtccgctctt gctacactat ctgtatattc
    30901 gataatctca tttttcacgt tataggttat cctcgtcaga tgtaagacgg gagcgccatc
    30961 agcgattcgt agcacaacag catctttttt ttgcacgata cttgcataaa actcttcatc
    31021 agctaattga acttgttgtt cataatcctg tgaaaacaca tcatacaacg gtttactttc
    31081 aagtaacggc aaatcaagtc ccataaatag tttcgccggt agataagaac gctcaaccat
    31141 caatggctct tcatctgcca aacgcaatct tttaagttta aatacagttt cacctaaatg
    31201 gatgttcaaa ttcttagcaa ttctcttatt agcttccatt tgtctaaatt ctaaaatgac
    31261 cgtcttaggg acgcgcccta tttcgcgcat ttcttctgta aagctatatg ctccagctaa
    31321 gttcgatgac ttatcagaca aatcagagac ataggtacct tttccatgtc ttcggtaaat
    31381 ataaccttga ttttccaatt cctgtaaagc cagacgtacg gtggtacgac tcaccccata
    31441 gtgttgacaa agttctcttt cagataacag cttatcatgc ggagtcatct gtgtttcgat
    31501 acgatccttc aataaatcaa ctagttgata atacaatgat tttcctttat ttgagctctc
    31561 cataaaaatc acctcttctt tactggttat aaccacaaca caagaatagt gaattattaa
    31621 tcatttgtca aaggtttttt agaccaaaaa agctattata acagctcttt aaagctttat
    31681 atcttgaata gacaaagata cctcaaaaat accagttacc aataaaagaa cagaaaaaaa
    31741 gacgaaccac ataagcgatc cgtcaaaatg gtctaactta tcgagcagcg tgatccacat
    31801 taatccctat gatattccta caactatcgt tttattactt ccaatatcat ttacaacaac
    31861 taaaaatagc ttattaatga caaatgttat tattagtttt agataacttt tgcagtttta
    31921 tacttttttt ctataaaatt tgacaaacat ttttttaaat aactaattat tttttgaatc
    31981 tgtcactcag acaatccttt ctcttacttg ttaacataaa tagaagttgc tgtttcttct
    32041 aatagtgtac ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct
    32101 ttttcttcgc cttcggtaac aaaaggtgct actcccttcg cccatgttgc tcgcccacat
    32161 aagacaccat tgaatgtcga acctgattgt tttgcaaaac gcaatgtatc ttgaaatagt
    32221 gcagcgctga ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca
    32281 tagaaatacg cggatgcctc tgcttgacta taacaagtat cttgcccata tccttccaca
    32341 aatctcatat tcactggtac ttctactttc aatacatcca cttgataacg ttcttttgaa
    32401 aattctcgca tggcttcaat cactttatgc ggttttactt ttgcatagtc aagagaagac
    32461 ggatcgccaa tagttgcatc gtaagaaagg agctctaaaa agaaaggtat gccttctgct
    32521 tcgcattctg aaccaatccg ttctataact atttgttttt gcacattaat ctctactggt
    32581 tcatcaacgt catagtacaa taaaaattta caagcatccg cacccacttc ttttaacctt
    32641 ttcactgacc aactattcag taaatctggt agtcggcctg gcattttcgc atcatagccg
    32701 gttttttcat aagctagcaa caaaccacat tctttctctc gaagcgctgc tgcaggcaga
    32761 ccatattctg gatcgagtaa aatagcggaa gcatacggag tcaggtactg agaaaccatc
    32821 tgtttaaatt ggatgatatc ttctgatgta gcttctttat attggttgat cattttcttc
    32881 aaagcaccac gttggtcgat agctagcgcg ctaataattc cagcttcatc cgtcaatcgc
    32941 ttcaaacgtt cctgtttttc tatactcatt ttcttcatat atcttcacct atttctttag
    33001 ttaatattgt agctatttct atttttgatt ttgcagctct caactgttct gtaacctctt
    33061 ttcgcatcaa tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg
    33121 aatctgggat aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt
    33181 caattccttt tttcagtgtg acgataatca aactaggttc attgattgtt tgagacttcg
    33241 catgagggat agcaaaacct gacattattc ctgtagtacc ttctttttct ctttccagaa
    33301 gtttttgata tacttctttt tcgtcatgag cgattcctaa acagaatgtt tgctttgcta
    33361 aaaacttaaa tgcttcttct tgattatcta atggctgttg taaaaaaatg ttctcttcat
    33421 gaatgattgt catgataaac ctcctgctct tccttcttcg ataactgtac gatttgttgc
    33481 tttattatcc ctcttcttgt ctttatcagt tactcttttc ttttggatca aacctagtat
    33541 cactccacca acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac
    33601 tacaggtaac actaaaaaac cgccatgtgg ggctggtact tggacaccaa acagataggt
    33661 caagacagca gcaattgatg agcctaacat catcgttggt aatactcgga tcgggtcttt
    33721 cgcagcaaac ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa
    33781 acctgcatta cgatcattag tatcaaaata tttaccaaac aataaagtag caaaaccagt
    33841 aatcaacggt ggtgcaatgc aagctgctga cgcaccagcc ataaaagtcg tgtttccttg
    33901 tgtcaaaaga gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa
    33961 cgcacacata cagccaacaa tcacgccaag aacaagtgga ttcgaatatt caaatccagc
    34021 taaaaagctc atcattcctt catttatagc cttcattgga cctgccagca aagacatcac
    34081 gatgccaata aatgctacac caataactgg atataagacg attgccttca agccatctag
    34141 ttgtttaggt aaaattatca gtagcttttg caaaaataag ataaaataac ctgctaagaa
    34201 cccagctaaa atacctccta aaaatccagt accgccattc atggctacga ttccaccaac
    34261 aaatccaacg actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac
    34321 tggaaccatc attcccattg ctgcatcgtc aactgctttt accattgccg ctgtagcatt
    34381 gtattgtgca tgatctggat cagaagaata gattccccaa acggcaaatg aaatagcaat
    34441 caatactccg ccagctacca cgaaaggcaa catatgagag acaccgttca tcaaattttt
    34501 atagatgcta cgaccaaaat tagcaccact tatcgcttct ggttctaaag tatcagcagc
    34561 ttcattatct gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc
    34621 atccttgatt cctgtactga cagaaacatc aatgactcgt tttcccacaa atctttctga
    34681 atggacatct ttgtctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt
    34741 taattcattc tcgatcccaa tttgtccatg ggtctccact ttgatcgtga tgcctttttt
    34801 cctagctgct tgctctaatg cctcttgcgc catgtaggtg tgcgcaattc ctgtcggaca
    34861 accggttgct gcaattagtt gatattttcc catctttcga acctctcttc cttgattttg
    34921 atttgtttac ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcagta
    34981 gagctgcctg ctgctatgct tttacgtaac gtttcatcaa gcgatcgtcg attcatgtat
    35041 cctgctaaaa aagtgcctaa catggcatca cctgcacatg ctgtattgac aacttttcct
    35101 tttggcgagt ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg
    35161 agggataaca aaacattttc tgcgcctgac tctactagtc gttgtccata aacaaagtaa
    35221 tcttcatctg taaccatttt tcgtccgaac caagaagaaa gttcttcctc atttggtttc
    35281 agcaaaaacg gtcgataggg taaacaatcc atgacttctt gatcactact atcaataatc
    35341 aaacaaatcc gactgatttc tattaaaacc tttggagaca gccctcttgg taaactccca
    35401 gaaacacaaa gataatcccc tgcttgtaat gctcttattt gatttaaaaa attatgaaca
    35461 gctgtctgcg gtatttcagg tccttgattc actaatttgt attcttctcc cgtttgattg
    35521 acttgtgtaa atacattgat acgcgtcata ccaggcactt caataaactc tgtttggatt
    35581 tgtttctgct gtaaatagtc ttctatatac tttcctgtga acccagcaga aaaacctaat
    35641 gccgtacttg ggaaatctag catctttaat actagtgaca cattcacccc ttttccgttt
    35701 gcctgaatgt catcctcttt tgtgcggttt accataaaag gatgcatctc atctgtttca
    35761 ataaatagat caattgccaa attcatcgtg catgtgtaga tagacataac caacgacctc
    35821 cttgattcta ttatgaaata cgcgaaatag aaaatgtttt cttaatcgtc ccaacacaaa
    35881 gaaaaccttt acatttataa actcgtcgtg ctttttttaa ctaacttttg gtgaaagaag
    35941 gaaagaatat gaatcacaaa ttaagtgaaa cagagcagta cctctggaac tttatcgaac
    36001 acctcatcct agagatccct aactattcca tcgtgaaact aagtgaacaa gcaaatgtct
    36061 caacagccac cattgtgcgg acaatgaaaa agaaaggcta tgaagggttt acttcgttta
    36121 agcatcatct aaaagaagga gaaaacaaga acatcaattt ttcattttta gataaagtag
    36181 acaaaggaat ccgccgagcc attctaaaaa atgaacaaga agtggtacgt acaatcaata
    36241 tgttggaaat cgggaacatt gaagatgcaa tacaaagaat caaatttgct gaccgggttt
    36301 ttgtctttgc ccgaggattt tcagaaatga ttggccaaga aatgctggta aaacttcaat
    36361 taacgggaaa aaattgtcag atgcatactg atccagaaat tatcaaaagc atcagtcgga
    36421 aactaactaa gaaagacagc gttatttttg tttctttgaa
//
LOCUS       DACDOA010000017        35784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDOA010000017 DACDOA010000000
VERSION     DACDOA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.017"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="IWI39_000758"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="IWI39_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9207882.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="IWI39_000759"
     CDS             complement(1692..2711)
                     /locus_tag="IWI39_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207883.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="IWI39_000760"
     CDS             complement(2733..3740)
                     /locus_tag="IWI39_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9207884.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="IWI39_000761"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="IWI39_000761"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9207885.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="IWI39_000762"
     CDS             complement(4391..6685)
                     /locus_tag="IWI39_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9207886.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="IWI39_000763"
     CDS             6945..9110
                     /locus_tag="IWI39_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9207887.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="IWI39_000764"
     CDS             9414..10247
                     /locus_tag="IWI39_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9207888.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="IWI39_000765"
     CDS             complement(10349..12049)
                     /locus_tag="IWI39_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207889.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="IWI39_000766"
     CDS             12556..13716
                     /locus_tag="IWI39_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9207890.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="IWI39_000767"
     CDS             13732..14175
                     /locus_tag="IWI39_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207891.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="IWI39_000768"
     CDS             complement(14658..15188)
                     /locus_tag="IWI39_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9207892.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="IWI39_000769"
     CDS             15293..15844
                     /locus_tag="IWI39_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9207893.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="IWI39_000770"
     CDS             complement(15997..16470)
                     /locus_tag="IWI39_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9207894.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="IWI39_000771"
     CDS             16608..18080
                     /locus_tag="IWI39_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9207895.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="IWI39_000772"
     CDS             18077..18787
                     /locus_tag="IWI39_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9207896.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="IWI39_000773"
     CDS             19139..19423
                     /locus_tag="IWI39_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9207897.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="IWI39_000774"
     CDS             complement(19740..20123)
                     /locus_tag="IWI39_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9207898.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="IWI39_000775"
     CDS             complement(20125..20709)
                     /locus_tag="IWI39_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9207899.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="IWI39_000776"
     CDS             complement(20756..22231)
                     /locus_tag="IWI39_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9207900.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="IWI39_000777"
     CDS             22412..22723
                     /locus_tag="IWI39_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207901.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="IWI39_000778"
     CDS             complement(22838..25174)
                     /locus_tag="IWI39_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9207902.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="IWI39_000779"
     CDS             25316..25984
                     /locus_tag="IWI39_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9207903.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="IWI39_000780"
     CDS             25987..27030
                     /locus_tag="IWI39_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9207904.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="IWI39_000781"
     CDS             complement(27267..28148)
                     /locus_tag="IWI39_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9207905.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="IWI39_000782"
     CDS             28307..29035
                     /locus_tag="IWI39_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9207906.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="IWI39_000783"
     CDS             29191..29508
                     /locus_tag="IWI39_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9207907.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="IWI39_000784"
     CDS             29501..30109
                     /locus_tag="IWI39_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9207908.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30125..30955
                     /locus_tag="IWI39_000785"
     CDS             30125..30955
                     /locus_tag="IWI39_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9207909.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            31093..31581
                     /locus_tag="IWI39_000786"
     CDS             31093..31581
                     /locus_tag="IWI39_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9207910.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="IWI39_000787"
     CDS             31632..32504
                     /locus_tag="IWI39_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9207911.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="IWI39_000788"
     CDS             32680..33042
                     /locus_tag="IWI39_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9207912.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="IWI39_000789"
     CDS             33051..33803
                     /locus_tag="IWI39_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9207913.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="IWI39_000790"
     CDS             33817..35619
                     /locus_tag="IWI39_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9207914.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT        11052 a         6347 c         7376 g        11009 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttt
//
LOCUS       DACDOA010000018        34771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDOA010000018 DACDOA010000000
VERSION     DACDOA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.018"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(227..1114)
                     /locus_tag="IWI39_000791"
     CDS             complement(227..1114)
                     /locus_tag="IWI39_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9207915.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="IWI39_000792"
     CDS             complement(1111..1980)
                     /locus_tag="IWI39_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207916.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="IWI39_000793"
     CDS             complement(2117..3403)
                     /locus_tag="IWI39_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9207917.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="IWI39_000794"
     CDS             3676..4254
                     /locus_tag="IWI39_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9207918.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="IWI39_000795"
     CDS             4254..5183
                     /locus_tag="IWI39_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9207919.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="IWI39_000796"
     CDS             5180..6916
                     /locus_tag="IWI39_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9207920.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IWI39_000797"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IWI39_000797"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9207921.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="IWI39_000798"
     CDS             complement(7812..8321)
                     /locus_tag="IWI39_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9207922.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IWI39_000799"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IWI39_000799"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9207923.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="IWI39_000800"
     CDS             complement(9622..10713)
                     /locus_tag="IWI39_000800"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9207924.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IWI39_000801"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IWI39_000801"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9207925.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IWI39_000802"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IWI39_000802"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9207926.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IWI39_000803"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IWI39_000803"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9207927.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="IWI39_000804"
     CDS             complement(14676..15650)
                     /locus_tag="IWI39_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9207928.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="IWI39_000805"
     CDS             complement(15631..17514)
                     /locus_tag="IWI39_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9207929.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="IWI39_000806"
     CDS             complement(17532..19757)
                     /locus_tag="IWI39_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9207930.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="IWI39_000807"
     CDS             19871..20905
                     /locus_tag="IWI39_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9207931.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="IWI39_000808"
     CDS             complement(20972..21985)
                     /locus_tag="IWI39_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9207932.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="IWI39_000809"
     CDS             complement(22063..22251)
                     /locus_tag="IWI39_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9207933.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22691..22819)
                     /locus_tag="IWI39_000810"
     CDS             complement(22691..22819)
                     /locus_tag="IWI39_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9207934.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="IWI39_000811"
     CDS             complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="IWI39_000811"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9207935.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24306..25361)
                     /locus_tag="IWI39_000812"
     CDS             complement(24306..25361)
                     /locus_tag="IWI39_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9207936.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="IWI39_000813"
     CDS             complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="IWI39_000813"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9207937.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="IWI39_000814"
     CDS             complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="IWI39_000814"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9207938.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="IWI39_000815"
     CDS             complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="IWI39_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9207939.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="IWI39_000816"
     CDS             complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="IWI39_000816"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9207940.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="IWI39_000817"
     CDS             complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="IWI39_000817"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9207941.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33123..34682
                     /locus_tag="IWI39_000818"
     CDS             33123..34682
                     /locus_tag="IWI39_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9207942.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7495 c         6336 g        11422 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    22681 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    22741 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    22801 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    22861 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    22921 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    22981 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    23041 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    23101 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    23161 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    23221 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    23281 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    23341 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    23401 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    23461 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    23521 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    23581 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    23641 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    23701 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    23761 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    23821 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    23881 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    23941 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    24001 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    24061 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    24121 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    24181 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    24241 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    24301 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    24361 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    24421 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    24481 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    24541 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    24601 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    24661 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    24721 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    24781 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    24841 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    24901 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    24961 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    25021 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    25081 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    25141 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    25201 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    25261 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    25321 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    25381 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    25441 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    25501 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    25561 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    25621 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    25681 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    25741 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    25801 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    25861 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    25921 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    25981 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    26041 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    26101 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    26161 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    26221 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    26281 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    26341 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    26401 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    26461 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    26521 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    26581 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    26641 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    26701 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    26761 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    26821 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    26881 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    26941 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    27001 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    27061 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    27121 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    27181 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    27241 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    27301 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    27361 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    27421 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    27481 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    27541 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    27601 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    27661 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    27721 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    27781 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    27841 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    27901 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    27961 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    28021 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    28081 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    28141 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    28201 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    28261 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    28321 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    28381 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    28441 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    28501 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    28561 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    28621 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    28681 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    28741 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    28801 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    28861 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    28921 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    28981 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    29041 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    29101 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    29161 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    29221 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    29281 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    29341 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    29401 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    29461 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    29521 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    29581 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    29641 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    29701 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    29761 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    29821 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    29881 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    29941 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    30001 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    30061 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    30121 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    30181 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    30241 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    30301 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    30361 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    30421 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    30481 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    30541 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    30601 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    30661 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    30721 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    30781 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    30841 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    30901 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    30961 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    31021 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    31081 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    31141 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    31201 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    31261 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    31321 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    31381 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    31441 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    31501 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    31561 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    31621 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    31681 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    31741 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    31801 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    31861 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    31921 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    31981 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    32041 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    32101 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    32161 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    32221 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    32281 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    32341 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    32401 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    32461 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    32521 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    32581 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    32641 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    32701 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    32761 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    32821 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    32881 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    32941 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    33001 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    33061 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    33121 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    33181 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    33241 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    33301 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    33361 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    33421 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    33481 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    33541 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    33601 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    33661 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    33721 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    33781 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    33841 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    33901 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    33961 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    34021 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    34081 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    34141 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    34201 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    34261 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    34321 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    34381 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    34441 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    34501 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    34561 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    34621 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    34681 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    34741 attctataaa aaaagaagag gctgggaaaa c
//
LOCUS       DACDOA010000019        34658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDOA010000019 DACDOA010000000
VERSION     DACDOA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.019"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            153..1859
                     /locus_tag="IWI39_000819"
     CDS             153..1859
                     /locus_tag="IWI39_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9207943.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="IWI39_000820"
     CDS             complement(2017..2919)
                     /locus_tag="IWI39_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9207944.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3620)
                     /locus_tag="IWI39_000821"
     CDS             complement(2916..3620)
                     /locus_tag="IWI39_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9207945.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="IWI39_000822"
     CDS             complement(3651..4292)
                     /locus_tag="IWI39_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9207946.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="IWI39_000823"
     CDS             complement(4330..4644)
                     /locus_tag="IWI39_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207947.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /locus_tag="IWI39_000824"
     CDS             complement(4632..5261)
                     /locus_tag="IWI39_000824"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9207948.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="IWI39_000825"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="IWI39_000825"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9207949.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="IWI39_000826"
     CDS             complement(5981..6913)
                     /locus_tag="IWI39_000826"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9207950.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="IWI39_000827"
     CDS             complement(6910..7686)
                     /locus_tag="IWI39_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9207951.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="IWI39_000828"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="IWI39_000828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9207952.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /gene="carA"
                     /locus_tag="IWI39_000829"
     CDS             complement(10930..12006)
                     /gene="carA"
                     /locus_tag="IWI39_000829"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9207953.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="IWI39_000830"
     CDS             complement(12011..13291)
                     /locus_tag="IWI39_000830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9207954.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="IWI39_000831"
     CDS             complement(13310..14236)
                     /locus_tag="IWI39_000831"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9207955.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="IWI39_000832"
     CDS             complement(14249..15532)
                     /locus_tag="IWI39_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9207956.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="IWI39_000833"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="IWI39_000833"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9207957.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="IWI39_000834"
     CDS             complement(16320..17231)
                     /locus_tag="IWI39_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9207958.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /locus_tag="IWI39_000835"
     CDS             complement(17228..17692)
                     /locus_tag="IWI39_000835"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9207959.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="IWI39_000836"
     CDS             complement(17697..18395)
                     /locus_tag="IWI39_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9207960.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="IWI39_000837"
     CDS             complement(18382..18768)
                     /locus_tag="IWI39_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207961.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="IWI39_000838"
     CDS             complement(18915..20585)
                     /locus_tag="IWI39_000838"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9207962.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="IWI39_000839"
     CDS             20768..21001
                     /locus_tag="IWI39_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9207963.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="IWI39_000840"
     CDS             complement(21100..21531)
                     /locus_tag="IWI39_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9207964.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="IWI39_000841"
     CDS             21610..22023
                     /locus_tag="IWI39_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9207965.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="IWI39_000842"
     CDS             complement(22061..23209)
                     /locus_tag="IWI39_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9207966.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="IWI39_000843"
     CDS             complement(23331..23927)
                     /locus_tag="IWI39_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9207967.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="IWI39_000844"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="IWI39_000844"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9207968.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="IWI39_000845"
     CDS             27157..27588
                     /locus_tag="IWI39_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207969.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="IWI39_000846"
     CDS             complement(27633..28982)
                     /locus_tag="IWI39_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9207970.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="IWI39_000847"
     CDS             complement(29052..30215)
                     /locus_tag="IWI39_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207971.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="IWI39_000848"
     CDS             30344..30667
                     /locus_tag="IWI39_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207972.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="IWI39_000849"
     CDS             complement(30850..32199)
                     /locus_tag="IWI39_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9207973.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="IWI39_000850"
     CDS             complement(32268..33221)
                     /locus_tag="IWI39_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9207974.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="IWI39_000851"
     CDS             complement(33323..34513)
                     /locus_tag="IWI39_000851"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9207975.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9847 a         7591 c         6088 g        11132 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatc
//
LOCUS       DACDOA010000020        33415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDOA010000020 DACDOA010000000
VERSION     DACDOA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.020"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..374)
                     /locus_tag="IWI39_000852"
     CDS             complement(<1..374)
                     /locus_tag="IWI39_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9207976.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQT"
     gene            522..1811
                     /locus_tag="IWI39_000853"
     CDS             522..1811
                     /locus_tag="IWI39_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9207977.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1937..3016)
                     /locus_tag="IWI39_000854"
     CDS             complement(1937..3016)
                     /locus_tag="IWI39_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9207978.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3125..3565)
                     /locus_tag="IWI39_000855"
     CDS             complement(3125..3565)
                     /locus_tag="IWI39_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9207979.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3622..4032)
                     /locus_tag="IWI39_000856"
     CDS             complement(3622..4032)
                     /locus_tag="IWI39_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9207980.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4213..4647)
                     /locus_tag="IWI39_000857"
     CDS             complement(4213..4647)
                     /locus_tag="IWI39_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9207981.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4747..5595)
                     /locus_tag="IWI39_000858"
     CDS             complement(4747..5595)
                     /locus_tag="IWI39_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9207982.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5984..7750
                     /locus_tag="IWI39_000859"
     CDS             5984..7750
                     /locus_tag="IWI39_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9207983.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7852..9111
                     /locus_tag="IWI39_000860"
     CDS             7852..9111
                     /locus_tag="IWI39_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9207984.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9258..10559
                     /locus_tag="IWI39_000861"
     CDS             9258..10559
                     /locus_tag="IWI39_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207985.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10562..11413
                     /locus_tag="IWI39_000862"
     CDS             10562..11413
                     /locus_tag="IWI39_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9207986.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11426..12241
                     /locus_tag="IWI39_000863"
     CDS             11426..12241
                     /locus_tag="IWI39_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9207987.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12392..13558
                     /locus_tag="IWI39_000864"
     CDS             12392..13558
                     /locus_tag="IWI39_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9207988.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13788..14435
                     /locus_tag="IWI39_000865"
     CDS             13788..14435
                     /locus_tag="IWI39_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9207989.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14519..15463
                     /locus_tag="IWI39_000866"
     CDS             14519..15463
                     /locus_tag="IWI39_000866"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9207990.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15598..17049
                     /locus_tag="IWI39_000867"
     CDS             15598..17049
                     /locus_tag="IWI39_000867"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9207991.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17070..17906
                     /locus_tag="IWI39_000868"
     CDS             17070..17906
                     /locus_tag="IWI39_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9207992.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17906..18565
                     /locus_tag="IWI39_000869"
     CDS             17906..18565
                     /locus_tag="IWI39_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9207993.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18691..19380
                     /locus_tag="IWI39_000870"
     CDS             18691..19380
                     /locus_tag="IWI39_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9207994.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19386..20342
                     /locus_tag="IWI39_000871"
     CDS             19386..20342
                     /locus_tag="IWI39_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9207995.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20386..20898
                     /locus_tag="IWI39_000872"
     CDS             20386..20898
                     /locus_tag="IWI39_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9207996.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21154..21702)
                     /locus_tag="IWI39_000873"
     CDS             complement(21154..21702)
                     /locus_tag="IWI39_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9207997.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21791..22972)
                     /locus_tag="IWI39_000874"
     CDS             complement(21791..22972)
                     /locus_tag="IWI39_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9207998.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23208..24716)
                     /locus_tag="IWI39_000875"
     CDS             complement(23208..24716)
                     /locus_tag="IWI39_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9207999.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24875..25066
                     /locus_tag="IWI39_000876"
     CDS             24875..25066
                     /locus_tag="IWI39_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208000.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25269..26186
                     /locus_tag="IWI39_000877"
     CDS             25269..26186
                     /locus_tag="IWI39_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9208001.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26179..27252
                     /locus_tag="IWI39_000878"
     CDS             26179..27252
                     /locus_tag="IWI39_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208002.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27400..27918
                     /locus_tag="IWI39_000879"
     CDS             27400..27918
                     /locus_tag="IWI39_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208003.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27899..28291
                     /locus_tag="IWI39_000880"
     CDS             27899..28291
                     /locus_tag="IWI39_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9208004.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28367..29302
                     /locus_tag="IWI39_000881"
     CDS             28367..29302
                     /locus_tag="IWI39_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9208005.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29435..30214
                     /locus_tag="IWI39_000882"
     CDS             29435..30214
                     /locus_tag="IWI39_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9208006.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30207..30524
                     /locus_tag="IWI39_000883"
     CDS             30207..30524
                     /locus_tag="IWI39_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9208007.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30768..32378
                     /locus_tag="IWI39_000884"
     CDS             30768..32378
                     /locus_tag="IWI39_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9208008.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32535..33383
                     /locus_tag="IWI39_000885"
     CDS             32535..33383
                     /locus_tag="IWI39_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9208009.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11064 a         5967 c         6509 g         9875 t
ORIGIN      
        1 gtttgctgac ttaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc
       61 tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga
      121 tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt
      181 agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca
      241 gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg
      301 aaaagaaaaa tatctaattt tgtattgctt tttttcttca agagaaacgt tttaatcgct
      361 tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta
      421 ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat
      481 cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct
      541 taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag
      601 aggagataga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct
      661 ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag
      721 cgaccatcaa agcaaaaatt gactattcag gagacttagc acctatacta caatcagctg
      781 ttacatttga gaatgttgat cccgctttgt cgattggcta tgacgagaac actttgacta
      841 tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac
      901 cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat catttaacgc
      961 cgtatagtca gcggcagaca attgagcaag attcagctat cgactcagga ggagatcttg
     1021 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
     1081 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
     1141 aaccagagcc agacaatgaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtag
     1201 acccagaaga aaaaccggat gtgactccag aaccagatac ggattctgga aaccaaacag
     1261 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag
     1321 ccccagaaga aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag
     1381 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ctagaggtag
     1441 atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa
     1501 taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat
     1561 caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg
     1621 atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctagtcctg
     1681 aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga
     1741 gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca
     1801 gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc
     1861 agaaaacgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc
     1921 tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt
     1981 tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa
     2041 cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg
     2101 ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa
     2161 tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata
     2221 gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga
     2281 tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg
     2341 tgtttaaaag agcgataaca aattgagctt ctaataccaa accaaatgta ttgacaaact
     2401 tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc
     2461 ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca
     2521 tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc
     2581 cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa
     2641 caggatctgc atcttttggc tgatgctcgt aaaaatcaat aacggactgg atcatatcaa
     2701 atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga
     2761 tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga
     2821 tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca
     2881 tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt
     2941 tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa
     3001 taaacacgtt attcattata tgatggaata tgaaaataag aaagagaaag agcaaaaaga
     3061 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
     3121 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
     3181 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
     3241 cttctgcttg tttttgcaac gcagcacctt gaggagtcag cgtaacattt acgattcttt
     3301 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
     3361 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
     3421 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
     3481 ggagggaagt gtagtgtcta atgatttcct tagaggcggc atataaagga aaacatactt
     3541 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
     3601 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
     3661 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
     3721 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
     3781 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
     3841 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
     3901 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
     3961 acgatcggga ttatgaactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
     4021 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
     4081 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
     4141 cagacgcgct ttctcgaata gcaaacatat acaaaaaaat atcagagaca aaaactaatc
     4201 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aacttacttc
     4261 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
     4321 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
     4381 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
     4441 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
     4501 gccatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
     4561 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
     4621 aaaatctatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
     4681 ctagattctt ttattatacc agttaaagaa aacgcattca aaaaaatagc ggatttactt
     4741 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
     4801 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
     4861 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
     4921 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
     4981 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
     5041 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
     5101 aacaaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
     5161 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
     5221 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
     5281 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaacaa
     5341 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
     5401 cataacgata ttcaacacta aaataactat agctcggtca aatcctaaaa cagcttccag
     5461 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
     5521 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
     5581 tctcgtttct ttcatttatc cacttcctta attcattaat aatactctta tcttattata
     5641 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
     5701 tatgtttcat tcttctagaa tatacttcta cgttaaaaac tgttaaaaaa cttttacttt
     5761 agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt
     5821 agattaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt
     5881 cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg tttctctatc
     5941 acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat
     6001 ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg
     6061 gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctttgtggag atccatatac
     6121 cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa
     6181 tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca aggcgcttac agtatgcgtt
     6241 ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta
     6301 tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat
     6361 cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga
     6421 aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa
     6481 agatccagac cgagaagact tcttcggggg agatttgcaa ggtgtgaggg atcatttgga
     6541 ttacttgacg gaccttggcg tcaatggcat ctatttttgt cccatattta aagccacatc
     6601 caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa
     6661 acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc
     6721 tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga
     6781 aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat
     6841 gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt
     6901 catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac
     6961 ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga
     7021 tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat
     7081 ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt
     7141 gatgaattat gcattcacag attcgattaa agattatttt gcaaaaaaga aaatcacagc
     7201 ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga
     7261 aggaacattc aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg
     7321 taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg
     7381 tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg
     7441 tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt
     7501 ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt
     7561 atatgatcgt gaagataagc tctatttcac acgggaacta gagggacaga tcatctatgt
     7621 ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag
     7681 tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat
     7741 catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc
     7801 ccattataat aagggtgtaa cagcacgttg aatacacagg aggaaatgaa gatgaaaaaa
     7861 agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg
     7921 gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca
     7981 gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa
     8041 gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta
     8101 gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc
     8161 ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac
     8221 gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta
     8281 atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt
     8341 aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa
     8401 aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt
     8461 tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat
     8521 gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag
     8581 ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa
     8641 actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat
     8701 tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga
     8761 ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt
     8821 gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct
     8881 gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg
     8941 attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg
     9001 acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc
     9061 gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga
     9121 taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag
     9181 atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt
     9241 ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca
     9301 cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta
     9361 tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg
     9421 cattggtagg attggttact ttgggaagta taccaatgga agatcattca ttatttattt
     9481 tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga
     9541 atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga
     9601 cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc
     9661 ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg
     9721 catttactaa ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg
     9781 ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct
     9841 tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc
     9901 tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt
     9961 tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca
    10021 acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg
    10081 tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct
    10141 tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat
    10201 cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct
    10261 tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt
    10321 atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggt ggtcccggaa
    10381 gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga
    10441 ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg
    10501 ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac
    10561 aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta
    10621 cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac
    10681 tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt
    10741 aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt
    10801 gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac
    10861 ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca
    10921 gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct tattagaagc
    10981 gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac
    11041 ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact
    11101 tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat
    11161 gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa
    11221 gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc
    11281 tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc
    11341 gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg
    11401 aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt
    11461 acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt
    11521 gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca
    11581 gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg
    11641 aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac
    11701 gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga
    11761 tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc
    11821 tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac
    11881 gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat
    11941 cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt
    12001 attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac
    12061 attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc
    12121 agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac
    12181 tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg
    12241 acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt
    12301 tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg
    12361 taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt
    12421 attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca
    12481 atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat
    12541 gaaagagatt acaagtaacg cttctttggc actaggagaa gcctatatgg atcatcgaat
    12601 cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt
    12661 tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa
    12721 aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata aactttggct
    12781 agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca
    12841 agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac
    12901 gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa
    12961 tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat
    13021 aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa
    13081 aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa
    13141 tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat
    13201 ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt
    13261 ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt
    13321 gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac
    13381 aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg
    13441 aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt
    13501 gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt
    13561 gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga
    13621 agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat
    13681 attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg
    13741 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
    13801 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
    13861 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
    13921 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
    13981 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
    14041 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
    14101 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
    14161 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
    14221 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
    14281 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
    14341 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
    14401 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
    14461 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
    14521 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
    14581 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
    14641 tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga
    14701 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
    14761 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
    14821 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
    14881 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
    14941 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
    15001 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
    15061 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
    15121 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
    15181 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
    15241 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
    15301 acatctgcta tgtggcgcaa gcatgttaca gattggtaca gagcttcata aagaagggcc
    15361 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
    15421 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
    15481 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
    15541 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
    15601 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
    15661 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
    15721 cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca
    15781 ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct
    15841 acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg
    15901 caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc
    15961 aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt
    16021 acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta
    16081 gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt
    16141 gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa
    16201 ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat
    16261 gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca
    16321 catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag
    16381 attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg
    16441 catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat
    16501 cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga
    16561 gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag
    16621 ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg
    16681 gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct
    16741 aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat
    16801 tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc
    16861 acaccttata cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat
    16921 ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat
    16981 ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca
    17041 tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa
    17101 ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca
    17161 gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc
    17221 ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag
    17281 aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa
    17341 tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag
    17401 aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg
    17461 tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag
    17521 ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca
    17581 catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg
    17641 ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg
    17701 taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag
    17761 gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga
    17821 cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa
    17881 cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg
    17941 gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc
    18001 gtttctttta ggaaatttgg tagggttgct tttgacagtt ttaggtcttt tggattggct
    18061 gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt
    18121 cctgctgttt ctgttatatt tcgggttacc ttatcaatta agtgccttaa cggcttcagt
    18181 catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc
    18241 aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt
    18301 attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt
    18361 agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt
    18421 tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct atattttagt
    18481 tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa
    18541 acgttctatc catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt
    18601 tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt
    18661 aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa
    18721 tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg
    18781 cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga
    18841 gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg
    18901 tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa
    18961 actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag
    19021 atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta
    19081 ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca
    19141 aagcaaatgg agcgaggcgt aaaatatttt ccaaaagatc aattgtacaa agaattgaaa
    19201 agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag
    19261 gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat
    19321 tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga
    19381 aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag
    19441 cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt
    19501 ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga
    19561 tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag
    19621 tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag
    19681 tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag
    19741 aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct
    19801 ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc
    19861 atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc
    19921 acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata
    19981 aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata
    20041 tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt
    20101 ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga
    20161 ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg
    20221 gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc
    20281 atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat
    20341 gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa
    20401 acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt
    20461 gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg
    20521 aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca
    20581 gctgcattag tctcaggcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa
    20641 tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa
    20701 aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa
    20761 gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct
    20821 ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat
    20881 caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact
    20941 gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac
    21001 aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa
    21061 atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgatct
    21121 ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt tttttatttt
    21181 atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt
    21241 caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc
    21301 agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa
    21361 gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa
    21421 aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata
    21481 tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa
    21541 tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg
    21601 attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt
    21661 tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat
    21721 gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct
    21781 tttttgtatt ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg
    21841 ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc
    21901 ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga
    21961 cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca
    22021 tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt
    22081 ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt
    22141 ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa
    22201 aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc
    22261 ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt
    22321 cttctaatac atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa
    22381 caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca
    22441 tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg
    22501 gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc
    22561 cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt
    22621 tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata
    22681 caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa
    22741 aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata
    22801 cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta
    22861 aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa
    22921 aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac
    22981 tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg
    23041 aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa
    23101 caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat
    23161 cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat
    23221 agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt
    23281 tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct
    23341 agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc
    23401 aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct
    23461 tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa
    23521 tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg
    23581 gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga
    23641 ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca
    23701 ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc
    23761 aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag
    23821 gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca
    23881 gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca
    23941 ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg
    24001 aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc
    24061 agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct
    24121 gcatgtcctt tttggctttc atgatacagt tggttcaagc aagtagcgag ctttccactt
    24181 cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt
    24241 ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag
    24301 tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc
    24361 tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca
    24421 tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta
    24481 cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc
    24541 agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg
    24601 tcaactaact tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa
    24661 atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg
    24721 ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag
    24781 cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca
    24841 ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa
    24901 cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc
    24961 cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga
    25021 gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag
    25081 gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg
    25141 taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt
    25201 tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg
    25261 caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga
    25321 cgaacgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag
    25381 caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg
    25441 aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa
    25501 gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt
    25561 ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt
    25621 acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag
    25681 aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa
    25741 cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa
    25801 ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca
    25861 ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga
    25921 agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa
    25981 tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca
    26041 tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc
    26101 aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta
    26161 atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa
    26221 caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg
    26281 gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta
    26341 actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc
    26401 gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg
    26461 gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa
    26521 ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag
    26581 acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg
    26641 gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg
    26701 ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac
    26761 caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa
    26821 gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg
    26881 cagatcacga tattcaatca caaattgaat caattattag cccatgcaag aacacttggt
    26941 tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc
    27001 gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca
    27061 tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag
    27121 tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac
    27181 tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg
    27241 agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa
    27301 ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga
    27361 cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca
    27421 cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg
    27481 aatttccagc caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat
    27541 gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca
    27601 tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc
    27661 ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc
    27721 atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct
    27781 tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg
    27841 ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat
    27901 gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg
    27961 cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa
    28021 agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa
    28081 aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg
    28141 cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt
    28201 tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca
    28261 atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat
    28321 attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa
    28381 aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag
    28441 caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag
    28501 tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg
    28561 acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt
    28621 cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt
    28681 gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg
    28741 tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac
    28801 tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat
    28861 ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt
    28921 gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt
    28981 gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag
    29041 cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg
    29101 gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc
    29161 agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa
    29221 tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga
    29281 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
    29341 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
    29401 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
    29461 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
    29521 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
    29581 tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc
    29641 atttgcaacg acattattga tggttttctt tttagaatta cgctatacat tattaggagc
    29701 aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc
    29761 actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa
    29821 taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat
    29881 gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt
    29941 gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac
    30001 aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg
    30061 attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa
    30121 aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa
    30181 cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt
    30241 gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa
    30301 aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt
    30361 tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa
    30421 tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag
    30481 ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt
    30541 aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca
    30601 gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg
    30661 caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat
    30721 aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat
    30781 tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag
    30841 tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga
    30901 cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat
    30961 ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt
    31021 gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg
    31081 cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata
    31141 atgggatact agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg
    31201 caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta
    31261 tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga
    31321 ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt
    31381 tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag
    31441 attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca
    31501 ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg
    31561 accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac
    31621 tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct
    31681 ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag
    31741 atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc
    31801 ttccttcatt gactggaatg tctttattag ctggaccact atacacattg ttttttggct
    31861 atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt
    31921 tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat
    31981 taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg
    32041 gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct
    32101 atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct
    32161 taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc
    32221 tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag
    32281 gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt
    32341 tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg
    32401 tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta
    32461 gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg
    32521 aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac
    32581 gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa
    32641 tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta
    32701 aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat
    32761 tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct
    32821 ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg
    32881 actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg
    32941 atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct
    33001 atgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg
    33061 gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga
    33121 aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa
    33181 ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa
    33241 ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag
    33301 aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg
    33361 gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatat
//
LOCUS       DACDOA010000021        33254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDOA010000021 DACDOA010000000
VERSION     DACDOA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.021"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..230)
                     /locus_tag="IWI39_000886"
     CDS             complement(42..230)
                     /locus_tag="IWI39_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9208010.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="IWI39_000887"
     CDS             500..862
                     /locus_tag="IWI39_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9208011.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="IWI39_000888"
     CDS             914..2596
                     /locus_tag="IWI39_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9208012.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="IWI39_000889"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="IWI39_000889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9208013.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="IWI39_000890"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="IWI39_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9208014.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="IWI39_000891"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="IWI39_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9208015.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="IWI39_000892"
     CDS             6431..7435
                     /locus_tag="IWI39_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208016.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="IWI39_000893"
     CDS             7436..8380
                     /locus_tag="IWI39_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208017.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="IWI39_000894"
     CDS             8380..9342
                     /locus_tag="IWI39_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208018.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="IWI39_000895"
     CDS             9369..10283
                     /locus_tag="IWI39_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208019.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="IWI39_000896"
     CDS             10309..12090
                     /locus_tag="IWI39_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9208020.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="IWI39_000897"
     CDS             12438..13124
                     /locus_tag="IWI39_000897"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9208021.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="IWI39_000898"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="IWI39_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9208022.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="IWI39_000899"
     CDS             16734..17543
                     /locus_tag="IWI39_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9208023.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="IWI39_000900"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="IWI39_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9208024.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="IWI39_000901"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="IWI39_000901"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9208025.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="IWI39_000902"
     CDS             complement(21095..21547)
                     /locus_tag="IWI39_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9208026.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="IWI39_000903"
     CDS             21697..22647
                     /locus_tag="IWI39_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9208027.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="IWI39_000904"
     CDS             22640..23599
                     /locus_tag="IWI39_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9208028.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="IWI39_000905"
     CDS             23596..24351
                     /locus_tag="IWI39_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9208029.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="IWI39_000906"
     CDS             24374..25327
                     /locus_tag="IWI39_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9208030.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="IWI39_000907"
     CDS             complement(25971..26267)
                     /locus_tag="IWI39_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9208031.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="IWI39_000908"
     CDS             26622..26978
                     /locus_tag="IWI39_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9208032.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="IWI39_000909"
     CDS             26988..27887
                     /locus_tag="IWI39_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9208033.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28404..29135
                     /locus_tag="IWI39_000910"
     CDS             28404..29135
                     /locus_tag="IWI39_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9208034.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="IWI39_000911"
     CDS             29280..31307
                     /locus_tag="IWI39_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9208035.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIIIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="IWI39_000912"
     CDS             31415..32512
                     /locus_tag="IWI39_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9208036.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33254
                     /locus_tag="IWI39_000913"
     CDS             32819..>33254
                     /locus_tag="IWI39_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9208037.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT        11133 a         5762 c         7120 g         9239 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataatta ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaa
//
LOCUS       DACDOA010000022        32826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDOA010000022 DACDOA010000000
VERSION     DACDOA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.022"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(29..1261)
                     /locus_tag="IWI39_000914"
     CDS             complement(29..1261)
                     /locus_tag="IWI39_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9208038.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="IWI39_000915"
     CDS             1516..2085
                     /locus_tag="IWI39_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9208039.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="IWI39_000916"
     CDS             complement(2263..2703)
                     /locus_tag="IWI39_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9208040.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="IWI39_000917"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="IWI39_000917"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9208041.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="IWI39_000918"
     CDS             3657..4580
                     /locus_tag="IWI39_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9208042.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="IWI39_000919"
     CDS             complement(4805..5044)
                     /locus_tag="IWI39_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208043.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="IWI39_000920"
     CDS             complement(5049..5468)
                     /locus_tag="IWI39_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9208044.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="IWI39_000921"
     CDS             complement(5695..6933)
                     /locus_tag="IWI39_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208045.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="IWI39_000922"
     CDS             complement(7040..7675)
                     /locus_tag="IWI39_000922"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9208046.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="IWI39_000923"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="IWI39_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="IWI39_000924"
     CDS             complement(8017..8652)
                     /locus_tag="IWI39_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208047.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="IWI39_000925"
     CDS             9164..10306
                     /locus_tag="IWI39_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9208048.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="IWI39_000926"
     CDS             10299..10505
                     /locus_tag="IWI39_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9208049.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="IWI39_000927"
     CDS             10584..11777
                     /locus_tag="IWI39_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9208050.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(11784..11942)
                     /locus_tag="IWI39_000928"
                     /pseudo
     CDS             complement(11784..11942)
                     /locus_tag="IWI39_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            11982..13121
                     /locus_tag="IWI39_000929"
     CDS             11982..13121
                     /locus_tag="IWI39_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9208051.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="IWI39_000930"
     CDS             13114..13914
                     /locus_tag="IWI39_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9208052.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="IWI39_000931"
     CDS             13914..14741
                     /locus_tag="IWI39_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9208053.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="IWI39_000932"
     CDS             14719..15453
                     /locus_tag="IWI39_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9208054.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="IWI39_000933"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="IWI39_000933"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9208055.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="IWI39_000934"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="IWI39_000934"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9208056.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="IWI39_000935"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="IWI39_000935"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9208057.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="IWI39_000936"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="IWI39_000936"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9208058.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="IWI39_000937"
     CDS             19038..21071
                     /locus_tag="IWI39_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208059.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="IWI39_000938"
     CDS             21115..22395
                     /locus_tag="IWI39_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9208060.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="IWI39_000939"
     CDS             22605..23411
                     /locus_tag="IWI39_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208061.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="IWI39_000940"
     CDS             23423..24643
                     /locus_tag="IWI39_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208062.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="IWI39_000941"
     CDS             24633..26219
                     /locus_tag="IWI39_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9208063.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="IWI39_000942"
     CDS             26258..28396
                     /locus_tag="IWI39_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9208064.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="IWI39_000943"
     CDS             complement(28764..29747)
                     /locus_tag="IWI39_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9208065.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="IWI39_000944"
     CDS             30125..31516
                     /locus_tag="IWI39_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9208066.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="IWI39_000945"
     CDS             31532..32572
                     /locus_tag="IWI39_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9208067.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..>32826
                     /locus_tag="IWI39_000946"
     CDS             32591..>32826
                     /locus_tag="IWI39_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9208068.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNK"
BASE COUNT        10563 a         5696 c         6486 g        10081 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagtt
//
LOCUS       DACDOA010000023        32304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDOA010000023 DACDOA010000000
VERSION     DACDOA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.023"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWI39_000947"
     CDS             <1..276
                     /locus_tag="IWI39_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9208069.1"
                     /translation="YSKMGVCSLDTSFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYS
                     KMYMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(390..1019)
                     /locus_tag="IWI39_000948"
     CDS             complement(390..1019)
                     /locus_tag="IWI39_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9208070.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1153..1225)
                     /locus_tag="IWI39_000949"
     tRNA            complement(1153..1225)
                     /locus_tag="IWI39_000949"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1190..1192),aa:Thr,seq:ggt)
     gene            1439..1512
                     /locus_tag="IWI39_000950"
     tRNA            1439..1512
                     /locus_tag="IWI39_000950"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1473..1475,aa:Asn,seq:gtt)
     gene            1467..1616
                     /locus_tag="IWI39_000951"
     CDS             1467..1616
                     /locus_tag="IWI39_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208071.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2109..3437
                     /locus_tag="IWI39_000952"
     CDS             2109..3437
                     /locus_tag="IWI39_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9208072.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3639..4472
                     /locus_tag="IWI39_000953"
     CDS             3639..4472
                     /locus_tag="IWI39_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9208073.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4488..5225
                     /locus_tag="IWI39_000954"
     CDS             4488..5225
                     /locus_tag="IWI39_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9208074.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5380..6348)
                     /locus_tag="IWI39_000955"
     CDS             complement(5380..6348)
                     /locus_tag="IWI39_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9208075.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6580..7413
                     /locus_tag="IWI39_000956"
     CDS             6580..7413
                     /locus_tag="IWI39_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9208076.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7447..8286
                     /locus_tag="IWI39_000957"
     CDS             7447..8286
                     /locus_tag="IWI39_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9208077.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8312..8872
                     /locus_tag="IWI39_000958"
     CDS             8312..8872
                     /locus_tag="IWI39_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9208078.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9071..10792
                     /locus_tag="IWI39_000959"
     CDS             9071..10792
                     /locus_tag="IWI39_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9208079.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            10957..12099
                     /locus_tag="IWI39_000960"
     CDS             10957..12099
                     /locus_tag="IWI39_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9208080.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12115..13326
                     /gene="thiI"
                     /locus_tag="IWI39_000961"
     CDS             12115..13326
                     /gene="thiI"
                     /locus_tag="IWI39_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9208081.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            13899..14546
                     /locus_tag="IWI39_000962"
     CDS             13899..14546
                     /locus_tag="IWI39_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9208082.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            14939..17584
                     /locus_tag="IWI39_000963"
     CDS             14939..17584
                     /locus_tag="IWI39_000963"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9208083.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            17894..19207
                     /locus_tag="IWI39_000964"
     CDS             17894..19207
                     /locus_tag="IWI39_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9208084.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19197..19850
                     /locus_tag="IWI39_000965"
     CDS             19197..19850
                     /locus_tag="IWI39_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9208085.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            19905..20597
                     /locus_tag="IWI39_000966"
     CDS             19905..20597
                     /locus_tag="IWI39_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9208086.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20747..20947
                     /locus_tag="IWI39_000967"
     CDS             20747..20947
                     /locus_tag="IWI39_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9208087.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21201..22880)
                     /locus_tag="IWI39_000968"
     CDS             complement(21201..22880)
                     /locus_tag="IWI39_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9208088.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(22882..23094)
                     /locus_tag="IWI39_000969"
     CDS             complement(22882..23094)
                     /locus_tag="IWI39_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9208089.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23487..25217
                     /gene="efrA"
                     /locus_tag="IWI39_000970"
     CDS             23487..25217
                     /gene="efrA"
                     /locus_tag="IWI39_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9208090.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25214..26974
                     /gene="efrB"
                     /locus_tag="IWI39_000971"
     CDS             25214..26974
                     /gene="efrB"
                     /locus_tag="IWI39_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9208091.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27069..27266
                     /locus_tag="IWI39_000972"
     CDS             27069..27266
                     /locus_tag="IWI39_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208092.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27419..27904
                     /locus_tag="IWI39_000973"
     CDS             27419..27904
                     /locus_tag="IWI39_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9208093.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28008..28601)
                     /gene="clpP"
                     /locus_tag="IWI39_000974"
     CDS             complement(28008..28601)
                     /gene="clpP"
                     /locus_tag="IWI39_000974"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9208094.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            28781..29308
                     /locus_tag="IWI39_000975"
     CDS             28781..29308
                     /locus_tag="IWI39_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9208095.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29399..30136
                     /locus_tag="IWI39_000976"
     CDS             29399..30136
                     /locus_tag="IWI39_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9208096.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30140..31057
                     /gene="miaA"
                     /locus_tag="IWI39_000977"
     CDS             30140..31057
                     /gene="miaA"
                     /locus_tag="IWI39_000977"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9208097.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31059..32288
                     /gene="hflX"
                     /locus_tag="IWI39_000978"
     CDS             31059..32288
                     /gene="hflX"
                     /locus_tag="IWI39_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9208098.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10688 a         5498 c         6828 g         9290 t
ORIGIN      
        1 tattctaaaa tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa
       61 gaacagagaa aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata
      121 aaagattatt caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta
      181 ggtgtattta ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat
      241 ctacgaccag aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac
      301 tagtctcaat caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa
      361 gacgacacat tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct
      421 tgtgccatta tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt
      481 agccggccat tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct
      541 ttggtaccgc ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct
      601 aatgtttcaa gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca
      661 aatatcttag aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta
      721 ggagactcaa attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca
      781 agatagttta aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt
      841 atctgattca attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg
      901 ctgtttaaat gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag
      961 attacatctt tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata
     1021 ttgctctcct ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt
     1081 catttcttac gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc
     1141 actgtttaag gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc
     1201 gctctaccag ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat
     1261 taatgtattt gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat
     1321 tttagaaaaa ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa
     1381 attcaaaaaa agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc
     1441 cggcatagct cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc
     1501 ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct
     1561 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
     1621 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
     1681 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
     1741 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
     1801 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
     1861 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
     1921 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
     1981 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
     2041 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
     2101 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
     2161 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
     2221 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
     2281 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
     2341 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
     2401 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
     2461 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
     2521 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     2581 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     2641 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     2701 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     2761 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     2821 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     2881 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     2941 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     3001 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     3061 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     3121 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     3181 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     3241 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     3301 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     3361 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     3421 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     3481 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     3541 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     3601 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     3661 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     3721 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     3781 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     3841 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     3901 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     3961 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     4021 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     4081 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     4141 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     4201 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     4261 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     4321 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     4381 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     4441 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     4501 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     4561 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     4621 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     4681 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     4741 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     4801 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     4861 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     4921 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     4981 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     5041 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     5101 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     5161 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     5221 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     5281 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     5341 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     5401 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     5461 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     5521 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     5581 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     5641 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     5701 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     5761 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     5821 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     5881 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     5941 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     6001 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     6061 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     6121 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     6181 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     6241 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     6301 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     6361 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     6421 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     6481 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     6541 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     6601 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     6661 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     6721 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     6781 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     6841 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     6901 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     6961 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     7021 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     7081 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     7141 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     7201 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     7261 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     7321 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     7381 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     7441 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     7501 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     7561 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     7621 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     7681 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     7741 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     7801 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     7861 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     7921 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     7981 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     8041 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     8101 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     8161 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     8221 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     8281 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     8341 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     8401 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     8461 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     8521 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     8581 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     8641 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     8701 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     8761 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     8821 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     8881 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     8941 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     9001 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     9061 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     9121 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     9181 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     9241 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     9301 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     9361 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     9421 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     9481 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     9541 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     9601 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     9661 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     9721 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     9781 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     9841 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     9901 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     9961 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
    10021 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
    10081 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
    10141 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
    10201 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
    10261 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
    10321 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
    10381 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
    10441 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
    10501 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
    10561 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
    10621 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
    10681 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
    10741 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
    10801 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
    10861 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
    10921 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
    10981 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
    11041 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
    11101 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
    11161 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
    11221 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
    11281 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
    11341 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
    11401 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
    11461 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
    11521 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    11581 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    11641 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    11701 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    11761 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    11821 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    11881 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    11941 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    12001 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    12061 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    12121 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    12181 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    12241 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    12301 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    12361 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    12421 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    12481 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    12541 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    12601 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    12661 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    12721 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    12781 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    12841 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    12901 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    12961 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    13021 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    13081 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    13141 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    13201 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    13261 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    13321 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    13381 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    13441 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    13501 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    13561 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    13621 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    13681 agaggttgcg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    13741 ctcatatttt acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt
    13801 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
    13861 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
    13921 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
    13981 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
    14041 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
    14101 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
    14161 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
    14221 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
    14281 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
    14341 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
    14401 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
    14461 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
    14521 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
    14581 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
    14641 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
    14701 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
    14761 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
    14821 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
    14881 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
    14941 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
    15001 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
    15061 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
    15121 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
    15181 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
    15241 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
    15301 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
    15361 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
    15421 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
    15481 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
    15541 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
    15601 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
    15661 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
    15721 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
    15781 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
    15841 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
    15901 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
    15961 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
    16021 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
    16081 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
    16141 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
    16201 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
    16261 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
    16321 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
    16381 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
    16441 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
    16501 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
    16561 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
    16621 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
    16681 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
    16741 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
    16801 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
    16861 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
    16921 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
    16981 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
    17041 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
    17101 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
    17161 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
    17221 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
    17281 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
    17341 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
    17401 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
    17461 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
    17521 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
    17581 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
    17641 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
    17701 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
    17761 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
    17821 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
    17881 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
    17941 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
    18001 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
    18061 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
    18121 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
    18181 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
    18241 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
    18301 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
    18361 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
    18421 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
    18481 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
    18541 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
    18601 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
    18661 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
    18721 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
    18781 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
    18841 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
    18901 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
    18961 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
    19021 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
    19081 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
    19141 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
    19201 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
    19261 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
    19321 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
    19381 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
    19441 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
    19501 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
    19561 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
    19621 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
    19681 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
    19741 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
    19801 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
    19861 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
    19921 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
    19981 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
    20041 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
    20101 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
    20161 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
    20221 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
    20281 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
    20341 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
    20401 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
    20461 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
    20521 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
    20581 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
    20641 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
    20701 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
    20761 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
    20821 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
    20881 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
    20941 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
    21001 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
    21061 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
    21121 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
    21181 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
    21241 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
    21301 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
    21361 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
    21421 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
    21481 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
    21541 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
    21601 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
    21661 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
    21721 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
    21781 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
    21841 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
    21901 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
    21961 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
    22021 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
    22081 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
    22141 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
    22201 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
    22261 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
    22321 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
    22381 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
    22441 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
    22501 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
    22561 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
    22621 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
    22681 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
    22741 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
    22801 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
    22861 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
    22921 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
    22981 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
    23041 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
    23101 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
    23161 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
    23221 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
    23281 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
    23341 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
    23401 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
    23461 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
    23521 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
    23581 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
    23641 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
    23701 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
    23761 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    23821 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    23881 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    23941 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    24001 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    24061 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    24121 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    24181 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    24241 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    24301 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    24361 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    24421 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    24481 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    24541 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    24601 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    24661 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    24721 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    24781 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    24841 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    24901 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    24961 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    25021 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    25081 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    25141 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    25201 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    25261 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    25321 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    25381 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    25441 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    25501 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    25561 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    25621 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    25681 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    25741 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    25801 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    25861 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    25921 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    25981 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    26041 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    26101 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    26161 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    26221 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    26281 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    26341 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    26401 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    26461 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    26521 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    26581 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    26641 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    26701 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    26761 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    26821 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    26881 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    26941 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    27001 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    27061 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    27121 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    27181 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    27241 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    27301 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    27361 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    27421 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    27481 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    27541 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    27601 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    27661 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    27721 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    27781 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    27841 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    27901 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    27961 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    28021 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    28081 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    28141 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    28201 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    28261 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    28321 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    28381 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    28441 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    28501 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    28561 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    28621 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    28681 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    28741 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    28801 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    28861 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    28921 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    28981 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    29041 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    29101 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    29161 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    29221 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    29281 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    29341 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    29401 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    29461 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    29521 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    29581 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    29641 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    29701 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    29761 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    29821 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    29881 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    29941 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    30001 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    30061 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    30121 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    30181 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    30241 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    30301 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    30361 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    30421 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    30481 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    30541 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    30601 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    30661 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    30721 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    30781 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    30841 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    30901 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    30961 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    31021 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    31081 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    31141 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    31201 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    31261 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    31321 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    31381 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    31441 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    31501 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    31561 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    31621 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    31681 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    31741 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    31801 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    31861 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    31921 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    31981 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    32041 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    32101 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    32161 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    32221 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    32281 accaataaaa ttttattttt ttaa
//
LOCUS       DACDOA010000024        31898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDOA010000024 DACDOA010000000
VERSION     DACDOA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.024"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..602)
                     /locus_tag="IWI39_000979"
     CDS             complement(27..602)
                     /locus_tag="IWI39_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9208099.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="IWI39_000980"
     CDS             complement(607..1581)
                     /locus_tag="IWI39_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP9208100.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="IWI39_000981"
     CDS             1864..2832
                     /locus_tag="IWI39_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9208101.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="IWI39_000982"
     CDS             2929..3222
                     /locus_tag="IWI39_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9208102.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="IWI39_000983"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="IWI39_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9208103.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3688..4434
                     /locus_tag="IWI39_000984"
     CDS             3688..4434
                     /locus_tag="IWI39_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9208104.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="IWI39_000985"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="IWI39_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9208105.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="IWI39_000986"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="IWI39_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9208106.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="IWI39_000987"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="IWI39_000987"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9208107.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="IWI39_000988"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="IWI39_000988"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9208108.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="IWI39_000989"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="IWI39_000989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9208109.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="IWI39_000990"
     CDS             9171..9713
                     /locus_tag="IWI39_000990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9208110.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /locus_tag="IWI39_000991"
     CDS             9736..11292
                     /locus_tag="IWI39_000991"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9208111.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="IWI39_000992"
     CDS             11306..12208
                     /locus_tag="IWI39_000992"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9208112.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="IWI39_000993"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="IWI39_000993"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9208113.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="IWI39_000994"
     CDS             13651..14073
                     /locus_tag="IWI39_000994"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9208114.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="IWI39_000995"
     CDS             14542..14772
                     /locus_tag="IWI39_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9208115.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="IWI39_000996"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="IWI39_000996"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9208116.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="IWI39_000997"
     CDS             16183..16359
                     /locus_tag="IWI39_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9208117.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="IWI39_000998"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="IWI39_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9208118.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="IWI39_000999"
     CDS             16854..17321
                     /locus_tag="IWI39_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9208119.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="IWI39_001000"
     CDS             17406..18290
                     /locus_tag="IWI39_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9208120.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18407..18640
                     /locus_tag="IWI39_001001"
     CDS             18407..18640
                     /locus_tag="IWI39_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9208121.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="IWI39_001002"
     tRNA            18728..18808
                     /locus_tag="IWI39_001002"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="IWI39_001003"
     tRNA            18815..18886
                     /locus_tag="IWI39_001003"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19402..20109
                     /locus_tag="IWI39_001004"
     CDS             19402..20109
                     /locus_tag="IWI39_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9208122.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="IWI39_001005"
     CDS             complement(20168..20812)
                     /locus_tag="IWI39_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9208123.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="IWI39_001006"
     CDS             20939..21226
                     /locus_tag="IWI39_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208124.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="IWI39_001007"
     CDS             21334..21654
                     /locus_tag="IWI39_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9208125.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21972..23048
                     /locus_tag="IWI39_001008"
     CDS             21972..23048
                     /locus_tag="IWI39_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9208126.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23045..23764
                     /locus_tag="IWI39_001009"
     CDS             23045..23764
                     /locus_tag="IWI39_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9208127.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23892..24443
                     /gene="raiA"
                     /locus_tag="IWI39_001010"
     CDS             23892..24443
                     /gene="raiA"
                     /locus_tag="IWI39_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9208128.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24665..27199
                     /gene="secA"
                     /locus_tag="IWI39_001011"
     CDS             24665..27199
                     /gene="secA"
                     /locus_tag="IWI39_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9208129.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27325..28426
                     /gene="prfB"
                     /locus_tag="IWI39_001012"
     CDS             join(27325..27387,27389..28426)
                     /gene="prfB"
                     /locus_tag="IWI39_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9208130.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28531..29217
                     /gene="ftsE"
                     /locus_tag="IWI39_001013"
     CDS             28531..29217
                     /gene="ftsE"
                     /locus_tag="IWI39_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9208131.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29201..30094
                     /locus_tag="IWI39_001014"
     CDS             29201..30094
                     /locus_tag="IWI39_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208132.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            30246..31694
                     /gene="cls"
                     /locus_tag="IWI39_001015"
     CDS             30246..31694
                     /gene="cls"
                     /locus_tag="IWI39_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9208133.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10608 a         5565 c         6908 g         8817 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaaatattga ttattgacga tgtctataca acaggacaga cgctttttca
    23701 tgcagcagat tgcttgcaga acgcacagcc gaaaatcatc cgaaccattt ctttggcaag
    23761 atgaatcaga tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat
    23821 aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag
    23881 gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga
    23941 ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg
    24001 atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa
    24061 cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa
    24121 gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta
    24181 atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tgctgcttta ttcaatgaag
    24241 aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga
    24301 aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta
    24361 tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat
    24421 acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag
    24481 ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata
    24541 aattatgaag ttcttacttt aatacgctta agatatttca attatctata actaatgata
    24601 aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca
    24661 acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt
    24721 agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga
    24781 acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga
    24841 tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattaggttt
    24901 atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga
    24961 aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct
    25021 gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac
    25081 ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa
    25141 atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga
    25201 attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg
    25261 tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac
    25321 gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa
    25381 cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat
    25441 cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata
    25501 cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat
    25561 catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt
    25621 cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc
    25681 taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa
    25741 ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga
    25801 agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc gtccggttat
    25861 ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc acgctgtcgt
    25921 gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt
    25981 tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct
    26041 gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc
    26101 tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt
    26161 aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga
    26221 taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac agttctactt
    26281 gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaag ctttcttaga
    26341 tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt
    26401 agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca
    26461 atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat
    26521 catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg
    26581 tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga
    26641 ttttgccggc agtactcttg tccatgaaga tagcattact cttgcagata tcgaaggaaa
    26701 aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa
    26761 agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg
    26821 tgttgtggac tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat
    26881 cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa
    26941 gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc
    27001 tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga
    27061 agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa
    27121 taaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca
    27181 tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag
    27241 aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa
    27301 agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa
    27361 atgcaggaaa aggtcacaag tttcaggagg tctctttgac ttagatcagc tggaagaaga
    27421 catcgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgaca gcgaagccgc
    27481 acaaaaagtg atcagcgaaa ataacgctaa taaagaaacc tatgaccaat tccattctct
    27541 tgccgaagaa ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag aacctgatgc
    27601 agcaatgcaa gaagaagcag aaacacgtat caatgcatta gatgaaaaaa tgaagacgta
    27661 tgaattatct atgctgttag acggacctta tgatcgaaac aatgcgatcg tcgaattgca
    27721 tccaggggct ggcggaacgg agtcccaaga ctggggcagc atgcttttac gtatgtatac
    27781 acgttgggca gagcagcatg gctatcaagt agaaacacta gattatcaag caggtgatga
    27841 agcagggatc aagagtgtga ccctgctgat taaaggacat aacgcttttg gttatctaaa
    27901 atcagaaaaa ggtgttcatc gccttgtacg gatttcgcct tttgactcag cgaaacgacg
    27961 tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga
    28021 aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca
    28081 catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc
    28141 tagtcaggca caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc
    28201 taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga
    28261 acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc cctattctat
    28321 ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga
    28381 ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac ccattgttaa
    28441 attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct
    28501 aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag
    28561 tactcaaatg gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca aggagagttt
    28621 gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga
    28681 gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa
    28741 aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta
    28801 ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa
    28861 cctcgagata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa
    28921 gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg
    28981 gcaatcgtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca
    29041 gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc
    29101 gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc
    29161 gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac
    29221 ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt
    29281 tgcttcagtc agtgcggtaa cgatcacgtt gactttagtc ggtgtattca tggcagttat
    29341 catgaatgca actaaattag ctcaggatat agaaggaaac gttgatgtat ctgtattcgt
    29401 agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga
    29461 tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga
    29521 aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt
    29581 agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa
    29641 gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg
    29701 tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct
    29761 catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg
    29821 tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat
    29881 cggtctgatc ggtgcaattc ttccaatttt gatcatcaca tttggttatg catggttttt
    29941 ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc cacaaaatat
    30001 cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc
    30061 gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata aaatgagaaa
    30121 gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc
    30181 ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg
    30241 aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc
    30301 tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatggctgc
    30361 tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga
    30421 tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa
    30481 ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag
    30541 tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta
    30601 aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata
    30661 tcaagcaggc agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag
    30721 gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc
    30781 ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag
    30841 caggcggaga agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca
    30901 attatcgcaa tcatcgtaaa atcgtagtta ttgatgggga aatcggatat acaggtgggt
    30961 tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc
    31021 atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga
    31081 attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc attcacacta
    31141 ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca
    31201 aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt
    31261 attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa
    31321 aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt
    31381 ctttcgctgc agagctgatc gagtatggag caaggatcga aacatatgag aatggattta
    31441 ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg
    31501 atgttcgttc atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg
    31561 cttcacgtgt aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag
    31621 aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt
    31681 cgccgctcct ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt
    31741 ccttttttaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga
    31801 gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata
    31861 tgctattaaa agagacacat ttcaaagcca gtatagca
//
LOCUS       DACDOA010000025        30878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDOA010000025 DACDOA010000000
VERSION     DACDOA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.025"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(269..2344)
                     /locus_tag="IWI39_001016"
     CDS             complement(269..2344)
                     /locus_tag="IWI39_001016"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9208134.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2346..3263)
                     /gene="glyQ"
                     /locus_tag="IWI39_001017"
     CDS             complement(2346..3263)
                     /gene="glyQ"
                     /locus_tag="IWI39_001017"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9208135.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3647..4459)
                     /gene="recO"
                     /locus_tag="IWI39_001018"
     CDS             complement(3647..4459)
                     /gene="recO"
                     /locus_tag="IWI39_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9208136.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4602..5501)
                     /locus_tag="IWI39_001019"
     CDS             complement(4602..5501)
                     /locus_tag="IWI39_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9208137.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5516..5917)
                     /locus_tag="IWI39_001020"
     CDS             complement(5516..5917)
                     /locus_tag="IWI39_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9208138.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5895..6371)
                     /gene="ybeY"
                     /locus_tag="IWI39_001021"
     CDS             complement(5895..6371)
                     /gene="ybeY"
                     /locus_tag="IWI39_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9208139.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6388..8580)
                     /locus_tag="IWI39_001022"
     CDS             complement(6388..8580)
                     /locus_tag="IWI39_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9208140.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8604..9575)
                     /locus_tag="IWI39_001023"
     CDS             complement(8604..9575)
                     /locus_tag="IWI39_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9208141.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10281..11660
                     /gene="brnQ"
                     /locus_tag="IWI39_001024"
     CDS             10281..11660
                     /gene="brnQ"
                     /locus_tag="IWI39_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9208142.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11816..12262)
                     /locus_tag="IWI39_001025"
     CDS             complement(11816..12262)
                     /locus_tag="IWI39_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9208143.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12289..12465)
                     /locus_tag="IWI39_001026"
     CDS             complement(12289..12465)
                     /locus_tag="IWI39_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9208144.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12627..13055)
                     /locus_tag="IWI39_001027"
     CDS             complement(12627..13055)
                     /locus_tag="IWI39_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9208145.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13147..14007
                     /locus_tag="IWI39_001028"
     CDS             13147..14007
                     /locus_tag="IWI39_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9208146.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14091..14384)
                     /locus_tag="IWI39_001029"
     CDS             complement(14091..14384)
                     /locus_tag="IWI39_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9208147.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14457..16184)
                     /locus_tag="IWI39_001030"
     CDS             complement(14457..16184)
                     /locus_tag="IWI39_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9208148.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16397..17323)
                     /gene="trxB"
                     /locus_tag="IWI39_001031"
     CDS             complement(16397..17323)
                     /gene="trxB"
                     /locus_tag="IWI39_001031"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9208149.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17468..18901)
                     /gene="ascB"
                     /locus_tag="IWI39_001032"
     CDS             complement(17468..18901)
                     /gene="ascB"
                     /locus_tag="IWI39_001032"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9208150.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18901..20304)
                     /locus_tag="IWI39_001033"
     CDS             complement(18901..20304)
                     /locus_tag="IWI39_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9208151.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20534..21436
                     /locus_tag="IWI39_001034"
     CDS             20534..21436
                     /locus_tag="IWI39_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9208152.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21477..23363)
                     /locus_tag="IWI39_001035"
     CDS             complement(21477..23363)
                     /locus_tag="IWI39_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9208153.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23460..24311)
                     /locus_tag="IWI39_001036"
     CDS             complement(23460..24311)
                     /locus_tag="IWI39_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9208154.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24769..26553)
                     /locus_tag="IWI39_001037"
     CDS             complement(24769..26553)
                     /locus_tag="IWI39_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208155.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26553..28304)
                     /locus_tag="IWI39_001038"
     CDS             complement(26553..28304)
                     /locus_tag="IWI39_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9208156.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28446..28670)
                     /locus_tag="IWI39_001039"
     CDS             complement(28446..28670)
                     /locus_tag="IWI39_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9208157.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28921..30507
                     /locus_tag="IWI39_001040"
     CDS             28921..30507
                     /locus_tag="IWI39_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9208158.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8625 a         6633 c         5506 g        10114 t
ORIGIN      
        1 gttgatttct aaattgtcat atattattaa aaatttttgc catttaaaaa gaaaagcgac
       61 agtttaccaa taaatttcaa ctgaattatc taaaagtaga tcagttagtt tagataattc
      121 agttgaaatt gaccgactca aattacttgt ataataccta ggaaaaaatt aactagcacc
      181 acgggtatag ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct
      241 attctctggt ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta
      301 gcgacaaagc catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg
      361 gttcttctgc catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta
      421 caagctgatc ataattttcg ttgatcgttt ggtcagcaaa gctttttttc attgcctcta
      481 ctgctttgtg cagttctttc tccgcttcat tttcgaacag ttcagggtct acttctgatt
      541 gtttttcaag ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg
      601 gtttgaagtc ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg
      661 tcagatcttt ttgttctgca ttcaaaacag cttcgatgac atcatgacga atattcttcg
      721 ttgtcaataa ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgcccttctg
      781 ataaaccaat gccgaaacga tccgtatctt tactgataac atcactaatc gtttcttgca
      841 gaacagacaa aggaaaggcc cattttttcg attcgatgat ccgaacgaca ccgtatgctt
      901 ggcgacgtaa ggcataagga tcgtttgacc cgcttggaat catcccaact gcaaagaaag
      961 aaaaaatgct atctaattta tctgcaatcg ctaaaacaga acctactgta gactgaggta
     1021 gctctccttc actggatata ggcatgtaat gctcacgaat cgctgttgct acagcaggtt
     1081 tctcgccttt cagcagagca tatttttcgc ccatgatccc ttgtagttca ggaaattccc
     1141 ctaccatgtt ggttactaga tcaaatttat agatttcagc agctcgttgc agatccatca
     1201 attcttcctc agatagtcct acttgttcgc caatgatacg ggcaatgact tgtacccgct
     1261 gcattttctc atagatacta ccgatttttt catggaaagt aacagttttt agtttttcaa
     1321 cgtattggtc gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac
     1381 gagccgtcaa tactttttga ttgcctcgaa ccacattttc caattttacc cgatcaccat
     1441 tacgagcaga aataaaatga ggaagcagca taccttgatc gttccgtacc tcgaaataac
     1501 gttgatgttc tttcatagaa gtgactagta cttcttcagg aacagaaaga tatttttctt
     1561 cgaagtcgcc agaaaagaca gttggatatt cgactaaatt gttgacttct tctagtaaat
     1621 cttcgtctag atcaataacc cagtgatttt tttcggctaa ttcatttgct tgtgtaacga
     1681 tcatttcttg tcttttttgc ggtgaagcaa taacgaattg ttcattcaat ttttcttcat
     1741 attcttttgc atgcgcaaat gtcacatctt cgcctaagaa acgatgtcct cgagaggctc
     1801 tgcctgtttt gacatctaag atctcaaacg ggattacttc gtcatctaac aatgccacga
     1861 tccagtgaat agggcggata tattcaaaat cgtattctgc ccaatgcatc gttactggga
     1921 aagtgaggct tttaatgaca tctgctaaac ctgtcaatac ttctttcgct tctttgccgt
     1981 gagtgtattt cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat
     2041 ctgtcgtcag accttgtccg cggacaaatc cttcagctgc tttcgtccag tttccatctg
     2101 cgtccaaagc aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt
     2161 ctggaatgtt tgttactttg attgctaagc ggcgcggtgt agaaaacgtt tcgatcgtat
     2221 cataagtcaa ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc
     2281 ttggtgtgac aacatgagca ggcatttctt ccagcccgat ttcaagtaat aagtcttttg
     2341 ccatcttatt cagcctcctt catgttttct ggatttgctg tgtttttatt caataaaggg
     2401 aatcctaatt tttcgcgttc tgccacaaag attttggcta cagcacgagc catgttgcgg
     2461 atacgggaaa gataacctgc tcgttctgtt acggatactg caccgcgagc atctaataaa
     2521 ttgaatgtat ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg
     2581 atacagcgtt tagcttcttt ttcaaaacgg tcaaagttgc ttagtagcaa gtcttggtca
     2641 cttacttcaa aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat
     2701 ttcacaccat cagcccattc cagatcatag acactttcaa cttcttgaat atatgaagca
     2761 agacgttcta gtccataagt gatctcagaa gtaactggtt tacaaggtag tcctccgact
     2821 tgctggaagt acgtgaattg tgtgatctcc atcccatcaa gccatacttc ccaacccaca
     2881 ccggcacacc ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct
     2941 aatggatcaa ttcctaaaag acgcaagctt tctaaataaa gctcttggat attttcaggt
     3001 gaaggtttca ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca
     3061 taacgcccgt cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt
     3121 ccgattgccc gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtgtcataa
     3181 gcttgcatca gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct
     3241 tggacggtca attttttact catctgtttt gcccctttca atttttaagc gttgctgtca
     3301 ataagaatac aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac
     3361 gaataatttc gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta
     3421 ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa
     3481 accacgtgtt ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt
     3541 atataataga tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt
     3601 ccctgtattt gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt
     3661 caggtatctc tttttcaggc atatttttct ctggcgtctt taatacgtgt gcccaagact
     3721 tcatttgatc aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact
     3781 ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct
     3841 tatcataaga aatagccgaa aacattcgaa cgaaataaac agcgcgagga tctgcatgat
     3901 aacggtgttc gtccattggc cagtgtttct cgcatatcac accgccagat ttggaagaat
     3961 agtcaaattt tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc
     4021 cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt
     4081 gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca tattgatgat
     4141 cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc aaaaagatat
     4201 cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt
     4261 cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg
     4321 gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa
     4381 agattttgac taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg
     4441 attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg
     4501 ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc ggatttttat
     4561 ccagtttatt tctatactta acctataaga agttcagaat atcagtattc gtcttttcgg
     4621 tagccaaagt cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa
     4681 agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct
     4741 acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct atctctttcg
     4801 acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca
     4861 acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc
     4921 aattcagaaa cgataaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt
     4981 gggccctctg gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct
     5041 gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt
     5101 cccagcaact cgtctggatg gatcttatcg attttattga taatcagata aactggagaa
     5161 tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca
     5221 ctgatcataa ataagactgc atccacttct ttcaacgcac tgtaagctgc ttctaccata
     5281 aaatctccta gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga
     5341 gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc
     5401 ataatggcaa ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg
     5461 cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat
     5521 taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac
     5581 aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa
     5641 aatgaaaatc cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta
     5701 tccaaacgag aaaaacagct aaaagcagcc ataaccactc tatccgatca agctggaaaa
     5761 tcacgcccat aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt
     5821 cctcaaaaac ggttttgact ccttggacag caaattccac agacatcagg aaatgtttgt
     5881 ttttctctac gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca
     5941 aacatttctt tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca
     6001 tgtaccgcta aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca
     6061 gcagcacgtt caatagaaat catgatgtct cctagtacac gaggcaaagg attctctcca
     6121 tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg
     6181 tctgtcggtg catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc
     6241 atgaaagtaa cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc
     6301 agcaagtcat cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag
     6361 gtaatatcca ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac
     6421 ttctgttttc attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat
     6481 caatgatttt tccacttttc ggatttcttt caaggtcagt ccactatcgt ctaattgacc
     6541 atcggaaata cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgtcgg
     6601 atgatccatt gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc
     6661 acgactttgc ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg
     6721 ttctttcgct ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat
     6781 atcgatcacc atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg
     6841 tccaaagata atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc
     6901 tgaaggcaag ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata
     6961 gcaagctact cgagttagca aagaacgtcc gccgatttct gctacagcgt tagcacttag
     7021 attagctacc atcatactat gatgataagt acctggcgct tcttccagca actgtttcaa
     7081 taaaggatga tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat
     7141 caattcaata tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa
     7201 cccgcagaca agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat
     7261 caaaatgaca tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg
     7321 ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat
     7381 caagataatc gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcagatac
     7441 tgcttggaaa ataccagcca tgatccctgc tcttcggttc aagaaaaagc ttaaaacaag
     7501 tggtgcaaaa gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa
     7561 ttggaaaaat ttcatcagca ctatgctgac tgacatagct gtcacataga aaagcacgta
     7621 ttttgttctt tctcctgcat tttggaattg aagtgaatta taaatcagta cgccgatttg
     7681 aagcaaaaca gctaaaacca tggcaatgag tgggaaaatc gaagtcgttt gattcgtcaa
     7741 tcctagtaat tccaattttt tcatcgctga agcatcaatt tgatttcctt cgcgaacaat
     7801 gatttctcct tggaagatca tcactggttg tacagcttct cgtgcagatt gttttaattc
     7861 ttctgtcttt ttctcattca atacatcatt gactacaatg ccttgatcca ctaagtagcg
     7921 aatcatttgt tgctgctctg ttgtgacatt caaatactga atctgatctt ctgcttcttg
     7981 tttaaaagct tccaattcgc tttcacgaac ttgtttactc atctgttcgt caatgagctt
     8041 caaactttca tccctaaccg tgtgaatttc cgtttcagtc atttcaaata tcgtcgaata
     8101 aaaattgttc ggcagttttt ggtaaaacgc aacgttttct gcattcacgt tttcaaaatt
     8161 tttcttcagt gtagcaatcc gctcttctgt agtaggtttc gggatatttt catcttcctt
     8221 cgccttatct accttgtcat tataagattt gttgatggta tcattcgtct tatcgatcaa
     8281 ttcgaataat tgcttgatcc gattgttctg gtcatctgcc agatcttttt gataagtata
     8341 ttctggagtc actgcttctg ctgccagttt tcttctttgt tcggtttctt ccgtgttctc
     8401 gatcgtttta ttcgcacgga tactctcttc tgctacttgg ccttctctga aatcattggc
     8461 cttttgtttg acactactaa atgtaacgcc gcagacaatg ataaaaaaca agaacagaag
     8521 gaaaggcaat gctgcctttc cgagtttttc ttgtagtttc tgcagtgttt tattcaacat
     8581 gtgcttatcc tcctaagcaa agcttattct tgatgaagat ccgccttttc ataagctttg
     8641 atgatttcgg caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg
     8701 atttttttga tttgctttag tgttcgttct gcatgaacaa gaccacttgt cgtccctcga
     8761 ggcaagtcta tctgacttgt atccccattg acaatcattt ttgattgata accgagacgg
     8821 gtcaagaaca ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa gataacgaat
     8881 gcatcatcca atgtccgccc ccgcatgtaa gctaatggtg cgatttcgat tacgcctcgt
     8941 tccatcaatc ggttcgtatg atccaatcca aaaatttggt ataaagcatc ataaacaggc
     9001 cgcagataag gatccacttt ttctttcaga tccccgggta gaaagcctag gttttcccct
     9061 gcctctacag cgggccgcgt caaaatgatt ttttgcactt cgcctttttt tagggcagca
     9121 attgctaata ctacggcaag aaacgttttc cctgttccgg ctggccctac accaaatacg
     9181 atgtcatgtt tggctaccga ttcaacgtat ttcttttgac cgctgttttt tacccgtata
     9241 ggttttccat tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat
     9301 gtaccttttt gccccatttt caatgccgtc acgacatctg gtgtacttac gtggattcct
     9361 cgtttgatca attcctgtaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt
     9421 tcaccaatga tctgaatgat ttctccacgt gtatggatgg ttgtttccgt tgtttcctcg
     9481 atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagctgtc
     9541 aatctaatct ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat
     9601 tcattctttc ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttgt
     9661 aaaagagtcg tttaaccata cgtaccaaga aacgttaaaa aatagctgtt tccattcgct
     9721 aaaggtctcg ttttgataaa aagaatctga gaatgtaagt tatatgaaag tggagaacaa
     9781 aatattccgt ttcattcaga ggtattctct aggtattttt cttgtttcaa aaaaagaaaa
     9841 caaaatagtt aaaatattct tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa
     9901 taaacataca gcaaagcgtc gaggaaaaca gtaggctatt caacgattta ttcccagtga
     9961 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
    10021 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcaaaa
    10081 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10141 gagccctttt cccaagtgga aaagggcttt tttttatatt ttaaggtggt ggttttcatg
    10201 gaatctgata ttagtggcat tactaattgc tgcaacgttt atatcgtcaa tcaaaatgat
    10261 aattattgga ggaaaaattt atggaagaaa caaaaactta ttcatggaaa caactattga
    10321 tggtcagttc acttatcttc gggatgtttt tcgggtcagg taatctaatc tttcctgtcc
    10381 acttaggtca aatggctggc gcaaactggg tcagtgctgg cgtaggattt gccatctcag
    10441 gcgctctttt cccattacta gctatcttag ctgtagtagt gacaaaaagt gatggtctgt
    10501 atgacttagc taaacctgtc ggaaaatggt atgctgcctt gttcttaatc cttgttcatt
    10561 tgacgatcgg accatttttc ggtacaccac ggacagctgc tactgctttt gaaatggctg
    10621 ctaaaccatt tttgcctgaa aaatataatt cattaggtat gtttttattc tctgctcttt
    10681 tctttttact tgcttatttc cttactgtcc atcccacacg gttggtcaaa tacgtcggaa
    10741 agtatctaaa ctccttgttt cttgcattat tagcaatcat ttttgtcgtt tctttattcc
    10801 acccaatggg aaatctaaat caatctgctg cagatactta ccaaacaaac gctttaacaa
    10861 atggtgtcct agaaggttat aacacggttg atgcagtagc cttgcttgcc ttaagtgtca
    10921 cttttgtcca tgcagtccgt gaccttggtt tcaaagataa aaaagtcaca gaattaacag
    10981 caaaagctgg tagcctgagc gttgcgctag aagtcatgat ctattttggt cttgttcttc
    11041 tcggtgcgat gagtttgaat aagatgtctt tatcagaaaa tggcggaact gcactttctc
    11101 agatcactaa tttttattta ggtaacttcg gcgctagctt cttaggagtg atggtcacat
    11161 taggtgtctt cactaccgct atgggactcg ttgtttcttt tgcacaagat ttccataaac
    11221 tgttccctaa agtcagttat atgacatggc tgcggttaac gactttcgtc tcttttgtcg
    11281 ttgcaaatgc tggtttggac aatatcatcc aatggtcttt accagtattg atgcttttgt
    11341 atccattgtc gcttgcgttg attttgcttt ccttaacagc aaaatttttc caaaagacac
    11401 catttgtcta tcaagtaacg atgttgtttg ctgctgttcc cgccgtattg gatatgctgg
    11461 caaattcgcc tgctttagtg agtcaacaaa gagttgtagc aagcatgctc gaattttatc
    11521 atcatcacgt tccttttgct gcattgggac ttggctggat ggtcccaacg ttacttgggt
    11581 atgctggcag cttactcttc tattacgcct atcgcctatc agggtataaa caagaagcaa
    11641 atgaactacc agaagaataa ccagatcaaa cacaatttaa cataagaaat attaaacagg
    11701 aaaaaaacag ctagatgaaa gaatcctatt ctttcgtcta gctatttttt agatctcttt
    11761 tttacgagca caaaaaaact gcggacaatg attgtccgca gtttaaatga taaaattaag
    11821 attgtaatag ttctttcaca atggcattta cttggtttcc atctgctttt cctttgactt
    11881 taggcatgat cgcacccatg actttgccaa attctttcat tgatgaagca ccagtttgcg
    11941 tgattgcttc ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc ggcatgtatt
    12001 tttcgactat cacaatttca ctttttactt tttcagctaa atcgtcacgt cctgcttctt
    12061 caaactcatg caaagaatca cgtcgctgtt tcatttcgcg agacaacacc gttaattctt
    12121 cttcttctgt caagtcgtga ccttctttaa tctgctcgtt ttgtatagat gacttgatca
    12181 ttcggatgac ttgtaaagat tccttatctt tcgctttcat cgctgttttc atgtcatcgt
    12241 ttaatctact tagtagtgac acactaccaa ctccaattca tcacagaact agaatttttt
    12301 gcgttttcta gctgcttcag attttttctt gcgtcttaca cttggttttt cgtagaattc
    12361 acgtttacga gattcttgta aagtacccgc ttttgacacg gaacgtttga agcgacgaag
    12421 agcatcgtca agtgattcgt ttttgcgaac gactgttttt gacatgttaa ttccctccct
    12481 ccgagcttaa aaagtaaccg taattgttat tgaaatatat ctcaaaaaac aagactgtcc
    12541 tgtaaatatt atatctaatg ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat
    12601 tatcaaatgt tattttattt aaatccttat tgacaatttt cacatcttcc gaaaatctcg
    12661 aatctatgcc cttcgatcgt acatccaccg agctgttctt cgaaataatt cattggacac
    12721 atatggattt ctttcgttgt tccgcacacc gtacagataa agtgatggtg atggccgatt
    12781 tccccttgac agcaatgaaa acgaaacttc atctctccat ttaaatcggt ttcttccagt
    12841 aaatcgattt ctgcaaaatc atggagattt cgataaatcg tatcgtaact tactcctttg
    12901 aattcctgat tcatatagtc aaaaacttct ttagcggata cgtagcgatt gcgcttcact
    12961 aaataagtta acaatgcttc tcttttttta gtatatttaa aaccgttatt tttcaatgtt
    13021 tctagtgatt gttcgatcaa cgtctgtttt ttcatgactt attcctccaa atcaaaatga
    13081 ttacgaagta cacattctag agtatggtat aataaacata aaaaaaatgc aagtgaggtt
    13141 ttatccatgt cttccgattc tacagaaatc gttacttttt ttgtcatctc cgactctgct
    13201 ggagaaactg cgacaaaact agcgcaagcc actatggccc aatatcctag cgttgagttc
    13261 aatttattcc gcagaacatt tgtgacagat gagaaaacat tgatgcaggc actgcaagat
    13321 gcactagaag aaaaagctct tgtcctgcac acattgatca atcaagaatt gatcgagatg
    13381 acgcgttctt tttgtgaaga acatcaatta tttagttttg atgtgatgac tccgccgata
    13441 tcagaaatcg agcgcttaac tggtatcaag cccattcggc aacctggcgc ccttcattta
    13501 ttgaatgaga actatttcaa acggatcaaa gcaatggaat ttgctgtaaa atacgatgac
    13561 gggaaagatc ctcgtgggtt tctagaagca gatgtcgttt tgctaggcgt atcccgaact
    13621 tccaaaacgc ctttgagctt gtttttagct aacaaaaatt taaaagttgc caatctacct
    13681 ttgatcccac aagcacatat tcccaaacag ctttgggaga tcgatcctaa aaaaatcgtg
    13741 ggactgacga ataacccaga tattttaaac aatatccgta aagaacggat gcgctcttac
    13801 ggattaaatc ctgatactgc ttattccgat atcgaaaaaa tacgagctga actcgatttt
    13861 gcgaatgatc tttatgaaaa attgggctgc gtagtaatca atgtcgcttc tttatctatt
    13921 gaagaaacag cttcgcttat tttgaatgct ttagacctag aagaccatag ttattatgga
    13981 actgaaacca ccgaagaaac agactaacaa tttttcttta aaaaaactgg actgtgtcag
    14041 cgaagaagat tcgttgacac agtccagttt gtctctattt gcacctatcg ttattgatgt
    14101 tccacatgtt tgagtgtctg gttcaaaata tccagtacgt gagaatcatc aagactatac
    14161 aaaatcgctt ttccctcacg atgggacttg acgacatggt tttctcttaa taaacgcagt
    14221 tgatgcgata cagcagattg ttccatttcc actgcttgga tgattgctgt cacattttgt
    14281 tccccgtctt tcagatacag cagaattttg agacgagtgg ggtcactcaa cattttgaat
    14341 agttggctga ctttctctac ttttgttttt tgcgtgtctg gcaatccatt cacctccaac
    14401 ttgaaaaaag actgacttct aaagtttacc actttttcca tcagtctcaa gtttttttat
    14461 tcttctgtca tcttttggat aatcgtttca taagcggcta ctttttcagc tgcgtcttct
    14521 gaagagtttc cttttacacc gatataaaat ttgattttcg gctcagttcc tgaaggacgg
    14581 atagcgatcc agccattttc ttccagcgta tacttcaata cgtcagatgg cggtgtcgtt
    14641 agtttttcta cgtttccttc acggtctgtt ctcgtcaact ctttgaagtc ttctgtttgg
    14701 atgacttttg ctccggcaaa ttctgttggt gcttcttcac ggaatttttc catcaaagca
    14761 gagattttcg cagaaccttc ttgtccgctc attgtcacag aaatcgtttt ttctgcgtag
    14821 tagccatagg tttcaaaaat cgtttgcaag ccatcataca acgttttgcc ttgtttttta
    14881 tagtaagctg ctacttctgc taataagagc aatgcttgga tcgcgtcttt gtcgcgaacg
    14941 aaaggtttaa ctaaataacc gtaactttct tcgaatccga acataaaagt atgggaatga
    15001 tcttcttcaa attgttgaat tttttcagca atgaatttga atcctgtcaa tacattgaac
    15061 atggtagccc catagtgttc cgcaattgct gtaggtaatt cgctagaaac gatcgatttc
    15121 aataccgctg cgttttcagg taaagtgcct cgcgattggt gtgcttccaa aatatattga
    15181 gcaaacagtg ctccgatttg gtttccggtc aatacttcat aggagccatt tggcaaacga
    15241 acggctgcac ctaaacggtc tgcatcagga tctgttgcaa tcagcaaatc agcgccttct
    15301 ttctcgccaa gtcgtatcgc atactcaaaa gcagagtgtt cttctggatt tggtgatttg
    15361 actgtagtga aatctgggtc tgcgacagct tgttctggaa ctagtacgaa ttgttcgaat
    15421 ccagcttgtt tcaacgcacg ttcgcccaac atttttcctg ttccgtgaag tggtgtgtac
    15481 acaagtttca aatctttccc cattgtttcc accaattcat gatcaatcgt tactgctttg
    15541 atttcttcta gataagcggc atccacttct tctccgatga tcgtgatcaa tccgctatgt
    15601 tcagcttctt cgtcagataa tacttcaact tttaacggat tgctgacttc acggacatat
    15661 ttcgtcaatg catctgcgtc tgcaggaggc atttgtcccc catcttctcc gtagacttta
    15721 tatccgttgt aagctgctgg attgtgggat gcggttacca tgattcccgc aaacgtcttc
    15781 aaatgacgga ctgcaaaaga aagctctggt gttggacgta gactttcgaa tacataagat
    15841 gggatattgt gatgtgctaa tgttttggct gcttccatgg caaattctgg tgattgatgg
    15901 cgtgagtcat aagcgatagc gacacctcgg cgttttgttt cttcgccttg tgtatccatg
    15961 aaacgtgcaa gtccttctgt tgcttgtctt accgtataga tgttcattcg attgatccca
    16021 ggccctaaga ttccccgcat gcctgcagta ccgaattcca acggtgcata aaaagcatct
    16081 tcaagttttt caggatcttc acccagatca tttaattgtt tttttaagtt ttcttctaaa
    16141 ttttcttcat gtacccattg ttcatacact tgttcccaag acatgaaaat aacacctctt
    16201 tctgaaatat ggttcttaat aagtatagca ttcacttatt agatatgaaa gtatgaggac
    16261 caaaaaacgc ttaaatcaag aaaggattct tttatcaagc acggttgcaa gtattcgcat
    16321 aaatcggatt cagtagattt cacgcgaaaa aaaagagtaa aagaacaatt aaaccgttct
    16381 tccactcttt ttaaacttat gcagttgttt cttctaaaga ttccaagtat ttatacactt
    16441 gttgtccagc gatcccacca tcaccaacag cagtagtgat ttgtcgcaaa tctttttcac
    16501 gaacatctcc gatggcaaaa acgcctggta tattcgtctt catttcgttg tctgttggaa
    16561 tccagccagc ttcgttcgtc aaccctgctt ctctaaatgg tgcagttaat ggatccagtc
    16621 caacatagat gaatactccg tctgctgcca aatcaaactc attgccggtt ttgacattac
    16681 gtgctttcac acctgtcact accatttcat tcccaacgat ttcgtcaacg actgtatccc
    16741 acataaagga aattttttca ttcgcaaatg cacggtcttg gatgatcttt tgtgcacgta
    16801 attcatctct acggtggacg atgactactt cagaagcaaa ttgtgtcaaa tagatggctt
    16861 cttctactgc tgagtctcct cctccgatca ctactagacg tttgttacgg aaaaacgcac
    16921 catcacaaac tgcacagtaa gaaacgccac gtccagcaaa ttcttcctcg ccaggtacgc
    16981 ctaatttacg gtggatacag cctgttgcga taatgactgt ttttccttcg taagttttat
    17041 cttcacaaat aacttctttg tggctgccgt gatccttgat atcttgcacg attccataag
    17101 cattttctgt cccaaatctt gaaacacctt catacatctt ataagctaga tcaggaccca
    17161 ttattgaatc gaaaccagga tagttttcga tttctgccgt attgttcatt tggccgccag
    17221 gggcgccttg ttcgatcatc aatacggata agtttgaacg tgaagcatat aaagcagcag
    17281 tcatcccagc aggacctgcg ccaataacga ttacatcata catatgtaga ttctccttta
    17341 cttaatcaag taagcatact ttacagccaa tgcgaaaaaa agtctattgt tctgcttgcg
    17401 tttctcgaat agctttatta tactcgaaaa atccgctgaa aaacaataga cttttataaa
    17461 tcattgatca atcattcgaa agattttctc catttgaatc aattactttt ttataccagt
    17521 tgaatgattt cttcttcgaa cgggctaatg tgccatttcc ttcgttgtca cgatcgacat
    17581 aaatgaatcc gtaccttttc ttcatctcac ctgtaccagc tgatactaaa tcaatacatc
    17641 cccaagtcgt atagcctaat agatcgactc catctaattc tactgcatct ttcattgcct
    17701 ggatatgtgc tgccagatag tcgattcgat agtcatcttc cacataacca ttctcatcgg
    17761 gcgtatcgac tgcacctaag ccgttttcta caataaatag tggtttttga taacgatcat
    17821 aaatatcatt catcgttatt ctcaagccta atggatcaat ttgccaaccc cattcacttg
    17881 cttccagata tggatttttg acagaagcaa agatatttcc tgcagtctgt tccaagagtt
    17941 cgttgtcagt agtcgaaact cgagaagaat aataagaaaa ggagataaaa tcaacagtat
    18001 gttctttcag caatgcggca tccccaggtt ccattttgat ctgaatgcca tttcgttcca
    18061 tttctttcaa tgcataagca ggatattctc ctcgtgactg gacatcaatg aagaaataat
    18121 tttctcgatc tgattttcta gaagcccaaa catcttcagg attgcaagtg tatggataat
    18181 atgctcctgc tgccagcata cagcctactt tgttctccgg atctacttca tgagcaatct
    18241 tcgttgcgat ggcacttgca accaactcat gatgtgccgc ttgatatttg atttgttctt
    18301 cattttctcc ttcttcaaaa tacagccctg cgcccatgaa tggtgcatga aggatcatat
    18361 tgatctcatt gaatgtcaac caatatttga caagtccttt atagcgattg aagatgactc
    18421 ggcacagatt ttcataaaaa ccaacgagtt ctctggaacg ccacgcacca tattctttga
    18481 tcaaatgcat cggacaatca aaatgtgtaa tcgtcacaag cggctcgatc ccgtactttt
    18541 gacattcttt aaagacatcc tcataaaatt tcaaaccaga ttcatttggc tctttttcat
    18601 cgcccattgg aaaaatacga ctccaagcaa tggacatccg ataagtcttg aaccccattt
    18661 ctgcaaataa agcaatatct tccttataat gatgatacat atcgatcgca atttttgctg
    18721 gataaaaatg attttcatca aattcaaaca ttttcttttt tccagtaatg acaggaaaac
    18781 ggtcttctcc gattggcaca acatctacgt tcgctaaccc acgtccgcct tcatcataac
    18841 cgccctcgca ttgattggct gctgtagctc cgccccataa aaaatctttt ctgaatccca
    18901 tcatacttcc tccattacat gtgtattgct tgataattct cttgccagtt catcttggac
    18961 ttcgattcct tctgctttta accggctgat tgtttccttg aattgcggta aatactgctg
    19021 atgtgcaata aacaactcat tcatgatccg ttttgctgtc tttcctgatc gtataagcgg
    19081 attgatcgta aacgcttgta aagctgttcc ataatcacca gtcacagctg cttcgatcgt
    19141 cagcaattcc atattcttca ttacttgcaa ccagcctctt tcagcagcag gaagggaacc
    19201 aaacgctaca tttcgtgcgc cttgtgcgcc aacataggca gtgacttcta ctacacaatc
    19261 tgccggcaga tcaggaacag ctccatcatt ctttgtagat accacgatct gtgtttcttt
    19321 atttccatag atcgaagcaa tcgtttcaca ggcagcatct gaataataag caccacctcg
    19381 tttttgcaat tgttcaggtt tatgatccaa ttctggatct ttgtataatt caaacagctc
    19441 tgcttctgtt tctttcactt gctgcgctct tgtaccaatc gtttgatatt cttctaaagc
    19501 atgttccagc atttcttctt cacggtagta atagcggtgg tagccacaag ggatcatgtt
    19561 catttgtttt aattgttctt tgaaaaacgg gatatcaaag atatttttcg gcaatccatt
    19621 gtcttcatcg tataaacgat caatcaattc tgccgtgcgg tcatttcctt tattatctgc
    19681 tactttgtgc caatggaagt gatttagacc ggcaaacttg tagatcagct cttctttttt
    19741 cttgccaagc attgttggtt ctgtcatcat cgccattact ggcacattgc ataatcctat
    19801 cactttatcc catttgccat atctgataac tgcttctgtc accatcccgc ttgggttcgc
    19861 gaaattgatc agccaagcat ttggacataa acgcttcatg tcttcaacga ttgccaaaac
    19921 cactggaacg gtacgaaaag ctttgaatat cccgccagca ccatttgttt cttgcccaat
    19981 catcccataa taactaggga tccgctcgtc tttgattcgt gcatctaatt gaccgacacg
    20041 aaactgagtc gtaacaaaat ccgcatcttt caatgcttct tcccgatcca gagttgcata
    20101 gattttgact tcatacggag aagcatccca catacgctga gacatctgac tgacgatctc
    20161 aagcttttct tttccttctt ctacatctac gagccagatt tctcgtatcg gtaattcctc
    20221 atatcttttg ataaatcctt ccatcaactc gggggtgtaa ctgctgcctc caccaatcgt
    20281 agcgattttg attccttttg ccatgttctc actcctttac tttttacact tttattatct
    20341 tttttgtaaa caaaacaaac aatagaaatt cgtttctttc cagtaacatt tacaaaagtt
    20401 agtgttttct actggatttt tgtaaatatc ttgtcaaaaa atgtctttat taagctatat
    20461 aacataaatc cgtttacaaa tcattttcaa aaaagttata atttacgtaa aaaaacaaga
    20521 agaaggtatc tacatgttat tacgtgaaaa aatcaaagaa atctcctttt ctccttcaga
    20581 aaatgaagtg attcagtttc tcttaagcga ttctccagca cttggcgaaa tgaccatcca
    20641 agagattgct gagcgatgtt atgtccaccc gtccacactc atccgtgtag caaaaaaaat
    20701 gggttttgat ggctgggtca gttttcgtaa tgctttttta gctgaaacag cctatcttga
    20761 agggaatttt caagacgtag atgcgaatct tcctttttcc accacagacg gtattatgac
    20821 gatcgcaaat aaaatggctt ctttagaaca gatgacgatt caagatacac tttctctcat
    20881 tcatcatgac caactgcaaa aagccaaaca actaatgctt caagcaaaaa aaatcgtgat
    20941 tttcgccagt aactcgaata ctttgatttc tcaagatttc atgctgaaaa tgaagcgcat
    21001 caaaatggat gtcacgattg tcaccacttt tggtgaatcg ttctatgaag cgtacaattg
    21061 cacgagtgat atgtgcggga ttctgatttc ttatacgggt gaaaacaatg tgatccttcg
    21121 taccgcagaa atattgaaac aaaatcatgt tccaatttta gctttgacca gtatcggtga
    21181 ttcaacgcta tcttcaatga gtacagccgc tttgcgactt acgacacgcg aacgactgta
    21241 ttcgaaaatt ggtaatttta cgatcaatac atcgatttgt tatcttctag acgtgttata
    21301 tagttgtgtt tttgcagaaa attatcaaca gaatttagtc catctaattt ctgtaggaca
    21361 aaaatttgat aaacgcccta tcagctcagc catcatgaaa gagcctttca attttgaaaa
    21421 taaagaagtg gactaaaaag aataattaag cctagtccat tctttcacct aaccgttcag
    21481 gtcagtgcgg tgataagtgc atcgtctggc ccgaccgtct ccccttgatt ttccaaaata
    21541 tccaaataat ccgtgtaatt ggtgacgatc acaggtgttt ctaaactgta tccttcctct
    21601 tcgattttct ttttgtcgaa gcggagcaac agatcgcctt gtttcactct ttctccttgc
    21661 tgcaccaagc tttcaaaata tttcccttcc aactgaacag tatccaatcc gacatgaatc
    21721 agcaattcca gacctgcttc agaaaccatt ccgattgcat gtttcgtcgg gaacagggtc
    21781 atgactgttc cgtcaaaagg ggcacgtact tctccttttt cagggtagat caagattcct
    21841 cgacctagtg ttccttgtgc aaaagctgca tcctttgctg cattcagtgg agcgatttgt
    21901 ccttgtatcg gtgtttttac aatttcttta cctgatcttt tctgtctatc atctgtttca
    21961 cctgcttcct ccttccaaaa gaaaaaagtc agcgcaaagg ataccaccat cgcaatggcg
    22021 attccaatca atacattaat cacatccgtt aaatcaccat ctggtctaat atagttggga
    22081 attgtaaaga tgcctaatcc acctggattc caagaagtta aattcattaa tccactatac
    22141 gcgcccccta acgcaccacc gacacaggta aaaataaata tttttttacg tggcaatgta
    22201 aacccataaa tagctggctc tgtaatcccc acaataccag aaacaatggc tggtattgcg
    22261 attgctttgt cttttgggtt tctaagctta aagtagagtg cagtaagtgc tgcagtttgt
    22321 ccaaaagaca ctactgcaac aggtgtcatg atattttgcc aaccattcac agaaaattgc
    22381 atcaatccca ttggtacgat tgcccaatgg agaccaaaca tcactagaat ttgccagaag
    22441 aagcctacga ttgctccata aataactggc gaaagtgcta aaagtgtttc aaatccagcc
    22501 attaaaatat cagttgcgaa tgtgaatgct ggtccaacga gtaaaaaacc taagggcatt
    22561 gtaataagaa cggtgaagaa tggaacgaag aaattcgcga tcatctcagg tactaacttt
    22621 ttaattggtt tctgaatttg gctagctaac aacacaataa agataatggg aatgacagaa
    22681 gaagcgtagt tacgcgcaat ccagggaatg ccgaatactg tttgataaat aggtgcttca
    22741 aaaatagtgc cagaaaatat ggtagtcagt ggtgcttctg ctgcaccact caaagcttcc
    22801 agcgacaagc taggattcat taatgctgcc ccaagcaaca tccctacgaa ttcatttact
    22861 ttaaatttac gggcagctgt taaaccgata ataacaggca taaattggaa tacagaatca
    22921 ccgatattat caaacataac aaatgtgcca gaaccgcggt caaatgcgcc taagaagaca
    22981 agtaatgctg tcagtccacg aatcatccca ccagcagcta gaatagcaag gaatggctgg
    23041 aaacagcccg atattccatc aataagcata tcaaacggtc tcattttttc tgttgaaaca
    23101 gcctcatcaa aagtaaggtt tcctgctttt ataacctctt caaacacgac tggcacatga
    23161 ttcccgatga caacttggta ctgtcctccc gctcgcatta ccgtcacaac accgtccata
    23221 tttttcaaga tctcatcatt tgctttttct tcgtttttta atttgaaacg aagccttgtg
    23281 atacaatgtg tcaaaccatt gatattttcc ttcccgccga cattttctac aattttgctc
    23341 gctaattctt gatattttcc catttctatt ccctccaatt tattagtgga aggtttagcc
    23401 aactgaatgt cactaccctt atttcatgcg ttcttgttaa tttatcctcc tttttttatt
    23461 tatttacaac gattctcatg cactaaacgc gcaatatgga tcgttaaata cattttttcg
    23521 tccgtggaca tctggtagtt gaatatctcg gctaaaaagt ctgctatttt ctcaacgcaa
    23581 ccaaacgtat ccttatattt tttttgaata acctggtaca attcctcaga aacttctccc
    23641 acttctgatt tttctccatc taggacgcgt gtcagaaaaa aacgtaggtg tgtgacgaat
    23701 cgaaaaaaat ggactgaatc ttcatcaaat gtaatcttat agtaatagcg gacgatattt
    23761 agaatatcct gtattgttgt agtaaacagg taggtattgt cttgttggct ttgaatgcct
    23821 tgtgcattga ctaaatgcaa tgcgataaat ccggcttcat cttctgataa cttgatgtgg
    23881 tatttatccg agattttctt cactgttttt ttcccgatgg catactcttt aggaaatagt
    23941 tgtttgatat catacagcaa tacatttggt acaaatattc ccattttgtg acgttcgata
    24001 gccgtataca aatgatccgt caatgcaata tacaccgaat cattcaactg gacttgtaga
    24061 gcctcttttg tatattcaat gatttcacaa gaaatttcta agtactcaag aggaatcgct
    24121 tgtgccaatt cttggaactg ttgtgaaagt ccgtcatttg tcaactgaaa ttttttgtcg
    24181 atgcatgagt caaaaatcgg gtcacccact ttctttttga atgccaaacc ttttcccatc
    24241 acgatcattt cctgatttct ttcatccctg gttattacga cgttgttatt cagtactttc
    24301 tcaatcagca tgttttactc ctttccatga tatatagggc tgtatccaaa acaaaaagaa
    24361 aaacccaaaa agccgtccat ggaaccatgg gagactcatc aggtttagct tgcatcaggc
    24421 aatcactatc caagttgtat cgttttcttt atattatcga aagcaagcac ttttgtcaac
    24481 gctttcttag cggctattgg gcacttctga ctagctgtcg tgattttttt taaatctggt
    24541 aattacgaca ctaacgctca cagatgaaga atctgggaag ctctgtttta ttttcactct
    24601 agcgcttagg tcaaaatgct actatgctac tgtcccaact tctaatatac ggtgttccag
    24661 taccagctta tcggtgataa gccaaattta tgcaaaaatc tggaaagcct tttttacaat
    24721 ttttttctta tcacctaaag ctgaacggag ccctgtcaca accacttttt acacaccact
    24781 gctttgcagt ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt
    24841 cccacgttct actatatgac ctttgtctaa aactaaaatc aattctgcat cccgaatcgt
    24901 ggacagccga tgggcaatcg caatcgttgt ccgtccttgt cgcatttttg tcaatccttc
    24961 ttggatcaat ccttctgttt cagtatcgat atttgctgtc gcttcatcca atactagtat
    25021 tttaggatct gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cgctagaata
    25081 gctggcaccg tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaactt
    25141 gtttgcttga acaaactcag ctgcatggcg aatctgttcg tctgtgatag ttggattcat
    25201 catccggata tttcctgcga tatctccata aaacaagaag gcatcttgca agaccaatcc
    25261 cattttctta cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaagat
    25321 ctggccttca taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct
    25381 tccggtgtgc cccactaatg caacggtctc tcccggatta gcgacaaaag agatattgtt
    25441 caatacattg tgctctccat cataagaaaa gcttacattg cgaaactcga tcttacctga
    25501 agtaatcgct tgatctgctc caggattttg ttgcggtgca tattcttctg tatccatgat
    25561 tttcaaaata cgactaccgg caaccattcc atctgtaaag gtgctcaaaa agtccatcat
    25621 ttgagacatt ggattaaaaa atccctggac ataggtcgta aatgcataga tcagtccaac
    25681 ttctacggga gaatgcagcg catcgatccc aaacaaagtc aacgccaacg caatcgccaa
    25741 tgcatataga aaattgatga tcggacttaa cagaatcgaa tttgttttga tcatcgcata
    25801 gcgcgtgtct aaatactcgt tgttcacttg ttcaaattct ttagctaaac gagcttcttg
    25861 gcgaaactgc tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg
    25921 acttaatttt tcccgcatat tccggtaaat acgagaactg aatttctggt aataccagat
    25981 gacgattcct aaaatcggca agaacaccaa agtccacaag gcaatttctc cattgatttg
    26041 aaacatggca atgaaggaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac
    26101 ataccaaaat tcaaatagcg tttctgtgtc atttgtcact cgagaaacag tcgatcctgc
    26161 cggtgtctga tcaaaataac gcatacctag tgtatgcagc ttttcaaata atcttacccg
    26221 aacatactga taggtcttca atgaagccat ggaatacaaa taccattgga aaaaccaaat
    26281 gatacttttg actatgactc caaacaagta cataccagca aaaaacaaaa taacttgtgt
    26341 cgtagccgac tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag
    26401 gacattgatc acagacaggg caaacgcaaa taagatagca acgatgaacg ttcttctaaa
    26461 aggtttggcg aacttcatca gtcgtttgat gatcttcttc tgttctttaa gtggaatcga
    26521 ttttgtccat tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct
    26581 tctaatttct gtttctccca cattcgttca taccagccgc ctaatgcaag cagctcatga
    26641 tgggttcctc gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc
    26701 acgctgctta aacggtgagc tgcaatgatg gttgttttct cttttcttat ttcttttaaa
    26761 ttatgcaaga tagcttcttc tgttttggca tctactgcag aaagtgcatc atctaagatc
    26821 agcaattcag gatttttgat caatgctcga gcaatcgaaa tcctttgttt ctgtccgcca
    26881 gataaagaga ctccacgttc tccaactaag gtatcataac cttttggtaa ttccttgatt
    26941 tcattgttga taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga
    27001 ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca agaagtgatc ttgaggcaca
    27061 tagccgattg atcctaataa agcattcaat gaatattctt tgatcgaaca tccgccaaat
    27121 tgaatttttc cttgataatc atcgtattca cgcatcagta atttgatgat tgtcgttttt
    27181 cccgcaccag ttttcccaac aatacctaat gtttctcctt cttggatcgc aaatttgatt
    27241 tgttctaatg ctggtgattc ttctttcgga tagttaaaag aggaaatagc tacattcagc
    27301 tcgccttttg ccggagtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc
    27361 aaaagttcat tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga
    27421 ccaatcgcaa acatcggcca aacaagcata ccgatatagc taataaatga gaccatctgc
    27481 ccgattgaga tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa
    27541 gacagaccga taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt
    27601 ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct
    27661 tgtccaaatg ttttaatgac tttgatccca gtaatacttt cttgtgtctt atcattgata
    27721 ctagaaaaag cttcttgcga atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt
    27781 gaagtaacgg ctagtaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata
    27841 aacaaaatca tcgcaatgat cgttgttccg cccgtgatca ctgaatctgc aaaggtcaag
    27901 ataccagctc ctgctacatt ttggattgca ttcaaatcat tcgttgcatg agccatcaaa
    27961 tctccagtcc gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc
    28021 tgctgccgaa gttctttctc caaccgagct gcacttcccc aaatattcgt ccgccagata
    28081 taacgaaaaa gatattgtaa gagagctgca aggatcaata ataagatcca ccaggaaatg
    28141 atctttattg ataaattttt atcggcgatc tcatcaatga tgatcccaat cacttttgga
    28201 ggagctaact gtaccaaggc cactgcgaat agggagaaaa tcccaattaa ataatgtttc
    28261 ttttcctgtc gaaaaaacca acctaatttt ctaaaaatcg acattttact cctcctttgt
    28321 ctttatcaag agcataggat tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc
    28381 taacaaatta cgaaataaaa aacggactga aaaaatcagc ccgcaaaaag tctttgggtc
    28441 agaacctatt ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca
    28501 gatggttttt gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga
    28561 tttttttcta aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg
    28621 attagcaaag caataatagc aataagtacg acaatacctg ttgataccat ataaatctct
    28681 cctttctatt tccgaattgc tctcaatgat ttattttact aaagtttatt gaaaaaagaa
    28741 aggcgtattc tttagtttca tgcaaaaaat agtggttttt ttagagaaga acaaaatttc
    28801 tttcattaac cagcaagaca cgccacgaaa aaaaattagg cataccaaat ctttttcttt
    28861 aattttttct ttctgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc
    28921 ttgaataatc aatccaacaa acaatttcat aagcatcgac ttattgaaca tacaaatggt
    28981 ttatctttag ctgaagtaaa tggaacagtg gatgtaccca agggaaaaag tttttttaaa
    29041 acacttcttg cttactctgg accaggtgca ttagtagcag ttggttatat ggatccaggt
    29101 aactggtcca cttcgatcac tggtggacaa aatttccaat atttattgat gtcagttatt
    29161 ttgatgtcta gtctgattgc tatgctttta caatatatgg cagcaaaatt aggaatcgtg
    29221 acccaaatgg atttggccca agcaatccga gcacggacaa gtaagacatt gggtgtcatt
    29281 ttatggatct tgactgaatt agccattatg gcaacagaca tcgcagaagt cattggtgca
    29341 gcaatcgccc tctacctgct ttttgatatt ccattggtaa ttgccgtatt tatcactgtg
    29401 ttagatgttt tcgtcctttt attgctgaca aaagtcgggt ttcgtaaaat cgaagctttg
    29461 gttgtctgct tgattttcgt cattttattc gttttcgtct atcaagttgg cttgtctaac
    29521 cctgattggc acagtttgtt tgccggattt atccctaaca gtcacacatt ctcagactca
    29581 ccggtcatcg gcggcgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc
    29641 atgccgcata acttatatct tcattcagca gtttctcaaa ctcggaagat cgatcaccaa
    29701 gacgaagaag atgtagcaca agcggtacgc ttttctactt gggattctaa tatccagttg
    29761 tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg gtgtggctgt attcaaaacc
    29821 ggttctgtcc aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct
    29881 atgagcaatg gtatcttgat tgctgtagca aaatctggta tcttgtctac tttatttgct
    29941 gtagcacttc ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc
    30001 atcatggaag gcttcatcca tatgcgtatc ccaatttggc taagaagact agtcacacga
    30061 ttgctttctg tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc
    30121 gaagaacata ccgctttgaa taacttgatg aatgaatccc aagtttttct ggcttttgca
    30181 ttgccattct cgatgattcc tttactgatg atgaccaata gccaagcgga gatggggagc
    30241 cgattcaaaa attcatttgt tgtaaaacta cttggctggt tctctgtgat tagtctgact
    30301 tacttgaatt tacgaggatt accaggtcaa atcgaaagtt tcttcggcga gcaagccacc
    30361 tcagctcaac tagcattggc tgatcaaatc gcttatgtaa tcattgttct tgtccttctc
    30421 ctgttggcat ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcgtttg
    30481 caggaaacga tagaagaaat cgagtagaaa ctgattcttt tacacaaagg aggttgtgtc
    30541 agaagtgggc agcttcaaga aataagaagg aatttaagaa aattgctttt caaatttttg
    30601 tgaaatttcg gcttatttcc gaagaagtta cttctggaac accgtgtatc agaggttgtg
    30661 aaaaagctgc cttgcatcaa gtaataagaa cagccaagta aaaacagctt tttatgtttt
    30721 taacttggtt ggacttatta ccgcagatgc aagcttttga gcaccgttta ttcggaaaaa
    30781 ggaactgcga caagactttt gtcacagttc cctttcctac gattagtcaa gtaaaaggtg
    30841 taagaaaaat agctcataaa cgaaagcgac aaaatcaa
//
LOCUS       DACDOA010000026        29245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDOA010000026 DACDOA010000000
VERSION     DACDOA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.026"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..978
                     /locus_tag="IWI39_001041"
     CDS             13..978
                     /locus_tag="IWI39_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9208159.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1035..1265)
                     /locus_tag="IWI39_001042"
     CDS             complement(1035..1265)
                     /locus_tag="IWI39_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9208160.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1351..2238)
                     /locus_tag="IWI39_001043"
     CDS             complement(1351..2238)
                     /locus_tag="IWI39_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9208161.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2327..3550)
                     /locus_tag="IWI39_001044"
     CDS             complement(2327..3550)
                     /locus_tag="IWI39_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9208162.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3567..4646)
                     /locus_tag="IWI39_001045"
     CDS             complement(3567..4646)
                     /locus_tag="IWI39_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9208163.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4777..6504)
                     /gene="ptsP"
                     /locus_tag="IWI39_001046"
     CDS             complement(4777..6504)
                     /gene="ptsP"
                     /locus_tag="IWI39_001046"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9208164.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6504..6770)
                     /locus_tag="IWI39_001047"
     CDS             complement(6504..6770)
                     /locus_tag="IWI39_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9208165.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7249..9483
                     /locus_tag="IWI39_001048"
     CDS             7249..9483
                     /locus_tag="IWI39_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9208166.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9631..9951
                     /locus_tag="IWI39_001049"
     CDS             9631..9951
                     /locus_tag="IWI39_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9208167.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10156..10380)
                     /locus_tag="IWI39_001050"
     CDS             complement(10156..10380)
                     /locus_tag="IWI39_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9208168.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10578..12158)
                     /locus_tag="IWI39_001051"
     CDS             complement(10578..12158)
                     /locus_tag="IWI39_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9208169.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12559..13935)
                     /locus_tag="IWI39_001052"
     CDS             complement(12559..13935)
                     /locus_tag="IWI39_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9208170.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14063..15181)
                     /locus_tag="IWI39_001053"
     CDS             complement(14063..15181)
                     /locus_tag="IWI39_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9208171.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15323..15757
                     /locus_tag="IWI39_001054"
     CDS             15323..15757
                     /locus_tag="IWI39_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9208172.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15835..16368)
                     /locus_tag="IWI39_001055"
     CDS             complement(15835..16368)
                     /locus_tag="IWI39_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9208173.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16462..17640)
                     /gene="mutY"
                     /locus_tag="IWI39_001056"
     CDS             complement(16462..17640)
                     /gene="mutY"
                     /locus_tag="IWI39_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9208174.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17633..18430)
                     /gene="recX"
                     /locus_tag="IWI39_001057"
     CDS             complement(17633..18430)
                     /gene="recX"
                     /locus_tag="IWI39_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9208175.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18537..19919
                     /gene="rlmD"
                     /locus_tag="IWI39_001058"
     CDS             18537..19919
                     /gene="rlmD"
                     /locus_tag="IWI39_001058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9208176.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20093..20671)
                     /locus_tag="IWI39_001059"
     CDS             complement(20093..20671)
                     /locus_tag="IWI39_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9208177.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            20993..21463
                     /locus_tag="IWI39_001060"
     CDS             20993..21463
                     /locus_tag="IWI39_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9208178.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21571..22797)
                     /locus_tag="IWI39_001061"
     CDS             complement(21571..22797)
                     /locus_tag="IWI39_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9208179.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23551..23751)
                     /locus_tag="IWI39_001062"
     CDS             complement(23551..23751)
                     /locus_tag="IWI39_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9208180.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23885..24493)
                     /locus_tag="IWI39_001063"
     CDS             complement(23885..24493)
                     /locus_tag="IWI39_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9208181.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24578..25048)
                     /locus_tag="IWI39_001064"
     CDS             complement(24578..25048)
                     /locus_tag="IWI39_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9208182.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25096..26010)
                     /locus_tag="IWI39_001065"
     CDS             complement(25096..26010)
                     /locus_tag="IWI39_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9208183.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26156..26959
                     /locus_tag="IWI39_001066"
     CDS             26156..26959
                     /locus_tag="IWI39_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9208184.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27137..28084
                     /locus_tag="IWI39_001067"
     CDS             27137..28084
                     /locus_tag="IWI39_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9208185.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28152..28445)
                     /locus_tag="IWI39_001068"
     CDS             complement(28152..28445)
                     /locus_tag="IWI39_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9208186.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28473..29138)
                     /locus_tag="IWI39_001069"
     CDS             complement(28473..29138)
                     /locus_tag="IWI39_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9208187.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
BASE COUNT         8795 a         5904 c         4909 g         9637 t
ORIGIN      
        1 aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat
       61 cttatgaaag accgagatgg taaggtcatc tatgttggta aagcaaaacg gctcaaaaat
      121 agagtaagca gctattttca tcagaataaa cagcattcaa aaaaagtatt acgtatgatt
      181 catcatataa cagattttga tttcgtcgta gtagacacgg aattagatgc tttattgctc
      241 gagtgtcaat tgatccaaca ttaccgtcct ttttataacc gacaaatgaa ttatttcagt
      301 aattataatt atgtacatat taccaataag ggatttgtat taacagatac tcctacggct
      361 cgaacatacg ggccttttcg tctctataaa aaaatgccgt ctattttgcg tataatggaa
      421 gaaacttatc aaatgccttg gttgtctgaa attagtttgt tagctttgcg agtccagctt
      481 cctgacctac aggaaatgac ttttgaacag aaaaaaaagg aactccaagg gctgtttcaa
      541 ggccgaaata aaaagctact gacttacttg aaaaaaagac aacaacattt catctatcag
      601 ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg aattagttac ttattttatt
      661 agacggatcc aggaacaaaa gcagttttta agaacaccat cgctcacctt cagcatgcca
      721 ttagcagcag atgagagtca gaaaaagcac tacctcgttt gttatggtca acttgcagaa
      781 acaatgatta cctctggtga cgtttctcct gacttctatt atgaaaagaa agaagctcat
      841 ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc ctgtacaaat cttgatcagt
      901 taccgcaaaa aattagaaaa agaacagttt gaaattgaac tgttaaacaa aaaagaagct
      961 gaaaagcaac taaattagtt gcttctcggc ttcttttagg catcttcaac ttcaaatgtc
     1021 aattatccta aaatttattg gattaagtca tagatttcca tcgctacgat atcgatatta
     1081 tcaaattcgt attgctgatg agtatctgtt tcagttaatt caaatgtatc ggtcgcttta
     1141 tcataagtta ctgtacactt ctctacgcct tctttttcaa aacggcgaac ttgcagttca
     1201 ttatctgctg actcgaccat agcctccagt cgttttatga ttgcgactaa ttgtgaatgt
     1261 gtcatcggag gtcctccttt tcttatttct gttatattgt atcaaaagca tagcctactt
     1321 gcattgattt tctctaaaaa aactcgtttt ttatgatttt ttttctggta tttttccttc
     1381 aggccaccaa agtttgatca aagcttgggt tccgtcattc tcaaaacctt tatctgccaa
     1441 ctcttcatat agtttcaatg cttgggtagt tgctggcaat acaagatcca ttttttctgc
     1501 ttcttctaat gcaatcttta aatctttgat aaaatgtttg acaaaaaaac caggtgtgta
     1561 atcttctttt aaaatacgag gagaataatt gcttaaagac cagttagctg cacttccacc
     1621 tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt ttttgtgcat agacaagcat
     1681 ttccgtcatt cctgtcatcg tacctgcaat catgatctga ttagccattt ttgtatgctg
     1741 acctttccca gcagaaccat gcaatgtaaa tgtcgtacca aattctttga ataaaggcag
     1801 tacttgatca taaacggctt cttctccacc gaccatgatc gtcagtgtac cattctttgc
     1861 tcctaagtca ccaccggata ccggtgcatc taacgcatct gcgccttttt cttttgctgt
     1921 ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc aaatcaacta aaatatgtcc
     1981 tgagatatct gcagaaaaaa tccccgactg tccatagtag atttcttcta catctttggg
     2041 gtaacctacc attgtaaaga gaatattact ttgttctgct accgcttttg gtgtatctgc
     2101 ccagatagct ccttctgtta ctaaaggatc agcttttgct tttgtccgat tatagacagc
     2161 tacttcgtgt cctgcttcta gtagatggcg tgcgacagca cttcccatga cgcctgttcc
     2221 gataaaacca agtttcattt tttcgacctc ctaaaaatca aaaatccttg cttctatgct
     2281 agtatacgct agaaacaagg attttttcat aaaaaatgtt tgaattttat cttttaaatg
     2341 aatttaattt tattttgatt cgttcaatcg agtcttcttc ttcttttctt cgcatctctt
     2401 cttcatgata aacaaccatg tcttgataaa attcgatcat agcttcgccg aaatgtgtag
     2461 atgaaatatc ataaagttta gcttctaaaa cttttggatc tttaggaact tcactggaaa
     2521 tataatcaat aagtgctggt gcaaagtctt cgtcggtttc aaaagttttc cctaatgaag
     2581 gatgatcgaa caatcggttc aaataagcat ttccttccac gacacaaggt gttccggctg
     2641 ccatcgcttc tgtataagta agcccttgtg tttctgaagt agaagcactg acaaaataat
     2701 ctgccgcttt ataataaagt gctacatcat tgttgtccac ttctcctgtg aactgcacaa
     2761 attcttctaa ctgcagttct gagatcaatt cctctaactc ttctacataa ggtccttttc
     2821 ctacgataac taatctggcg ttaggataag cggagatgac ctgaggaaac ccattaatca
     2881 aggcttggat atttttctca taagaaatcc tgcttaaaga aagaatcatc aaatggtttt
     2941 cctgaagacc tagtgactcc cttaatttgc tgatatcctg ctgagtgata tcagaacgtt
     3001 tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag cggtgctatt acaccatagc
     3061 tgcgtaatgt atcaataaca cgttcacttg gacaaactac acctgtcgta tgatttgcaa
     3121 ataatctaga caagtatttt acatgggatt gcctgatcac ttttccttta ccgatataat
     3181 gaaggtagtc ttcatacatc gtatggtaag tatggatcag aggtattttc aacttcttag
     3241 ctaccatctt cccaagtatc cctgctccga attctgtatg ggtatgaata agatctagtt
     3301 ctagttcttt ggcaaccaaa taagcatacc acatgcctct aacaacaact cttcgatcct
     3361 tgaaagaaat aaatggtaca cttggcattc gaatcacatc tttttccaac ttatccgcat
     3421 taggatcggt cgttgtaaaa atatagactt catgcccatg tctttctaat tcttgtttta
     3481 atgttttgat cgatgtagca actccgctga cttgcggaaa gtaggtatcg gtaaaaaagc
     3541 cgattttcat gtttttcgcc tccaaactag tccttgacct gatttacttt ttttaatttg
     3601 ctagagactt tttcattcag tacactttcg tatacatttt taagttcttc accgatttta
     3661 ttgatacttc gctcttttgc tgtttgataa ccggcttgtg tcgtactcgg tagttcttga
     3721 ttggtaatct gttggatcag tcggataaat tcttcgtttg tcttccccat atagcagttc
     3781 ttgctatctt ctaaccagcc attatacact ggaatatctc gaacaagtac gttttgtttg
     3841 cttgctaaag cttctaaaac aacgatccct tctgtctctt cataagaagg gaaaaagaat
     3901 agatctgcac ctgaatacgc accttctatg atctctccac gaatgtaacc aggaaacagg
     3961 acattttcag gatgatcttc ttttacaatt ttccggatgt tccgaggaat cgagtacatg
     4021 ggaacatgac caaaccaaat aaatgtgtat tcgggcatct tcttagcaat ttctacgaaa
     4081 tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa tgattacttt ttgatccgga
     4141 gacagtttga aatattctct gaacttttgt tctttcattg gatcagggtg atatttctct
     4201 aagtcgatcc cattggaaat agcttgaatc ggcacacgga taccatagct ttccaacaac
     4261 tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt tttggtacaa tcctaccaga
     4321 tattttttta cgataggaga aagctggttc gatctgataa aggaatttcg gaaatcttcc
     4381 tctgtagaat gcgcatgata gacaactttt tttccttctc ttctggcttt attgaccata
     4441 ttatgactat tgattccgta tgtattgatg tgcaggatgt cataatcttc tttttcatct
     4501 agtgtataag agataccgac ttccgtcaga gcacgttttt gatgatcaag cgcacgcccg
     4561 attccagatt ttgcaagcat cttttcactt tcaaaatata ataatacctt caccttacgt
     4621 cccccttcac ttatatagta taacatagcc tacaaactgt cactcaaact taagccctat
     4681 ttttgtctat tcttactgac aaaaacgttt tcttaaaaaa gaacaaaaaa actggcgtag
     4741 ctcttttaca agccacacca gcttttgata cgttgtttat tttacatatt catgaacaag
     4801 ttcaacgact tcttccatca tatcgcattc tttcaatgca cgatcagcaa gttcagccat
     4861 tttcgctgtg tccaagcgtt tcattaaact acgcgtttta agaatagatg tcgcactcat
     4921 tgagaactca tctagaccca tacctactag caatggaaca gctgtttgat ctccagccat
     4981 ttctccacac atacctgccc attttccttc agcgtgtgct gcatcaatta catttttgat
     5041 caaacgtaaa attgatgggt tatatggttg gtacaagtaa gaaacgcgtt cgttcatacg
     5101 gtctgctgcc attgtgtatt gaatcaaatc gttcgtacct acagagaaga aatctacttc
     5161 ttttgcaaac ttgtctgcta agactgctgc tgctggaatc tcgatcatga tgccgacttg
     5221 gatcgtatca gatacttcct tgccttcact tatcaatttt tgtttttctt cttcaaagat
     5281 cgcttttgct gcgcggaatt ctttgagtgt agctaccata gggaacatga tacgtaaatt
     5341 accatgaaca gatgcacgaa gcaatgcacg catttgtgta cggaacattt cgtcgccttg
     5401 ttcagataaa ctgatacgca atgcacgata tcctaagaat ggattcattt catgaggtaa
     5461 ttgcagatat ggtagttctt tatctccacc gatgtccatt gtacgaacaa ctacaggttt
     5521 tccttccata ccttctaata cagctttgta agcttcatat tggtcatctt ctgttgggaa
     5581 gtctggagaa tccatataca ggaattcagt acggtacaga ccaactgctt ctccaccatt
     5641 attatggaca ccaactagat cttttggtgt tccgatgttt gcagctaatt caaaatgctt
     5701 gccgtcagct gtgacagttt ctgcattttt taatttttcc cattctgctt tttgtgctgc
     5761 gtaatctgca cctgcttttt caaattcagc tttttgttca tctgtaggat cgatgattac
     5821 atcgccttcg atcccgttga cagccaaaat atcgccttct ttgactttag cagtaatttc
     5881 ttttgtccca acgattgctg gaatttctaa agaacgtgcc atgatagcag aatgtgaagt
     5941 acgtccacca atgtctgtta caaacgcttt tacaaaattg cgatctaatt gtgctgtatc
     6001 acttggagtc aagtcatgtg caacaacgac cacttcttcg ttgatcattg atggatttgg
     6061 taaagtaacg cctaataagt gcgctaaaat acgtttagca acgtcacgaa tatctgcagc
     6121 acgctcttgc atataagcat tatcttccat tgcttcgaac ataccgatat acatatccgt
     6181 tacttctttt agcgctgatt cagcatttac gctgttgtct ttaatatttt gcttgatttg
     6241 acctaccatt tctggatctg aaagaaccat caaatgggca tcaaatactt gcgcttctgc
     6301 ttcacctaag ctttgcgctg ctttttcacg aatttgctgc aattcttcag tagatttagc
     6361 taatgcagca tccaaacgag cttcttcagc cgcagtatct tctactgttg ccttactgaa
     6421 tgttaaatcc ggttgaacta gcaggtaagc ttttgcaacg gctacaccgt cactagcggc
     6481 aatccctttt agcatttcaa ccattattca gataagcctt ctttcttcat tgtttcaacg
     6541 attgctgcca ttgcatcagc ttcgtcagca ccttctgcag taatagtaac atcagaacct
     6601 tgacctacac ctaaagacat aacgcccatg attgatttca agtttactga tttaccttta
     6661 tactctaagt ttacatcaga gttaaatttg cttgctgttt gtactaatag tgtagctgga
     6721 cgagcgtgga tcccagtttc tgctactacg tgaaattctt tcttttccat attaatcggt
     6781 ctcctttaag tgagtgttgt ttttatgtga gggttttctt ttttagtaat tcagagaatc
     6841 ctaacaaatg ccaccctttc atctgtaaag attaacattt tttttcgtta gatacaacag
     6901 ttttcattca atttcagata aaagtattca aattattccg gattcttttt catccttttc
     6961 ttcacatttt ataacaaacg tgtttatttc ctttttcttg tcgctttctc ttctcctgac
     7021 gtttatcatt ctttcctttg accctctttg ttttcatatg aaacgaatta cttgcttccc
     7081 tttttatttt tagtataata agagtcaaag gtcaggaatg gtcaatagag atttcacctt
     7141 ttctacctca taaatctctt aaaaaactag gggttattct tttacctcat gaaaaacgag
     7201 atgtaaaata tggatgtacc catttttgtg aaaggacgtg aatgattcat gctttgtcaa
     7261 aactgcggca agaatgaggc aacgattcac ttgtatgcca ctgtcaatgg tcagcggaca
     7321 caactcgatt actgtcagag ctgttatcaa aaaatcaaaa atcaacaaaa tggaggtctg
     7381 actaatatgg cccaaaatga tccatttgga tttggcagtt tggacgatct tttccgttcc
     7441 atgtctcgcc aaatgcaaca gcaaggaaat tatgagcaaa caccaccaac acaatttggc
     7501 ggaaacaaca acttcaatgg tggtcagcca cctcaaggca atacagaagg tttattggga
     7561 gaatacggaa tcaatatcac agaacaagca cgacaaggtg atatcgatcc tgtgatcgga
     7621 cgcgatgacg aaatcaaacg agtcattgaa attttaaatc gccgaaccaa gaacaatcct
     7681 gtcttgattg gagaaccagg tgttggtaaa acagcagttg ttgaaggttt agcacaaaaa
     7741 atcgtagatg gtgatgtacc gcaaaaatta atggacaaag aagtcattcg tttagatgtc
     7801 gtatctttag tccaaggtac tggtatacga ggacaatttg aagaacgtat gcaaaaattg
     7861 attgaagaga tccgtcaagc agaaaatgtg atcctattca tagatgaagt tcacgaaatc
     7921 gtcggtgctg gttctgctgg tgatggaaat atggatgctg gtaatatttt aaaaccagct
     7981 ttagctcgag gagaattgca aatggttggt gctactacct taaatgagta ccgcatcatc
     8041 gaaaaagacg ctgctttaga acgtcgtatg caacctgtac gggtagatga accaactgtc
     8101 gaagaaacga tcagtatttt gaaaggtttg caaaaacgtt atgaagacta tcatcatgta
     8161 aaatatactg atgaggccat cgaagcagcc gcaactttat ccaatcgtta cgtccaagat
     8221 cgcttcttgc cggataaagc catcgatttg ctagatgaaa caggttctaa gaaaaacttg
     8281 acgatccaaa tcgttgatcc taaaacaatc gagaaaaaac tgcaagaagc agaagaacaa
     8341 aaagttttag cttctcgaga agaagatttc gaaaaagcag cttattatcg cgatcagatc
     8401 aataaacttc aaaaaatgaa agaacgtcag ctcactgaag aggaaacacc tgtgattacc
     8461 gaaaaagata tggaaaaaat cgttgaacaa cggacaggta ttccagtggg tgaattaaaa
     8521 gaaaaagaac agacacaact gaaaaactta gcagatgact tgaaagctca tgtcatcgga
     8581 caagataacg ctgtagatcg agtagcgaaa gcgattcgcc gaaaccgtgt tggattaaac
     8641 aaacagaatc gtccaatcgg ctccttctta tttgttggac caactggtgt aggtaaaaca
     8701 gagcttgcga aacaacttgc ctacgaacta ttcggttcac aagattcaat gattcgtttc
     8761 gatatgtctg aatatatgga aaaacatagt gtctctaaat tgattggttc tcctccaggt
     8821 tatgtcggat acgaagaagc tggtcaatta acagaaaaag tacggagaaa tccttacagt
     8881 cttgtcttgt tagatgaagt agaaaaagct catccagatg ttttgcatat gttcttgcaa
     8941 atcttagatg atggtcgatt gacagatgca caaggaagaa cggtcagctt taaagatacg
     9001 atcatcatta tgacaagtaa tgccggaact ggtaaagtag aagcaaacgt tggttttggt
     9061 gcggcacgcg aaggtgttac tcgttctgta ttgaaccaat taaacaacta ctttacacca
     9121 gaatttctga accgttttga cgggatcatc gaattcagtg cattatcaaa agaaaacttg
     9181 atgacaatcg tcacactaat gctagacgat gtcaaccaaa tgctggcagc acagcaactt
     9241 cacatcgaag ttcctacgaa tgtcaaagaa aaattagttg acttaggcta tgatccatct
     9301 atgggtgctc gtcctcttcg acgcacgatc caagaacaaa tcgaagatgg tatcgctgaa
     9361 ttttatcttg atcacccttc tatccatgag ttgaaagcaa aactggataa agatggaaaa
     9421 atcatcgtta cttctaaacc agaacgtttg gctaaagaat cagccgaaga aacggctgaa
     9481 taaatccagt tttatagcgt gagacggtat ttctttgaga aatgccgtct cattcttttt
     9541 tcagaaaagt caaaatactg ttcattttat aaaaaagaaa ttataataag agaaacaagc
     9601 aattaaaaat acataaaaga ggtgagcgcc atgaaattgg taaatgtaac aaacagccat
     9661 tcccgtcttg tattaaacca gcttgaaaac actgatgccc atctagtcaa ggtctacaca
     9721 gccggaaata ccactattgt ctacactgaa gcccctgaac ataatgaaat tctgctgatc
     9781 aatgacaaac gtaaaatcca gccgaaagaa atcgaagatg ctaaaaatta tttcttagat
     9841 aaaatcaaag acgccgttta ccacgaggat gaaatcaaag taatcgaatt ggcaggcctt
     9901 gttgaaatct cgattccaaa acatgaatta ggcgctacat tagccgtgta aacaaatttt
     9961 gaacataata attaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    10021 acacgtataa acggcgaaag cagaagcaac tccttcggaa ataagctgaa attcacgaaa
    10081 attgcttttc aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc
    10141 agcctctttt tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc
    10201 attttcagtt ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg
    10261 tactttttgt gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa
    10321 tcgtaacatc tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat
    10381 gtgtcacact ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat
    10441 tagacatttc tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct
    10501 ctaaacaaaa tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag
    10561 cttgtgtcaa gacttcttta catcaaactt tttaatttta cattgggata tttatccgca
    10621 aaccagcgtt ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga
    10681 gccaagatat tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga
    10741 gcgattttgt gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga
    10801 tattggaata cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga
    10861 tatgttttat aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag
    10921 gatttttgtt tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa
    10981 gggagttctt cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa
    11041 ttgcctgtat catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc
    11101 gaatctgcca taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac
    11161 cagacatcca ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca
    11221 cgatgggctg gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca
    11281 taaggactta ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca
    11341 acgaaagtct ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact
    11401 ggtgtctgat cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt
    11461 agttctactt cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg
    11521 ggaagcagtc catcttcatc caattctact aatcgttcat cctgataatt ttccggacga
    11581 taaaactcta cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg
    11641 attggccaat tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat
    11701 tccaatggtt cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca
    11761 cgttttttta caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg
    11821 attaccatga ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct
    11881 tcatgtcctg gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca
    11941 gaactagtaa cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa
    12001 ttacctgttt ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt
    12061 aattgttctg taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg
    12121 cgattgtcca cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat
    12181 tcaattattt atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc
    12241 atatagggct gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag
    12301 agacagccta tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg
    12361 gctgctctta ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact
    12421 gaatcagtat agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat
    12481 ttttttgtca gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt
    12541 tcataaccaa gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta
    12601 tcttcgttct tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat
    12661 tttaaggatt cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa
    12721 cgataacttt tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat
    12781 cgtatctaca tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag
    12841 catacgtcct tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc
    12901 atctgattca tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa
    12961 cccaacgacg ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat
    13021 ttcatataac agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat
    13081 tttcttgata tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt
    13141 atgaaggatt ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt
    13201 ttcggataaa acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc
    13261 atccgttcgt gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg
    13321 gagcatttct aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc
    13381 atctctggtc atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa
    13441 acttgtggaa gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat
    13501 cagccctgaa gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta
    13561 caattctcca aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat
    13621 attttttgcc gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc
    13681 tagtgaagca ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa
    13741 attatttgga ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc
    13801 tttttgctcg attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa
    13861 agcattgatc aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc
    13921 agggtcagca ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga
    13981 aaaaaacgtg gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata
    14041 aaaacaagtg tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt
    14101 gcttgtcttc tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa
    14161 aaggaacacc taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga
    14221 taatgatcgt taatggatgg attttcagtg atttcccaat tacattagga taaatgatat
    14281 tcccatccaa ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat
    14341 cattgaagac tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa
    14401 ttaggtttgt caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca
    14461 tatatcctaa aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt
    14521 aattagccaa tgttttattc agttgcccca ataactccac aatttggttt cttctttttt
    14581 ctgggaaaaa ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca
    14641 agatgaatgg tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac
    14701 ctaaactatt ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta
    14761 attgctcaaa atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt
    14821 ttaaccagct ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt
    14881 gctgtactag acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga
    14941 gcagcacaat tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa
    15001 taggattcaa taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa
    15061 aaaatgtccc gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag
    15121 ctatgatcaa taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca
    15181 atagatttcc tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt
    15241 ctttttttat ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat
    15301 acgaaaggaa ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat
    15361 ttacttagat gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt
    15421 gtctcgagaa atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa
    15481 aaaagaaccg caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca
    15541 gcggatggcg attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga
    15601 agcagaaaac tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac
    15661 agctgaaact ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc
    15721 tggcatgcct catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa
    15781 aaacagcaag cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt
    15841 acgcgacaat tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc
    15901 atctccatta ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat
    15961 atcgttcggg taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg
    16021 tttctcgccg tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc
    16081 atcgtccagc aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat
    16141 cattgctatt acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt
    16201 tacccatcgg cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga
    16261 gtgttcgctt gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg
    16321 cttgtaactt tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc
    16381 ttaagcagca ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat
    16441 atcaataaaa atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg
    16501 atctaccagt ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa
    16561 ccacttcgaa gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta
    16621 aaaaaggaga acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg
    16681 tgtctgccaa acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac
    16741 taggtcatca aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata
    16801 ctcctcttgt gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct
    16861 atctctttct tcaaaataat acgctccaga atgattttga agagctgcac tgatataata
    16921 aacgtctttt ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt
    16981 gttagctaaa caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca
    17041 gatagctgaa cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat
    17101 gattttccgc atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc
    17161 aatgcaaaac aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag
    17221 tccaaatgcg atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct
    17281 aatttcttca ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc
    17341 agcttggatg ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt
    17401 ttcttctgga gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa
    17461 ataatcgatc actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg
    17521 ataaggatca cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat
    17581 gaactgatct tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac
    17641 tttcctcttc cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat
    17701 ataagctttg ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc
    17761 atagtttatc tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg
    17821 gaagatcagc tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt
    17881 gttggatttt tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt
    17941 cggctgtctg atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt
    18001 caatgatttc aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt
    18061 tgtcacttaa gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa
    18121 ggtccaattc ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt
    18181 cttttaagta tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg
    18241 ctaggtgaaa accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt
    18301 ctaattcccg tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag
    18361 cttctccttc agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa
    18421 cggttaacat aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg
    18481 taatcaggta ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg
    18541 cagaaacaat tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa
    18601 atggtgaagg aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa
    18661 aagaagaagt actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa
    18721 taaaagtcaa aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat
    18781 gcggtggctg tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc
    18841 tgcttttgca agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc
    18901 cgacgatcgg gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa
    18961 aaaataagca gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag
    19021 tccctataac agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg
    19081 ttcaactatt gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct
    19141 tccgcacaat catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta
    19201 ctcgcagtaa aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc
    19261 ccgaagttgt ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg
    19321 atgaaacgat ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt
    19381 tcgatctttc tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg
    19441 gcgaaggaat caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg
    19501 gagttgggac gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata
    19561 ccgttcctca agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca
    19621 ctcgatatga agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc
    19681 agcctgatgg tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat
    19741 cattattgaa ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg
    19801 ccagagatct ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca
    19861 tgtttcctca gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa
    19921 tgaaagaggt tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat
    19981 agctcttcat gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat
    20041 ttatttaaaa aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga
    20101 tattcacgtc tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta
    20161 aaatcacatt atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg
    20221 gtggaacagt tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag
    20281 gttgattagg attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt
    20341 gtgctgttaa attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc
    20401 tgttagatgt ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc
    20461 catcaatgaa tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga
    20521 cattttgatt taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag
    20581 taatataacc atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc
    20641 caataaacaa taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca
    20701 ttgtaaacgc tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta
    20761 aaaaaggtga aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata
    20821 gaaaacatga gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg
    20881 ttcaactttt taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg
    20941 tcttgctgtg agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat
    21001 gataaaagat attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc
    21061 agggttgctt agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc
    21121 accacctagc tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc
    21181 tctttatttg ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt
    21241 tgtgattcag ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat
    21301 tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca
    21361 gttgtggctg catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact
    21421 attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt
    21481 cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc taatttaggt
    21541 ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc catactcatt
    21601 attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat aactttgagt
    21661 atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat aggtattcgg
    21721 atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt acccttcact
    21781 attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga acatcattga
    21841 agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta attgtttctt
    21901 cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt agatatcatt
    21961 gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg ccggggtgta
    22021 gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat tgttctcaac
    22081 tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt tattgacgat
    22141 tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg gatcttcgtg
    22201 tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac ctactggtac
    22261 atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac cgaccgcatt
    22321 gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag gaatatcgag
    22381 cagttgctca attgaatcca tggccatttc gacaccacca aatgcgtagg catgatttaa
    22441 tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca aactgatgat
    22501 cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag aacgtccttg
    22561 ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa aaggttcttt
    22621 gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt ttttagaaat
    22681 tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg ataatccggc
    22741 aatcagcaaa acgaataccc ctaacaaggt taatagaact ttttgccatc gcttcataat
    22801 acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat cagcaaattc
    22861 actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattat tattttttaa
    22921 aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa gaccgaatac
    22981 acgcagttcg caatcgtttt atcttttttt atgtattttt tccagaaaaa gcgaacaaca
    23041 aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc atctttttaa
    23101 cagtctttgc ctaccgttca gattatcatt cgatttcacg acaatttctt cctatgactg
    23161 gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca tggctcttaa
    23221 aatgactcga actagtgatt gcattaccat catcttttaa tggatagata tgctcctctt
    23281 caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag tatgtatact
    23341 agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct ttaaaatcca
    23401 ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca agtgaacaaa
    23461 tgcatttact tagccaaaaa atctaaaaac ttcataaata gacaaaaaga cacgactctc
    23521 taagaagtcg tgtctttatt caacaaatta ttataattta gtcacttcag cagcttgagg
    23581 tccgcgtgca ccttcaacaa tcgtgaattc tacttcttga ccttcttcta ggctcttgaa
    23641 gccgtctcct tgaattgctg agaaatgaac aaatacgtca tctccgccgt ctactgtaat
    23701 aaacccaaat cctttttcat tgttgaacca tttaaccgtt ccgtgttcca taaatgtata
    23761 tcctccaaaa aagttcttgc aagtaacctt gcaagaacat tatacccaat ttagaaaacg
    23821 ctagcaagct ttcgctcttt tattttccaa attattttta agactgatat gacggaggaa
    23881 atggttattt tgctgcttcg aaacgtttgt ttacttcatc ccaattaata acattccaga
    23941 atgctgcaat ataatctggt cggacatttt tatatttcaa ataataagca tgttcccaaa
    24001 cgtccaaacc tagaattggt gtctttcctt ccatcaatgg agaatcttga tttgcagtag
    24061 aggtaatagc taattttcca ttttccatta caagccaagc ccatccagaa ccaaatcgtc
    24121 cagcggctgc ttttttgaat tcttctttaa aagaagaaaa atcaccaaaa gcttcattaa
    24181 tcgcttcttt tatttctcct gtaggttcgc caccagcatt tggtgccatg atttcccaga
    24241 aaaatgaatg gttagcatgt ccgccaccat tgttacgtac agctgtcttg atatctgttg
    24301 gaatagcgtc catatcagat aataattctt ctattgtttt ttcgcctaat tctgggtatt
    24361 tctcaattgc tgaatttaaa ttcgtcacat aagtattgtg atgcttatca tgatgcagat
    24421 gcatcgtttc ttcgtcaata taaggttcca atgcgtcata tgcataaggt agatctggta
    24481 atgtgtaagt cataaaaaaa ttcctcctca atgttttctt aataatgtct acattacaaa
    24541 gagtaacaaa gagaaggaaa ttcttcaatt gttatgccta gtcgtctttt tcactttgac
    24601 ctacttcttc ttcaaaaacc tcagtttctt taaaaataaa caacttgctg aaaatatagt
    24661 tcgcaaggat aacaataatt tgagtgatga ttttcgccac atactcattg ctgttcataa
    24721 cattgatcaa caataccatt gcccccatgt ccaatccgta agaaaggata cggtagaaga
    24781 agaacttacc gcattcaata agtgcatgtg taaaattcgg tgattttgaa tggaataccc
    24841 aaagtttgtt cgttacaaaa gcaaacaata cagaacaaaa ccacgaaata gaattggata
    24901 aggccatact taaatcaaaa tattggcgtg cgagagcaaa actaacaaaa tttacaatcg
    24961 tagccaaacc accaaaaaag agatagacta gtatctccca atatcctttt gcttctaaat
    25021 atccttttaa ctgtcgatac tgcttcatga gccaacctac cttattaaaa attcttattt
    25081 atcatactct atatttcaat aactttcatc tttatttaaa aaaattaatt ttttttgatc
    25141 ctttgccttt gtctctgctt ggatcgtcac atgtgtaata ccatatttta attttaataa
    25201 agaaattata cgaggatata tcttttcaat ctcgcttagt agctgatctt caaaatcaat
    25261 atgtccgtcg aaaataatta tcttttcgct atactgccaa atatggacat gatgaacatc
    25321 ctttacccca tcgattgctt caatttcttg ttccattttt tcatagtcga tagcaggtgc
    25381 actttgtatc aaaatagcgg cagctttttt gatcaccatt atcgcttcta tcaatatata
    25441 tatcgaaatg atcagtgtaa taactggatc gatccagtag atatcgaaaa aacggatcaa
    25501 aattgctccg ataatcacac ttaccgaaga cagtgtatca cttaacaaat gcaaataact
    25561 agattttata ttcaaacttt catctgcttc ttgtcgtaat aataatactg agaacaaatt
    25621 cgcgagcaat ccaataactg ctactgttag catcaaatga ctatttatct tttctggtga
    25681 agtgaaccgc ttgaatgctt caacggttaa aaacaaggat aatatgatca atatgaatga
    25741 gttgatgaaa gcactcaata tttctaaacg ctggtagcca tacgtacgtt ttttatttgc
    25801 ttcttttcgg gccatttttt gtgccccgta agaaaaaata attgccaatg tatcactaag
    25861 gttatggaaa gcatctgaaa caagagacaa actccctgac ataatccctc caataaattc
    25921 tgataaagtg atcaccgcat tcaaaaccac tactccaaat agtttttttg atgatctctg
    25981 tggctgtgta ggcactattc cttcctccat tttatttata ttggatagtc tttagacaag
    26041 aataacattt ttttgatatt tcaccaatca ttcagctgtt tccttaaagc acttgtattt
    26101 cacttgatac ttcgctagaa ttgcaagtga tgacactact agatgaggag tacttatgac
    26161 aacaaaacaa ttaattatca gttcctttat acgattcaaa gacctaaagg aggcgagaaa
    26221 tgtcccattt tggaaagcca ttgtctatct tttagttttg agtataatta tggctttgcc
    26281 aatctctttt caagtttttc aagtcttaaa caacattcaa ttggatgggc aaaagattgc
    26341 tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag aaaaagaagg
    26401 attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa aacgaacaga
    26461 acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt taaaaaacaa
    26521 acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca ataaccaatt
    26581 agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaagcagc tccgttcttt
    26641 tttaagtgaa gcatctatac cactttggat aaaagtcctg acatttatat tttctattta
    26701 tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg cttttctcta
    26761 tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat tgatctattc
    26821 aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt cttttgacac
    26881 tagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca aaggatgggc
    26941 aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca atagaccctt
    27001 ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat atttctttct
    27061 caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta gagtaaagaa
    27121 aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat ttcgtgatga
    27181 atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag aattacaacc
    27241 agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc aagaaaaact
    27301 attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg atcagctacc
    27361 ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca tccacagtga
    27421 atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc tttttcaagc
    27481 acaaaaggca cagagtgaca tgaaatggtt aggttcatcg gtccattacc aatctatcca
    27541 agggaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg caaaaaaatg
    27601 caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag cagaaggaat
    27661 ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag atttcgtagt
    27721 caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata tgtttgaaca
    27781 ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta agacacaaga
    27841 tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg tttttgagga
    27901 agagcctctt ccacaagatc atcctctttg gaaaatggac aatgtactga ttacttccca
    27961 cattgcaggt ttgacacctg attttcaaaa gaaattaatg gctatttttt taaccaatct
    28021 gaaaagctac tttgccaccc atgagcttca aaccaatcaa gtaaaattgt ctgcaggata
    28081 ttaatagctg aaaacaatat tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac
    28141 cctctctttt tttattgttt tttaaagtga taggttggct cctccaggtt gggatcataa
    28201 tcaacaagta aatcatatcc tttaaagaac cattcatctg cttcctcgat aaaaaaaagt
    28261 gtcccatcaa tttccacttt ctttacagga tgttctggct gttcaactga cattccaatc
    28321 gaaaaaccat catgaacctc tgttttgccg tagacttttc caaaaaaacg tattccgtaa
    28381 gtttctggca gatcaatttc tgagtcaaac cacttttgtg cttttggcgt taccgttaat
    28441 ttcatcccaa caacctactt tctcttttct ttctattgat tcatcttatc gtctagaata
    28501 tccacatcgc ctgtctctcc aataaccagt agattatcat tttcacgaac atattcatct
    28561 gctgcaggag aagcgataac cgtttgatct actcttcgta tggcgactac tgttaagtca
    28621 aaacgttgcc tgaaatttaa ttcagctaat gttttgttga aaaatttagg attggagact
    28681 cggatttctg ctaaagaata tttatctgac aactctaggt aatctaaaat attctttgac
    28741 actaaattat gcgcaatcct tattcccatg tctcgctcgg gatggacgac gcgatcagct
    28801 ccgatctttt caagtactct ggcatgatac tcattttggg cttttgctaa aatatttggt
    28861 acgcccattt cctttaccat cagagtaact aagatactgg cttgaatatc ttctccaata
    28921 gcgacaatta catgatcgaa atttcttatg cctaatgatc gaagtgtcat ttcatcctgt
    28981 gcatttgcca caacagcatg agtggctata ttcatatatt cattgacccg atcttcatta
    29041 ctatcaatag ccagtacttc ttgaccagct tcgatcagtg ttcggcagat acttccgcca
    29101 aatcgtccaa gacctacaat tgcaaaattt tgtttcaccg ttttcctcct ttagtatctt
    29161 cttttctcag tataacaaag cctttacaaa aacaaaagca agcatcatta ttgtaaaaaa
    29221 gccaagaaaa ataatagtta ttttc
//
LOCUS       DACDOA010000027        29099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDOA010000027 DACDOA010000000
VERSION     DACDOA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.027"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1031)
                     /locus_tag="IWI39_001070"
     CDS             complement(<1..1031)
                     /locus_tag="IWI39_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208188.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IWI39_001071"
     CDS             complement(1113..1802)
                     /locus_tag="IWI39_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9208189.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWI39_001072"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IWI39_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9208190.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IWI39_001073"
     CDS             complement(2329..2832)
                     /locus_tag="IWI39_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9208191.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWI39_001074"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IWI39_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9208192.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWI39_001075"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IWI39_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9208193.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWI39_001076"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IWI39_001076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9208194.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWI39_001077"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IWI39_001077"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9208195.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IWI39_001078"
     CDS             complement(6444..7463)
                     /locus_tag="IWI39_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9208196.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IWI39_001079"
     CDS             complement(7636..8523)
                     /locus_tag="IWI39_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9208197.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWI39_001080"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IWI39_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9208198.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWI39_001081"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IWI39_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9208199.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWI39_001082"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IWI39_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9208200.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IWI39_001083"
     CDS             9754..10398
                     /locus_tag="IWI39_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9208201.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IWI39_001084"
     CDS             complement(10454..10957)
                     /locus_tag="IWI39_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9208202.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IWI39_001085"
     CDS             11554..12519
                     /locus_tag="IWI39_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9208203.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IWI39_001086"
     CDS             complement(12618..13790)
                     /locus_tag="IWI39_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208204.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IWI39_001087"
     CDS             complement(13777..14922)
                     /locus_tag="IWI39_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208205.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IWI39_001088"
     CDS             complement(14936..15868)
                     /locus_tag="IWI39_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208206.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IWI39_001089"
     CDS             15985..17064
                     /locus_tag="IWI39_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9208207.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="IWI39_001090"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="IWI39_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="IWI39_001091"
     CDS             complement(17789..18016)
                     /locus_tag="IWI39_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9208208.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="IWI39_001092"
     CDS             complement(18119..20707)
                     /locus_tag="IWI39_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9208209.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="IWI39_001093"
     CDS             complement(20727..21386)
                     /locus_tag="IWI39_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9208210.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="IWI39_001094"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="IWI39_001094"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9208211.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="IWI39_001095"
     CDS             complement(22159..23025)
                     /locus_tag="IWI39_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9208212.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="IWI39_001096"
     CDS             23134..23775
                     /locus_tag="IWI39_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9208213.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="IWI39_001097"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="IWI39_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9208214.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="IWI39_001098"
     CDS             complement(25216..26115)
                     /locus_tag="IWI39_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9208215.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="IWI39_001099"
     CDS             complement(26117..26914)
                     /locus_tag="IWI39_001099"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9208216.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="IWI39_001100"
     CDS             complement(26892..27596)
                     /locus_tag="IWI39_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9208217.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="IWI39_001101"
     CDS             27727..28302
                     /locus_tag="IWI39_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9208218.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="IWI39_001102"
     CDS             28420..29097
                     /locus_tag="IWI39_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9208219.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6168 c         5231 g         9137 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg accacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaa
//
LOCUS       DACDOA010000028        28966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDOA010000028 DACDOA010000000
VERSION     DACDOA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.028"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..323
                     /locus_tag="IWI39_001103"
     CDS             126..323
                     /locus_tag="IWI39_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208220.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            429..911
                     /locus_tag="IWI39_001104"
     CDS             429..911
                     /locus_tag="IWI39_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208221.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(969..1565)
                     /locus_tag="IWI39_001105"
     CDS             complement(969..1565)
                     /locus_tag="IWI39_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9208222.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            1952..3418
                     /locus_tag="IWI39_001106"
     CDS             1952..3418
                     /locus_tag="IWI39_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9208223.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            3597..7124
                     /locus_tag="IWI39_001107"
     CDS             3597..7124
                     /locus_tag="IWI39_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9208224.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            7121..10843
                     /gene="addA"
                     /locus_tag="IWI39_001108"
     CDS             7121..10843
                     /gene="addA"
                     /locus_tag="IWI39_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9208225.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            10912..11253
                     /locus_tag="IWI39_001109"
     CDS             10912..11253
                     /locus_tag="IWI39_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208226.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            11562..12605
                     /gene="pheS"
                     /locus_tag="IWI39_001110"
     CDS             11562..12605
                     /gene="pheS"
                     /locus_tag="IWI39_001110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9208227.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            12610..15030
                     /locus_tag="IWI39_001111"
     CDS             12610..15030
                     /locus_tag="IWI39_001111"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9208228.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(15200..16210)
                     /gene="trpS"
                     /locus_tag="IWI39_001112"
     CDS             complement(15200..16210)
                     /gene="trpS"
                     /locus_tag="IWI39_001112"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9208229.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            16464..17201
                     /locus_tag="IWI39_001113"
     CDS             16464..17201
                     /locus_tag="IWI39_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9208230.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            17215..18030
                     /locus_tag="IWI39_001114"
     CDS             17215..18030
                     /locus_tag="IWI39_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9208231.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKTYQKWFPNETEGKVE"
     gene            18043..18690
                     /locus_tag="IWI39_001115"
     CDS             18043..18690
                     /locus_tag="IWI39_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9208232.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            18706..19362
                     /locus_tag="IWI39_001116"
     CDS             18706..19362
                     /locus_tag="IWI39_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9208233.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            19524..20345
                     /gene="racE"
                     /locus_tag="IWI39_001117"
     CDS             19524..20345
                     /gene="racE"
                     /locus_tag="IWI39_001117"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9208234.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            20348..21703
                     /gene="rph"
                     /locus_tag="IWI39_001118"
     CDS             20348..21703
                     /gene="rph"
                     /locus_tag="IWI39_001118"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9208235.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            21704..22222
                     /locus_tag="IWI39_001119"
     CDS             21704..22222
                     /locus_tag="IWI39_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9208236.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            22329..22829
                     /locus_tag="IWI39_001120"
     CDS             22329..22829
                     /locus_tag="IWI39_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9208237.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(23109..24029)
                     /locus_tag="IWI39_001121"
     CDS             complement(23109..24029)
                     /locus_tag="IWI39_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9208238.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            24194..25207
                     /locus_tag="IWI39_001122"
     CDS             24194..25207
                     /locus_tag="IWI39_001122"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9208239.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(25317..26072)
                     /gene="fabI"
                     /locus_tag="IWI39_001123"
     CDS             complement(25317..26072)
                     /gene="fabI"
                     /locus_tag="IWI39_001123"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9208240.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            26292..26732
                     /gene="fabZ"
                     /locus_tag="IWI39_001124"
     CDS             26292..26732
                     /gene="fabZ"
                     /locus_tag="IWI39_001124"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9208241.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(26847..27608)
                     /locus_tag="IWI39_001125"
     CDS             complement(26847..27608)
                     /locus_tag="IWI39_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9208242.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            27797..28891
                     /locus_tag="IWI39_001126"
     CDS             27797..28891
                     /locus_tag="IWI39_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9208243.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         9471 a         4996 c         6083 g         8416 t
ORIGIN      
        1 aaaacagtac gctataatgc ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag
       61 ttaaatctcc aaagaaatag cagtggtaat gtggagaaaa gttccagcag tttaggagga
      121 ttttgatgac ggaagaaatg attaatttag gagaacaata tgcatgcaaa ccaattggtt
      181 ttacaaaaac ggtcattggt gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag
      241 ttgcccaatg tgctgcagag gaccaagaat tattagatga aaaagcaagc atggttgttg
      301 caaaatatga tacatttgaa taaagaaaac aattggacgt gcggcgtgct gcaagtccaa
      361 tttctttgag aaggtaaatc agtattcttt tcctattttc tggagaagaa aaagcgaggc
      421 gagttgtcat ggtaacattc tttacggttc tattgttttt attcgttgtt gtgatgtttt
      481 tatttctttg gctggttcga aaagaaatta tttatcggac tgtgcgaaat cgatgggtct
      541 acttggtgat acccttctta gtgtcactag tcatttggta tacactcatc agtcatccta
      601 cagcagatga attggcaaaa gggatcttgt cagcaatgat ttttattagt tttttgttgg
      661 acagtagagg aatcacagaa gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc
      721 ctttttctga gatcaataaa attgtattat atcagccaaa aggttctaaa atcgtaaaaa
      781 tgaatttttt ccgaaacgga tggcgaggac cgatgctgaa attttctgca tcattagaag
      841 aattagttcc atttttgtcc caacgcttaa acgatgaggc agagattgat attatgattg
      901 atccagaata aaaacagagg ttgcgacaag atgtttgtca caacctctgt tttttatttc
      961 aaataaaatt atccaaatac gcggaagcga tcggcatcgt tcatgtattc tttttcctca
     1021 gccggtgttt ctggtgaacc gaatactaat tgcgaacgta gtttccagtt tgaagggata
     1081 ttccattctt ttgcaactgc ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc
     1141 aatcctttat caactaatgc tacccaagta ttagctgttg cgattccgtt tgattgttct
     1201 gaccaatctg ggaagttgtc tgcatataat tcaaattgtt cttgtagacc ttttaccacg
     1261 tcagtatctt tgaagaataa tactgtacca taaccatttt tgaaaccagc taatttgttt
     1321 tgtgtatttg ggaaagcttc tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc
     1381 caaagttttt catgtgcatc tccgaaaagg ataactgcgc gtgttgattg agcattgaaa
     1441 gcagttgggc tttctttaat tgcttctttg atcaaagttg ttaattcttc attagaaagt
     1501 gaaacattac gtcctagatg atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt
     1561 gtcataataa aatttctcct ttgatttgcg ttttgcattt ttttaattga ttacgaaatg
     1621 aagtatagca ccttacaaaa agtaagtaaa cagatatgct cacaaaaaat aagcgaagtt
     1681 caaaaaacaa ttttaataag aaaaatcgtt atgtaaggat tttcttagtt atcgctttca
     1741 ttgttgtgtt ttaggaattt ttgcttattt gaaaaaaaca gctgatatat aattattaaa
     1801 tctctatctt ctttttttta tgatattgcc attaaaaatt acttgctttt atagaaaatt
     1861 agtttatatt tcttaaataa gttttttagt taaatttttc ggtaaagatg tataatattt
     1921 tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt
     1981 cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta
     2041 ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact
     2101 tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat
     2161 gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt
     2221 cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag
     2281 tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt
     2341 ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc
     2401 gattttttac atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat
     2461 cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga tgttctttgg
     2521 attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc
     2581 gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt
     2641 tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt
     2701 tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct
     2761 gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt
     2821 ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt
     2881 tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca
     2941 atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa
     3001 acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg
     3061 attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag
     3121 tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc
     3181 agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc
     3241 tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta
     3301 cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg
     3361 tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc
     3421 aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctgttttttg cgtatactta
     3481 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
     3541 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
     3601 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
     3661 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
     3721 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
     3781 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
     3841 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
     3901 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
     3961 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
     4021 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
     4081 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
     4141 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
     4201 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
     4261 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
     4321 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
     4381 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
     4441 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     4501 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     4561 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     4621 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     4681 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     4741 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     4801 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     4861 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     4921 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     4981 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     5041 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     5101 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     5161 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     5221 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     5281 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     5341 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     5401 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     5461 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     5521 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     5581 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     5641 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     5701 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     5761 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     5821 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     5881 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     5941 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     6001 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     6061 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     6121 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     6181 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     6241 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     6301 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     6361 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     6421 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     6481 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     6541 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     6601 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     6661 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     6721 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     6781 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     6841 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     6901 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     6961 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     7021 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     7081 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     7141 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     7201 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     7261 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     7321 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     7381 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     7441 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     7501 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     7561 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     7621 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     7681 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     7741 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     7801 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     7861 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     7921 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     7981 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     8041 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     8101 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     8161 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     8221 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     8281 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     8341 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     8401 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     8461 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     8521 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     8581 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     8641 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     8701 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     8761 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     8821 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     8881 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     8941 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     9001 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     9061 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     9121 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     9181 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     9241 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     9301 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     9361 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     9421 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     9481 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     9541 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     9601 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     9661 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     9721 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     9781 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     9841 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     9901 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     9961 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
    10021 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
    10081 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
    10141 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
    10201 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
    10261 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
    10321 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
    10381 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
    10441 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
    10501 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
    10561 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
    10621 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
    10681 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
    10741 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
    10801 aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat tgagcaaact
    10861 tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat gatggagcta
    10921 acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat tttaggaaaa
    10981 aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg ctttgttgag
    11041 cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt gaaggaatta
    11101 gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg ttcagaatat
    11161 ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca agtttggagc
    11221 gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct tgtaaactat
    11281 agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta aaccagttca
    11341 gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt tcactttccg
    11401 agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt gcggtgtaag
    11461 tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca ggaatgaggt
    11521 ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta caagcacaat
    11581 tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat ttaaatgaat
    11641 taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa gtattacgtg
    11701 ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca aatgagatcc
    11761 gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa gaagcgttaa
    11821 atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa gtaaaacatg
    11881 gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt gtgggtatgg
    11941 gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc gaacggatga
    12001 atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt tcagacgaga
    12061 tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa catgatttct
    12121 ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat acagatgatg
    12181 cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa aacatcacaa
    12241 tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg gaagatcgtg
    12301 aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa gtagatgtca
    12361 gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga tggatcgaaa
    12421 ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc gatccagaag
    12481 aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg cggtatggag
    12541 tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag ttcaaggggg
    12601 agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt atcgaatgta
    12661 acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga gggagtaacg
    12721 gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg cgtaccacat
    12781 ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga actgtctcaa
    12841 atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc acttcctaac
    12901 tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca agtatcaaat
    12961 ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc gaaagaatac
    13021 gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt ttttccttat
    13081 ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc ggatgcattg
    13141 tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc tacattcgat
    13201 gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt ggaagttgaa
    13261 gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag aatccagcca
    13321 agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat caacaatgta
    13381 gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc atttgactac
    13441 gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga aacattagta
    13501 acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac taacggcaaa
    13561 gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac gaatgaaaca
    13621 acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg tacagcaaaa
    13681 caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa ccatgcgact
    13741 gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg tggggaagtt
    13801 ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt gaagatttca
    13861 cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt aaatgagatt
    13921 tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt agtagtacca
    13981 ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc tcgtatttat
    14041 ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg aagcttgtca
    14101 aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg attgagcgaa
    14161 gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat aaatgaaagt
    14221 aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg aatgaatttg
    14281 atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac agagattgct
    14341 ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca tctgccgcaa
    14401 gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga ttggcagacg
    14461 aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt atttgaacaa
    14521 ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga aatgcatcct
    14581 ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg tcaagtgcat
    14641 ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt tgatttatct
    14701 gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa attcccagca
    14761 gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga attaacagaa
    14821 gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga tgtctatcaa
    14881 ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt cgtcaaccca
    14941 gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga aaaagcgttg
    15001 gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta acgatcaagt
    15061 agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca catctctttt
    15121 tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct ttacagcctg
    15181 cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat tttacttcag
    15241 aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag atttccataa
    15301 cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca aattcagctt
    15361 ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg cggcggtaat
    15421 gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag taggtgaaga
    15481 ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga tcagtataca
    15541 tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata ccgtttccta
    15601 acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt ccttttggtg
    15661 ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag ctttgtacga
    15721 tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca agattcgctt
    15781 tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct gtaggcacgc
    15841 tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc cgaacgcgtc
    15901 ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga atttgtgatt
    15961 ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct aaagcaactt
    16021 gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt gcttgcatgt
    16081 cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact cgggttttta
    16141 gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc gttaaaataa
    16201 tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa tatcttaccg
    16261 tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg ataaaataac
    16321 taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag aaactttaca
    16381 aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga aatcttacta
    16441 aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt
    16501 atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg
    16561 ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag
    16621 agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa
    16681 aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa
    16741 ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg
    16801 caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag
    16861 attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt
    16921 tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa
    16981 tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg
    17041 tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg
    17101 atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag
    17161 tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc
    17221 aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga
    17281 tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa
    17341 atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga
    17401 actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac
    17461 aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac
    17521 ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt
    17581 gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa
    17641 atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca
    17701 tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat
    17761 gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg
    17821 attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact
    17881 aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac
    17941 caagcgctag aggaaatgca tgcagatggc acatacgata aaacctatca aaaatggttt
    18001 ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa
    18061 acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca
    18121 cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa
    18181 gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc
    18241 atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa
    18301 gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca
    18361 gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat
    18421 tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta
    18481 ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta
    18541 gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat
    18601 atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat
    18661 cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc
    18721 gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt
    18781 tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt
    18841 gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt
    18901 ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg
    18961 aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa
    19021 attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg
    19081 ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg
    19141 atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc
    19201 atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat
    19261 gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct
    19321 ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg
    19381 gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc
    19441 gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag
    19501 gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta
    19561 ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg
    19621 aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac
    19681 aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc
    19741 tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt
    19801 ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa
    19861 ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag
    19921 cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt
    19981 cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat
    20041 tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat
    20101 tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag
    20161 cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc
    20221 aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa
    20281 tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa
    20341 aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac
    20401 aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat
    20461 ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg
    20521 ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag
    20581 cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt
    20641 gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt
    20701 gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa
    20761 attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca
    20821 tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta
    20881 ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt
    20941 cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat
    21001 gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct
    21061 aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac
    21121 aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt
    21181 aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga
    21241 agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc
    21301 agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg
    21361 ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac
    21421 agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc
    21481 caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc
    21541 tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc
    21601 agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa
    21661 attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt
    21721 aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga
    21781 agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga
    21841 tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt
    21901 gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt
    21961 tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg
    22021 tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag
    22081 catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga
    22141 tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt
    22201 ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat
    22261 tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag
    22321 gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt
    22381 tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac
    22441 ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg
    22501 taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca
    22561 tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg
    22621 atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg
    22681 ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg
    22741 taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat
    22801 taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat
    22861 aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag
    22921 cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat ttttgtgaat
    22981 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
    23041 ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg ttgcatcgtt
    23101 tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc agaagctgtc
    23161 aattctttga cataggagct caagaactct tcttttaagg catattgttt tccgttcaat
    23221 gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa tccaaagata
    23281 gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt tactcgatca
    23341 atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc taaaagtact
    23401 tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg gatgaaatgg
    23461 acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac gacggttcct
    23521 tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg ttccatgata
    23581 ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc gattgccaca
    23641 cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc agataaaatt
    23701 gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag actatgtaaa
    23761 gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa agacttatca
    23821 atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa aatcagatat
    23881 aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc ccaaaaccgc
    23941 tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga agagtctgtc
    24001 gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt tgattctttc
    24061 tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata atgaaggaaa
    24121 aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag aaaataacga
    24181 aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata tcaaaactcc
    24241 ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat tgacagaaga
    24301 taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc aatgttttga
    24361 aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac ctgaacaaaa
    24421 tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc ctgaaaagat
    24481 gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc cgccctatgg
    24541 aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa acattggtgt
    24601 ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag cgtatttcaa
    24661 aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg ctcctaaggg
    24721 gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag gaacagaagc
    24781 aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata cgaaaatcga
    24841 agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca tcactccgca
    24901 atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag ctgaagaacg
    24961 actaggactg caagcaattg aaggagatat tttcattgat gatctagatc aatttgcaga
    25021 agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt atcacaataa
    25081 caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac ggttgtatga
    25141 tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga ttgaagtcgt
    25201 ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca ggaagagact
    25261 ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac aaaagtttat
    25321 gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct ggaaagtggg
    25381 ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg ctgatcaggc
    25441 gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt gacagctaat
    25501 gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga gaactcagct
    25561 gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat gttataattc
    25621 gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt tggtgcaaga
    25681 aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc ttgagctaat
    25741 tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt gttggcgtaa
    25801 gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc attcattgct
    25861 ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc ttcgccgacc
    25921 aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat ttctgcccct
    25981 tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc gacgcccata
    26041 atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt ctgataaaaa
    26101 tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat ttcttaatta
    26161 ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa gattgctaaa
    26221 tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg cctgaaagaa
    26281 ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg attcctaatc
    26341 gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa aagatcgttg
    26401 cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct gacaatccag
    26461 tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata cttatcctaa
    26521 aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag gccaaattcc
    26581 gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc aaaatgaagg
    26641 gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc tgtgagtgcg
    26701 agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag caaaacaaaa
    26761 ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat acgttgtcat
    26821 ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag tatattgatt
    26881 ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat atttgcaata
    26941 gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg gccatctagt
    27001 gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc gtcttttaaa
    27061 agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc gttactgtta
    27121 ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg gttgttgatc
    27181 tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc tttaggatcg
    27241 ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt gtagtaaccg
    27301 tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg aaataatttg
    27361 attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc ggcgacatct
    27421 ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt cgtgctttcc
    27481 gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt gttgtttgca
    27541 gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat aactttattt
    27601 ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat aacaaaatag
    27661 agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga aaaagaaaag
    27721 gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac gaaaagatgc
    27781 aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac ggtcaacatt
    27841 tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac gggtttttac
    27901 cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta aagaagtagt
    27961 tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag cgttatttga
    28021 acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg cctacggggt
    28081 ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca atccttttgg
    28141 tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca tcatgagtta
    28201 ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc gatactctgg
    28261 tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata atttgaatac
    28321 gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc aagatggctt
    28381 gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc atttaatcct
    28441 tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag aagaattgaa
    28501 agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata cggaagaagg
    28561 tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc gaggggatcg
    28621 ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata aagatttccc
    28681 gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg aagggtacgg
    28741 tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg gacgtgtcaa
    28801 tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga aaccatggtc
    28861 aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa ataatccgct
    28921 agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DACDOA010000029        26729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDOA010000029 DACDOA010000000
VERSION     DACDOA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.029"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="IWI39_001127"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="IWI39_001127"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9208244.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="IWI39_001128"
     CDS             1286..2461
                     /locus_tag="IWI39_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9208245.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="IWI39_001129"
     CDS             2465..3106
                     /locus_tag="IWI39_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208246.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="IWI39_001130"
     CDS             3103..4020
                     /locus_tag="IWI39_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9208247.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="IWI39_001131"
     CDS             4017..4688
                     /locus_tag="IWI39_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208248.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="IWI39_001132"
     CDS             complement(5229..5717)
                     /locus_tag="IWI39_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9208249.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="IWI39_001133"
     CDS             complement(5918..6184)
                     /locus_tag="IWI39_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9208250.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="IWI39_001134"
     CDS             complement(6184..6447)
                     /locus_tag="IWI39_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9208251.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6440..6712)
                     /locus_tag="IWI39_001135"
     CDS             complement(6440..6712)
                     /locus_tag="IWI39_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208252.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6923..7108
                     /locus_tag="IWI39_001136"
     CDS             6923..7108
                     /locus_tag="IWI39_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9208253.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="IWI39_001137"
     CDS             complement(7250..9073)
                     /locus_tag="IWI39_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9208254.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="IWI39_001138"
     CDS             complement(9671..12376)
                     /locus_tag="IWI39_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9208255.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="IWI39_001139"
     CDS             complement(12682..13629)
                     /locus_tag="IWI39_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9208256.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="IWI39_001140"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="IWI39_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9208257.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="IWI39_001141"
     CDS             complement(14264..14626)
                     /locus_tag="IWI39_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9208258.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="IWI39_001142"
     CDS             complement(14637..15758)
                     /locus_tag="IWI39_001142"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9208259.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="IWI39_001143"
     CDS             complement(15774..16124)
                     /locus_tag="IWI39_001143"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9208260.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="IWI39_001144"
     CDS             complement(16254..17765)
                     /locus_tag="IWI39_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9208261.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="IWI39_001145"
     CDS             complement(18087..19439)
                     /locus_tag="IWI39_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9208262.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="IWI39_001146"
     CDS             complement(19592..20668)
                     /locus_tag="IWI39_001146"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9208263.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="IWI39_001147"
     CDS             20864..22609
                     /locus_tag="IWI39_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9208264.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="IWI39_001148"
     CDS             complement(22889..23326)
                     /locus_tag="IWI39_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9208265.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="IWI39_001149"
     CDS             complement(23310..24683)
                     /locus_tag="IWI39_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208266.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="IWI39_001150"
     CDS             complement(25005..25493)
                     /locus_tag="IWI39_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9208267.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="IWI39_001151"
     CDS             complement(25515..26117)
                     /locus_tag="IWI39_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9208268.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            26216..26665
                     /locus_tag="IWI39_001152"
                     /pseudo
     CDS             26216..26665
                     /locus_tag="IWI39_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         7718 a         5634 c         4954 g         8423 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
    26161 tatgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg
    26221 gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg
    26281 ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag
    26341 gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa
    26401 atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc
    26461 taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga
    26521 aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa
    26581 gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag
    26641 agcagagaac gcatatttaa aaaagtggaa tggcaacact ttttttgaca aaaattataa
    26701 ggtgttgtga atgctgcggt gttatggct
//
LOCUS       DACDOA010000030        26140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDOA010000030 DACDOA010000000
VERSION     DACDOA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.030"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            115..1155
                     /locus_tag="IWI39_001153"
     CDS             115..1155
                     /locus_tag="IWI39_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9208269.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFSQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1207..3351)
                     /locus_tag="IWI39_001154"
     CDS             complement(1207..3351)
                     /locus_tag="IWI39_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9208270.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(3708..5081)
                     /locus_tag="IWI39_001155"
     CDS             complement(3708..5081)
                     /locus_tag="IWI39_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9208271.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(5081..5476)
                     /gene="rbsD"
                     /locus_tag="IWI39_001156"
     CDS             complement(5081..5476)
                     /gene="rbsD"
                     /locus_tag="IWI39_001156"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9208272.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(5504..6421)
                     /gene="rbsK"
                     /locus_tag="IWI39_001157"
     CDS             complement(5504..6421)
                     /gene="rbsK"
                     /locus_tag="IWI39_001157"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9208273.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(6529..7542)
                     /locus_tag="IWI39_001158"
     CDS             complement(6529..7542)
                     /locus_tag="IWI39_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9208274.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(7623..8222)
                     /locus_tag="IWI39_001159"
     CDS             complement(7623..8222)
                     /locus_tag="IWI39_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9208275.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            8408..9109
                     /locus_tag="IWI39_001160"
     CDS             8408..9109
                     /locus_tag="IWI39_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208276.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            9119..11830
                     /locus_tag="IWI39_001161"
     CDS             9119..11830
                     /locus_tag="IWI39_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9208277.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            12281..13909
                     /gene="treC"
                     /locus_tag="IWI39_001162"
     CDS             12281..13909
                     /gene="treC"
                     /locus_tag="IWI39_001162"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9208278.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(13969..15150)
                     /locus_tag="IWI39_001163"
     CDS             complement(13969..15150)
                     /locus_tag="IWI39_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9208279.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            15402..16925
                     /locus_tag="IWI39_001164"
     CDS             15402..16925
                     /locus_tag="IWI39_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9208280.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(16994..17722)
                     /locus_tag="IWI39_001165"
     CDS             complement(16994..17722)
                     /locus_tag="IWI39_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9208281.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(17735..19015)
                     /locus_tag="IWI39_001166"
     CDS             complement(17735..19015)
                     /locus_tag="IWI39_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9208282.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(19156..19758)
                     /locus_tag="IWI39_001167"
     CDS             complement(19156..19758)
                     /locus_tag="IWI39_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9208283.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            20003..20209
                     /locus_tag="IWI39_001168"
     CDS             20003..20209
                     /locus_tag="IWI39_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208284.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            20521..21240
                     /locus_tag="IWI39_001169"
     CDS             20521..21240
                     /locus_tag="IWI39_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9208285.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(21321..22004)
                     /locus_tag="IWI39_001170"
     CDS             complement(21321..22004)
                     /locus_tag="IWI39_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9208286.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            22223..24409
                     /locus_tag="IWI39_001171"
     CDS             22223..24409
                     /locus_tag="IWI39_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9208287.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            24755..25165
                     /locus_tag="IWI39_001172"
     CDS             24755..25165
                     /locus_tag="IWI39_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9208288.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            25179..25535
                     /locus_tag="IWI39_001173"
     CDS             25179..25535
                     /locus_tag="IWI39_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9208289.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8770 a         4636 c         5129 g         7605 t
ORIGIN      
        1 ttttattaga atatagtaaa ttttttctcg ttactcacgg aataagctgt taagaaaacg
       61 gtttccatgt atactggaat cagtgcaaat gaaagaaaag agggtgcaag gaacatggaa
      121 atcaatgaac aaaattttgg cgaaaactat cacttgatta cgatcacgaa tcaaaatggg
      181 acatctttgt ctgtcagtga tcttggggca cgggttgttc attttcaaac aataatcgat
      241 aatgaaaaac gagagttgat cttaggtttt gatgaagcaa aagaatatct agaaaaagac
      301 ccttatatcg gtgcttcaat cggacgtatg gctggacgga ttacgaatgg cacattcaaa
      361 ttagatgatg aaacgttcga agtaaaaaca gacccggaca ctggtcattg tttacatggt
      421 ggtgcaccta gctttgaagc caagcagtgg cattatacag tcatcaatgg tgaaaacgaa
      481 gcatcggtca ttttctatca gactagccca gatatggaaa acggatttcc tggaacttta
      541 gatgtagaag tacggtatac gctgacgaac gatgatacat ggcgagtgac ggtccaaggg
      601 atcagtgacc gcaaaacagt attcaatcct acaaatcatg tctatttcaa tttaaccggt
      661 gatccagcac agtcaatcga cgaacatata ctttggttga atagccgatt atttgctacg
      721 ctcaataaag atacaacgcc tacaggagaa gtagtttcag taagtggcac agcatttgac
      781 ttccaacgtc ctaaaaaatt aggtgaggtc tttgccagtg attttagcca atcaatgata
      841 gtgggcggca tcgatcatcc atttttgttg aaaaaacaat ctggtttact aaatgctgcg
      901 gaattgacaa gcccagatga aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc
      961 gtgattttta cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa taaattagta
     1021 aatcatggcg gtatcacatt tgagactcaa gaactgccag gagcggagcg acttgaaagt
     1081 tttggagatg tcactctgga acctaaccaa gtacgcgagt ttgtaacgga atacaaaata
     1141 aatataaaga aatagaataa attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa
     1201 tttattctat ttctttaaag gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt
     1261 tagtacagga acagttgcag ctttaactgt acgagtagtt gtttctggta tctgtcttgc
     1321 cgtaaaattg ttccacaaat cccagattga agcgacacgg aagatggaag aagcgttgtt
     1381 gacgcgtttt atcgttgaaa attctaatcc tgtgattggc acgccatctg ttgtccattt
     1441 gattggcaca tcgaatcgct tagacatatt gaggatttca gcgtttgctt cttcagttgt
     1501 tgctgcttct tttttcggtt catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt
     1561 caatttttgt gaatattggc gatctgtagc ataacgtcct tccaaatatt tggttgcttc
     1621 tttgtagtct tttgtttccg acttccaagt tggtttgtag aaatcaggat tgccagtcag
     1681 tccatttttg atcaagtcgg catagtcttc caaactttcc ttgtaagaag gatatttacg
     1741 gaattgtgcg cggatttggt agctttgtcc ggaactatct tgttcaagtg tattgaaggt
     1801 cacgctgctt cctttatatg atcctttgat accaaaaagg ttatgatttg gttcgcttga
     1861 taataagctg tttccagatc cactttcaag gatagcttga gccatcatga ctgatgcgta
     1921 gattccttct ttttgcccga tttcctgtgc atcatctgca atctttttca agaactgatc
     1981 agttgtttgg ttttgactga aatggaacga gccatctaat ccatctaaaa tagatgagcc
     2041 gcctgtcagc gaacctgaag cagcaggtaa acctgctaag tttactcgga cagcaaaatc
     2101 aggcgtgaaa tctgaaatag aagttactcg aactttatct cctggttgag gcgcatggat
     2161 aaattgtccg ttcccaatat agatacctac atggtgagta acaccggaag aaggtcccca
     2221 gaagacgagg tcgccaggtt gggcctgact tactggaatc tgcgtacctg catattgctg
     2281 ttcgcctgtc cagccaccga tataatgacc ggttgctttc atatatacgt aataagtcaa
     2341 accagaacag tcaaaagtat ttggaccttt tgctccccat acatatggtt ttccaagatg
     2401 cttgaatgct tctcgaacga ttgcttgttg gacattcgtt gtatttgtag gcaatacagc
     2461 atcaattggt gattttacaa caaaatcaga agaagcagaa gatggattct ctataccgct
     2521 ttgatttgct gtatttggac gagatgaagc attgttcgtt ccgccattcg atgaaccatt
     2581 gtttggatgg tttggttctc tctgagtatt tccattgtta ttcgatccag atgggtttgt
     2641 ttgattcgaa gaattcccgc tgttcgaatt attgttatta ttgtttgaat tatttgaacc
     2701 attattattg ttgttattac ccacatctgg tttgtttggc gtttccggat ctggtgtact
     2761 agggacttct tcactaggat cagttggttt ctctgttgaa gtatccggtc cagctttaaa
     2821 ttctgcagta atagataaat tgttcgtacc tactgtaaag gtctttgcat cataatttac
     2881 ttctttggca ccattaacag cgactgaagc aatttgttca ccttcagctg gcgtaatagt
     2941 gtaagttacc ttcgtttccg ctaataaacc ttctaccgtt ccactttccg taatatccaa
     3001 aggttgctgt tcaggggaat ccgctgtcga agcagtcagt tggatcttac caatcaaaga
     3061 aggagcaatt gtaagggaat atgtctcggc tggcttttca ttttctgatg gatcagccgg
     3121 atcaatttca gttggctcgg tcgatcctgt tgttgagtta gttgttccag ttgggtcttc
     3181 tgtaaaagaa tccgttgttt cactagtagc tgagctggat gtagtcgtat ctgttgtgac
     3241 tgtttgatca gctatttgat tatctacggc aaatacggta ttggtcgaca aaacagacgg
     3301 agcaatcagc caagtagcag ctagcaaacg tatcgttttt ttatttttca taataaacta
     3361 gtccttttcc tttcattgat tttctgtctt attcagcatt cattagacat tttttggaac
     3421 agataatcgt gtcgaaaagc tttttctttt cataaatctg tctgtgtatt ctaaataaaa
     3481 caatttgtaa ataagcaacg agcattcatt cgtttttctt tgacgttcat tttattgcat
     3541 agaccacctt tgtttgggga taaacaagtg gggataaaaa tggttatgca tatgtaaatt
     3601 ataccaaagt ttggaaagta tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa
     3661 gactgaaata tgtgataaac atctcatact tcagtctttt tcattaacta ttcataagac
     3721 attaggtctt catcaaaccc gctaacgaga gtagtgatca ttgctacgac tagactaaca
     3781 gccagtcctg ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga
     3841 ctaccaaata caaaagcgat cgctttcact tgggcaaatc ccattacagc tccaccagca
     3901 gctgcaccaa tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca
     3961 gttggttcgg tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga gatcgaacgg
     4021 gtttttgaac gtttacttcg cagaaataca cctaaagctg caccagccat tccaaaattg
     4081 gctgctcctg tcaacggaac gattcggtcg aacccataag cagagatatt actgatcatg
     4141 acagggttta cagcccattg gataccaaat attaccaaaa tattccaacc accagcgatg
     4201 attgcgccac tgatcggtcc gttaatatcc atcatccaag taattgcggc agctaaacct
     4261 tcgccaaggt aaacacccgc aggcccgaat accattaacg tcaaaggaac cgtgataact
     4321 aatgaaagta aaggtacaaa tactaattgc atattttcgc gaatatattt tttgaggaat
     4381 ttttcaacaa aagaataaaa gaaaatacct aaaatcgccg gcatgacagt agacgtatac
     4441 tgcatcagta ctacaggaat ccccagaaaa tcagtagctg ctttgccact gtctgaaagt
     4501 aaagaggtca gattcggttc aatcagtgct gcagcaatga cagccgcaat atatccattc
     4561 actttaaacg ttttggcagc agaaaaagca agaaagattg gcagaaaata aaagacggca
     4621 ttcgacgcag cagacaaaat atgataagtt ccgcttgttt cactaagcca gccaatcgta
     4681 gtcgctaaaa caaccagacc ttttaatata ccagaaccgg caaacagcgg aatgatcggc
     4741 gtaaagattt ccgatatcaa accaaatagt ttttcgatcg gatttagctt ttctttcgga
     4801 gctgaatgat cgatttgacg cgtcaattcc ttgtaagcag gagccaatcc ctgatccatg
     4861 attatttctg catatctatc gagtaaaatg aaatacttaa tggaagggat ttgctgtttt
     4921 aatttttctg cattggctac tggctgtttt ttcaatttga tacgaagccg agtcgcacat
     4981 ggaaggacag aatcaatatt gtcttttcct cccaactgtt caatcagcat atgaatctgc
     5041 tcggacagta ccatctgttc aggattcatt gtcgtttcca ttagaagacc actccactca
     5101 ctaatataat attggcgtaa ggtgtcattt cgcctgtacg aataaatgca tgacagttat
     5161 tcaactcttt tttcatttct tcatgtggga taaagctaat cggtgtatct ggtaacaata
     5221 atttgatttt ttccaatatt tccgggttca ttgtctttat ttcttccgcc aattcaatcg
     5281 attgtacctc aagttcttct aaaacgtttt ctaaaacaga taaaaaactt ggaagaccgt
     5341 ttgagacagc caaatctatt ttttcagtac caaaggggac aggcatgcct gcatccccga
     5401 tacttaattt atcaaagtgc cccatttgtg cgattactcg tgaaatatct gaattgatta
     5461 cttttccttt tttcatgttt gtttcttcct ctcattgtta attttagact gctgattgtt
     5521 ctaaagcttc gcggtaaggg atagaaggtt gcgcaccata agactgcaca gcaacagaag
     5581 aggctagatt tccataaagg atcgcttctt tcaagttact aaggtctggc tgaagtgtgc
     5641 tgcttaatgc gccaataaat gtatcacctg cggctgttgt gtcaacggca tccactttaa
     5701 aagcagggac gataccgtgt tctttttctg tatgatagaa agcgccggca ctgcctagtg
     5761 taatgattac tgttccaata cctaattcat ggagtttttc ggctgctgcg accaaactgt
     5821 tatgatctgt cactctgatt cctgtaatga tttctgtttc tgtttcattt ggtataatga
     5881 tatctgtctt ttcaagtagt tcagtaggaa tcgtttcacg agctggtgca ggattcagga
     5941 tcgttgtttt tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca tctaagctac
     6001 tttcaaactg agcaataata aagtcactgt ttgtaatcag ttgcttgttt tttaggactt
     6061 cttgttcatg gaaagcatta tttgcccctg catggatcaa gatactattt tctcctgctg
     6121 cgtctaccat aatcattgct tgtccggttg tggcgccttg gatttcagct atcccagatg
     6181 tatcgatgtt ttcttttgtc agtagatcta ataactgttg tccttcgctg tcagccccaa
     6241 cgcctccaat aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct tggttggcac
     6301 ctttacctcc gccagcgtga aaaatctcat gcgcatgcat cgtttcgcct ggcttgggca
     6361 tgttggttaa acgaagggta gtgtccatat taatacttcc aattactgta accgtgttca
     6421 ttttacaaac ctccaactat ctcatttatt ttgatgggaa aattattttt taaaacgttt
     6481 tagtaaaacg ttttaatagt tcgataaaaa tatagcacac cttgaaagct atttcaaggg
     6541 tgcagttgaa aatctttttt ctaatcgtac aggtaatctt ttttcttccc attctttttc
     6601 tgggaattgg atccgatcta acaataactt ggcagatgtt tgtcccaatt cgaagattgg
     6661 ttgtgcgatc gttgtcagtt tcggcttgat atactcgcac atatctatat tatcatagcc
     6721 aataatgctg tagtcttcag ggatgctttt tccagcttcc tctaatccgc gatataatcc
     6781 aaaagctaat tcatcattta aagcaaacac accagttgca gcagaatcaa gaagttctgc
     6841 tgtgatctgg tagccacctt gttttgagaa ctgtgtaggt aagaggatca gttggtcgtg
     6901 agggatttgg taaacatcaa gagcagattt aaacccctca atccgagcgt ggacattttc
     6961 cggtggattt tcaggataga caagggcgat cgtttgatgt cctagactaa gcaagtgcat
     7021 gcccgctagg taaccaccgc gaaaatcgtc tgttcgtacg gcatcactaa atccttcaga
     7081 ttttttctga tccaaaacaa tgaagggccg gccttgtttt ttcaaatttt cgttgattgc
     7141 atctgtagag acagcagaag tcgcgataat gaatccatcc acacctcgac gtgtgagctc
     7201 ggctagatat tcgatttctt tttgatgatc agaatcagca ttacataata tcgtgacgaa
     7261 attctgctta tacaagatat cctcaattcc tcgcatcagt gtactgaaga atggattcgt
     7321 gatatcagga acgagtacac caattgtttt cgtttccttg gtaatcattt gtctagcaaa
     7381 atagtcaggc tgatagccta attcttcttt ggcagctaaa actttctgaa cagttttcgg
     7441 actgaatttc tccgtatttc catttaatat caaggagaca gttgtgattg aaacgccgga
     7501 aagtttagcg acttctttga ttgtgatctt ttttttcacc atgttcatac tcctatctaa
     7561 atcctgtggt tattttccca tgaaatcaaa aagaagtaaa tgaaaatcac gtgtgccagt
     7621 acttatttgc tttgttggat agcttcgatt atttgtttcg ttgagcgtac tcgtgcagtt
     7681 ttcggaaata tcttttttat gaacaattga tgggattctt tgtcacgatc actgcaagca
     7741 tcttcagcaa gcacgatatg gtacccgtgt tgaaaggcat ctaatgcagt agcatataca
     7801 ccgtttgttg tcgctacacc gcttaaaatg atagtttcga ttccacgccg gcgaagttga
     7861 agatcaagag aagtaccaaa aaaagcactt ggattgtatt ttgtgatagt caacatatta
     7921 tctgtatctt tcaaagaatc cttcaaaagt aaatcagtga attctggagg tacagaagca
     7981 gtcattccac ccatatcagt ttgttgactc aaataaggga aagtggagat gtctacgttc
     8041 actaaaactt tcaaggcagc ggtacgtttc aatgttctta ctaacaaatc attgtttgtc
     8101 agaatagatg ctgcagtgtg tggagagagt gtaccgttat ttacgatctc tttttgcatg
     8161 tcgatcgcga caaaagcagt ttttgataaa tcaaaaatca tattttcctt taaatcattc
     8221 atgatgttcc ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata aatcgtttat
     8281 ttgtcaacta aattaaagga ttcctttctt tagtctagag aaattcagtg gtaaaataaa
     8341 taagtgtgag aaaacgcatt cgatgcatag atatagcgag aaatatcgtg aaaatggagg
     8401 aatcagagtg aagtatgtcg aagtaaaaaa tgaatacaaa cgatacaaaa tgggagaaac
     8461 gaccattaca gcaaatgacg atatttcttt ttcaattgaa aaaggcgaat tagtgattat
     8521 acttggtcca agtggagcag gaaagtcaac gattcttaat attttaggtg ggatggatac
     8581 acctgatgaa ggtcagatca ttattgatga cacagatatt gcgcaatttt cagataaaca
     8641 gcttactgct taccgacgaa cagatgtagg gttcgttttc caattctaca atctcgttcc
     8701 taatctaaca gcaaaagaaa atgtggaatt ggcaacggaa gtttctcctg atgcgcttga
     8761 tcctgtagag gtattgaggc aagtgggcct agctcatcga ttgaataact ttccgtccca
     8821 attatcagga ggcgaacagc agagggtttc aattgctcgt gcactagcta aaaatcccaa
     8881 acttctttta tgtgatgaac cgacaggcgc gctggatttt gaaacgggca agcaagtatt
     8941 gaaattattg caaaacgcta gcagacaaca cggcaatacc gttttgatca ttacacataa
     9001 ttctgcttta gcgcccatcg ctgatagagt cattcacatt aatgatgcta aagtaaggtc
     9061 tgttgaatta aatgatcatc cttcatccat tgatgagatt gtatggtagg gggattttat
     9121 gaaaacaaga acatatctta aagcaggttt tcgcgaaatt cggcaatcta aaggacgatt
     9181 cattgcaatc atccttattg tcatgctagg aacactttta tttgtaggtg tcaaaactgc
     9241 tggacctgtc atgcaaaaaa cgatggatca ctatgttggt acagcaggac tttctgattt
     9301 gcaaatcgtt tctactggtg gcttgacaca aaaagatatt gctcaagcag aaaagatccc
     9361 agatgcaaaa gtagaaacag gaaaacagat ctattattcc aatactggca aaaacgaagt
     9421 gattcaaata ttttcttata acaaaaataa caagcagaac aagctacaat taactgatgg
     9481 aaaactcccg gaaaaaccaa accagcttgt attggatgaa aaagcggaag atgaagggta
     9541 taaaatcggt gatatttatc agattgattc agatgaattg aaagaaaaag agtataccat
     9601 cacgggcttt gtccgttctc cattgttcat caacaatttg gagagaggat acgcgaacgt
     9661 cggaaatggt agcgtcgatt attttgtcta tttacctgaa tccgttttta aaacagatat
     9721 ttattctgtc ttatatcttg attttacaaa cgtaaagaat ttgaatacgt acagcaaagc
     9781 ttacaaagat aaaatggagc aaaatcaaga aaaggcagaa aagtatttac aagatcgacc
     9841 cgaagaacgc ttagaagagc tgaaaaaatc ggccaacgaa gaattagaac cggcacagca
     9901 aaaaatagcg gatggcaaag cacaaacaac gcaagcaaga acacggttag aagatgctaa
     9961 aaaacagtta gcgcaacagt cagcaactat ccagcagctt cctgaagcgc aacgaacaat
    10021 cgctgttcaa acattatctc aacaagaagc tcaacttgct gaacaagaaa ggcaattagt
    10081 tgagaaagaa cgtgaactgg cctctgcaga ggaagaattg aatcgaaatg cagaaaaaat
    10141 tgaaaattta actaaaccat catacctata caatgaacgt tcagaaaatg tcggttttca
    10201 agaatttggt gatctgtctg atcgtatcgc tgcgattgcc aatgtttttc ctgttttctt
    10261 tttctttatt gccgcattga tcacctttac gacaatgaca cgtatggtag aagaaaaccg
    10321 cagggaaatc gggacattaa aggcgttagg ttatacgaaa gttgaaatcg caaaaaaata
    10381 tgcaatctat gcaagtctag cctcagggat aggtattgtt ttaggaacga tattaggaac
    10441 gaatcttttg ccaaggatca tttttgagct gtctaacgag cggtatgata ttgggagtgc
    10501 gcttattttt tatgactggc cgccgatttt acaagcagct gccgcattct tcattgccgc
    10561 atttggcgct gcaatgatcg ttttattcaa agatttaaga gaacggcctg ctgcattgct
    10621 tcaaccgaaa gcaccgaagc cagggaaacg catttttctt gaatacatca ctccgctgtg
    10681 gtcacgcctt agctttaatc aaaaaatcag ttaccgaaat ctcttccgat ataagtcaag
    10741 aatgcttatg gcgattatcg gaattgctgg atgtgccggg ctgatggttg caggcgtggg
    10801 attgaaagat tctctaagtt ctgtttcagc caaacaattt ggaccgatca ttgactatca
    10861 agcaatcgtt acattggatt catccacgga agatagtcgt caaaaagcag cggatatgct
    10921 gcagaagcaa gaaaaagtaa atgagatcct agctattcat actcaaaccg tagaattgag
    10981 aaaaaaagga catgcttcgc aaagtctgac agtgatcgta ccaagtaaaa cagctgattt
    11041 ggatcgtttt atccatttaa ctgatcttga agagaaccca cgctccttgc ctaaaacagg
    11101 aatcgctatt acgcaaaaag tagcagaact atttgactta tctacaggag atcatatttc
    11161 gctttatgat gaagatcaaa aagaatggaa agtcgaagta aaagaaatcg tgaaaaacta
    11221 tctaggtagc tttatttata tgtcccctga atattatgct tcagttactg atcaatcgat
    11281 gatggataat gcatttttgc tgcgtacaga taagatgaca acaaaagaag aagaaatgct
    11341 ttccgagaaa ctgcttgcca cagacgctgt gacaaatacg tctttcatgt ccaaacaaat
    11401 cgaaacgcaa gaagaatcaa tggcgaactt agactcggtt gtcttaatct ttgtcgtatt
    11461 atcaggactg ctggctttta tcgtcttgta taatctaact aatatcaata tttccgagcg
    11521 tgtacgcgaa ttatctacaa taaaagtcct tggatttttt gataaagaag tcacgatgta
    11581 cattgttcga gaaaatatca tctttactct tttaggaatc atcggaggat ttggtgtagg
    11641 atatatattg actgacttca tcttgcaaca agcgtctatg gagaatgtca tctttccatt
    11701 agtgatcaca tggtgggctt atgtcttatc agcaggatta actatcgtat ttacggttat
    11761 tgtgatgatt gttacccatt tcaaattaaa gcatatcgat atgattgatg cgttgaaatc
    11821 gaatgaataa aaacagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa
    11881 cggcaaaata gtttttcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt
    11941 gaacaccatg aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca
    12001 aataaaatta gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt
    12061 ttgaacacca tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca
    12121 aatacatgtc tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat
    12181 aaattttaca aacgctttcg gttcagttat aataataaat gataacttgt atatataagt
    12241 tgtggtaggt aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt
    12301 catttaccaa atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt
    12361 aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct
    12421 aaaccctatt tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc
    12481 catcgatcct atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa
    12541 ataccggata gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg
    12601 gttcaaaaaa gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga
    12661 gccaacggat tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg
    12721 gaaatattat ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc
    12781 agtaagagag gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt
    12841 ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa
    12901 tgatggcaaa cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa
    12961 ccaagcaacg tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac
    13021 gatcgaaaat tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa
    13081 tttccatcat ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga
    13141 ttttgcagaa ttaaagcgac tatttcatac atggggagaa aagatgagtg agggtaacgg
    13201 ctggaatgct ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagc
    13261 tatcgatcag tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg
    13321 tgggacgccc tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc
    13381 gatttccgac tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg
    13441 attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac
    13501 acccatgcaa tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa
    13561 agttggaggc aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat
    13621 tttgtcctat tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg
    13681 agattatcat gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga
    13741 aggacagcag ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc
    13801 agaaggattc acagaagcca atattcttat cgataattat tccgatactg ttcttcatca
    13861 aagtctggag ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac
    13921 aaaacgtcgt tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata
    13981 atcttcttca gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta
    14041 gcgttgtttg aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg
    14101 aataccaaaa ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa
    14161 gatcgtaatc ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc
    14221 gtggagaagt tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc
    14281 ttggaatgca atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat
    14341 acataacaac ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa
    14401 acaaacccag tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga
    14461 acccaattgt tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg
    14521 gacaatcgct aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac
    14581 aattggacca gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat
    14641 ccgttgtgcg ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc
    14701 gtcaatagaa gcgaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg
    14761 tcggagactc tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc
    14821 ctgttcttca agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat
    14881 cacataaacg ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac
    14941 tggttccaaa agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat
    15001 aatgattccg acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc
    15061 aagtaagata actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa
    15121 acgattcgtt gtatctgttt cttttttcac tctttttcct cctttatcta taacttcatt
    15181 atagacaaaa gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat
    15241 cattcatata aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca
    15301 tatgataaat taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg
    15361 gaacattcaa atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta
    15421 tattggccat gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg
    15481 aggacagtaa agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa
    15541 aagcaatcgt cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc
    15601 acgattatga accttctgct aaagacatgg caaagatcca agatacagat atctttgttt
    15661 ataatgatga aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca
    15721 atgtccatac gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac
    15781 atgatcatga ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat
    15841 ggctagctcc aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag
    15901 cttacccaga aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc
    15961 aagcactcca tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca
    16021 cgcaacatgc tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg
    16081 ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg
    16141 ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca
    16201 gaacgttagc aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga
    16261 cgaatgaaca aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag
    16321 cgttgaaaaa aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag
    16381 aaaagagcgt ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag
    16441 attgggcagg aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg
    16501 tttttgaata taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt
    16561 atacgaaggg ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat
    16621 ttacgtttaa gaatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct
    16681 tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa
    16741 gtggcgcttt tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac
    16801 attttcattt atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt
    16861 ggccaacatt ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc
    16921 actgataggc aaaaaagaac cgaagggttt ggatcggctg gtcatttagt cggtccaaac
    16981 tattcggttc tttttatgaa atcaatttct ttaatccacg gatagaggct gggatggtct
    17041 cgccgtttgc caacgtgact tctctggttt ttttattaat cgaccgcaca ttttgtgtat
    17101 tgataacata ggatttatga caacggataa aattccgatg cattttctcg atttctttga
    17161 tcttctcata gaattgtaat tgggcatgag tcaaatggac ttccagttta tgtggcgctc
    17221 ctagcgaagt agaaaaatat tggatgtctt tgatcggtaa aaatttgatc tcatcattaa
    17281 actctatttt gaagatatct ttttggattt gggacatttg aaaatcttcg aaaattttgc
    17341 tcaggatatg ctcgacattt tcttttaaat cataaagatc ttctttgacg atataatcag
    17401 tcggttcgat gttgcttttt aagattttca tcgacatttc cgtatgggaa gtcacataga
    17461 ttatttttcc taaaggatct aattcacgga tcaatttacc gatcgctaca ccatccattt
    17521 cactgtcttc tagttcgata tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg
    17581 ctgcattgag aagttcttcc ggatcagagg tagaaatatg gacataactg tctaagtttt
    17641 caatcatgat tctatttttg atcaccgttg ataaaacttc taattgtttt ggatcgtctt
    17701 cacaaatata aatgggaatc atatcattac tcctttattt tatttgtata taaatcgttt
    17761 gagaaaaacg ctggttttca attgatgttt caaggaaaag ataaggattg gtctttgtca
    17821 attcatccaa gataaacaag ccaaggcctt cgttttttgg atttgttttt gttgagaacc
    17881 ctttttgttt caactggttt aacggtaatg tatttgttgt tgtattgatc acacgaatca
    17941 tcacggaatg attcttcttg agtatggtca attcgatttt tttcttcttt gcagcactag
    18001 ctccttcaat cgcattgtcc aagataatac caagcatacg tacaaggttt accgtatgtt
    18061 tcgtatccag ttcgattggc tcaggaaaat gaagctggac gatcaaccct ttttcctgtg
    18121 ccatcatgag ttttaatgac agtaaactac ggatctcagg aactttcata ttatccaaca
    18181 tacttagatt ggccggaaaa aggttcatca tctggcgtgt aggtaatatc gtttgatcat
    18241 aataagaccg taatccttct aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca
    18301 gattttggta atcatgtttg aattctctca agtcttgata gtttttttct agatctttga
    18361 tataatcctg ttgcatcctg acttgttgct ctaataactg atcagctaat ttcttattag
    18421 agattttcca ataataaacc aagatgatca ggccaacaca cagatataga atgaaaaaac
    18481 tcgtattcaa agctaagaca tcaggtgttt caccagaaaa acgggtataa aaaataatga
    18541 tatagtatgt aaataatatc aatgtcccga tcgtaccgat catacggcgg tccacagtgt
    18601 gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg
    18661 ataagacaat gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa
    18721 caaaaaaatc gccgatgctt gttaagtgat ctcctaaaag cgacgtcaat aacccaaaag
    18781 gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa gaaaaaagta agcatcagca
    18841 ttcctatagc aattagagca gtaaaaatcc ctaaaatcga atagacccat ccaatcaaaa
    18901 tacaagaaat cagaatgact cccgcaaaca gatatttgta tcggatgtga tggtcaatca
    18961 ataagataca aattgtgtaa atcaaatatt gcaacgctat ggaccataaa aacataaagt
    19021 acagctccca ttgattttta ccaaattata gcacaattgg cgtattattt ttttaatttt
    19081 tcagggattt tgaattcaga actcatacca aagcagcagc cagatacaaa aaagtcagat
    19141 aagttagtta attgtttaat gattgttttt ttcatgaaag attcctccaa aattcatcaa
    19201 gtagtttgta ataggaagca acgtagctat ttctaaaaca atcccaagta gcaaataggt
    19261 agaaaagtaa gaagctgaga aaaataacgt aatgagagaa aaaattagaa acatgctgat
    19321 cgccttcttt aataatgggg gacgtttctc gggttcaatg ggattgattg ctgtacctct
    19381 tgatccatag cgaagcagta acaaaaagca gctgattaac aaaaagacca tcattgggaa
    19441 ggagatagtc aattgcagat gtttcatcac caagggaacg ccccataaat aaagtagtgt
    19501 ccacagcaga caaacgaatt cagagtcgga atgcaatcca taagcatatc tgcggatgca
    19561 gatataagaa agaaaggtga ttcctgtttc cgcccatgag cctgtcacca gagaaaagac
    19621 tagaacgacc gctagttttc caagattatt caataaaact tctaagacaa attttacttg
    19681 gacatacacc atatcttctt ccgatttgtc tccgctgaat aaggcttcta ataatttgtt
    19741 cacaacttta tctttcatct catacgtacc tccaccacaa attttatgct aaaaaaaata
    19801 aaaaacgtct tttgtttcat aaaaagtggt tttgacatca aaaccgtcat gatctcctaa
    19861 aaaaatcttc gttttccaag aaccttacca aaaaaagtga cagttttgat acgaaaagta
    19921 ctttttatga caagtttgtg ctttttcgag tgatttatgt taaaaaatta ctgaccttat
    19981 aaaaggtgat ggagggattc ttatggaaaa tgaacaattg tttgaagtcg gcgaaacata
    20041 tgcatttaaa tctgatttat ttgaatcacc aataaaagga aaaatagaaa aaatctatga
    20101 aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa gacaatgata ccgctcataa
    20161 tctgcactgg cagttagttg tacgcaaatc aagtacaata gaagaataaa aacagataaa
    20221 atgttgagag cataaagagg ttgtgagtct cccgtttagc tctgagttac aaggaataat
    20281 gttaaaaaac agcttttcat gttttatgaa tattgccttg tgaccgaggt gttggcagga
    20341 gaatactgtt tatttgaaaa aagggctgcg acaagacttt tgtcacagcc tttattttca
    20401 tgaataggag ttacttttgg ctacttttag agaagtagct tttaaggagc agataaagtc
    20461 aaggctttta tttctataaa tgaagtagta aaatgaagaa cagattttat tacaggaagg
    20521 atgccgataa ttgtgatcat tgttgcagga acgatcggtg cagggaaaag cacactaact
    20581 gaaatgcttg cccaagattt agaaacaaaa cctttttatg agaacgtgga agataatgaa
    20641 gtattgccgc tattttattc taatccagaa aagtatactt ttttattaca gattttcttt
    20701 ttgaacaaac gctttttggc aatcaaagat gctttcagcc atgatgacaa cgtactagac
    20761 cgctccatct acgaagatag tatgctgttt catttgaatg cagatttagg gcgtgtttct
    20821 gaagtagaag taaagcagta tgaaggtctt ttagaaacga tgctgaagga gttagaagaa
    20881 atttcgccac aaaagaaacc agatctactg gtttatattc gtgtatcctt tgaaactatg
    20941 ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc tagaacaaga tcctgaactc
    21001 tattcctact ataaagaact caatcgacga tacgaagagt ggtatgaaca gtttgatata
    21061 tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg tagccgatcc ggcatgtgga
    21121 gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa tgacagaaga ggcagatgcc
    21181 cagtcgatcc gctacactac aaataacccg aaagcaaagg gaatctttac aactaaataa
    21241 tataaaaact aagcaaattg ctcactcgca gatatttctc tgtgagcagc atcttgctta
    21301 gtttttttga ttcattgaga tcaaatagtg aaccgattcg tatgataaaa ttgccataaa
    21361 tgaaatgtct gagtgagggc catgatcaaa aataaaatgg aaacgatcaa aatcgaatgg
    21421 gcgccaaaca gatgatgaca gaaaacaagg acaatagctc ctgcgataaa ggcaagaacg
    21481 acacttagat agtggaaaaa tcgatcaaaa tctgcttttt ctttagaaat acaataatca
    21541 tataagttcg ttgctacccc ttttaaattt ccagttgtaa ataaattagt gtaggcacgc
    21601 ccgttgattt tatcaaaagc tacccactgg atcgatgcag taaaagcaat caaaatcgtt
    21661 actaatgcgg gatgctcatg gacgggtgtc aaactaacaa agaaaaaaat cactgcttca
    21721 taatatagaa caaacaagcg ccagaaatgt ttttcttttt ttctaaaata atcaattaga
    21781 tactttgtta gaagaatccc taagaaaaag aaaagggtag acaatacttt tttaccaaca
    21841 cttagccatt ctccgtcaaa tgcttggatg atcgatagaa tgatattgcc agtttgagca
    21901 gaggcaaaag caccatattg gatataagta taactgtcca tggctcctcc gataaatgtc
    21961 agcaaaagtc ctattgtacg cccctcgtga aaggaagtct gcattttttt catcaccttt
    22021 tatgaatatt tttgtcttcc ttatcttagc ataaacgatt gaaaaaaaag gaaaaatttt
    22081 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
    22141 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
    22201 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
    22261 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
    22321 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
    22381 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
    22441 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
    22501 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
    22561 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
    22621 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
    22681 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
    22741 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
    22801 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
    22861 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
    22921 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
    22981 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
    23041 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
    23101 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
    23161 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
    23221 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
    23281 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
    23341 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
    23401 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
    23461 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
    23521 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
    23581 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
    23641 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
    23701 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
    23761 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat
    23821 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
    23881 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
    23941 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
    24001 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
    24061 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
    24121 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
    24181 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
    24241 agcagaacca gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
    24301 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
    24361 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
    24421 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
    24481 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg
    24541 aaaagggagg tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat
    24601 actattatca aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag
    24661 ttattttgat tttttttata ttttattagc taataattgg tattttaaaa gaaattactg
    24721 tagaattgga ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag
    24781 ctgtacttcc tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga
    24841 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac
    24901 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
    24961 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
    25021 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
    25081 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
    25141 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
    25201 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
    25261 atcaatttgt tgacttagaa gaacgcgatt taagtacaga agaagcagaa gaattatgca
    25321 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
    25381 ttgcgattga cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata
    25441 ccaatctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
    25501 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
    25561 tgagcatttc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
    25621 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
    25681 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
    25741 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
    25801 tgttgacatg ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt
    25861 gaagcttgat tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag
    25921 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
    25981 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg
    26041 aatcggggaa cactcctgat gaagtagacc tttgaaaact gaacaaagta agacaaacca
    26101 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca
//
LOCUS       DACDOA010000031        25567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDOA010000031 DACDOA010000000
VERSION     DACDOA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.031"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..280
                     /locus_tag="IWI39_001174"
                     /pseudo
     CDS             152..280
                     /locus_tag="IWI39_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
     gene            394..633
                     /locus_tag="IWI39_001175"
     CDS             394..633
                     /locus_tag="IWI39_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208290.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            745..939
                     /locus_tag="IWI39_001176"
     CDS             745..939
                     /locus_tag="IWI39_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208291.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1025..1696
                     /locus_tag="IWI39_001177"
     CDS             1025..1696
                     /locus_tag="IWI39_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9208292.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1749..3725
                     /locus_tag="IWI39_001178"
     CDS             1749..3725
                     /locus_tag="IWI39_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9208293.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3740..4492
                     /locus_tag="IWI39_001179"
     CDS             3740..4492
                     /locus_tag="IWI39_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9208294.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4508..5263
                     /locus_tag="IWI39_001180"
     CDS             4508..5263
                     /locus_tag="IWI39_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208295.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5276..5536
                     /locus_tag="IWI39_001181"
     CDS             5276..5536
                     /locus_tag="IWI39_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208296.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5533..7623
                     /locus_tag="IWI39_001182"
     CDS             5533..7623
                     /locus_tag="IWI39_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9208297.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7650..8114
                     /locus_tag="IWI39_001183"
     CDS             7650..8114
                     /locus_tag="IWI39_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208298.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8111..8353
                     /locus_tag="IWI39_001184"
     CDS             8111..8353
                     /locus_tag="IWI39_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208299.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8371..8673
                     /locus_tag="IWI39_001185"
     CDS             8371..8673
                     /locus_tag="IWI39_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208300.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8743..10806
                     /locus_tag="IWI39_001186"
     CDS             8743..10806
                     /locus_tag="IWI39_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208301.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10806..13418
                     /locus_tag="IWI39_001187"
     CDS             10806..13418
                     /locus_tag="IWI39_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9208302.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13415..15946
                     /locus_tag="IWI39_001188"
     CDS             13415..15946
                     /locus_tag="IWI39_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208303.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15996..16328
                     /locus_tag="IWI39_001189"
     CDS             15996..16328
                     /locus_tag="IWI39_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208304.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16329..16922
                     /locus_tag="IWI39_001190"
     CDS             16329..16922
                     /locus_tag="IWI39_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208305.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17085..19109
                     /locus_tag="IWI39_001191"
     CDS             17085..19109
                     /locus_tag="IWI39_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9208306.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19109..20308
                     /locus_tag="IWI39_001192"
     CDS             19109..20308
                     /locus_tag="IWI39_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9208307.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20324..20968
                     /locus_tag="IWI39_001193"
     CDS             20324..20968
                     /locus_tag="IWI39_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208308.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20979..21785
                     /locus_tag="IWI39_001194"
     CDS             20979..21785
                     /locus_tag="IWI39_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208309.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21807..22037
                     /locus_tag="IWI39_001195"
     CDS             21807..22037
                     /locus_tag="IWI39_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208310.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22551..22859
                     /locus_tag="IWI39_001196"
     CDS             22551..22859
                     /locus_tag="IWI39_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208311.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22861..23283
                     /locus_tag="IWI39_001197"
     CDS             22861..23283
                     /locus_tag="IWI39_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208312.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23303..24892
                     /locus_tag="IWI39_001198"
     CDS             23303..24892
                     /locus_tag="IWI39_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208313.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24904..25239
                     /locus_tag="IWI39_001199"
     CDS             24904..25239
                     /locus_tag="IWI39_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208314.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25380..>25567
                     /locus_tag="IWI39_001200"
     CDS             25380..>25567
                     /locus_tag="IWI39_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208315.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9542 a         3961 c         5257 g         6807 t
ORIGIN      
        1 agatcaaata aacaagaacc gtaggaacta cggggatagc ttggtaaata agaggcacct
       61 ctgttagtaa agtaatacgc tatcaagtat gctctcttcc caagaagctc ccacttcaag
      121 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
      181 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      241 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      301 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      361 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      421 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      481 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      541 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      601 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      661 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      721 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      781 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      841 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      901 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      961 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
     1021 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
     1081 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1141 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1201 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1261 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1321 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1381 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1441 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1501 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1561 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1621 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1681 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1741 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1801 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1861 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1921 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1981 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     2041 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     2101 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2161 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2221 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2281 aaaaagcaaa accaatggtt gtgattgcgc caatgacaga taaaacagga tcaggatttt
     2341 taaaaaatat tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga
     2401 aatttggtga cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg
     2461 agttatacaa gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc
     2521 aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt
     2581 cggaagttgt agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag
     2641 acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa
     2701 gtgacttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa
     2761 atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga
     2821 ttcatattcc aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa
     2881 gtgaaacttc tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt
     2941 atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag
     3001 aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga
     3061 ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt
     3121 ataacatgta tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt
     3181 tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg
     3241 ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca
     3301 aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg
     3361 gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag
     3421 caaatgcagc gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg
     3481 aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag
     3541 agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa
     3601 acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa
     3661 tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag
     3721 cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa
     3781 atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga
     3841 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     3901 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     3961 tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa
     4021 aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg
     4081 taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt
     4141 accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     4201 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     4261 cttagaatca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     4321 caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     4381 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     4441 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     4501 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     4561 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     4621 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     4681 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     4741 tcacgttcaa aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga
     4801 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     4861 tgttaaggat ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact
     4921 atccagtgat caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac
     4981 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     5041 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     5101 ttctgataaa aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc
     5161 gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga
     5221 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     5281 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     5341 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     5401 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     5461 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     5521 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     5581 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     5641 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     5701 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     5761 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     5821 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     5881 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     5941 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     6001 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     6061 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     6121 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     6181 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     6241 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     6301 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     6361 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     6421 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     6481 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     6541 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     6601 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     6661 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     6721 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     6781 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     6841 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     6901 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     6961 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     7021 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     7081 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     7141 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     7201 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     7261 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     7321 acgctgaagt gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa
     7381 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     7441 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     7501 cctaacttgc cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca
     7561 gcggtcagct tactattact tgtgttagca acttttagtt atgcggttct gagatacatt
     7621 taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt aataatagta gttacttcct
     7681 tgattgccat gattgttatt ttagtcggat gtagtaatga aaaaaataaa caaactaatc
     7741 aagataatgg cgtattgaaa tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt
     7801 ataatttgaa aattatagac gagactacct gggaatactc tgagagtgta tggcatccag
     7861 atcctgtaca aataacagtc aagaggcaaa aagactataa aggtttagaa agatacaaaa
     7921 tagtagattc agctggtgtt agggaattta taaacaaatc tgattcttta tttattgttg
     7981 ttccctatga gaagaatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaaa
     8041 agcgaacgaa agagaaattg attcacgatg gatcacaatc caataaatat aagttacaaa
     8101 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
     8161 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
     8221 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
     8281 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
     8341 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
     8401 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
     8461 agtttaaaaa acaatctgat gatttttgct gctgctgcgg ccgttgcttc cattgttatt
     8521 gggggtcttt tcttcctatt tggacgaaga ggagctgaaa atgggaagat gcacgtaggc
     8581 tatgtattga tgggggtagg agttatttta gcagcagctt ctattgtggt ttcgttattt
     8641 ggattgtttg gacaaacacc tcaaggatta taagaaagta aaaaagtagg agtaagaagt
     8701 agtcgatact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgattgca
     8761 ggcaatacca aaacagataa agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt
     8821 acttcttttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
     8881 gtgttaggga aagtactgga acacgaaagt cgatcttctg ttaagttgac tctcacgtta
     8941 catgacacag aaggaaaaat agagaaagct ggtacagaat acgtttttgg cccactggat
     9001 tatcaaattt cagaaggttt ctatcctaca ttgatggatt taatccgcct tgatgtgttt
     9061 aaagaaacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa
     9121 gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa
     9181 gtaaatgaag aagtaccatg ggaacgacca atatttccaa agaaggagtc aaaagaaaaa
     9241 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
     9301 gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
     9361 gagacggccg aagtagaagt tgatccagtt gaagcgaatg aagagtcaac atggactgaa
     9421 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaata
     9481 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
     9541 tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc
     9601 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
     9661 cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat
     9721 ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg
     9781 acggaacagt cgtctaattg ggagaaagca aaagaggaac taaataaaga agaacagcga
     9841 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
     9901 tattccttgt gtgtagcaaa gatcaataat tttactcaga cgaatcaaga agcgttatcg
     9961 aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga attttaccga
    10021 ggtttgcgta cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta
    10081 tatcaaacaa cgactgaagt tcttatggaa aaatacgaaa agatccagca agaattacaa
    10141 gtgcaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    10201 gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa
    10261 caggatcttg cggaacaaca aatggcaata gaaaaacaac ggttaaacca agaaaaggaa
    10321 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagctcag
    10381 ttaaattatc tagcacaacc tcaaccaacg ccttcagtag ctgctgttca aaccccaatg
    10441 aacaaccaaa caaatctaac tccttcacca cagcgttcct ttaacggatg gatggtggga
    10501 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    10561 aatcaatctt cacaggctga ttctgtggcc acaacgacgt caatcaagga agaagcccaa
    10621 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    10681 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    10741 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    10801 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    10861 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    10921 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    10981 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    11041 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    11101 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    11161 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    11221 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    11281 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    11341 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    11401 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    11461 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    11521 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    11581 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    11641 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    11701 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    11761 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    11821 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    11881 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    11941 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    12001 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    12061 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    12121 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    12181 taggctataa aggattttgt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    12241 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    12301 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    12361 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggagaaaagt aacttattag
    12421 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    12481 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    12541 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    12601 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    12661 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    12721 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    12781 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    12841 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    12901 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
    12961 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    13021 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    13081 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    13141 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    13201 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    13261 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    13321 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    13381 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    13441 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    13501 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattta gttggatttg
    13561 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    13621 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    13681 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    13741 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    13801 atttatggcc atgatcttga tttttggtgg gagttcttta atcgatgaag ggttaaatat
    13861 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
    13921 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acattactag caactgaccc
    13981 gaataaagta gaatccctgc caacagacaa aagaaatgct ttaacagcga aaaattttaa
    14041 aacagcgaat atcaatggga ttattactcc taaacaagct aaagaaatgg cggataaaga
    14101 taagagtggt ttatcagatt tacaaaagga tcggttaaga gcgttacaat accgattgga
    14161 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    14221 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattatct tagctggtga
    14281 atcttctttg gcggtggcct atttatttat cctatttacg atcgttactt gcattattga
    14341 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggcca
    14401 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    14461 tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc atgcggcagg
    14521 aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg cgctattcaa
    14581 aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa acggcagtaa
    14641 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    14701 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    14761 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    14821 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    14881 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    14941 tgaagatctt acagaaaaag ccaaagacac cttagaagag aaattagacg gcacgagagc
    15001 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    15061 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag ttaatgcagc
    15121 agtaaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    15181 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    15241 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    15301 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    15361 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    15421 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    15481 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    15541 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    15601 agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc aatcaactgc
    15661 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    15721 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    15781 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    15841 atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga acaatgcatt
    15901 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    15961 aggcctttta tttatatgaa aaggagaaat caattatgga taaatatata aacatgaaaa
    16021 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    16081 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    16141 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    16201 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    16261 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    16321 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    16381 gaaaaccaga agagcgctct atcttggact tatttccgta taaatcctgt acagaagagg
    16441 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    16501 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    16561 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    16621 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    16681 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    16741 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    16801 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    16861 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    16921 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    16981 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    17041 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    17101 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    17161 atcgggaaat agaaatttta aaatccaaag ggtatgattt agatttttta aaacaagtga
    17221 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    17281 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    17341 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    17401 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    17461 cgactgaccg aaataaaagc gcagaagaat acctggaact agcggaatat gctcgtaaat
    17521 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    17581 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctaaaa gggatggatt
    17641 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    17701 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    17761 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    17821 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    17881 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    17941 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    18001 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    18061 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    18121 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    18181 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    18241 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    18301 ttacttcctc aaattcgcgt tcaaatacgg agacgcaagt cacaggtaaa gatccagaag
    18361 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    18421 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    18481 cgtatggcat gatttttgat gggcatagta cgattcgaca ccttgaccaa caacagttgg
    18541 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    18601 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    18661 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    18721 agtgccataa tgcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    18781 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    18841 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    18901 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    18961 tgggtaaatc aatgacgaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    19021 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    19081 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca
    19141 gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg gtgtttggta cttctgcagg
    19201 tttgattact atattgatca ctgtgtttgt gacccttttt ctaatacttg cggcaggttc
    19261 tattgataat tctgattcaa gtagtggtgg cgaagctttt acaggagaat attcggaagg
    19321 cttacccatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata
    19381 tgctgtaggt gcagcagtga agtataaatt attaccaagt gtgatccttt cgcaatacgg
    19441 ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaatt attttgggat
    19501 tacgtggttt gatgggtgtc tgtttcctaa aggaactgcg cgtggaattg gtggcattga
    19561 aggtggctgg tacatgaagt ttcccaactc aaaagctgcc ttctcctatt acggctttat
    19621 ggtagccaca caatcaaatt ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt
    19681 attgatctta ggacgaggag ggtatgccgc cgcaggaatt actgaagaca gtgcctatta
    19741 taaaaattgt atgtctatta ttaatacaaa taaattgatg gagtacgatg agtttgcgat
    19801 taaacactgg agagaaggcg gcggaactgg tggtacgatt acaggaagct ggggcaatcc
    19861 attcccagga agtagcttag ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa
    19921 tccaggagga gaattccgac ctaacgggtt ccatgatgga ctagactttg gttcagtcga
    19981 tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc
    20041 tggtatttca ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt
    20101 gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa
    20161 agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat
    20221 ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt
    20281 accatttttg aatagcagca aaaaatagat caggagaaaa aatatggaaa ataaaacctt
    20341 aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg
    20401 gcagcttaaa gaagaacaag agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc
    20461 gtctgataag ctcaaaaaaa attcttctga ggcacaggcg aagatcgaca agttaaatca
    20521 aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc
    20581 aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat
    20641 tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga
    20701 agtgatcgac aaattaagtc ctgcagacga tgatcaccaa catgacgact acggaagtta
    20761 ctcttttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca
    20821 aaattttgtc gtatttgttg attatacaat cggcaatcct caatttaaag atgtcaaacc
    20881 acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt
    20941 cacagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa
    21001 gacaacgttt taaaaaacga aaaagttatc ttagatggct ggggatcttt atgattctcg
    21061 gctttttgat tcttgctagt ccgatttaca cacataaaaa ggtagacata ccagatgaag
    21121 taagcacgaa cgcattttta aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag
    21181 cgaaccagca attagttttt gaatttgcga tcgacgatga agatcagagc ttattaaaag
    21241 aactagctaa tcttaattat cgtgtagaag taaagacagc taaaggggat tatcaagcaa
    21301 taaaagccaa agtgatcaag gtgtctgatg attattttgt agtgaagtta gcacatgtac
    21361 cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg
    21421 atatgcaaga accacaagat ctgatttatt acgttcacga agataaggtt aaagatcaag
    21481 tgaaagatga agattatgaa caacatgcga tcaattacaa agtgaaagga tacaaaaaag
    21541 aacaaaaagc agcacaaaaa caaatggaat ccttccaagc aaccattgat ttgaatgaag
    21601 aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata
    21661 ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg caaatgaaag
    21721 atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga
    21781 tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt
    21841 tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
    21901 gtcgagaagt gaatttatct aatacaacaa tatctgagtt gaagaatggt cgaaagaatt
    21961 tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta
    22021 cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct
    22081 tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac
    22141 gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga
    22201 tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt
    22261 tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta
    22321 aatacacaaa taaccttaat ttataaagag cataaggaaa cgtgggaaca ctttttaaaa
    22381 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataattgtgg gaacaaaata
    22441 tttaaaaata ggatagtgaa aggatagcaa aggatagtaa aaggatagaa gcaggatagc
    22501 gaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
    22561 gaataagaaa tgtagatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaactaa
    22621 gtaaccgaac gggtaataaa tggagtcgta acgattattt aaagctactc gttgaaaatg
    22681 attttgatcg tcctttgatg gaatataaac aagataaatt tgatcaatta ttaaatcagt
    22741 taatagataa attttctttt gttcaaagtc ataatacaaa agtattagaa gaatatattt
    22801 gtcagaataa tcaaattatc gaacttttaa ttgaacaaag tagaggagga gaagtttaac
    22861 atggccaaag ttatagacaa gcatatttat ttttatgaaa cacatataga tgttctagat
    22921 gaaattattg caactcatgc agaggttaaa aactatagtg aagccgttcg gtttctatgt
    22981 atgaattatg attcagaagt ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgca
    23041 atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga tctccatttg
    23101 aaagatacag ggattttaat tggtacaaat tcaactgttt accaagaagc aaaagagcgt
    23161 gtggaaggca agataaaagc aaatcaaacg aagaaatatt ctcctaagat aaagaaaaca
    23221 ataagcgaat cagtgactac aaaaagtgaa gtatttgatc caacaaggtt cttaaaacga
    23281 taaaaattag aaggagaatg tgatgagtaa atcacctgca gtcattctca cttgtcaatt
    23341 taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc
    23401 gctggaagaa aaggaacgac gtacgcccga ggaagaacag gaactgcgtc taatcaagaa
    23461 tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta agacgggtaa
    23521 agaattcagc gaaacgtcga atgaagcgca atcgttgatg aaacatggaa tcaattttga
    23581 caagttagaa gaacaagatt ttaccaaata tctttcttat atgacacgtc actatgcgtt
    23641 agaacaaaag aaagtactga cgcatagtga acaaaaagaa taccaacgct tgaaccaagc
    23701 aataaaaaaa tatcaaacag agccacacaa aaaagcttca ggaaatcttc ctggagtttt
    23761 ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaagc aaaccttcca
    23821 aagaggacaa caaaaaggct caatcattta tcaagatgtt gtctctcatg atcattcgta
    23881 tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag aaggtttaaa
    23941 agcagctgga agaaaaatga tggagacatt gttcgagaaa gagcaactga atgaaacagg
    24001 ctactggatg gcaacgattc accgaaatac caaacatatc catattcatt ttgcagttgt
    24061 tgaaaaaaaa aatacgcgta aaccatacac tgagattgaa aatggtgtga gttatatcgt
    24121 accgaaaggg aagagaaggc aagcaacatt agacgaaatg aagactaatt atgtacatga
    24181 gttagaacgt ttttcttatg aaaaaaatcg aatgggtatg gagaaacgaa atttattgac
    24241 acggaaaagt gatttgcgta acaccttaac caaagtagtg aaagagccag tacgttatga
    24301 tcgacacgca atgagcttat taactgaagt atataaaaac ttacctaaaa agcgttcgga
    24361 atggcattac ggggatgaaa atcgaaccaa actttcacca tacacaagag agaaactcga
    24421 tgagttaacc caatatgtgt taacaaaaga acctggatat caagaatata tacagatttc
    24481 aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga
    24541 tgcagaaaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca ttctaacaga
    24601 actgaagaaa caggatagtc aaataaaacc tttaatagaa aggatggatc agatgcaatc
    24661 tatttacaat gaacgatcct ttcgaacact taaacagttg ccaacgaatg aatacaaata
    24721 tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcaacg
    24781 gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa
    24841 agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt aggaggaata
    24901 gcaatgaaac aatatttaga tcaatggaaa gttatagaag gatcattaag agaagaacga
    24961 atcgaacaac ttcccgattg tttagaaaaa gagcatttgt ttcagataag agaaatgttg
    25021 ataaacgaac agtttgatcc aaatcaattt ttagtcgttg agtacccagc tactggtgtg
    25081 tactgttgta atcatgtgaa gggtgaaaaa tattttatca ttcaagaata tgagggcaag
    25141 ttagcgcctt attatactac ttgggaaatg aatgaagagg ggatcaataa cttcccgtgt
    25201 aaaagcattg aagagtctat ttcactcaca gaatgttagt tgttaagtga ataaaagaag
    25261 taaaagagtc ctgaacgtga ttgtccagga ctcttttttt tcactgaatt gttttccgcc
    25321 aattcattga gtggtgcgat ttccgtctac attatatcaa aagaaaggag gaagaagaaa
    25381 tggcttattc aacgattgga gttgttgttg aaaaaagcag agacaatctt gtttttgtga
    25441 cagaaattca aacaggcaga gcttttgtag tcacagacaa agcggcaaaa gcatatcaaa
    25501 gtggcgatat tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg gcagaagatt
    25561 atccatt
//
LOCUS       DACDOA010000032        24662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDOA010000032 DACDOA010000000
VERSION     DACDOA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.032"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(233..1081)
                     /locus_tag="IWI39_001201"
     CDS             complement(233..1081)
                     /locus_tag="IWI39_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9208316.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IWI39_001202"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IWI39_001202"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9208317.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IWI39_001203"
     CDS             complement(3582..3761)
                     /locus_tag="IWI39_001203"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9208318.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IWI39_001204"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IWI39_001204"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9208319.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IWI39_001205"
     CDS             5909..8188
                     /locus_tag="IWI39_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9208320.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IWI39_001206"
     CDS             complement(8281..8928)
                     /locus_tag="IWI39_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208321.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IWI39_001207"
     CDS             complement(9107..9826)
                     /locus_tag="IWI39_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9208322.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IWI39_001208"
     CDS             complement(9957..11108)
                     /locus_tag="IWI39_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9208323.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IWI39_001209"
     CDS             complement(11325..12065)
                     /locus_tag="IWI39_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9208324.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IWI39_001210"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IWI39_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9208325.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IWI39_001211"
     CDS             complement(12449..13042)
                     /locus_tag="IWI39_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9208326.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IWI39_001212"
     CDS             complement(13026..13673)
                     /locus_tag="IWI39_001212"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9208327.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IWI39_001213"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IWI39_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9208328.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IWI39_001214"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IWI39_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9208329.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IWI39_001215"
     CDS             complement(15119..15646)
                     /locus_tag="IWI39_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9208330.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IWI39_001216"
     CDS             complement(15826..16611)
                     /locus_tag="IWI39_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9208331.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IWI39_001217"
     CDS             complement(16604..17473)
                     /locus_tag="IWI39_001217"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9208332.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IWI39_001218"
     CDS             complement(17474..18853)
                     /locus_tag="IWI39_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9208333.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IWI39_001219"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IWI39_001219"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9208334.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IWI39_001220"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IWI39_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9208335.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IWI39_001221"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IWI39_001221"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9208336.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IWI39_001222"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IWI39_001222"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9208337.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IWI39_001223"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IWI39_001223"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9208338.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IWI39_001224"
     CDS             complement(22811..23035)
                     /locus_tag="IWI39_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9208339.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IWI39_001225"
     CDS             complement(23088..24050)
                     /locus_tag="IWI39_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9208340.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IWI39_001226"
     CDS             complement(24060..24500)
                     /locus_tag="IWI39_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9208341.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6774 a         5466 c         4277 g         8145 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tt
//
LOCUS       DACDOA010000033        24264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDOA010000033 DACDOA010000000
VERSION     DACDOA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.033"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..331)
                     /gene="trxA"
                     /locus_tag="IWI39_001227"
     CDS             complement(17..331)
                     /gene="trxA"
                     /locus_tag="IWI39_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9208342.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(418..2778)
                     /locus_tag="IWI39_001228"
     CDS             complement(418..2778)
                     /locus_tag="IWI39_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9208343.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(2893..3441)
                     /locus_tag="IWI39_001229"
     CDS             complement(2893..3441)
                     /locus_tag="IWI39_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9208344.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(3500..3937)
                     /gene="zapA"
                     /locus_tag="IWI39_001230"
     CDS             complement(3500..3937)
                     /gene="zapA"
                     /locus_tag="IWI39_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9208345.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4139..5056
                     /locus_tag="IWI39_001231"
     CDS             4139..5056
                     /locus_tag="IWI39_001231"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9208346.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(5144..6055)
                     /locus_tag="IWI39_001232"
     CDS             complement(5144..6055)
                     /locus_tag="IWI39_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9208347.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(6456..7034)
                     /locus_tag="IWI39_001233"
     CDS             complement(6456..7034)
                     /locus_tag="IWI39_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9208348.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7054..7722)
                     /locus_tag="IWI39_001234"
     CDS             complement(7054..7722)
                     /locus_tag="IWI39_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208349.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(7737..8807)
                     /locus_tag="IWI39_001235"
     CDS             complement(7737..8807)
                     /locus_tag="IWI39_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208350.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(8978..10342)
                     /locus_tag="IWI39_001236"
     CDS             complement(8978..10342)
                     /locus_tag="IWI39_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9208351.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(10339..11898)
                     /locus_tag="IWI39_001237"
     CDS             complement(10339..11898)
                     /locus_tag="IWI39_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208352.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(11954..14434)
                     /locus_tag="IWI39_001238"
     CDS             complement(11954..14434)
                     /locus_tag="IWI39_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208353.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(14418..14753)
                     /locus_tag="IWI39_001239"
     CDS             complement(14418..14753)
                     /locus_tag="IWI39_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208354.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(14767..15801)
                     /locus_tag="IWI39_001240"
     CDS             complement(14767..15801)
                     /locus_tag="IWI39_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9208355.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(15891..16571)
                     /locus_tag="IWI39_001241"
     CDS             complement(15891..16571)
                     /locus_tag="IWI39_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9208356.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(16855..17958)
                     /locus_tag="IWI39_001242"
     CDS             complement(16855..17958)
                     /locus_tag="IWI39_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9208357.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(18180..18650)
                     /locus_tag="IWI39_001243"
     CDS             complement(18180..18650)
                     /locus_tag="IWI39_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9208358.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            18983..20623
                     /locus_tag="IWI39_001244"
     CDS             18983..20623
                     /locus_tag="IWI39_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9208359.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            20777..22099
                     /locus_tag="IWI39_001245"
     CDS             20777..22099
                     /locus_tag="IWI39_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9208360.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(22161..22592)
                     /locus_tag="IWI39_001246"
     CDS             complement(22161..22592)
                     /locus_tag="IWI39_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9208361.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(22656..23492)
                     /locus_tag="IWI39_001247"
     CDS             complement(22656..23492)
                     /locus_tag="IWI39_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9208362.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         7037 a         5008 c         4247 g         7972 t
ORIGIN      
        1 tttttgctgt aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat
       61 gaacaccaac agctttttct actacttgac catctttttt caacattaat gttggaatgc
      121 tcatgatacc gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc
      181 tgaattcttc ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac
      241 atggaccaca ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat
      301 taaatgtctt atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg
      361 actagtatag catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta
      421 cttaaattta acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct
      481 tttgacgctc cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc
      541 ttttccgtgg acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg
      601 atctacctca cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac
      661 gtgactacta gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg
      721 agcaacaggc gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg
      781 ccactgattc ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac
      841 ttcgtctccc ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt
      901 caaagtttct tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc
      961 tttcacattt ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc
     1021 tgctttttct tccgcctctg acacgacttc atttgctttt ttctttgctt tttccatttc
     1081 tttttcacgt tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg
     1141 tgcctcagaa acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt
     1201 ctctaaatct gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc
     1261 ttcatcaata acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg
     1321 tccaggaacg ccaatcagca aacgatacgt tggacttaat gtatccacat caaactccat
     1381 gctggcatta atcgtatttg cccgattata tccataaact ttcaattcag gataatgcgt
     1441 tgttgccatc acataagcag actttttacc aagatcatct aagattgcga ttgctagagc
     1501 agcaccttct tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc
     1561 attgactttt gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact
     1621 ttgctcaatc gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg
     1681 actttcttca cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa
     1741 agtcttcaac gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata
     1801 gtcttttccg attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct
     1861 tgcttgtttc aagacgacat gattgtcttc actgattccc ggcacgatag cttttacttc
     1921 tttaccaaat cgtgcttttg cattcatcag atccatttta ccaatcacat aagcattgtg
     1981 gatgatttct tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat
     2041 ctcatttcgt tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt
     2101 tggttcaata aataaagtct gtcctgaagc actttgatcg tggactacac cgccaaatac
     2161 accgcggtat tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc
     2221 atcacttaaa tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc
     2281 ttgttcactt cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt
     2341 cacgcgtccg tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac
     2401 taactgatct gcccacatat acaaacgtgc aaattcgatt tcactgtcat aaagatcatc
     2461 aatgaaacga ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac
     2521 tccattcaag tctgctccga tctcaatccg tttcatatgg ggacggatat tttcaagttt
     2581 aggtataggg atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag
     2641 ccagtttgtg atcgtttgtt cctcgctcac tggtaccaat tctgccagtt cttcttttcc
     2701 ttgtgcagtc actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc
     2761 aaggattcgg ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg
     2821 acatacgcta cgcagccttc ttaaataatc tactctgtac aaaaaacgaa atattttcca
     2881 gcactatttt ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag
     2941 aaatggcgtt ctctcaacga taaagcgagc gaaactatgc tcgccgaatt gattttggac
     3001 cacatctaat ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat
     3061 gattagtgac aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt
     3121 caatccatgg acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat
     3181 cagaaaagca gttcctgcat aaaaagcttc atctaggtga aaggcaaata cctgatcaaa
     3241 aaatgctaac tttgatgttg gcgtgacagc tgggtatgga atgtataatt ccaaatgact
     3301 tgctaatggt ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa
     3361 tatgatttgc aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa
     3421 taaaatcagc aaactcagca tgctacgttc ctttctcttc taactttttt taaatttttc
     3481 ttattttgat aaaaggatct taattagaag atttttgttt aatttgtcct tttcgtttct
     3541 cgtttaaaat ctgttgtgct tccacatgat ttttaatggt atgatctgct tgaccatttt
     3601 ctttcaatac ttcccgagct tcgttctcga ttgcttcaat gcgtttcaca cgattttcaa
     3661 gctctgtaaa tttgatcatt tttttcttta attctgctgt ttcttcttcc agctccaaga
     3721 ttctttcttg tttcttcaat tgatccgaaa gcgcattgac agccatcaag attgctgctt
     3781 gttcattatc catttgtggt gataattgtt tcaattctgc taactgttca ttgatcagct
     3841 tcgttacgat atccatatga tgcttggttt cccgtccgat aatagtatat gtttggttgg
     3901 caatcaccgc tttgtagcgt gttttttcgt gcgccatacg aatcatttcc tcctatttga
     3961 tgaaagtttt atttagtgta ccatgaagtt ggttctttca ccgttctcca caaatatttt
     4021 tcacatactt aactattata tgataaactt taaaaaaatg ctatgtctcg caatctctta
     4081 gaaaacgaaa agtcgtgagt tgcggtatag ttttatgtat aaaaacgagg agaattttat
     4141 gagcaacgtc attatcaaag caacaaacga ccaacttagt caaatgaaag catactatcg
     4201 ctcttatttg ttaaataaac agattcctta tactagcttt gctgcaaaaa aagacgggac
     4261 gaccattacc gcttatacat ccggaaaagt gatgtttcaa ggtacgaatg cagcaataga
     4321 agctgataaa tggggggagg cacaggataa acaagccaag ccagcaaaaa cagcctcttc
     4381 tttgccaaaa aatatcagtc aattatccgt tttaggtagt gatgaggtcg gaaatggcag
     4441 ctatttcggt ccgctaacag tatgtgctgc ttatgtgaaa cgtgatcagc ttgcagaatt
     4501 acgtaaactt ggtgttagag attcaaaaga gttaaaagat agccaaatca tccagcttgc
     4561 tgacatctta aaaaaaacca tcccctataa actgcttgtc ttatctccag aaaaatacaa
     4621 tgagatccaa ccaaaataca atgccgttcg gatgaaagtc gctttgcaca atcaagcgat
     4681 ccatttgctg ctaaaaaaaa tcgagccaga aaaaccagaa gcgattttga tcgatcagtt
     4741 tacgccggaa gcaaatttta aaaaatatgc gcgtttagaa aaaaatcaat tacaagaaaa
     4801 actgtacttc gttactaaag gagaacaata tcatttagct gttgctgctg cttctatcat
     4861 cagtcgtgcc agctttttgg aagagttaca caaagcatca gctgaagctg gaatcaccct
     4921 tccatcagga gctggaacga aatctgacca agtagcggca aaactattgg agaaaggcgg
     4981 catgcctatg cttgaaaaat atgccaaact tcactttgca aatacagaaa aagctttgaa
     5041 gctactaaaa aaataaacgg tgttcaaaag cttgcatctg cgataataag cccggccaaa
     5101 cgaaaaatat gaggagctat ttttgtttgg ctgggcttat ttattactga aaataagtgg
     5161 tcaatgcttg tgtcaaactt gcagcaatct gctgctgata actgtctgtt actaattcct
     5221 ttaaatcact atcattattc atataaccca tctcgattaa aatagaaggt tgaagatttt
     5281 ctcgaagaac ttgatagttc ccagtggaga ttcctcttga atccagaggc agatcagtga
     5341 aggagtcagc aattgtctgt gctaatgttt cctcttgtcc atagtaataa tatgtcgtga
     5401 ttccagttgc ttcattcgca tattccgtag agtctgcgtg taaactgata aagacatctg
     5461 cctttttttc ttcactgtag tcgcatatat ctccaagctg tactaattca tctgtagtat
     5521 gggtcaaata aaccgttgct ccagcatcct ctagcgcctt ttttactttt atggctgtac
     5581 ttaatgttat atctttttct tctgtttgtt catcattgct taatgcccca gtatcatttc
     5641 cgccatgccc aggatctaat acaatcactg catcagatag ctcttccggt tttgtttgta
     5701 tattctttgt gactttggtt ccatcttcga gggctaaata tccagtatag ccatcatttg
     5761 tcttgatctg ataagcattt tttgtttcag aaagaaactc cgccagctct ccgccgttta
     5821 tagttgcaag tggggtagtt agcgagtcat ctgtatatag tgtacggtca ttttttccga
     5881 ttttgattaa tgtctcttgt tctgtatcat caaatgattc ctttgtcttt gtaaccgtac
     5941 tgttcgtact gctgaccgtt tgtccaacta tctttttttg accaaaaaca tctatttgaa
     6001 aacagataat aatgataatg attgcaaata caaaagctcc aattattttt ttcattcttg
     6061 actctcacct cgtagatcca gactaagggc caaaacttaa aatcagctta agtaagcaaa
     6121 aaaggggctg taaaagatat aaccgtagaa aataacatta ctgctaaaat gatttttttc
     6181 atttttggca tatcctgaaa tctccttttg actattcgtg gtgattcatc tacccaataa
     6241 atagtttcct tcattctaca aacaattaat ttcttcgtta ttaaattaaa acacttcctg
     6301 gaacaccgac ataataaata aaaatatacc ctgataatca tgagagaata acaaatttgt
     6361 ctttatgaaa aactacctat ataaagaagc aagatataac gatcttcgtc atatcttgct
     6421 tcttcaccta actattttct ccctatataa cagctttacg cttctcctaa tacaccattc
     6481 aaatataaat cgatcaattg cgccacattt agcggttctt ccacaggtct tttcgaccga
     6541 gtgatgtaag gtgctaacac taagaaaaaa tgccttgctg cgatttcttt tgaaatccca
     6601 tttcttagca cagattggtt tgctgaaaag atttcttcaa aaggttcacg ataatttttc
     6661 ttccatagaa agaaaagatg atctcttgtt tcctttgaaa gattgctttc aagatcgtga
     6721 agcatcagaa acaaatcgac cagatgtgtc tcaatcaaaa actgcgccat ttttgtcaag
     6781 gttacttgaa atcccgtttc gcttgtttga acgatttctc tcaaagcttt tccaacatct
     6841 tttaagtggt tttctaaaac tgccgtatat agtttttctt tatcaccgaa ataatgatag
     6901 agattgggct gggtgatccc aactttttgt gcgatttctc ttgttgaagt gccgagatat
     6961 cccttggaca taaacaagtc agtcgcttct ttcaaaattt tttgctgtgt atctttgttg
     7021 tatcgatgga ccatttttcc acctgctttt tggttattct ttttctttca atacaccatc
     7081 ttccatcata aatattctat cgcatttgtc aatcagccgt gtatcgtgag taaccatgat
     7141 cgttgcttta tttttgtctt ttgattcttt tgccaagata tcgactactt ctcgtgcctt
     7201 ttctgaatcc aaactagcag ttggttcatc tgctagaata atggacggat cattgtacaa
     7261 tgcacgagcg attgcgaccc tttgccgttc tccaccagat agttcttctg gatacttgtt
     7321 gatcaactta tccacgccaa gctgctcaaa taattcgttt gcttcttttt gacgatgttc
     7381 atgtttaatc ttatctacta ataataactg cttttttact gtcagaaaag gaaccagatt
     7441 ggaagcttgc aaaataaagc caatttcttt aaaacgtagt ttaacgcgtt cgctttcttt
     7501 ttgattactg aattcttgat cattgataaa tacagagcca ctgctaggcg tctgcaatcc
     7561 gcctgccaaa gtcaaaaaag tacttttacc tgaaccagaa ggaccgatga ttgcaacaaa
     7621 ttccccttta tcgatactca tggttgtttc tttcaatgct ttaactactt ggtcgccatc
     7681 ttggaaactt ttttctactt ctttaaattc tattgctttc atgttcttcc tccttcttat
     7741 ccaattgcct tcaacggatc gatttttacg attgtccgaa cagaaaatag tgcgcctaac
     7801 acagctacta ctaacatcaa caagctaatg acgccaaaaa acagccaatt gctttggaat
     7861 ggaaccgcat caggtagaac aagggatgtt cccactgttc ctaatagacc aagaagaata
     7921 ccaacaaatg ataaaatgaa tgtttgtgcc actacagaac gcgcaataaa tcctcctgtg
     7981 atcccttgtg ctttcattac accaaagata ttaatctttt gcatcgtcag tacataaatg
     8041 aaaatcccga tcacaatagc cgcaataacg atcaaaaatc cgatcataaa tccaaaagtc
     8101 aatacttgcg cattatatcc aggtaattca ttgatgaatt tagaaatttt gatctgttct
     8161 aaatcttcag gtaaattact gattttacct ctgacaacta ttgcgttaat ccgtgcattt
     8221 tcacttgtat cttctttttc gaagcggatt tcttggtaag cattgatggt tgtatagaga
     8281 actggtgaga cattgaattt ggcatgatcc gtaaagccag ttagtttaaa cgtcttgtca
     8341 ctaccagata acttaactgt atctcctatg gctagaccat attcttcttt caaactaata
     8401 tcgcctactg cctcatcatc gttatcaaaa gcttttcctt caacaagatt gggcatgatg
     8461 aattgatttt tatcgatacc gaagaagcta acattgattt ttccttcttc tgtactgtct
     8521 ttactagtca cgactccagg tgtttgggca agatacgcca ctttcccacc ttcgacttct
     8581 tctgctatct tcttagtaat catggacatc cctagatttg aatttgcatc tttcgaaagc
     8641 acaatcgaat ctgcctccca cttgtcgact gctgttcgat tatcttgagc taagccataa
     8701 gctaacccag tcaaaaaaaa taccaggtag gcgatcaaaa acatcacccc tgcaactaac
     8761 gcataacgta actttgaatg cataatttca tttaatgcta aaaacattta actcctcctc
     8821 ctgaaataat cattttatac aacaatttta tcacttgata aatttttggc gaatattatg
     8881 ctcaaaaaat tcttccgcta tttcattata tgaatttttt attctcgatt gattaatatt
     8941 tattatatcc ccacacaata aaagaccgtg atatttctca cagtctttta tctactctat
     9001 tgctatttgc tctcttagct agtactttaa aagctaactc attgaggtag tgaaaacgag
     9061 aggtgtattc agacagaaat ccaaaatcct caactgctag ctgtggaatc gtagttttgt
     9121 gtttcacgat atccgcaagc aggttcaaat ctgataataa gaattcttgg cttatcagct
     9181 gtccgccaat tatttctaat gtatccggat ggtagatcag ctttgcattt actgttttgt
     9241 tttcttcgtc gtattgtgga aatagatcaa attctcctga acacgaaccg gtattttgtt
     9301 ccaagaatgg cgcttcatct tctgtcagcc ctacagttcc taaaaagtag tctataatga
     9361 ctgctgctgt tatcttttgt ctgaggtcgt aagcggttgg ctgaccactc aaagttagcg
     9421 ctactgctct tgccatatgt acagctcgag taactaaagg taaaaagaga tgccgtttat
     9481 ttttgtcaaa actgactggg acgatatctc caatcgcata gatgtccgga tcacttgtct
     9541 gcagatattc attgactatc acagtctcat cctcatacaa agccactttt tcttttaaca
     9601 atgtcgtatt aggacgagtt tgggcagaaa aaatgaccat atctgccttt attgtttgag
     9661 acagaagttg tacggatact actttctctt ctgatttttt ccaatcgatc aagaattcat
     9721 ttggataaaa atttatttgc ttagtttgca gctgattttg aaaactgttg atcatatcct
     9781 tatcgaaata tcttgataaa acattcggca tataatcaac tagatgaata atgtatcctt
     9841 tatttctcaa agcatctgct aattccaatc caatgtaacc agcaccgaag atcactactt
     9901 cttttgcttc ttttaaagcg gcaagtactt gcttagattg cactagattt ttataggtgt
     9961 agacattttc agcttcaatt tgtttataca aggtgacagg agaggaacct gtcgctacga
    10021 ttaatttatc ataggttatg gtctcgtttt ttccttcttt ggatgcgagg atgagctgat
    10081 gtttgtctgg atcaagttca gttactctcc attcggtgat cagttttgcc ccactttctt
    10141 gaagagtccg catgttatgg gcaacttcag ctaaactacg tatctttcct tttaaataaa
    10201 ggttgattcc atttgaaaca tagcctagtc catctttatg atcttcatcg atcagtaaaa
    10261 cttctgcttt cggttgaagt tttcttagat ttagggcagc tgtgatgcct gcatgggaag
    10321 caccaatgat cacgattttc atgttttatt catccttaaa ttcaattttt ctaaataaca
    10381 ttcttttaaa aattggttca aataggggaa atcataatta tattcgttgc tagtgaatac
    10441 aaaaacttta gcggacttct gtgacgggta ctctttcaat tggctagtca aaatgatatc
    10501 atactgcttt tctttctcgt attcgactaa atcatatttg atcgtattca gcaattgttc
    10561 ctggatgatt tctttcacga tatccgccat tagcctttca taaggaagat cataagcaat
    10621 ttgcagacgt aattctaact gcttgatcag cgtccgtaaa atagatgcat agcgagcaaa
    10681 taattcaaag cgggcatttt cctcgtctac ttcagtaaaa tcaacaatag caaatgtttg
    10741 tctgaccaat ctttcacata aaaccaggaa ttgtttatct tctacttcag ttaaccgctg
    10801 ttttaatcct tcttggccaa aaatattcat ccatcctcta aaataggcaa aatgagcgtg
    10861 attttgcatc actgtcaacg caatcttttg cgaaatctct tctggaaaaa cataaaaaga
    10921 gaaatattct tctatcgatt gtttaaacgt tcgattcaat tgagctacta cgtcgtcttt
    10981 gtatttttcg cttatcaaaa actcctggac atgcgagtta tgtgtatcta atgtaaagtt
    11041 actgatcatg aaaagataaa aaaccaacgt ttcattttga ttccaaggaa aggcatattg
    11101 aaagaagtaa cgggtcaaaa atgtatggag gtccaataaa aattgtgtcg tttgaaacaa
    11161 tccaacagga agattgctat aatctggttt ggattttttc gtacagcgca ttcgcttctt
    11221 cataatggaa agccatattt caaatttcaa tcgtccttct tctgtaatag caaaactaaa
    11281 ctctttttgc agcagatcta aaaaagtgtt tcctgataaa tcactgtatt cttttttgtt
    11341 ttcaagatag ggacgtccaa accaaaataa ttggaaataa aaataacgaa tttgactttc
    11401 tggaccggtc agccgcccgc tttttatttt cagatgaaac tcatttaaag aatcatttaa
    11461 ttccttcact ctgcgagaca aagtagccga actgatatta agttccatga caagtttctg
    11521 ccgatcaaat gagccatttt ccaacagata aagaagtatc tgatattttg tagaatagcg
    11581 caagtaacaa tgataataaa catcgactgc tgctatattt ccatgtctga ttaataaaat
    11641 attcccttct tggataacaa tttccaattg tttgatattt tccaagttag cttgtaaata
    11701 aaacagatct tctttcaagg tagcccgatc aactgataag cgggtaagta cgttattaac
    11761 ggaaacctct cctttttcct ctaataaaag ttggaaaagt ttgatttgcc gttgttcatt
    11821 tttttcaagc aaactgaaaa gttccaagtc cactccttca agcatttctg attttaacag
    11881 cagaagtttc cttctcatcc ttgaaaaaga ggatctgtga cagttatcac agatccatac
    11941 aaattctaat cacttagctg gttggcccag ctgtcagttt ccatgtgacc atgccttgat
    12001 acgttgcgcc aggttgagca ttcgtactgc tatttacctc taagagtaaa ccatcggtat
    12061 cgggaaagtc aacgagaacc tctcctttag ctgttccatc actctcataa atcaacgtgt
    12121 tttgaccttg ctgaatcaat tgctttgatc cagaatgatt gaagtataat tttgttccgc
    12181 ttaattcttg accttgatca ttcttaaata aagaagtcaa tgtacctgtc agttgccaag
    12241 gagacgtgct tggaagccga gtatcccgca catcaaaaga aaaatcgcct tttgattcat
    12301 aataacttgt cttcaacggt ttggggagat cgttaccaaa tgtaaaatca tctggtacat
    12361 taactaatgt taattcttgt ttggggacaa gaattgtttt agcagatgtt tggacaactg
    12421 ggttaggaag cttagcacca tctgctaatg taatattgct ggaaagtaca gctgcctttg
    12481 tcagtatgct gacggattgt aaagaagtat ctacagtatt taattgatag ttgtaagtaa
    12541 gatgaatggt atctcctatt ttgctcaata gatctgctgg gacagaaact ccttgttgaa
    12601 tgccggtatc tgcgatagtt gtaccattta attttaagga accattgata taagtagtat
    12661 tttcaggaag tgagatcgtg atttttttgt cggttatcgc atccccagat tttgttaacg
    12721 tgaatgtcac attttcttga atcgtatctc ctgatagagc agacagggga tctcctgtct
    12781 caggttgtct gctaaggttt tttagctgac tatctgcact cacagatgga aaattagcca
    12841 ctgttatttg aggaagagtg gtgattccag cttgttcaga ctgccatttg tacgcaaaag
    12901 acggatacat gttagtaagt acatcgtcca ccaagcgata atcaaaataa tattgccctg
    12961 aagccaatgc aggtagtgta gaagtagctg tatttacacc cccgttattt gattggatcg
    13021 tttggaaaag ttgatctgtc catcctgtcg ctaatttttg ggttcctttc gaataaagcc
    13081 tgtaatggat cgaacccttt tttagttcag ttgctgacca attggccgtg acttggtacg
    13141 atgcagaaga aaggagcttt ccccactgac tatcggctgt taagtttgtt ggtaaaaccg
    13201 ttccgtctag cttgtactgc tccaatacct ttaaagcaac aggagaaagc aatccgcctt
    13261 gcgcatcttt cccactgtac acgatattgg ctgtctgatt caatccactt gtgcttgctg
    13321 gagtatgagt cagtattgct ccttgtgtaa cgttttttcc tgtaaaatag ttgactacgc
    13381 gatcaaagcg actaaatggc aactgatcaa aacgtccttc agtcagcccc atcgtcaatg
    13441 tccgactatt ggtagagcct ggaaggttct ggttatttcc atccacgtat tctgtggtat
    13501 cagcaaatgt attatatttt cctgttgaat cacttggtga gatgtaacta ttccacgtat
    13561 ttatctctgc acttgcagaa aatgcatatg ctcctggatt caagtttccg ccagttaatt
    13621 taggataaaa tctacctgca gctggagcta aaatcgaatt gaacatacga tcttggataa
    13681 tattattcga atcataaaaa tcaacagaaa aaacggcctt tttagacata gaaggttgta
    13741 atttaccctt ggtcaaaatt ttgtttggac tcgtatcata tttatctccg ttttctgtca
    13801 tcttcgcgac attagctgta acatcaaatt gaagcgggat atcgtccacg ttgaattcgc
    13861 ctcgcgtaat aatagcaata atatctgtca atagagtatt tgtatcccac gaccccatcc
    13921 caaaacgcaa ggtatggctt cctgctgata ataaacttcc gctagtaacc ttgatggggg
    13981 caccggcaat atacaagtag gcgttgttcc agtcaattgc ttttaataca tcactgcttg
    14041 atacatgttc cggcaaattg atgatcgcca caaatgcaaa tccttgtgca gtgatattca
    14101 ccaaatcaag tacatttgtt agtttcacat ggctttccca atgaaaacgg accgtgccac
    14161 ctttccaagg atcatttgaa ccatcgttca aataagtagc agtcaattta ggcaattggt
    14221 tagccccagt tagttggaca agcttcccgc cattgactgc actacgtcca ccatttggag
    14281 gtacgatatt ttcatacgtg tatggatcat aagaaatggc aggaggcgtt gctccaacag
    14341 cttctgccga aggtgaaaag ataaatatac ttaaaacaaa aagcgaagta aaaaagaagg
    14401 agcgaaaaaa tgaaaattta ttttgtagca acatgttcat cctccttttc ctttcttctc
    14461 cataacaaca accacgcaag aagccataat tctataccaa tgatcaaata aagaaaacgg
    14521 atcttttgtt cattcgtctc cgggaatacg ccatgttgat tagattcttt ataaatcggt
    14581 agctgtgtct gctgttttga tgcgccattg actgaaccac ttggatgcgg aattggactt
    14641 agatcataaa atgtaatcgt acctgaactt ttggtatctg cttggactgg ctcttgccag
    14701 ctgaagaaca gcaaacttgc aataatccct cccaataaga tcgatgtttt caacgaaatc
    14761 acctcatcat gttgtttctc cttttttctt tttcttgtgg acccacaaaa acagcaaaat
    14821 caaccataac actagaactg cagcaaccaa tcctgcataa agccatggga aagattgttc
    14881 ttgtactgca aaaacggaat tttcatttac tttttgtgct ttttcttttt tgatcgtaaa
    14941 gttttgtgta aatcgccatt cattactgtc gcctgatttt gctatgattt ctgcgcggta
    15001 atcgccggct tttgctttat caccagctaa atcaagattc aagtgaaaat tcgaatttgg
    15061 tgcaactaaa aatgcattag acgtattttc aatataaagc tgattttcat aaaagatctt
    15121 gctcgtaaat atcaaattgc tgataatcct aggctgagga ttttggaaac gaagctgaac
    15181 agttggcttt gcgttgacct cgtccaaatc tcctgataaa agtttcagat ccggttctat
    15241 cgtctcttga ctttcttgta aaacgactgc gagattataa gcaatacgat ttttgatttg
    15301 tgctccttct gtagcatttt ctgtttcagc ttcttcttta ggtgaaacat gaatacccgc
    15361 taatacacgt cccttaaagc tttttgctgg gattttcact tctataggta cagttgtttc
    15421 tgcatgtgct gatacattga tttccttttt cgcaatgagc aaaacgtcac ggacatcaaa
    15481 cggcaatgtc tcatctaacg ttgggttttt ttgactataa tcgatcgttc caccatcact
    15541 cgttaccgcc ggattgacag agacctcaaa tgttcgttct tcatctgaag aattgctgat
    15601 aattacttct agtgtctgat tttgttccgg tgccactaat aaatcaaaat aaccgattgc
    15661 tttatctatt tggttctctg gaaataccgg tttgattcca aagtctcctg ctgaatcctt
    15721 tgcttggctg attgacggaa aaatgaaaaa tagacttaaa atagaaaaaa acgaaacaaa
    15781 cagtcgttta ctttgattca ttgtccacca catcctttca acaaaaactg cggtaaaaaa
    15841 tccttcacct atagtatttt caccgcagtt tgttgaattt atttcatact ctatgctact
    15901 gggccagcca cgaggttcca agtcaaatca gccgtgaatg ttccagctgc tgctttcggc
    15961 actttcagtg tgatatttgc tggatcataa acagaaagcc atgttcccag accttgatct
    16021 tttgctgctg taaatattgg tgtcgctgca cttgctttac tcaacccagt tacagtttga
    16081 ctagttggtc cagtggattc gttatctgac gtacttttaa cagtggatgt tccaaaatct
    16141 aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat ctgaaaccga aactgtcacg
    16201 ttccaccctt gtgcttggcc atctgcacct acacctcgac gatctgatac ttggagataa
    16261 ggtgtatcgt tttttgaagc gtctacttga tacgtttttg ttccgctttc aatctcgtgt
    16321 gtaccaaatg gcaattcggg aactacatcc agtgttaaag gacctgtctg gccagttgct
    16381 ggattttccg gatctgctgg ctctactggt tttgaaggat cagtagggtt aagaggctcc
    16441 acggtatttg ttggttcagt aaaggtaata tctccttttg aagtcgcttg accctcagca
    16501 aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa gtaataatgt cccaatcatg
    16561 ttctttttca taataaatca ctcctttttc ttagtgacct aagtatagta gagacagtat
    16621 gcaaaaaatc ctcatgaatt catccgctta cttgaaatta tgaccctttt tgtataaaag
    16681 agaaaaaaac aataaaatcg tttacatgaa tattcttcct tttttattaa gtatgaaaaa
    16741 atatttcaat tttatttttt cgtttcaaat tattctatta gtgacatcct tgaactataa
    16801 aaataggagt acggcatcat ctgccgcact cctatttttt tcgtcatttg tttcttaaat
    16861 aaaacgattc tcacgcaatt ttattttttt gatatcttct actgtttgtg ttccagctaa
    16921 ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac acttgatgga ctcctgtact
    16981 tcctcctaaa gacaatccat aaataacggg tctgccaata gcaacaagat ctgcacctga
    17041 tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg ctgtcaaaca cgattggtac
    17101 acgtttatct actgcttctg ccacatattg caacgaatca aacgctgctg gaccaccatc
    17161 tagttgacgg ccaccatgat tagataccca gattccgcca gcaccagatt ctaatgaacg
    17221 atagacatct tcttctgact ggacaccttt cacatagact ggcaaatcag aatgagcagc
    17281 aataaattct acatcttttg gactaagctt ctgtttggaa gatttgtaca cagcatccat
    17341 cgtttggcct actcctgatt gataagcctg aacgatcggc attggcagtg ggaaggtaaa
    17401 cccatttctg cggtctgttt cacggtttcc gccaactgtt gcatccgctg tcaatacgat
    17461 tgcttttgct ccattacgct tggccacttc aagaatatcc aaattgatcc catcatcttt
    17521 gctcatataa aattgaaacc attgcggtgc tttctccccg cctgcttctc ttatctcttc
    17581 taatgtacaa gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg ctacaccttt
    17641 tgcagaagct ttctccgcct tcacatgagc taagccatga gcagcaactg gtgccatgat
    17701 gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt gtatcgggta attctacatc
    17761 ttttaataca tgaggaataa tcagctgatg attgaatgcc cgttcatttt cttgataagt
    17821 aaataagtcg ccagcaccac tgctgatgta accataacca ccagctggaa tcacttgtga
    17881 agcggctatt tctaaatctt ccatattgat aaaatccact attccttcat ttgtacctgc
    17941 ttgatacgtt ttttccattt caatgccttc cctctataaa aaattgaatg aataatatga
    18001 tagcgtttac acactttata ttactcttcc gtattgatac aggcaaataa tctgtttaca
    18061 tttcagtcat tccagttgtt actactctga aagaaatgtt tagaacacaa atagataaaa
    18121 aacgatggga ctttgacaaa agtcgtgtca aagtcccttt tacgaaatac tgtggatgcc
    18181 tattgaaata agtgcgggtt gtttctttgt atttcaatcg tttcttgcca ttcttcgttt
    18241 aattttggat taccgttttc ttccttgaac gcataaggtc ccaatccaaa aagctcagtt
    18301 agtatttgat tcacgattcc atatagctcg gtgtgatcgg acacatgatt attgaagatc
    18361 atctcattat caccacgttt gaatctcaca ttcgggctct catgatgaac ctctatgcag
    18421 ttttcccctt tttttaactg acggatttct gaaaacatct cataaatatc ttctccaata
    18481 aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcaactc agagattatc
    18541 gctttgttgg caccaagctt taatgtatag cttgttgctt ctactacgcc gcatgcattg
    18601 caaccacctt ctgatagttc gctttttctg atcaattgaa cttctttcat tttctgttcc
    18661 tcctcttagt tttctactta tgtatttttt atcacctgac gtaagtgtat tttatcgtca
    18721 cgatcacgtg atttattctt cagagattct atttatctat ctaacatatc ctttattctt
    18781 ctacttttct ttctttagca tataagagat acgcatagta atcaagcaag gattgttgat
    18841 ataaaaagaa caaataccgc tattttgatt tcttttgact ctattactca tataaataaa
    18901 atttattata cctgctcttt ttgactatat cctcatctga ctcctatact tcgtttgtat
    18961 taaaacttag gaggaatcat tcatgcttac agggctaaac ttattaaata atccttttct
    19021 aaataaagga acagctttta caaaagaaga acgggcaaaa tacggaatca ctggtatgct
    19081 tccaagtact gtccaaacat tggaacaaca atctgtccaa gcttacggtc aatacttaag
    19141 caaacagacc gatttggaaa aacgtatctt cttaatgaat ctattcaata cgaaccggac
    19201 attgttttat aaattgatgg gcgaacattt agttgaattc atgcctgtcg tttatgatcc
    19261 tgtcgttgcg gattcaatcg aacaatacaa cgaaattttc ttgaaaccac aagatgctgc
    19321 atttctttca atcgacgaac cagaaaatat caaagcaagt ttgaaaaacg cagcagatgg
    19381 acgcgacatt cgtttgatcg ttgtgacaga tgcagaagga atcttaggta tgggtgactg
    19441 gggtgtgaac ggtgttgaca tcgctatcgg taaattgatg gtctacactg ctgcggctgg
    19501 tatcaatcct gcacaagtct tgcctgtatc aatcgacgct ggtactaata acgaagaatt
    19561 attgaataat ccattatact tagggaatcg tcatgctcgt gtagaaggcg aaacttacta
    19621 cgaattcatt gatcaattcg ttcaatcagc tactgaatta ttcccagaat tgttattaca
    19681 ttgggaagat tttggacgag gaaatgctgc aacgatctta gaaaaatacg aagataaaat
    19741 cactactttc aatgatgata tccaaggcac aggaatcgtt gtactagctg gtgtattagg
    19801 tggattgaat atatcaggtg agcaattgaa agatcaaacg atcttgacat ttggtgctgg
    19861 tactgctggt gtaggtatcg caaacatcct tttagatgaa atgatccgtc aaggtgtacc
    19921 tgaagaagaa gcacgccaac atttctatca agtagataaa cagggtttat tgtttgaaga
    19981 tacaaaagga ttgactcctg gtcaaatccc attcgctcgt aaacgctcag aattcactaa
    20041 tgctgatgaa ttgacaaact tagaagcagt tgtaaaagca atccatccaa cgatcatgat
    20101 cggtacttct acccaacctg gagcattcac tgaagaaatc gtgaaagaaa tggcggctca
    20161 taccccacgt ccaatcatca tgcctttgtc taatccaaca aaattggcag aagcaaaagc
    20221 aaaagatttg attgaatgga cggacggaaa agctttagtt ggaacaggta tccctgctgc
    20281 tgatgtggaa tataacggtg taacttacca aatcggtcaa gcaaataatg cgttgatgta
    20341 cccaggttta gggcttggtt tgattgcttc aacagctaca cgagtaaatg ctgaaatcat
    20401 ttctcaagct agtcgagcat taggcggaat cgttgatgta acaaaaccag gagcagctat
    20461 cttgcctcca gtagcaaaaa tcactgaatt ctctcaaacg attgctgaaa ctgtcgcaaa
    20521 atcagttgtt gcacaaaaat tgaatcgtga agaaatcacg gatatcaaag aagctgttga
    20581 atctgctaaa tgggttcctg aatataaatc tttagaagat taagattgtt atagtaaaag
    20641 aacatacaga aactttgtat gacgtctaaa aagaaaaaag cgactgcggc aaaacagcag
    20701 ccagccaact actgctcttt tgccgcttct ctttggcttt tttcatacga tacagaataa
    20761 gaaaggatct gtaaaaatgg cacaaaaaac aacaaccgca actgcagttg aggaaaagaa
    20821 aaaagaaggg ttttggtcaa caaaaattac cggcgtaagt ttgccccttt atttgatcat
    20881 ggtccttgtc ctgatcattg caatggcttt aggaaaatta ccgacgaata tgattggccc
    20941 aatcgctgtt ttggtcattt tcggtaattt atttcatttt atcggaacaa aaatccctat
    21001 cgtcaaaagt tatttaggtg gtggctccgt ttttgcgatc tttgcctctg ccgcgattgc
    21061 tacatttggg atcttacctg agtatgtagt aaaatccaca gaaaactttg tcagcaatat
    21121 gggcttttta gacttttata ttgctgcctt gattactggt agcatccttg gtatgaaccg
    21181 taatctattg atgaaagctt ctgttcgttt tattcccgtt gcacttattt caatggtcgt
    21241 ctcctttttt gccgtaggac tcgtaggtat gcttatcgga aatggttttg caaattccgt
    21301 tttatatatc tcattgcccg caatggctgg tggtgttgga gccggagctg tacctttatc
    21361 aacgatttat gcaaatgttt taggtacgga cgcttcttct atcatttccc gcctgattcc
    21421 tgcgacagca atgacaaatg tcctagctat cattggtgca gcgttagttg ctcgtgcggg
    21481 acaatcaatg ccaaaattca acggtaatgg taaattaatg gaaaaaggcg accttggcga
    21541 tggaaaacct agaaatgtaa aaccttccat tccacaatta ggtgtcggtt tgatggtatc
    21601 attgactttc ttcatcttag gacaaatctg taactatttt gttcctaaag tccatgctta
    21661 cgcattcatg atcattatcg ttgttatctg taaaatcgca aacctattac cagaatatta
    21721 cgaagatgca gctatcatgt tcaacaactt gattgtcaaa aacttgacag cagctgttct
    21781 tgccggtatc ggtattgcgt tattgaatct aaatgtattg gcttctgctt tgacatggca
    21841 atttgttgtc ttatgtttga caagtgttat cgcgatttca gtagtttcag gatttgtcgg
    21901 tcgtttgttt ggtttatatc caatcgaatc aactattact gctggcttat gcaacaactc
    21961 gatgggtggt acaggaaatg tagctgtctt atctgcagct aatcggatgg aacttattgc
    22021 ttttgctcaa atgggcaatc ggcttggtgg tgccatcgtt ttgatcatct caggtttctt
    22081 gatgcaatta ctttcttagt caaaaaaaat acctgatcat caagtatcgg cttgatgacc
    22141 aggtattttt tgataaaaat ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa
    22201 tgataaccga tgcgttcata aaatcggtgt gcttctactc tgtattctgc agaattaagt
    22261 cttattgctg atattcctcg tcttttcgct tcttctacgc cagtcatcaa ggcctttccg
    22321 attcctcttt tttcagcatg tttagacaca gcaagtgcca taatattgaa catcggatcg
    22381 ctgaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga
    22441 tcttcataga ccaagaaaaa atttttttct ggctccttca acagtttctt catttgtctc
    22501 accgtaacgg ttaatggtac atcatatcct aattgttccg cattgatttc tcgtagtgct
    22561 tctgcatctc ctaaacgcat ttcccgcaac atccaaatca ccagccaatt tttatttttt
    22621 taaatgaatt tttttatcac agtatactcc ttttttcatt tagaacaata tgtttcagtg
    22681 attttgactt gcgttagtct agattctcct aatgtttgta agatgacaaa ggcattgtaa
    22741 caattgacca aaagaaccac gaggacaatt acttgaaaaa taaaggtcac tttttttgga
    22801 gacaaaaccc tgctaaatct tgctccccac aaacctccaa taacagcagc ggggataaca
    22861 taaaacaaca tagataaatc aaagcgcata aagctacttg ttaaagcaat cgttaccaat
    22921 tttgccagtt gagaaaagaa aatcgtagat agagaatata gtgttgcttc ttttattggc
    22981 aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc caccaccaat acctaaaaaa
    23041 ctagctaaaa agcccagaac ccccccacat ataaggtacc aagtccatga cgtcaaatga
    23101 aattttggct gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa
    23161 aaaatttgga tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat
    23221 acaatgttgc cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc
    23281 caattcagtg atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat
    23341 accgcaactg ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga
    23401 atcggtttga tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga
    23461 acgatcacca caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc
    23521 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
    23581 aagcttttct actttttaag gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg
    23641 tcctgcatca agtaataaga acagccaaac aaaaatagct cctcataatt ttcgtttggt
    23701 tgaacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
    23761 gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
    23821 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag agactgtgaa
    23881 gtcaacgttt tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc
    23941 cgtttttcga gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga
    24001 ttgtgacaag acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt
    24061 ctattcttct tccgccatga atgagaaacc catatcaaag aactataaag gttatcaaaa
    24121 cagcgtttat gactaaatga attagagtta tctatcacta tttttcaagc gatttaaacc
    24181 aaatggcata caatatggca tacaaaaact tcaataagcc gtgggggttt ttaaatttct
    24241 tcttgttctt taaaatcaat gagt
//
LOCUS       DACDOA010000034        24256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDOA010000034 DACDOA010000000
VERSION     DACDOA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.034"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            137..994
                     /locus_tag="IWI39_001248"
     CDS             137..994
                     /locus_tag="IWI39_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9208363.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="IWI39_001249"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="IWI39_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9208364.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="IWI39_001250"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="IWI39_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9208365.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="IWI39_001251"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="IWI39_001251"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9208366.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="IWI39_001252"
     CDS             3750..4508
                     /locus_tag="IWI39_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9208367.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="IWI39_001253"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="IWI39_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9208368.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="IWI39_001254"
     CDS             5436..5735
                     /locus_tag="IWI39_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208369.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5761..6468
                     /locus_tag="IWI39_001255"
     CDS             5761..6468
                     /locus_tag="IWI39_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9208370.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="IWI39_001256"
     CDS             6465..7232
                     /locus_tag="IWI39_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9208371.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="IWI39_001257"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="IWI39_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9208372.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9200..9517
                     /locus_tag="IWI39_001258"
     CDS             9200..9517
                     /locus_tag="IWI39_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9208373.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9517..9867
                     /locus_tag="IWI39_001259"
     CDS             9517..9867
                     /locus_tag="IWI39_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9208374.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /locus_tag="IWI39_001260"
     CDS             10034..10972
                     /locus_tag="IWI39_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9208375.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /locus_tag="IWI39_001261"
     CDS             10987..11817
                     /locus_tag="IWI39_001261"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9208376.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="IWI39_001262"
     CDS             11861..12886
                     /locus_tag="IWI39_001262"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9208377.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="IWI39_001263"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="IWI39_001263"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9208378.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="IWI39_001264"
     CDS             13964..14404
                     /locus_tag="IWI39_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9208379.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="IWI39_001265"
     CDS             14407..15009
                     /locus_tag="IWI39_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9208380.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="IWI39_001266"
     CDS             complement(15077..16183)
                     /locus_tag="IWI39_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9208381.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="IWI39_001267"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="IWI39_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9208382.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17730..20075)
                     /locus_tag="IWI39_001268"
     CDS             complement(17730..20075)
                     /locus_tag="IWI39_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9208383.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20354..21256
                     /locus_tag="IWI39_001269"
     CDS             20354..21256
                     /locus_tag="IWI39_001269"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9208384.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21469..23244
                     /locus_tag="IWI39_001270"
     CDS             21469..23244
                     /locus_tag="IWI39_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9208385.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23501..>24256
                     /locus_tag="IWI39_001271"
     CDS             23501..>24256
                     /locus_tag="IWI39_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9208386.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7748 a         4334 c         5089 g         7085 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    17701 agtcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    17761 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    17821 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    17881 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    17941 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    18001 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    18061 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    18121 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    18181 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    18241 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    18301 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    18361 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    18421 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    18481 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    18541 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    18601 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    18661 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    18721 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    18781 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    18841 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    18901 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    18961 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    19021 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    19081 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    19141 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    19201 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    19261 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    19321 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    19381 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    19441 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    19501 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    19561 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    19621 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    19681 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    19741 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    19801 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    19861 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    19921 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    19981 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    20041 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    20101 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    20161 atttcttttt taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    20221 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    20281 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    20341 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    20401 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    20461 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    20521 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    20581 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    20641 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    20701 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    20761 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    20821 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    20881 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    20941 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    21001 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    21061 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    21121 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    21181 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    21241 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    21301 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    21361 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    21421 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    21481 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    21541 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    21601 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    21661 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    21721 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    21781 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    21841 agtttctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    21901 acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    21961 atttattacc gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct
    22021 gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact
    22081 gtagtcgcgt taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa
    22141 gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt
    22201 tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa
    22261 aacgagacaa catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt
    22321 ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag
    22381 atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct
    22441 gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta
    22501 aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt
    22561 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta
    22621 gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca
    22681 ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat
    22741 gcctatgcat ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa
    22801 gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa ttggatcgat
    22861 agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc
    22921 atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata
    22981 ttacttatga tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct
    23041 ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg
    23101 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    23161 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg
    23221 aaaaaagctt tatcttccaa ataaaaacat actaagatta gaggagaaca tcattgacga
    23281 atgatgttct cctctatttt ctatttttag attatagtga agagtgaaag aaagttcgaa
    23341 caaagccttg aggactatga gtccctatca aaaaccgaac ttctttactg tcattttaga
    23401 atcaggttta cgtatgttgg ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa
    23461 gcagtaaagt tctttcaaaa cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt
    23521 agatgtttca atgggacaca gctacagtgt cctatacaaa gacgacacct gcttatttga
    23581 agacgagatc gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt
    23641 gcctgaacgt ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa
    23701 gttctttcaa gatcatcact tttcttactg tttgttaaac ccgttagaag cgaaaaaaca
    23761 gatggaagaa aacaccttat gcagttggaa aacagataaa tcggatgccc atcggctcgc
    23821 acagactcat cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga
    23881 gatgcgcgat ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat
    23941 ggatttacac aactgccttc agttgacttt tccagagctt gaacagttct ttttaaacag
    24001 gctgacccct tacgcattga tattgatccg tctttttccc catccagatt ttgtattggc
    24061 atctactcgg acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc
    24121 gaatcgtgcc gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc
    24181 tgttgaaaaa gacagtatcc actgtcaaaa aacgatctat tacgctgaac ttctccaaga
    24241 tttactcgaa caaaaa
//
LOCUS       DACDOA010000035        24246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDOA010000035 DACDOA010000000
VERSION     DACDOA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.035"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(43..1338)
                     /gene="purB"
                     /locus_tag="IWI39_001272"
     CDS             complement(43..1338)
                     /gene="purB"
                     /locus_tag="IWI39_001272"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9208387.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1351..2484)
                     /gene="purK"
                     /locus_tag="IWI39_001273"
     CDS             complement(1351..2484)
                     /gene="purK"
                     /locus_tag="IWI39_001273"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9208388.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2468..2956)
                     /gene="purE"
                     /locus_tag="IWI39_001274"
     CDS             complement(2468..2956)
                     /gene="purE"
                     /locus_tag="IWI39_001274"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9208389.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3372..4679)
                     /locus_tag="IWI39_001275"
     CDS             complement(3372..4679)
                     /locus_tag="IWI39_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9208390.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4682..5263)
                     /locus_tag="IWI39_001276"
     CDS             complement(4682..5263)
                     /locus_tag="IWI39_001276"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9208391.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5379..5476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5835..7127)
                     /locus_tag="IWI39_001277"
     CDS             complement(5835..7127)
                     /locus_tag="IWI39_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9208392.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7124..7747)
                     /locus_tag="IWI39_001278"
     CDS             complement(7124..7747)
                     /locus_tag="IWI39_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208393.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7750..8586)
                     /locus_tag="IWI39_001279"
     CDS             complement(7750..8586)
                     /locus_tag="IWI39_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9208394.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8603..9490)
                     /locus_tag="IWI39_001280"
     CDS             complement(8603..9490)
                     /locus_tag="IWI39_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9208395.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9506..10789)
                     /locus_tag="IWI39_001281"
     CDS             complement(9506..10789)
                     /locus_tag="IWI39_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9208396.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11472..12155)
                     /locus_tag="IWI39_001282"
     CDS             complement(11472..12155)
                     /locus_tag="IWI39_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9208397.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12173..12790)
                     /locus_tag="IWI39_001283"
     CDS             complement(12173..12790)
                     /locus_tag="IWI39_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9208398.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12800..13624)
                     /locus_tag="IWI39_001284"
     CDS             complement(12800..13624)
                     /locus_tag="IWI39_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9208399.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13617..14177)
                     /locus_tag="IWI39_001285"
     CDS             complement(13617..14177)
                     /locus_tag="IWI39_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208400.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14351..15307
                     /locus_tag="IWI39_001286"
     CDS             14351..15307
                     /locus_tag="IWI39_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9208401.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15612..16568)
                     /locus_tag="IWI39_001287"
     CDS             complement(15612..16568)
                     /locus_tag="IWI39_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9208402.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16672..17274
                     /locus_tag="IWI39_001288"
     CDS             16672..17274
                     /locus_tag="IWI39_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9208403.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17362..18861)
                     /locus_tag="IWI39_001289"
     CDS             complement(17362..18861)
                     /locus_tag="IWI39_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9208404.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18877..19401)
                     /locus_tag="IWI39_001290"
     CDS             complement(18877..19401)
                     /locus_tag="IWI39_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208405.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19699..20448
                     /locus_tag="IWI39_001291"
     CDS             19699..20448
                     /locus_tag="IWI39_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9208406.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20439..21224
                     /locus_tag="IWI39_001292"
     CDS             20439..21224
                     /locus_tag="IWI39_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9208407.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21193..21909
                     /locus_tag="IWI39_001293"
     CDS             21193..21909
                     /locus_tag="IWI39_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9208408.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22011..23030)
                     /locus_tag="IWI39_001294"
     CDS             complement(22011..23030)
                     /locus_tag="IWI39_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9208409.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23049..24107)
                     /locus_tag="IWI39_001295"
     CDS             complement(23049..24107)
                     /locus_tag="IWI39_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9208410.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7184 a         5222 c         4223 g         7617 t
ORIGIN      
        1 tttgctattc tattttaatt gaattttgca actcactctt ctttattcca aacctactcg
       61 tttgaatatc acgtcaacgt ttttcaaatg atgatgataa tcaaaggcat cgtctatttc
      121 ttctttagat aataccgatg tgatggttgg atcttcttct aacagcgggc ggaactgcac
      181 ttggttgtcc caagcgtacg cagtttttgg ctgaacaaga tcataagctt cttctcttgt
      241 cattccctta tcgatcaatt tcagcataac gcgctggcta taaatcaaac caaatgttgc
      301 agacatattc cgcttcatgt tttcaggaaa gaccgttaga tttttaacga tattgccaaa
      361 acgatttaac atgtaattca ataaaatcgt ggtatcaggc aagatgattc gttctgctga
      421 agaatgagaa atatctcttt catgccataa tgtgacattt tcataggcag tgaccatgtg
      481 tccgcgcacc actctggcta gaccagccat attttctgaa ccgatcggat tccttttgtg
      541 cggcatagca gaagaacctt tttgaccttt cgcaaaaaat tcttctactt cacgtgtttc
      601 ggatttttgc aatccgcgta tctctgttgc aaaacgttca atacttgtag caataagcgc
      661 catcgctgag aaatattcag catgtagatc ccgagggaga acttgagtag agatttcttg
      721 tggacggatc cccaattgtt tacatacata ctcttcaata aatggcggga tatttgcgaa
      781 cgttcccact gctcctgaga tttttcctgc ttctactcct tttgccgcat gttcaaatcg
      841 ttcgatgttc cgtttcattt ctgaatacca tgtggcaagt ttcaatccaa aagtagttgg
      901 ttcagcatgg acaccgtgtg tccgacccat catcactgta tatttgtgtt cttttgcttt
      961 ttcgccaatg atttccgtaa aacgtttcaa gtcttcccgt aaaatatcat ttgcttgctt
     1021 gatgagatag ccataagcag tatctacgac atccgtactt gttagaccat aatggaccca
     1081 ttttctttct tctcccaatg tttcagaaac cgctcgtgta aacgcaacga catcatggcg
     1141 tgttaatttt tcgatttcca agatccgatc gatatcaaat gatgcatgct cacgaatttt
     1201 tttcacatct tcttgcggga tttcaccaag ttctgcccaa gcttcatctg ctaaaatctc
     1261 gacttcgagc cacgcacggt atttgttctc ttccgtccag atattcccca tttcagggcg
     1321 tgtataacgt tctaacatgt tttttctcct ctaatcccaa atattcgttt gtttgatttc
     1381 ttccagcgtt tgctggatgt cttctgtcaa gatagtgata tgtcccatct tgcgaccttt
     1441 ttttgcttgt ttttttccat agaaatggaa ttgccagtct ggcttattct caatcaagcg
     1501 atagctttcc agcagttgtt ctcccaaaac attgaccatt actgcattgc ttatcaaacg
     1561 tactttttct cctaatggcc aaccacaaat acctcgaata tgggcatcga actgactcat
     1621 agaacaagct tcaatagaat aatgtccaga attgtgagga cgaggagcta attcattcac
     1681 atatagcccg cctgtttttg ttaaaaacat ttctacccct aaaacacctg aaaggctcac
     1741 tgcttctgca atcacacggg caatacgctc agcctcctcg atcacatcct gatcaatcgc
     1801 ggctggagca atcgtttcat gaagaatatt gttgtggtgg atgttttcca cgaccggaaa
     1861 agtcgtgaaa tctccttgac catttcctgc caccatgatc gaaatctctt tttcaaacgg
     1921 gatccatgct tctaaaacac aggttccttc tcttaaaagg tccatggcag gagctagatc
     1981 cgcccgactt ttcaaaacat actgcccctt gccatcatag ccgcctcgag tggttttcaa
     2041 aacacaagga tagccgatcc cgtcaatcgc atcttggata tccgtcggac tgacgatcgt
     2101 tgcataagga gcaatcacga tgttattcgt ttccaaaaaa gatttttcta acaggcggtc
     2161 ttgcgtaatc gcaagcaagt ctgtcccttg aggaataaat gacatgggta aaatcgcatt
     2221 taacgcctct acactgacgt tttcgaattc ataggtaatg acatctgttc ttctggcaag
     2281 ctctttcagc gcaaaggtgt catcatagac actttcaatg tgccaatctg caacttgtgc
     2341 ggctggacaa tcgctcacag gatccagcac acctacttta aagcccattt cttttgcact
     2401 aatcgccatc atacgaccta attgaccgcc accgacgatc ccgatcgttg aaccaggcaa
     2461 caaaggttta accaagttga tcactacttt ctataacagt tttagccaac attgaacgtc
     2521 tttcagccaa tttagcctcc aattctaaat catacatgga aagcatctgt atcgcaagta
     2581 gtccagcatt aattgctccc gcttttccga ttgctgtagt agctacaggt actcctccag
     2641 gcatttggac aatcgaaaga agcgaatcca aaccatttaa tgttcgtgat tggaccggta
     2701 caccgataac cggcaaagtt gtttttgatg cgaccatacc cggcagatgc gcggctcccc
     2761 ctgctcctgc aataatgatt ttgattcctt tttttcgagc gttttgtgca tattcaaaca
     2821 tcaaatctgg ggttcgatga gcagaaacga cctttttttc ataagacaca ccgaattcat
     2881 ccaatatttg acatgcttct ttcattgttt cccaatctga agtactcccc ataatcacag
     2941 agatttttgc tgtcattgtt tttcctccca gtttttctac attcatattt taacaatcac
     3001 tttttttgat gtcaaagaaa tttcgaatgt taacaaagaa aaacatttta ttgttcgtgt
     3061 ttattaaaga taaaaaaaag gaatgtgaca aaagttaggt cacattcctt tttatggata
     3121 aacggtgttc tcctgccaac tcctcggtca caaggaacaa tattcaacaa acatgagaag
     3181 cttttttttg aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttcc
     3241 ttcaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc aaaatacgag
     3301 gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt cacaaccttt
     3361 cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt tttcaatcca
     3421 ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc atttccagtg
     3481 aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt gaaacccagc
     3541 ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa atcaacttcc
     3601 aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat tcctccacca
     3661 agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa tcctaaaaca
     3721 atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga aagttgaacc
     3781 aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc gcctaagata
     3841 acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac atttttccca
     3901 gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac catggaaatg
     3961 atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata aaagggttgt
     4021 ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc catgaaagca
     4081 gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat gaaccctttt
     4141 gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag cagatttgtt
     4201 ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat tgctacagga
     4261 atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg gaatagtttt
     4321 ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc gataatagat
     4381 ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc tactgcttgg
     4441 atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac tgtcaattgc
     4501 aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt catctgttct
     4561 tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc tgcatacatc
     4621 gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc tttttccacg
     4681 attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca atacgcgcaa
     4741 gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg aaagactttt
     4801 cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat tcgatcaatc
     4861 cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg tcttctgaag
     4921 ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa gaataaacag
     4981 aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg aagatcattg
     5041 gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct tcaatggtca
     5101 caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc atttgttcca
     5161 tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg ccctcgccta
     5221 atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt gctcctttat
     5281 catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt ctgttaaaga
     5341 aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct tttacttata
     5401 gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat atataagtag
     5461 gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc acgtgaaggc
     5521 agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc taacactact
     5581 ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat ttttaatgca
     5641 ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct aaaaaagcag
     5701 cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc gaataaatag
     5761 tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg gaagtaattt
     5821 ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac tgcttttcct
     5881 gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc atctacgtga
     5941 gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc atctaataaa
     6001 cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc atatggctca
     6061 ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc ctttttatct
     6121 ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc tttgctgcca
     6181 ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata cgtcaaaatc
     6241 gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat atcatcggaa
     6301 aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata catttctcgc
     6361 aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag ttcgcttcca
     6421 tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat cccataaaag
     6481 tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa attcactaga
     6541 tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt atttcgatgc
     6601 catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa gtggatagaa
     6661 aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg atacggtgca
     6721 taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac cgttattatt
     6781 tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc atatcctttt
     6841 ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat aatgacttca
     6901 gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt ctttactttt
     6961 ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt ttgagataca
     7021 tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc ctcataaaaa
     7081 aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta aagtcattct
     7141 ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt aatgcagtga
     7201 gtaaaaaatc atgctgtata caccagaaca gtccatacaa aaggcataaa taccacagaa
     7261 accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa acaagactat
     7321 ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg gatacaaaaa
     7381 gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc actaataaat
     7441 accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg aaacacatcc
     7501 gtttgatttt tcctttcctc caacatacct ttttttctgc caagtaatgg acagatagat
     7561 aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca gacagcacta
     7621 atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg ataagactgg
     7681 ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct tctattggct
     7741 ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat atttattgaa
     7801 gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa acatactcgc
     7861 gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata ccgtgtaaga
     7921 tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc aataaaactg
     7981 gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt tggtaaagac
     8041 actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg ggatgctgac
     8101 catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg gtaaaatcag
     8161 cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg gaatcaaaat
     8221 cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc tttttccttt
     8281 gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg tccctatcgt
     8341 cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa ttccagcaaa
     8401 ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca aattcagcga
     8461 accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata agaccggata
     8521 aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg ttactgtatt
     8581 attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat ttgaaaatca
     8641 gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta gcatacccca
     8701 tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc gtggcatcat
     8761 tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc gcgattgtct
     8821 gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc ttgtaaaacg
     8881 tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct gggatctctt
     8941 tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac atcaaaacaa
     9001 gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg cctaatcgtt
     9061 ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact agagcgatag
     9121 aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa gtcgttcctt
     9181 tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc acaggtacag
     9241 atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca tcactcagca
     9301 tctttatata attatccaag ccgatccatg agactggatt tcccaaacga taatcggtga
     9361 acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat cctaggatga
     9421 atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt tttagttttt
     9481 cttttttcat gccttcctcc tgctgctatc ctagaatgtt tgacgcaaac tcataaagac
     9541 tctctgctgc ttcttctgct gttacttctt cataaatgac tttttcaact aaaagattgt
     9601 attggtcttg aatttccgga ttcaaagcac tatcaaagac attcacttcg ccgttgtcca
     9661 tatcggaaat catttctaaa taatcaaatg tcgcttgcaa agttgcatca tctgcacgag
     9721 cgagctgttc tcgaatattc gacatgattg gtacaccacg ttctcctttt aaaatttcat
     9781 tggcttcttc actgttttga aagaaatcaa taaacttagc agcttcttct ttatgggctg
     9841 agctttttgc catactcagc atttgcgaag aacgtaaagg gataccagat tctccatctg
     9901 cttttctgcg aggtggatta atcaactgaa tctccatatc ttcaggagcc cctgacaata
     9961 tttctgtcaa ctggttactt gatagaaaca ccattgccgc ttctctatcc ctaaccgcct
    10021 gaaaagacag atccgtcgta cttgcagctc caccacctgg ttctggatgg gcccctttgt
    10081 ctaccagatt tttacgcagc tggaaaaaat ctgttagata gtctggattt tcaaatccca
    10141 agctgttaga tagatcttcc cgattataaa aattcaatcc tttctcaact tgtggtattt
    10201 gaataataca gccagctata aaatcttcga atcttgaaat agcatagatt cctaattctt
    10261 cagtgatttg atagctgatt tgttcaaatt catcccatgt ccaatttgaa gctggttcag
    10321 ggatatttaa ttgttcaaat atatttttat tgtaagcaat cgcaaacgaa ttcaccccgt
    10381 tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc taaataggat tcatctgcat
    10441 tagaaatatc aattatccct tgctccacat attcatctaa tggttcaatc tgattgatgt
    10501 attgaggaaa ttgattgttc agttggaaaa tgtcccagat atcatctgat gctgctagtg
    10561 tacctaattt tgtgtaatat ccttcataat cataaaattc ataatcgatc gtcacatggg
    10621 gatttttttc ttcataaagc tcgattactt ttactgttgc atcgtgtcgg acttgcgaac
    10681 cccaccaagc catgttcagc gtgatttttt tgtcatcttc ttgcccacaa gccgtcaaga
    10741 agaataacat aacacttaaa gctaagatga ttctcattct tttcttcatt ctctgcacct
    10801 ctacttccat tgttattttt caatatactg tatttttata cagaacatat ttattaaacg
    10861 atctttttca aggggaataa aaaccaagac ttgccttatc gttctgccta ggatgaacaa
    10921 ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc tttcatcgct aaaaacactt
    10981 cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa ggatatcaga caactgccca
    11041 atatccttga catagctaaa taagcatgct tcttcgtgtg cgctcttccc taaaaaacag
    11101 aaaaataact agtaattctg tatgaaatta ggtacaatat acacgtcgat gcacaagcat
    11161 tgactatggc aacttgattg gtcagttccc gctgactttt caagccccga caatttagct
    11221 gctactaaat tgtcgggtgc cttttttagt ttttattcgt ttttcattct atagaaagcg
    11281 cttcaataaa tcaagacatt tttttgttag aatgctaata ataacaaaag gttttaactc
    11341 ttcttatgta cattacaggt aaaaaaacaa aaaaatttct cttttttttg aattctgaac
    11401 aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc tcgtcttttg
    11461 attctcacct gttattcatg attctttttc tcagtttccc gatatttttc cattttacga
    11521 ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc tgcttgaatg
    11581 gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag ttcaggatat
    11641 tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat attgataatc
    11701 gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac cgtactgata
    11761 actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt aggaccgttc
    11821 aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga agaacgagca
    11881 ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc ctcaataatg
    11941 cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc attcgcattt
    12001 gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa ttttccaaat
    12061 atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc ttttctcaat
    12121 tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt tgctatctcc
    12181 ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc gcataaccgg
    12241 ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag ttggtggccg
    12301 taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac agttgtgtat
    12361 ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg atagttatcc
    12421 attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc ccataagcct
    12481 tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa tttgattcta
    12541 atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt agttcttggg
    12601 catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt tcttgctgat
    12661 tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag agtatcactg
    12721 ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt ttccactttt
    12781 ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct tctagcgtca
    12841 attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag tgccaaggtt
    12901 catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata aatccatact
    12961 gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt gcatccaata
    13021 cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc agacctgtgt
    13081 ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc gcctcctctt
    13141 ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact tgttgttggg
    13201 atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct tcagcagcct
    13261 tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg ttacttaatg
    13321 atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga ccaaccaaaa
    13381 ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga tcttttgttt
    13441 ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta gcagttgttt
    13501 tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca tcatcgctaa
    13561 aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct aaaaatttat
    13621 tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg aacgtaagtt
    13681 tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc gatctgtttt
    13741 tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc atcatattga
    13801 atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa agggatcagc
    13861 tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt gatttgcttg
    13921 tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg caaaatcttt
    13981 tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat cgcttcagat
    14041 ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat cagcaagtat
    14101 tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat aataagatca
    14161 atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag ttttgatcaa
    14221 aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct ctcctcttta
    14281 ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa ctatgaagga
    14341 gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca tcgcacatga
    14401 gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt ccagaacttc
    14461 agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg gaagctatca
    14521 agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa atagacagca
    14581 tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg aaaaagccat
    14641 cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa agaatttgat
    14701 cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac gctcaataat
    14761 ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca gttacaaaga
    14821 accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc tgttggatat
    14881 cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg aagtagttgc
    14941 ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta tcttgcgtaa
    15001 caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc caaaagtcgg
    15061 tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg ctgatcgtgc
    15121 ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata catctgaagc
    15181 aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc ccaatcgctc
    15241 tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc tatggaaaaa
    15301 tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    15361 caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag gaaagctttt
    15421 gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca caaggagcaa
    15481 tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttgaca
    15541 ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa tattagctga
    15601 attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat ttcaataaaa
    15661 cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc agggcgacta
    15721 gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt taaaatttgt
    15781 ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc atagatatgt
    15841 tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc tttaatcaca
    15901 ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg tctgagccag
    15961 tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt cagtaaatgt
    16021 tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc catttgtctc
    16081 aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg cttcgtacga
    16141 tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat taatcccgct
    16201 tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt gaccaaaacc
    16261 gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat gaacaggaat
    16321 aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa cagtgctgtt
    16381 gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt agagcgcttg
    16441 gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa cagcgataag
    16501 ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt tcgcattcct
    16561 atacgcattt acttccccct tttttatttt aattatactt gaataatgaa aaactagcta
    16621 tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc tatgcaattt
    16681 ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt tttatgtatc
    16741 ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc tgctgttttt
    16801 tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat catcggcaat
    16861 acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt tcacaatgat
    16921 tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt cgtgtcagat
    16981 ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct gattgcgttg
    17041 agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga tacttttata
    17101 gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac agaaaagaag
    17161 gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct aagaacaaat
    17221 ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga ctaattttct
    17281 tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc catttttgat
    17341 tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg cctcttttgg
    17401 cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct ctacgatttc
    17461 cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa gcaaatttgt
    17521 ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc ctgtatcgac
    17581 aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca attgcggtag
    17641 catatcgata accatactca agacagctgc ttcgccatat tccttgaagg cattggcgat
    17701 cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg cttctgcttg
    17761 tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac ggaatcgttc
    17821 tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt ctttagcgta
    17881 ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac gacgtgtgat
    17941 ttctttttct tccagttcga tttgtttttg acgttctacg attttaatct gcatttcctg
    18001 ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt ctgcttttgc
    18061 tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt cttttaatga
    18121 ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt ctgcttgagc
    18181 ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat ctcttttgac
    18241 ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc tgacctcttt
    18301 gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag cgacttcttg
    18361 tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca tagaaccaag
    18421 aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt cttctcttgt
    18481 cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac cgattttgat
    18541 gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt caggggtgga
    18601 gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa ctggcaagac
    18661 gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa tgtgcacatt
    18721 tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg ctgtctggta
    18781 cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg cgatcgcgat
    18841 aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca tgctttcata
    18901 aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg ttccccgctc
    18961 agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac cggtaacact
    19021 tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa cagctacttg
    19081 tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca tcggcatcaa
    19141 tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag caataaataa
    19201 aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc ttgtgaaagt
    19261 taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata catctccaaa
    19321 aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat aaatcgtctc
    19381 aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta tcataccaat
    19441 gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat aggaaggttg
    19501 tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc acaataaatt
    19561 ctttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct ccagaacacg
    19621 atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc catattgatc
    19681 aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc tagcattgct
    19741 aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga cccgacacat
    19801 gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt tatcgttgtc
    19861 atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg tcttcctttc
    19921 tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc tgaaaaagaa
    19981 ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca agagattatt
    20041 ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa agatggacaa
    20101 aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg cgccgttttt
    20161 cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat agcagaacag
    20221 ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc catctcttct
    20281 gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca aacagagcag
    20341 ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt ttgtttaaag
    20401 agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat gggatagaaa
    20461 ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca gtcgcaactt
    20521 tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg aaacaatata
    20581 taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc atttctggtt
    20641 tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta aatcaaaatt
    20701 ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt gccggttttt
    20761 tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta gaaagcgatt
    20821 cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa gtgttaggga
    20881 acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac ggacaggtcc
    20941 ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc tgcgatgacg
    21001 gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt tcgacgaagg
    21061 gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc cacggtggat
    21121 ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa ttaccaaaaa
    21181 aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat ggttgcagaa
    21241 ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag tttcagaaat
    21301 gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct gcttgatgta
    21361 tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca aggaaatgat
    21421 atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa tgtgatccgc
    21481 tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca gctggcgatc
    21541 gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagccga tcagaaattg
    21601 atcgatcaag tattcaacca caccactccc attcacgaaa taccatcatt catcgatttg
    21661 cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg catttattct aaaagaagat
    21721 actatttttt caacaaaaga actggctcaa aaaagctcac tgtcacgtat atccgtcaag
    21781 aaatatttga attttttagt agaacaagat atattgaacg aaagaatcaa atatttggaa
    21841 atcggccggc cgctaacctg ttatgaattg acaaagaatg ggcggaaaaa gctcacagat
    21901 tactgttgag tcatcgaatt atcccataca aaatagtcca agtatacaga agtaacaact
    21961 gtttacttgg actattttta aacattttat tgacgaatat ccatgttttc ttatcttaat
    22021 tgacgggctt taggaagaga tacctcattt ttcgtttttc cagtttctat tatttcacga
    22081 aagttctcga aacttgtcga aatcatattt gataatgcct catctgtatt tgaacctaca
    22141 tgcggggtta caagaactct agggtacatt tctactaatt gctggatagc agggtctgga
    22201 atcgtctccc atggcttgaa ggatcggaaa aaaacctctg tttcatttgc aaaaacatcg
    22261 gttgcaaaac cagctaaatg attgcttttc aaagctctta aaatagcttc attatcttga
    22321 agctctccac gggctgtatt aatcaagatg ctaccttttt tcatttgagc taaaaatcct
    22381 tcattgatca tttgatcgtt ttcacctggg atatgaggta catgaatact gacaatatcg
    22441 cttttggcta ataactcttc aagcgagtgg aacgtcaata cttttttagc agcttctgtc
    22501 tgatgaatat cgtagccgat cactttggct cctagtccct taaacaaaac agcttccgtc
    22561 aaccctatcc tgcctgttcc tatgatccct actgtgcaat ttctgatttc acgactaaac
    22621 atttgctcat ccactcgaaa atctttgaaa gatgttcgga tcgtcgtata cgcagtattt
    22681 ctcaaaagca tcatggcaaa agtcaaagat aactctgcta tcgcatttgg cgaatacgat
    22741 ggtacacggg ctactgtcat accaaaatca gctgccgctt gtagatcgat atgattgact
    22801 cccacagtcc gtgtaaaaac ataacgaatc ccatattcct gcatttttgc aagattggtc
    22861 tcatctgcta tacaatttcc cctcaacaat acagcatcat ggtcctttgc tgacacgata
    22921 ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat aatggtaaat attcaatttt
    22981 tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg cgattctcat actttcttcc
    23041 tccatttctt atgaaaataa acctatgcca tgaatcattt gtaatacaac gatccataca
    23101 ggtgtcagta ccacagcaaa aatagttgac agtaaggatg cattggaggc taaaagtgcc
    23161 tccctatcaa aactgatagc ataggctgct gccactgttg cagctggtgt agccatcatg
    23221 ataacgattg ttgccagtgc ttcaaaattg actggtagaa tattcgttgc cgtcaaaact
    23281 gctagaatca cgatattcaa tgcaggtaca aataccactt tgactagact gtaataccat
    23341 gaagtttttt cagatgcagc ttttttgaag cttacttcac ctaatgttgc gccgattgct
    23401 agccacgcca atggtgaact taaaccagct aaataatcca tcggctgaaa tagccacggt
    23461 gcagtctggt caattcgcaa aaacgctact tggcttatct ctccttctgc acctgtaact
    23521 gacacttgtg gcagataccc ttggaacagc caaatgaaaa ggccggcaaa agtagcgata
    23581 atgattggat tgacgaacat cgctttgata tttttagctt ccatcttcaa gccactcatt
    23641 tttatgtatc cataagaata caaaaagatt cggtagccaa tattgaaaat tgaggaatac
    23701 atcacgccga caggaccata aatcgcactg acgataggcg ttccgaaaaa ggtcgtagaa
    23761 ccaaagatcg tcaggacacg caacgttgtt tgcttatccc ctttatagct taagaaaaaa
    23821 ggttttgaaa ctagaataag aataatataa ataactaatc cccaaatcaa gacattgatt
    23881 ccttgcatca gtaattctgt attgatatct tgcatgaaag ctcgaaatgc cagtgctggc
    23941 aaagccactg ttaagacgac tttcgataga atttttccaa cagattcaga aaaaatcccc
    24001 ttttttctac aaacaaaccc taataatata ataaaaactg ttgaagtgat tgcactgaca
    24061 atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata agttcatctt tctcctccta
    24121 attatgttca tttattcaca ataaaaaaag cttttatttt gctaatacgt attaacattt
    24181 ttaattttat ctttacattg ttacttattg aacaatagga aattaaagaa acttttgaaa
    24241 cttatg
//
LOCUS       DACDOA010000036        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDOA010000036 DACDOA010000000
VERSION     DACDOA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.036"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWI39_001296"
     CDS             complement(53..787)
                     /locus_tag="IWI39_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9208411.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWI39_001297"
     CDS             1114..1524
                     /locus_tag="IWI39_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9208412.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWI39_001298"
     CDS             1517..2218
                     /locus_tag="IWI39_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9208413.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWI39_001299"
     CDS             2218..3804
                     /locus_tag="IWI39_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9208414.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWI39_001300"
     CDS             3823..4044
                     /locus_tag="IWI39_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9208415.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWI39_001301"
     CDS             4041..4802
                     /locus_tag="IWI39_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9208416.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWI39_001302"
     CDS             5182..5691
                     /locus_tag="IWI39_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9208417.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWI39_001303"
     CDS             complement(5766..6305)
                     /locus_tag="IWI39_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9208418.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWI39_001304"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWI39_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9208419.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWI39_001305"
     CDS             7372..8169
                     /locus_tag="IWI39_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9208420.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWI39_001306"
     CDS             8197..8832
                     /locus_tag="IWI39_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9208421.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWI39_001307"
     CDS             8852..9625
                     /locus_tag="IWI39_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9208422.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWI39_001308"
     CDS             10033..10830
                     /locus_tag="IWI39_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9208423.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWI39_001309"
     CDS             10808..11221
                     /locus_tag="IWI39_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208424.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWI39_001310"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWI39_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9208425.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWI39_001311"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWI39_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9208426.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWI39_001312"
     CDS             15998..16558
                     /locus_tag="IWI39_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9208427.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWI39_001313"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWI39_001313"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9208428.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWI39_001314"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWI39_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9208429.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWI39_001315"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWI39_001315"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9208430.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWI39_001316"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWI39_001316"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9208431.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWI39_001317"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWI39_001317"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9208432.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWI39_001318"
     CDS             21905..22483
                     /locus_tag="IWI39_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9208433.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWI39_001319"
     CDS             22535..23017
                     /locus_tag="IWI39_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9208434.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWI39_001320"
     CDS             23134..23508
                     /locus_tag="IWI39_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208435.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5114 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccaa
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACDOA010000037        23653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDOA010000037 DACDOA010000000
VERSION     DACDOA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.037"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..506)
                     /locus_tag="IWI39_001321"
     CDS             complement(<1..506)
                     /locus_tag="IWI39_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9208436.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="IWI39_001322"
     CDS             779..3250
                     /locus_tag="IWI39_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9208437.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="IWI39_001323"
     CDS             complement(3353..5089)
                     /locus_tag="IWI39_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9208438.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="IWI39_001324"
     CDS             complement(5086..5790)
                     /locus_tag="IWI39_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9208439.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="IWI39_001325"
     CDS             5921..6424
                     /locus_tag="IWI39_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9208440.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="IWI39_001326"
     CDS             6384..6722
                     /locus_tag="IWI39_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9208441.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="IWI39_001327"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="IWI39_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9208442.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="IWI39_001328"
     CDS             8273..8851
                     /locus_tag="IWI39_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9208443.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="IWI39_001329"
     CDS             8855..9376
                     /locus_tag="IWI39_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9208444.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="IWI39_001330"
     CDS             complement(9455..10009)
                     /locus_tag="IWI39_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9208445.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="IWI39_001331"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="IWI39_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9208446.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="IWI39_001332"
     CDS             10549..10800
                     /locus_tag="IWI39_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9208447.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="IWI39_001333"
     CDS             10925..11716
                     /locus_tag="IWI39_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208448.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="IWI39_001334"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="IWI39_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9208449.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="IWI39_001335"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="IWI39_001335"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9208450.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="IWI39_001336"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="IWI39_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9208451.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="IWI39_001337"
     CDS             14014..14676
                     /locus_tag="IWI39_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208452.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="IWI39_001338"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="IWI39_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9208453.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="IWI39_001339"
     CDS             18096..18410
                     /locus_tag="IWI39_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9208454.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="IWI39_001340"
     CDS             18423..18797
                     /locus_tag="IWI39_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9208455.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="IWI39_001341"
     CDS             18807..19151
                     /locus_tag="IWI39_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9208456.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="IWI39_001342"
     CDS             19222..20574
                     /locus_tag="IWI39_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9208457.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="IWI39_001343"
     CDS             20634..21776
                     /locus_tag="IWI39_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9208458.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="IWI39_001344"
     CDS             21801..22055
                     /locus_tag="IWI39_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208459.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="IWI39_001345"
     CDS             22311..23495
                     /locus_tag="IWI39_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9208460.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23510..23629
                     /locus_tag="IWI39_001346"
     CDS             23510..23629
                     /locus_tag="IWI39_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9208461.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DACDOA010000038        23465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDOA010000038 DACDOA010000000
VERSION     DACDOA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.038"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..1759
                     /locus_tag="IWI39_001347"
     CDS             98..1759
                     /locus_tag="IWI39_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9208462.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1829..2176)
                     /locus_tag="IWI39_001348"
     CDS             complement(1829..2176)
                     /locus_tag="IWI39_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9208463.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2260..3201
                     /locus_tag="IWI39_001349"
     CDS             2260..3201
                     /locus_tag="IWI39_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208464.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3202..4245
                     /locus_tag="IWI39_001350"
     CDS             3202..4245
                     /locus_tag="IWI39_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208465.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4251..5321
                     /locus_tag="IWI39_001351"
     CDS             4251..5321
                     /locus_tag="IWI39_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208466.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5311..6264
                     /locus_tag="IWI39_001352"
     CDS             5311..6264
                     /locus_tag="IWI39_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9208467.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6276..7286
                     /locus_tag="IWI39_001353"
     CDS             6276..7286
                     /locus_tag="IWI39_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9208468.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7571..8392)
                     /locus_tag="IWI39_001354"
     CDS             complement(7571..8392)
                     /locus_tag="IWI39_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9208469.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8489..8767)
                     /locus_tag="IWI39_001355"
     CDS             complement(8489..8767)
                     /locus_tag="IWI39_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208470.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9035..11044
                     /gene="metG"
                     /locus_tag="IWI39_001356"
     CDS             9035..11044
                     /gene="metG"
                     /locus_tag="IWI39_001356"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9208471.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11523..12110)
                     /locus_tag="IWI39_001357"
     CDS             complement(11523..12110)
                     /locus_tag="IWI39_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208472.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12285..13607
                     /locus_tag="IWI39_001358"
     CDS             12285..13607
                     /locus_tag="IWI39_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9208473.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13762..14538
                     /locus_tag="IWI39_001359"
     CDS             13762..14538
                     /locus_tag="IWI39_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9208474.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14531..15100
                     /gene="rnmV"
                     /locus_tag="IWI39_001360"
     CDS             14531..15100
                     /gene="rnmV"
                     /locus_tag="IWI39_001360"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9208475.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15093..15977
                     /gene="rsmA"
                     /locus_tag="IWI39_001361"
     CDS             15093..15977
                     /gene="rsmA"
                     /locus_tag="IWI39_001361"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9208476.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16428..17057
                     /locus_tag="IWI39_001362"
     CDS             16428..17057
                     /locus_tag="IWI39_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208477.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17181..18290)
                     /gene="ugpC"
                     /locus_tag="IWI39_001363"
     CDS             complement(17181..18290)
                     /gene="ugpC"
                     /locus_tag="IWI39_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9208478.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18499..18921
                     /gene="mgsA"
                     /locus_tag="IWI39_001364"
     CDS             18499..18921
                     /gene="mgsA"
                     /locus_tag="IWI39_001364"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9208479.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19244..19780
                     /locus_tag="IWI39_001365"
     CDS             19244..19780
                     /locus_tag="IWI39_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9208480.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19820..21583)
                     /locus_tag="IWI39_001366"
     CDS             complement(19820..21583)
                     /locus_tag="IWI39_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208481.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21586..23301)
                     /locus_tag="IWI39_001367"
     CDS             complement(21586..23301)
                     /locus_tag="IWI39_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208482.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7561 a         4294 c         4772 g         6838 t
ORIGIN      
        1 aaaaaagtgt tgatttttta ataattattc attaaactct taaacaactt cagaaaatat
       61 taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg
      121 tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag
      181 cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc
      241 tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt
      301 gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg
      361 agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga
      421 agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca
      481 acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa
      541 tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg
      601 tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc
      661 tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc
      721 atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc
      781 aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt
      841 aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt
      901 caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa
      961 cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg
     1021 tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga
     1081 tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat
     1141 cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa
     1201 attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt
     1261 gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt
     1321 aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac
     1381 acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact
     1441 aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg
     1501 taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc
     1561 ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa
     1621 gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt
     1681 gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg
     1741 gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca
     1801 aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag
     1861 cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg
     1921 cagacgagag cgtggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga
     1981 ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa
     2041 tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat
     2101 ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga
     2161 tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg
     2221 aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat
     2281 gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt
     2341 ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa
     2401 acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg
     2461 atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag
     2521 ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca
     2581 attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat
     2641 acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt
     2701 gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca
     2761 aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt
     2821 gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg
     2881 gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga
     2941 aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga
     3001 tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca
     3061 atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg
     3121 atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt
     3181 tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat
     3241 atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt
     3301 actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct
     3361 gccgttattt caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt
     3421 gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt
     3481 ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat
     3541 aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg
     3601 ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt
     3661 attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta
     3721 ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac
     3781 ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct
     3841 gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa
     3901 ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt
     3961 gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt
     4021 attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct
     4081 ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct
     4141 tatctattat ggattccggc agtaacttta tcggttatca tgattggctt taacttactt
     4201 gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa
     4261 ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga
     4321 ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg
     4381 gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata
     4441 tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga
     4501 tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag
     4561 atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt
     4621 caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag
     4681 ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg
     4741 tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag
     4801 cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa
     4861 tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc
     4921 gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct
     4981 acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg
     5041 cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag
     5101 gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac
     5161 cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac
     5221 ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa
     5281 aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga
     5341 ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc
     5401 tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc
     5461 actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac
     5521 ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt
     5581 cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc
     5641 attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa
     5701 gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat
     5761 gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg
     5821 cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg
     5881 gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat
     5941 gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta
     6001 ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt
     6061 ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa
     6121 tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat
     6181 tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag
     6241 gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta
     6301 tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta
     6361 cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt
     6421 tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc
     6481 ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac
     6541 gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca
     6601 accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac
     6661 taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg
     6721 ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag
     6781 aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc
     6841 gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac
     6901 agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg
     6961 caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc
     7021 agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat
     7081 ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata
     7141 aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca
     7201 ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg
     7261 gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta
     7321 gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa
     7381 gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc
     7441 tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa
     7501 acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt
     7561 gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga
     7621 ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg
     7681 aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg
     7741 taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga
     7801 ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc
     7861 cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga
     7921 tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga
     7981 ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat
     8041 ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac
     8101 cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa
     8161 agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa
     8221 ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg
     8281 gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg
     8341 ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac
     8401 ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag
     8461 gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga agtatgagat
     8521 tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca cgaatggcaa
     8581 agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa gctgctgtca
     8641 taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata taaaaatagc
     8701 tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa agatgtttct
     8761 tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac cacgtgtgag
     8821 gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt
     8881 caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt tatccagtct
     8941 ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag caaggaaacg
     9001 aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa ctttttatat
     9061 cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt atactacgat
     9121 tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt tttatctcac
     9181 tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc
     9241 gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat
     9301 cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg ttcaaaaaat
     9361 atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag gctggtactc
     9421 tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca aagatgacga
     9481 aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg taaaagaaga
     9541 atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact atgaagaaca
     9601 tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc
     9661 aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac cagtcaagag
     9721 tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata ttactgcact
     9781 aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag atgttcagat
     9841 ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc tgatggccct
     9901 tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga aagacggaaa
     9961 aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac gttatggttt
    10021 agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac
    10081 gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag ggaacttatt
    10141 gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg actatgcctc
    10201 acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg tcggacgtta
    10261 ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct gggtgctgat
    10321 ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa aagacgaaga
    10381 aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc gtatcgcagc
    10441 gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc aactaggatt
    10501 ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt caggaacaaa
    10561 agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag aagttgcttt
    10621 tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca aatgggatcc
    10681 agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa
    10741 agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt
    10801 gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt caggaatcgc
    10861 tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct
    10921 gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg cagaagcacc
    10981 agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc
    11041 atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga
    11101 gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag
    11161 agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt
    11221 tagaataata ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg
    11281 cagattttaa ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga
    11341 atgcaatgac tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat
    11401 aagttctatc actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag
    11461 ataaaaaaac aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc
    11521 tatcacttga ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca
    11581 gtaagcagtg caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa
    11641 tgttgataga gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca
    11701 gctataaacc aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga
    11761 tagaaataaa aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata
    11821 ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa
    11881 aaggaataca acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg
    11941 atgaacaaag tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat
    12001 aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc
    12061 cggttggata aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc
    12121 tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta
    12181 agagggagtt tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt
    12241 atactgaaaa ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa
    12301 aacgagaaat caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag
    12361 gcgtcttctt caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc
    12421 tagcttggct tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag
    12481 ctacagcaat cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat
    12541 tgcccagctt tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg
    12601 cattatcgat tatctttgcg acacaattga cgaacttatt acaattatct accgattatc
    12661 ttttgttgat tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa
    12721 aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga
    12781 ttgtcatatg gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag
    12841 ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg
    12901 cttatgacgg ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag
    12961 atttgcctaa agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca
    13021 attttgtttt tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt
    13081 ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct
    13141 tgatctcggt atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg
    13201 cgatggcttt agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt
    13261 ttacggttcc ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc
    13321 ttggttcttt cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac
    13381 tgatttttat cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata
    13441 aagtaccact ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag
    13501 gtatgacgat gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag
    13561 gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa
    13621 cagacagaaa aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa
    13681 acaagttctt ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa
    13741 gaatgaaaag gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag
    13801 ttcaatgaag atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg
    13861 gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat
    13921 ccaaatatat acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa
    13981 atcgaaaata aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt
    14041 ggcttggatt atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga
    14101 cagattgcta tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt
    14161 gaagacacgt atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat
    14221 agtttcagtg gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca
    14281 tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta
    14341 ccaatcgaac gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga
    14401 ggaaagcgaa atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg
    14461 aatcttccga cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt
    14521 ctaagcgaac atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga
    14581 tacaagaagg ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat
    14641 caatgaagag atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt
    14701 taccgatcct gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca
    14761 ggcaaaacat gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg
    14821 tgtcgagcat gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga
    14881 catttcccaa acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat
    14941 agctggggca caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta
    15001 cacgaacggc aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact
    15061 cgcccaagca atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac
    15121 cctctagaac aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac
    15181 aaaattttct gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg
    15241 aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca
    15301 agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg
    15361 atacgatgca accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt
    15421 tgtcaacagc tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt
    15481 taccttacta tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg
    15541 atgaactagt cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa
    15601 cgaaagctta tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat
    15661 tcatcgtacc aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac
    15721 tgacaagaag agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga
    15781 caaaagcggc atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg
    15841 gcaaagatga ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc
    15901 caaaacgtcg tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt
    15961 cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat
    16021 caagtaataa gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta
    16081 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta
    16141 actctaagtc acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt
    16201 cttattactt gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa
    16261 ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca
    16321 ctcattttgt atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta
    16381 tactaagtcg acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt
    16441 cagttcgaaa actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg
    16501 tcatgcctaa aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat
    16561 ttattgcgat gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg
    16621 gtttcttttt aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg
    16681 cgttgattgg ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt
    16741 ttacttttgc taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt
    16801 tagcagaaat gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt
    16861 attctcaagg atatttttat acagtattca tcttgatggg gattggtggg cttatccaca
    16921 gtttagttga ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata
    16981 tccccgtatt tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga
    17041 agaaaacgac atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa
    17101 taaatcctct aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca
    17161 cagctctaac agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag
    17221 aagtggcctt tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct
    17281 cttgcatcta cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca
    17341 gctccaagta attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca
    17401 agggctactt gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct
    17461 tcataacctt tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca
    17521 tgtccatctg tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat
    17581 ccaataaagc cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg
    17641 atttgttgga taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct
    17701 gtctgatcgt gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc
    17761 tcagcacgca tggctacacg taatttagca tctaaatttg ataatggctc atccattaag
    17821 aagacttttg catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca
    17881 gataaagcag ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg
    17941 tccacacgtt ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata
    18001 ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca
    18061 cggtctttag gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag
    18121 atatcttcca gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca
    18181 acaaaaacga taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt
    18241 tctgcattgt catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc
    18301 acactttctt ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt
    18361 caatgtcatg ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt
    18421 aataaatatc cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata
    18481 tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg
    18541 atggtgaaat tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc
    18601 gggacgactg ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc
    18661 ggaccgttag gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg
    18721 gtgatttttc ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt
    18781 atccgtttat ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg
    18841 ctactaagag gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac
    18901 aagcatccac tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct
    18961 atatgtagct ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga
    19021 taatataaac aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt
    19081 ttctgtggga aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag
    19141 aggatgggat gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc
    19201 gctgcatgat acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt
    19261 agacgcacgt atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg
    19321 actggtagca atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat
    19381 catgggcatt ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt
    19441 aggaatggcc ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat
    19501 tgaaggagca atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat
    19561 tctggctacg cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc
    19621 gttgatgtac catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac
    19681 agtaatatgg ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta
    19741 caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa aaaacaagca gtcttcccat
    19801 cccagaagac tgcttgtttt tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat
    19861 aaccatcttt ttgaagcagc tcatcatggg tgcccatttc gacaatagct ccctgatcca
    19921 tcactaatat ctggtccgca tttttgattg tggacaggcg atgggcaatc acaaaactgg
    19981 tttttccttc catcattgct aagaaggctt gttggatttt tttctctgtc aatgtatcga
    20041 ctgagctggt agcttcatct aagatcaaca tagggggatt actgatcatc gttcgagcaa
    20101 tcgtcaacag ttggcgttgt ccatctgata tctttaaccc ttgaccgcca atcttagtat
    20161 ctaatttttc cggcaaacgt tctacaaatt catacatata agaggctttt agggcatcat
    20221 tgatttctgc gtcacttgct tccggattcc caaaacgaag attttctcgc aatgtactgt
    20281 caaatagcca agtatcttgt aacaccattc caaaactttt acgtaatgta tcacgttgga
    20341 tagagcggat gtcatggtca tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca
    20401 tcagcaaatt aacaagtgtg gattttccgg cccctgtttt tcctactata gcaatggttt
    20461 gacctggata agcagtaaaa ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa
    20521 aagaaacatg atcaaaagag acttttcctt tgatattttt gggttccaag gcataggcat
    20581 ctggcaaatc aggcttttct tctggctgat cgatcaaatc aaatgctcgt tctagaccag
    20641 aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc gataaatggt ttagaaaact
    20701 gacttgagta gatcgtaaag ctggaaatca caccaacagt gattgacgaa ccactcttca
    20761 atgccaatag gccgccaact aaaccaatcg ataaataagc aaggtgatct acaaaacgag
    20821 aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg tcctttttca tatagatcag
    20881 catttagcag ttcaaattgc tgttgactag cttcttcttg ctgaaaagct ttcacgattt
    20941 tctggttacc gattcgttcg gtcacaaagc ccgagatatc cccaataatc ctttgctgtc
    21001 tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact cacaaggaaa ataattggtg
    21061 tagaacaaag aacgaccaat gtcagaagtg gacttagacg catcataaaa ataaacgaaa
    21121 gaatgatgat tgccactcca gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa
    21181 tattgtccat atcatttgta aatcgactga cgatatttcc gtgggagttt tgatcataat
    21241 aacgaagtgg caatcgattc agatgagcaa atgcatcttt tcttaatttt gcaacggata
    21301 agtaagctaa ccgattgctt aacacttgga tgagccattg actgactacg ttgattaaaa
    21361 cgataccacc gaatagaata agtaaatgga acagtcgatt gaagcggacc tgtccttctc
    21421 caatcatttg atcgatactt tgaccaattt gtaacgtcat cacaactgtt gcaatcccat
    21481 tgatgattcc tagtatcaca gctagccaaa tgtcttttgc ataggcagta aggtaaggaa
    21541 tgaatcggcg gagcgtctgc aaggataatt tttttacttt cataactatt cttcctcctg
    21601 tgatgcgacg atttcctgat attctgctga ttgagcaagt aattcttcat gggtaccctg
    21661 agcagcaatc ttaccttgat ccatcactaa gatatgatgt gcttcttgaa tagagcggac
    21721 acgttgggag ataataatca ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc
    21781 caagtctgtt tgataatcca acgcacttaa agagtcatct aaaatcagta atgctggctt
    21841 ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc
    21901 attttccatg acttttgtat ctaaaccatt tggaagctga cggacaaatt cttctgcctg
    21961 agcgattttc agagcttccc aacattcttt ttctgtggcg tctttcttac cccattgaag
    22021 gttttcacga atagtcccgg taaatagtac agaagtttgg ggaacaagag caatttgctg
    22081 acggagatga tccagctgcc aagaacgaac atccattccg tccattataa ctgtaccgga
    22141 actgacatca tagaaacgcg gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg
    22201 accaatgatt ccaaggactg tatttcgctt cagctgaaat gtaatatttt ggagagaaag
    22261 accagaatca ggagtataac ggaagtcaac atggtcaaaa gcgataattg cttcagaaga
    22321 aaaatcggga gtagtactga cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc
    22381 tttcacccga tttcctgaag cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac
    22441 gatcagtgcc aaaagcattt gattcatata attgatcaaa gcaagaactt gtccttgttg
    22501 caagctacca atatttactt tgatcccgcc aactgtcagc aaacagatga tcccgacatt
    22561 catgatcaag gttgtagcag gtgataatag ggcagagata ttggaaacac gtaaataatt
    22621 gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa
    22681 tgcgcggatg acacgaacac cgctaagatt ctgactaacg tgttcattga gctgatctag
    22741 tttttcttgc actttttggt agagaggaac ggtttttttg atgataaaaa ataaaatgat
    22801 tgagaaaata ggcaataatg ctaagaaaaa cagacctact tcccagtcga tcacaaaagc
    22861 catgaccact gacccgatac ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc
    22921 taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc
    22981 aaaatgattc aactcgttgt gagagaaagt attgattttt ttgattagct gattacgtaa
    23041 ctctgtacca aatccttgtg aagctactga agcatagtat tggcaaatga tagcacaaat
    23101 cagaccaatc acagacatag cgaccatcca taagcccatt tcaatgattt ttcctcggtc
    23161 attcattttt agtccattat cgatcaagga agccatcatt aaaggcagga caagttcgaa
    23221 gacagcttca agaaatttga agaacgggcc caaaatgatt tgcttacgat agtcttttgc
    23281 ataacgtaat aactgaatca tatgattcct cctattattt atttgtccac aattgaaaaa
    23341 acaagtacta ccacattgta gcttaggaaa tttagaatga aaagagaaca agccttttca
    23401 taaatgaaag ttttattatg aagaagcttt agaaattgaa tagactattt tttttaaaaa
    23461 aaaga
//
LOCUS       DACDOA010000039        23302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDOA010000039 DACDOA010000000
VERSION     DACDOA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.039"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..839
                     /locus_tag="IWI39_001368"
     CDS             27..839
                     /locus_tag="IWI39_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9208483.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            836..1636
                     /locus_tag="IWI39_001369"
     CDS             836..1636
                     /locus_tag="IWI39_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9208484.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1797..3065
                     /gene="rseP"
                     /locus_tag="IWI39_001370"
     CDS             1797..3065
                     /gene="rseP"
                     /locus_tag="IWI39_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9208485.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3133..4842
                     /locus_tag="IWI39_001371"
     CDS             3133..4842
                     /locus_tag="IWI39_001371"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9208486.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5054..9412
                     /locus_tag="IWI39_001372"
     CDS             5054..9412
                     /locus_tag="IWI39_001372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9208487.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9557..10030
                     /gene="rimP"
                     /locus_tag="IWI39_001373"
     CDS             9557..10030
                     /gene="rimP"
                     /locus_tag="IWI39_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9208488.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10053..11228
                     /gene="nusA"
                     /locus_tag="IWI39_001374"
     CDS             10053..11228
                     /gene="nusA"
                     /locus_tag="IWI39_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9208489.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11250..11543
                     /locus_tag="IWI39_001375"
     CDS             11250..11543
                     /locus_tag="IWI39_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9208490.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11540..11851
                     /locus_tag="IWI39_001376"
     CDS             11540..11851
                     /locus_tag="IWI39_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9208491.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11864..14170
                     /gene="infB"
                     /locus_tag="IWI39_001377"
     CDS             11864..14170
                     /gene="infB"
                     /locus_tag="IWI39_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9208492.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14197..14544
                     /gene="rbfA"
                     /locus_tag="IWI39_001378"
     CDS             14197..14544
                     /gene="rbfA"
                     /locus_tag="IWI39_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9208493.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14701..14874)
                     /locus_tag="IWI39_001379"
     CDS             complement(14701..14874)
                     /locus_tag="IWI39_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9208494.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15322..17684
                     /locus_tag="IWI39_001380"
                     /pseudo
     CDS             15322..17684
                     /locus_tag="IWI39_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18052..18975
                     /gene="truB"
                     /locus_tag="IWI39_001381"
     CDS             18052..18975
                     /gene="truB"
                     /locus_tag="IWI39_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9208495.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18979..19920
                     /gene="ribF"
                     /locus_tag="IWI39_001382"
     CDS             18979..19920
                     /gene="ribF"
                     /locus_tag="IWI39_001382"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9208496.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20272..21057
                     /locus_tag="IWI39_001383"
     CDS             20272..21057
                     /locus_tag="IWI39_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9208497.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21070..21921
                     /locus_tag="IWI39_001384"
     CDS             21070..21921
                     /locus_tag="IWI39_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9208498.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22045..22497)
                     /locus_tag="IWI39_001385"
     CDS             complement(22045..22497)
                     /locus_tag="IWI39_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208499.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22962..23300)
                     /locus_tag="IWI39_001386"
     CDS             complement(22962..23300)
                     /locus_tag="IWI39_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9208500.1"
                     /translation="MKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPMFTDHGTFIIA
                     FSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWELIEQLISFNIE
                     DKKGHDKFWR"
BASE COUNT         7895 a         4054 c         5059 g         6294 t
ORIGIN      
        1 atcttttaag tataggagga aaaagaatgt tacgtttttt tccgcaaaaa aacaaatata
       61 ttcaagaaaa aagcgaattt caatttaata ccgatggacc aattcctcag cacattgcca
      121 tcatcatgga cggcaatggt cgttgggcgc aaaaccgtcg tttgccacga gttgctggcc
      181 ataaagaagg catggaaaca gtaaagaaag tcacaaaaaa agcatcaagg cttggagtga
      241 aagtgttgac gctttatgcc ttttcgactg aaaactggaa gcgaccaaaa gatgaagtaa
      301 gttttctcat gcaactacct gtcgacttct ttgatacgtt cgtaccagaa ttgattaaag
      361 aaaatgtcaa agttcatgtc atgggttatg aaaacgtatt gccagaacat acgcaagatg
      421 ctgtaagacg tgcaattgag caaacaaaaa acaatactgg aatggtattg aattttgctt
      481 tgaattatgg aagcagagca gagatcgtga ctgctgtcca agaaattgcc gaagaagtag
      541 caaaaggaga aatacacgca gaagaaatcg atgatgaatt gattgctaag catttaatga
      601 caggtttctt gcctaaagag ttacaagatc ctgagctgat gatccgtaca agtggagaag
      661 agcgaattag taattttctg ttatggcaga tcgcatatag tgagctgtat tttacaaaag
      721 cgctttggcc tgattttgat ggtgcgcatc ttgaagaagc aatcgcctct tatcaaaatc
      781 gagaccgtcg attcggcggt gtaaaaaaaa cagaagatga gaaaggggat cagtcatgaa
      841 acaacgtgtg gtcacagcag ccgtggcgct agtcttgttt attccgatta tatggatcgg
      901 aggcatagcg gttgagctta cagcagcaat cttagcagta ataggagttt acgagctgtt
      961 tcggatgaaa ggattgacac tcttaagttt tgaaggagtt ttatccgccg ttggtgcagt
     1021 gttacttgtt ttgccaaaag aaagatggtt tttctttctt ccagagaatg taagtacatt
     1081 tatgttattt tatcttatag ttatgattct tttaggaaca tcagttattt ctaaaaatac
     1141 gtatacgatt gatgaagcag gttttcctgt gcttgtcagc ttgtatgtgg gaataggttt
     1201 ccagaatttt gtcaatgcac gaacagaaag cttggctgtt ttgatatttg gtctatttat
     1261 tgtttgggca actgatatcg gagcatatat ggttggtcga cgttatggtc agcataagtt
     1321 aagtcctgat atttcgccca acaaaacgat tgaaggtgcg ctcggtggta ttggtagtgc
     1381 tgttgtagtc gcatttcttt atttattgct atacccagca aaagaactat ttaatcatgg
     1441 tatgtttgtc atgcttctga tgacgattat tttttcagca gtcggacaat ttggtgattt
     1501 ggtcgaatct gcaatcaaac gtcattatga tgtgaaagac tcgggaaata tcttaccagg
     1561 ccatggtgga atcttagacc gtttcgacag tttgctgttt gtatttccta tcatgcattt
     1621 atttggcatt atttagtaag tatacctgcg tttctttgtg aatttcataa aagacgcagg
     1681 tttttatttt tcattatatt gatactattg gtgtctttgg tatactatgg taaaattata
     1741 gtgttttatc gttgttttat aaaaaacaca aaaaacaagt aaaggagaaa aaagctatga
     1801 aaacgattct gacatttatc atcgtttttg gtatattagt gattgttcat gagtttggtc
     1861 atttcttctt tgcgaaacga tcaggaatcc tcgttcgcga atttgctatc ggtatgggtc
     1921 cgaaaattta tgggcatcaa gcaaaagacg ggacaacata tacgcttcgc ctattaccta
     1981 ttggcggtta tgttcgaatg gcaggcaatg gggatgatga aacagaaatg gcaccaggaa
     2041 tgcctctttc tcttttacta aattctgacg gcatcgtaga gaagataaat ttaagtaaga
     2101 aaatccaatt gacaaatgct atcccgatgg aattgagccg ttatgattta gaagatgagc
     2161 tgactatcac tggttatgtg aatggagacg aaacagaagt agtaacctac cctgttgatc
     2221 acgatgcgac gattattgaa aacgacggaa cagaaatcag aattgcgcca aaagacgtac
     2281 agttccaatc ggctaaattg tggcagcgta tgctgaccaa ctttgctgga cccatgaata
     2341 actttatttt ggcaatcctg ttattcatca ttctggcatt tatgcaaggt ggtgttcaag
     2401 tgacgaacac taatcgcgta ggagagatca tgcctaatgg agctgcggca gaagcaggct
     2461 taaaagaaaa tgatgaggta gtcagtgtgg acggaaaaga aatccattca tggaatgatc
     2521 tgacaacagt cattacgaaa aatccggata aaacattaga ctttaagatc gaacgagaag
     2581 gtcaggtgca atctgtcgac gtgacaccga aatctgtaga gtcaaatggc gaaaaagttg
     2641 gccaacttgg tataaaagcc ccaatgaata caggttttat ggataagatc attggaggga
     2701 cgcgtcaagc tttcagcggt tcattggaaa ttttcaaagc cttaggatca ctatttacag
     2761 gtttcagttt agataaatta ggcggacctg tcatgatgta ccaattgtct tcagaagcag
     2821 caaatcaagg tgtaacaact gttattagtt tgatggcgct tctttcaatg aatctcggaa
     2881 tcgtcaatct gcttccgatt cctgccttag atggcgggaa attagtcttg aatatttttg
     2941 aagggattcg cgggaaacca ttaagtcaag aaaaagaagg aatattgaca ttagctggct
     3001 ttggattttt aatgttgttg atggtgctag ttacatggaa cgatattcaa cgattctttt
     3061 tctagaaaaa gttgacaaat agggtataat aacaaacgat aactattttg aggaaataaa
     3121 ggagtatata gaatgagaca atcaaaaatg ttgattccaa cattacgcga agttccaaat
     3181 gatgcagaag tattaagcca tcaaatcttg ctaagagctg gatatatccg ccaagtatca
     3241 gcgggtattt attcatatct gccattagca aatcgtgtat tggaaaaact gaaacgcatt
     3301 atgcgggaag aatttgaaaa aatcggagcg gtagaaatgc tgatgcctgc catcttgcca
     3361 gcagaacttt gggaagaatc cgggcgttac gaaacatatg gaccaaactt gtatcgtttc
     3421 aaagaccgca atgatcgaaa aatgatcctt ggaccaactc acgaagaaac ttttactgat
     3481 ttgatgcgta atgaaatcaa ttcatataag aaattacctt taaatcttta tcaaatccaa
     3541 cctaaatatc gtgacgaaaa acgacctcgc ttcggtttgc ttcgcggaag agagtttatc
     3601 atgaaagatg cgtattcttt ccatgcaacg gaagaaagtt tggacgagac atataaagat
     3661 tacgaaaaag cctacaccga agtattcaag cgttgtggct tagaatttcg ctcgattatc
     3721 ggagacggtg gcgcaatggg cgggaaagac tcaaaagaat tcatggcgat ctcagaaatc
     3781 ggggaggata cgatctgtta ttcgactgaa agtgattatg ctgcaaactt ggagatggct
     3841 acaagctact atgtatcaaa aaaatcgcat gaaacacagc ttgaattgga gaaagtagct
     3901 acacctaacg taaaatcaat tgacgaagta gcagatttct tttctgttga acctcaaaaa
     3961 atcatcaagt ctgtattgtt cattgcggat gaacaacctg tccttgtttt ggtacgcgga
     4021 gaccatgaag tcaatgacgt aaaactgaaa aactttttat ctgctgactt tttagaagaa
     4081 gcaacagaag aagaagcaca aaaatatcta ggtgctgagt ttggttctgt cggaccagtc
     4141 ggtgtttcag atgacgtaaa agtatatgca gaccgttatg tccaagattt agcgaatgcc
     4201 attactggag cgaacgaaac aggattccac tataaaaatg tgaatccaga acgcgatttc
     4261 actgtacttt cttatgaaga tcttcgtttt gtacaagaag gagatccttc accagacaac
     4321 aatggtgttc ttgcctttac tcgaggaatc gaaatcggac atatcttcaa aatcggcact
     4381 cgttacagtg aatctatggg agcaactgta ctagacgaaa atggacgaga aaaatttgtg
     4441 attatgggct gttatggtat cggtgtaagt cgtttattat cagcaatcgt agaacaacat
     4501 tcagatgaac aaggaatcaa ctggccaaaa ggaattgcgc catttgattt gcatatcgtc
     4561 caaatgaatc taaaagacga ataccaaaca aacctgacga atgaactaga agaagcgatg
     4621 acaaaagctg gctatgaagt actggttgat gacagaaatg aacgcgcagg agtgaaattc
     4681 gcggattctg atttgattgg ctgtccaatc agaatcactg tcggtaaaaa agcagtggat
     4741 ggaatcgtag aagtgaaaat caagaaaact ggcgaaatgg ttgaagtacg taaagaagaa
     4801 ttggtgaata ctttaccaat cctcttgaac caaggcgaat aataagcgtt aaaacacaga
     4861 aaataaaaat agctgaatca gctgtttttg acaatctgat gattcgcttt taaaattgga
     4921 actgcgattt gaatcgcagt tccaattttt taatgtaaaa ctcagaaaaa acgagtatac
     4981 taaaaggtac tgaaaaagaa gaggataaga tgcgatcaat tgcattgttt ctcatcagaa
     5041 agggcgtata tctttgtcag aaaaacgaga tttatttgaa aaactgttgg aacaaatcca
     5101 actagaggaa gcagaaaaaa atcatccact gcttactgct ggtgaaatgg atagtgtagt
     5161 ggtccatcgt aaatcgcgtt tatgggaatt tacgctgcat ttttccaaaa ttttgcctat
     5221 tatgctatat cggtcattga ctcagcatct gactatcgct tttaaagata ttgcacaagt
     5281 caaactgaat atcttagcag atgaccagac attcgatgaa cagctcttgc aagattactg
     5341 ggcccaagct ttggaaaatc agcaatgcga tacaccacta gcacagcaag tattgaaaac
     5401 gcaggtacct gtgataaaag atcgtaaagt catcttgcca atcgacagta caggagctat
     5461 ttcttatttg aagcaacaat acttgccatt agtagaagag ttgttcgtca gttacggatt
     5521 cccaaaattt cgtatcgaac cagaagtgga tgagcagcaa gcggaacgag tattgaaact
     5581 ttttgaagaa cgcaaacaag aacaggcaga agcctttatg aagcaagcag ctgaaagtct
     5641 agttgtccac gaacaaaaga aaaaggaaag aaaagagcaa tcgccagctc ttgaaggacc
     5701 catccacatc cagctaggac gaaatattcc agccgatgaa ccaacgacac caatgatcag
     5761 tatcgttgaa gaagaacgaa gagtcacact tgaggggtac gtattcgaca aagaagtcag
     5821 agaactacgt tcaaaacgca agatcttgac tttgaaaatc acagattata cttcatcatt
     5881 tatcgtcaaa aaattctcga acggcgaaaa agatgaacaa gtatttgatg cgatcagtgt
     5941 cggtagctgg ttaaaggtac gaggaagtat tcaagaagat acgtttgtcc gtgatttggt
     6001 catgaatgcg caagatatca tagaagtcaa acatacacct cgcaaagatt atgctcctga
     6061 aggggaaaaa cgagtagagc ttcatgttca tagcaatatg agtacgatgg atgcaacaaa
     6121 cagcatctct gatcttgtgg cacaagcagg gaaatgggga caccgagcta tcgccattac
     6181 agaccacggt ggtgcgcaag cctttccgga agcacatagt gctggaaaaa aagcaggtgt
     6241 gaaaatctta tacggcgtgg aagcgaatgt cgtagatgat ggtgtcccaa ttgcttataa
     6301 tgatgcacat gaagcattga gtgaagccac atatgtcgta tttgacgtgg aaacgacagg
     6361 attgtctgca gtatatgaca cgatcatcga gctagccgca gtcaaaatgt ataaaggaaa
     6421 tgtgatcgaa agctttgatg aatttatcga tccaggacat cctttgtcac gaacaacggt
     6481 agatctgact ggtatcacgg atggaatggt tcgaggatca aagtcggaag aagaagtatt
     6541 gcggatgttc cttgaatttt caaaagatac aattttggta gcccacaacg cggcttttga
     6601 tatggggttc ttgaatacta gctacgcaag gtatggtatt cctgaagcgg ccaatccagt
     6661 cattgatacg ttggaattag ctagatatct gtatcctcaa ttcaaacgtt tcggattagg
     6721 tgtgttatct aaaaaatttg gtgtcagctt ggaacagcat caccgagcaa tctatgatgc
     6781 tgaagcaact ggtcacttag catggatctt tgtgaaagag gcaatggaca atcataacat
     6841 gctttatcat gatcagctaa atgagcatat cggagaagga gattcttaca aaagggctcg
     6901 acctttccat gtaacgattt tagcaaaaaa tcaggcagga ctgaaagatt tgttcaaact
     6961 gatttcaatg tcgaatgtcg aatacttcga acgcgtccca cgaataccac gctcccaatt
     7021 gaaaaagatg cgtgaaaatc tattgatcgg ctcagcttgt gataagggag aaatattcga
     7081 agccatgatg caaaaagggg tagaagaggc aagaaaccga gcaaaattct acgattatat
     7141 tgaagttatg ccaaaagcgg tctatgcgcc tttgatcgag caagaattgg taaaaaatga
     7201 acatgatttg gaagaaatta tccaaaattt ggtagaaatc ggaaaaagtt tggataaaat
     7261 cgttgttgca acaggtaatg tacattatct gaacgaagaa gatgccattt accgaaaaat
     7321 tttgatcaat tctatgggcg gagcgaatcc gttgaatcgt catagtttgc cggatgtcca
     7381 tttccgaaca accgatgaga tgctaactgc atttcacttt ttaggggaag aaacagcgaa
     7441 agagatcgta gtagaaaata cgaataaaat cgcagatatc tgcgaagaag tgattccagt
     7501 caaagatgaa ctttatacac cgaagattcc aggttcagaa gatgaaatat cagaattgag
     7561 ttacacaaaa gcaaaacaaa tgtacggaga tccgttacct gagatcattc aaaaacgatt
     7621 aaaaaaagag ttgaattcta ttaatggtaa tggcttctcg gttatttatc tgatcgccca
     7681 aaaactagtg cataaaagta atgaagatgg ctatctagta ggttcacgtg gctctgttgg
     7741 ctctagcttt gttgcgacga tgacaggaat cactgaagtt aatccgttag cccctcatta
     7801 ttattgtcca gagtgccaat attccgagtt ttttgaagat ggtacgtatg gttctggatt
     7861 tgacatgcca gaaaaacaat gtccaaaatg tggtgcacgc ttaaacaaag acggtcatga
     7921 tattccattt gagacatttt taggattcca tggagacaaa gtgcccgata ttgatttgaa
     7981 cttctcaggt gattatcaag cagaagccca taactatacg aaagtattgt ttggcgaaga
     8041 ctatgtttac cgtgccggaa cgattggtac agtcgccgac aaaacagcat atggctatgt
     8101 aaaaggatac gaaagggaca ataatctgca gtttcgaagt gcagaagtcg acagattagc
     8161 aaaaggcgct actggagtca aacggacaac cggccagcat ccagggggga tcatcgttat
     8221 tcccgattat atggatgtct atgattttac gccgatccaa tatccagcag acgatcaaaa
     8281 ttcggaatgg aaaacgactc attttgattt tcactcgatt cacgataatg tattgaaatt
     8341 agatattcta gggcatgatg acccaactgt tattcggatg cttcaagatt tatcagggat
     8401 cgatccacaa acgatcccta cggatgaccc ggaagtgatg cggatatttg ccggtcctga
     8461 agtacttgga gtaagtcagg aacaaattta ttccaaaaca gggacattag gtattccaga
     8521 attcggcact cgttttgttc gagggatgct agaagaaacg catcctacaa cttttgcaga
     8581 actactgcaa atttccggac tatctcatgg tacagacgta tggttaggaa atgcggaaga
     8641 attgattcga cgaggagatg cgacactggc ggaagtaatc ggctgtcgtg atgatattat
     8701 ggtttatttg atccatgctg gattagatag cgggatggca tttaaaatca tggaaactgt
     8761 tcgtaaagga caatggaaca agatccctga tgaattgcga gaaacctatc tatcggcgat
     8821 gaaagaaaat aatgtgcccg actggtatat cgattcttgt tcaaaaatca aatatatgtt
     8881 tccgaaagcc catgctgcag cttacgtatt gatggctctt cgtgtcgctt atttcaaggt
     8941 ttatttccct attttgtatt actgtgcata cttttctgta cgtgcagacg actttgatct
     9001 agtctccatg tgcaaaggaa aagatgctgt gaaacaagca atgaaagaaa tcacggacaa
     9061 aggattagat gcttccgtaa aagaaaagaa tcaactgaca gtgctggagt tagccaatga
     9121 aatgctggaa cgaggattca aatttggaat gattgatttg tataaatcgg atgccgttaa
     9181 ctttgttatt gaaggtgata cgttgatcgc accatttaga gctgtcccaa gtttgggaac
     9241 caacgtggca aaacaaatcg ttgaagcaag aaaagacggt ccatttttat ccaaagaaga
     9301 tcttgccacc cgcggtaaag tttcaaaaac attgatcgaa tatatgaacg acaatggcgt
     9361 attgaaagat ctaccagatg aaaatcaatt atcgttattc gatatgctat aagcatctgt
     9421 tttcctgtct gcttgtaatt ttaagcagag tatggtatag ttcttatagg taatgatact
     9481 aactgtgagt gagcggattt ttcgctcact ctttttaatg gaattacgca cttaattttt
     9541 taaggaggcg aaacttttga gtagcgtcgt tgaaacagtc acagaaatgg tgacaccgat
     9601 tttagatgaa caaaattttg aacttgtcga agtcgaattt gtaaaagaag gaaaaagctg
     9661 gttcttacga gtatttattg acaaagaagg cggtatcgat atcgaagaat gcgcctttgt
     9721 cagtgagaaa ctgagtgaaa aacttgatgc agcagatcca gatccgattc ctcaagctta
     9781 tttcctggag gtatcctccc cgggagcaga gcggccattg aaaaaagaaa gcgactatga
     9841 aaaggcactt ggtgattatg tccatgtctc cttataccaa ccagtagatg gcgaaaaaca
     9901 atatgaagga tttcttcaat catttgatgc tgaacagctt acattgaaaa tacgcataaa
     9961 aacacgagaa aaagaattga cttttgaccg aaaaaatatt gcaaaagctc gcttagctat
    10021 ccaattttaa tcacggagag gaatcagaaa aaatgagtaa agaaatgtta aatgcgttag
    10081 acgctttaga agctgaaaaa gggatctcaa aagaaatcgt gatcgatgct ttagaagcag
    10141 cattggtttc tgcatacaaa cgtcactatg gccaagcaca aaacgtcgaa gtggaatttg
    10201 atcagaaaaa aggaaagatc catgtctatg cggtaaaaga agtaacggaa gaagtgatgg
    10261 attcacagtt ggaagtctca ttgaaagatg cacttttaat caatccagca tatgagatcg
    10321 gtgatacgat ccgttttgaa gtaacaccaa aagattttgg gcggatcgct gcgcaaacgg
    10381 ctaaacaagt tattttacag cgtgtacgtg aagcagaaag aacgattatt tataacgaat
    10441 tcagtgctta tgaaaaagac atcatgcaag gaatcgtcga acgccaggat aaacgttata
    10501 tttatgtcaa tcttggtaaa attgaggcag ttctttctaa acaagatcaa atgccaaatg
    10561 aattttatca accacatgat cgaatcaaag tttatgtttc ccgagtagaa aatacgtcta
    10621 aaggtccgca agtatttgtt agccgcagcc atccagacct attgcgccgc ttatttgagc
    10681 aagaagtacc agaagtctat gatggaatcg ttgaaattgt cagcatcgcc cgtgaagctg
    10741 gcgatcgttc aaaagtggca gttcgctcaa atgatccaaa tatcgatgca gtcggtacat
    10801 gtgtcgggcc aaagggacaa cgagttcaag caatcgtcaa tgaattaaaa ggcgaaaaca
    10861 tggatatcgt cgaatggaat gaagatccag ctgtattcat tgcaaatgca ttgaatcctg
    10921 cacaagtcat ggatgtgatc ttcgatgaga caaatccaaa ggcctgcaca gttgtcgttc
    10981 cagactacca attatcgcta gcaatcggta aacgcggaca aaacgcacgt ttagctgcta
    11041 aattgactaa ccataaaatc gatatcaaat cagaatctga catgacggaa ttttatgaaa
    11101 atcaagaaca acaaaaagaa acagaagaac tgcatgatga agcaatcgtt cagtcagatt
    11161 taacagacga tgaatatgaa acaattgcat tcaacaatga aacggtcgaa gaaaaaccag
    11221 aagcttaact ttagtaagga ggcaaagaga tgaaacaaag gaaaattcct ttacgcaaat
    11281 ccgtggtttc aggtgaaatg aaacctaaaa aagagatggt ccgaatcacc cgttcaaaag
    11341 aaggggaagt atcgatcgat ccaactggaa aaatgcctgg acgaggagcg tatgtctcac
    11401 ttgagccgga agaagtacaa caagcttggg ataaaaaaat tctcgatcga gtactcgaaa
    11461 caacattaac agatgagttt tatcaagaac ttcttgacta tgtcactcac caaaaagcac
    11521 gtaaagagtt atttggaaaa tgaatacagt taatcggcaa aaagccatga atctcatcgg
    11581 tctagcgatg cgcgcgggaa aactaatcac gggtgaagaa ctgaccattg ctgaaatccg
    11641 caaacaaaaa gcaaaattgg tgtttgtggc aaccgatgca agcgagaata caagaaaaaa
    11701 gataaaagat aagagttcat attatgaagt tccttgcttt gagctatttt ctgaagcaga
    11761 aatcacgcag atgatcggaa aacctcgcaa ggtattcgga ataatggatg ccggatttgc
    11821 gaagaagacc aaggagctaa ttgaaggtta ggaaggtgat tgcatgggca acaaaagaat
    11881 ctatgaatta gcaaaagaac tcaaaaaatc tagtaaagac gtcgtggaaa aagcacaaca
    11941 acttggcatc gatgtgaaaa accatatggg tgcgattact agcaatgatg agaagaaatt
    12001 gcaacaagcg ttcaaaaacc caagcacgaa acaacaagct gcacaacaag caagcagaaa
    12061 accagccaat caacagccga acgatcaacg taaaaaagaa acaacgaaca atcaacaaaa
    12121 aaataaaaat aaccgcagtt atcaagatcg cggtcaaggg agcggtcaag tgaatcaagg
    12181 aaaaaaacca acaacaaacc aaaataatac gggaaatacc agcagccaaa atcgtcaagg
    12241 aacgagccaa ggcggcaatc aaaaccgtac taacaataac aacaaaccaa acaataacaa
    12301 tcgcggcaaa tttaataata ataaccgtaa tcgttataat aaaaaaggta aaaaaggcaa
    12361 acagcaacaa tcaactaaac cagctgtacc accacgcaaa ttccgtgaat tgccagatgt
    12421 attagagtat actgaaggta tgaacgtagc agatattgcg aaaaaaattc atcgtgagcc
    12481 agcagaaatc attaaaaaat tatttatgct tggtgtcatg gtcaatcaaa accaagcact
    12541 ggataaagat acgattgaac tgttagcgac ggactatggc atggaaccac aagaaaaagt
    12601 ccaagttgac attgctgata tcgataaatt cttcgaggca gaagaagtcg atccagataa
    12661 attagtctca cgtccgccag tcgtaacgat tatgggacac gtagaccatg ggaaaacaac
    12721 tttattggat acattacgcc attcacgtgt aacaagcggc gaagccggcg gaatcacaca
    12781 gcatatcggt gcctaccaga tcgatattga tggtaaacca atcactttct tggatacacc
    12841 aggacatgcg gcctttacaa gtatgcgtgc acgtggagca agtatcacag atatcacgat
    12901 cttagttgtt gctgcagatg atggcgtcat gccacaaaca gttgaagcaa tcaaccatgc
    12961 caaagctgca ggcgttccaa tcatcgtcgc tgtaaataaa attgataaac caggtgcaaa
    13021 ccctcaacat gttatgcaag aattaagtga gtatgagtta attcctgaag catggggtgg
    13081 agaaacgatc tttgttgaga tctcagcgaa attcggccaa aacatcgaag aattgctgga
    13141 aatgatcctg ttagtcgcag aagtagaaga tttaaaagca gatccaacac aacgcgcaat
    13201 cggaacagtg atcgaagcac gtcttgataa aggtaaaggt cctgtgtcaa ccttacttgt
    13261 ccaagaagga acacttcatg ttggtgaccc aatcgttgtc ggtaatactt acggacgtgt
    13321 ccgtgtaatg acaaacgatc ttggccgtcg cgataaagaa gcaggacctg caacgccagt
    13381 agaaatcact ggcttgaatg atgtacctca agcaggtgac cgtttcgtag tcttcgaaga
    13441 cgaaaagaca gcacgtgcag cgggtgaaga acgcgcgaaa cgtgcattat tagaaaaccg
    13501 tgctgccaca agccgtgtga ctttggataa cttatttgaa agcctgaaag aaggcgaatt
    13561 gaaagaagtt aacgtaatca tcaaagcaga tgtacaaggt tcagcagaag cactggctgc
    13621 atcattgaag aagattgatg tggaaggtgt ccgtgtaaaa atcgtccatt cagctgtcgg
    13681 agcaatcaat gaaagtgatg taaccttagc agcagccagc aacgctatta tcatcggttt
    13741 caacgttcgt cctacaccgc aggcaaaagt gcaagctgat actgaagaag tcgatattcg
    13801 tcttcaccgt atcatctata aagccatcga agaaatcgaa actgcgatga aagggatgct
    13861 agaccctgaa tttgaagaaa aaatcaccgg tcaaatgatc gttcgtgaaa ccttcaaagt
    13921 ttctaaagtt ggtacgattg caggtgccta tgtaacagaa ggatatattc gtagagatag
    13981 cggtgtccgt gtaatccgtg acggaatcgt aatctatgaa ggccaacttg caagtctgaa
    14041 acgattcaaa gatgacgtga aagaagtcaa aatgggttat gaatgtggtg caatgattga
    14101 aaaattcaat gacatcaaag tagatgacgt aattgaagga tttatcatgg aagaagtcaa
    14161 aaatgactaa aaactgataa aaggaggcca atcattatgg ctaactaccg tgaccgccga
    14221 gtcggtcaag aaatcttaaa agaagtcaat gatattttgc gcaaaaaggt ccgtgaccct
    14281 cgtgtggaga acgtaacgat cacagatgtg aaagttacgg gggacttaca gcaagcaacg
    14341 atctattaca gcattttatc tgacttagca tcagataaga aaacagcaca agatggattg
    14401 aataaagcat caggattgat tcgtcgagag ttagggcaca atctaagtat ttataagaca
    14461 cctgaattgt tctttgaact tgatgaatca gttgtctatg gcaaccatat cgatgaattg
    14521 cttcgcgact tgaacaaaga ctaatcaaaa actccctcaa atcctgtgaa atcaggggtt
    14581 gcgggatttt tttgttttca ttaagacgta ttacctaaga gttccatgac tttagtcttg
    14641 cggcgtgtca gctaaccaaa aaagagaaaa agaaaagtaa aaaattcgac tttctttttt
    14701 ctagtagtat ataaattttt gcatatagat aaagatcaag ataccgataa cggaagctag
    14761 attgtaatct attttagtga cagtcaggta tagccaagta actagaagat cagtggaacc
    14821 tgcaccataa aagtttgagt ttgtcggtcc gttgacagtt aaaagaaaaa tcacgttgaa
    14881 gttattgata tttccgatta cgccgcttcg atttggtcac gatccaaatt ttggataatc
    14941 cctgtagaaa ttagcatagt agctgggata ccaattcaca tattgacaaa gataacagag
    15001 acttttacat aaaacggatc aatcaggaaa ggaattgctc gatccagccc cagttttcca
    15061 gcaatgtgtt aactggccca gcgtcattca gcaaattatg catgatgagg agcgaaatga
    15121 actgcggaac agccattgtg ataacgacaa gttttttgga tcaatgtaaa aaacgtcagc
    15181 gaaaggaaaa atttcgtggt tttttatacc tgttcatttg gctagatata aaatccaaac
    15241 aaatgaacag aagtgcttat taggtttcaa ttgggagtat ctgcgctaga ataagagtaa
    15301 taatgaaaag gagtgttatg tatggattat tctagtaaag aatactttga taaaatgacg
    15361 gcttggtggc gtgctgctaa ttatctttct gttgggcaac tttatcttaa agataacccc
    15421 ttactgagac gtacactgaa acccgaagat gtcaaaaaac atccaatcgg tcattgggga
    15481 acgattcctg gccaaaattt tatttatgtc catttgaacc gagtcatcaa taaatatgat
    15541 ttgaacatgt tttatattga agggccagga cacggcggac aagtcatggt atcgaatgct
    15601 tatttagatg gaagttacac agagatctat ccagaagtaa cggaagatga gacaggaatg
    15661 caaaaattat tcaaacgatt ttcatttcct ggaggcattg cttctcatgc agcaccagaa
    15721 acaccaggtt ctatccatga aggcggagag cttggatact ctctttcaca tgcagtcgga
    15781 gctgtattag acaatcctga agtaatctct gctgtcgtga ttggtgatgg tgaagcagaa
    15841 accggtcctt tagctggaag ctggttctca aatgtcttta tcaatccagt gatagatgga
    15901 gctgttttac cgatccttca tttaaatggt gcaaaaatag ccaacccaac gatcttggca
    15961 cgtaaatcag atggagaatt agcaaattac tttaacggac ttggctggga acctttcttt
    16021 attgaaggaa atgattcaga aaaattaaat cctgttatgg ctgaaaaaat ggatcaagca
    16081 atcgagaaaa ttaaatccat ccaaaaagaa gctcgcctga agacagcaac agatgtcgtg
    16141 atgccaaaat ggccagtatt gatcgtacgc acaccaaaag gatggaccgg tccaaaagaa
    16201 tgggacgggg aaccaattga aggaaccttc cgagcacacc aagtaccgat tcctgtagat
    16261 caagaacata tggatcatgc agatgcttta ctccgttggc tgaaatccta tgaaccagaa
    16321 aaattatttg atgcacaagg acgtatttta gaagaaatca gagaaatcgc gcctactggt
    16381 gaccaaagaa tggcaaaaaa tcctatcacc aatggtggaa tcgatccaag accgcttatt
    16441 atgccagatt ggaaaaaata tactttgcaa tttgaaaaac caggctccgt taaagcagaa
    16501 gatatgaccg aacttggtaa attcgttcgc gagatcattg agaaaaatcc agaaaatttc
    16561 cgtatatttg gaccagatga aacaaaatca aaccgattga atcaagtttt caaaacgaca
    16621 aatcgccaat ggatggaaaa aatcgaacca gaaaatgatg aatggctttc gccatctggt
    16681 cgcgtcatcg acagtcaatt atcggaacac caagatgaag ggttcttaga agggtacgtt
    16741 ctgactggac ggcatggttt tttgcaagtt acgaatcatt cttgcgagtg gttgactcta
    16801 tgttgactca gcacttcaaa tggatgcgga aatcacatga cctttcttgg cgaaatgatt
    16861 atccttcgct aaatctcatt gcaagctcaa cagtcttcca gcaagatcac aatggatata
    16921 gccaccaaga tccaggaata ttgacacatt tggcagaaaa gaaggcggag ttcatccgtg
    16981 aatatctgcc tgcggatgca aatacattgc tcgcagtcat ggataaagca ttccgctcgt
    17041 cagaaaaaat caatttgatc atctcttcca aacatccgcg tgctcaattt tactcagcag
    17101 aagaagcagc cgtcttagtg aatgaaggct tgaaaatcat tgattgggct tcgacagcaa
    17161 aagaggagga acctgaattg gtcattgctg ctgcaggaac ggaatctaac ttagaagcat
    17221 tagcagctgt aactttgctg ctggaagaat tcccaaaact aaaaattcgg ttcatcaatg
    17281 tcgtagattt attgaaatta cgtcatccaa gtcaagatcc gcgtgggttg agcgatgaag
    17341 aatttgatca atattttaca aaagacaaac cgatcttatt tgctttccac ggttatgaaa
    17401 cattggtccg cacgattttc tttgatcgtc ataaccatca tttaatgatt catggctaca
    17461 aagaaaatgg agatatcacg acaccatttg acatgcgtgt agtaaatgaa cttgatcgtt
    17521 atcatttggc aaaagacgca gctttaaaaa tcaaaggaag tcaagcagaa gattttgcga
    17581 aaaaaatgga tcagaaatta caagaacatc aaaactatat tcgtgaaaat ggtattgatc
    17641 tgccagaagt attggattgg aaatggaaaa atcttgacca ataaaatgat aaaatcgatc
    17701 ccgcaaaata gaatttttta cattgattaa gaaactgaaa aaataaataa aaaaaacgga
    17761 aaaaagactg gaagaatggc cagtcttttt tttcgttttt gggaagtgaa aaccaaacta
    17821 tttacctgta agccacccat aaatccaatc aaatggtgca aagaaaaaat cttccattct
    17881 attccctcct ttaaaaaagc ataacatgat ttctatttac gtgagcatga attttgttac
    17941 gtttttattg ccgaaaaata aaaaccgttc attatgatgt caaattcgtc agtaattgat
    18001 gcaatgtgtt ataataattc agttaaatca aaaatgaaat ggagaaaaga catggacggg
    18061 ttattacctt tatggaaaga acgcggaatg acaagtcatg attgcgtatt caaattaaga
    18121 aaaatcttac atacgaaaaa aatcggacat gggggtacgc ttgacccaga cgtagacggt
    18181 gtgttgccca tttgtattgg aaaagccaca aaagttatcg aatatttgac agattctggg
    18241 aaaatctatg taggcgagat aactctaggt ttcagtacaa cgacggaaga tcgatccgga
    18301 gaacgggtag aaacaaaacc agtagatatg tctttatctg aaaaaaaaat agatgaagtg
    18361 atggcttcat ttattggtga gatcacccag ataccaccaa tgtactcagc ggtaaaagtc
    18421 aacggtaagc ggttatatga atatgcccgc aataatgaaa cagtggagcg tcctgttaga
    18481 aaggctcaga tttaccgttt tatccggaca agtgaattga caagagacga agtgacagga
    18541 accatttctt ggcgcttcga agtagaatgc gggaaaggaa cctatgtccg cacattagct
    18601 gtagatacag gcaagaaact tggttaccct gcgcacatgt ctgatctgac ccgaacagca
    18661 agtgccggta tgtcccagaa ggatgctctg acattagaag aagtagctca agctgtgaaa
    18721 aatgggacaa tcgatgattg tttgctgcca atagaaaaag gagtggaacg gttcaaacga
    18781 gtagatatta ctgatgaagt atggcagaaa gtaaaaaacg gtatgagact tgattatact
    18841 gtatttgcgc tcccagaaat gccgtcagaa gaaattgctt tgttttataa aggaaaagtt
    18901 gtcagtattt atcagccaaa cccgaaagaa aaaaacaaat taaaacctag taaagtattg
    18961 agaaatgagg aataacgaat gaatatcatc aagatcagac atccttacca atcagaacaa
    19021 attccagaag acgatgtcgt tttagtgctg ggatttttcg acggcgtgca tatcggacac
    19081 cagaaagtca taaagacagg tatggaaatt gcacaaaaag aaggattgaa attagcattg
    19141 atgactttca atcagcatcc atctattgtc tttaaaaaga tcgatcctag cagcgtgaaa
    19201 tatttgacta gccttaagca aaaagaagaa aaaatggccg ctttgggtat agattattta
    19261 tatgaagttg attttacttc ttcttttgct catttagcac cgcaggaatt tgttgatcaa
    19321 tacatcgtag ggttacatgc aaaatatgct gtttccggtt ttgattatac gtatggacca
    19381 aaagacattg cagatgtcgc tcattttcct gaatatgcca aaggaagatt caaaatcgtc
    19441 acagtttcta aagaagaaga acaaggtgaa aaaatcagct ctacccgtat tcgtggatta
    19501 ctagacagcg gacaagtaaa agaagcagca aaactattag gttgcacata tgaggtagaa
    19561 ggggtagttg tccatggcga tgcacgaggc agactactag gattccctac agcaaatgtc
    19621 aaagtaaaga gcacggttca tttgcctaaa gaaggtgttt atgtgtcgga aataaaaatt
    19681 ggggaaacat ggtatccagc catgggttca attggacata atgatacatt cggtgaagga
    19741 cgggagctga ctgtagagtt gtatattttg gactttaatc aagatatcta tggtgaacat
    19801 gtcgcggtac gctggaatca atttttaaga gatcaagtga aattttctag tgtagaggaa
    19861 ttgattgaac aactcaagga ggatgaacaa gcaacagctg aatatttttc tcagcaataa
    19921 aacattatgc ataaaatctc ttgtttatgt ataactagca attacaataa aatgggtgat
    19981 aaataagccc aggaaagggg ctgtgacaaa agtcttgtca cagccttttt ttccaaataa
    20041 atggtttttc agaagtagct tcttcggaaa taagccgaaa tttcttgaag ctgaccactt
    20101 ctgtcacaac ctctttcttt atagaaataa aaggacacta gcaattcatg aaaacgattg
    20161 ctagagcgta tcatttgctt tagactatat atcttggtgt acactcactt taaggcggaa
    20221 ataaaagtta tccgtaataa caggtttagg tatgaaaaag gagtgggtac tatgtacaag
    20281 gaattagaaa acaaagtcgc agtagtaact ggtggctcaa aggggattgg aacagcgatt
    20341 gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca actataactc ggataaagaa
    20401 ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg gaaaagcagt ctctgtacaa
    20461 gcagatgtca gttctgaaga aggaatacag cgtttacttg atacggcttt agaaaatttt
    20521 gggacactgg acgtttgggt taataatgct gggatggaaa atcagcatcc aacccatgaa
    20581 cttagccttg aagattggga aagagtgtta aaagtcaatt tgactggcgt ttttatcgga
    20641 acgaaagcgg ctttaaatta ctttacagag aataataaaa aaggaaatat catcaatatg
    20701 tcttctgttc atgaacagat cccatggcca acgtttgctc attacgctgc ttcaaaaggc
    20761 ggggtaaaat tatttaccga aacggtagca atggagtatg caccgaaggg aatcagaatc
    20821 aatagtatag gccctggtgc tatccgtaca cctattaatg cggcaaaatt tgatgatcct
    20881 gaacaaaaag aactcttgga aagtatgatc ccactaggac gcgtaggtga accaaaagaa
    20941 atagcggcga cagctgcatg gttggcttca gacgaatcca gttatgttac aggtattaca
    21001 ctatttgcag atggtgggat gactttatat ccttctttcc aaggtggaaa aggataaggg
    21061 tgaataaaaa tgggtttatt gattgcattg atcccggcag ttggctgggg gatccagcca
    21121 ctgattttga aaaagattgg tggcagacca acaaatgaga tattaggaac tggtattgga
    21181 gcggtgcttg tcggcttact cgttcaatta ttcgcttctc cagctggtat ttcagtgact
    21241 acatttttga ttagtttact ttctggtgca ttttgggtta ttggacaaac aggacagtat
    21301 acatctttca acttgattgg tgtttctaaa acgatgccaa tttcgactgc attacagtta
    21361 gtcggaacct cgttgattgg tgtcttcgct tttggagaat ggtctggaag tatggggaaa
    21421 atcattggag cagcagcaat cattcttctt gtatttggtt ctgctttaac ggcaatcagt
    21481 gatgatcctg gaaaaaaagg cggtatcaca aaaggaatta gtctattagc atcaacaagt
    21541 attggttatt gggtatacag tgcattgcct aaactagttg atgcaagcgg actttccatt
    21601 ttctttccgc aaatgatcgg tgtcttttta ggggcagtga tttatgtgat tttccgtaaa
    21661 ccaaccgcat tttctgaaga caagagttgg aaagcaacga tcgttggagc catctttagt
    21721 gtgtcagcat tggcttatat tttttcagca aatgaaaatg gggttgctac tgcatatatc
    21781 attacacagc taaacgtagt tatctctaca ttaggcggta tgtacatcct gcatgaaaat
    21841 aagacaccaa aagaaaaaaa attaacgatt atcggattat tgctgatcgt agtaggaagc
    21901 atggtcacgg tctttatttg aggataaaga tggtaaaatt tcatttatta aaaaaatatt
    21961 tgtttgaacc aaacagtaag tacttataaa aaaatagcgc tagaatttcc aacatctctg
    22021 gaatttctag cgctttttaa ttgtttaaga tacaagggaa atatctgtct ggatcaataa
    22081 tttttcattg atccattgac taaagtttac gacataattg aagttaccac cgattagtat
    22141 ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga cggtcttttt tatttgcgta
    22201 aacatcgtta tggtactgga ctaattgttc tcggaattga ttgattgctg taaaatcacg
    22261 atattgaagg ttgaaacgca tcgtgattgg tgcatttagc atgaatcgct gcatttcttt
    22321 tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa agattacgtt cagaaaattt
    22381 ccattgcccg ccaacctttg ttccttttag ttttccttca tgaagataat ttctgataga
    22441 acgtgtagta acacctaatt gttcggctaa ttcatttact gagtaaaaat ctttcaatgt
    22501 tcaccctcct atgtgaatgg ttctactgac ttagttgata tttgcttaat cacgaaatgt
    22561 agaaagaacg attttaatta aggtcattgt aaaaatcaag tcagaaaatt ctaactttta
    22621 tttctctaaa gaaattcgtt ctctcctaaa gtgatattat atttaaatat acctgaaaat
    22681 aatgggcagt attatgaaag atcactgata ttttctcgta ttttttcctt attcaaaagt
    22741 tttgcaggaa aattgctgaa aaaataattt tactaagtag aaaattcaaa gccattcaat
    22801 cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac tttgtcaatt
    22861 aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta taaggtgatg
    22921 aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca aaatttatca
    22981 tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc ccagttgatt
    23041 acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc ttcttggata
    23101 tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg atttttagca
    23161 aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt ccattttaca
    23221 gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt
    23281 tgatgttctt tttctttgat tt
//
LOCUS       DACDOA010000040        22669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDOA010000040 DACDOA010000000
VERSION     DACDOA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.040"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2131)
                     /locus_tag="IWI39_001387"
     CDS             complement(<1..2131)
                     /locus_tag="IWI39_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208501.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
     gene            complement(2128..2832)
                     /locus_tag="IWI39_001388"
     CDS             complement(2128..2832)
                     /locus_tag="IWI39_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9208502.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2844..5153)
                     /locus_tag="IWI39_001389"
     CDS             complement(2844..5153)
                     /locus_tag="IWI39_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9208503.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5348..5812)
                     /locus_tag="IWI39_001390"
     CDS             complement(5348..5812)
                     /locus_tag="IWI39_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208504.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5812..6438)
                     /locus_tag="IWI39_001391"
     CDS             complement(5812..6438)
                     /locus_tag="IWI39_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9208505.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6445..6810)
                     /locus_tag="IWI39_001392"
     CDS             complement(6445..6810)
                     /locus_tag="IWI39_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9208506.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6800..7138)
                     /locus_tag="IWI39_001393"
     CDS             complement(6800..7138)
                     /locus_tag="IWI39_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208507.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7128..7454)
                     /locus_tag="IWI39_001394"
     CDS             complement(7128..7454)
                     /locus_tag="IWI39_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9208508.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7435..7713)
                     /locus_tag="IWI39_001395"
     CDS             complement(7435..7713)
                     /locus_tag="IWI39_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9208509.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7715..9079)
                     /locus_tag="IWI39_001396"
     CDS             complement(7715..9079)
                     /locus_tag="IWI39_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9208510.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9092..9667)
                     /locus_tag="IWI39_001397"
     CDS             complement(9092..9667)
                     /locus_tag="IWI39_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP9208511.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9633..10820)
                     /locus_tag="IWI39_001398"
     CDS             complement(9633..10820)
                     /locus_tag="IWI39_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9208512.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10884..12611)
                     /locus_tag="IWI39_001399"
     CDS             complement(10884..12611)
                     /locus_tag="IWI39_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9208513.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12608..13060)
                     /locus_tag="IWI39_001400"
     CDS             complement(12608..13060)
                     /locus_tag="IWI39_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP9208514.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13172..13552)
                     /locus_tag="IWI39_001401"
     CDS             complement(13172..13552)
                     /locus_tag="IWI39_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9208515.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13549..13935)
                     /locus_tag="IWI39_001402"
     CDS             complement(13549..13935)
                     /locus_tag="IWI39_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208516.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13937..14215)
                     /locus_tag="IWI39_001403"
     CDS             complement(13937..14215)
                     /locus_tag="IWI39_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208517.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            14263..14991
                     /locus_tag="IWI39_001404"
     CDS             14263..14991
                     /locus_tag="IWI39_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP9208518.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15137..15613)
                     /locus_tag="IWI39_001405"
     CDS             complement(15137..15613)
                     /locus_tag="IWI39_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP9208519.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15573..15920)
                     /locus_tag="IWI39_001406"
     CDS             complement(15573..15920)
                     /locus_tag="IWI39_001406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208520.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15907..16092)
                     /locus_tag="IWI39_001407"
     CDS             complement(15907..16092)
                     /locus_tag="IWI39_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208521.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16093..16365)
                     /locus_tag="IWI39_001408"
     CDS             complement(16093..16365)
                     /locus_tag="IWI39_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP9208522.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16362..16565)
                     /locus_tag="IWI39_001409"
     CDS             complement(16362..16565)
                     /locus_tag="IWI39_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208523.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16690..16902
                     /locus_tag="IWI39_001410"
     CDS             16690..16902
                     /locus_tag="IWI39_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208524.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16935..17129)
                     /locus_tag="IWI39_001411"
     CDS             complement(16935..17129)
                     /locus_tag="IWI39_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208525.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(17142..17324)
                     /locus_tag="IWI39_001412"
     CDS             complement(17142..17324)
                     /locus_tag="IWI39_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208526.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17951..18316)
                     /locus_tag="IWI39_001413"
     CDS             complement(17951..18316)
                     /locus_tag="IWI39_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208527.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18356..18658)
                     /locus_tag="IWI39_001414"
     CDS             complement(18356..18658)
                     /locus_tag="IWI39_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208528.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18658..18957)
                     /locus_tag="IWI39_001415"
     CDS             complement(18658..18957)
                     /locus_tag="IWI39_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208529.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(19120..19389)
                     /locus_tag="IWI39_001416"
     CDS             complement(19120..19389)
                     /locus_tag="IWI39_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208530.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19401..20150)
                     /locus_tag="IWI39_001417"
     CDS             complement(19401..20150)
                     /locus_tag="IWI39_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208531.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(20147..20839)
                     /locus_tag="IWI39_001418"
     CDS             complement(20147..20839)
                     /locus_tag="IWI39_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208532.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20845..21516)
                     /locus_tag="IWI39_001419"
     CDS             complement(20845..21516)
                     /locus_tag="IWI39_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9208533.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21509..21850)
                     /locus_tag="IWI39_001420"
     CDS             complement(21509..21850)
                     /locus_tag="IWI39_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208534.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(22028..>22669)
                     /locus_tag="IWI39_001421"
     CDS             complement(22028..>22669)
                     /locus_tag="IWI39_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9208535.1"
                     /translation="AESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNRRV
                     FFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLEEM
                     KDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFWSE
                     FKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
BASE COUNT         6268 a         4799 c         3523 g         8079 t
ORIGIN      
        1 ccatccattg tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg
       61 ttattggttc tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc
      121 atgttgctgg accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg
      181 gtccacctat ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc
      241 ccaatattcc acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc
      301 tccaagctct cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc
      361 ctccgccaga tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt
      421 ttccctgaat atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc
      481 ctttttctgc agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca
      541 aagaattttc ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat
      601 ctgttgttcc gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg
      661 ttagctccat gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta
      721 acacatcacc ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt
      781 tgatgaagtc agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta
      841 ctccgttgtc tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg
      901 ccacatttgg agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga
      961 catgcccacc agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg
     1021 tttctgttcg attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag
     1081 tgtacagtga taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat
     1141 aagtgtattc gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag
     1201 cgacatcacc aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt
     1261 gttccaacgc caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt
     1321 caaataaggt tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac
     1381 gtttggcaat acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga
     1441 taggttcttt gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat
     1501 tgacaattga ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa
     1561 tccgaaaacc attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat
     1621 caagttccgc gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg
     1681 cttcaactgg cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat
     1741 agttaaattt ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat
     1801 tgccgttatc cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc
     1861 gagctgttac agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc
     1921 atgatccatc tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact
     1981 gtccgcttct tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt
     2041 ctggcaaatc ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt
     2101 ttttataagc ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac
     2161 ttctttaaat tttccggata tttttacctg attccattca ggctgcaata ctggccaatc
     2221 accacgcgta aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac
     2281 gattgttgca gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat
     2341 atctcctccg ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa
     2401 aatatccata acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg
     2461 ctgacaacga aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac
     2521 ttctttatcc atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa
     2581 ttggctttta ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat
     2641 cccttctatt tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct
     2701 tcggccctga atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc
     2761 ctcaatgatg caattcatat caattactgc attccgattt ttccaaataa aatttggttc
     2821 atctggattc ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt
     2881 ttgtttgctt tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg
     2941 actttgccaa tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct
     3001 ttgcgttttt cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt
     3061 aactcaggtc cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct
     3121 aaataaggca gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt
     3181 atctttatat tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata
     3241 ggagctttcc ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct
     3301 tgccatgctt ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg
     3361 aaaattcgtt ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat
     3421 ccaccaaaat aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg
     3481 aagaaagaat tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca
     3541 gtaactaatg cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat
     3601 aaaccagcaa gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta
     3661 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     3721 gcaattgtta tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat
     3781 tcctgtaata aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca
     3841 cctacgtcag ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg
     3901 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
     3961 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
     4021 gtcccgaata cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc
     4081 ttgtcttgta gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact
     4141 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
     4201 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
     4261 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
     4321 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
     4381 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
     4441 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
     4501 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
     4561 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
     4621 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
     4681 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
     4741 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
     4801 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
     4861 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
     4921 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta
     4981 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
     5041 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg
     5101 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
     5161 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
     5221 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt
     5281 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
     5341 ggagttccta tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa
     5401 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
     5461 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
     5521 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
     5581 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
     5641 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
     5701 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag
     5761 cagaattttt taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc
     5821 acctccaact ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa
     5881 tttattaaat aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac
     5941 attattgtat ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc
     6001 ttcctctgca gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca
     6061 aactgctttt ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa
     6121 ttctcgtgcg gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc
     6181 atccatcact tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg
     6241 cgacacacga cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt
     6301 gacaccgatt tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc
     6361 aacagaatct ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa
     6421 agtatcttgt tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat
     6481 aatttcttca attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg
     6541 ttgatttgtt gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac
     6601 tttcacttga tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa
     6661 gagcgactta ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa
     6721 agattccaac gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat
     6781 ttcagaaaaa ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt
     6841 tggttacggt atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc
     6901 cagcattttg cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa
     6961 aagagacttg gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt
     7021 tactcacatt tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga
     7081 acactgctaa ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa
     7141 gctaaactca actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac
     7201 gttacagaat caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact
     7261 ttaattcgtc tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc
     7321 acaccgagtt cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg
     7381 tttccgtctg ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc
     7441 tgtctcttcg gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc
     7501 tttgtcgagt tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat
     7561 aagatattct tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata
     7621 aaaagagagc aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc
     7681 ctctaaagac aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt
     7741 cgtagcgata tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt
     7801 aaatttggaa tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca
     7861 aggcaccaat aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg
     7921 tgtctggttc acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt
     7981 ggtaaccaat tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg
     8041 ttttcatttt ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca
     8101 tcacttctgt cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag
     8161 cacctgaagc agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac
     8221 caggattttc atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat
     8281 aagctttttt caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata
     8341 attttttagt tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa
     8401 actgaatagc tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg
     8461 ctttcttcac aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt
     8521 tacgaagtaa attttcttct tgagcataag taataacttc agatgcaatt acttctggga
     8581 cagtaactga accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat
     8641 ttccagctac aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca
     8701 tttttgcacg tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt
     8761 gagtaatcat tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat
     8821 cgttgtcttc accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat
     8881 tgtcgtctgt ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt
     8941 catcaataac actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc
     9001 ctgattcaac tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt
     9061 tcaataattc tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt
     9121 tgattaattt ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag
     9181 cttctgtatc ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat
     9241 ggattgttcg aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac
     9301 caaaactaca ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg
     9361 ttgtgtccgg taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg
     9421 ttccgctttt agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat
     9481 ccgaattact aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc
     9541 ataaaagtgt ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt
     9601 cttcatcgga acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca
     9661 tttccatcac ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt
     9721 ttctggccaa tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc
     9781 tgtttaggaa aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg
     9841 atgttgtagc caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc
     9901 cattccactc tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg
     9961 tttttcaata ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct
    10021 gtcttcatca gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt
    10081 ttgtaaacac ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg
    10141 atggaatatc cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt
    10201 ccttccagtt gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg
    10261 atgtgggcgt ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc
    10321 aaaacttttt cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca
    10381 gcacctttta atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt
    10441 ttttgaccat tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg
    10501 tacgccaaat gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa
    10561 gtatttgttt ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa
    10621 atatttagta agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg
    10681 gccaatgcca cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg
    10741 atagatgaat ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg
    10801 atatatccac gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc
    10861 aatttttttc accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca
    10921 tctgaatcaa tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg
    10981 aacccatcaa tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta
    11041 gtaattgcta caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt
    11101 ttctcataaa acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga
    11161 gcaacttcaa cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc
    11221 gctggatcat aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc
    11281 aaaatctgat cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc
    11341 aaatcactgt aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata
    11401 aaataaagtt gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt
    11461 aaagatacac aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg
    11521 ccacttaaat caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca
    11581 aagaaattat ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg
    11641 gaatgagctg accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat
    11701 attccccagt taggattgca catttcccag acttttctat ccgtccaatc ataattttta
    11761 tttggctcat agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt
    11821 gcttcgcgat atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta
    11881 aacatcaatg gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt
    11941 ttctcctgtt tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta
    12001 tcaatattct tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag
    12061 tcataagtaa tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt
    12121 aggattggat tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca
    12181 aaatcagatg cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat
    12241 cctaatgaac cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc
    12301 tcacgagtaa cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa
    12361 gaataaataa atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt
    12421 gtgtgaacaa taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac
    12481 caaatatctt ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta
    12541 ggatacttct taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca
    12601 ggttcaatca tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt
    12661 catctactgg ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc
    12721 cagcaattag tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca
    12781 tcttcttatc tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag
    12841 ctcctgattt attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat
    12901 atttgtgttg gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga
    12961 tggtgtacaa atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt
    13021 tttcatcaat aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt
    13081 gttttttgaa aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg
    13141 cgaaaaaatt ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt
    13201 tgtcttccgt ttcgttttca acaattggat gacattttga acataatagc atgagattgt
    13261 ttggatcaag cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc
    13321 tctttccaaa tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa
    13381 attgacgcat atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa
    13441 cagactttgc ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt
    13501 agataccctt tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc
    13561 acgaataaga tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg
    13621 tttcttagca tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc
    13681 agtggactga tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct
    13741 atcaagaatt tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc
    13801 atcactcaaa attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac
    13861 aactactgtc cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat
    13921 tttgatcatt gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca
    13981 ttcgctcata ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa
    14041 taactaacgt attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat
    14101 taccattcca attttctctg tacggactat tctctgcttc aattgctttc ttataaactt
    14161 tagccaaact tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt
    14221 tactctccta tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg
    14281 cttcaagaag ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca
    14341 aatagttgtc cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca
    14401 tatagtgcgt tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag
    14461 tgcaacaaat tttatagttt acaatttata aatacgttta atgaagatga aggatattct
    14521 gaaaatattt taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat
    14581 attgaaaaag tttctcctac atttgttgat atttatactc aagccacaca agctaaatct
    14641 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
    14701 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
    14761 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
    14821 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
    14881 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
    14941 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
    15001 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
    15061 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
    15121 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
    15181 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
    15241 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
    15301 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
    15361 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
    15421 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
    15481 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
    15541 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
    15601 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
    15661 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
    15721 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
    15781 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
    15841 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
    15901 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
    15961 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
    16021 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
    16081 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
    16141 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
    16201 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
    16261 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
    16321 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
    16381 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
    16441 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
    16501 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
    16561 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
    16621 cttttttatt tgatataata cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg
    16681 tgatttatca tgaatatcaa caatttagta aaactcgttg aacaacgttt taaagaatca
    16741 ggattagaac aattaaaaaa agataattct ccagaattat tttctaattt taccgaagac
    16801 gacattccta ctcttaaaca attaaaatat tattctgatt attggacgca aactcgtgag
    16861 aatgaattaa atttagaaaa actcattatt aaaaagaaat agcaactcca gcccacacgg
    16921 ctggtttttt agtttcaact tccctcctta cgggtcaaaa tcatcggttc cggcataatc
    16981 tgatcatcct ttagcaacaa atcaggatat tttttcctta gcttcttatt gttcgtgtaa
    17041 gttttcaata gccattggtt ggcttcgctt ttgctttctc cagaaaaata aattttccct
    17101 gtttggatgc ctgcaagttg aatcatcatt ttcacctcaa atcaaatttt ttctgctaga
    17161 tggagcaaaa gcaatccgac atcaagaatc ttttgaccca ttatttcagc tactttgctc
    17221 ggcttaactc cttgagaaaa gagcaagcgt gcttgctcta tctcttcgtg agtccaaata
    17281 aatttggctt cctcaaggat aattactgga tcagttgctc tcattgttcc tctggaaaaa
    17341 tgatgtcatt cttatgcttg gaccaggtat ctgcatatgg aacaaagtat tgtctagcta
    17401 attcgatttg atcaattaaa gcagtttctg ataattcatg gtctgcagca atttcattca
    17461 tactatcgcc ttcatctaaa cagataagac cgttgacgtt atcgattttt acatttgtga
    17521 ctgcttgact ttctccacgc tcataatatt ctacagttga tactgataca tcagcttcgg
    17581 agtttaattg cagaatctgt tactgccggg ataatttaat attcttttca taattcacct
    17641 tttttcctac tgacatcgaa tatttttcat gttatgatta gccgtgaaag gagagctata
    17701 ttatgtgttt acacatgatg tccttagctc attcggttct ttggaagctt ttttggaaat
    17761 aactctcagg ttcttgacgc aaatctcatt tattctttga aacaaaaaac ttccattttc
    17821 cgagagcttt ggatcacttc aaaagagatt tgcatttata tttctgtgac cttgagtcgc
    17881 gtaaagaacc ttacagcaat ggcggcaatt ccactttcag gaaaccgcct tttttcattg
    17941 atattggtgg ttagcggact acccattttc tttcggcagt gccaaaccac aaatgatctg
    18001 tgttgatgtt taggtagttg tataccagat aagctaaatc caaagtgcct gcatcttcaa
    18061 gacgacaata ttccaaaaat tctcccatag ctggacataa aaatcctgga gctatcatat
    18121 cccaacagtt aatagtgcaa agaaattcgt ttagcttctg aacgttttct ttggtttcac
    18181 gaattacaac atactccaat tcttctccgc tggcagagca ttccaaaact tcaaattgat
    18241 taagaaatgg tagaacatat gcttcaaaca tcaacattcc atctttctgt ttttgcactt
    18301 gttttgcatc aatcatgtct atcctcctat tcagttattt cttccattaa tggaattatt
    18361 tcaggtcact ggacttaacg aaaacaccat tgaccatctt tccagtccga cctttgattt
    18421 cgtcataagc aaaatccaag cactcgtata aatccatatc attttgcatt gccaagatga
    18481 tcaaggttac tactacatcg ccaattccgt ctcttaaacc atgttcatct tttctagcta
    18541 gagaagcggc aacttccccg atttcttcga ccgtttttaa catttgcttg ctgaaatccg
    18601 cttgatccaa tcccttatct tttgcccact gctctacttt cgcgattagt tcgtccatta
    18661 tttctcctcc acatacctaa actgtcttcc ttttgaatca atccataagc tcctagctct
    18721 atcccaaata atatttttgc tcagaccagt aatttcagat aactgtttag cggtacccgt
    18781 tactagaatt cgatcaccat gccagattgc aatctttctc ggcgttctcc gtttgggctt
    18841 ttcagtccac attgatttac cgagcttttg gacttctgca actatttttt tatcttcctg
    18901 ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct tatccatccc
    18961 gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt ccgccatgaa
    19021 atgtgttgag ccacttgtct tcgtacgaca cactttgtct taaagcttct tgatgcatta
    19081 attcgatctg tgctgtaaat gtctttaggt ccatcttgtt tacacctgct caagttcatt
    19141 aagatgtttt tgcaagcctt taacacaatc aatgaatagt aatttggtat atgctaaatt
    19201 tcttaattgc gttccatcga tatagagtgc gaaatagtat ctgagtttac tccaactcga
    19261 acgatcattc ttaattcctt caattccagc ttcttcgagt tgatcatata cgtctctcag
    19321 gacgtctaat tcctcgccct ttttataatt cgctatttca ttaattagtt ctagataatc
    19381 gatcttcatt tttccacctc ttagaatggt gcttttgatt gtctattagc tcgttctagc
    19441 gcttttttct tgagataggc ttcttggtcg attgcccatt caggaagctt ctctcgtctt
    19501 cctgtgcgct tgtatccact gcttgcgttc ttaggttcac ttttttcttt ccttgcccaa
    19561 cttcgaatag ttgccaaata gtttttataa gtcttaccag atgattcaca atactctgac
    19621 agtcgttcga ttcgctcttg gtaatcatta gggaattctg ttttgagttt ctccatctgc
    19681 tcatctgaca aaagaacatt tttatactct ccgtatttat gacggacggg cttagccttc
    19741 gattttttcg aaggcggtaa ctctcttata tattcttttg tattattaaa tgtattatta
    19801 atagatgtat tattatcttt gactttttcg tcaatagggg tattgcgttt ttcgtcaata
    19861 gggtattgat taattcgtag gtacctattg attatttgat tggtaccctc tttgtaaatg
    19921 atttcccgat tcaagtatcc aaacttaatc aaatcactta cccatcgcga tatggtctct
    19981 ttattcacac catataaatc tgcaaagtac tcattgcctg cccaacaaaa gcctctttca
    20041 ttacacaagg ccgttatctc tccgtataac aacttagtat ttggtttaag tcttttgtcg
    20101 taccttacgt tggctggtat aatcgcataa taacttcgat gttctgtcat ttttcccctc
    20161 caatatttaa ctttttgatt gttttctggt ttaatttgat ccctttgatt tgatatttat
    20221 ttttgaaatt aatcacacct attttgtgct tctctgtgtg atggattctg cagagtgctg
    20281 caaatgtgta ctctgcatga tcaacttctt tgcgctttcg tcttcctagc gctttgtcaa
    20341 agtgatcgat gtcagctcct gttttgccac agatgcagca gactcttttt gtgatgcatt
    20401 tgtagaagta atattcttga ttcgcaggta aaatctcata gccttctttg aaaggaatat
    20461 gatgttcaaa gatgaaatct aagatgatat ttgctaagac attggcatca ctcacagttg
    20521 tattcgattc gtctttcagg cttattttgc gccctgtgac accttcaaaa cgaaagtaga
    20581 agaattcctt ccagaagtcc gttggcatgc ctgtatcgat aaaaatatcg cctatcagcg
    20641 catagatgaa gtttcgttgc tgtacggtga aacgtctagg atcaataaaa cgaatttcaa
    20701 tgactcgatc accatcgtag ccgtcataca tcgtctttag tcgatcaatg ttcacttcct
    20761 cattgatggt tgcgcttatg tcttttcctt tgaacttttt cagaaccgct gaatatgaat
    20821 cgattaatgg tttaaacact catatcactt cttatctaat tcttttctct tagctgctat
    20881 tgctcgctcc atcaaggcac attgctcata gcttaactgt tcaatagttt caacgttatc
    20941 agctaagagc cctaatttat ctgtctgctc attaacatat tcgattaagg ttttggtcat
    21001 atctttaccc atctgctcat tgaaagcttc tagaatcgtc tctagcatgt ttaatttctt
    21061 tgtatcgatt ctaggtggtg ttggaatatc ttccccttga aatacatata atcccagtcc
    21121 gtgtagagcc aatgctttca caaagcatcg cttcaatgag ttattgattt gcattgcatt
    21181 tggtttaaca actggttggt ttcgataatc taaaacagga aataattcgg tttccgtgtg
    21241 tcctttaacc gttactgaga cagatacata agtcccagtt tcatccataa gaaaaggttt
    21301 atattcctca acaagaaagt cttgatgagt tccagaaaca accctgtagt gtttgtactc
    21361 attaatagtt accgttgcct gtggatcatt ctttttcata atctcccacg cgtgagccca
    21421 agataaataa tcaaaatttc cttttttctt gagaatttta tttaacttac gactaaaaag
    21481 tttttcaaag ttcgttgtcc ctttgatttc actcatcaaa ttctgcctcc atttcagcaa
    21541 tgtatttctt acctggtccg taataagaga tatcaatcaa gttatctctg tcgtactctt
    21601 ctagcgcatc aatcaagcca tcttcgatga cgtaaatata ttcaggttta ttcgaatgct
    21661 tcgatagatg gataagataa acatgatccc aaatagttac atggttgcct aaatcatctt
    21721 gatcccaagc tagttcttca ttcgtcaaaa gatttcgtct gatttttcga ccacttgttt
    21781 cctcaatttt cggcttgccc caatcaggat caatcaaata ttgatctaga gtggaaagtt
    21841 ctttttccat atgctaaaat ctccttatga tgtgttttct ttgtgactct ttgcttgccg
    21901 gcggagtcac ttttttattt gttgccatgc tttttgcttg tcaatatgtt gttggcttag
    21961 gatgattggt ttatagtatt tccaccagca attagcaatt gccgtcccta ttcttagcgc
    22021 ttcagctcta ttcattgtca tcaccaaaaa gtctctgttg tctgttcagt tgatcaattt
    22081 ccatacggat cgcagtttct ggcaaccaca tttcaataaa tgaaacagca tcatcgaatc
    22141 tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat tcagaccaaa
    22201 attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt ccccctagaa
    22261 ctcccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt ccgctaattc
    22321 gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct tctaattgat
    22381 agatcatcat atcttcaatt gtttgaggaa cagtattctt gcgaataaca tcttccattt
    22441 cgttgaatgc ttcaatgtat ttttgtttga agtagatagc tttctttcct gtaaacccca
    22501 tagccagcaa gaaaaatcca tctctactaa tgaagaaaac tcgtcgattt ctgccatatg
    22561 aatctggttc attaccttct acaaacatct gtccaaaatt ggacacatct ttttttagtg
    22621 catcaatatc tcttaaaaca tgttgatgtt ttttctcgaa gctttctgc
//
LOCUS       DACDOA010000041        22565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDOA010000041 DACDOA010000000
VERSION     DACDOA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.041"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..111)
                     /locus_tag="IWI39_001422"
     CDS             complement(<1..111)
                     /locus_tag="IWI39_001422"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208536.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNV"
     gene            complement(302..1180)
                     /locus_tag="IWI39_001423"
     CDS             complement(302..1180)
                     /locus_tag="IWI39_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9208537.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1219..2337)
                     /locus_tag="IWI39_001424"
     CDS             complement(1219..2337)
                     /locus_tag="IWI39_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9208538.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2334..4433)
                     /locus_tag="IWI39_001425"
     CDS             complement(2334..4433)
                     /locus_tag="IWI39_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9208539.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4484..5305)
                     /locus_tag="IWI39_001426"
     CDS             complement(4484..5305)
                     /locus_tag="IWI39_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9208540.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5302..6111)
                     /locus_tag="IWI39_001427"
     CDS             complement(5302..6111)
                     /locus_tag="IWI39_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9208541.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6132..6626)
                     /locus_tag="IWI39_001428"
     CDS             complement(6132..6626)
                     /locus_tag="IWI39_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9208542.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6638..7126)
                     /locus_tag="IWI39_001429"
     CDS             complement(6638..7126)
                     /locus_tag="IWI39_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9208543.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7203..9899)
                     /locus_tag="IWI39_001430"
     CDS             complement(7203..9899)
                     /locus_tag="IWI39_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9208544.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10327..11400)
                     /locus_tag="IWI39_001431"
     CDS             complement(10327..11400)
                     /locus_tag="IWI39_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9208545.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11397..12224)
                     /locus_tag="IWI39_001432"
     CDS             complement(11397..12224)
                     /locus_tag="IWI39_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208546.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12225..13031)
                     /locus_tag="IWI39_001433"
     CDS             complement(12225..13031)
                     /locus_tag="IWI39_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208547.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13043..14128)
                     /locus_tag="IWI39_001434"
     CDS             complement(13043..14128)
                     /locus_tag="IWI39_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208548.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14145..14687)
                     /locus_tag="IWI39_001435"
     CDS             complement(14145..14687)
                     /locus_tag="IWI39_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208549.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            14945..15934
                     /locus_tag="IWI39_001436"
     CDS             14945..15934
                     /locus_tag="IWI39_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9208550.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15931..16668
                     /locus_tag="IWI39_001437"
     CDS             15931..16668
                     /locus_tag="IWI39_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9208551.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16665..17216
                     /gene="coaC"
                     /locus_tag="IWI39_001438"
     CDS             16665..17216
                     /gene="coaC"
                     /locus_tag="IWI39_001438"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9208552.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17232..17864
                     /locus_tag="IWI39_001439"
     CDS             17232..17864
                     /locus_tag="IWI39_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9208553.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            17949..18596
                     /locus_tag="IWI39_001440"
     CDS             17949..18596
                     /locus_tag="IWI39_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9208554.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18601..19584
                     /locus_tag="IWI39_001441"
     CDS             18601..19584
                     /locus_tag="IWI39_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9208555.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(19987..21501)
                     /locus_tag="IWI39_001442"
     CDS             complement(19987..21501)
                     /locus_tag="IWI39_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9208556.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21639..22445
                     /locus_tag="IWI39_001443"
     CDS             21639..22445
                     /locus_tag="IWI39_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208557.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6810 a         4841 c         3917 g         6997 t
ORIGIN      
        1 aacatttcga aaaatgaaca tacattggta agcaactata actattacca cagacataaa
       61 gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca aggtatacag
      121 cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa
      181 caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat ccaacttcat
      241 ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa acattcatta
      301 attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg attattccgg
      361 cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt tcaacatatc
      421 catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct tgtttcatta
      481 caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg tatgcacatt
      541 gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa ttctggcctt
      601 ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa caatcttggt
      661 ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac atatgcccca
      721 tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca cctccgatac
      781 ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca gcaacatatt
      841 caccatagca agccgcattg acatctgttg tccattcaat cggaatattg aaatattgct
      901 ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat gtgatatgac
      961 cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg
     1021 ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt tcaactggag
     1081 ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg actgcacaga
     1141 caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc tcccttcgaa
     1201 tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt
     1261 atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt cttgccgctc
     1321 aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat cttcggtaaa
     1381 acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg cgtcgtcacc
     1441 aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg catctctatt
     1501 tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa
     1561 ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac ctgttgaggt
     1621 tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt cgccccacca
     1681 ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt gaccctttaa
     1741 tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat gccaatttgc
     1801 gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta tcgtataaaa
     1861 aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg atgtttggaa
     1921 aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg gcaacgagtt
     1981 cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac tttcaacagc
     2041 agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa caaaactgcc
     2101 aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg ttccagtaat
     2161 atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac ctttgcggct
     2221 tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg gattttcggg
     2281 agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta tactcatctt
     2341 tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt tttcgtatca
     2401 cttattttca atggcttttc tgacaaattt gaaatactca actcggattg cgtacattca
     2461 atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa attatcaaca
     2521 ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact gatcattagg
     2581 ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg cacgacttct
     2641 ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa atctgccaaa
     2701 caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc gctcattaaa
     2761 acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat gcctttgatt
     2821 tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa
     2881 tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca
     2941 tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa gattaaccct
     3001 tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc ctcatcaccg
     3061 attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt acaaattgat
     3121 tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg ttctagggct
     3181 tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct gccggtatga
     3241 tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat tggttcccct
     3301 gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag aacctgcgga
     3361 caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc cagtaacgca
     3421 ctggataacg aattactcag agaatgattg atacctcctg taaattttga accaggaaac
     3481 ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg ttcaaacatg
     3541 gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt agcaggatag
     3601 ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact agaaaagctt
     3661 tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga cagctgccca
     3721 aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc agattcctga
     3781 aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa gcttgccact
     3841 tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt ttgctgatcc
     3901 gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt
     3961 tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc cagtatggga
     4021 gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct aggcaaacag
     4081 tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta gacaatcccg
     4141 tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt gacccaaaca
     4201 cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg aatcgtcccg
     4261 caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa tccctttaca
     4321 tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc gtcgtttaac
     4381 tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt catcttttcc
     4441 ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg attccaagta
     4501 atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg ccgacttttt
     4561 tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct ttaaaaatac
     4621 tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg
     4681 tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc aagatactag
     4741 cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata tatttagagc
     4801 ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt atattgaaca
     4861 taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg atcgctagtc
     4921 ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca gccaatggtc
     4981 ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct ttttcagaat
     5041 tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg
     5101 aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt ttgtagaaat
     5161 gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc accggattca
     5221 ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt ttctcatctt
     5281 tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc
     5341 atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt gtagatctct
     5401 ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca atggtaactc
     5461 gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt tactccaaag
     5521 catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga ctgttaatcc
     5581 gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg ctccaaataa
     5641 aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca taaagaagtg
     5701 cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat
     5761 cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata ctgctagtgg
     5821 aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga acgctgtagc
     5881 cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga cgacacccat
     5941 aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc
     6001 tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga acatttcatg
     6061 tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca taatttttct
     6121 ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc catcgccggt
     6181 gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc ttgaatttct
     6241 gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg cggtgtcatt
     6301 ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt cgcaactttt
     6361 accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc ccgcggatct
     6421 tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt cgatggtgca
     6481 gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct tgctactact
     6541 aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt
     6601 tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct tcctcttcat
     6661 ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat ttatttacca
     6721 gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg agcagaattg
     6781 gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag gttttggtaa
     6841 tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt
     6901 tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc acataagcat
     6961 taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta agaaagcctg
     7021 aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt cctttctttc
     7081 tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt ttaatcacgt
     7141 taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact tttttagaca
     7201 ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc ataagcattg
     7261 actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac ataataaaat
     7321 tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt ccgagccaag
     7381 aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac acggccattt
     7441 aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc actcatcacg
     7501 atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa gaattttact
     7561 agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa aatatgctcc
     7621 aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg aattgaaaag
     7681 gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata agtttcatca
     7741 ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc gatggattca
     7801 tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa acccgacata
     7861 caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac attgattgaa
     7921 tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt catcttcaag
     7981 ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc cccttttaaa
     8041 tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat cagcactaga
     8101 ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat ctctgacaca
     8161 ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa ttgattcact
     8221 actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc cttcagctcg
     8281 atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag aaagttctcg
     8341 tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta agtcgtccga
     8401 ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata gcttggggaa
     8461 atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa tccgtacgtt
     8521 tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt tctactcatt
     8581 gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt ctttatcgaa
     8641 gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc caaaatcgaa
     8701 tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt tatcaataaa
     8761 ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat caagatctca
     8821 tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc acaactgatt
     8881 ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa cattaaagac
     8941 aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt ttgaaaaaat
     9001 aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg caacataaca
     9061 ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt ttccgtcgtg
     9121 gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac tggacgatag
     9181 tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag gcgatggacc
     9241 tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt atccttttca
     9301 gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag ttcgggattg
     9361 tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat aatttgctca
     9421 tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc cgtggctcca
     9481 tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca tttttgaatg
     9541 acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt tatttgctcc
     9601 gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt tcccggtaat
     9661 ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt ctgacgagag
     9721 atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc tgttagtcgt
     9781 atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc cagtttaatg
     9841 tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt gtattccaat
     9901 ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa aataatttat
     9961 ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta caaatattga
    10021 actggattca tatattagtt ttattaatag attacagaag ggttaagtat atttgcatag
    10081 tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag cccgtctcgg
    10141 gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg tagaaaaaat
    10201 aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg aaaactcaga
    10261 tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc ttactgagtt
    10321 ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga tgcctaggta
    10381 tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag atggatagaa
    10441 ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat ttggtgtaga
    10501 ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa agttgatgaa
    10561 atcataggca ccttctttat ttttcacggt ttttggcata acgatattat caaaccaaag
    10621 attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca tcatatcggc
    10681 agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat acattttgat
    10741 ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat ttgctgcttc
    10801 tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca aagaaagccc
    10861 cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg gcttccacaa
    10921 atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata cgatccctaa
    10981 agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca agtctaaaaa
    11041 tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa gtactagttt
    11101 ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt agtttgtccc
    11161 accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat aattcacttt
    11221 gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat ctccccagtt
    11281 atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt gacgaacacc
    11341 aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta attttttcat
    11401 cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt tggacggttt
    11461 tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa aactaacgca
    11521 cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat ctctactgag
    11581 agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc aagagaatag
    11641 gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa aatgatattt
    11701 ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat tgaatcgttc
    11761 atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa agcaatatgg
    11821 gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa aatcaagaaa
    11881 ctagcaccga taataacatc aggagagact aataagatgt tattcatgct caacaacgct
    11941 gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt cccgatgatc
    12001 gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat aatgatcaaa
    12061 cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa gctgttcatc
    12121 gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata taagaataaa
    12181 aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga gcgacctcct
    12241 tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa aatcacaccg
    12301 attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat agccgttcct
    12361 aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga aagagaagga
    12421 ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa aatcactcgt
    12481 cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa agaaggattc
    12541 atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat atacgttgcc
    12601 acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc gataaaatca
    12661 aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta agctttaagc
    12721 agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt gtgcttcagt
    12781 tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt gatcaaaaat
    12841 gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa ataagataga
    12901 taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat gatcattaat
    12961 acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata gatcctgcgc
    13021 attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca tcgaattctt
    13081 cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc aatcctactt
    13141 tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc tgttcatcat
    13201 aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt acgactagtt
    13261 ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact tcaactggtt
    13321 catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac tcaactagat
    13381 tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca gcaacgaaat
    13441 gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt ttccctttat
    13501 tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga gtaacaaaaa
    13561 taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata tctgtacgta
    13621 gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt tcatttacaa
    13681 ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta atctcccgtt
    13741 tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt tcgatatctg
    13801 atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca ttcatatgag
    13861 gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct ggcaaatcat
    13921 tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat ccagctataa
    13981 tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa aattttcctt
    14041 gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt ttcactactt
    14101 gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa atatgattgc
    14161 gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg atgttgtttt
    14221 gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc tcctaagctt
    14281 aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg ttcaaacgtc
    14341 ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt ttcattcgaa
    14401 tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac cgtatgatct
    14461 tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc ttctggagtc
    14521 acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact taaatctcgt
    14581 tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc ttcttgtgtc
    14641 aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat ttcctccgaa
    14701 tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta tacgaaattt
    14761 attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta tcttttggaa
    14821 taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat ttacggaaga
    14881 acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa ggagtgtctc
    14941 acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc atacagcaat
    15001 cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa ttgcctattc
    15061 tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga tacgaacata
    15121 tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca atccacgttt
    15181 ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc atccaggcgg
    15241 ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa aaaatatgac
    15301 gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt gtgtgaacgc
    15361 tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg cttctggcgg
    15421 tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa tcattacctt
    15481 ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc aagtcgtacg
    15541 acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa tcgactatgt
    15601 catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct cttataacgg
    15661 tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg tcttgccttt
    15721 tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa tagatcaacg
    15781 tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg tcgtccaaga
    15841 gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa cacaccaagg
    15901 aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc tggaggaacg
    15961 actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg attagggcaa
    16021 gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt gactacgaaa
    16081 caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga atctgcactg
    16141 gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc gattatccac
    16201 agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca gctggcaaaa
    16261 aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga aaatgaacag
    16321 acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct cgttttgaag
    16381 aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa aatcattggt
    16441 ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa aaatagtctg
    16501 ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca tggcgagaca
    16561 caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag taaatctgag
    16621 attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa aagatcttac
    16681 taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt caattcgtca
    16741 aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt atcacgccat
    16801 taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa gaatcaaatc
    16861 cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc atcgcgcctg
    16921 ctacagcaaa cattatcggc aagctagcaa acggcattgc agacgatctt ctttcaacag
    16981 tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg aacacgaata
    17041 tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc ggctatcagg
    17101 aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt gccttagctg
    17161 atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat gcttagaaaa
    17221 aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt ttagctattt
    17281 tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc aatgcaacaa
    17341 ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta ggtgcttttc
    17401 ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa acacctttgt
    17461 cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc tggaaagcct
    17521 tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc gtttataaag
    17581 gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta gctggacttt
    17641 ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta ttccagcaag
    17701 attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt ctagcggtta
    17761 tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca gcagtatgca
    17821 atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca tcgtagccga
    17881 tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac gtcgaaagga
    17941 tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga cacctgataa
    18001 aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt gcttcccaaa
    18061 cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg tccgtggaaa
    18121 aaatgaccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc tctcaaggaa
    18181 agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca gctgaaaatg
    18241 cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca gcaaaatggc
    18301 ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg ctgaaacgaa
    18361 ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa gaagttactt
    18421 ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc tctacagttg
    18481 ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact ctagtacttt
    18541 ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag gaatagatcc
    18601 atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca aattgaagtg
    18661 cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc agctgtcaat
    18721 cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc tattttggga
    18781 gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt tactcctggg
    18841 acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt gatgcctgcc
    18901 gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg aatctcagaa
    18961 gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga aggcgaaact
    19021 gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt agctagacag
    19081 ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc cttttgtaaa
    19141 gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga agaggtacga
    19201 aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc ttcttactgg
    19261 gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg tatgctaggt
    19321 gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt ccaactgctt
    19381 ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca aacttttatg
    19441 caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga ccgtacattc
    19501 caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa catcggaaaa
    19561 atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg ggacagaagc
    19621 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    19681 tcagcttatt tccgaaggaa tttcttctgc ttccgccgtt tatacgtgtt tagagcgtga
    19741 gacaaaagtg ttctttactt ttgtcccacg ctctttttcc gaataaacgg tgttcaaaag
    19801 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagaagctgt ttttacttgg
    19861 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttcactaa acagtgttca
    19921 atcagctgac cctcggtatt agctcaaaac gctgatttca caacctcttt ttatttacgt
    19981 tctatttcat tcattttccg aagcaaatcg agctgcttgt atgcccgctt gtcgcccaaa
    20041 aataatgatc tccgcaactg aatttccacc gatccggttg tttccatgta agccacctgt
    20101 gacttcacca gcagcatata accccctaat tggctggttt tctttatcta atacttcagt
    20161 gttcgtattg attttcacac cgcccatcgt gtaatggatt cctggtgcga ttttgatcgc
    20221 ataataattc ggagacgata agtcatgttc catggctgtg gcacggttga attgagcatc
    20281 ttttttctcc gcaacatccg tattccatcc atcaactgtt tttgctagtg tttcttttgg
    20341 taaatcgatt ttttctgcta attcttccat tgtctttcca gttgtgacat atcctttttg
    20401 gtcataaaat tcaatagctg tggcacgatc acgcacgcct tggtcaaaaa tcaaataagc
    20461 tgatttttca ggcaatgctg taattgctgc agatacttta tcccttgtat ccatttcatt
    20521 gacaaatctt ttcccctctt gatcgaccaa gattgctcct tctccgcgaa caacctcacc
    20581 aatcaagacc ccctcatcct gctgcacagt tggatgaatt tgtattttat ccatatcaac
    20641 ggtttgtcca cctaattttt caatcatggt gataccatct ccagttgatc cttcttgatt
    20701 tgtcgtaata taatcagcta aatcgggacg atatttttca ataaattctt tgctggcacc
    20761 aaaaccgcct gtggtaacta cgattgcttt tccggtgacc tcttttggtt cttctccttg
    20821 aacatgtaca gtcacttcat tgaccgtttc cccatttttc ttaatgtctg tcacatctgc
    20881 gttgacaaaa atagggattt cccgctcatg gacgttattc aataacccct ccactaagta
    20941 tccaccaata gccgagccat cagacggacg atgtgtcctc ttttcactca tccctccagt
    21001 gatcgttaag ttgtccagtt tgataccgtt ttgatctagc caatcgaccg catcagcaga
    21061 gtgatcgaca aagtatcgaa gcatttcttt atcattcgtt cctccaccac cttttagcgt
    21121 ttcttcatag aaggcatcat tcgagtcttt gattccttct tctttttgaa attttgtttc
    21181 tgaagcattc ataccgcttg atgatttaga tgtgtttcct ccggcaacag gcatcttttc
    21241 caaaattact ggattcaacc cagcatcttt tgcttcgata gctgctgcca tccctgcacc
    21301 accagctcca ataatcacga catcataaga ttctttcatt tcttttggat ctgtatatac
    21361 ttgttcagaa gcgcctgatg atacttcggc agattccttc gtactagaac ttgtctgcgc
    21421 tgtatttttc tgatctgcgc cgcatgcgct catagcaaga agtgcaacgg ctgttaataa
    21481 accagcaact aattttttca tttgtatccc ccttttctcc ttatactata ttaagtgatt
    21541 tttttcacaa tatcaactct gtttttcttt aagtcatata gctttggtaa acacacgctt
    21601 cttagtataa ttaaggatat acatgaagga gttattttat gaatcatttg aaaacctttt
    21661 ttacgaacag tacaaaaact gcaggtagaa tcacaaacgg aatctatatt ttattacgta
    21721 tcatcgtcgt tattatgcta gtggatcagg tctggaacca acactgggga aatacaatgc
    21781 ttttattact gacattaatt ttatttgaaa tcccttattt tctggaaaag atattaaaga
    21841 tacagattcc aaatatgcta gaaatcattt tgttatcttt tatttttagt tcaacagttt
    21901 taggagagtt aagtgatttt tatggctatt tttcaatgtg ggatactttt ctacatgcat
    21961 tcagcggatt tttagctggg ggtgtcggct tttcccttgt ttatttgctc aataaaaata
    22021 caaaaaccat gaacttgact cctttattac tagcagttgt ttccttctgt ttttcgatga
    22081 cagttggtgt tatgtgggag tttgtggaat tttcagcaga taaattactg caattcgata
    22141 tgcaaaaaga tacgatcatc cagcaaatca atagtgtgga gttaagtaaa gacggaaaca
    22201 ctgtctatca tgttccagat atcactcgga caatcattga aagtcaaaat gcagatggta
    22261 aaacagataa aacaatcatc caaaatggtt acttagacgt gggaatcatg gatactatga
    22321 aggatttatt tgttaatttt attggagcag ttgttttcag tgtatttggt tacctctata
    22381 gtcgaaacga ttaccaaaaa tttacgttca tcaagaattt tattcctaaa aaaatcaaac
    22441 gataaaacat gctaaaaaac aagagaaggg gcagtgacaa tacttttgtc actgcccctt
    22501 cttttttgaa tgtatggcat gccattagtt atccgatagt attaactcaa aaaagtaaga
    22561 tatga
//
LOCUS       DACDOA010000042        22087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDOA010000042 DACDOA010000000
VERSION     DACDOA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.042"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(58..1446)
                     /gene="ltrA"
                     /locus_tag="IWI39_001444"
     CDS             complement(58..1446)
                     /gene="ltrA"
                     /locus_tag="IWI39_001444"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9208558.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1952..2479)
                     /locus_tag="IWI39_001445"
     CDS             complement(1952..2479)
                     /locus_tag="IWI39_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9208559.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(2546..4048)
                     /locus_tag="IWI39_001446"
     CDS             complement(2546..4048)
                     /locus_tag="IWI39_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9208560.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(4306..5427)
                     /locus_tag="IWI39_001447"
     CDS             complement(4306..5427)
                     /locus_tag="IWI39_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9208561.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5553..6494
                     /locus_tag="IWI39_001448"
     CDS             5553..6494
                     /locus_tag="IWI39_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9208562.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(6644..7540)
                     /locus_tag="IWI39_001449"
     CDS             complement(6644..7540)
                     /locus_tag="IWI39_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9208563.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            7809..9149
                     /locus_tag="IWI39_001450"
     CDS             7809..9149
                     /locus_tag="IWI39_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9208564.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            9367..9810
                     /locus_tag="IWI39_001451"
     CDS             9367..9810
                     /locus_tag="IWI39_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9208565.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(10314..11732)
                     /gene="nhaC"
                     /locus_tag="IWI39_001452"
     CDS             complement(10314..11732)
                     /gene="nhaC"
                     /locus_tag="IWI39_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9208566.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(12135..13358)
                     /locus_tag="IWI39_001453"
     CDS             complement(12135..13358)
                     /locus_tag="IWI39_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9208567.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(13389..14228)
                     /locus_tag="IWI39_001454"
     CDS             complement(13389..14228)
                     /locus_tag="IWI39_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9208568.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(14245..14925)
                     /locus_tag="IWI39_001455"
     CDS             complement(14245..14925)
                     /locus_tag="IWI39_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208569.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(14927..16000)
                     /locus_tag="IWI39_001456"
     CDS             complement(14927..16000)
                     /locus_tag="IWI39_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9208570.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(16647..17318)
                     /locus_tag="IWI39_001457"
     CDS             complement(16647..17318)
                     /locus_tag="IWI39_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9208571.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(17405..20083)
                     /gene="mgtA"
                     /locus_tag="IWI39_001458"
     CDS             complement(17405..20083)
                     /gene="mgtA"
                     /locus_tag="IWI39_001458"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9208572.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            20742..21179
                     /gene="ndk"
                     /locus_tag="IWI39_001459"
     CDS             20742..21179
                     /gene="ndk"
                     /locus_tag="IWI39_001459"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9208573.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(21201..22067)
                     /locus_tag="IWI39_001460"
     CDS             complement(21201..22067)
                     /locus_tag="IWI39_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9208574.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         6203 a         4681 c         3922 g         7281 t
ORIGIN      
        1 ccaactgacc ttccgcccct ctcacaccac cgtacgtacg gacccgtata cggcggttca
       61 ataagttgag tgtacgaact ggtacttcaa ggagatgtcc tcatatcctt ggcgggccag
      121 ttctttgtct gttaatgatc gatgaagaat atgacttttg gagactgacc aaataccttt
      181 tcttgtattt gaccattcat acgctttcgc tttatcgatc cctaaacgaa tcagattttt
      241 tcttttggtt cttggattct tccattgttt ccaaatatat tgtctgattc ttcgcttcaa
      301 ccaaccgttt aactccctca tgaattcttt catctcacca attccatagt agttgatcca
      361 accagtcatt agttgcttga tttcttttaa aataagttgg atacttcttc cacggtttct
      421 tttagtgagt tgtttgagct tgcttttcac acgctgtttt gcttttgcgt gagggcgaat
      481 tttaatacct ttctttgttg gcagaatgca gaatccaaga aactttcgtt tcaatggact
      541 tcctaccgca ctcttttctc gatttaccgt caactttagt tgttcttcga gaaattgaat
      601 ggatttcttc atcacacgct gtcctgctcg tttgcttcta acatagatat tgcagtcatc
      661 ggcgtatcga acaaatttat gtcctagatt ttctagcact tgatcaaatt cgttcaggta
      721 gatgttactt aacaggggag agagattgcc tccttggggc gttccttctt cagttgttgt
      781 gatgagtcca ttttccatta caccactcat taagtattga cggatcagtt ttaggacttg
      841 tttgtccttt actttccgct caatgaaata catcagctta tcgtgattta cggtgtcaaa
      901 gtaggctttc atatcaatgt ccaccacata tcggtaacct tgttcataat aagattttgc
      961 tttcttcact gcgtcatgtg cgctgcgatt tgggcgaaac ccataactat tatctgagaa
     1021 ttctggttca aatagcggtt gtaacacttg attgattgcc tgttgaagca tacgatcaat
     1081 aaccgtggga attcctagtt ttcttacacc gccgtttggt ttcggtattt ctactcgcaa
     1141 aacaggctga ggtttatagt tcccttggcg taaattggat agtaattcct cgcggtgctc
     1201 tttcaagtaa ggaagcagtt gatcgacggt catcccatct gcaccagccg cccctttatt
     1261 tctgactact tgaagatacg cttgattgag attattccga tctagaattc tcccaaacag
     1321 attttctaca ccatcttgtt tcctctgttc accgcaagaa gtgctgcgca cttcatcata
     1381 cccttcgtgt tccacactag ctctttgatg atagccaagt tttcttgttt tctgcggtcg
     1441 tcgcatcttc tcacacctcc taaattattc aagattctta ttgttcagtc cttcgtaacg
     1501 gtcagtgtcg ttactactat gacctcggct gacttctcat gattcaccat catgtcgcca
     1561 tgatcgttgt gctggtttgt tttcattcaa cgcccacaac accctcatga gatctccccc
     1621 ggtaagagtg aaaactttcc tcccatgtaa ctgctagatt tactgtatag gtttcgggca
     1681 gtattggact ttgtcttgtg atgaagactt atccgaccta attcagcctt gtatctagtt
     1741 tctgttcgtc agttcaggag tttgcgtccg acttccttca gccacaacct cacgattgcc
     1801 gccttgtctt tcgctaacag ttcctactgc caagcctgta gtggactttc accaccaagt
     1861 tttcacccat gcagggcgca cacctaaaaa aggcactgct aagcaaaagc agtgccttga
     1921 aatattttat ttaaataaca caaaaacata attactcata agaaaccaat tctgtattat
     1981 ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca ttgatcactc
     2041 ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg atgcattctt
     2101 tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt gggaatcgta
     2161 cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt ccatcataat
     2221 ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg agacgatcag
     2281 caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt ttcgtgattg
     2341 gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt cctttttcag
     2401 tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt gtttctccgt
     2461 gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc ttaaccatac
     2521 cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc tcagtagata
     2581 ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc acgttcaaga
     2641 tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt tcatcccatg
     2701 tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc cgttggccca
     2761 tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc tttcggaggt
     2821 tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc aacgttcctc
     2881 gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc tggtattttt
     2941 ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat gactttcctg
     3001 caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa tgaattggct
     3061 cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt cgcttatggt
     3121 gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt aatagttttt
     3181 ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt ttcatcgtcc
     3241 gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta tcgatgaatc
     3301 cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct ttttcagctg
     3361 ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc atttcaaatt
     3421 catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa agttccagtt
     3481 gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc cggtcatggc
     3541 tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt tttctaccag
     3601 agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca tccacaaata
     3661 aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat tctctccata
     3721 agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat atctcaaaat
     3781 ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc tgttgcggga
     3841 aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg ttttgtagaa
     3901 tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg
     3961 tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg
     4021 tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa gaccagtcct
     4081 attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag acccatacat
     4141 cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc acaaggtaca
     4201 gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc ctcactttgt
     4261 cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact tcgggaggat
     4321 ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg
     4381 ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa ttgggagaaa
     4441 atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta ttggttttcg
     4501 atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg tttgctgatc
     4561 gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt ccaatccagt
     4621 gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa aaagggctcc
     4681 accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct cccagaaatc
     4741 ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg tcgtatctat
     4801 tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat atggattggt
     4861 aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc gatcaaaatg
     4921 aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag aagcacccaa
     4981 ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag tgacaataat
     5041 ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat aactggaagc
     5101 aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg cttgtgcaga
     5161 ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga tttttcgctg
     5221 acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg cgtaatagat
     5281 tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc ttcctttacg
     5341 atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa
     5401 tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta agtaagacac
     5461 tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct atatactaaa
     5521 agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct ggggccggag
     5581 caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg
     5641 atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct gactttaacg
     5701 gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc gaaagcaacg
     5761 aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat atgttttgtt
     5821 cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat ggattaggtc
     5881 atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg atcacgatgt
     5941 ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga gaaatcgaac
     6001 tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag
     6061 aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg cgaaaagctt
     6121 gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt gctgaactag
     6181 gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt gcttctatcg
     6241 cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt gcttctactt
     6301 atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg atcaaaaacc
     6361 atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga caaaaatatg
     6421 atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa gaaggaatcc
     6481 ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct ggttctcttt
     6541 ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca taagaattgt
     6601 gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg ttttcaacag
     6661 aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata aatcgttttg
     6721 cccaattttc tgcatcataa aattccaata gctctgatag gattggctta aatggtgtat
     6781 gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata ttttcaggac
     6841 gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt tgaaaataca
     6901 tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt tccagaatcc
     6961 cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca ggttgttcat
     7021 tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata aattcctgct
     7081 tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat agatccgtct
     7141 ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc
     7201 caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt
     7261 gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa aataacacaa
     7321 tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat ccataatcta
     7381 aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg aattccgact
     7441 gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa aaataaagtg
     7501 atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc tcactttctc
     7561 tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat
     7621 atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat tcttctatta
     7681 aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc
     7741 attgttaaat attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga
     7801 ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt
     7861 tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga
     7921 tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga
     7981 tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt
     8041 tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta
     8101 tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt
     8161 tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca
     8221 atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca
     8281 gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca
     8341 caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta
     8401 tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga
     8461 ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag
     8521 gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt
     8581 ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa
     8641 tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat
     8701 ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa
     8761 aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt
     8821 tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat
     8881 tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca
     8941 gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct
     9001 tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg
     9061 gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga
     9121 atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg
     9181 gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt
     9241 ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat
     9301 aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg
     9361 ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg
     9421 tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga
     9481 gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg
     9541 aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt
     9601 gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag
     9661 gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat
     9721 cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa
     9781 atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat
     9841 gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata
     9901 aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
     9961 ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta
    10021 ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag
    10081 ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt
    10141 atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg
    10201 ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt
    10261 tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt
    10321 tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc
    10381 caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat
    10441 ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc
    10501 caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt
    10561 ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat
    10621 aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc
    10681 aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca
    10741 aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc
    10801 atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa
    10861 acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata
    10921 aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt
    10981 ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag
    11041 tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg
    11101 tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc
    11161 atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat
    11221 tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc
    11281 agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac
    11341 ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa
    11401 gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc
    11461 cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg
    11521 attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt
    11581 atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc
    11641 tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct
    11701 tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt
    11761 atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct
    11821 ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta
    11881 attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg
    11941 taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc
    12001 taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt
    12061 caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac
    12121 aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga
    12181 tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg
    12241 gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct
    12301 ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt
    12361 gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc
    12421 gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca
    12481 aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc
    12541 gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct
    12601 aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg
    12661 ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc
    12721 aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct
    12781 ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca
    12841 ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca
    12901 tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc
    12961 ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc
    13021 ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga
    13081 ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg
    13141 gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac
    13201 ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact
    13261 tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt
    13321 agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt
    13381 catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt
    13441 ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt
    13501 ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca
    13561 tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca
    13621 ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct
    13681 aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt
    13741 ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg
    13801 ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc
    13861 ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa
    13921 tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa
    13981 tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat
    14041 tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct
    14101 tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct
    14161 tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt
    14221 tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat
    14281 aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt
    14341 ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc
    14401 tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat
    14461 accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat
    14521 cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg
    14581 agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt
    14641 cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat
    14701 ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa
    14761 tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat
    14821 gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg
    14881 aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt
    14941 cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag
    15001 atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga
    15061 tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg
    15121 ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat
    15181 aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga
    15241 tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt
    15301 cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca
    15361 taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga
    15421 ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt
    15481 tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag
    15541 ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg
    15601 ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt
    15661 tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca
    15721 ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg
    15781 gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca
    15841 gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat
    15901 aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt
    15961 tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa
    16021 aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta
    16081 ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag
    16141 caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg
    16201 gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt
    16261 tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta
    16321 acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc
    16381 cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag
    16441 ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac
    16501 ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca
    16561 acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta
    16621 cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata
    16681 acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta
    16741 taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca
    16801 gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt
    16861 cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg
    16921 aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt
    16981 ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat
    17041 atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat
    17101 tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa
    17161 atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata
    17221 tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca
    17281 actaacaaac catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa
    17341 acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc
    17401 tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg
    17461 ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca
    17521 agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg
    17581 tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac
    17641 gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga
    17701 ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta
    17761 cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg
    17821 ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt
    17881 ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca
    17941 gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa
    18001 tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga
    18061 tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt
    18121 ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac
    18181 cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag
    18241 tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta
    18301 cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt
    18361 tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa
    18421 ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc agagctttca
    18481 acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca
    18541 tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga
    18601 caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt
    18661 ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca
    18721 gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga tcagcaacca
    18781 caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga
    18841 tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt
    18901 tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat
    18961 ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt
    19021 tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca
    19081 ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg
    19141 gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc
    19201 aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt
    19261 tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt
    19321 cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc
    19381 cttccgcact tccactgatg acattacttc ccataaagac aagattttcg taacttgttt
    19441 cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg
    19501 tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta
    19561 tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct
    19621 ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt
    19681 cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca
    19741 tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt
    19801 ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg
    19861 ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt
    19921 aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata
    19981 gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt
    20041 tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt
    20101 ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag
    20161 ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt
    20221 ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc gaatagttat
    20281 ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa
    20341 aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag
    20401 atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac
    20461 cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt
    20521 tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt
    20581 cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt
    20641 ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc
    20701 tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta
    20761 ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag
    20821 caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag
    20881 aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt
    20941 ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga
    21001 tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc
    21061 tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga
    21121 tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat
    21181 acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct
    21241 aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag
    21301 tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc
    21361 aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca
    21421 ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt
    21481 aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt
    21541 ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct
    21601 agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt
    21661 cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg
    21721 tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg
    21781 ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt
    21841 ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc
    21901 atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt
    21961 gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc
    22021 aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt
    22081 tttaatt
//
LOCUS       DACDOA010000043        21732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDOA010000043 DACDOA010000000
VERSION     DACDOA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.043"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(175..1086)
                     /locus_tag="IWI39_001461"
     CDS             complement(175..1086)
                     /locus_tag="IWI39_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9208575.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1463..2101)
                     /locus_tag="IWI39_001462"
     CDS             complement(1463..2101)
                     /locus_tag="IWI39_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208576.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2105..2875)
                     /locus_tag="IWI39_001463"
     CDS             complement(2105..2875)
                     /locus_tag="IWI39_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9208577.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3293..3709)
                     /locus_tag="IWI39_001464"
     CDS             complement(3293..3709)
                     /locus_tag="IWI39_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208578.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3760..4141)
                     /locus_tag="IWI39_001465"
                     /pseudo
     CDS             complement(3760..4141)
                     /locus_tag="IWI39_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4267..4932
                     /locus_tag="IWI39_001466"
     CDS             4267..4932
                     /locus_tag="IWI39_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208579.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5329..7362)
                     /gene="brxL"
                     /locus_tag="IWI39_001467"
     CDS             complement(5329..7362)
                     /gene="brxL"
                     /locus_tag="IWI39_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAP9208580.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7480..10002)
                     /gene="pglZ"
                     /locus_tag="IWI39_001468"
     CDS             complement(7480..10002)
                     /gene="pglZ"
                     /locus_tag="IWI39_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAP9208581.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10066..11192)
                     /locus_tag="IWI39_001469"
     CDS             complement(join(10066..10964,10964..11192))
                     /locus_tag="IWI39_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9208582.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11596..12378)
                     /locus_tag="IWI39_001470"
     CDS             complement(11596..12378)
                     /locus_tag="IWI39_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208583.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12443..13279)
                     /locus_tag="IWI39_001471"
     CDS             complement(12443..13279)
                     /locus_tag="IWI39_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9208584.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13297..14706)
                     /locus_tag="IWI39_001472"
     CDS             complement(13297..14706)
                     /locus_tag="IWI39_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9208585.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14720..16543)
                     /locus_tag="IWI39_001473"
     CDS             complement(14720..16543)
                     /locus_tag="IWI39_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9208586.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17386..17712)
                     /locus_tag="IWI39_001474"
     CDS             complement(17386..17712)
                     /locus_tag="IWI39_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208587.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17758..18120)
                     /locus_tag="IWI39_001475"
     CDS             complement(17758..18120)
                     /locus_tag="IWI39_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAP9208588.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18157..18371)
                     /locus_tag="IWI39_001476"
                     /pseudo
     CDS             complement(18157..18371)
                     /locus_tag="IWI39_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18499..18834
                     /locus_tag="IWI39_001477"
     CDS             18499..18834
                     /locus_tag="IWI39_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208589.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18910..20907)
                     /locus_tag="IWI39_001478"
     CDS             complement(18910..20907)
                     /locus_tag="IWI39_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208590.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21005..21280)
                     /locus_tag="IWI39_001479"
     CDS             complement(21005..21280)
                     /locus_tag="IWI39_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208591.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21512..>21732
                     /locus_tag="IWI39_001480"
     CDS             21512..>21732
                     /locus_tag="IWI39_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208592.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNM"
BASE COUNT         6847 a         4300 c         3192 g         7393 t
ORIGIN      
        1 ttaagattat acagaaaaat atctagggtg gctggattct aagtaaaaaa aatttttaaa
       61 ataaaaagaa ctaaatcaag taataattaa actactgttg atttagttct aataaaaaga
      121 tttagatgaa cagttttatt gatttaaata gttcatattt tttatttata ccgcttattt
      181 actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa agtctttgtc
      241 tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct ggaaggtact
      301 gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt tatccatgtc
      361 gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag tataagttga
      421 agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa aggctgcttc
      481 tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc cgccatcttc
      541 ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt ctcccttatc
      601 agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa tttgagcttc
      661 cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca atttgatatg
      721 tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga atgtttgttc
      781 tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt tctttgtttg
      841 atcatattgg gaatcgattt ctttggttga tacgtcatct ccatatttat cttcaaatac
      901 ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt tcaagctatc
      961 cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt tttcaccggc
     1021 attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta aaaatctttt
     1081 tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac aaaggtgttc
     1141 aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg aatagatata
     1201 aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca catcacacct
     1261 taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg tatttaacgt
     1321 aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg ttgggatatt
     1381 caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt tattagaaaa
     1441 agtttgtttt ttcatttgtt ttctaaagca tgatcttctt gtttcctatt ttggtcagat
     1501 atggatcatg cgtgaccacg ataactaacg taccttggga agcctgttgc gataagattt
     1561 caacgacttg ctccttattt cgtttatcta atgatccagt tggttcatcg gctaaaatga
     1621 ttttgctatt cttcaataag actctcgcac aggcgattct ttgcttctca ccaccagata
     1681 atgtgtagat aggcgtactt agcttcttgt ttatatttac ttttttcaat gcctcatact
     1741 ttgctgttcg cttatctgaa acatttaaat tcgaaaactt caatccaata tctaaattaa
     1801 aatcgattgt ttcatattca atcaacccaa aatcttgaaa aagaaaagag agggcatttc
     1861 tttttatcaa gcgcttctct cttccagtta aatgttttgt ttcttgatta aataaataaa
     1921 attgccctga actgaattga tcattcaatc ctaatatatt caataaagta cttttccctg
     1981 ctccgctctc tccaactaca gtcacaaaat ccccagcatc cgcagtgaaa cgatccaaat
     2041 gaagaatcgt tttgtttcga atgatttttt tcgcaccttt tatcactatc aacggctcca
     2101 attgttaacc tccattcatc agcaataact gacttttctt ttctgtagac aaaataaaaa
     2161 caactgaaag aataaaatca atgaagctaa agaaaaaaat attaaaaaga tatttaggct
     2221 ctctttctat aagaaaaagg aagattacac ttgtcgttcc ccatttgatg aggttcatca
     2281 tgaagaaatc tttatacgtt tcaaagagcg aataaccact tgaacgaaag atagcgattt
     2341 tccgacaatt gtagacaaaa tatatgcata acatgacata gctcaacaag aagaaagaaa
     2401 agaaagtgag taaaaatatc tgtatcaagt aatgaaatga tctgcttaac actttgacag
     2461 ctatctcttc agtctgagta tacggaaatg ataactgcaa gttatcacgt aactgacttt
     2521 cttcaatcag ctcaaataac ctttctttcg acaaagagcc tatcttgatc tttaacggtg
     2581 gatagatttc tccgctgaga atatttcttt cccaatacgt actgtttttc aatgtcagta
     2641 tttcaacaac cggataatgt tcgatccagg ggatagaagg aataaatgta taaatgggtt
     2701 gattgttttt tataaataga aatttcggtt ctttttcact gatttcttga taatattctg
     2761 ttatttgcgc caggtcattt tttaaatctg tatgtctata tcgttcaggg atcaatataa
     2821 cagggtgcgt ttctttttcg gttatttgga tcaaccttcc agagacatca atcaaaggaa
     2881 atttcttaag ataattcaga tttattttta tccctctgcc aaactgttca ttttcttcat
     2941 tcaagtaatc tgatacattg actaatatac ttccgttttt attcaaagtt tcatataatt
     3001 cctgatcggc tgctgatgca aaacgattgg aataaataaa atccacgtgg tttttcccga
     3061 tggttactgg ataaaataca tgatagtcct caccagcgat ttcttttggt gtttcatggg
     3121 aaacagctaa ctgtgaagaa gaaaaaacga tagttgctaa ttcgacattg gcaacggtca
     3181 aaagaagaga aaacaacaat ccaaatgtaa aaagaatgta ttggatatag ttgaacgctt
     3241 ctcttaacga agcgattttt tcaactaata aaagagaaat caaagagata ccctatattc
     3301 cgctgaacaa gaaaacgtac atctggaaca aataaagaaa ttgctcctta taatacataa
     3361 aaataaaacc accaatgatt cctgcaatta tgaatgtaat aatgattttc tttttcccca
     3421 ataaacacca caatacttga tgtaatgaaa agccattatt caacaataaa ctgatttctt
     3481 tattcatcaa taagaaaaag gatgataaca aaaggaataa aagaatcaat ccaatagtta
     3541 aatacactcg ataattatat acgtctgtac ttgaaataac cgaatacgaa tagctatttt
     3601 caaagtcttc aaaaatatta gtggcataat cagtgtgaaa tgcttgattg aatcgttcca
     3661 caaatagtaa aatgaaccgt tcataggctt ctttttctct cgtcatcaag aaaaattcac
     3721 cttcaaaatc ttttattttg atcgattctt caatggacgt tacgcgaaag gaggtattag
     3781 aatagggtac cttctcgtga gaagatttga catccccttg ataatacaat gtcatttcaa
     3841 gctttggctg tatcttgaag ttccaagatg tatctacttc attggattca tttactgttt
     3901 ttttcatata agaaagctca gtttctgatg agacattttc aaataccatg attagctgct
     3961 catattcttc ttgcccaata aaatgttgtg gcaatcgaat cggagtataa gtatctgttt
     4021 gatggaaaaa ttcatttgtc cgatagagta cctgtttatt tataataacc aaggcaaaac
     4081 cactcatgat accggtcact atgattgtga gtaaaactgt gaactttttt ttgattatca
     4141 ccactctccc ttacttaaat gattctttga taaaatcata ccgtttttca tcaaaaaaac
     4201 gtatctatca aatgtatagg tcatgtagaa taaatctata tactgtagaa tcgaaaggag
     4261 aagaagatga acatcggaga aaaaatacga acttaccgca gaaattgtaa gcttacgcaa
     4321 aaagaattag gcgaaaaaat cggtgtttcc gataaaacaa tctctagttg ggaaaattct
     4381 cgtacaatgc cagaccttga aatgctgtca cttttacatc agactttagg tctgccgatt
     4441 gattttcctt ctattgaatc agctaccgaa tcaatagaac caacattgcc ggatgaacct
     4501 cttgttatca agaaatctta ttcatcaaaa caacccttct cactcaaaaa gacactatct
     4561 ttctttctgc tcgtcttttg tttggtctat ctttttctta tcaattatca atataaaaat
     4621 gaaacggttg atcgattcaa tccttttttg aaaccacaaa tcggctatac cacactccct
     4681 gtaggatacg tggaaaattc catcgaacat tgggtatgta atgatatctt tgttcatggt
     4741 acatggctgt cttttcacgc tggagaggta gaaaaaggta agaatcatgc tgtaatcaaa
     4801 cacaaaggaa aacacgtcat gaacgttcgc ctgctcactt gggaagccat tccctcagat
     4861 taccaagaaa tcctcatcca agactatgac gatgccttaa aaagaagccc tcaggagcca
     4921 gataggcatt aagtaggaga tgaacgtaga gatagaaaaa atcaatgcac atgatcttat
     4981 caaaaaaaaa caggggtaca aattaattga aaaagctttg agatgaaagt tcctaaataa
     5041 aaaagttcag aatgataggt aaaaaaatga ctaactattc tagtattagg ctatttgtat
     5101 tattcttctg ttatttttat atcaaaaaat ccagaaataa ctaaatatag gtatttctgg
     5161 cttatagtat aatatttacg ccacagcccc caatgattct ttttggaatc atgatcaagg
     5221 acggacatac acaaaaatat tttgcccatt cgatccgaat agattaacga ctaatctagt
     5281 aagtaatata gattggtacg tagtcaaacc agcaaatgga aaagttgtct attccacccc
     5341 aagggcttta aagacagcat cttccggcgt ttgataaaaa atcaaattaa aggtacccat
     5401 taattctggg ggtacagttc caatatcagc agcgcttatc ataggtacaa gtactttttt
     5461 cgctccggca tcttttgcta cttgtaaaca gttagctaaa ttatctacct taatcataga
     5521 accaccaatt gtaatatccc ctaaaataac taaacttggt aatacaggtt tatttaagga
     5581 aactgagcat aaagctatta tagttgataa cgataatgtt tttgtcatac caatgccatt
     5641 catatcttga acatgaacga caaaatcttt tgtcgtcaaa ctaatagact gactaacact
     5701 tccataatta gctcttaaat atcggtatgc cgaatccatc gcttctcttg ctccagcatc
     5761 tgaattgatc ccagtacgtt caaactttcc atttcccgaa gtcatttgcg tttccaattt
     5821 ataggtacct aattgcccag ttttgccata agataccgtg tacaaatgcc ctggcttccc
     5881 taacccttct ggaatcaaag ttcctccacc ttgttcagga acagaaacat actcttctcg
     5941 ctcttcttct aaatcaatgt atgaaaaatt gacatcataa aattccattc caccaatttt
     6001 ctttaattgt tcttttacac gacgacgcat ttcaagagca aatcttagta tttcttcgac
     6061 atcacttttt tcaaatctac catcaggata caacagtttc atcatcccag atacaatccg
     6121 acgaactgca atcgtatccc gctgatttaa ttgagaacca agtttgaaat atttatcaaa
     6181 actatcgcca aaactaactt ttcgaagctc acgcatgact tcagaaaaat aatctgtaat
     6241 gaagccatag tcatttgtaa aaaaatctgg tcgatattta ggaatttccc aacctggtaa
     6301 ataacaatgc atccgatcca aaaaggctga atccgtcccc ataacctccg gaaatggctc
     6361 aaataaattc gaagttttca atattacatc aacgctttga ttaatatttc ctacaaaagc
     6421 cattgatgct gaagcattct tttcttcttt gcctctagca aaagatcctg aagccatgta
     6481 atctttcatg atttgaacgc catcattatc cttaaattta atcccagcaa cttcgtcaaa
     6541 agcaacacag tcccacatac ctacaagtcc cattgtacga ctagccatat tatagaaaag
     6601 gtttgctact gttgtttgtc cacctgaaac aaggattgag tttggtgaaa tttctttata
     6661 tacatgcgat ttacctgtac cacgtggtcc tagttcacag agattaaaat tattttcaat
     6721 taatggaata agtcttgcta aatgaagcca ttttaccctt ttattaaatt gtgtaggttc
     6781 cataccaatg gaccgtagaa gtaagtcaat ccattcattt gtagtaaact ccttgcgagc
     6841 catcttgtaa gcctcaatgt ctaaaccagg catttggatt ggatcaacct tttttgcaat
     6901 aaaagggcta ttccaacgat cattctcatc ataccaatat tccatcgaaa caatacacca
     6961 gatcccccca cctaataatc gatcaaattt tttcggatac tcttcactaa tttgaacatt
     7021 ttttaatccc aggttagaga attctgcttc ataacaatct ttcttttcat ttaaatgtac
     7081 agttacttta tcaataactg tataaccacc caattcacga atttttgctt ttactttttc
     7141 tgcttcatca ggacgcacat agtttcgagc aagaatattt ttaacatttt ccacaccttt
     7201 ttcaaccaat gtttcatcca atgacgaagc atacatacca agtaaatatt ccaatacata
     7261 aacaggtaca ttcgcacctt ctttaatttt ctttgttaaa tcttttctga caatctttcc
     7321 gggaaagaat tcagataact ttaaaatttc ctgttgttcc actagaattc ctccatgtgt
     7381 tcagtcatta taatagcaat taaagtatat catacttaac cagtaggctt gataatacaa
     7441 aagaaaaagt atccacaaaa aattgtggat acctcgtttt tatatatcaa aatcaaatga
     7501 ttctgcaaaa ggattatcta taatgaattt tattcgctcg gtcaaatcat ttttttgatt
     7561 ttttatcact aaataataag tatcttcaac tgtatatcga cgatttacaa attcaaaaac
     7621 aaatttagta aaccgctgat ttgcatcttc tgtttttgaa tccgcaacat agatttcttc
     7681 atttgagata cgttcaccat attcatcttc aaaataaagc cagaaagatg cggggtcata
     7741 aacatcacta attgcatttt tttgatagaa ttctaacgtg gttgataatc ctgtaattct
     7801 gcggttgcta gtcattaatt caattgctac tggttccttt gcattgctac ctttggctac
     7861 cgtaatttct agaacaggga ctagcatttc ttgaatagat gaccctccat gaacgtagct
     7921 ttgtcccccg ccagctttct taaaaatact tgaagtaatc gggacacaaa tgtttctagt
     7981 gtcatcattt cctaaagaat ctcccaaaga gtaatttttc gtaccaatta tttcgtattc
     8041 ttcttcacta agaataaaac gacgttctac acgatctttt tcatttctag atggattttc
     8101 aatcttatct acttcttcaa cgtggcttct tttataaata aatccatgat cagccgtaac
     8161 tataaaacga tagatactgc catcattatg caagcgatta ataagtcggg aaatttcgct
     8221 tatcgctaaa tcacaggcct caaaaactcg attttcattc gagtgatcac ctgttctatc
     8281 cacttgatcg tgataaatat aaatgactga tttgccatta aatacttctc gtacctcagc
     8341 cttattcatc ttaaccacat catcataatt taaagctaca gcatttgcat ctcgttgctg
     8401 gagaatcgca tttcgattgg ataaaccatt cgtttttttc ccatcaatca gtacttctgt
     8461 tccttcattg taagttagtt gattgtttgg taataaggcg gctttaccga attcagtaac
     8521 tgaaggtaat acagaaagaa agctagccat gtttgttgaa tacttctttt ctctaactaa
     8581 cttacgttct aaattcatcc ctaactcata tcgaaaagca tctgaaataa taactacagt
     8641 tttaacagag cgtttcatta cagcttgatt ataaaatttt cgaatggatg gacgattctt
     8701 aaattcaaaa gtttgattcc aaatttgtcc tgattgatca agaaacttcg aataatttgc
     8761 ttcaactaat cgatacaatt ggccatattt ttgcttttct tctatatgat cactattcca
     8821 aataaatttc cgataatgcg tatcaaccca atacatttcc ttctcatatt gagttagcaa
     8881 attatccatg ttcatcggca agcacaatct gtttttatcc acaagataat aagcatgttt
     8941 caataattga tactcataaa tataatccga tgcgaaatgc attttttcgc gctgctttaa
     9001 actatcaaaa attgtttgtc ctgcaatttt cgttgtagtg tcagaagcct ttaatcgttc
     9061 tgcataaaac attaatattt ttgcatgaat ctcagaaaat acgtctgctt caattagatc
     9121 atcaatttct atactgtcta ataatccatc accatcaata gcctgatata cttttttaga
     9181 tagctgttca aagctttcca tgtaacgtgt atcattcata aactgatcca tgaaagtaat
     9241 aatgtttgct ttcaaatgta gtatttcaaa tcctttgaac ttcttcggca tctcttgatc
     9301 aatttgataa taaaaatagt tcaaataaag acacatcgtc agcttttcca atgttggctg
     9361 gctatccgta aagccaaatt tcatttgaat aaattcccaa aatttagctg ttaactgata
     9421 cttatcaagt tcccttaaag gattctccct actttctgta aagcttttta gaactacacg
     9481 cagtaaatca tttaatcgaa tacttttcga tttcaacaaa acagccatta tcattaattc
     9541 aatcgtttct gatttattaa ttgtcatatg cgtactaaat tcaccaaaac ttctcatgcg
     9601 atcctttgca ttaaagaaat tacgatactg tttaactgtc tgatgaaatc taagtggaat
     9661 agccaattca ttcatgataa tcgctaagcg gtctgcacta aattcctctg aataattcaa
     9721 tacactcaac aaatgattat cttcatcttt atcatcccaa cgaggaaatg gcgcatagat
     9781 taaatagtta gtttcgggat cttcatattc caataaacgc ttagttttaa attgtgcgtc
     9841 cttttctaat cgatagattt ttgcattttg aatgaccaat gagtcaattt cttcaagaaa
     9901 atcctgattt gaatcaaacc aaaaaatgat tttcctacat ccataattag gaaaacgttg
     9961 atttaatttt tcttcaattt gttgcgtatt tatatctgtc atcaatctca cctaatcgtt
    10021 ctctggattt gagtcaatat cttggacaca ttttagtaga gacggttaag ccattttttc
    10081 ttttgctaat tcttcaaatt cctgtggggt tagatagtga atcgagctat ggattcgatt
    10141 tcggttataa aatccttcaa tgtagctaaa tagcgctcga tttgcttctt caaaatctga
    10201 gtagctagtc gtgtagactt cttctttttt caatgaggca tggaaagatt cgattccagc
    10261 attatcataa ggtgttcctt ttctactata agaatgcctt attttgttgg ttttaagcca
    10321 ttgttccact tctcttgctg tgtattggct gcccaagtca gtgtgtagaa tcattccttc
    10381 tggaatgtga taatgtattt ttgctttatt caatgtttga atgacacaat ccacagtcat
    10441 tcgttttgaa aatgtataac taataatttt tttcgtgtgt aaatccatga ttgaagataa
    10501 gtaacaccaa ccatttttct tagtaggaat gtacgtaatg tcagctgccc atttctcaca
    10561 gatcgtttca gtagaaaagt cctgatttaa gagattctct tttgaaatga tcggtttatt
    10621 agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag
    10681 cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt
    10741 tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt
    10801 attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct
    10861 agggatttct aataaccgac ataacaaaga aacagtgtaa tccttacacc atttttcaat
    10921 aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt
    10981 tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc
    11041 agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat
    11101 aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc
    11161 atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt
    11221 aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc aagttatctt
    11281 tagaaccatt tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta
    11341 ctgtctagta gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct
    11401 gaaacagtct aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag
    11461 atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct
    11521 aagctactgg agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa
    11581 aagatggaag aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg
    11641 tccgttctta aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat
    11701 gtctttacca acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc
    11761 tgataaagat tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc
    11821 ttcatttaca agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat
    11881 tctttcatct atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat
    11941 tctttcatcc atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct
    12001 cttcacaccc aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct
    12061 tttactaata aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact
    12121 atcatactca aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata
    12181 atcctttgac tctacttgag accttcctat acggaataca gctttactgt ctgatatctc
    12241 tataccagca tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt
    12301 agtttcttgt gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact
    12361 gttagaagaa ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt
    12421 gtgcagccga tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca
    12481 gttcgtcctg atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg
    12541 ccgcccgata ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct
    12601 ccggcaactc ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc
    12661 ttaagtaaag catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag
    12721 ccaagatatc ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac
    12781 tttgggcatt tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat
    12841 tcattttttt gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat
    12901 acttttgaat ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa
    12961 tggcatgctt caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa
    13021 acttggcgtt ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag
    13081 cctcaaacag tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata
    13141 ctttttggat tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt
    13201 tccccatcaa aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta
    13261 acgttttaat tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat
    13321 tactttttga taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc
    13381 atcggttcgg tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac
    13441 aacatcaatc gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc
    13501 ttgaatagcc agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct
    13561 atcttcctca actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc
    13621 atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc
    13681 atatggcgtt ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa
    13741 catgatctgt tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc
    13801 gatggtggca gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat
    13861 tgcctgatcc tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa
    13921 caaataattg tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc
    13981 ccaattatgg gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa
    14041 attgattgct ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct
    14101 agcttgagct aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc
    14161 tccacttggt ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat
    14221 tgtcagccaa tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac
    14281 aaaataatcg atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa
    14341 atcgaaatgg tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac
    14401 ttgatgataa aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg
    14461 cgtccaagcg atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc
    14521 ttctttatag tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg
    14581 ctgatacttg tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg
    14641 gtaggcacta atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta
    14701 ttgcacaaaa atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc
    14761 cgcttgcttc actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat
    14821 catcgtagcc agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg
    14881 acacctgatc tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt
    14941 cgatccccac atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt
    15001 tagattctaa aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg
    15061 gatgaatggc tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag
    15121 aaataggaca cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg
    15181 tttgctctga ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg
    15241 caacaaaggc gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa
    15301 taaaggctgg taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca
    15361 agccagcata caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca
    15421 gcttcaaggt tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag
    15481 tggacacagc tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag
    15541 cacctgattg agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg
    15601 tactgctcaa ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca
    15661 aaaatggctg caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc
    15721 attcagcccc cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca
    15781 acgctaaagg ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg
    15841 aaggcgaaat tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa
    15901 tcggtaaaac tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg
    15961 catcgccagt agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa
    16021 tcgccaaaat tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca
    16081 taaaataaaa ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg
    16141 atagtacacc agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta
    16201 tagctggaat catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac
    16261 tttcttgatt gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt
    16321 ccttgaaggc tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca
    16381 ccacactaat tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt
    16441 tgagttcaaa acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc
    16501 caacctgttg caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa
    16561 gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt
    16621 ggtcttgcct gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata
    16681 gcacattatg atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta
    16741 tagggtaata taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta
    16801 aaataagtat atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa
    16861 attcccaaaa tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt
    16921 ctgctcttgg gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa
    16981 caatataggc aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa
    17041 tttcaatcat tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa
    17101 attaactagc aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat
    17161 tccaccaatt atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc
    17221 ttttatattg ttataacagt gaataacata aaaattcccg acaatcattg taatattttt
    17281 ttgtcacctg tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac
    17341 acctttggga gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa
    17401 cacctcagca tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt
    17461 tatgttgata gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt
    17521 ttcatgagta atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat
    17581 ctgtgttacc aatggtttcg atgacttctc taatccagat gtacctgtca tttttgaaaa
    17641 aatgatacct ataatagcca agacaacgat aactataatg ataaacttct tgttcatatt
    17701 tactttattc aactttttat ctcctatctc caaaataatt tgctcttatc cctcttatca
    17761 gtacattgac atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa
    17821 aatcgtaccc atttgttcat ctttatctcg atataagtag cccataataa cagcaacagc
    17881 accaaataca acaggaataa atgctaacgc tactaccata gaaatccaac caataatcaa
    17941 atacagcttc cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg
    18001 ctcagtactt ttctttactg cgtctgctcc agttgtctca tctgtttctt gtagtgctcc
    18061 gcaaaactgg caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat
    18121 gtcttctcct cctaaaaaca ttcaattgtt tattagttaa ctgtttactt tattaaatac
    18181 atcagaaaac agctcattca ttggtacact taatatctca gaaagcatct tcatttgata
    18241 gggtttagga atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat
    18301 ttttttgcta actccgtttg agaaagtcct ttttcttttc tgtatcggct gagattactt
    18361 acttttttca tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac
    18421 tcataaaata taaaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac
    18481 tgagatgggg gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga
    18541 aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca
    18601 gtagcaagat gggaaaattc tgtaacagag cctaacctcg atagcttaac cgctctttct
    18661 agagccctag gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa
    18721 gagtttttgg ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat
    18781 atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata
    18841 aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg
    18901 ataacctgtc tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc
    18961 tcttgttaca cttgtaaatt gataaagagg aactaattca actcctgata atatcaattc
    19021 ttttattact tcatctatcc ctgctccttt tgctaggtag actaaaaaac tactacttgc
    19081 tttcacattg tatagtctct taatatcctt agaagctcgt tcaagagtga atccttgttt
    19141 aacaaagtag cctctgtttt cttttaaagc attttccacg ctgttattcc caacattacg
    19201 tagacgttca caaacagtgt tagtaagttc tttctctgtc attagatatg ttccttgcca
    19261 cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctaag
    19321 ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt
    19381 aacccattca taagaaaaat attgatatgt aaaatgaaat ttttccagaa gtggaaggat
    19441 atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt
    19501 gcttctctta tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc
    19561 gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat
    19621 aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt aaaaactcat
    19681 ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa
    19741 aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct
    19801 tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc
    19861 ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa
    19921 tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact
    19981 gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat
    20041 actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag
    20101 taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga
    20161 ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat
    20221 caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca
    20281 atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc
    20341 ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc
    20401 atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat
    20461 tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt
    20521 ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga
    20581 taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta ctaccttccc
    20641 cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact
    20701 ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag
    20761 tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac
    20821 atcagttttt tctttttttg taactggctt ctctaataat ttttctttat ctacataaat
    20881 tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga
    20941 ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt
    21001 ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga
    21061 ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta
    21121 ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga gtgactccag
    21181 aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa
    21241 cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga
    21301 ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt
    21361 tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata
    21421 cctcgataaa aaagaggtgt caatatcttt gtaaaattta tttacaacac ccctttttcg
    21481 aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt
    21541 agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac
    21601 acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta
    21661 cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt
    21721 gggaaatatg ag
//
LOCUS       DACDOA010000044        21489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDOA010000044 DACDOA010000000
VERSION     DACDOA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.044"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..870
                     /locus_tag="IWI39_001481"
     CDS             169..870
                     /locus_tag="IWI39_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9208593.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="IWI39_001482"
     CDS             complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="IWI39_001482"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9208594.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="IWI39_001483"
     CDS             complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="IWI39_001483"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9208595.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="IWI39_001484"
     CDS             complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="IWI39_001484"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9208596.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3489..4313)
                     /gene="tsaB"
                     /locus_tag="IWI39_001485"
     CDS             complement(3489..4313)
                     /gene="tsaB"
                     /locus_tag="IWI39_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9208597.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4402..5382)
                     /locus_tag="IWI39_001486"
     CDS             complement(4402..5382)
                     /locus_tag="IWI39_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9208598.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5406..6890)
                     /gene="galT"
                     /locus_tag="IWI39_001487"
     CDS             complement(5406..6890)
                     /gene="galT"
                     /locus_tag="IWI39_001487"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9208599.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6902..7891)
                     /gene="galE"
                     /locus_tag="IWI39_001488"
     CDS             complement(6902..7891)
                     /gene="galE"
                     /locus_tag="IWI39_001488"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9208600.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8139..8306
                     /locus_tag="IWI39_001489"
     CDS             8139..8306
                     /locus_tag="IWI39_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9208601.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8368..10179)
                     /locus_tag="IWI39_001490"
     CDS             complement(8368..10179)
                     /locus_tag="IWI39_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9208602.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10176..10541)
                     /locus_tag="IWI39_001491"
     CDS             complement(10176..10541)
                     /locus_tag="IWI39_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9208603.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10704..11099)
                     /locus_tag="IWI39_001492"
     CDS             complement(10704..11099)
                     /locus_tag="IWI39_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9208604.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11117..12079)
                     /locus_tag="IWI39_001493"
     CDS             complement(11117..12079)
                     /locus_tag="IWI39_001493"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9208605.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12079..12291)
                     /locus_tag="IWI39_001494"
     CDS             complement(12079..12291)
                     /locus_tag="IWI39_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9208606.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12312..13010)
                     /locus_tag="IWI39_001495"
     CDS             complement(12312..13010)
                     /locus_tag="IWI39_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9208607.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13030..13572)
                     /locus_tag="IWI39_001496"
     CDS             complement(13030..13572)
                     /locus_tag="IWI39_001496"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9208608.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13704..14708)
                     /locus_tag="IWI39_001497"
     CDS             complement(13704..14708)
                     /locus_tag="IWI39_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9208609.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14705..15694)
                     /locus_tag="IWI39_001498"
     CDS             complement(14705..15694)
                     /locus_tag="IWI39_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9208610.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15691..16497)
                     /locus_tag="IWI39_001499"
     CDS             complement(15691..16497)
                     /locus_tag="IWI39_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208611.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16663..17619
                     /locus_tag="IWI39_001500"
     CDS             16663..17619
                     /locus_tag="IWI39_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9208612.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17696..18214)
                     /locus_tag="IWI39_001501"
     CDS             complement(17696..18214)
                     /locus_tag="IWI39_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9208613.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18302..18451)
                     /gene="rpmG"
                     /locus_tag="IWI39_001502"
     CDS             complement(18302..18451)
                     /gene="rpmG"
                     /locus_tag="IWI39_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9208614.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18679..19125
                     /locus_tag="IWI39_001503"
     CDS             18679..19125
                     /locus_tag="IWI39_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9208615.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19320..21215)
                     /gene="asnB"
                     /locus_tag="IWI39_001504"
     CDS             complement(19320..21215)
                     /gene="asnB"
                     /locus_tag="IWI39_001504"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9208616.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6469 a         4558 c         3701 g         6761 t
ORIGIN      
        1 attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat
       61 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
      121 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      181 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      241 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      301 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      361 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      421 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      481 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      541 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      601 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      661 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      721 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      781 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      841 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      901 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      961 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
     1021 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
     1081 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
     1141 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
     1201 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
     1261 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1321 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1381 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1441 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
     1501 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1561 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1621 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1681 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1741 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1801 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1861 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1921 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1981 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     2041 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2101 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2161 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2221 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2281 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2341 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2401 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2461 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2521 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2581 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2641 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2701 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2761 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     2821 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2881 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2941 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     3001 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     3061 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3121 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3181 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3241 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3301 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3361 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3421 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3481 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3541 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3601 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3661 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     3721 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3781 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     3841 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3901 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3961 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     4021 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4081 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4141 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4201 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4261 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     4321 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     4381 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     4441 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     4501 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     4561 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     4621 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     4681 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     4741 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     4801 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     4861 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4921 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4981 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     5041 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     5101 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     5161 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     5221 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     5281 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     5341 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     5401 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     5461 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     5521 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     5581 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     5641 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     5701 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     5761 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     5821 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     5881 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5941 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     6001 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     6061 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     6121 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     6181 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     6241 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     6301 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     6361 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     6421 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     6481 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     6541 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     6601 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     6661 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     6721 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     6781 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     6841 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6901 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6961 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     7021 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     7081 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     7141 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     7201 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     7261 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     7321 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     7381 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     7441 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     7501 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     7561 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     7621 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     7681 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     7741 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     7801 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     7861 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7921 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7981 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     8041 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     8101 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     8161 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     8221 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     8281 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     8341 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     8401 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     8461 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     8521 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     8581 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     8641 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     8701 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     8761 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     8821 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     8881 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8941 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     9001 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     9061 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     9121 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     9181 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     9241 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     9301 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     9361 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     9421 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     9481 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     9541 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     9601 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     9661 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     9721 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     9781 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     9841 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9901 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9961 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
    10021 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
    10081 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
    10141 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
    10201 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
    10261 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
    10321 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
    10381 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
    10441 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
    10501 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
    10561 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
    10621 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
    10681 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
    10741 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
    10801 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
    10861 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10921 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10981 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    11041 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    11101 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    11161 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    11221 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    11281 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    11341 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    11401 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    11461 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    11521 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    11581 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    11641 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    11701 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    11761 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    11821 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    11881 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11941 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    12001 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    12061 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    12121 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    12181 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    12241 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    12301 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    12361 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    12421 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    12481 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    12541 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    12601 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    12661 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    12721 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    12781 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    12841 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12901 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12961 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    13021 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    13081 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    13141 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    13201 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    13261 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    13321 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    13381 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    13441 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    13501 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    13561 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    13621 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    13681 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    13741 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    13801 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    13861 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13921 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13981 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    14041 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    14101 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    14161 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    14221 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    14281 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    14341 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    14401 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    14461 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    14521 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    14581 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    14641 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    14701 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    14761 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    14821 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    14881 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14941 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    15001 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    15061 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    15121 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    15181 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    15241 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    15301 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    15361 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    15421 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    15481 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    15541 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    15601 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    15661 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    15721 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    15781 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    15841 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15901 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15961 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    16021 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    16081 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    16141 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    16201 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    16261 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    16321 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    16381 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    16441 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    16501 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    16561 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    16621 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    16681 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    16741 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    16801 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    16861 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16921 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16981 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    17041 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    17101 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    17161 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    17221 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    17281 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    17341 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    17401 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    17461 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    17521 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    17581 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    17641 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    17701 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    17761 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    17821 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    17881 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17941 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    18001 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    18061 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    18121 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    18181 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    18241 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    18301 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    18361 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    18421 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    18481 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    18541 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    18601 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    18661 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    18721 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    18781 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    18841 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18901 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18961 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    19021 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    19081 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    19141 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    19201 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    19261 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    19321 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    19381 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    19441 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    19501 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    19561 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    19621 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    19681 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    19741 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    19801 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    19861 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19921 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19981 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    20041 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    20101 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    20161 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    20221 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    20281 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    20341 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    20401 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    20461 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    20521 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    20581 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    20641 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    20701 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    20761 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    20821 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    20881 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20941 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    21001 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    21061 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    21121 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    21181 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    21241 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    21301 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    21361 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    21421 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    21481 gatcgtgtc
//
LOCUS       DACDOA010000045        21306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDOA010000045 DACDOA010000000
VERSION     DACDOA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.045"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..575)
                     /locus_tag="IWI39_001505"
     CDS             complement(<1..575)
                     /locus_tag="IWI39_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208617.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKV"
     gene            complement(870..1205)
                     /locus_tag="IWI39_001506"
     CDS             complement(870..1205)
                     /locus_tag="IWI39_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9208618.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1192..1476)
                     /locus_tag="IWI39_001507"
     CDS             complement(1192..1476)
                     /locus_tag="IWI39_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9208619.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1532..3055)
                     /locus_tag="IWI39_001508"
     CDS             complement(1532..3055)
                     /locus_tag="IWI39_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9208620.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3048..4223)
                     /locus_tag="IWI39_001509"
     CDS             complement(3048..4223)
                     /locus_tag="IWI39_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9208621.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4227..4412)
                     /locus_tag="IWI39_001510"
     CDS             complement(4227..4412)
                     /locus_tag="IWI39_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208622.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4378..6072)
                     /locus_tag="IWI39_001511"
     CDS             complement(4378..6072)
                     /locus_tag="IWI39_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9208623.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPVLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6069..6542)
                     /locus_tag="IWI39_001512"
     CDS             complement(6069..6542)
                     /locus_tag="IWI39_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9208624.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6611..6766)
                     /locus_tag="IWI39_001513"
     CDS             complement(6611..6766)
                     /locus_tag="IWI39_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9208625.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6900..7280)
                     /locus_tag="IWI39_001514"
     CDS             complement(6900..7280)
                     /locus_tag="IWI39_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9208626.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7284..7499)
                     /locus_tag="IWI39_001515"
     CDS             complement(7284..7499)
                     /locus_tag="IWI39_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208627.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7502..7909)
                     /locus_tag="IWI39_001516"
     CDS             complement(7502..7909)
                     /locus_tag="IWI39_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9208628.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8175..9650)
                     /locus_tag="IWI39_001517"
     CDS             complement(8175..9650)
                     /locus_tag="IWI39_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9208629.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9640..10488)
                     /locus_tag="IWI39_001518"
     CDS             complement(9640..10488)
                     /locus_tag="IWI39_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9208630.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10525..10887)
                     /locus_tag="IWI39_001519"
     CDS             complement(10525..10887)
                     /locus_tag="IWI39_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208631.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11187..11811)
                     /locus_tag="IWI39_001520"
                     /pseudo
     CDS             complement(11187..11811)
                     /locus_tag="IWI39_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11808..12119)
                     /locus_tag="IWI39_001521"
     CDS             complement(11808..12119)
                     /locus_tag="IWI39_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208632.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12163..12456)
                     /locus_tag="IWI39_001522"
     CDS             complement(12163..12456)
                     /locus_tag="IWI39_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208633.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12650..13297
                     /locus_tag="IWI39_001523"
     CDS             12650..13297
                     /locus_tag="IWI39_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208634.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13358..14503
                     /locus_tag="IWI39_001524"
     CDS             13358..14503
                     /locus_tag="IWI39_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9208635.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14584..14889)
                     /locus_tag="IWI39_001525"
     CDS             complement(14584..14889)
                     /locus_tag="IWI39_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9208636.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14914..15345)
                     /gene="ruvX"
                     /locus_tag="IWI39_001526"
     CDS             complement(14914..15345)
                     /gene="ruvX"
                     /locus_tag="IWI39_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9208637.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15342..15608)
                     /locus_tag="IWI39_001527"
     CDS             complement(15342..15608)
                     /locus_tag="IWI39_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9208638.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(15863..16588)
                     /locus_tag="IWI39_001528"
     CDS             complement(15863..16588)
                     /locus_tag="IWI39_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9208639.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(16949..17019)
                     /locus_tag="IWI39_001529"
     tRNA            complement(16949..17019)
                     /locus_tag="IWI39_001529"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16985..16987),aa:Gly,seq:ccc)
     gene            17136..17888
                     /locus_tag="IWI39_001530"
     CDS             17136..17888
                     /locus_tag="IWI39_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9208640.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17904..18383
                     /locus_tag="IWI39_001531"
     CDS             17904..18383
                     /locus_tag="IWI39_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9208641.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18634..20214)
                     /locus_tag="IWI39_001532"
     CDS             complement(18634..20214)
                     /locus_tag="IWI39_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9208642.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20207..20950)
                     /locus_tag="IWI39_001533"
     CDS             complement(20207..20950)
                     /locus_tag="IWI39_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208643.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21126..>21306)
                     /locus_tag="IWI39_001534"
     CDS             complement(21126..>21306)
                     /locus_tag="IWI39_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP9208644.1"
                     /translation="YEYLCKDLKAYIINTENITSISRVNEIVTFANGEQLEMGKRSID
                     KLKNFMQEKIENESF"
BASE COUNT         6222 a         4341 c         3467 g         7276 t
ORIGIN      
        1 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
       61 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      121 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      181 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      241 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      301 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      361 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      421 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      481 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      541 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
      601 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
      661 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
      721 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
      781 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
      841 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
      901 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
      961 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1021 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1081 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1141 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1201 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1261 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1321 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1381 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1441 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1501 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1561 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     1621 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     1681 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     1741 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     1801 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     1861 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     1921 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     1981 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2041 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2101 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2161 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2221 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2281 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2341 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2401 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2461 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2521 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     2581 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     2641 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     2701 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     2761 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     2821 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     2881 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     2941 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3001 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3061 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3121 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3181 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3241 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3301 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3361 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3421 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3481 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3541 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     3601 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     3661 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     3721 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     3781 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     3841 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     3901 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     3961 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4021 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4081 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4141 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4201 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4261 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4321 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4381 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4441 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4501 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4561 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     4621 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacactgg
     4681 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     4741 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     4801 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     4861 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     4921 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     4981 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5041 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5101 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5161 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5221 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5281 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5341 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5401 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5461 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5521 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     5581 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     5641 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     5701 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     5761 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     5821 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     5881 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     5941 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6001 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6061 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6121 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6181 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6241 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6301 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6361 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6421 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6481 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6541 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     6601 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     6661 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     6721 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     6781 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     6841 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     6901 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     6961 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7021 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7081 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7141 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7201 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7261 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7321 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7381 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7441 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7501 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7561 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     7621 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     7681 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     7741 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     7801 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     7861 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     7921 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     7981 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8041 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8101 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8161 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8221 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8281 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8341 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8401 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8461 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8521 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     8581 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     8641 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     8701 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     8761 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     8821 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     8881 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     8941 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9001 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9061 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9121 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9181 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9241 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9301 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9361 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9421 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9481 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9541 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
     9601 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
     9661 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
     9721 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
     9781 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
     9841 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
     9901 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
     9961 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10021 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10081 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10141 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10201 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10261 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10321 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10381 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10441 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10501 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10561 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    10621 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    10681 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    10741 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    10801 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    10861 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    10921 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    10981 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11041 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11101 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11161 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11221 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11281 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11341 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11401 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11461 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11521 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    11581 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    11641 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    11701 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    11761 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    11821 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    11881 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    11941 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12001 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12061 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12121 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12181 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12241 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12301 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12361 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12421 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12481 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12541 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    12601 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    12661 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    12721 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    12781 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    12841 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    12901 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    12961 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13021 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13081 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13141 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13201 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13261 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13321 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13381 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13441 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13501 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13561 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    13621 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    13681 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    13741 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    13801 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    13861 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    13921 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    13981 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14041 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14101 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14161 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14221 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14281 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14341 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14401 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14461 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14521 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    14581 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    14641 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    14701 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    14761 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    14821 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    14881 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    14941 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15001 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15061 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15121 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15181 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15241 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15301 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15361 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15421 aaatttcgag catcacgata acgaggaatg taggacgggt ctccagaaag caagtagccg
    15481 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15541 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    15601 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    15661 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    15721 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    15781 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    15841 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    15901 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    15961 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16021 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16081 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16141 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16201 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16261 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16321 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16381 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16441 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16501 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16561 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    16621 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    16681 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    16741 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    16801 tacattgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    16861 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    16921 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    16981 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17041 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17101 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17161 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17221 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17281 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17341 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17401 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17461 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17521 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    17581 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    17641 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    17701 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    17761 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    17821 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    17881 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    17941 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18001 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18061 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18121 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18181 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18241 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18301 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18361 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18421 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18481 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18541 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    18601 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    18661 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    18721 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    18781 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    18841 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    18901 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    18961 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19021 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19081 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19141 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19201 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19261 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19321 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19381 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19441 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19501 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19561 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    19621 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    19681 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    19741 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    19801 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    19861 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    19921 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    19981 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20041 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20101 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20161 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20221 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20281 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20341 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20401 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20461 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20521 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    20581 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    20641 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    20701 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    20761 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    20821 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    20881 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    20941 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21001 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21061 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21121 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21181 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21241 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21301 tcatat
//
LOCUS       DACDOA010000046        21192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDOA010000046 DACDOA010000000
VERSION     DACDOA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.046"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2065)
                     /locus_tag="IWI39_001535"
     CDS             complement(<1..2065)
                     /locus_tag="IWI39_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9208645.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSV"
     gene            complement(2082..2369)
                     /locus_tag="IWI39_001536"
     CDS             complement(2082..2369)
                     /locus_tag="IWI39_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208646.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(2423..2791)
                     /locus_tag="IWI39_001537"
     CDS             complement(2423..2791)
                     /locus_tag="IWI39_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208647.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(2837..3349)
                     /locus_tag="IWI39_001538"
     CDS             complement(2837..3349)
                     /locus_tag="IWI39_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP9208648.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(3360..3752)
                     /locus_tag="IWI39_001539"
     CDS             complement(3360..3752)
                     /locus_tag="IWI39_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP9208649.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(3752..4090)
                     /locus_tag="IWI39_001540"
     CDS             complement(3752..4090)
                     /locus_tag="IWI39_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9208650.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(4074..4400)
                     /locus_tag="IWI39_001541"
     CDS             complement(4074..4400)
                     /locus_tag="IWI39_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208651.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(4397..4726)
                     /locus_tag="IWI39_001542"
     CDS             complement(4397..4726)
                     /locus_tag="IWI39_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP9208652.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(4738..5001)
                     /locus_tag="IWI39_001543"
     CDS             complement(4738..5001)
                     /locus_tag="IWI39_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208653.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(5019..5942)
                     /locus_tag="IWI39_001544"
     CDS             complement(5019..5942)
                     /locus_tag="IWI39_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9208654.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(5955..6557)
                     /locus_tag="IWI39_001545"
     CDS             complement(5955..6557)
                     /locus_tag="IWI39_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9208655.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(6684..7610)
                     /locus_tag="IWI39_001546"
     CDS             complement(6684..7610)
                     /locus_tag="IWI39_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9208656.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(7615..9114)
                     /locus_tag="IWI39_001547"
     CDS             complement(7615..9114)
                     /locus_tag="IWI39_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9208657.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(9127..10371)
                     /locus_tag="IWI39_001548"
     CDS             complement(9127..10371)
                     /locus_tag="IWI39_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP9208658.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(10358..10891)
                     /locus_tag="IWI39_001549"
     CDS             complement(10358..10891)
                     /locus_tag="IWI39_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP9208659.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(10918..11142)
                     /locus_tag="IWI39_001550"
     CDS             complement(10918..11142)
                     /locus_tag="IWI39_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208660.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(11149..11361)
                     /locus_tag="IWI39_001551"
     CDS             complement(11149..11361)
                     /locus_tag="IWI39_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208661.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            11723..11902
                     /locus_tag="IWI39_001552"
     CDS             11723..11902
                     /locus_tag="IWI39_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP9208662.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            complement(11895..12236)
                     /locus_tag="IWI39_001553"
     CDS             complement(11895..12236)
                     /locus_tag="IWI39_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208663.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(12535..13002)
                     /locus_tag="IWI39_001554"
     CDS             complement(12535..13002)
                     /locus_tag="IWI39_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9208664.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(13594..13881)
                     /locus_tag="IWI39_001555"
     CDS             complement(13594..13881)
                     /locus_tag="IWI39_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208665.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(13878..14087)
                     /locus_tag="IWI39_001556"
     CDS             complement(13878..14087)
                     /locus_tag="IWI39_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208666.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(14084..14281)
                     /locus_tag="IWI39_001557"
     CDS             complement(14084..14281)
                     /locus_tag="IWI39_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208667.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(14278..14604)
                     /locus_tag="IWI39_001558"
     CDS             complement(14278..14604)
                     /locus_tag="IWI39_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208668.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(14601..14882)
                     /locus_tag="IWI39_001559"
     CDS             complement(14601..14882)
                     /locus_tag="IWI39_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208669.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(14895..15113)
                     /locus_tag="IWI39_001560"
     CDS             complement(14895..15113)
                     /locus_tag="IWI39_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP9208670.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(15110..15421)
                     /locus_tag="IWI39_001561"
     CDS             complement(15110..15421)
                     /locus_tag="IWI39_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208671.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(15598..15912)
                     /locus_tag="IWI39_001562"
     CDS             complement(15598..15912)
                     /locus_tag="IWI39_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9208672.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(15912..16217)
                     /locus_tag="IWI39_001563"
     CDS             complement(15912..16217)
                     /locus_tag="IWI39_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208673.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(16214..16375)
                     /locus_tag="IWI39_001564"
     CDS             complement(16214..16375)
                     /locus_tag="IWI39_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9208674.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(16390..16749)
                     /locus_tag="IWI39_001565"
     CDS             complement(16390..16749)
                     /locus_tag="IWI39_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9208675.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(16746..17597)
                     /locus_tag="IWI39_001566"
     CDS             complement(16746..17597)
                     /locus_tag="IWI39_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9208676.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(17614..18447)
                     /locus_tag="IWI39_001567"
     CDS             complement(17614..18447)
                     /locus_tag="IWI39_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP9208677.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(18454..19140)
                     /locus_tag="IWI39_001568"
     CDS             complement(18454..19140)
                     /locus_tag="IWI39_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208678.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(19146..19892)
                     /locus_tag="IWI39_001569"
     CDS             complement(19146..19892)
                     /locus_tag="IWI39_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9208679.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(19885..20226)
                     /locus_tag="IWI39_001570"
     CDS             complement(19885..20226)
                     /locus_tag="IWI39_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208680.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(20405..21112)
                     /locus_tag="IWI39_001571"
     CDS             complement(20405..21112)
                     /locus_tag="IWI39_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9208681.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
BASE COUNT         5701 a         4516 c         3254 g         7721 t
ORIGIN      
        1 ttacagatgt ccaaatattt accactgatt ggacaagtgt gttccagatt agatctaaat
       61 cctctttcat ttgttcgaag tcaccagtaa ttaaatctat aataaacagt agtggcgcag
      121 ctattaaaga cttaatgatt tcccaagcat ttactattat gtttttaacc tcagcaaata
      181 tacttgttat tgctttcact atattagaga acacatctga aaagctactt acaaatggac
      241 caatatattt tagtataaaa tcaaaaccag atttgatttt acttgtaatt gttttccatg
      301 cagtagatat agtattttta aacccttccc atttttcacc agcacttgaa actaaatcat
      361 caataactga tatgacacct tcttttaaac tattccaaat ctctgaagca gagtttgcaa
      421 tacttgacca taaatttgaa aagaactctt ttgttccagt ccatttgttt ttaatccaat
      481 ctgccgcttt cccaggggct tcttgaactg tagtccaaac attgtctgcg ccttctttaa
      541 tggacttcca taaattgtta aaccattctc ctgtagattt ccatgcattc tgaatccatt
      601 ctactgccga gcttacagca gacttgattc cctcccataa gccaatccaa aagtttctaa
      661 aatcttcact cgtattccaa agatagatga aacctacaac gagtagtgct accgcagcta
      721 taaccagaaa aaccggattt gtaagaagac ccattgcttt acttaatcca ctaaacaatt
      781 ttgaactttc acttaaagct ttaaaagcta ctttcaccct attgatccat ataattactg
      841 aaccaatcat aaaaattaac ggtcctatag cagcgacaat tgctcctatg gccactacta
      901 atttttgagt tgattctgga gcacttacaa atttttctac taagccggat atggcatctg
      961 ctactttttt gatggatggt gctagaatct tttgaattac aatagctgct gactcaaaag
     1021 ctccaaacat ttgctcgatg gaagaattca tattatcttg catggtccga gccatatcgt
     1081 cagctgcacc atcagaatct ttcagagatt ttgttaattt gcccaatgaa tcaggtcctt
     1141 tatcaatcaa agccatcatc cctgataatg attcttgccc atatagtgtt actaaagcat
     1201 tttgttgttg ttcaggcgtc aggccttcaa aagctttttt aagtaattct acttgagttt
     1261 ttaaaggttt cattttaccg tcagcatcat aaaacgaaac acctaaatta tccattgtat
     1321 cttgcatagc ctttgttggc cttgctaacc tagacaatgc tcctcgcaac gttgtacctg
     1381 cttgagaacc cttaatgcct gcgtcactca taataccaat agctgctgca gtttcttcca
     1441 aagaaatccc cattgaatta gctacaggag caacatactt caatgcttct cccatgtctc
     1501 caacttcagc attggtatcc gcagcagcac gagcaaatac atcagcgaca tgtcctgctt
     1561 cacttgcttc taaaccaaat cctctcaaag cagtagcagt attttcagaa gctagagcca
     1621 catcccctcc agatacagct gctaagtcta aaagacccgg cattgctttc atgatttctt
     1681 gtgcgctaaa tccagcagaa gctaagtttt ccattccagc agctgattct tttgcgctaa
     1741 aagcagtttt tgctcctaga tcaatcgctt gctgtttcat ctgttcgaat gtgtcgccgg
     1801 ttgctcctga tatagctttt acacgactca tttgtgcttc gaagtcgcca ccaactttag
     1861 cagctgctac gcctactcct ataagaggag tggtaatata ctttgtcatt gcggcaccag
     1921 taccttgcat cactttacca acagcggttg tcatactatt tgaattcttt tcaaaagttt
     1981 taacagcatc ttgtgcatct ttaaaagtct ttacaaatcc actatctgtg gcttttaata
     2041 aggcttcaac agaaaattgt tccatgattc tcctcctttc ctcaagagtt agctttagtt
     2101 agtaagcttt ggaatttttt atcttgtttt gaaagttcgg aaactcccat gattgaatct
     2161 tcgatttttt gataattgaa gaattcttca aaggatcgat atacaggaac tgtctttttg
     2221 cctacttttt tctccgcttg gacttgctga tttgcccacg ctaattcgtg aatcaacttt
     2281 tctttgtcaa gccaagataa ctgggctgca gtcatacgaa tgttgtattc atataacgtc
     2341 attctttcga tatctgagat attggtcatt cccaaatatc gaaaagaatt gataagaatt
     2401 tgttcgtatg ccagtgcaga atctattcca cttgttgttt ttccgcttct ttcaatttct
     2461 gattcaggtt tcggaccgct aactttcccg cgttcgactc cgccaattct tttaggactt
     2521 catcaaacaa tttttcaatg tctttaactt catcgatgta atcatccaat tcatctaatg
     2581 taatagattc atcttcagtt ctatttgcta tttccagaac tcgtgataac gtgttaacgt
     2641 tataagaacg tagctctggt aagacttttg cggagagtcc cattccaaat tccatatttc
     2701 catcgacgaa aggcatcact ttatctaatt cacgtacaaa tttagtgcca aatttaaacg
     2761 aatattcttt acctttaatt tttaatttca atgtttttca tcctcctaaa ataaaaaaga
     2821 gagcatctaa gccctcttat gctcctgtcg aagttgcttt cacggtatct ttgaatgcat
     2881 attgaacaac atcggcttga tcttctgtca aggttgcata accatcttga ccaacaccat
     2941 ttactgcaaa tgataaactt aattcaacgt tatcctctgc agcagccgat ggagtaaatt
     3001 cagacacata tgcttggtaa taagtagctt tgtacttatt tgcattatca tctgttccct
     3061 gttctgcttt attaatttcc caaatttcaa tgatatcccc atttaataaa gcttgtttca
     3121 tttcatctac atgagaatct cctttagcaa ctattgaagt agccgaaaaa tcatattcaa
     3181 ccgggcttaa actttgaacg tttccatctt ttgtcactgt agagtctgaa tctcttgata
     3241 aaccattttc gtgttctgtt tgaaatgcca ttttccaagc ggcttcctga gtttcttttt
     3301 tcaataagcg ataaagcaaa atgacatcaa taccttttaa tgcttccatg ttcttcctcc
     3361 tatctaattc taaattcaag tgtgacaacc gcccgtttta gcggtgtatt cgttgttgtg
     3421 tcgtccatca cttgaattcc acttgcttga taatttaaag cccaataata gccttctgtg
     3481 gcttctatca atctagcttc attaaaaaga gcagatgcca tatctgacaa ctgctttcgt
     3541 ttcttctgta atccccaaac ggataaaacc acaatcacag accctttaat gtcagtttta
     3601 tttacttcat ggattgtctg agtgttctca aattccacaa aaggataacc aacatcctct
     3661 aaagttttgt aatcgtatgt tttatatcca agtttgtttt gggatatttt aaaaagttca
     3721 tcaaaaattg attgatctct tgtcttaatc atcatttcac caaggctttc atttcagcca
     3781 taaatttgac tttttggtaa ttaaacgctg gtctaacata aggctgtgcc gacataaatc
     3841 gagtgccata ttctacataa ggtgcataat ctgctgtcgg tcctacaata ccagttaaac
     3901 cagcttctaa aagattcatg tttattgatc ttcgtaagta acctgtatcc actggcgcac
     3961 ctttttgcat tcgttcagtc atttcagcag tattactttt cacgactttt tgaacgtcat
     4021 taagcgttgc tgcttttttc agatgtcgca tcagctgatc gattccttta tattcaagtt
     4081 gtgccttcat caagaaccac ctcttgcaca attaaactat ttctatatgc tggatttcta
     4141 gctgtttttt gttgccaagt ctttccttca atctcgatat agtcaaatgt agggatagaa
     4201 aaaagaggct gcgtcctaat gaccttcgcc ccttcttcca cactaccaaa aatagtcaca
     4261 cttctatcag tgccaatatc tgtcacgttt gcctctgttc ttgttctttc tggtttgcct
     4321 tcaacccact caccgagatc tggatcatat ttagagtcag atgaacgttt aacaaatata
     4381 atttcatctg taaatctcat acaaatttaa acctccctcg ctttggcttg tacaactctt
     4441 cctgatcttt acgcttaaat tcatcaatct cattttgata ctctgaaaaa tccgagtcag
     4501 gaaaagccat agataaacct tcttgagaat atgactgcat accttcttga ccaattctat
     4561 tgaatctttt caacgatact tcatatacaa ctgtttcaaa ttctttagga acttcttgcg
     4621 tatttaacaa ggttttcata cgctcattcg ttcttcgctc aataacttca agcttttcat
     4681 ctattgttcc tttaagaagt tttttaatat catctgcaat catcatattt ttacttccta
     4741 actagcaggt tgacctgtca cattgattga agtagtgaat tctccagaag taaatgtgaa
     4801 tgttgctgac ccttctgctg caatcgttcc atcaaaacca ccattttcat ttttggtcac
     4861 tgttgcgata gctccatcac ttgaagttgc tgtagtagct gcaacaacag cagctgcatc
     4921 gctagcatct gcaggcacag ctgaaatagt aaatgtttta gtatcgccta ctttaccggt
     4981 ccatgtcttt tgatttggca caataccggt agcaggcgtt acgcttttgg ggaaatcttc
     5041 ccaaatgctt catcttttac aatcataaat ccaacatcca ttgttgcacg caaagcaatc
     5101 agttcttgct caaacaagtt aactggggtt ccatcttcat tagttaaagt agacaattgg
     5161 gcttcttcag aaatcttaaa tgaaatatta tatgggatcc cataaaacat gtaattaaag
     5221 tctccagcgt aaagtgttcc tttatctaaa gacttaaggt ctactactgg taatccgtca
     5281 attgtattag tagagcgatc ataaataaac tcaacatttg acccgactgt ttgagctgca
     5341 gaacgtaatt ctgtacgatt ttttcggttt gaaataaacg cattaggttc gaattcattt
     5401 tctgctaact tgtcttctaa ggctaggata ttatcataag tcaatccgcc ttcaaccaca
     5461 ttccccgcac taataactga tccgtctagt gactgaggaa atgggttttc tttatttaat
     5521 aaggcagctg catcaaattt tttatagaaa gcttcagcaa tttttggctg catctcctca
     5581 aagaaatctg ataatttata atttaaatat tcacgagaaa ccggaagaat gacaccgagt
     5641 ttttttgcag tcatcgtagc ttgcatccat ttaggtttag acgttttaat tttttcacct
     5701 tcacccaccc agtatgcgcc tggtccttct gcaaagtatt caaatttctt ttctttgtca
     5761 gtcatttctt cgtattttgc taactgcatg atcttagaat tttccataac ttcactcaaa
     5821 atgagcgtat tatatttatc aggaattttt ccctctttcg tttcatatac caagacatta
     5881 tctggatccc atgtttgagc aaacatttgc aagttcattg gtaaaagttg tttctttttc
     5941 attaagtttt cctcctattt gataattcga tttttagcag ctagtttagc cactgtttct
     6001 ttagtatttt tcgatgctgt aaattgtcca ccttcatttg gtggtgtttg tcttgcgttt
     6061 tctctcttaa tcaaagaagc aaagttagtg atgactgcta cagcttgttt tgtggcatct
     6121 gcatcatcag aaacaatcag accaagtaaa tcatcatcgt gtggtaaatt tgcatctgtc
     6181 agcattttag aagcttcttt cgtcatttca gatagtgcct gtccacgctt taattcagcg
     6241 atttcagctt ctttttgctc caactcatgc tgtagttttt cttccgcatt catttttgcc
     6301 agctttttag cttcttcttt ttttgctgct agttcttttt cccacgctgc ttttgcttta
     6361 ttcgtctcag cagcgatcat tttcgctact tcatcacgag aaaatgtttt gccagtattg
     6421 ttttcttctt ttgcttcggg cggtgtctct tgtgagccag ctggtaggtt tccttgttgt
     6481 ccctcatcac cagatccacc atctcctggt tcagaaaaaa attgtaagtt cattggcata
     6541 aataaacgtt ttttcatgat taatcctcca cggttacgcc gctacccgat aaatttgacc
     6601 agttacgccg gtcagccgaa aatagctttc tctttaacgc ctgtaagctg taagaaggca
     6661 caataaaaag ccgttaattt gtgttaacgg cttgatattc gttctatata aggtgctgta
     6721 ctgcatcgac aaaatggatg catattagga gcgttacttc ctggctgcat atcggcaaca
     6781 tcaaaaactt gattatttaa cggtatacat agtttgcacg ccgttggttc tgctatatag
     6841 atgtactggg taatgcctgc atctctataa cttcgttctt gaatccctac ctgaactcta
     6901 gtcgtttcag tcaccatcaa acgttgagtg ttgaacttag tgttttctcg tccttcagct
     6961 gttaaatatt ctgccaattt agatgcaagt tgcttgggat ttttccccat cgttatactt
     7021 ctgactaaca acctatctaa ttctgatttc aattcagatt gatacatcca taagcgatca
     7081 ctaaaagaca cgtcatcact caaaaaggag ctgtttatta ctagttctat cagctttgca
     7141 tatccacttg aagcaatagt catttctaaa ataccggctt gtctcttcaa ttcagctaaa
     7201 ccagcttttg ttaattcatt cgaaaagtac ttatccaatt cattaaacag tgaaatcaat
     7261 tcaagcccga tattagcttt taagagctct aatctattta cacgcatcgt aagattgtat
     7321 agctttaatt cttggtttgc tgtaggagaa aaatctttct ctttgacata cttctttgct
     7381 ttgcgagcga atgctttgac atccatttca ctagcacgct tcatcgcttc gctacgagtg
     7441 attttctggc cattggagaa actgtcccac tgcgcgtcta tttctttttg tatcgcatct
     7501 tgtgcgtatt gcatgcgact tttaatttct gccatgcgct ttttatcatc tttaatttgt
     7561 tgcttttgcc attccttttc ccgtttgatc caatattctt gggagttcat ttaatcaccc
     7621 ccctgtttca tcttttttgt tgttaattac ttcttcacca tctgaatcaa aaatgccaat
     7681 ctgcttttgc gtttctttat ttactcgttt caactctgcc tgtacatctg gaacaaaaga
     7741 agcgagtcct aagatcgtct cttgactgag ttcagctcca gcatcaacca aagatttcaa
     7801 ctcttccaga atggctttag gtagattagg cgtaaatatt acacgtaagc ctttcaaatc
     7861 ggagttatcc atttcagaaa tacttgattt taggctaaat aaaagacgat aacgccgcat
     7921 aaggcctttt ttgaatagcc tttgctttgt tgccgtcatt tgttcaaatc caaataattt
     7981 atatttcatt gcttctcccg attgcactcc ggaaaaattg tcatcagtaa gatcaggaac
     8041 catggatatt tcgtgaatat ccttgcgtac tctgtatttg tatgcttcta caccgttcac
     8101 atcatattgt ttgtaaatat atcctgcagt cacacttgtt ttattaccgt tcacatcagt
     8161 tccagactca agcaaaagca tattcgcttc tttctgcttg atggcgtcct ctgtggatag
     8221 tcctgctgct tcaatatcac cactaataac tagaagagca tcgtttagat cagtcatata
     8281 attggcggta tcagactgcc ctgcatcgta tagatcaatc aaagatagta catcttcata
     8341 caagcccatc cgaaaacgat taggagaata ctctgtaata ggtacctctt tatattcatg
     8401 cggttcatcc tggggattct ttaactcaat tgctgttagt gtcgtctcat cataagtgat
     8461 acttttttct tttgtgtata cgattggttg aatgtactgt ttatcagcat ccttgttgaa
     8521 tcttgtctta ggataccgta cagccaaaat aggctctcgt tttactgtag tatcatatac
     8581 aacaaacgtt tcaaatacat tagccaaatc aacataatct gtatcatctg aatctcgata
     8641 gataatctca taggctctcc catacttatc catatcaagc cagagttcag catttaaccc
     8701 atctatgtca ttattagtat taaactcttc gatttctttt tgttgatttg tgtcctcgat
     8761 ttgcactttt ataggattgc ctgtgttgta cccaacatca aacgtacaaa gaacttttcc
     8821 aaagttatgt gctgatcggt gatccgcttt ttctttttct ctgcgcctac ggttatccat
     8881 gatatttgta tttctagctt tataataatc atctaataca cttagccttt ttacctgata
     8941 ttcatgatga tgttttatca ttgctgctaa agtatctaaa tcgttaagta aatcttctgc
     9001 tgagctaaat ctatagtgaa gatttgagtc tacgctaaac tttacataat ttgtgttcac
     9061 gtcattagaa taatgtatat cagctccgta ttcaaactcg tttaccttat ccatttttca
     9121 cactccttaa aacatgcgtt taatacgttt aatcttttcg ttgacattaa cttttggttt
     9181 cttaagaagc tcatgagtat atatcgcata tcgaacggag tctagcacat catcatattc
     9241 ttttattggc tcgccttttt tcttgtccca aatgtattga taaatttcat cccgaaattt
     9301 catcactcta tcttgacaga taaacaattt atcttgttta aatctcttgg cgacttcctc
     9361 aactccagat aaccgtgctt tatgagcatt tttagcatta atcttttctc ttacaaacct
     9421 agccacatgc tccggtctcg cagagtcaca ataaaaaggt actcgtaagc cgtaacgctc
     9481 ttgaatgcct tttgccacat ctacccaata atcaatctct tcaaattgag tagcatgttc
     9541 ttcgattaaa taagctgttc cgtcatccgt ttcacctata actacgattg aaccccagtg
     9601 ttcatatccc cagtcaacac cacaataaaa gttgctcaaa ggtggtaagt cttttgactg
     9661 gatgtagtgc ttgctagcat caaaatcacg ataaacgaca ccttctgcag aaacccataa
     9721 acctttgata tcacgatcat aaaacatgcc gcttggtgtt gattctttaa tattgttacg
     9781 atatcgttca gataaaaatg tattatcatc taattcaaag tggaacgatt gaatattttt
     9841 gcttgaattg tcgatatact ctttctttag ccagtgttcc gggttgtcag ggtttgtatc
     9901 agctagtatt ctcgctcctg ttcctgaaca acgtgaaacg atttcagcaa atacttcttg
     9961 tctagctagc gatgcctcat tgatataagc gccatatgct gtcataccac gaattgcgcc
    10021 aacaccgcca atgtttcctg tataagcttg tacgacctta acgccaaata gcttaaagtt
    10081 tccatgttta tcgaatttag gttctatgct gtacatgttg tatagttcct gtaagatgtt
    10141 cttttggatt gttgcacttg aaactcctgc taggatatac attggttcct taatgtcttc
    10201 ttcatcggca atctttcgca cacgtcgcaa ttcaaacaag aacaaatcat tgtttatttt
    10261 tgtttttcca gaacgttttg ctccatgtaa taaagtaata aaccaatcat tcttaatggt
    10321 tttatttaac acgtcgattt gctttgggtt ataaatatca actagtgcca tctaattcac
    10381 cactaatctt ttctaacaac tcatcaagtt tttcttcggt agaacgctct gagtttatct
    10441 gtaattgttc taattttgct tgcattaatt ttatttctgt ttgagctttt tctaattgcg
    10501 tttgtgataa cagcgattta tacttcaaat acaaatccaa ggctttcaat ttgctttcta
    10561 aatctggtgt atattcatat tgcatatgtt taataacctt gtttttctcc aagcggtcta
    10621 tttgcttgct cacgcttatt tgtacttctc cttgccaaat atctatcaat tcatttaaag
    10681 cgtcctcggc acttaatttg cgcttctttt cgataggatt tagccgttca ttaatatact
    10741 gtattatgtt aggtttagtt aggttttcct tacctattac tcttgctgat cgtttgctat
    10801 aacctgcttt aatggcggct tgagtagcat taccaccatt ttttatatat tcatccgcaa
    10861 aagctttttg ttttggtgtc aaatttacca cattcaagcc accacctttc aattttatta
    10921 acaatatctc tcaatatttg tctgtatgtt ctgctcacta aaatatccat ggccgcagta
    10981 acgaagattg tacttatcaa tctcttttgg cgtagcttct ctggtcattt caatgatgga
    11041 gtacttcttt ttaatctgga ctgactggac aactctaatt ggatcatctg cgtttggttg
    11101 cggatattta tttgataatg atacatacca gtagttcctc attacgtatc actctttctg
    11161 ttggttactg gcagaaaagg ttcgcatcat aaattccata gcctgtgcgt cattaaatcc
    11221 ttgcagaata agctgatcgt agaaatactt agcttgtttt gcgattaacg ctaagctttt
    11281 ttgagtttcg tagaatgtcg cctccattgt tttgtttgct tcattcgttt taaacaactc
    11341 tcttaattgg ttttcgttca tttttttact ccccttccaa aataaaaaga ccactcgatg
    11401 agtgatctaa tatgtaaaaa ctacacctca taaacgagat gcagttatag ttctatcaag
    11461 caacctacac cgattccatc gattactatc gacctcgcct tgctcgtgta ctttgagcgc
    11521 ccatttccaa ccctcagttg ccaaagtcac tggcaaggaa tcgaaccttg catggttgcc
    11581 gaagcattga cctaacacac atgcttagcg tctacccttt ccgccacagt gacaaattaa
    11641 tattgtgaaa ataaatacta agtgtataat tttatttatc agcgagtggt ccgctgaaat
    11701 aaattaaaag gtggtgaaaa atttgtactt tgttataaga gaagcaacta atggccaata
    11761 ttacttcgtt attaagtccg ataacaatga agtagtagca actagcgaaa catacttgac
    11821 taaatattcc gctgaacaaa ccattaattc tataaaaaac ggaatcacca aagactctca
    11881 agttatcgat gtgactaaat aagacgacta acttcatttg ctagggagtt catttcagct
    11941 gctttttcta ccaaagcagt tgagatactt tctagctttt ctgatttaac agaattaaat
    12001 actggcgtct tgatactaat cgatacatct tgacgaggat caggactcgt tgctctattt
    12061 ttttgcacca ctgatctgta cttttctact gaatgatact caattacttg ttctaaatgt
    12121 tttttgtact ctttacatac agcttctttt tggatcaatt ctaaacgttg ttgctcaata
    12181 atatcaatca accgttcccg atccatacct tgatacttta tttgctcgac ttccattcgt
    12241 tttccctcca atacataaat taataaacag caacggatga tagataataa gaacaattta
    12301 gaaggagttg gaattcacat ccttattctt aatatttccg ttgctgtcta tcgaagctta
    12361 attgtgaaac aataataaaa cgatgttcct tttattatta ttttgtctca gacctatcac
    12421 taatctttcg acactatcat aataaatcat ttaccaggta agtgattggt ataaaaaagg
    12481 tataaaatgg aaaccaaatg ggtaatcaaa gggtataaaa agtgtaaaaa ctggctactt
    12541 aaaagcaacc agttctaacg atgaagcaaa ttggatgata attctgtttg attctacttt
    12601 aaccgattct tcgctagtat tattcctttg agcagttaca taaatgggca gaccattgat
    12661 ataacgatca tagaatatct tcttgcgcct ttctgttaca tcaggtttat gcggatgctg
    12721 aattgctgaa tagccacgaa caaataattt gtgcaagtac tcaaattctt cttgcgcttc
    12781 ctctctctca atcatcattc gttctgcttc aaatgtatta tttgctgtag aaggtggaac
    12841 caaggagtaa gatgccgtta ctttaggctc ccgaggttga cctatcctac atctagctga
    12901 cagatatgct gaaagaaaaa caccaacatt atgttttgtg cgatccatat ccacatcttt
    12961 tgcctctggt gtctcatact tctttacatc gaaaagtacc atccattgat tcctccattt
    13021 atgatataat aattgtgtca gaattattaa ttaaggtcgg aggaatccgg ctttttttat
    13081 acccatgctt atgctaagct tttcaagtag cgaggttgca ctcgttaccc atatacatgt
    13141 tgagctatct ggcggaaaac agatggctca ctatttcaat attctgctaa ggacagccag
    13201 tggtcggctg tcttttttat attttaatga gaagccttac ttattttttt atctttatta
    13261 cgaaaaatga tattatctat tgataataaa gcgtatagga gatggttgag tgactatgtg
    13321 gagcatgctg ttattttgga ttcccgtttg tattggtatc gtcgcatttt gttactttgt
    13381 caaacactct agaacaaata agctcctcat gttatctttt ttacctatag tattttttat
    13441 tgtacaaatt gttaaatata cctatattga atcgcaagaa atattcattt tttatgtggt
    13501 aggtttattt atctctgtgg tctttttcat aatgatactt tcctattttt ataaaaaata
    13561 aatttttctc ttagaagtac attttttcgc tgtttatata gccttcatat ccaccaatct
    13621 cgctacagct aaattctctt tgcttttcgc taaccactta tcacattcca tcgtgttttc
    13681 aatacgaatg atcgctgagt gattatagac gtgttctaca tatccacgaa atggatagat
    13741 gaacccttct gcttcgcagc gaaccatgtc accgactttg aattttggtt tcttacgttt
    13801 tttaggattc tttgtcggca tatctagcat taaaccgccg ataccatgac tactagcgta
    13861 aaatccgtct tttaatttca tctttctacc tcctcatgat cgacagtgac cagttcatat
    13921 acttgtgtta atccaccaag tcgtcgtgaa acttggttag cttcttcgag gctatcaaac
    13981 catctcgcac tttggagtat gtctacaatg aataatgtgt tagcccctat agagcgttca
    14041 tcacttctat aaaatttatt cccgaacttt actacataaa tcttcattct gcttcctcct
    14101 caatcgtcca agcatttgta aatttctcta agcttattag ttcatcttca tttaaatatc
    14161 cgtaaccata taaggattct aaaaggtctt cttttgaata gcctctaaaa tcaagtctga
    14221 gaccgcttag gatgtataca taggttttat ttccataaaa cttgattgtg tatgctttca
    14281 tcccacttcc tcctcatcga tctctatctc gacatctctg ccttctaaaa tctgtttggt
    14341 atcaatatag gctacgagta tttttcgatt ttggcttaaa gcaatatacg gatcaatcaa
    14401 agcaacttct gcgtgtagac gataataagc aatacaatct atttctaaaa atatcaaacg
    14461 atcaatagca tcattaatca taaaatctgt aacgttttct gctgtattta aaccatctgc
    14521 atatttaggc aggacttcat tattgatgaa ctcagctaca tgttttggac ataaatcagt
    14581 cagttcttgt tgtgtatagt tcatttgctg tcctcctgat taatatattt cctcaaacta
    14641 cgtatccctt taccacgttt ttttgttttg cgctcaatcc acgttcctgt agttccccag
    14701 tagtcaataa ttccattatg ggtattaatt gcaaattgat gattgtttgg ataatgagta
    14761 aattcaaatc ccaaggtttc aatattttta gttgctgaat tgcccatctt tctgacatgt
    14821 tgttttctac gttcttttag atgcggttta atatctttcc agtattcgcc catatcgccc
    14881 atttcgctgt cctcctatct ccttgtccac aataaaaacc acatcaactc ttctgtcgta
    14941 ccgtttaaat aaagatttat ttctgcacca atcgcaacta aaattccgac aacaaacaaa
    15001 ataatcccta ctgctgtata ttttttgcct tttgcactct cgtaaatgag gtatgctcct
    15061 aacatcgcta gtaagccgcc aattacacac caaaatattg ttaggctgat catttgctgt
    15121 cctccaaatc actcgacttc acgaatacac catctaccat ttttcctgtg cgtcctttga
    15181 tttcgttgta tgcttggttc agacactcgt acaaatccat atcattttgc atagctaaaa
    15241 taatcaaggt tactactaca tctccaattc cgtctcttaa accatgttca tcttttctag
    15301 ctagagaagc ggcaacttcc ccgatctctt cgatcgtttt taacatttgc ttgctggaat
    15361 cagcttgatc caatccctta tctttagccc actgctctac ttttgtgatt agttcgtcca
    15421 ttattcattc tcctttatat atttaagttg gataatacaa cttgtaacaa atgaagctaa
    15481 tgttaaaatt gtgccgattg gtgttaaaaa accgcttttt atcgcattaa ttaatatagc
    15541 tacaaatatc gttatataga ataaaaagtg aattgacgct aacgttgaat taaacatcta
    15601 gtcctcctcg aaatactcat tcagtatctc tctatacttt tctacaaatt tgaaacgatc
    15661 ctgatgaagt ttcttgctcc aatttgtttg ccgatccagc tcacgcatct gatcgaaccc
    15721 tttttgaatt tcgttgtaat aaaattcaat gtttgctgct gctttccaat gcctgctact
    15781 tcgcactcct gctcctgttt cagccatttc caacttaact aattccgctc gttcttttgc
    15841 ttttttatct ttctgaatct tcatcatgat tttcttgagg atgatatcac tgtattgtgt
    15901 aatgagatcc attatttctc ctccacatac ctaaactgtc gcccctttga atcaatccat
    15961 aagctcctag ctctatccca gatgatgttt ttgctcagtc cagtaatttc agataactgt
    16021 tcagctgtac ccgttactag aattcgatca ccatgccaga ttgcaatttt tctcggcgtt
    16081 ttccgtttag gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt
    16141 tctttgtctt cttgccaatt ctcagaatgt gtcagttcaa tgattcgttt cattgctgct
    16201 ttcttaccca cgctcattcc tccaatctac gaatttccct tcttaaattc actatgtgca
    16261 aattgattgc ctttctcgcc gtttcattga ccatcactgc ctttatccgt tccagatcgt
    16321 caatctcacg ttgaaggctt cgaatacgca tttgaatcac ttcttctgtt gtcatgatgg
    16381 accacctcgt taaaaacgct cttccttgaa cgtattccga tattttttag ctaatatcaa
    16441 cggcacttga tattgatgac agaacaactt tgccttgatc ttaaagtctt ttgtctgcat
    16501 tcctttgaca tctacgactt tgacaagttt gccgttttta taaaatgtga agtcgggaat
    16561 atactcgatc ttgcgatact tttttccatc tagttcaaat ttcggcatca gctcaaatct
    16621 ttcctgaagt tttactttcc agccgttcgc ttctgcttgc cacaaggcta aatcgtaata
    16681 ctctgcttcc gcgatagaat caaacttgat acctcgatga acagttttct tattacggta
    16741 tttattcatg cgatactacc tttcactggt tttatgcgct tgtctgctgt ttgttggaat
    16801 ttcagcgcat aaccttctga attcttaaat attctagaaa caattctttc gccgtaggct
    16861 tctcttagtt caggaccgga taagtttgtt gtgatgatcg ttgccttgtt ctgtctggct
    16921 tctaagagcg tgtttaacgt gttgtttgta aactgcctac tatttgatac cccgctacct
    16981 aattcagctc caatatcgtc aaaaaccacc aaatcagttg ttttgatatc ggctataagc
    17041 gatccttcaa tttcttttct cagttcagca ttgttataag aaaactttat ttgctctaat
    17101 aactcttgat agcttataaa aagtattttc ttgtcataat ttgagcgctc aagtatttcc
    17161 caagctgtcg ccattgacaa gtggcttttt ccgcttcctg atttccctga tagaatgaaa
    17221 tgtgcaggat ggttcagtaa gacatcattt acaaagcttt tagctctttc taaagcaatt
    17281 ttcgtttctt ggtccactac gtgataattc tccattttgc atttaaacaa agttttatct
    17341 gttaataccg aaccattttg aaaaaaactc aacgctcgtg cttttaagct gtcgttatat
    17401 atccgttcgg tctgtatatc ctctttcaca cgtaacgctt tataaccaca actcatgcat
    17461 gttggtttac aacgttctga accatcctta tttttagctc gccaactata caaaggttcg
    17521 ctacattctg gacatttccc gctttgcact aatactcttc ttattagttt ctccatagca
    17581 tttgctaggc tttccatgtg atgcatctcc tttttaaatt ggcaagtcgt catattcact
    17641 aggattgctg tactgtagtt tttgactttg ctttttatga ttattcttgt ctgctttgat
    17701 ttcgaatttg agcttctcaa atttttctct caatttctta gcacttctaa tatttccaaa
    17761 ccaaaattca tttgtaggta gccaattgat cacatactca atcgcttcta tagacgcttt
    17821 atctctttct tccatcaacc tgattgtgtc tgcccatttt tcgatatcta ctttgttcat
    17881 ttcttttgga aaatcttcag ttaaattact ttgcaatttt ttagcaaggc gtaagtgttc
    17941 gtcagaatac ttacctttct tttcttcttt atctatatct atatctttct ctatctctat
    18001 ctctaactct ggtgtagttt tgtctggaca tttgtccgac acttgtcctc cagttattaa
    18061 attccgtttt gcctcttcta ttttctttct gtattctctt tttctatctg cttcagttga
    18121 ggattttcca atgaaacttt gtatatcaga catataaatt gctccgttat ctaatacgtc
    18181 aataagctgc aaatcacgga aaatttgtac cgctttttct acgactccta cagaatgtct
    18241 tgtaatagtt gcgagcattg tagagttaaa tggaatcctg tcattaaaca tcaacttacc
    18301 ttcgtgtttt agacttctta aataaagttt gagaagaata ttagaataaa tatagccatc
    18361 tggcatactt tctaagagaa ccatctcgtc actatcgaaa aaattctctt ttagttttaa
    18421 atagtagtag cgtttgttgt cagacaatat tttttaccct cctattctaa gtttcttaat
    18481 tgtttcctgg tttaacttga tccctttgat ttgatattta tttttgaaat tgatcacacc
    18541 tatattgtgt ttctccgtgt gatgaatcct gcagagtgct gcaaatgtgt actctgaatg
    18601 atcaacttct ttgcgctttc gtcttcctag cgctttgtca aagtgatcga tgtcagctcc
    18661 tgttttgcca cagatgcagc agactctttt tgtgatgcat ttgtagaagt aatattcttg
    18721 attcgctggt aaaatctcat agccttcttt gaaaggaata tgatgttcaa agatgaaatc
    18781 taagatgata tttgctaaga cattagcatc actcacagtt gtattcgatt cgtctttgag
    18841 gcttattttg cgccctgtga cgccttcaaa acggaagtaa aagaattcct tccagaagtc
    18901 cgttggcatg cctgtatcga tgaaaatatc gcctatcagc gcatagatga agtttcgttg
    18961 ctgcacagta aatcgacgtg gatcaataaa tcgaacttca ataatccgat cgccatcata
    19021 tccgtcatac atcgtcttta gtcgatcaat gttcacttct tcattgatcg ttgcacctat
    19081 gtcttttcct ttgaactttt tcagaaccgc tgaatatgaa tcgatcaatg gtttaaacac
    19141 tcatatcact tctcttttgt ttcttctctg tactgatctt caatccagtt aacgcctcgt
    19201 tttagaatgc ccaagtctct cttggtccat ttactgtcat cagcggttat agaagccgca
    19261 tcagtcaatg caacaattgc ttcatcaatt gatttttcgt acttgttagc aaccagttgt
    19321 aaagcatcca agaatagctt tttgcttctt tgagtagctg gttcaagcat cgagacatct
    19381 tctggcatat cttcgccagc aaatatatat agccctagcc caaacattgc tagatttttt
    19441 acaagacagc gcatgatcgt tttgttgata tcaaacatag ttgctgcttc aactcgcttt
    19501 tcgatttttc caacaatctc ttttttcttc gtttcgttat tccactgata atcattgact
    19561 tcgtaggtat atggctcatc tttcattgcc ttgtttgcac catccatgac tggtaaccac
    19621 atgtcacgct ttactccgtt gactgtgata ctggtaaaaa ccatatagcc tgttttctta
    19681 tcaaagacat acggcagata tgtttctgga tcatgataga tttcataatc tactgcctca
    19741 cacactttgc taacttctgc ccaagcccat gcccacgaaa gataagtgag gttatttcgt
    19801 ttttcagtaa catctttcac atttattttg tataaggtat taaaaagcgt attatcactt
    19861 ctatttttca aaggttgttc cttttcactc atcaaattct gcctccattt cagcaatgta
    19921 tttcttacct gatccgtaat aagagatatc aatcaagtta tctctgtcat actcttctag
    19981 cgcatcaatc aagccatctt cgatgacata gatatattca gtttttttgg acttcctcga
    20041 taaatggata agatagacat gatcccaaat actcacaaaa tttcccaaat cgtcttgatc
    20101 acatgctagt tcttcatccg tcaaaagatt acgtctgatt tttcgattgc ttgtttcctt
    20161 gatattcgat ttgccccaac taggatcagt caaatattga tctagagtgg aaagttcttt
    20221 tttcatgtgg taacatctcc ttagatgtat tttctttgtg actcattgct ttggtcggct
    20281 gagtcacttt tttatttgtt gccatgcttt ttgcttatca atatgttgtt ggcttaggat
    20341 gtttggttta ttgtgtctcc accagcgatt agcaattatt acgcctattt ttagcgcttc
    20401 agctctattc atcctctaac aactccattt gcttaataat tgtcatcgtc gctgtagaag
    20461 gcatccaatt gttaataaaa tcgataacat catcgaactg ttttgccttg atacgatttc
    20521 tagctactgc tccagtgatt cgcttaattc cgccattcaa gtctttaaac aattctgctt
    20581 ttgctttgct atttaaatgc tgctgattac aaataactct aattttgttg cgaaccatat
    20641 tagaaattgt tcggtaatct ggttcagcta gaagttgatt gttcttcaaa tcgtttaaat
    20701 cagactcgat agcgtctact ctttcattcg tttcttcgtt tgcagctaga gcaagtaatg
    20761 ccatttctcg tttagttgct ggaattgatg gcaattggtc ttttaattct ttttccatct
    20821 tgttgaaggc ctcaatatat tgaagtttaa aacttaaagc tttcttacca gtgaagccca
    20881 ttgctaatag agagaagccg tctctattca tataaataat tcggtatgat tgtttatttt
    20941 gaggatgaac ataagtatct tcccaaaata ggtctgccca attttcggca accccctctt
    21001 ttagttcatc aatagccgtc aaaacatgtc tgtgcttttt atcgaagctt tctgcaactt
    21061 gtaagctcgt agtcacagct tctttatttt tcaaaattac taattcttgc attatttctt
    21121 ctctcctttt tgatataatt ctggtggggt gaaaacatga gcagaaaaat tgataatata
    21181 atattgttaa ca
//
LOCUS       DACDOA010000047        20181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDOA010000047 DACDOA010000000
VERSION     DACDOA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.047"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IWI39_001572"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IWI39_001572"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9208682.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IWI39_001573"
     CDS             1011..2258
                     /locus_tag="IWI39_001573"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9208683.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IWI39_001574"
     CDS             2334..2678
                     /locus_tag="IWI39_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208684.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IWI39_001575"
     CDS             complement(2795..3121)
                     /locus_tag="IWI39_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208685.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="IWI39_001576"
     CDS             complement(3494..4696)
                     /locus_tag="IWI39_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208686.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="IWI39_001577"
     CDS             4813..5328
                     /locus_tag="IWI39_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9208687.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="IWI39_001578"
     CDS             complement(5527..5973)
                     /locus_tag="IWI39_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9208688.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IWI39_001579"
     CDS             complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IWI39_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9208689.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="IWI39_001580"
     CDS             complement(7852..8481)
                     /locus_tag="IWI39_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9208690.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="IWI39_001581"
     CDS             complement(8465..8929)
                     /locus_tag="IWI39_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9208691.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="IWI39_001582"
     CDS             complement(9416..10684)
                     /locus_tag="IWI39_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9208692.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="IWI39_001583"
     CDS             complement(10915..11652)
                     /locus_tag="IWI39_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9208693.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="IWI39_001584"
     CDS             complement(11719..12288)
                     /locus_tag="IWI39_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9208694.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="IWI39_001585"
     CDS             12416..13288
                     /locus_tag="IWI39_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9208695.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="IWI39_001586"
     CDS             13630..14502
                     /locus_tag="IWI39_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9208696.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="IWI39_001587"
     CDS             14499..16457
                     /locus_tag="IWI39_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9208697.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="IWI39_001588"
     CDS             complement(16708..17559)
                     /locus_tag="IWI39_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9208698.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="IWI39_001589"
     CDS             17682..18149
                     /locus_tag="IWI39_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9208699.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="IWI39_001590"
     CDS             complement(18412..18894)
                     /locus_tag="IWI39_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9208700.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="IWI39_001591"
     CDS             complement(18994..19857)
                     /locus_tag="IWI39_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9208701.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DACDOA010000048        20020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDOA010000048 DACDOA010000000
VERSION     DACDOA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.048"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..3358
                     /locus_tag="IWI39_001592"
     CDS             5..3358
                     /locus_tag="IWI39_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9208702.1"
                     /translation="MLIILSLSNFVPLSAIADTTDDPTVLETISAEVISDQSGKKALN
                     IKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEISSNTKQTIDL
                     SFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEESSKENENSVS
                     PFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDHQGNKNGTNQW
                     DGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYLNARGNVQKDI
                     TPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGYVGYSSEGYSY
                     SNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGHKKVIVLLTDG
                     VPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNNLSRRIDSTFI
                     ATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDEKGDLYYESAD
                     HATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTSPTTVTPSISI
                     EGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQPNIDTNELAE
                     FGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAWKNGYIRIIKP
                     AKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKELPVPGYDSQQI
                     DAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDHGDGTYSLPEN
                     VKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTSDDTIQLTIEN
                     PFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTNEKGLASFDSL
                     TPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWTLTHQNRLKAF
                     DLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPGTYTLTETFTP
                     EGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQAKVPLPETGGI
                     GRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3361..4782
                     /locus_tag="IWI39_001593"
     CDS             3361..4782
                     /locus_tag="IWI39_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9208703.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4779..6656
                     /locus_tag="IWI39_001594"
     CDS             4779..6656
                     /locus_tag="IWI39_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9208704.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6748..7590
                     /locus_tag="IWI39_001595"
     CDS             6748..7590
                     /locus_tag="IWI39_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9208705.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7742..8617)
                     /locus_tag="IWI39_001596"
     CDS             complement(7742..8617)
                     /locus_tag="IWI39_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9208706.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8824..9855
                     /locus_tag="IWI39_001597"
     CDS             8824..9855
                     /locus_tag="IWI39_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9208707.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9944..10249
                     /locus_tag="IWI39_001598"
     CDS             9944..10249
                     /locus_tag="IWI39_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9208708.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10428..12443
                     /locus_tag="IWI39_001599"
     CDS             10428..12443
                     /locus_tag="IWI39_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9208709.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12445..12729
                     /locus_tag="IWI39_001600"
     CDS             12445..12729
                     /locus_tag="IWI39_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9208710.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12755..14128
                     /locus_tag="IWI39_001601"
     CDS             12755..14128
                     /locus_tag="IWI39_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9208711.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14211..16226)
                     /locus_tag="IWI39_001602"
     CDS             complement(14211..16226)
                     /locus_tag="IWI39_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HAP9208712.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQTSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKNWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16248..17462)
                     /locus_tag="IWI39_001603"
     CDS             complement(16248..17462)
                     /locus_tag="IWI39_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208713.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17683..18627
                     /locus_tag="IWI39_001604"
     CDS             17683..18627
                     /locus_tag="IWI39_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9208714.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18923..19867
                     /locus_tag="IWI39_001605"
     CDS             18923..19867
                     /locus_tag="IWI39_001605"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9208715.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6819 a         3685 c         3824 g         5692 t
ORIGIN      
        1 tttcatgctg ataatattga gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac
       61 tacagatgat ccaacagttt tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg
      121 aaaaaaagca ctgaacatca agctaaatgc gaataacacc agtgctgaaa agatagaaaa
      181 agaaattggt ctagtcgaaa attacttaag tgatgtggaa agaaaagaag gagatggcta
      241 tgcttatcag gtaaatagcg ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac
      301 tatcgatctg agttttccaa tcgatccagc actttaccac agccaggcaa acaagctgat
      361 cgtcgataat aaagaatatg acattattga tgagacagaa aataagaaag atacagatgt
      421 gtcagtacca aagccagacg aaatagaaga agaatcatca aaagaaaacg aaaattctgt
      481 cagcccattt acattgccta cattatcctt gccagctgtg agtgtgccat ctaatcaaac
      541 gattcctaca gaatatacaa cagatgatca gggcacttat cctaaagcca gttggcaacc
      601 tacaggaaat acaaatgttc ttgatcatca aggcaataaa aacggaacaa atcaatggga
      661 tggtataaat tcttggaatg gagatcctaa tgatcggacc cattcgtata tcgaatatgg
      721 aggaaccggt aatcaagcag actatgcgat acgaaagtat gcaaaggaaa caagtacacc
      781 cggattgttt gatgtttatt tgaatgctcg tggaaatgta caaaaagata tcacgcctct
      841 tgatctcgta ttggtcgtag actggtcagg aagtatgaac gacaataatc ggatcggtga
      901 agtaaagatt ggtgtcgatc gttttgtcga tactttagca gatagcggta tcacagacaa
      961 aatcaatatg ggatatgtcg gctactcaag cgaaggatat agctacagta acggtgcagt
     1021 acagatgggt tcatttgatt cagtgaaaaa tcaagtaaaa tccattacac cttcacggac
     1081 aaatggtggt acttttacac aaaaagcact aagagatgca ggaagcatgc tatccgttcc
     1141 aaatggacat aaaaaagtga tcgttttgct gacggatggt gtaccaacat tttcctataa
     1201 agtacagcgg gtacacgcac aatcaagcag caattattac ggaactcagt tttctaatac
     1261 gcaagatcgg ccgggaaata cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa
     1321 caatctatcc agaagaatcg acagtacgtt tatcgcaacc atcggagaag cgatggcact
     1381 caaagaacga ggaatcgaaa tacatggtct tggcatccaa cttcaaagcg atccggcagc
     1441 tggtctctca aaagcagaag tagagtctcg tatgcgacaa atggtttcat cagatgaaaa
     1501 aggcgatctt tactatgaat cagctgatca tgcaacagat atctctgaat acctagccaa
     1561 aaaagctgta cagatctcag caactgtaag caatggacaa ataaatgatc caatcgcaga
     1621 accattcatt tatcagcctg gtacactttc agtcaagagt gtggggacaa gtcctacaac
     1681 ggtcactcca tctatttcca tagaaggaaa taccatcaag agcaatcaga tctatttagg
     1741 aaaagaccaa gaaatccaaa tccattacca agtgagaatc caaacagaaa atgaggactt
     1801 ccatccaaat ttctggtatc aaatgaacgg caggacaact ttccagccaa acattgatac
     1861 caatgaatta gctgaattcg gtataccatc tgctaaagct cccggagtca gtcttcacat
     1921 caaaaagtta tgggaagaat ttgacaacaa tctagctgat cgtccagatc aagttacttt
     1981 tgagattcaa cgggaacata cgacaaatgc tgcagcttgg aaaaacggat atattcgaat
     2041 cattaaacca gctaaagata caacaaatac gtgggaacgt gcagacattg acaaattatc
     2101 tgcaaatagc ggagaaagtt atcaagagat attatcacta cctcaataca ataatcaagg
     2161 tcaagcattc agttaccaaa caatcaaaga attacctgta ccaggatacg attctcaaca
     2221 aatagatgca atgacatgga aaaatactaa acaattcaca ccgttaaact tgaaaataac
     2281 gaaaaattcc tctacaggtg aaaaggatct tattggcgct gttttcaaat taacaggaga
     2341 ttctattgat actttactaa cagatcatgg cgacggaacc tattctcttc cagaaaatgt
     2401 caaattgcaa aaagaaatga cctatacgct gacagaaaca aaagctccag aagggcatgg
     2461 attaagcaaa aagactactt gggaaatcaa gatcgcttct gatggtacgg taaccattga
     2521 tggaaaaaca gtcactactt ccgatgatac gatccagttg actattgaaa atccttttgt
     2581 tgaagttcct gtagcagtac gtaagtatgc gatgcaaggg acggacaaag agataaatct
     2641 taaaggagca gcattttccc tacagaaaaa agaagcaaat ggtacttatc agccaattga
     2701 cagccaaaca acgaatgaaa aaggtcttgc cagttttgat tcactcacac ctggtaaata
     2761 tcgagtcgtt gaaacagctg gtcctgccgg atatgatact tcgccgggaa attatgaatt
     2821 ccaaatcgat aaatatggaa aaatcattta cacgggaaaa aataccgaga tgacaaataa
     2881 tgtatggacg ctcactcatc aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa
     2941 agaagacaac ggacagacat taaaaggagc aaaattcaga ctgcagggac cagaaatgga
     3001 cttagaatcg ccaaaagatg gacaagaaac agataccttt ctattcgaaa atttaaaacc
     3061 tggaacttat acgctgaccg aaacttttac accagaagga taccaaggtc taaaagagcc
     3121 agttactata gttatacacg aagatgggtc aattcaagtg gatggacaag atcatgaatc
     3181 tgttctgtca ccaggagcca aaaacaacca gatttcttta gacatcacga atcaggcaaa
     3241 agtaccatta cctgaaacgg gaggaattgg ccgtttagga atctatctag tagggatgat
     3301 tggttgtgcg ttttctattt ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat
     3361 atgaaaaaac ttggttggct tagtatgtgt ctcttcttgt tactatttaa accagctttt
     3421 actcaggtag caacagaaac agaaacagaa atggttcaga ttactttaca caaattgctt
     3481 ttcccaaacg ggcaactgcc gaaaaatcat ccaaatgacg gacaagaaaa agctttatta
     3541 caaacgtatc gaggattaaa tggtgtcaca ttccaagttt atgatgtcac agattctttt
     3601 taccatctac gggaaaaggg caaaacggta gaagaagcac aagcagagat cgcaaaaaac
     3661 ggtgcgtctt ccggtatgtt taccgcagaa gcaacaacta caactcttaa caacgaagat
     3721 ggtatcgctt ctttttctct ggccgctaaa gatcaagaaa aaagagataa agcgtatctt
     3781 ttcattgaat ccaaagtacc agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt
     3841 cttcctgtac atggacaaaa caatcaaaaa ctttcaacta tccatttgta tcctaaaaat
     3901 gaagaaaacg actaccctga tccacctttt gagaaggtat tagaagagcc tagaaatgat
     3961 tttacgattg gtgaaaaaat cacttattcc ttgcatacga caattcctgt aaatatcctt
     4021 gactatcaaa agttcgaatt gtcagatagt gcggatgaag cattaacgtt tttacctaat
     4081 agtttaacga tttcatcgaa tggagaaaag ctgacagaag gctttgtcat acacaagaaa
     4141 cctcacggat ttgatgtttt attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa
     4201 ctgaccattt cttatcagat gcagctaagc agtacagcac aggcgaacaa ggaaatcaac
     4261 aacaacggaa cactggattt tggttttggt gtcagtacaa agaaagtctc tgtatataca
     4321 gggagtaagc aatttgtcaa aatcgagaca aataaaccag ataaacgatt agctggcgca
     4381 gtattcctta ttaaaaacaa agcaggaaat tacctccagc aaacagccaa cggatacaag
     4441 tggacaaaga acgaatcaga tgcgcttcac ctgatttccg ataaaaatgg cgctttttca
     4501 atttccgggt tgaaaacagg aagttatcga ttaaaagaga tcgaagcacc ttctggttat
     4561 attttaagtg aaacagaaat tccgtttacc atttcaactt ttctttctga ggataaagag
     4621 gcggacagta tattgaaagt agtcaataaa aaagaaaata gccgtccatt tcttccaaaa
     4681 acaaacgaaa cgaaaaatac acttttaggc gttgttggta tggtattcgc aagctttgca
     4741 atctggttgt ttatcaaaaa aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa
     4801 acgttatgtt aggagtcctt ttccttattt taccattact cacaaacagc ttcggcgcaa
     4861 aaaaagtgtt tgcagaggag acagcagctc aagtcatcct tcataaaaag aaaatgactg
     4921 atttacccga tcctttaatc caaaacagcg ggaaagaaat gagcgaattc gatcaatacc
     4981 aaggattagc cgatatttca ttttcagttt ataacgtcac tcaagaattt tatgcgcaac
     5041 gagataaagg agcgtccgtg gatgcagcaa aacaagcagt ccagtctttg actcctggta
     5101 caccagttgc ttcaggaacg acagatgctg atggaaatgt cactttatct ttacctaaaa
     5161 aacaaaatgg gaaagatgca gtctacacga tcaaagaaga accaaaagac ggagtgtcag
     5221 ctgccgcaaa catggtttta gctttccctg tatatgagat gatcaaacaa gcagatggct
     5281 cttataaata cgggacagaa gaactagata ctatccatct ctaccctaaa aatacagtcg
     5341 gtaatgatgg aacgttgaaa gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg
     5401 gagcagaatt tattatttct aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa
     5461 gtgtcacaga tggattgtac acttggacaa ctgatcaaac caaagccaaa catttcatta
     5521 ctggtcattc ttatgacatc ggcaacaatg actttgccga ggcatctatt gaaaaaggcc
     5581 agttgatcgt taatcattta gaagttggaa aatataattt agaagaagta aaagctcctg
     5641 ataatgcgga aatgattgaa aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc
     5701 aaacaccagt agaaaagacc atcaaaaatg atacgtctaa agttgataaa acaacacctc
     5761 aattgaatgg aaaagatgtc gcaatcggtg aaaaaattca atatgagatt tctgtcaata
     5821 tcccattagg tatcgctgat aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta
     5881 tcgatactca tgacgctgct ttaacatttg ataatgattc ttcaggaacg tatgcttatg
     5941 ccttatatga tggaaataaa gaaatcgacc cagtaaatta ttctgtcact gagcaaacag
     6001 acggattcac ggtttcagtt gatccgaatt atattccttc attaactcct ggcggtacat
     6061 tgaaattcgt ttactatatg catttgaacg aaaaagcaga tccaaccaaa ggattttcta
     6121 accaagcaaa tgtcgataac gggcatacaa atgatcaaac accaccgtca gtcgatgtcg
     6181 ttactggggg caaacgattt gttaaagtag atggtgacgt tacatcagac caaacacttg
     6241 ctggagcaga attcgtcgtt cgtgatcaag atagtgacac agcgaaatat ttatcgatcg
     6301 acccatccac aaaagccgtc agctgggtat cggcgaaaga atcagcaacg gtttttacaa
     6361 ccacaagtaa cggtttaatc gatgtgacag gtctaaaata tggcacgtac tatctggaag
     6421 aaacgaaagc gccagaaaaa tatgttccat taacaaaccg tgtagcattt actatcgatg
     6481 aacaatctta tgtaacagca ggacagttga tttctcctga aaaaatacca aataaacaca
     6541 aaggtacact tccttcaaca ggcggtaagg gaatctatgt gtatatcggt gcaggagtag
     6601 tccttctact gattgctgga ctgtactttg ctagacgcaa gcacagtcag atttagtctt
     6661 tttgcaagaa ttggaaaaag gagcagcgga ttacgcaaat gctccttttt cttttattat
     6721 caaacagtca tcacaaggag aacacaaatg aaaccaaaga aaaggaaact tctagatctg
     6781 ctgatgcttt tcttgcttct ctcaggaata gggatactag cttatccatt tgttagcgac
     6841 gcgctgaata attatctgga tcagcagatc atctctcatt atcagcaaca agcggtcaaa
     6901 gaaaatgaag aagtcatggc taaaatccaa gaaaacatga caaagaaaaa ccagcaatta
     6961 gcaaaagaag gtggaaatcc aggtgcagat ccctttacta agaaaaaaga acctgcgaaa
     7021 aaggatagaa cctattttga gaaacatacg atcggtattt tgacgatccc tgagatcaat
     7081 gtccatttgc caatttttga tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta
     7141 ttggaaggaa cgtcttatcc tacgggggga gaaaatacac atgccgttat ttccagccat
     7201 cgaggactgc ctcaagcaaa gttgttcaca gacttaccga aactgacttt aggtgattct
     7261 ttctatatcg aaatcaatgg acggacactc gcttatcaag tcgatcagat caagacggta
     7321 gaaccgactg atacaaaaga tctgagaatc gaaaaagggc aggatttcgt gacactccta
     7381 acttgtacac cttatatggt caacagtcat cgcttacttg tcagagggca ccgggttcct
     7441 tatcatgcaa aagaagttca aaaagaagta cagaaagtat ccgagaacaa acgatttttc
     7501 ctgtatggat tgttagctgc atgtatactg attttcctcc ttcttatata tctttttcgt
     7561 caatcgttca aaataagaaa cagaagttag ctattcctca tttctataat atatctattc
     7621 gaatcgttcc gtaaacaaac aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat
     7681 gatgatatca aagagagcct atcaaacact atctttctta ttactaaatg aggatctttt
     7741 cttaagtttt ccgatattct ttaagaaggc ctttttgcat caattcttgt ttgtttttca
     7801 agttgcggat attctcttca aaattttgag aatatatcac tgagaataac gtatttaata
     7861 cgtaatcgaa ggcaatttga gaggagaaag tcgctacttt agcaaaatca tattcctcct
     7921 gcgatataac aagacacata tcagcctgtt tggccatttc tgaatgctga tttccggtaa
     7981 tcagcaaggt aggggcctct acatgcttca aataggtcat tattcgttca taatgatgga
     8041 tgcgaccgct gtagctaata aaaatggcgc aatcctcgct agtcaaattg gctgcattcc
     8101 aagaagaatc actgtattct tcagccatga tcaaaaactt attcaacttg accattttat
     8161 tctggaattt ccttgccgtg atttgagaat caccttttgc aaaaagaaag attcgctgtg
     8221 ccttgttcaa tactttaccg atttgatcta aatcttcatg ttcaatctgc aattgtgctt
     8281 tttttattga ctggatcgat aaatccgcca ttttctttgc aatttgctga gtgctatctt
     8341 cacttgaaaa aggaaaatta ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa
     8401 cttcccgccc cagctcgaaa cggaattctt taaatccgct gaatcccatt tttttgcata
     8461 aacgaatgat agctgaatgg gaagtatacg tagcttttgc taattcttca atcgtcatgt
     8521 agactgcagc ttcgatattc ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa
     8581 acaactcttg ttttgttagt ttttcttcaa taagcatgat ctgacctcca aattctatct
     8641 tactgataaa cacttggttc cgctactatc tttttgattt ttatccttac tttgaacatt
     8701 aatttcacca aaccgaatga taacaagtgt ttttctgaac ttttttacca gaatagctat
     8761 tataaatact ttctgcgctt ttctaggata tactgaaaac atcaataaaa ggaggcacca
     8821 ctcatgctta caatcgctta tcttggcaat ggaaagagta caaatcgcta tcatttgcct
     8881 ttttcaagaa aacttacgaa caaaatcaat atcaaaaaaa ttttttcccc atctgaacca
     8941 gtgtggaaaa cattcgaaga catcgaatat actgatcagc tgtctgatct ttgggatgat
     9001 cctgagatcc aattagttgt catcactaca cctagtcaat ttcattatga atatggaaaa
     9061 atggcactcg aacatggtaa gaatgtacta gtagagaaac cttttgcaga aacgtcaaag
     9121 gaagcagaag agttatttgc attagcaaaa gaaaaaggtc tatttatcca atgttaccaa
     9181 aacagacggt tcgattctga cttcttgaca gtccaaaaag taatcgaaag cggaaaatta
     9241 ggggatattt tagaagtaga aatgcattat gattatttca gaccggaagt accagaaaat
     9301 acgcatgaat tttctactgc gaacagctat ttatatggac atgcttgtca tacagtagat
     9361 caagttcttt cttatttcgg cgaaccagat gatatcagct atgatgtacg tcaactttta
     9421 gggaaaggac gaatgaatga ttacttcgat ctggattttt tctatggaaa tatgaaaatt
     9481 tcgataaaat ccagttattt ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa
     9541 aaagggatgt tcgtgaaaca aacaaaagat cgacaagaag aacatctgaa aatgttttac
     9601 atgcctgatc atgaagactt tggtatcgat ttgcccgaac attatggtgt attgacctat
     9661 atagatgaac aagggagtta tcatgaagaa aaagtaattt ccgaggttgg tgattacagc
     9721 cgtgtctacg aagggattta cgagacatta gttaatggcg cagaaaaagt catcaaagat
     9781 gaagaaacaa tcttacaaat gaaaattttg gaagaaggaa tcaaaagcat tcaagaatca
     9841 gaaggaaaaa tatgacatat ttgtaagaat cgggtttgtt ttttgacgca taaactgtct
     9901 tattgtacga tgaaaaagta tagaactact aggaggaacc aacatgacaa attatgacgg
     9961 tgaaaaacac aaacgattaa gaggttcaat ggcccctaaa acagaagaag aagtggcttt
    10021 agcaggaact aattctggtg aaacagaaga caaagaagaa tcattagctg gcacaaactc
    10081 tggtgaaaca gaaaataaag aaacagcttt atctggttca aattctgtag aagaggaaac
    10141 aaagcctaat gagcccaatc actttgaaaa agatatcgaa aaagcggttg aagatgtcaa
    10201 agaaaaattt gaagaactga cctcaaaaaa taaacatgat catcattgat cggacgaaat
    10261 caattcataa aagcaggaga catttggtca cctgcttttt ttctgtatcc attcgataga
    10321 aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt tgtaccaaac acccctcgat
    10381 ttaaaagcgc taccatacta gaatataagt aggaggtgaa cagcgctgtg aactacaaaa
    10441 ttagttatct cctgaaaaca ccaagagaaa tcagtctaga aaaagcagca gagatcatcg
    10501 aagtaaatac aagcgaggcg atcaaacaat taactccttt tgccgaatat gtggatgata
    10561 cccgtatccg tataaagaaa ttccgagcaa tcgactgggc agaaaaattt ttggatatta
    10621 ctcgatttga agctgtgtat accgaacaag agcgaagaga tcttctttat ttgatggttt
    10681 attcgactgc aacgtctgtt tctgtttttc attttcaaga ttttcttcag gtttctaaag
    10741 ggacagtcct gtcagatatc aaaaagataa gaaatgaatt agaagtggaa gattgtcagc
    10801 tgtcgtatac taggaaaact ggttttaggc tgctagggtc agaagcaata ttaagaagag
    10861 aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc tggaagattt gggctcacgt
    10921 atttaaccgc aaccctttcc attgattatt acgccaaagt tagagatttg ctagataaaa
    10981 cgatttcact atcagatctt cgattcgcac caaatcgaat ggatgaatta gcttattatt
    11041 tgagtttgac aaccattcga attggaaaaa catccatgga actggtaaaa gatgctgaac
    11101 tgttggcttc tcttgcagtc actgacatta gtcgacaagt attgactggc cttggctatt
    11161 ccgaacatca aaattcggag attatatttt tctctctttg cttagcatcg atcgttgaag
    11221 gagatgtcca ggagcctgca cttgattttt tgctggaatg ttcttcagaa gtcatccatc
    11281 gcatggaatt cctgatggcc gtagaatttg cttcgtttcg tgaattattg atgaatgtct
    11341 tttatcactt ggtccctgcc tattttcgta tttcctattc tttttattta cctaatgtga
    11401 tgatcgatca aattaagcat caatatgctt ccatctacga aatgacaaga aaagcattac
    11461 ttccgttaga aagacgaata gggaagagca tccctgaaga agagattggt tttttcacta
    11521 tactatttgg cggagagatc agaaaagcgg acgcagaaga aagaaataga aaaataagag
    11581 ccgtgatcgt ctgtccaagt ggcattagct cttcattgat cttaaaaagt gaacttcagc
    11641 aattattccc gatgatcttt tttacagaga ctaattcagc tgaccgctta cacgaggttg
    11701 acgaaacttc ttacgatatt attttttcta ctgtacctct cacacttact gataagaaaa
    11761 agtctctcta tgttctgcat ccaatcatga ctgacttgga aaaaaatcag ctgatcaatc
    11821 aagtacaaag agattggctg ataccaggat tttcaatgcc ttctgcccaa gaattgttgt
    11881 cggcgctact gccttatatc gaattaaaag aaggcgtaga cgaagaaaag ctctatcaag
    11941 tattaaatcg taaaatgaac aaacttttag aaaagaagga ggatcaacgt cctatgctat
    12001 ctgaattatt aacagaagaa atgatccaat tatcggatca accaaaaaac tggcaagaag
    12061 caatcacatt tgccgcacag ccgctattat cagaaaataa aatagaagaa agttatatca
    12121 aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat ccatattggc gatcacatcg
    12181 cattacctca tgctcgtcca gaagatggcg taaacgaagt agggatgtct ttgctgaaac
    12241 ttcaacaacc tgtggatctg cttgataatc cagcccatcc cattagtttg ttcatctgtt
    12301 tagctgctat cgataatgag gcacatcttc gggctttggc aaatttaaca aagctacttt
    12361 ctaataaaca aaatcttcaa gatttattac acgcatcaac aaaagaagaa atattagcga
    12421 tcataagaaa aggagacgaa tgatatgaaa atttttgcag tatgtcaaag tggattagga
    12481 acaagtttta tggtagagat gaacatcaaa caagcacttg cagatcttgg agcagatgtg
    12541 gacgagtttg aagtaagtca tacggatgtt ggaagtgtat ctagcgatat ggctgattat
    12601 ttcttcattg agcattcttt gaaagatacg ctaacttcta tacctgatga aaagctcgta
    12661 cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa aagtaacgaa gatcttgtca
    12721 aaagaataaa aatgattcaa gggaggaact atcaatggat aaattgttga atttgtttgt
    12781 gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt gcactgcttg gtctcgttct
    12841 tcagaagaag aaattttcag aagtgatcca aggaactatc aaaacatttg tcggattcct
    12901 agtactgatc ggcggtgctg gtttaatcag taattcacta accccttttg cttcgatgtt
    12961 ccaaacagct ctgcatactc aaggagtagt tcctagcaat gaagcagtag tggcgattgc
    13021 tttgcaacaa tacggtacac ctactgcttt gatcatgcta gtagggatga tcgtgaatat
    13081 tttacttgct agattcacaa gatataaatt catttttctt acaggtcaag caatgcttta
    13141 tgtttcttgt ctaacagcag tcattttagt aagcgcgggg tttactgcaa atttgtggat
    13201 gatcttatta ggcggggtct tcgaaggaac actattaact attacgcctg ctctttgtca
    13261 accgtttatg aagaacatca ctggaaacaa tcaagttgcg ttagggcata ccggaaatat
    13321 cggttatgct tctagtggat ggatcggaaa gttttttgga aacaaagacc gttctacaga
    13381 ggacataaag attcctaaaa gtttcagctt tttgcgagat tctactgtat cgatcatgct
    13441 cttgatgtcg atcgtatatg tcgcacttgc tttgttagct ggtcctggat ttgttgaaag
    13501 agaactaagc aatggagaca acgctgtaat ctttgcactg atccaagctg gaacattcac
    13561 agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg ttggcagaaa tcgttccagc
    13621 attccaaggg attgctaaaa aactcgtacc cgattctaaa ccagcattag atgttcctat
    13681 cgtttttcca tatgcaccta atgctgtatt aattggcttt tttgttagct tctttgtagg
    13741 aacactatcc atgtttgcta tgataggctt gcaaactaca gttatcattc ctggggtagt
    13801 aggacatttc ttctgcggag ctgcttctgg tgtcttcggc aactcgactg gcggtagaag
    13861 aggggcggta ttaggttctg ctttcaatag cttattgatt agttggctgc cattatttat
    13921 ccttccagta ttaggagatc ttaaaatcgc ttcttctaca tttgccgata ctgattatct
    13981 agtaccagga cttgtacttg ggaaactagg agaatacggt caaatagcct tgacagttgg
    14041 aatcatcgct ttctttttac ttgtcgtatt atctagcttt ctttttgccg gaaaaagaac
    14101 aaacattgtt tctaaagaac agaattaagc aaacttcaaa ataaaaattg cttggacaat
    14161 atcagaagaa gatttaaagt cttctgatat gtcgcaagca gttttttgtt ttatgcattt
    14221 atttttgaat agcttgttaa gacaagtggc tgttttgcta gaagaggata acttttacca
    14281 ttcattttca ctgttccgcc tgttctatca gaagtaatca cgatcttatc ttttgttttt
    14341 tcaagcgaaa tctttccatc tttgataggt agcgtacaat tcagtgtttc aaatgaatct
    14401 agattaggtt gtatcataaa ttcttcataa cctggctttg ttggatacaa accgataaaa
    14461 tagcgtccta aaagatagat tgggcttgct ccccatgcat ggcataagct ttttccataa
    14521 ggatctccat acatactgta cttttcattt cctgtttgag tagggtcgta ttcttcccag
    14581 aaagttaccg catcatgaga taacatccct ccccaataat caagaatcgt ttggtagacg
    14641 atattcgtct gtccgagttt gcataaagca tcttgttcat agaatttgaa atatggagta
    14701 gtaatctgtg tgatagtgtc attcaataag acattcttta agatcgattg ctgttggtct
    14761 ttgttagcaa aatcgaataa aacagcgaaa atattcgcat gacgcgtcac atttcttctc
    14821 ccagattcat agcaatcgat aaaagcgcca agttcttcat cccaaaagta gtcaaatagg
    14881 ttttgtttca atagttgata tttttcttga tagagatgag tatgataatt ttcgttctct
    14941 ccatatgttt cagctaagat atctccgcaa gtgatcatgg aatgataggc tttcagtaat
    15001 aagacttgtt ctgcagcaag cgttccttct ttatccattt cagaccaatc aataaatatc
    15061 caattttttg ctctcccata aataaaccct aattcattcg tttgctccat caagtatctc
    15121 attaatgatt gcatcttagg gtagacgatg cgtaaaaatt catagtcttt cgtcatcata
    15181 tagtggtttt cgataccaat caaccacaag atcgaatagt caacgatagt attcatatgc
    15241 tgcttgattt cattttgtcc tcttaatgct aatatcgttc gtttattgat ttcctcgtca
    15301 aaaaaaagat attgattgat catgaatgat tgatacgcat ctccagacca aatccagcga
    15361 tctcgtttga taccgtcgat aaaaaacaaa tcactacata acttgaaggc ttcttctgat
    15421 actgaccaga tctgattcag taaagaagaa tcgcttgaaa aagcagcttg tttcgaaaga
    15481 gataaaaatt catgaaaaac ttgaagttga accattccag tagaaacctc tggtataaac
    15541 aaatagcgaa aagcgcgttt acgagctttc atttttcttg tcattcctat ttgcttatag
    15601 tagcaataat cgatatccat agcttctaca tcggactctc cataacatag agtgatagat
    15661 tcgatatggt catccagaga tattagttct acacagccat tcactgctcg cccaaaatcg
    15721 aataagattc ctccgccaat ctccgtttca gtgactggat ggacgcattc tttttgataa
    15781 tagatttgat ttggatcttt tgcgatatcg acataaagcg gactccagcc agctggatat
    15841 tcttcaataa aattacttgc tgtccagcca atatctgatt ttacttcgtc tccctcgata
    15901 aacatcgctg gtagccctga tgtatgtcct acaaagatac gaatttttgc tttaccagct
    15961 ggacaagtca gccattttcc acatggatgt ttttgtccat taatttctac atacccaact
    16021 ccttggatcg tcactttaaa catggaagtt tgatcaagaa agtaatatct cttaaacttg
    16081 acgttccgat ggcaatcatc catgtaccag tacgctggcc agtcaaaacc tcgttcttcc
    16141 cgttggaaat tttgtagcag tccatggcgg atctcaaagt ctccaggata ccaaagccat
    16201 ctatctttat gtttttcatt tgtcatttta cacgacctcc atcaaattta ttttgttgtc
    16261 aattgttctt ttatttcttc atctactctt atcactgtct ttctattcgt cctgtgtact
    16321 actagaaggc taataagcgc gctaatcaac agcaagatac tgccattgac gaaaacagca
    16381 agtgttttac tcccaagaga agcaaaagag agaaagagca aaccatatgg tgcaatgaaa
    16441 gtacctaaat tagcgccaac caataagatt gaagtattga aactctccgc gtacttgggc
    16501 gcttggatac ttacttggtt gaataagtaa ggaatgaata acgaaaaaga aattcctgtt
    16561 aggatcgcac caaacccaat cacatacagg tttatcacag tggcaatcaa tacaaagccg
    16621 attcccatca gtagtaaaga aagtgtaagc atatagcgat gtgcttttgt ctgaagccag
    16681 ccaaaacaaa ctccggcagc catacttgcc aattgcatga ttgttaagat attcgctgca
    16741 ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg ctaatttaat cgatacagca
    16801 ttatatacca cgatgaccag caagagaaaa ataccataac taatcatttt aggattcgtt
    16861 ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa gttcagttgt ttcaggtgta
    16921 ggaacaaaca aaacaaacaa gacaagaata ggtaagctga tggcgtagac taaaaaggta
    16981 tattgccaac cgaactgtac tagctgtcct gcaacaaatg tcattaaagc tgcaccaaat
    17041 ccttgaaatg cactttgaaa accaatcata gaggctcgtt catctccgtg ataaaaggca
    17101 gcaatcatgc tgactgccaa agaattgatc aatccaaagc caatacctag tccagctctt
    17161 gaagctaaga caatactgta actttgtgag aagaggggga ggataccaca aatcgatgca
    17221 atcactaatc cgatgatcac agtttgtttt tgtccgattt tcctagcaat aaaactacta
    17281 atcaaaacaa aaatagtgac cataaaagag ggagcagtcg ttaataattc aacagcagat
    17341 aaagaacgcg tggcataagt agctgacata ctgggaattg cagacgtgac tgcaccagca
    17401 gaagtcaaaa taagagagac tgacagcaga gaaaatttca tcatcttttt attcataagc
    17461 atgttccttt catttttgta agcgcttatc aaaaatatag tatgaagtgg ttattctctc
    17521 aatagcggaa tcacaggaag ttcatgtccc aaccatagaa agtctttatg aaaccaaaaa
    17581 tagctttgca tataatagca gtacagcgtg aactaataag cgaaagaaac cgcttacaaa
    17641 aaggagggag tcaattggaa ctagctgaat tagacatttt ttttgcgtaa cgaagaatca
    17701 atcgaaactc tgcagcgtga acaaggaatg aatatcaatg atttaaactt agcttaccat
    17761 cttcagacac cgccaaaaat ccaccgaggg gatctatttc aacagggaaa tattcatatc
    17821 agtaaacacc gtcgatatgc ggaagttccc gagcatacac atgattttgt tgaaatcaat
    17881 tatatgtata gcggtacatg tttacaaatt attaatggag aaaggataga gatggaagaa
    17941 agtgacgtgg tgatcattga taaagaagct actcaaagaa tcgaatacac agggcaagag
    18001 gatattttaa tcaacatctt attaaaagaa gaaaccttat ctacagagat attgaatcat
    18061 ttggcaagca caaataatct actttcttct tttttgataa atgcatcaaa agaaaaaggg
    18121 gaacatacac aattcattca ttttcgcggc ggaaaaacaa gtcgtttgac gcatttgatt
    18181 gaaaatatgc tgttgattta ttttgataca tacagtcata agatccgttc gttaaatctc
    18241 tatacatcac tattactgat cgaattgact catttattag aacaacaaag cttttcatca
    18301 gcagatggat gcgcagaaaa tgagatgctt agagtattag attatatcga taagcatttt
    18361 acttcttgta cgttaaaaga aacagcagct cattttggct acaatccggt ctatcttagt
    18421 aataaattga aaatgtattt tgatgcttcc tttcaagaac tggttctaaa gaaaaaattc
    18481 gactttgcgt tggaactgat tcaagaaacg agtttttcgt tggaagaaat cgctaatcgt
    18541 atcggatata aacaaacgac ttctttattc aaattattca agaagtattc aacatgtact
    18601 cccaatgaat ggagaaataa acattaagaa gaaaatcttt cactaaagca tatgaaggaa
    18661 aattgtttta ggtgcgattg ctgtcttatt atatcgttgc agcgaatttt tttggattta
    18721 ttttcagttc taaacttttg gttgtattat caggggatat aggtgtaaac atttgtcatt
    18781 tatctgttat gcgtattcag gaattcgttt ccatcaaaaa ttataataac aactattata
    18841 attaacatgt tgataaaatg aataaacaag actatacttt tcatgaaagt aacaaatagt
    18901 tatgaaaagg agctgtttat tcatgaaaaa aacaagtcgt aaagtagtaa tcgtcggtac
    18961 aggttttgtt ggaacaagta tcgcttatgc aatgatcaac caaggaatct ctaatgaatt
    19021 agtattgatc gatgtgaatc aagaaaaagc agaaggggaa gcattggacc ttttagatgg
    19081 tatggcttgg ggcgatgaaa atgtggctgt atggtcaggt ggctatgaag aatgtaaaga
    19141 tgctgatatc gttgttatca cagctggtat caaccaaaaa cctggtcaat ctcgtttaga
    19201 tttagttaaa acaaatgcgt caatcatgcg ccaaatcgta aaagaaatca tgggatcagg
    19261 attcgacggt attatcgttg ttgcttcaaa cccagtagat attttgacat atatcgcttg
    19321 gaacgaatct ggtctgccaa catcacgtgt tatcggtact ggaacaacat tggatacaac
    19381 tcgtttccgt aaagaaatcg cattgaaatt aaaagttgac ccacgcagcg tccacggcta
    19441 tatcttaggt gaacatggtg attcagaagt tgctgcatgg tcacatacaa ctgtcggtgg
    19501 taaaccagtg tttgaaattg tagaaaaaga ccatcgtatt gcccaagatg aattagatgt
    19561 gattgctgat aaagtccgta atgctgctta tgagatcatt gatcgtaaaa aagcaactta
    19621 ctatggtatt ggtatgagta ctgcacgtat cgtaaaagct atcttgaata acgaacaagc
    19681 tgtattgcct gtatctgctt atttaactgg tgaatacgac gaaaaagata tctttacagg
    19741 tgtgccatca atcgttgacg aaaatggtgt tcgtgaagtt gttgaattat caatcaacga
    19801 agaagaaaaa gcaatgttca gcaaatcaac aagtgcttta agagaagtat tgaacactgt
    19861 attataagaa acaatgaata tgaactaaaa atcctaaaac tgcttaacag ctgaaatagc
    19921 tgttaagcag ttttttattg atatctaata ggtaagcaaa taaatagaat ggttaaaatt
    19981 aatcggtccg aaaatgtatc cgtacatatg aacatatgaa
//
LOCUS       DACDOA010000049        19971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDOA010000049 DACDOA010000000
VERSION     DACDOA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.049"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..924)
                     /locus_tag="IWI39_001606"
     CDS             complement(61..924)
                     /locus_tag="IWI39_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9208716.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1120..3219)
                     /locus_tag="IWI39_001607"
     CDS             complement(1120..3219)
                     /locus_tag="IWI39_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9208717.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3439..4548)
                     /gene="rpoD"
                     /locus_tag="IWI39_001608"
     CDS             complement(3439..4548)
                     /gene="rpoD"
                     /locus_tag="IWI39_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9208718.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4572..6413)
                     /locus_tag="IWI39_001609"
     CDS             complement(4572..6413)
                     /locus_tag="IWI39_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9208719.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6595..8988)
                     /locus_tag="IWI39_001610"
     CDS             complement(6595..8988)
                     /locus_tag="IWI39_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9208720.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9095..9955)
                     /locus_tag="IWI39_001611"
     CDS             complement(9095..9955)
                     /locus_tag="IWI39_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9208721.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10014..10535)
                     /locus_tag="IWI39_001612"
     CDS             complement(10014..10535)
                     /locus_tag="IWI39_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9208722.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            10855..11574
                     /locus_tag="IWI39_001613"
     CDS             10855..11574
                     /locus_tag="IWI39_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9208723.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            11600..14191
                     /gene="mprF"
                     /locus_tag="IWI39_001614"
     CDS             11600..14191
                     /gene="mprF"
                     /locus_tag="IWI39_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9208724.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14379..15566)
                     /gene="tuf"
                     /locus_tag="IWI39_001615"
     CDS             complement(14379..15566)
                     /gene="tuf"
                     /locus_tag="IWI39_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9208725.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15934..16290
                     /locus_tag="IWI39_001616"
     CDS             15934..16290
                     /locus_tag="IWI39_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9208726.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16531..17535)
                     /locus_tag="IWI39_001617"
     CDS             complement(16531..17535)
                     /locus_tag="IWI39_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9208727.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            17701..18552
                     /locus_tag="IWI39_001618"
     CDS             17701..18552
                     /locus_tag="IWI39_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9208728.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            18569..18814
                     /locus_tag="IWI39_001619"
     CDS             18569..18814
                     /locus_tag="IWI39_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208729.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19001..19705
                     /locus_tag="IWI39_001620"
     CDS             19001..19705
                     /locus_tag="IWI39_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208730.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5650 a         4085 c         3508 g         6728 t
ORIGIN      
        1 tataacacta tgttaattaa aattattata agctagttaa gaaaaacatg caagggattc
       61 tcaattagat tttttgacta gttttgtcac accgttttct tgtgtaatca atccttgttt
      121 caaaagatta cctaatgcgc gcttaaatgc tcctttgctg ataccaaaag ctttcatgat
      181 ttcttctgga tccgatttat ctgtaaagtt gatttgctga tctgcagaac gttctaaaat
      241 ggttaaaatc atttgtgcat catctgatat agcttcatat gctcttggct tcaatgaaag
      301 attcagtaca ccgtctggtc ggacaccaat cactcgacct tttatctgct cgcctagacg
      361 aggttcatta tatcgttcag aaggatggat aaacccaaga tagaaatctt ccgttaaaac
      421 gtacgtacct gttaatttca agcggtatgc tgttcctatc acatctttgt ttttcatttc
      481 ttctgttcca tatttgctta aagatttgaa tattttttca tctgctaaac tgccccaaat
      541 acggtctttt gcatcaactc taagggaaat catcaagcga tccccttttt taggccatag
      601 ctctgtcatt gtcggcaatt catcaagaga tacgacaaag tctttatctg gaagaccgat
      661 atcgacaaac gcacctaaat cttttcgtgt agcagtaacg attccaaaag cgtaatgacc
      721 gatacggctt ttaggaattt gtgtagtgat ccgattttca tgtttttgat tttggtaacc
      781 gaatccttca actgcttcac cgattttgtg ttcaccttct gatttgctta agccaaaggt
      841 ttgtccgttt ttttggacaa agtagttttt ttcattttca tcaataataa tacctgtaaa
      901 tatagttgct aataaatcgt tcattttttt ctccctctat agtgaaaggt tttcttaata
      961 catttctttg ctatcataac ataatgttat ctacttgaat agctgagcct tggtttggaa
     1021 aggtgaaaaa aggagtatga caacagttgt gtcatactcc cctttttcga ataaacggtg
     1081 ttaaacggca ggctcacagc ctctttcatt tgcctaaaat taattgctcc catttcctcc
     1141 gaaccatcca gttttacagt tgatggttac gatgtaaaat ggcaaagcac tttctccaat
     1201 gcctttcatc aatgcagatt ttgttgaagt ggcatataga ttataatagc cttccccatt
     1261 ttcgattctg gctttttcta atctgtaacc tccttcaacg atatatccac gttggataca
     1321 tgtagcaatc actttgcttt ggacacctgg tgcccacgtc catgctggat tagatgcatc
     1381 ggcaatatca cttgctctgg cagatttgat tggctgattc gctgagtttg aagaggaagg
     1441 ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg
     1501 agctgcagct gctgcttgag cggcttgttt tgcttgttct tcagctgctt ttttcgcttc
     1561 ttctgctttt ttcttttcct cttcttcttt tttctcttta tcagtgagca tcttatctac
     1621 ttgtttcaat tgttcattca actgagcaga tagatctgta tttttcactt tttctactgc
     1681 ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa
     1741 gactatagct gttttttctt tagctgtttg caattcagca tattgatttt tagcttctgt
     1801 ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc gtttcgattg cttggatctc
     1861 tttatctgct ttcaacttga tgtcatctgc tagatgatca tcgacgatgg caggtgctat
     1921 gaaccactca ttcacttttt ggatatctcg gatcttagat tggatatctt ggtaagttgt
     1981 ttctaactct gaatatcctt tttcattctt aagactggat aaatcatttt ttagttttga
     2041 taaatcttga tgaatcatat ctgcacgaat aaattgatgg tgatcatctg tataaaaggc
     2101 agcaagtttc tcatgaagtt cttccatttg ttgctgtctt tttgcttctg ctgctacttc
     2161 ggcttctact ttttgatgat ggtcataata agcccagctt cctccaccga gtagaagtac
     2221 tgctgctaaa acgagataag gtactttttt tgattttttc ttttgcgctt tatctggatc
     2281 tgttttttct aagtttgatt cttgatgtgt tttttcagta tctggcacga ttgttattga
     2341 tttcgcttct gcttcaaaca gcttaggctg gttctcttct ttttctattt ttgcttcaag
     2401 atcagtaccc tctttgctta cagattgatt ttcagcatcg gattcactat ttatttcatc
     2461 gggctctgtt gactttttgt ctgtgttttc gagtatttct tttttctctg cttccggcag
     2521 tttattcgtt ttttcctttt ctaattgttt ttcagtaaat acttcagttt tttctgtgat
     2581 atccgttttt acgtctttta tttttactgt ttctttacct gtggcgtttt ctctatcaga
     2641 gtctttttca gtttcttgtt ccttttctgg ctctactttg ttttcctgtg caattttttc
     2701 actgatcatt ttgagcagct ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt
     2761 gatataagcg gatagtatcg aattttcttc ctcattatcg acgatttctt tcagttcagc
     2821 tactcttctt ttttgttttg attcaagcgt ttcttcttga ttctttcctg attcttccga
     2881 agtctcatga acagttgctt tcttctgttc atttttctct atattagtta cttgatcgtg
     2941 agcaggtggt tcttcgtctt ctggttctgc tttttcgaat aattccatta cagattctag
     3001 aggtaaatct ttcaattccg accattcgat tggatcgttt aattctgtat caggaaagac
     3061 tgcatcttca gttgatttat cctgcatagc tgcatctgta tttgtttctt caattgattc
     3121 ttttatttgc tgatcttctg cagttatttg atgaccgcag tctggacaga tagtattctg
     3181 ctccattttt ttcgatccgc aatttggaca ttttttcatc acttagcgat cggctccttt
     3241 actaggttta cgctaccatt taatctatca tattctatac gtcacgccaa tagcttattt
     3301 ttaggattta cttaaaaata atgaatgaat ttccttccta tagaaaaaaa agcttgagac
     3361 tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa cagttttact gatttctctt
     3421 ctggtcaata gtccacgctt attctaagaa atcttttaat tgttttgaac gagaaggatg
     3481 gcgcaatttt cttaatgctt tagcctcgat ctgacgaata cgttcccgag taacaccaaa
     3541 tactttccct acttcttcca atgttctcgt ccggccatca tctaaaccaa aacgtaagcg
     3601 aaggacattc tcttcccgat cagtcaatgt atctagtaca tcttccaatt gttcttttag
     3661 taactcataa gctgcgtgtt ctgctggact tgttgcttcc tggtcttcta tgaaatcacc
     3721 taagtgagag tcatcttctt caccgattgg cgtttcaagg gaaacaggtt cttgcgcgat
     3781 tttcaagatt tctcggactt tttctgttgg aagatccatt tccgcaccga tttcttctgg
     3841 cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg atacgtatca atttattaat
     3901 tgtttcaacc atatgaactg gaatacggat cgtacgtgct tggtcagcga ttgctcgagt
     3961 gatggcttga cgaatccacc atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc
     4021 aaatttttca actgccttca tcagtcccat gtttccttct tggatcaagt caaggaactg
     4081 catgcctcgt cctacataac gtttagcgat acttactact aaacgaaggt ttgcttctgc
     4141 cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc aatgctaacg ctacttcttc
     4201 ttccgcagtt aaaagggaga cacggccaat ttctttcaga tacatacgaa ccgggtcatt
     4261 gatttttaca ccggttggcg cagaaagatc ttctgttttt gcttgttcag cttttttctc
     4321 agcactcttc aagctgtgaa ttgatggatc accattttca tccaccacac tgatacctgc
     4381 gtcttcaatc ttttggatca atttgtccat ttcatctgca ttcaaagtga atggtgtagc
     4441 aagctgattg gataaatcat catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa
     4501 agctgctact gctgcttcat acgttttttt atctgttcca tttgccataa aagaaggccc
     4561 cccttgagta gctatttggc ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc
     4621 aattcaagct caagctgttt gtttccagtt cgtctggctt cttgctgcat ttgttttttt
     4681 aatgttattt catcagctaa gccagatttg ctgatcacat ctagcaagtc ttggatttct
     4741 cgtggcgaac tttcttctgc caccggtaaa tattcgattt ccaccaccaa ccgccttaaa
     4801 ttttcatctt gcaaaaagtc taaaaactct gcaaggttga attcataatg ttgtgagaca
     4861 tatgcatcga ataaaaaata aacttctgca tatgcatcat gaataaactg aaattctcgt
     4921 tcttttaaaa tattacggac agaaacttct ttaaatgcac gatataatag aatctgctct
     4981 gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag tttcttgtct cgtaacctga
     5041 gtgtgttgac tttcctgagg ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc
     5101 agttgttttt gtatcgtctc tctgccgatc tgaaactctg ttgacagttg agatagatac
     5161 atatcttgtt cgattggtga aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat
     5221 tcgatcttgt ctttttcatt atctaaattt ttcccttgtt ttaaataacg tgatttaaaa
     5281 gtaaagactg tttcgcgccc atgagccact aattcttgga aagatgcttc tccatactta
     5341 cggagatatt catcaggatc aagcttttct ggaacgacta cgacagaaag tgctaatcga
     5401 ctgttttgcc gaagcaattc aatccctcga ttggttgctt caaaacctgc gttgtctccg
     5461 tcatagcaaa agaccaattc tttcgtcagc ttttccattt gttggatctg ttgagctgtt
     5521 agacttgtcc ccatagaagc taatccagtt ttgataccag cctgccatgc agcaatcaca
     5581 tccataaacc cttcgaacaa caaaatttct ccgtttttcc ggatatcgct tcttgcttta
     5641 tcaagattaa ataaaacttg tcttttattg aacagttctg tttctggact attcaagtat
     5701 ttgggctgat ctttttcctg ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc
     5761 gtctggcctt gagaattacg aagtggaaac atcactcttt gataaaaacg atcagacata
     5821 cggccatcat catgcgtgac aaacaatcca gagcgttcta acaattcatt tgaaattgat
     5881 tcattcttga atacttgctg aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat
     5941 tcttcaatta attctaaaga caacccacga ctttgcaaat attctaaagc tggctgtcca
     6001 gcttttgtat gcaatagcat gtgatgatag atttccgctg ctttttcatg agcagcaatc
     6061 agtttccccg tagcagattc aggattttgg acagctatag tacgctgacg ccattgatct
     6121 tctaacggta tctgttcgaa gtctgccact tttaccactg cttctggaaa acttagacct
     6181 tctagttctt gaagaaaagt aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa
     6241 atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc
     6301 cccaagtaat ttttaccaga ctttttcaac tggacatatt gtccaatcac ctcaacgata
     6361 ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa tccgttgagc cattttttca
     6421 ccccctgctc tattgatatc ttttgggttt aacagaagat atttcaggag aataaccggt
     6481 aaaaaatcgc actaactgaa atcagtgcga ttttaaagga cacaattata catgtttgat
     6541 tgtatcatat ttcattgctt tttcaagtat aacacatttc tatctcatta agcatcaatt
     6601 tgctactctt ttcagtaact gattttgaat atcagccttc cagtacaata ctcgaagaac
     6661 aatatttacg agcatgaata caaagaagta tcctacgctc tctcccaacc ctaagtttac
     6721 ttccaaaatt ccatggaaca gcattgcagc tacatatatc cctacgatcg tcgttactgc
     6781 taggaagatg cgcaatgggt tgaagggata acaagctttt acaactgcca tgcaactgat
     6841 cccaatcagc aagtaataca tcaaagtagt agtttcagca tttccccatg cgaatgtttt
     6901 tccaagtaga tacaccgcaa tgatattcac tactacaagc aaggcatttg gtaaagcatt
     6961 aataagtgct gtaggcaaat aacggtaagt gatttttctt ttatcttgtt caaaagataa
     7021 aaagaaggaa ggataccctt caatcgctaa atcaatcagc gtgatttgga tagggataaa
     7081 cggaaatcca attgctgtaa cggcacatat aatcgataaa ataaatgaat aaatcgtttt
     7141 gataaaaaag atgcctgaga ctttcgtcac attgttgacc actcggcgtc cttcaaacag
     7201 gacttctggc aatgtagtaa aatcagagtc caacaatacc agattcgcaa tctgacgtgt
     7261 tgctccatca ccttctgcca tagcaatgct gcaatctgct tctcttaacg ccaaaacatc
     7321 attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt tctttcaatt catttaccag
     7381 caatttcttt tgctgcggac tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg
     7441 cacttccatt tcttcagtaa tttgagaaag atcgacatac gcatcataat tcggtaatcc
     7501 tgcacgtcta gcgatattgg agacagtaaa aggattgtct ccagaaatca ctttcagatc
     7561 cacaccttcc tcatgaagat aagctagtgt ttcttgagcg ttttttcgga ttggatcatc
     7621 gatttcaaac agagcaaccg gctcaacaaa tgctggtttt tcttcgttca attgctgatt
     7681 tggagcgatc cccaacataa gtacgcgata gccattcgct tgggctgttt ttagactgtc
     7741 cggaagccga tcttcttcca ccaaacgttc aggcgctcct acgtacacga ctcccagttc
     7801 tggaaattcc atcgcgcccc atttgcgatc agaagaaaat gcgactttgt ttgtcacatc
     7861 ataccgatta gaaagctcaa aataatctct catagcccgc atagtgatgt tgttatcact
     7921 gctttctgcc agatacgtcc ccataatttc tgggatggct ttctcgtaca gcggatgtag
     7981 tgcttctacc cgctggatac tcattttccc ttcagtaatg gtccctgttt tatccaagca
     8041 taatgtatcg acatgggcca gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt
     8101 ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt attagcaata ccaaaccctt
     8161 tggaagcatg cctaacaaag ctgctgcaga tgcaataaca gaaattttga tagaagcatc
     8221 gcggatgaac agtgcttcca aaaatagtat gatccccaaa ggaataatca cgaaactagt
     8281 gaatttggat actttgcgaa tcgaatctac aagttcagaa cgaatagctt tgtgagtctt
     8341 tgcctcgcta gtcaagcgga cagcataatt atctgcacct actcggataa cttcagccat
     8401 gacagaaccg cttgtcaaaa agcttccaga aagcaattca gctccagttt ctttgacaat
     8461 caaatcagat tcgcctgtca aaagagcttc atttgcttct gcccgtccag atataacctg
     8521 taaatcggct ggtatctgtt ctcctgctcc gatttttacc aaatctccta ataccagctc
     8581 tttagtagaa atcgattgtt ctttaccgtc ccggatcact agtacttctg attgggaaac
     8641 aatcgataat ttccgtacaa gatttcttgc atgtatttct tgataaattc caataacgat
     8701 attgacgaca ataattgcta aatacgccat gttggaatac gcacctacaa ataacagaca
     8761 aatcccaata ccaaagttca gaaaattgaa taacgtcaat atattatcgc tgaatatttg
     8821 ttttgtactt tttgatgcat cttcctggta gtcattttgc tggccagaag ccattttctg
     8881 aactacctct tgctgtgtaa gtccattcat tttctttttc tccattttcc gccaactttc
     8941 aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt aaaaacaact tgtttttata
     9001 aaatttatag aaaaacacac gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa
     9061 tcacccgtgt gctgttttta ttatcaatct atctttactt atattgttcc tccaaacggc
     9121 tcatccacca acctagtgct gtaccggcta taaaaagaac aggacaagct agtaccccag
     9181 caactcccaa tcccgcgtaa tcggtaggaa tgatcataat cgttgaagca aaaactacac
     9241 ctaaaatgaa atggaatagc ttgctatatg cctttgagaa aatgtaatcc atgatttttg
     9301 acaatccaag aacacaaaga atcccaccaa ttccgatcgg cacaatcact tcaagtcgta
     9361 cttctttaaa tccgtcagcc atcgctttat acaatcccat atacaccaaa aaattagaag
     9421 ggctcaatcc tgggacgatc atacccaaac caatcaatcc gccggcaatc agccatgttc
     9481 caaaattctg atcgacttgc ccgtcaaata attgagcgcc gtataataaa aacaaataag
     9541 ccagaacaaa agtaacaacc atgatgatca catcagaagt atctcttcct tttttccctg
     9601 cttctttcca taacgcagga acagttccag cgatacatcc gacaaaaaac cataatatcg
     9661 ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca
     9721 ctccgcctag accaacaggc agaaaaaaca aaacattttc tttgaaattt tttgtaaggt
     9781 gggctaaaaa agaaatcagc cgctcataca tgccaaagac agctgctaat gcaccaccgc
     9841 tgactcctgg caagataaat cctgaaccga taaacattcc cttcacaaac cgtaacatcc
     9901 agtctttggt tgtgtacccc acttcttgat tttccatttt ttgccttttt tccactattt
     9961 gcgtactcct tctttcattt aattttattc atgattgaac atttcctgct attttactta
    10021 aagacttttt cataggtctc tattattttt tcattcattt tgacaatatc aggataaaac
    10081 atgttacctc cattatgata gagatagccg ccattgtaaa gcaaggactg gatccgccaa
    10141 tatcggtatt gtttcccttc ctcatttcct aaaagtgggc tcagcacatc tttagaatac
    10201 gcttctgcta attctactgt gttttctccg ccatgatttt ttacataggc aatataatct
    10261 ttcccaaaat tatatgcttg gacagctgtc cataaatcgc atccttgttt ctttgcttct
    10321 actatcattt ccgccaaata tttgacacct tgatcaatac tgtcagctgg attatcaatg
    10381 atctcagctt ctccatgcag actttccgag ctctgcatga gatctgtccc ttttcctttt
    10441 gattcggtca atattatcgc cttgatcaaa ttctcgtagt tttccatatg ataatttttt
    10501 gcagctgttg ttatttctat ttgatagctt tccactcgtt ttaccgaacg ataagttctt
    10561 cctcataaaa acaacaccgc accaataacc gtcagaatga tcatgtaaaa aagaatacgg
    10621 ataatagata atcgttttcg cttcatttca tcacctagtt attttgacat ctttttttat
    10681 cttactattt ctttatcttc ttggctattt cttccttgat ttctgtgtac gccttaagaa
    10741 aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat gatcgttgtt tttcagtaaa
    10801 aacaattgcc caatgactga tataatcgtt atactttaca cagtaggtga ttatgtgaaa
    10861 aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa tttactatga aaagagcggt
    10921 caaggcttcc ctctcttttt actgcatggc aatgacggca gcggccgctt tttttcagaa
    10981 caggtccctg tactcgaacg ctactatacc gtgtatctag tagatagccg aggacatggt
    11041 cgttcgacaa acgaagcatc tatgttgaat tttcagttaa tggccgagga tctgaatacg
    11101 atcatgttgc tggaaaaaat cgatcaggca gattttcttg gatttagtga tggagcaaat
    11161 ctagcactgg tatttgccag cagcttccct aaaaaagttc atcgattgat tttaaattct
    11221 ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag tcatcagtaa tattcattat
    11281 gcatgggtat ggttactctc tttatttcgc ccaagtcttc gcaaaaatct tttagttatc
    11341 aaacttttgc tgcatgacat cgggcttaca gaaaatgatt taaaaaaaat caactccccc
    11401 actttgatca tcgtgggaaa aaaagatgta atcaagctaa aacattcttt gtatatagct
    11461 aaaactatac caaaagcttc ctttgtgctt gtgaaagaac aaggacatga attagctaga
    11521 aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc tttctgaaac ataactatta
    11581 ggaatagaag gtgatttatt tgttaaaaaa taccatacaa tggctgaaaa accacctagg
    11641 actgttcaaa acaatctttt tgatttctgt cattgtcatt attgtcggtg aattgatgtc
    11701 catcggtaaa actctatcta tccagcaatt aggagaaacg tttgccacta tacctgtatg
    11761 gaaaagcgga ctaatgctat tgattggtct tatctctgtg ttacctatgg ttggttatga
    11821 tattattttg aatcggatgc ttgaacaaaa gcagaatcct cgttacctgt ttgagacaag
    11881 ctggctgatc aatacgatca acaatattgc cggtttcggc gggtttgtta gtgtggggct
    11941 tcgctctgaa ctatatggac aaaagaaaga gagcagaaac gtgatccaag cattatcgaa
    12001 agttttcttg tttttgatgg ctggactttc tatttacagt ctgctttctt ttttacttgt
    12061 agtcttcacg ccagttgctc cttttttgaa gcaatattgg atatggttga tcggcggcgg
    12121 tctatatttt cctgtcgtct ttttatttac tactttcaaa aagaaaggat tccttggtgg
    12181 tacctcactt aaaactcaag gacagctatt gctagtttca ttgttggaat ggaccggtgt
    12241 cctgtttagt ttcctttctg ttggttattt aatggaaatc cggattgatc tgtggcaaac
    12301 aattccatta ttcattgctg cttcagtgat aggaatcgtg tctatgattc caggagaaat
    12361 tgggagtttc gatgtaatga tgattatcgg gttgtccgct attggtgtgc caagagaaat
    12421 agtcgttgtg tggatcttgc tctatcgcct gttctactac atcattccgt ttttgattgg
    12481 aatcgtcttt ttcttcaaaa atatcggttc cacttttgat cagcgttatt ctggtatccc
    12541 taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt gtcttattat acttttctgg
    12601 aatcatgcta gtactttctg ccactatccc gcaagctttt acagagtttc gctggcttca
    12661 tagtttgaac ccattgaaat ttcattttat tatccaattt cctagtatac tattgggatt
    12721 tcttttaatc attatgggca gagggatcgc agcacgtgtg aaacgagcat atttgcctac
    12781 aatcttcttg attgttctag ctttacttta tgttttatta agtgatttta gtttcacacc
    12841 ggtggttttc ttgtctattc tgcttctgat cattcttgct tctaaaaacg aattatttcg
    12901 ggaacaattg atctattcat gggagtggcg tacgatcgat ggaatcatta ttggtgcact
    12961 gtccttgctt tacatcgtta ttggggtata caatctgccg gatttccctc atcggcgtca
    13021 ccattttatt tcctttttct tatttccttc tgaaaagatt tggttttcgg gattgttggc
    13081 catcatcgct gttagtttca tgatcgttct atttgttcat ttccttcaag gagaaaagaa
    13141 acagattggc gaagccttta atgaagaaaa ggccctgaag atccttacaa cttatggagg
    13201 aaactccgat agtcaattga tcttcttaaa agataagcgg atgtttgcct atgaaaaaga
    13261 cggtgaacca actgttcttc tccaattcgc ttgcttcaat aataaatgta ttgtaatggg
    13321 tgatccttct ggtaagaaag aagattttcc agaagcaatc gaagctttta tcgaagaaac
    13381 agatcgacta tgctatttac cagttttcta cgaaacaagc gaagagattg ttatgatttt
    13441 gcatgaattc ggctacgatt tcattaagat gggggaagaa gcttatgtag acctgaatag
    13501 ttttacaact tcagggaaaa aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg
    13561 agaaggattt acatttgatg tcttacaacc accattttct actgagcaaa tgagtacttt
    13621 caaaaatatc tctgataatt ggttgggttc acgaaaggaa aaagggtttt ctttaggctt
    13681 tttctctgaa gattatctac aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga
    13741 aattgtcgct tttgcaacga tcatgcctac ttacacgaac aataaagtag gaacgattga
    13801 tctgatgcga tatgattcta aaaaagcacc ttctggcagt atggatttct tgttcttgca
    13861 tttgtttgcc tatatgaaag aagaacatat ccaatggttc aatttaggga tggcccctct
    13921 ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa aggattgcat acttggttta
    13981 cgagtttggt tctcattttt attctttcca tggattaaga gaatataaaa gcaagtacgc
    14041 tacaaactgg gtctcacgtt atactctata ttctagagat agttggattg catatgttat
    14101 gattgctttg ctgattattg ataatgcgcc agttgagcag acacaaggta gaataagtgg
    14161 tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat accgatagat gaacactctc
    14221 atgatgaata aaagagactg aagtaaaacg aaagttcatg cttctacaat ctcttcttat
    14281 acaattgaag aacaaagagc ggtccaaaca gaaatcctgt ttggaccgct cttttatttt
    14341 aaacattttt cattaccaaa atattcgagt cgcgtttctt atgcttcgat ctctgtaacg
    14401 atacctgaac ctactgtccg tccgccttca cgaattgaga atgtcgttcc gttttcaaca
    14461 gcgattggat ggatcaagtc aacatcaatc gtcacgttgt caccaggcat gaccatttct
    14521 gtatcttctg gcaatgtgat tgttcctgta acatcagttg tgcggaagta gaactgtgga
    14581 cgatagttgt tgaagaatgg agtatgacgt ccaccttctt cttttgtcaa cacgtacact
    14641 tctgctttga attttgtatg tggagtaatg gaacctggtt tagcaagtac ttgtccacgt
    14701 tcgatatcgt cacgttggat cccccgtaac aacacgccta cgttatcccc agcttccccg
    14761 taatctaacg ttttacggaa catttctaca cctgttacaa ctgctttttg tgtttcaggt
    14821 ttgatcccta cgatctctac ctcatcaccg acacgaacag caccacgatc aatacgacct
    14881 gaggcaacag ttcctcgacc tgtaattgag aagacgtctt ccactggcaa tagtaatggt
    14941 ttatctgtat cacgttctgg tgttgggata tattcatcta ctgtatccat caattccata
    15001 atagcagctt cagcatctgg atcgccttgc aatgctttta atgcggaacc tttgatcaca
    15061 ggagtgtcat cgcctgggaa accatattcg ctcaataact cgcgaacttc catttctacc
    15121 aaatcgatca attcttcatc atcgactaaa tcaactttgt tcaagaaaac aatcaggtat
    15181 tttacaccaa cttggcgtga caataaaatg tgttcacgtg tttgaggcat cggaccgtca
    15241 gttgcagaaa caactaagat tgcaccatcc atttgtgctg cccctgtaat catgtttttc
    15301 acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat
    15361 tctacgtgtg cggtattgat cgtaatccca cgttcacgtt cttctggagc tgcatcaata
    15421 ctagcatagt cttgaggatt tgctaggcct tttttgccta atactgtagt gatcgcagca
    15481 gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta
    15541 cttctgtcat aatgttcttt tgccataatt tacaaacctc ctaaataata tagatcactg
    15601 aaaaatttca gctctcacct ttttattata gtcttttttt gactaaatca aaaagaatac
    15661 gtccgactta ctttttgtaa gattcgaact aagcttatta tacgtcaata ttggtcaaag
    15721 tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt accttttata aataaaaaaa
    15781 tgtgtttttt gagaaaaact tcaagatagt ccttaacttt cttttgaagc aataagaaca
    15841 aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact ttcaatagac gaaatcttct
    15901 atttcgttaa taataaggaa agaggtgaag tacatgaaaa aattaattgg cgtacttgtc
    15961 gttttaggca tatttctcgg cggaagtttt gcagcttatc attattttta cggcggcgaa
    16021 gcttattata caaaaatcac gacgaatggc gaaatgtcag ctacccaagc agataacgga
    16081 gagaaattca cgacttatac ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa
    16141 caggtgacgc ttcgcgaaga aagagaacat ccattaaaga tgaatgcgta tcttaaactc
    16201 aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct
    16261 aaaaatgcgg cagaaaaaat cgataattag taaatggtct taaaatttta ttagataaaa
    16321 agaggctggg acagaagcgt ttaactccaa gaaataagaa ggagttgctt ctgcttccgc
    16381 cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtct cacgctcttt
    16441 ttttgttata ttcgtctttt ccgatccttc tcttgacaca attcatttaa ctgatcactg
    16501 gtacttgaac acctaatatt ctagtcattt ttataaacga ttcaatactt catccacatt
    16561 tttgatcatc acggatatcg taccatccgg attattcaca aattcaatca aatctgggtt
    16621 gcgatataca tctactggta cgatcagttc gatcccgttt gatagcttga atttttgttt
    16681 tccaaatttt ttctcagaaa tttcttgtac ttcttttaca ggaggtgctt ctgggacaaa
    16741 tccttgctct tgtacttctt ctttaaaagc catttttgcc gtgatgtttt ccttgaagac
    16801 tttttccgca atcatctcat gatccaaccg acctttttct tctatcgtat catagacagc
    16861 ttctttcaaa tcagccatga tatcaaattc ttctgtctca aatttttttc caagatgctt
    16921 ggctactttt tggatcactt ttacattttc ttctaaagaa ggtgcaggaa cactttcgat
    16981 cactcttcca gaaaaataaa gctctttttt gccagaaaac tcatattttt tttcaattaa
    17041 ttcgtaggtc caatcagtca aattcacagc gaatgcttca tctgcttttt gtgttttcgc
    17101 ccctagaatt gctcgattca aaatcaagcg gttgtcgatc cctgcttcgt ctgcttcaac
    17161 aaagtgagtg taagcctcac gataattgac tttcaataaa gataagtaca tcactgtatc
    17221 taattcatac aagacaacaa atacatctgc actaggtgct tcttcacttt cttggtagat
    17281 atcgaaccac cgctgaacga ttgcttcact tgcttccaca aaattttctt gagcgatttt
    17341 aagtgattca acgatcatgc tttcttctgc tagttgtcca gtttttgttt gagcagaaga
    17401 gattttttgg atcttctttt gaagaaaatc acgaatatat tcttttgtca gatccagctc
    17461 tttttgtgaa aagactggag aaccagtttc tctgtcaata atatgcagaa ttgctttttt
    17521 taaatagata tccattttct cattcccttc tgtcttagtt tacggaaaaa aaatcaaaaa
    17581 taccagaatc aaaatcattt tcttttaaac aaaaatttca agagtagaaa caaaatggat
    17641 tctttttttc gaaaaatgcg ctacacttat agttgaacga ataaaaaagg agctgaaagt
    17701 atgaattcat taacaagcac ttatcgtcta tcaaatggtt acgaaatccc ggtagtagga
    17761 ttcggcacat ggcaaacacc tgatggggat gtcgctgttt cctctgttaa agaagcgctg
    17821 gctgcaggtt atcgccatat cgatactgcc caaggataca agaatgaaga aagcgtcggc
    17881 caagcgatta aggaaagtgg aattcctaga gaagagatat tcttgacaac aaaattatgg
    17941 aatgcaaacc acagctatga acttgtgatg tcttcttttg aagaatcatt gaagaagctc
    18001 caaacagatt atttagatct gtttttgatc cactggccaa accctgttgc ttttcgtgat
    18061 aattgggaac aagcaaatgc agatacatgg cgagcatttg aagaactgta tgaagcaggt
    18121 aaaatcaaag caatcggtgt gagcaatttc ttaccccacc atctagatac actagcaaaa
    18181 acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg caccaggcga attacagaca
    18241 actgtagttg agtatgcaaa aaaacatgat atgatcctcg aagcatatag tccattaggc
    18301 actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg ctgaagcaca tgacaagacg
    18361 attgcacaag ttgctttgcg ttggtcgctg caacacgaat tccttcctct tcctaagtca
    18421 gtgacaccaa gcagaatcaa agaaaatact gaattatttg attttgaatt aacagaagaa
    18481 gaaatgaaac aaatcgatca gttagatggt gtagtcggca aagcaaaaga cccagatact
    18541 acccaatttt aagtcgaaag gtgattttat ggatccaatc gaacgcttga attctctctc
    18601 ggaagaagtt acccagactt ttcattccga ttttgtcttt ttgatcgatg cagagaaaat
    18661 ccaacatttt cctgcaagaa actggacaca tgatcaaatc atcgaagaac tgaaaaaacg
    18721 gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat gaagtcattt attcacctga
    18781 actacctgtt tttgccttga taccgaaaaa atagaaagaa tggaacggat tatgtatata
    18841 tacataatcc gttccatttt ttatttttct ttcaaaaaac gggaaaatac gcaaattttt
    18901 ctgttgtaat ttgcgtatac gcaagttttt ttgcattact ccttttcatt tggctgtttt
    18961 tttcttataa tcgaaagggt atgagcgaag gagagaacat atgaagaaaa aacagaaaca
    19021 aaaattatta aatcaattcc gtccttcatt ggatggtgta agaggacaat tattccgttc
    19081 tctagaagaa gagagcttgc gacgttatgg tttgccgtta caagtcatgc ttgatcccaa
    19141 aaaccgtcaa atcttaacga tcggattagc tgggaaaaca aatgaagata cacggatcaa
    19201 cgtgccaatc gatgacaact tcaatactgt attaaagaga attcgttcag gagaaaacgg
    19261 gattttcgaa cgattccgtg acaatcttct tattgaaatc gtgtcttact ggaatgatca
    19321 acgattgaaa aacgatgaac caactgcgca agctgttgcg acaccagtta cagaagaaac
    19381 agtcagtgtg ccagaagaaa aaacggaaaa accaaaagcg gacacaactg aaaatacacg
    19441 ctcaactgat acgaatgccg ctttaagttt tgctgatttt tcaaaagccg ttgctgagta
    19501 tcctaaattt tacacagaac aaaatgaaca tgaagtagtt attaatgaaa aaaatgctga
    19561 tgaacctcgt aaattagcaa ctatttctat gactactgaa aatgagttcg ttatcgaaaa
    19621 agcactcgaa cggaaataca aagtcaaatt gactttgatt cctttgatcg aacaatttgc
    19681 agcgacagcg attactgatc gataaaaatt ctatttgtca taaaaggctg ggatttaaaa
    19741 tcccagcctt tttagtcata taataatttc tacagcaagt aaaaagaaat tccttctaac
    19801 atcaggctca aatatcagct tgacctgtcc aacaaatcac ctatgatcct acaatgttag
    19861 gcgacttctc ttctacctga agatatcttg gagttgcctg atttcttaag atgcctaacc
    19921 ttaactataa aaaattctaa taataaacat attttctgat agagaaaaaa g
//
LOCUS       DACDOA010000050        19507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDOA010000050 DACDOA010000000
VERSION     DACDOA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.050"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWI39_001621"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWI39_001621"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9208731.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IWI39_001622"
     CDS             complement(1173..1868)
                     /locus_tag="IWI39_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9208732.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IWI39_001623"
     CDS             complement(1881..2597)
                     /locus_tag="IWI39_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9208733.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="IWI39_001624"
     CDS             2927..3532
                     /locus_tag="IWI39_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9208734.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="IWI39_001625"
     CDS             3857..4180
                     /locus_tag="IWI39_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9208735.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="IWI39_001626"
     CDS             4362..5732
                     /locus_tag="IWI39_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9208736.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="IWI39_001627"
     CDS             complement(5862..7163)
                     /locus_tag="IWI39_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9208737.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="IWI39_001628"
     CDS             complement(7393..9384)
                     /locus_tag="IWI39_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9208738.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="IWI39_001629"
     CDS             complement(9528..9794)
                     /locus_tag="IWI39_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9208739.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IWI39_001630"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IWI39_001630"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9208740.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IWI39_001631"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IWI39_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9208741.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="IWI39_001632"
     CDS             13138..13701
                     /locus_tag="IWI39_001632"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9208742.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="IWI39_001633"
     CDS             complement(13954..14475)
                     /locus_tag="IWI39_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9208743.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="IWI39_001634"
     CDS             complement(14478..15320)
                     /locus_tag="IWI39_001634"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9208744.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="IWI39_001635"
     CDS             complement(15313..16038)
                     /locus_tag="IWI39_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9208745.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="IWI39_001636"
     CDS             16214..16510
                     /locus_tag="IWI39_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208746.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="IWI39_001637"
     CDS             17078..18670
                     /locus_tag="IWI39_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9208747.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="IWI39_001638"
     CDS             18683..19423
                     /locus_tag="IWI39_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9208748.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DACDOA010000051        18877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDOA010000051 DACDOA010000000
VERSION     DACDOA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.051"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..580)
                     /locus_tag="IWI39_001639"
     CDS             complement(59..580)
                     /locus_tag="IWI39_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9208749.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            779..4471
                     /gene="nifJ"
                     /locus_tag="IWI39_001640"
     CDS             779..4471
                     /gene="nifJ"
                     /locus_tag="IWI39_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9208750.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4660..5547
                     /locus_tag="IWI39_001641"
     CDS             4660..5547
                     /locus_tag="IWI39_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9208751.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5544..6713
                     /locus_tag="IWI39_001642"
     CDS             5544..6713
                     /locus_tag="IWI39_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208752.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6737..8092
                     /locus_tag="IWI39_001643"
     CDS             6737..8092
                     /locus_tag="IWI39_001643"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9208753.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8164..9969)
                     /gene="glmS"
                     /locus_tag="IWI39_001644"
     CDS             complement(8164..9969)
                     /gene="glmS"
                     /locus_tag="IWI39_001644"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9208754.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERFSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10378..10668
                     /locus_tag="IWI39_001645"
     CDS             10378..10668
                     /locus_tag="IWI39_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208755.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10681..11706
                     /gene="sppA"
                     /locus_tag="IWI39_001646"
     CDS             10681..11706
                     /gene="sppA"
                     /locus_tag="IWI39_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9208756.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11709..12368
                     /locus_tag="IWI39_001647"
     CDS             11709..12368
                     /locus_tag="IWI39_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9208757.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12525..13457
                     /locus_tag="IWI39_001648"
     CDS             12525..13457
                     /locus_tag="IWI39_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208758.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13611..14900
                     /locus_tag="IWI39_001649"
     CDS             13611..14900
                     /locus_tag="IWI39_001649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9208759.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15147..16397
                     /gene="clpX"
                     /locus_tag="IWI39_001650"
     CDS             15147..16397
                     /gene="clpX"
                     /locus_tag="IWI39_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9208760.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16480..17067
                     /locus_tag="IWI39_001651"
     CDS             16480..17067
                     /locus_tag="IWI39_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9208761.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            17432..18759
                     /locus_tag="IWI39_001652"
                     /pseudo
     CDS             17432..18759
                     /locus_tag="IWI39_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6167 a         3367 c         4067 g         5276 t
ORIGIN      
        1 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctcc ttattttttt
       61 attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca aagatttctt
      121 tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg ttgatgacag
      181 cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact gcaaaatccg
      241 ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt tgtttgatct
      301 ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg ttccctcgct
      361 gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc ccgctttgga
      421 ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac ggacaagcta
      481 agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg attccttttt
      541 gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac ctctttcgct
      601 tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt ctaaaaatta
      661 gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa aaaaatggca
      721 tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat gatgaatcat
      781 gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt atgcatttac
      841 cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat tagtagatca
      901 atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag tagaaatgca
      961 atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg cactgacaac
     1021 tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca agattgctgg
     1081 ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa atgcgttgaa
     1141 tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg ctatgttagc
     1201 tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg cttctattga
     1261 aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg agattcaaaa
     1321 aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga aattagcttc
     1381 atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga accagaatcc
     1441 agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat tacctgaaat
     1501 cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg acttggtgac
     1561 ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag ttgcacagac
     1621 gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt ttttgaatat
     1681 ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc atacagtaaa
     1741 aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac ctctcttatt
     1801 agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg gtggtcgcta
     1861 cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg atgaattgaa
     1921 aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg atgtgactta
     1981 tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat tccaggccaa
     2041 gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta tcaagattat
     2101 tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga aaaaatctgg
     2161 cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag cgtatcttgt
     2221 cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct atgacttggt
     2281 caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg acgaacaatt
     2341 agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca ttagattcta
     2401 tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga tcaatacggc
     2461 aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg tgcttccgct
     2521 gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg tagaaaaaaa
     2581 catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg
     2641 gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata tacaagaaat
     2701 acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac ttgtagcaaa
     2761 tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc ggggcgtcgc
     2821 tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt
     2881 ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg aagaagcgcc
     2941 agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt
     3001 ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa
     3061 agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc
     3121 aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct tgtctacaca
     3181 attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg aaacacctta
     3241 tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg
     3301 ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg aatgtggtca
     3361 agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt atggtatgct
     3421 tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag tattgccaaa
     3481 agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt ttgagagtga
     3541 aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac gatcaatttt
     3601 ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca gccaatggat
     3661 gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc atgtacttgc
     3721 cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca atactggcgg
     3781 tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta gtggaaagta
     3841 tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt atgtggcgca
     3901 aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag cagaaaagta
     3961 tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct tgcttggcgg
     4021 aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt ggtcattata
     4081 tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg actacaaaaa
     4141 acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt cctcgctaga
     4201 taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg acgctaaaac
     4261 acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga aacgacttga
     4321 accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga cggaccttga
     4381 agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta aaaaaatgga
     4441 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
     4501 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcttgaaaat
     4561 tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa acatatgata
     4621 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
     4681 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
     4741 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
     4801 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
     4861 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
     4921 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
     4981 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
     5041 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgttgatc
     5101 actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc tttagatgca
     5161 gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
     5221 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
     5281 agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
     5341 gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag catcacgtta
     5401 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt gcgtagagaa
     5461 ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
     5521 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
     5581 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
     5641 caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg ccgatcaaga
     5701 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
     5761 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
     5821 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg ctaaaaattc
     5881 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
     5941 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
     6001 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
     6061 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
     6121 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
     6181 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
     6241 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
     6301 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
     6361 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
     6421 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
     6481 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
     6541 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
     6601 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
     6661 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
     6721 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
     6781 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
     6841 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
     6901 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
     6961 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
     7021 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
     7081 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
     7141 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
     7201 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
     7261 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
     7321 gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg ggttaaatat
     7381 caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
     7441 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
     7501 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact tggcagaaaa
     7561 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
     7621 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
     7681 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
     7741 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
     7801 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
     7861 attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
     7921 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
     7981 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
     8041 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
     8101 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
     8161 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
     8221 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
     8281 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
     8341 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
     8401 accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga tcaaagcaat
     8461 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
     8521 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa tgtaaaaggc
     8581 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
     8641 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
     8701 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
     8761 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
     8821 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
     8881 gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc cactttgact
     8941 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
     9001 taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac ctgctaaccc
     9061 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
     9121 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
     9181 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
     9241 ctcgatatct gaagcatcta cttgtgcttc gaatgaaaaa cgttctacga tgtcacctgc
     9301 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
     9361 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
     9421 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
     9481 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
     9541 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
     9601 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
     9661 ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
     9721 gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac ctatatttcc
     9781 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
     9841 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
     9901 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
     9961 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    10021 ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt agagtgcagt
    10081 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    10141 cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct ggaaacattt
    10201 aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa ataaattagt
    10261 atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg tttttatcta
    10321 tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg agtaaaaatg
    10381 gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc caaattcgaa
    10441 gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg ggatagcatc
    10501 caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac ggtagaagat
    10561 gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac
    10621 acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga
    10681 atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt ggtttcgtta
    10741 gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg
    10801 ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag tacgcaaaaa
    10861 atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg tttatttgct
    10921 tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga agaagatcca
    10981 acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg
    11041 gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat gtatgtcagc
    11101 atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga taaaatcttt
    11161 gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt gaattattca
    11221 ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc gttgaaagat
    11281 atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca agcatatatc
    11341 gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa atctgaagaa
    11401 gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa agaagtaggt
    11461 ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa ggaacaacag
    11521 cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc tagtacatgg
    11581 ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag tcagatctta
    11641 tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta ttacggaggt
    11701 gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc
    11761 agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct tttgaaagag
    11821 agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag tatacggaca
    11881 caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca ggttttttta
    11941 tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg cgtttaacac
    12001 ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg agtctctacg
    12061 gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa ttcaatcatg
    12121 gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat gaagaagaac
    12181 ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta aaatttcctt
    12241 tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat ctgggcgatt
    12301 ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac caggaagtca
    12361 acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa gtcagatcta
    12421 aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt tttgttttgc
    12481 tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag ccgattgatt
    12541 ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca aaccagcaat
    12601 atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc tttgaggcta
    12661 atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca ttgaagcaag
    12721 cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt tattttgact
    12781 tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt acagattttt
    12841 atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa ttatccggac
    12901 aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt ccttcgttgg
    12961 aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat catgagttta
    13021 tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca agggcaaaat
    13081 tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg acgattgaag
    13141 aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc
    13201 agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt agttccgtgt
    13261 taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat acatacatcg
    13321 aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa acgatgtatg
    13381 aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt ttgcagcaaa
    13441 tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga aaaacaagga
    13501 ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta aaggtatgca
    13561 tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa atgactgcaa
    13621 catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg caagacgaaa
    13681 tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac gtacctggat
    13741 tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa gaagctttat
    13801 atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc aaagaagctg
    13861 gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa ggtcaagact
    13921 gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa tataaagatc
    13981 tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca cgtatcaaac
    14041 gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct gaaaatggcg
    14101 atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat ggcggaaaag
    14161 gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt gaagatcaat
    14221 tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca gaagattatc
    14281 aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat gaagtaaaag
    14341 caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac gaagtatcaa
    14401 cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa gaaaaagcag
    14461 cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca gaaatcgttg
    14521 aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa ttcttgaaca
    14581 acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt tctacagaag
    14641 aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca aatcttgtga
    14701 tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc aatgcagaaa
    14761 tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc gtgttgacag
    14821 aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc acaggtacag
    14881 cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag ctacttctag
    14941 aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc cttatttttt
    15001 gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac actttttctt
    15061 tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt tcttgcatac
    15121 tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat gagaccgtac
    15181 gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct ggtccagggg
    15241 tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa gaattttatg
    15301 aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt gaagtattga
    15361 ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc gtatacaatc
    15421 actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa ctgcaaaaaa
    15481 gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca caaacattag
    15541 caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca gaagctggtt
    15601 atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct gattacaatg
    15661 tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt gcgcgtaaaa
    15721 gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa gccttgctga
    15781 aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa catccacatc
    15841 aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt gcatttgatg
    15901 gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt ggtacacaaa
    15961 accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa gatttactaa
    16021 aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct ttagagaaat
    16081 tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta gtcaaacaat
    16141 atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa gcgttgagag
    16201 ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt tccatcattg
    16261 aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc gaaaaagtga
    16321 ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat aaagattcag
    16381 agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct tttgcgacag
    16441 ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca tcaagctgaa
    16501 attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc agaaatagct
    16561 ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat tgatcgaaag
    16621 aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt ttatttgatc
    16681 gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt atccaaaact
    16741 gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga ccagctacgt
    16801 gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat ccagatgtat
    16861 caatttttga agtattatga tatccctgta attgttgttg ccactaaggc agataagatc
    16921 cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga ttttgatcgc
    16981 aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga agcgtgggca
    17041 gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag tcaaactctt
    17101 tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt ttcttgtctt
    17161 ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct tttggtgcaa
    17221 ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta tctgtacgtt
    17281 caaatttcaa ccccattgta aacacctctt tgtttctttt tacttagtct atcatgtaat
    17341 cgtccaaatt ggtttctaag acataaagtt ttagtttttc tttatcattg gttataatta
    17401 aattataaaa agatttgaaa gaggtgagaa aatgattggc gaagataaat tattgatgac
    17461 tatccagctc acagacttaa atgaagaaga aaaaaacagg aaataagcga ttgggctatt
    17521 ttgacacgtc tgttgatcca gccaacattt atccgctctt tcacacaaca agcggatccg
    17581 tatgatcttc gaaagcttca agaaaaaata atgctagcat cggtaagaga cgaaagttgg
    17641 ttagttgtag gaaatgacga gaacgaatgt agttttcgct tggaagataa tcaattattg
    17701 ataaaaaatg tattgtctat ctctgttttc aaagaaaagc aatttttgat tcgagacttc
    17761 attcagaaaa aaatgattgc tcatggggtg ttcgcctata tgcgagctta tagcgagttt
    17821 atctatcaca atacaaaaca gatctctcaa cggctattgt ttgagaaaaa ggatgaaatc
    17881 gaacatttac ctaaaatgaa acaacaaaac ggggaagtag tagtcgattg caaccaattt
    17941 ccaggatacg atctttttta ccaaggttta tgtttcactt cgtgttggga aatgtattat
    18001 agtcgctatt atcatcaaat catccctaaa ccgatttttc tcgacatcca acaagtagaa
    18061 aaagtgaagg aattagaaaa cgaagtcata tgtatccagc tttatcgaga cccctttaac
    18121 tggcaaaagc caaacaatca aatgtttcaa tcctatttta gggaccagct tgggtttgat
    18181 catcttgctt gggacaatgg tgtcggttta ttgaaaccgc cattcgttga atacatttac
    18241 actgaccata cgatccaatc tgtccagtat cagaatgcac aaatgcaacc tgttcctaaa
    18301 aagaatgctt cattttttgt aacaaaaagt tatgatatcc aacaaggaga ttataaagaa
    18361 aggcgtgttc gcggaacact aaatgctcag gcgtattttc cttgggttga cgagaatcga
    18421 tcacgcatga tgtgctataa agttatcgat ccgactgttg ctttggataa tggaatcgaa
    18481 gcttattgtt attatattag ggaatatcta gaagtagagg taacagatga aaaatatcaa
    18541 gagtatcttt tatccttaag aatctatgtt ccttcagaat ccctgtcaga attaccgtta
    18601 aaggaaatca aacatagact ctctgatgtt accttcaaac gaataaagaa aaagcggcga
    18661 agtatccaat tcgatgtcaa gaaaggtgag cagcatctgc gtgtgcaatt tttagattac
    18721 cgagaattag aacaattgat tactttacaa aaaatatgag gtgaagaaaa tggataaaca
    18781 actctattat cgaataatgg ttccggcatc attattatcc gtctgtctct tttttgttcc
    18841 taattttcct attcgatcag tcatcggttt tttggtc
//
LOCUS       DACDOA010000052        16572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDOA010000052 DACDOA010000000
VERSION     DACDOA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.052"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IWI39_001653"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IWI39_001653"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IWI39_001654"
     CDS             complement(227..697)
                     /locus_tag="IWI39_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9208762.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IWI39_001655"
     CDS             complement(856..1314)
                     /locus_tag="IWI39_001655"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9208763.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IWI39_001656"
     CDS             complement(1485..2630)
                     /locus_tag="IWI39_001656"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9208764.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IWI39_001657"
     CDS             complement(2643..3560)
                     /locus_tag="IWI39_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9208765.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IWI39_001658"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IWI39_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9208766.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IWI39_001659"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IWI39_001659"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9208767.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IWI39_001660"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IWI39_001660"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9208768.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IWI39_001661"
     CDS             7086..7967
                     /locus_tag="IWI39_001661"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9208769.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IWI39_001662"
     CDS             7964..8680
                     /locus_tag="IWI39_001662"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9208770.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IWI39_001663"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IWI39_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9208771.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IWI39_001664"
     CDS             9397..10398
                     /locus_tag="IWI39_001664"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9208772.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IWI39_001665"
     CDS             10395..11756
                     /locus_tag="IWI39_001665"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9208773.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IWI39_001666"
     CDS             11716..12867
                     /locus_tag="IWI39_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9208774.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IWI39_001667"
     CDS             12842..14011
                     /locus_tag="IWI39_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9208775.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IWI39_001668"
     CDS             14186..15565
                     /locus_tag="IWI39_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9208776.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16572
                     /locus_tag="IWI39_001669"
                     /pseudo
     CDS             15846..16572
                     /locus_tag="IWI39_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5424 a         2928 c         3525 g         4695 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagttggag
    16081 cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat cagaaaatca
    16141 cgacggatac aactgaattt aaataccatg aagtggatgc caaaggcatt atccgtcaaa
    16201 agaaattgta tctagatcct tttatggata tgttcaatag tgagatttta tcctatcgta
    16261 tctctgaaaa gcccaatgca ttagcggtaa tggaaggttt ggaggaagct atccagacca
    16321 ctaacgattg tccctatcgt cgtacgtttc actcagatca aggttgggcg tatcaaatga
    16381 aagcttatag aaacaaattg aaggagtaca aaatctttca aagcatgtct cggaaaggga
    16441 actgcttaga taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc
    16501 acgggaaagt ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt
    16561 actacaataa tg
//
LOCUS       DACDOA010000053        16470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDOA010000053 DACDOA010000000
VERSION     DACDOA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.053"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..203)
                     /locus_tag="IWI39_001670"
     CDS             complement(6..203)
                     /locus_tag="IWI39_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9208777.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(597..2324)
                     /locus_tag="IWI39_001671"
     CDS             complement(597..2324)
                     /locus_tag="IWI39_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9208778.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2317..3510)
                     /locus_tag="IWI39_001672"
     CDS             complement(2317..3510)
                     /locus_tag="IWI39_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9208779.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3697..4341)
                     /locus_tag="IWI39_001673"
     CDS             complement(3697..4341)
                     /locus_tag="IWI39_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9208780.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4435..4569)
                     /locus_tag="IWI39_001674"
     CDS             complement(4435..4569)
                     /locus_tag="IWI39_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9208781.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4571..6703)
                     /gene="feoB"
                     /locus_tag="IWI39_001675"
     CDS             complement(4571..6703)
                     /gene="feoB"
                     /locus_tag="IWI39_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9208782.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6700..7173)
                     /locus_tag="IWI39_001676"
     CDS             complement(6700..7173)
                     /locus_tag="IWI39_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9208783.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7574..7798
                     /gene="nrdH"
                     /locus_tag="IWI39_001677"
     CDS             7574..7798
                     /gene="nrdH"
                     /locus_tag="IWI39_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9208784.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7957..10116
                     /gene="nrdE"
                     /locus_tag="IWI39_001678"
     CDS             7957..10116
                     /gene="nrdE"
                     /locus_tag="IWI39_001678"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9208785.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10166..11131
                     /gene="nrdF"
                     /locus_tag="IWI39_001679"
     CDS             10166..11131
                     /gene="nrdF"
                     /locus_tag="IWI39_001679"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9208786.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11220..12359)
                     /locus_tag="IWI39_001680"
     CDS             complement(11220..12359)
                     /locus_tag="IWI39_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9208787.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12668..13381)
                     /locus_tag="IWI39_001681"
     CDS             complement(12668..13381)
                     /locus_tag="IWI39_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9208788.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13635..14336)
                     /gene="nagB"
                     /locus_tag="IWI39_001682"
     CDS             complement(13635..14336)
                     /gene="nagB"
                     /locus_tag="IWI39_001682"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9208789.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14570..16102
                     /locus_tag="IWI39_001683"
     CDS             14570..16102
                     /locus_tag="IWI39_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9208790.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16293..>16470)
                     /locus_tag="IWI39_001684"
     CDS             complement(16293..>16470)
                     /locus_tag="IWI39_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208791.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5107 a         3407 c         2860 g         5096 t
ORIGIN      
        1 tcattttagc acttccctgg aattgctcca cctaaaaaac cacctataga cattcctgca
       61 atacaagtag ttgccttggc ccaatcgacc gtacatttat ttttagtgca atacactcca
      121 tttccataat attttccact atgagtggta cccccataga taagttgtgt ctctttaata
      181 gacaaaattt ttaaatgttt cataatctaa tcacccctaa attaatgttt ttagactatt
      241 attctaacat tcattaaatt tcaaaaaaca attccttctt gaaacgtagc aaaataattc
      301 ctgaataaga atttaatcac ttttaagtat ataaactatg tatattcttt aatatttttt
      361 gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac ctacgatcga
      421 tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttga acgtttcgaa
      481 gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac atggagcttc
      541 caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct gtctttttat
      601 tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc tgcttgctgt
      661 ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc tgaccaatgg
      721 aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt tcgaaccatc
      781 gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta tttcagatca
      841 tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc gtgttttttg
      901 atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac tttccagtct
      961 ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc agctcctggc
     1021 tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga atttacatcc
     1081 atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc taggtcttcg
     1141 acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca agctgaaacc
     1201 atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc taatggcgtc
     1261 atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt tgaagcaact
     1321 tctgtatccc attcaacata agataagttg atacgttttg tttctttggc agatgaaaaa
     1381 ctccctatac caatcgcacc taagatcact actactacag caccaatgat cagtccttga
     1441 cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg ctggttcaaa
     1501 tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc gttgacaaat
     1561 ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa gccgggtgca
     1621 ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg attgatccct
     1681 gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg acgacttgtg
     1741 ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat ccctaaattc
     1801 gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc tggaaccatc
     1861 ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat tgtctgcata
     1921 aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat caagatccct
     1981 aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt gacagtattc
     2041 atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa tgtgaacaac
     2101 gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa caagataaac
     2161 aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc ctgtccaatc
     2221 gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt gatattttct
     2281 acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac tcacctcgct
     2341 tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta attttccatc
     2401 ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat cacttaccac
     2461 catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg gacgtttttg
     2521 cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc tctttctgtc
     2581 aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa cgtttggtcc
     2641 atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg taagcacttt
     2701 ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat tggtcaatat
     2761 ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga ttctgtcccc
     2821 aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt gaacattggc
     2881 ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa gagccgagaa
     2941 cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta ggccaacccg
     3001 ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa gcaaactaga
     3061 attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac cacggacttc
     3121 taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga agttttggaa
     3181 aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta acttcgatac
     3241 atcttctcca tcaatataga tactgcccga agttggctca atcagacgat tcaatagacg
     3301 gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt ctccttcttt
     3361 tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta aaatctccgt
     3421 tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac caaatatctt
     3481 tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg taaattcatt
     3541 tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt tgtcaaaaat
     3601 gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta aataaaaggt
     3661 ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct gatgctccgg
     3721 gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat gaacgatgtc
     3781 ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat tatgagaaat
     3841 tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg aacggtccag
     3901 atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca tcttatgtat
     3961 tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca gctcatcttg
     4021 ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt gtacatcttg
     4081 atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc cacttccatg
     4141 ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg tagtctcagg
     4201 agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac ccactttata
     4261 tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc gcgggcggac
     4321 gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc ctcatcttac
     4381 tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga ctgatcatcg
     4441 cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat tcttaccgga
     4501 ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac ttaataagaa
     4561 tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg ttcttggatt
     4621 ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca agatacgtcc
     4681 ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca aaactaacta
     4741 aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg gcattcattt
     4801 cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg aaggctaaca
     4861 atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt tctctgccat
     4921 tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata ttttctttag
     4981 cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca agcggctgaa
     5041 aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca gaaacaggtt
     5101 gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca aagataatcg
     5161 ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg accgctgacc
     5221 attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct ccagcaaaca
     5281 aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca agaaaatagg
     5341 cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag gcaataatcg
     5401 gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga cgatcttgct
     5461 cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta gcaatcagca
     5521 taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc atgacaaaag
     5581 cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac aatataatca
     5641 attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc aagatcagct
     5701 cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt tctggtaacc
     5761 atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact gtttgaatag
     5821 ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac caacgattcg
     5881 ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt gacacaacac
     5941 agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc gtctcactat
     6001 cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt tgctgagaaa
     6061 ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc attgcatacc
     6121 agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa gctgcttcta
     6181 aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca gactcctgaa
     6241 tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt aaaactggta
     6301 caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt ttgatgacat
     6361 ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc gtagttaaat
     6421 aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca ggctgttcct
     6481 gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa gaatagatgc
     6541 cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct tttttttcta
     6601 ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga ttgaacaagc
     6661 tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt cctccctttg
     6721 tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc gcactgtcag
     6781 ttcgattgga tctcccagag gggctagttt tcggataact atttttacat ttcgcgtcaa
     6841 tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta cgacgatccc
     6901 agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag cagtcttttt
     6961 attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac tttttaccag
     7021 taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg caacgatccc
     7081 gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga cttgataaac
     7141 gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt gaaataaatc
     7201 atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca aataaatgag
     7261 aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact tgtgatatta
     7321 tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt catagttttc
     7381 cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt tcagctcttt
     7441 tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt agtgtttgtt
     7501 gtttgacttt tttactaaga gttttatact atctattata gataataaga atgtgttaga
     7561 ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc aatgtaagat
     7621 ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata tcgatgaaca
     7681 gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg tcattacttc
     7741 tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg ccagctaaca
     7801 tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag caccagtttt
     7861 tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat ttgcaatcaa
     7921 gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa tttacaagat
     7981 gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca aatccctttg
     8041 aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc aaatacgatg
     8101 acttttccat ctattatgga aaaaatcaat tacttgatcg atcaagaata tatagaagca
     8161 gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt tttggacgag
     8221 caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca atatgcgttg
     8281 aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc cttcaatgcg
     8341 ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga aatgatccat
     8401 caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg tcggggcgaa
     8461 ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg gcgctcaatc
     8521 aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt atcaaatctt
     8581 cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt cgttccagtc
     8641 atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg tcaaggagct
     8701 ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc agcaaaaaaa
     8761 gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat cgtacctgat
     8821 aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc atatagtgtc
     8881 gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta tgatcatcta
     8941 gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga aaatgaaatc
     9001 tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac agcgaaccgc
     9061 gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat tttacaagtt
     9121 caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac agatatctca
     9181 tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt tggtcaatct
     9241 gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat cgatgtcgta
     9301 ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc catgggctta
     9361 cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt agactttaca
     9421 aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa tatcgctcgt
     9481 gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg cacttatttc
     9541 gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc attgtttgaa
     9601 ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt aatgaaagat
     9661 ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc atatatcaac
     9721 gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca agagaagaaa
     9781 atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc gtattacact
     9841 tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaacggc acaacaacac
     9901 gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga aggcatttat
     9961 gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag acattacgct
    10021 ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga tgcagaagaa
    10081 atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat tcgcttattc
    10141 atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa taaactggaa
    10201 tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc aattttggct
    10261 agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt ctcaattgga
    10321 aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg tccaatctga
    10381 aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag ccgttttgaa
    10441 caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt ttagtacact
    10501 gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc cttatctgca
    10561 aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag aaaagaaaat
    10621 tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc ctctttatta
    10681 tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca tccgagatga
    10741 atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg agttacctga
    10801 agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc tttacgaaaa
    10861 cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag aagtcaagac
    10921 attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc cactctttcc
    10981 agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag gcacaagcaa
    11041 ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag aagcgatgaa
    11101 agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa aatttttcga
    11161 aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct
    11221 tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt
    11281 cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca
    11341 ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag
    11401 atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt
    11461 ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag
    11521 ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata
    11581 atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc
    11641 attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact gctgatatag
    11701 gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc
    11761 ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa
    11821 ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag
    11881 acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct
    11941 gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt
    12001 aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt
    12061 tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa
    12121 aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt
    12181 ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaat tgccgaaagc
    12241 attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc
    12301 gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata
    12361 catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg
    12421 agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt
    12481 acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca
    12541 aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa
    12601 ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc
    12661 agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca
    12721 actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt
    12781 tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat
    12841 ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga
    12901 tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag
    12961 caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta
    13021 atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga
    13081 cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg
    13141 cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac
    13201 cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat
    13261 gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa
    13321 atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca
    13381 tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat
    13441 ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat
    13501 aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc
    13561 tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag
    13621 cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg
    13681 tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct
    13741 ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata
    13801 attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat
    13861 cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat
    13921 gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata
    13981 tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct
    14041 gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc
    14101 atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt
    14161 acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact
    14221 ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg
    14281 atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg
    14341 attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata
    14401 attagtatag accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt
    14461 aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt
    14521 cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact
    14581 gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct
    14641 gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac
    14701 attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa
    14761 aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga
    14821 agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct
    14881 ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca
    14941 aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag
    15001 cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca
    15061 acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga
    15121 tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga
    15181 ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac
    15241 ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc
    15301 attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa
    15361 acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat
    15421 ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc
    15481 aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac
    15541 aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg
    15601 ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa
    15661 aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa
    15721 aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata
    15781 tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc
    15841 agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac
    15901 taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc
    15961 gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc
    16021 aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt
    16081 cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca
    16141 aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta
    16201 ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg
    16261 atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt
    16321 cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc
    16381 gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca
    16441 cctccaataa agtacataaa cataaataca
//
LOCUS       DACDOA010000054        15917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDOA010000054 DACDOA010000000
VERSION     DACDOA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.054"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            236..904
                     /locus_tag="IWI39_001685"
     CDS             236..904
                     /locus_tag="IWI39_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9208792.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1104..1334)
                     /locus_tag="IWI39_001686"
     CDS             complement(1104..1334)
                     /locus_tag="IWI39_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208793.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1461..2843)
                     /locus_tag="IWI39_001687"
     CDS             complement(1461..2843)
                     /locus_tag="IWI39_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9208794.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2999..4186)
                     /locus_tag="IWI39_001688"
     CDS             complement(2999..4186)
                     /locus_tag="IWI39_001688"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9208795.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4193..5224)
                     /locus_tag="IWI39_001689"
     CDS             complement(4193..5224)
                     /locus_tag="IWI39_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9208796.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5270..6694)
                     /locus_tag="IWI39_001690"
     CDS             complement(5270..6694)
                     /locus_tag="IWI39_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208797.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6869..7774
                     /locus_tag="IWI39_001691"
     CDS             6869..7774
                     /locus_tag="IWI39_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9208798.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7865..8542
                     /locus_tag="IWI39_001692"
     CDS             7865..8542
                     /locus_tag="IWI39_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9208799.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8823..9719
                     /locus_tag="IWI39_001693"
     CDS             8823..9719
                     /locus_tag="IWI39_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9208800.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9774..11144)
                     /locus_tag="IWI39_001694"
     CDS             complement(9774..11144)
                     /locus_tag="IWI39_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9208801.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11382..12128
                     /locus_tag="IWI39_001695"
     CDS             11382..12128
                     /locus_tag="IWI39_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9208802.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12125..12964
                     /locus_tag="IWI39_001696"
     CDS             12125..12964
                     /locus_tag="IWI39_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9208803.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12965..13915
                     /locus_tag="IWI39_001697"
     CDS             12965..13915
                     /locus_tag="IWI39_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9208804.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14056..14808
                     /locus_tag="IWI39_001698"
     CDS             14056..14808
                     /locus_tag="IWI39_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208805.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14827..>15917
                     /gene="queG"
                     /locus_tag="IWI39_001699"
     CDS             14827..>15917
                     /gene="queG"
                     /locus_tag="IWI39_001699"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9208806.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKET"
BASE COUNT         5156 a         2989 c         3210 g         4562 t
ORIGIN      
        1 ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta
       61 gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac
      121 tacagtgtca cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc
      181 gtgttcgtga gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat
      241 tttttcaatt acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt
      301 gcttgggaag aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt
      361 aggtgggatc ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt
      421 tgccattgcg ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga
      481 aaaggtcaat cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa
      541 catgacagag ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca
      601 gcaagaatgc ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat
      661 gagtgtattg aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg
      721 gcaaattcag cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa
      781 cttgaagaaa gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga
      841 aaaagggctc tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg
      901 atagatagac agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt
      961 tttcacaacc tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag
     1021 ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag
     1081 ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca
     1141 atataccgta cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag
     1201 gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa
     1261 ttgagctgaa tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt
     1321 tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt
     1381 tgataccgtt gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc
     1441 gatctcgata ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct
     1501 tctaatccac tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt
     1561 cctccttgca agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc
     1621 gtatcgacaa caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg
     1681 ttaagctgta aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata
     1741 tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta
     1801 gcatttgtcg cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga
     1861 tagcctaaag agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc
     1921 aagcgagtgc cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt
     1981 gtataatctc tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct
     2041 tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc
     2101 cctagcttgc ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata
     2161 cccatttgtt tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct
     2221 gagattttcc ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt
     2281 ccgtcttttg agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct
     2341 atagtcgtgt agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg
     2401 tacgcatttg ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg
     2461 acggaataga catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct
     2521 ctgttctttt ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag
     2581 gggaagggaa gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca
     2641 ggactggaag ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac
     2701 atgactacta atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg
     2761 taaaaagatt tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat
     2821 accggatttt gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc
     2881 tctttccgtg tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga
     2941 aattgctgag aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc
     3001 aaacatgaac agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa
     3061 attccggagc atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag
     3121 gtatattttc ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc
     3181 gtgcacctaa gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc
     3241 cagttacttc ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg
     3301 cttctaagtt cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa
     3361 cggcatggcg agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa
     3421 attcttcttc atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca
     3481 aggcttcttc gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct
     3541 tattcgtatt catgatgacg attgcataac catctagttt cactggaacc atttcgtatt
     3601 tcagcgtatt tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact
     3661 gatccatgat acctgaattg acaccgatga attcattttc tactttttta cctgtttgga
     3721 ctagttccaa gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata
     3781 attctaatga agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata
     3841 actcaaaccc atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta
     3901 cgtaatttgc ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga
     3961 caccgatatc gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg
     4021 ctacaccttg cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg
     4081 tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc
     4141 cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct
     4201 ttcttgtttt gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa
     4261 tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc
     4321 caccgtgatg tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag
     4381 tgaaaataac cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc
     4441 tagtcaagcg agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga
     4501 ttccgtcaac aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag
     4561 gtatttgaaa atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat
     4621 ctgttcgcaa aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact
     4681 gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt
     4741 ggtcactggt gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga
     4801 tttccacatc cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa
     4861 tgacagaagc ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc
     4921 caggagcacc accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt
     4981 ttccattgag agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac
     5041 cgatatacgg atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc
     5101 gatcatttga ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct
     5161 tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct
     5221 tcattttcaa gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca
     5281 ttgtatcagc tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct
     5341 ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag
     5401 catagaatgc ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag
     5461 cattaccagt catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac
     5521 cagcgatttt atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat
     5581 ttttccattg accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca
     5641 attgttgagg gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg
     5701 cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc
     5761 gacgagagat gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg
     5821 cagcaactag accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt
     5881 agaataatac ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa
     5941 gccacatcaa ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt
     6001 cattttgctg acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag
     6061 taccgcctga aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa
     6121 tatgtttatg agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag
     6181 taccatttgc acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct
     6241 ttgtttccgt actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa
     6301 aactatccaa taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag
     6361 tagctaagcc aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc
     6421 cgataactaa ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac
     6481 caatcaaagg atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg
     6541 tagcaatcat ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga
     6601 ataccataaa ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag
     6661 cgtaggacac acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata
     6721 tataatacta agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta
     6781 acaagaaatc gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat
     6841 tatactaaag gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca
     6901 tcaacaagta gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt
     6961 tcattctttt ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa
     7021 aggatcttat catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat
     7081 tcgtccagga gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg
     7141 gctttcattt ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga
     7201 ccaatacacg atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat
     7261 gaaagatacg gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt
     7321 tttatcagtc cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc
     7381 ctctttagct gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt
     7441 tgaggaaatc gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa
     7501 tcatatgggt accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt
     7561 tcttctttct ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag
     7621 tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg
     7681 taaacgtatg aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt
     7741 ggaaaaacaa accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc
     7801 ggcaaaagaa acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa
     7861 agggatgcgt acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca
     7921 atttttcaaa gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta
     7981 tccagacgga aaaatagata tagagactga actacagctc ttggcccagc atgatcggat
     8041 cattttccag tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga
     8101 taatgtcttg gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac
     8161 aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga
     8221 atttttgagg ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt
     8281 cgttttagca caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta
     8341 tcagcagtat ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat
     8401 tcaaagagta caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat
     8461 cgtagaagca ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga
     8521 aatgaaagga acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc
     8581 gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag
     8641 atagacgaac gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc
     8701 aactggtgaa attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg
     8761 ctacaataag aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa
     8821 aaatgtatga tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa
     8881 aacaggcagc tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca
     8941 tagcaggagg agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta
     9001 cgaccataac ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa
     9061 ctaaaacaaa agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac
     9121 ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg
     9181 aagaatacca gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg
     9241 ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg
     9301 cagcgtatca ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat
     9361 tatcggctaa ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg
     9421 cctctcttca agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag
     9481 cagagtcggt agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct
     9541 ctgaaactaa taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag
     9601 aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga
     9661 atgggatgag catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat
     9721 aaaagcaata aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt
     9781 ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca
     9841 ataatcaatg agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa
     9901 aaagcaagtg tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc
     9961 attttatctg cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga
    10021 accataccag gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg
    10081 atgaacattg gtagaagata atttaagatc agaccaatta agattccacc tgagattgct
    10141 acgacaggat aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt
    10201 ccatttatgg caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct
    10261 gataatgccg ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact
    10321 ttagaaaaag tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata
    10381 ctcacaatga taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg
    10441 acagcttcaa tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt
    10501 tcgattcctt gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct
    10561 ccagtgaaga atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta
    10621 ccaaatccaa ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact
    10681 ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc
    10741 agcataagaa acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa
    10801 taattcatat aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa
    10861 atattactcc aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt
    10921 gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa
    10981 tacaaaaaga atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg
    11041 gttgaggagg cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg
    11101 atatgacggg cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg
    11161 ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt
    11221 tagccttatt gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat
    11281 tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat
    11341 ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg
    11401 taattcaaga cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga
    11461 cgataaatcc acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat
    11521 ttatgaaatc tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac
    11581 agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc
    11641 tttcctttcc aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa
    11701 ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag
    11761 ggatggaaga atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag
    11821 tttttattgc aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg
    11881 tagggattga tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt
    11941 caggaaaaac gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg
    12001 aggttattct tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat
    12061 cgaagaacct tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga
    12121 aaaaatgatc caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt
    12181 ggtcacttcc atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg
    12241 agggatgtct ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc
    12301 ttatatgttt ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc
    12361 agtgattggt ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt
    12421 ctttagctcc ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga
    12481 tttgtatcat attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac
    12541 agcactcgtt agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat
    12601 cacatcattc gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta
    12661 tcttttgatg ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat
    12721 cttggtgatt gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt
    12781 actgaaaatg atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt
    12841 tttcagttat agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt
    12901 ttttatcctt gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag
    12961 atagatgaaa acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc
    13021 cggttgcgga aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt
    13081 cgtggctacc aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga
    13141 tatccacagt atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga
    13201 catcaaaaag gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg
    13261 taacagctgg ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt
    13321 tgcagttagc aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc
    13381 agatccccat gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg
    13441 tatattctct gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata
    13501 tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga
    13561 gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga
    13621 tttgccagca gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat
    13681 gaaagcgatc attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag
    13741 tgtagactca agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa
    13801 actgttcact gattccatag caaaagaagg agaacaagga gattcttact atcagatgat
    13861 gaaatggaat attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt
    13921 gcaaggtcta gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta
    13981 tgctgttttt cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg
    14041 aaggaaggtg tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc
    14101 tttcgattga tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa
    14161 gcagaattag ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag
    14221 ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc
    14281 ggttattttg tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac
    14341 gacgaaataa ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta
    14401 atcaaaaaga tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg
    14461 aatatgtttt ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc
    14521 acgtacacac agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag
    14581 ttttcgccat gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg
    14641 aattttttta atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta
    14701 tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt
    14761 gtccagctga aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg
    14821 aagaccatga cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa
    14881 atcgggttca ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa
    14941 gcagctggcc atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa
    15001 atgacatttg atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc
    15061 catgaagaag tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc
    15121 gattaccatg atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga
    15181 gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa
    15241 ctgatcgatg tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg
    15301 ttgatcactg aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta
    15361 tttgagcctg ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct
    15421 tgtccgacaa aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat
    15481 caaactcaga caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt
    15541 tatggatgtg atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt
    15601 catgaagaga tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg
    15661 atgtctaata aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag
    15721 aaacctttgc agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg
    15781 cctgagattt cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg
    15841 gctattggac gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata
    15901 ttagctaaag aaacaga
//
LOCUS       DACDOA010000055        15749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDOA010000055 DACDOA010000000
VERSION     DACDOA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.055"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IWI39_001700"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IWI39_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9208807.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IWI39_001701"
     CDS             complement(481..675)
                     /locus_tag="IWI39_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208808.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            758..1366
                     /locus_tag="IWI39_001702"
                     /pseudo
     CDS             758..1366
                     /locus_tag="IWI39_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1386..2414
                     /locus_tag="IWI39_001703"
     CDS             1386..2414
                     /locus_tag="IWI39_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9208809.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            2411..3169
                     /locus_tag="IWI39_001704"
     CDS             2411..3169
                     /locus_tag="IWI39_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9208810.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(3255..3995)
                     /locus_tag="IWI39_001705"
     CDS             complement(3255..3995)
                     /locus_tag="IWI39_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9208811.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4056..5309
                     /locus_tag="IWI39_001706"
     CDS             4056..5309
                     /locus_tag="IWI39_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9208812.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            5610..5858
                     /locus_tag="IWI39_001707"
                     /pseudo
     CDS             5610..5858
                     /locus_tag="IWI39_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5980..6306
                     /locus_tag="IWI39_001708"
     CDS             5980..6306
                     /locus_tag="IWI39_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9208813.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            6374..7087
                     /locus_tag="IWI39_001709"
     CDS             6374..7087
                     /locus_tag="IWI39_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9208814.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            7397..8249
                     /locus_tag="IWI39_001710"
                     /pseudo
     CDS             7397..8249
                     /locus_tag="IWI39_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            8370..10235
                     /locus_tag="IWI39_001711"
     CDS             8370..10235
                     /locus_tag="IWI39_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9208815.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            10283..11692
                     /locus_tag="IWI39_001712"
     CDS             10283..11692
                     /locus_tag="IWI39_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9208816.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11800..12501
                     /locus_tag="IWI39_001713"
     CDS             11800..12501
                     /locus_tag="IWI39_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9208817.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            12660..13139
                     /locus_tag="IWI39_001714"
                     /pseudo
     CDS             12660..13139
                     /locus_tag="IWI39_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13987..14562
                     /locus_tag="IWI39_001715"
     CDS             13987..14562
                     /locus_tag="IWI39_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9208818.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            14562..15002
                     /locus_tag="IWI39_001716"
     CDS             14562..15002
                     /locus_tag="IWI39_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9208819.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            15018..15719
                     /locus_tag="IWI39_001717"
     CDS             15018..15719
                     /locus_tag="IWI39_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9208820.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5290 a         2647 c         3291 g         4521 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttaccca gaaaatcaac tttttgcagg
      781 agatgtatta gaaattgtat taaacgacca agcaggagaa gctgctgcta agggtgtgca
      841 gaacaagcca agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt
      901 ttatcatgat gccccgcctt ttgcacctgc tacgaagatt gtagtagaag gtggtctctc
      961 atttcttgcc ccttccaaac tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga
     1021 agcatttggc caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacaaa
     1081 aagtccatgg atcttaacga taaaattatc tcagcctttc acaaccgatg caggattttc
     1141 tatgcaggat gtcctatatt tcaagaaagc tgaggagtat caattagtga atgaagaaga
     1201 actggaaatt gtcagaggac aaaattcaac gaatgtgcaa acagattatt cagaatattt
     1261 aaataatggt cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta
     1321 ctcagcagag atgacatgga cattagcaga tgcgccgaca gaataaagat caaggagaat
     1381 gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttctgatcc
     1441 ttctccttgg attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg
     1501 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
     1561 tggagcctgg gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag
     1621 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
     1681 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
     1741 cagtgggaga aataactgtc cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
     1801 ctccagaaaa agagctggct ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag
     1861 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
     1921 tcttgattcg aaatagcgaa aaagagtctt ccccaaattt gaaattaaaa aaaacttatg
     1981 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
     2041 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc
     2101 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
     2161 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
     2221 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
     2281 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
     2341 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
     2401 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
     2461 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     2521 tgtaacaaaa ccagatgccg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     2581 aaatgatccc gatacgccgt ttacacctaa cgaaccaaat cagggaacat ccggattact
     2641 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     2701 agtatatgct gcattacaaa acggaacgga cagtacaggg acagcaacac aagtacagaa
     2761 tttcgtacaa gtaacggata aaagcggaat ttatgccggt tggaaactat cagtcaaacg
     2821 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     2881 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaacgccat ctactatttc
     2941 tggtactggc gctgaaggaa ttgagatccc agcagatcag gcagtagatc tagtaactgc
     3001 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     3061 gagacaatca gtggaattat ttgttcctgt atccaattat gccgtagaca actacgtttc
     3121 aacattcaca tggtcgttaa cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa
     3181 tgacttcagg gagtggagcc gattcaaggc caactccctg aaatctgcta tgcttacgtc
     3241 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     3301 ttttcaaaga tctttcgacc aacgagtaag gagtcaccat tgaaaaattc taacacataa
     3361 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taatagtaaa
     3421 aagtaagcat acttttccaa ctctgtcttc aattgtttta aagaagtatt cactaattta
     3481 tcttctgtag ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat
     3541 aaaatttccc gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg
     3601 acatttacga tttgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca
     3661 gataaattcc tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct
     3721 actgcaaaat tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt
     3781 atttgttcgg cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa
     3841 acatcgtgat tatcaatagt acccaactgg atcaactgat tgatttgaga gatagaagtg
     3901 agctgcacga tattctcgat atgatgattg accaattctt tgatgatcca ttcgctgtga
     3961 aactggttat cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat
     4021 gtgtacattt tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt
     4081 tgacctttac tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag
     4141 taaaaaacag ctttggtttt atagctttga tggtggtgac acttttattt cctatcccta
     4201 aagtgagctt tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga
     4261 aaaacctgtt tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct
     4321 tttttctggt ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt
     4381 tttttgcact acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga
     4441 cgagatttct caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta
     4501 ttttattaag ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta
     4561 attatctgct tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca
     4621 ttatgttgat cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc
     4681 aaataaatga agaatattac catcatctag aggaattcaa acatgattat aaaagtctac
     4741 tgttttcatt aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa
     4801 tcttagaaga tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata
     4861 ttgtatcccc agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga
     4921 aaatcccgat ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg
     4981 tttttattcg ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc
     5041 agagtccgat tacgatcaat ctgctggaag aaaaagaggg attacgtttt agtatagcga
     5101 acaaggcaaa tccgacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta
     5161 aaaaaaatgg cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg
     5221 cagaactaaa aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg
     5281 catctagaaa gagcaaatca gcctactgac ttactgtcaa aggataagct tgaggaaagt
     5341 ctacacggag atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg
     5401 ttgtcaccct tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac
     5461 ttagtattag tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa
     5521 cccacgatcg tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc
     5581 gcttatttta ggcgtggaga agcaagaaaa tccctacgat cacacaaaaa atgctgaccc
     5641 agcagttaag agagttagaa caagatggca ttgtccatcg aaaagtctat aaccaagtcc
     5701 cgcctaaagt agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag
     5761 cgatgtctgt ttggggagaa gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca
     5821 cattactgca tacacatggt ggctacctaa attactgatt tctatgacat ttattttaaa
     5881 taaaagaaaa caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat
     5941 gattaatcaa gtaagaaatg attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt
     6001 aaccagttta gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatt
     6061 tctatttctt tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa
     6121 gtaaaagaaa cacaattaga acgaatcaaa gaagaactgg cggatgtatt gatctattca
     6181 tacatgatgg cagacaacct tcagctggat ctagatgaga tcattgaaga aaaattgatc
     6241 aaaaataatt tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag
     6301 ttttagaaat gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct
     6361 ggagggagag aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt
     6421 gttcctcttg ttattagtag gactagcttt gattttcaat gaacagatca aggattatct
     6481 tgtcagagag acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa
     6541 gaataacgat aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg
     6601 ggtcatgagg tcgcagatga gaggaacaga tctaccagtg attgctagta tcgcagtccc
     6661 ttcagtttca atcaatttac cgattttcaa aggcttggat aatacgtcac tactttacgg
     6721 agcaggaaca ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca
     6781 tcgtgcaacg aaccctgaat tgttgtttac accgctggaa aatttagaga tgggtgccaa
     6841 gatctatttg acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt
     6901 tgctcctaca gatacccaat tactgaatga agtcgaggaa aaagaaattg tcacactaat
     6961 tacttgtggc gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt
     7021 tacaccgata aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac
     7081 attttaggat actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg
     7141 ataaggaaaa accaatttgc tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg
     7201 gttgttttct agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca
     7261 taaataaaaa ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca
     7321 ggcaaaacca gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt
     7381 gagagggcga agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa
     7441 aaatggattc ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga
     7501 ttggggacga gatttcgcca gaagcggtcc aaaaagaatt tattttaaga gattcttttg
     7561 ctaaaaacca attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca
     7621 aacaagtagt cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc
     7681 tgacattgac cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc
     7741 ctaatccatt attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag
     7801 aggctctttt gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt
     7861 ttattgcctt tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga
     7921 ccgctaccac aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt
     7981 tcgatgaaga atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc
     8041 gttatctcaa aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag
     8101 gaaagtatcc aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc
     8161 aaaaacagga aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag
     8221 taagtgagaa aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg
     8281 attgaaaaaa taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt
     8341 ttaaataaaa aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat
     8401 gggaagcggt aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc
     8461 gattcagatt aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg
     8521 tgatccaagt cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag
     8581 atgtttatca atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag
     8641 agtccaaaaa tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc
     8701 taggggcgat ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg
     8761 gctggctgac agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct
     8821 ttaccttctt acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt
     8881 ttttagcagt agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg
     8941 cgggagaagc tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt
     9001 cttctgtcat accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa
     9061 aaaaagtgat tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga
     9121 tggttccatt aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag
     9181 gcggtctctt caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa
     9241 gtttatggca agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt
     9301 tgaatatcga acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg
     9361 cacaaggtgg ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat
     9421 tagggatttc ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg
     9481 tgacattacc tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag
     9541 ctattatcgg attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga
     9601 ttccgacttt cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag
     9661 ctacaggaat tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac
     9721 aaacacaaga caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag
     9781 cacggcatac cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag
     9841 accaagtttt ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag
     9901 aactagttgc acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag
     9961 ggatcacaac gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat
    10021 taaatgggaa tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt
    10081 tattgattcg ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag
    10141 ttgtcattac gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta
    10201 ttgcaaacga agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt
    10261 ttttcagaaa aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta
    10321 tgatcgagac ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt
    10381 tgcgattatc ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc
    10441 gaatcatcga ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga
    10501 aatgggattc aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga
    10561 cgaaggtact cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt
    10621 aaaatatgat attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc
    10681 aaaagaatac ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca
    10741 gacagtatta gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa
    10801 ttcggccttt cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga
    10861 gtaccaaaat attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa
    10921 aatcggccac gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac
    10981 ttacagcatc gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt
    11041 caattttttc tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac
    11101 ccataaaaaa tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt
    11161 gctaaaagaa tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa
    11221 tgagactgat ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc
    11281 gtttcttgag gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt
    11341 gaatgaatta tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc
    11401 cattgatcat atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg
    11461 tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac
    11521 gccttgggga tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg
    11581 ctttatctat gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa
    11641 atcttttgat tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca
    11701 gctgaatgaa aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg
    11761 tgctattctt ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta
    11821 tacttggaag tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt
    11881 tcgatgttgc cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc
    11941 cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg
    12001 acgacggttc taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa
    12061 atcttggaga actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat
    12121 ataaagaagg aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc
    12181 ttatattatt tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg
    12241 atgcaattca gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa
    12301 ggatatacta ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa
    12361 caaggaattc tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg
    12421 caaattgggg aaatgattga atattcggag gcggtctacc atacggagct gcatccttac
    12481 catattgatt atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag
    12541 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac
    12601 cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat
    12661 tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat
    12721 tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc
    12781 aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat
    12841 gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga
    12901 cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa
    12961 tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt
    13021 cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa
    13081 tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg
    13141 agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta
    13201 tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa
    13261 ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg
    13321 ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt
    13381 actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct
    13441 agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt
    13501 gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca
    13561 gctatttatc gttaacaaaa agatttttag gagaagctga tgaacaaaaa tgatcaaatt
    13621 ttgatcgttt agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata
    13681 acacgagaac aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata
    13741 atacacgtta ttggcatata ggacgaaatc ccagtatctt ctataagtaa gtcatttcat
    13801 atcgatgaaa acacgttatt tatttttgtt ttatctataa aatggctgaa aaatgatttt
    13861 tttctaaata ctatatatag tggcgtctat cctatatacc actaaaaaac tacaaaatct
    13921 agtcttttat tttaaaaaaa cacgtgtaga atgaagatag acttttgagt tagggagtgt
    13981 ttttttatga aacttactgt tttttgcggt tcccgttttg gtaataaaga aagttacaaa
    14041 tttattgcgc aaacgttagg aaaatacatg gcacaagaag aaatcgaact ggtttacggt
    14101 ggttcatact ccgggattat gggagtgatt agtaaaacag tactggaaaa taacggaaaa
    14161 gtcacaggta tctatccgaa tgggcttttt gaaactgaat tgcctggaaa agatgttact
    14221 acatttattc ctacagaaac gatcgatgaa agaaaggtat tattatttga aaagggagat
    14281 gctgtcctcg ttttccctgg aggtctaggt acattggagg aattctcaca acttctttct
    14341 tggatagcaa tcggtctgac gcccgacaag ccaatcggaa tattgaatat tggcggttat
    14401 tacagcggct tgcaacaatt attagaaact tttgcaaaag aggattttat ggataaaaaa
    14461 tggttggatc aagtaatctt ttccaacaat ccattggaac tggtcaatga attacgagca
    14521 gtatctatta aaaatcagtt attattgaag gaggcaaaat aatgagtcaa atatatttag
    14581 caggaccatt tttttcagag gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg
    14641 aggaaaacaa aacagtcaca agtttctact ctccacgtca tcaccaagaa agcaattatg
    14701 aactattcag cgctggatgg gcgcaggaag tatacgaaaa agatatggaa gaactgacaa
    14761 atgcagaatt tgttgttgct atccttgact ttgagcatca aacaatcgat ccaggaacgg
    14821 cttacgaatt aggtgtggca actatgttga aaaaaccgat gatcattgta caagaagaga
    14881 cggtacctac gaacttgatg attacacaaa gtctccatac gtatttaaaa tcagaccaag
    14941 ctgtacgtga atatgatttt gaaacattgc ctgttgaaac ttacgtaggc gagtacttat
    15001 agaagaggaa gggttgtatg tcagtgattt tgatttcagg aacgattggt gcaggtaaga
    15061 gcagtttgac ggatatgctt gcaaaagaga ttgattctaa accattttat gaaaatgtcg
    15121 aagataacga agtattgccg ctcttttatt caaatcctga gcagtacgct tttttattgc
    15181 aaatcttctt tttgaacaag cgatttttag caatgaaaaa tgctttagtg aacgatgaca
    15241 atgtgctaga ccgatccatc tatgaagata gcctgttgtt ccatctaaac gctgatttag
    15301 gtcgggtaac ggatatcgaa gtacagcagt acgatagctt attagatacg atgctaaacg
    15361 agttagatga tgttgctcca aaaaaacggc ctgatttgat ggtccatatc aaagtttctt
    15421 tggacacgat gctagaacgt atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg
    15481 atgagacgtt atatacctat tatgaaacgt tgaacacccg ctataatcaa tggtatgaag
    15541 attttgatgt atgtcctaaa atacaagtgg atggggataa attcgatttt gtagaaaatc
    15601 cagaagatgc aaaaaaggtg atccgtcaga tccaagaaaa attagctgaa ttggaaggaa
    15661 agcctgctgg aaaatatttt acggataacc agcatgcaaa aggtcaagaa ttaggctaaa
    15721 aataaaaaac caaacgacgg gaaagaaaa
//
LOCUS       DACDOA010000056        15134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDOA010000056 DACDOA010000000
VERSION     DACDOA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.056"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..317)
                     /locus_tag="IWI39_001718"
     CDS             complement(<1..317)
                     /locus_tag="IWI39_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9208821.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLE"
     gene            complement(504..1154)
                     /locus_tag="IWI39_001719"
     CDS             complement(504..1154)
                     /locus_tag="IWI39_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208822.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1697..2632)
                     /gene="whiA"
                     /locus_tag="IWI39_001720"
     CDS             complement(1697..2632)
                     /gene="whiA"
                     /locus_tag="IWI39_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9208823.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2666..3664)
                     /locus_tag="IWI39_001721"
     CDS             complement(2666..3664)
                     /locus_tag="IWI39_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9208824.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3661..4545)
                     /gene="rapZ"
                     /locus_tag="IWI39_001722"
     CDS             complement(3661..4545)
                     /gene="rapZ"
                     /locus_tag="IWI39_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9208825.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4680..5411)
                     /locus_tag="IWI39_001723"
     CDS             complement(4680..5411)
                     /locus_tag="IWI39_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9208826.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5415..6680)
                     /locus_tag="IWI39_001724"
     CDS             complement(5415..6680)
                     /locus_tag="IWI39_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9208827.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6943..9759)
                     /gene="uvrA"
                     /locus_tag="IWI39_001725"
     CDS             complement(6943..9759)
                     /gene="uvrA"
                     /locus_tag="IWI39_001725"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9208828.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9769..11763)
                     /gene="uvrB"
                     /locus_tag="IWI39_001726"
     CDS             complement(9769..11763)
                     /gene="uvrB"
                     /locus_tag="IWI39_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9208829.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12022..13524
                     /locus_tag="IWI39_001727"
     CDS             12022..13524
                     /locus_tag="IWI39_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9208830.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13526..14263
                     /locus_tag="IWI39_001728"
     CDS             13526..14263
                     /locus_tag="IWI39_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9208831.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14308..14481)
                     /locus_tag="IWI39_001729"
     CDS             complement(14308..14481)
                     /locus_tag="IWI39_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9208832.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14457..15122
                     /locus_tag="IWI39_001730"
     CDS             14457..15122
                     /locus_tag="IWI39_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9208833.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT         4425 a         3251 c         2574 g         4884 t
ORIGIN      
        1 ctctctagca taggaccgac aattgaagaa gtcactactg caaatgctgg acctatccaa
       61 gcaacattag aatgttcttt ctctcttttc ctttgtaaat ctttgttgag tattaatcta
      121 tttatttctt ctgcaggcaa gttaattttc ataaccttat gggggattat ccaattaaaa
      181 atcggaaaaa ggtagcctcc ccaaaaagta tagtgatctg tgtcaatcag ataataacct
      241 gattttgatt ccagaataac atatctcaaa gccgaacgaa aatttttcat gaatatccat
      301 aatctcactg tttccacacc taattcctcc aattccaatt aaatggattt atagcatcac
      361 caattatgtt aactgcctga ccaacctcat tgataaaatt ggcaccacta tcccatgctt
      421 caacaatgct atatatctat caaacaaaaa atggaaaact aaatactcgc gcacataaat
      481 tttacattct gttttttata agactaataa taatcattga aaaaatttct tatcggtggc
      541 gcatattccc aattgattaa tgtgatccca tattctgcaa gtattttacg agcttcttct
      601 aagtcatagt gagaaaaatg cataaaacct gcaatacgat cacggactgt gagctgtggc
      661 ttgcttccac catcattcat aaaatatcca gcagagccac tacttaaagt gattgttaca
      721 tcttcacttt ctattattaa gaattcataa tgaaatgctt tcaactctcc actattattt
      781 aaaagtttta tactccaact tcgtctaggt tgagttgtca cattccctct aaaacttcct
      841 ataaattctg caatcaccgt atcttcaatc aaaattcttt cctcatctat ttcatattca
      901 agactaaacg gaaattctcc atatgtaatc tctggcaatg gtggtgctgt agttctccaa
      961 ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct
     1021 gctcgaagta aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa
     1081 ataaaaagta ctgcaatcat aatgatagta aaagttgtaa aaaagcacat tttttgccct
     1141 ccctttataa acatcgattc tcaaattcta aaccaaatct tgatagttca agttcaggta
     1201 taacgtatac caaccataca tcccaattac tatttatgaa cttatgatta tcactgaata
     1261 aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag
     1321 ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag
     1381 taagaaaaac agttctcaag gttgatcgag cagttccttc cagtttttaa ttaatctcca
     1441 gttatagata aatacttgat cctatctata tcaggacttc tatattaagc aaaagtttat
     1501 gtataaaact aaaatcttca ttcagttgtc tccccgcttt ttatacgatt gtattaaaaa
     1561 acaatagaag cctttcaaca ttatgttact ccagtaaagt tggaattata gtttgacgga
     1621 agaaaagcaa gtatagcttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat
     1681 cagttttttc tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt
     1741 tgcgaattcg gtgattaatt cccgattttg atattgcacc tgagggaatc atttccccta
     1801 attctttcaa gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt
     1861 caggaagggc atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag
     1921 aagccgcatc aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg
     1981 aatttctcat atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac
     2041 caatcaatgt taagaaatca gcaatttttt ctgctccttt taaatacgaa atatagccat
     2101 tgcgccgttc aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt
     2161 ggttatgctg ttcataaata gaaaatattt ccaaatgata acgactcgtt tccggattat
     2221 tgactgaacc tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc
     2281 ccataatctc gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca
     2341 tcaacacatc ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt
     2401 tcattttttt acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat
     2461 agatacgtct ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca
     2521 caatacctaa agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc
     2581 gatgaacttc caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct
     2641 cctcctttct ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt
     2701 cttctactac tttgtctcca tcgtggaaga caccaccatc tttcaattct aaaaagtcgg
     2761 tggaaatcac tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta
     2821 gatactcatc atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca
     2881 atactgtatc gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat
     2941 ctgtgaaatg ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag
     3001 cagttgtctt gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg
     3061 tgaataaact tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt
     3121 ttcgagccgc tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg
     3181 ttttacggtt gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaaaacag
     3241 catgcaaggt caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt
     3301 ttgctaataa ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga
     3361 tcagattacc gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga
     3421 aaatatcttc atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac
     3481 caggaggagc catagcgatc gattctctga ttgcaccgct gcttccacca tcatctgcaa
     3541 cagtcactac tgctgtgata tctgctcctt gatttcgtaa acttttcagg ataactggca
     3601 gaccagtgcc accgccgacc actacgatct ttggttttct tatccggtag gttttcattt
     3661 tcacgaccgg ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt
     3721 atagtttttc cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc
     3781 gcctgtacag ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa
     3841 tacagtttcc agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat
     3901 cacataatca tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg
     3961 agggttgggc aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt
     4021 aaaaccaaat gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc
     4081 attgattttt tcacgtaatt gtcgtggtgt caatgttgtc gtatcaatga tcacggaagc
     4141 ttgggctttc aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc
     4201 gtccatcgcc attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc
     4261 tgaagcatct aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag
     4321 catgttttgg atttcttcaa agaaggaacg agaacgcaaa tccactccta aagccatttt
     4381 tgtgacttta cctgattctt tgatcaattc ccagaatttt ggaatcaatc gaggcggcat
     4441 attatcgata caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc
     4501 accactcata cctgttatga tgactaattg caaattatct gtcatattta cgaacccctc
     4561 tcacattgtg cataataaaa ttatatcatc ccgggcgtat cattaccagt tttctttttc
     4621 ttttacaaaa agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt
     4681 tacttttccg atgctgtatc caatgcctcg cttctttcag ctaatgcacg ttcgatcgtt
     4741 cgatccgcta aaatagattg agcatcgatc actgggtaat gattatcttc agcaaattca
     4801 tgcatcaaag ataattctgt gcaacctagg atgacttttt cacagtttag gcgctctacc
     4861 gcttcctcta agatttcgta atagagctct tgattcaaat aatccgattc cttgatctca
     4921 tgatagatca ggtaattgat tttttcttga agcgcagtat caggaatcac tgtttcgaaa
     4981 ccaagatttt tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga
     5041 atcgctactc tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata
     5101 tgcaagatag gaatatccgt tgccgcttgt aactcttcaa aaaagtagtg tgcggtgtta
     5161 caagtcaaca caatgaagtt tggtcgcaat aaattttgtt tctcaatatc atccagcaga
     5221 aacggcattg gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt
     5281 gcatgattga ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg
     5341 ttgatcaagc gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa
     5401 aaattctcca tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag
     5461 ttatggaaca tatatttcat caacaaccag cgcttgattg atcggtcttt ttcataaaat
     5521 acagtagtac cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt
     5581 tcccgagcat attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct
     5641 gattgattcg ttccataggt cgtttcaaca aagggtttgt tgaacactcg atcttctgtg
     5701 acaaaacgag ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc
     5761 aaattgattt caaataattt atattctcca tctctaggat catacttcat atcgaaattg
     5821 gcaaagcctg tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg
     5881 ttataatctg gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaat
     5941 agtggatgac caaggcacat catccgaact tgatgatctt catctacata cgcatttagc
     6001 acacgcatat tactatcatc acccgggata aaatcttgta caatcatttc acttttataa
     6061 ccggcttcgt atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa
     6121 gctttttttc ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa
     6181 gcaactggga attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct
     6241 ctgacgatca atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt
     6301 actttattga tcagcctttc aaataaagaa taatcaatat atggacagat aaaatattct
     6361 gacagctctt gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa
     6421 agatattttt tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata
     6481 aatacagggt ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg
     6541 gtaggggcta actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa
     6601 gaacgtgcca taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca
     6661 ttttcaatac tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca
     6721 actacacaag gaatatcata ccataaagaa aaaagcaatc aaaggaaagt cctccgattg
     6781 ctcaattctt ttttgaacgg attgaactta taggaagttt ttatctatac caatcagtgt
     6841 agaaagaact accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc
     6901 atttttttat gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt
     6961 caaatagtga cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc
     7021 aaggatcgtt cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt
     7081 tttgattaca tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct
     7141 ttgtaataca tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc
     7201 tagtatatag aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg
     7261 ttgtgcctca ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc
     7321 aacatcaaca atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac
     7381 agcatcctct actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc
     7441 taatgtttca gaattatatc gtttaccatg acatacttca caaggaacat agacatcagg
     7501 caaaaagtgc atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc
     7561 ttttacgttg aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg
     7621 agcaaataac ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg
     7681 cgtccggccg attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga
     7741 aattgtcttg aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt
     7801 ctttaagata ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac
     7861 aaattctcct aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt
     7921 gatcttgatt gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt
     7981 ccctgaaaga tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc
     8041 tgcagccacg atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcaaataatc
     8101 tgctgcccac atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg
     8161 cattttcttt aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg
     8221 ttcatctaaa atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat
     8281 tcgctgcgct tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag
     8341 tcccacattt tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat
     8401 tactttctct tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga
     8461 caactcattt gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt
     8521 caatcgataa cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc
     8581 acgcgtgaaa tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc
     8641 gaaagtcgtt tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag
     8701 atcatctgtc ccattcaaga tgatctcctg atgctcttcc ggtaattctt cgaatggtgt
     8761 atctaaatcg ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga
     8821 gctgattgga ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat
     8881 cactaaatct tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc
     8941 aaaaggcgca ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata
     9001 aggacaggca tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc
     9061 atagccgtct gctaatcgca gggccgcctc taaagaatca aataggcgag aacgaatccc
     9121 ttctttcaca atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag
     9181 ctcaggcact tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc
     9241 tttttggatt cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc
     9301 caacacttgg atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac
     9361 tgattgactg gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa
     9421 caacaagcgt aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt
     9481 acttgttgtt ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc
     9541 cggcttatcc atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct
     9601 ccgctgccct tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa
     9661 acctgtaacg accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg
     9721 ggcacgtgca ccatgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc
     9781 ttttaattct aaaatcatat ctcgcacatt cgcggctgtt tcaaaatcca aagcttttgc
     9841 cgcatctttc atttcttttt ctaatttcat caataacgtg tctttttctt ccttcgtcat
     9901 ttcttcgtac gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga
     9961 aatcaaatct cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta
    10021 cttctgctga atcgtacgac gtcttgacgt ttcatccatc gctagacgca tagaatctgt
    10081 tactttgtct gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt
    10141 ttggactaag gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac
    10201 aagggaaact tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc
    10261 aaattcacct aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact
    10321 atgcaaatat ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat
    10381 ttttttcgtc aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc
    10441 gcctactaga tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag
    10501 ccctgtcgga cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc
    10561 tggcgtcgca gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag
    10621 tggccgattg tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct
    10681 cgctcggtca ccattataca ttcctctgat ttgaggcatg gttacgtgcg attcgtccgc
    10741 cacgatcaaa aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt
    10801 acgtccgtcc atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat
    10861 catttcaata tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc
    10921 atttcgcaag acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc
    10981 catatgttca tcattcgtca caaagtgagt cgccgggaag attgctacgt gttccgtttc
    11041 tcctaaaact tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa
    11101 aaactccaca cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc
    11161 gcgcactcga aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac
    11221 taagtcacga atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg
    11281 tttttggtac tcaaaaggcg agcctaatcc aaaaatacat gaaacggatg caatgacgat
    11341 cacatcattt ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc
    11401 atttacactt gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg
    11461 ataataatca taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa
    11521 ttccccgtac agctgaccag ccaatgtttt gttatgggca ataatcagtg ttggtttatt
    11581 cactttctca ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa
    11641 gatttgtgct tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg
    11701 atcaccagcc ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat
    11761 cattcttcgt cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg
    11821 aaagcaaagg cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt
    11881 ttatcttatt atacatgtta tgttttataa cataaaatgt tagaaactcg catttttttc
    11941 attttttcga gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat
    12001 tggaaattta ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta
    12061 atgacaggac ttgtgacact tgcgtttggt catgttgcac aagctgacaa cactacaacg
    12121 gcagagtcaa gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta
    12181 attgccagtg actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa
    12241 ggcatcgatg taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg
    12301 gactaccgtg gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg
    12361 attgccggaa tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac
    12421 tttgatagcg ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa
    12481 gatttgaaag gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc
    12541 gaccatgaat ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac
    12601 gacgggttaa aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat
    12661 gcgatcaaac aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat
    12721 gcatttgccg ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg
    12781 aaagaattaa aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt
    12841 tcagaaactt ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa
    12901 aacaactatc aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc
    12961 tttgctttgg ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc
    13021 gctcttcgca caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt
    13081 cttgcgttct ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc
    13141 cttgctggtg ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga
    13201 ggcgggatca acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct
    13261 tataatcgga caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca
    13321 ttcgtcaatc agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta
    13381 gtcgaattgc tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt
    13441 tacttgatca ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa
    13501 gttttagaaa agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa
    13561 aaaatacggc gataacacag tattaaacga catcaatgta tccatcaacg aaggagatgt
    13621 cgtctgtatc attggccctt caggttcagg aaaaagtaca ttcttgcgtt gcttgaatcg
    13681 tttagaagaa ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag
    13741 cacagatatc aacttggtga gacaacatat cggaatggta ttccaacact ttaacctatt
    13801 ccctcatctt tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc
    13861 tagagaagaa gcagagaaaa aagcaatcaa attactgcag acagttggtc tagaagataa
    13921 aaaagatatg tatcctgaaa tgctttctgg tggacaaaaa cagcgtgtgg ctattgctcg
    13981 tgctctagcg atgaaccctg acatcatgct ttttgatgag cctacttctg cactcgatcc
    14041 tgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat
    14101 ggttatcgtt acgcacgaaa tggggttcgc taaagaagtg gccaatcgcg tcatgttcat
    14161 tgacgacgga aactttttag aagatggaac gcctcaacaa atctttgaaa atccgcaaaa
    14221 tgaacggaca aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgca
    14281 cggtcgccat gaagggaccg tgtattttta tcttcttatt ttctcaccta atcgtatcag
    14341 ccgcatcgct tgttttcctg cacaaacaag caatcgctct acctgttcca tcgcataaga
    14401 aagtaccatc ggttcattga tgatatcaag tacatagtcg ataccaccct cacttaattg
    14461 agcttgattt ttcttctcca tattatgtag ttgtgggaca actttctgac aatcagcaat
    14521 tgtgtcaaga acggatcaat cctttattgg agttgctcga aagggtcttc tcttattacg
    14581 gacaggcttt gctcactgta catagacaac aaatcattat cctagtcaat cggatttcca
    14641 gagcctcatt gctatcattg cttgataaaa tccagacaaa gtttaataaa atgtatcagt
    14701 tacagctaaa ctttggaatt gggtcgcttt gttatacaga gcaagagaca ccgcaatctt
    14761 tccttcatgc caaacaagtt tgcgaatgga tagctttcca tcaatctgtg aacgagatcc
    14821 gcttttttga agatttagac ctcgggatcg ttttaccagc aatcccgtca gatcaacgaa
    14881 cattatatgt aaaacgtatt ttgaaaagcc tgacagaaga agaagtacat ttgttcaaga
    14941 agactttggc atgcttcagt aaaaataacg gaagcatcaa aaattgctca gaagaattat
    15001 ttattcataa aaacaccttg caatatcgat tgaacaagtt ccattctctc actggttata
    15061 cccctagaaa ctatgacgat tatcatattc taaagctggc ttttttgttg gtacaaacat
    15121 gacaactaca aaaa
//
LOCUS       DACDOA010000057        14904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDOA010000057 DACDOA010000000
VERSION     DACDOA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.057"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..608)
                     /locus_tag="IWI39_001731"
     CDS             complement(27..608)
                     /locus_tag="IWI39_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9208834.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(614..1873)
                     /locus_tag="IWI39_001732"
     CDS             complement(614..1873)
                     /locus_tag="IWI39_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9208835.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1875..2921)
                     /locus_tag="IWI39_001733"
     CDS             complement(1875..2921)
                     /locus_tag="IWI39_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208836.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3156..3431)
                     /locus_tag="IWI39_001734"
     CDS             complement(3156..3431)
                     /locus_tag="IWI39_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9208837.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3674..4984)
                     /locus_tag="IWI39_001735"
     CDS             complement(3674..4984)
                     /locus_tag="IWI39_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9208838.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5166..6395)
                     /gene="rpsA"
                     /locus_tag="IWI39_001736"
     CDS             complement(5166..6395)
                     /gene="rpsA"
                     /locus_tag="IWI39_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9208839.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6530..7210)
                     /locus_tag="IWI39_001737"
     CDS             complement(6530..7210)
                     /locus_tag="IWI39_001737"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9208840.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7259..7894)
                     /locus_tag="IWI39_001738"
     CDS             complement(7259..7894)
                     /locus_tag="IWI39_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9208841.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7960..9363)
                     /locus_tag="IWI39_001739"
     CDS             complement(7960..9363)
                     /locus_tag="IWI39_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9208842.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9350..10381)
                     /locus_tag="IWI39_001740"
     CDS             complement(9350..10381)
                     /locus_tag="IWI39_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208843.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10412..10633
                     /locus_tag="IWI39_001741"
     CDS             10412..10633
                     /locus_tag="IWI39_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9208844.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10798..11535)
                     /locus_tag="IWI39_001742"
     CDS             complement(10798..11535)
                     /locus_tag="IWI39_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9208845.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11688..13028)
                     /locus_tag="IWI39_001743"
     CDS             complement(11688..13028)
                     /locus_tag="IWI39_001743"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9208846.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13265..14788)
                     /locus_tag="IWI39_001744"
     CDS             complement(13265..14788)
                     /locus_tag="IWI39_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9208847.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         4233 a         3196 c         2487 g         4988 t
ORIGIN      
        1 cttttttagt actatactat ttttaactaa ttgttatgtt cacttacccg gttacttttc
       61 ttttgtgtct gcaaaaggat ctttcggtta acttcatcag ataatttcaa aaaagcatcc
      121 ctatctccat cttctagtgc ttgatcgatt tgagcataaa gcatttttag ttttgcctct
      181 tcttcttttt cttgaaaata tcgttcaatt aattcagtat cttcttcact gactgaagaa
      241 tcccaagaag aaaatggatt gtcttctaag atagatagat attctcttgt ctgccaagca
      301 ttttcgaata tacattccac atataaaggc tttttccaat tcaaacggat ttcatgaaaa
      361 atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca atttcatggg ttccccttca
      421 acagatagat cactgatctg cagtcctcga tccgcaaaat tcgccccttc aacaaagtgg
      481 acattgctta aaatcgcttc atgattggca agataattca agatccaaaa aacttctctt
      541 cggctaaaag atacatgatt gaccatccac gttaaaaaat tcttcttttc ccgtacatca
      601 attagcattt ttctcacctc tctgttaggt catctaataa tgacagtgcc tccatatctc
      661 ctggttcaag tgttaaataa taagtcaata attcttgcgc tcttttcagc tgcccttctt
      721 ctcttaagaa aaaggcatac tctttcaaga aatctggttc atgtgatagt tcatgataag
      781 cctgttcata gtgtacagct gcaacggtaa agtcttctaa ttcattataa gcatgggcta
      841 ggttccattc tgcataagga ttagcagttt cttccatctg attgatcact tgtatcacat
      901 cttcatatct ttcatcatcc aagtaaagat tacttaaagt aagtaatgtt tcatcttgtt
      961 tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc tcgctgtcta tcatgcagac
     1021 ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg gtaaggattt tctttgattc
     1081 cttcttctaa cactgtttgt gcttcttcaa tcatttcttc ttcttgaaga gcttgtccta
     1141 gatatagata taacgattga taatgcggat taacttctcg aagttcttcc agatagttga
     1201 ttgcctgctg attttctttc aattgaagat tcgtaaaagc tagctggaac agtcgatcat
     1261 ctgtcctttc ttctttcagt gcttcttcta aaggtacgat agctgcttcg aattctcctt
     1321 gcatacttaa ggattgtcct aaacgttcct gcatcgagat tcctgaaatt tcatttatcc
     1381 cgcttgccaa caatgtttca taaaccactg ctgcctcttt gaaacgatca actgaaaaat
     1441 acaattcagc tagcgcaaac tggatcaatg gttcatcagg aagcaaccca gctgcttctt
     1501 tcaattttgc ttcactcact tcagggattc cgataacttg gtacaaatca gctatcgcca
     1561 ataagctttg cggatagtaa tcactttgct tatccacttt ttccaggtat tcaaaagcta
     1621 agtcgatctc attatcttcg attgcaattt ctgctaacgg gatgttcagt ccgtcatttt
     1681 ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc ttcgatcaaa aagccaatag
     1741 ccagcaattc ttcgcctagt tcagctaata tatctgctgg gtcttctttt aatgcttttt
     1801 ctaaaaagat ttgtgcttgt gacaagtctt catctgataa agcttgcaac attttttcgc
     1861 tatttgtatt catttcaatt ccctcttatc tcactgacga tttgttgaaa taccgtcatt
     1921 gttctcaata attctttttc gtttggatgt tggtatccgt ctattatacc tattttttct
     1981 aaaacaagta tttttttgct tttttctgcc aaaaaaagaa ctttttccaa atactccatc
     2041 tttgaaatct gttccagcca gtggattgga tagccaagca agtccagcca cacaaaaaag
     2101 ttttttagtt ggaaatcaaa aggatatgct tggagattcc acacgaaatg aaacatcatt
     2161 cctaaaaatt ttttcatact agcagaaagt agatggccat tttcggtcag ataaaaagct
     2221 tgttcaaaca gtttaccata atttgacagg ttttctgcct ctaattgata aaaatagatc
     2281 atttcttcga tcatcccggc aaacggccga gtcgttaatt gctttttatt tggatagttt
     2341 ttatacagat tttgcaaaaa acgatgatca caaatcattc cgcaacagat aaaagtcaaa
     2401 aagtcaatct gctttccgtc cacctgccgc tcgctgattg tttgatcata aacgatgcgt
     2461 tctggcagat tgatggtttg cagcactaca cgatttggta gctgcaaaat cgtccgctgc
     2521 ggtgttaatg ctcggcttag agaacggatc gatacaggga tcatccatag tgatgaatca
     2581 agggttgtat gtttttgaaa aaaacctgcc agatccatga tcccttcatt tccaaatccg
     2641 atgataaaca tggattgatt ttcgggatag cgtttgacga aatcaagcag gttctttaat
     2701 tctgttaaat gattgcattg agtgttggca caaatatacc agtcattgtt tgcttgattt
     2761 gcaaataacg ggttgatctt atctgcaaac aaatcataat agcgctgatt cgttaaaaat
     2821 aagattggct gatcaatgga aagttctctt agctgactga ctagcgtttc tccgtaaaac
     2881 actttcgtca acgcatcttc tacttgataa tcaagttcca tacgcatccc tcctcttctt
     2941 atttttattt taccattagt aagcaaattc tagaaaagat aagagcaaag ctcacctcat
     3001 aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt ttctatgtat gacatacgga
     3061 ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag aaaagcactt
     3121 gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc ttttaatgct
     3181 ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc accagtttgt
     3241 gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa accgattaat
     3301 tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc atctactgct
     3361 gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat taattctgct
     3421 ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat atcatttagt
     3481 taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt caatattctg
     3541 ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt cacatgcttt
     3601 ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa aaaacgtcga
     3661 tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc ccttcaaaag
     3721 taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc atcaattcct
     3781 cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta gcataaaaaa
     3841 tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca tccataatga
     3901 tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact tgttcgatca
     3961 tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg attggagcgt
     4021 aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt gtattggttt
     4081 ctttctttac aagatcccat ttattcacga cgataataat tccacgacca gcttcatggg
     4141 cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc agtaccatca
     4201 aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat ttttcagtat
     4261 tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag aatttctgac
     4321 cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca atatccgaaa
     4381 caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca acatttgggc
     4441 gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct tcagagaaat
     4501 acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct gaaacaggat
     4561 aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt tctggattat
     4621 ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt ttcgcaacca
     4681 tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt acatctgctt
     4741 cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca tcgccaagat
     4801 caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct tttgcataaa
     4861 tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca ccagcgatgc
     4921 ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt gttggatttg
     4981 ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat gcattgccaa
     5041 ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga aatactaatc
     5101 gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca cagatacaaa
     5161 atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt caacgcatca
     5221 cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata agactcaaca
     5281 ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact caatgcaata
     5341 cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc ttcatgaagc
     5401 acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac tagcccttct
     5461 actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac tgttccgtct
     5521 aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct
     5581 ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt gacatttaca
     5641 tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga aatctctgag
     5701 acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt
     5761 gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc ttttttcttt
     5821 tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt ttctgatggt
     5881 tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca
     5941 aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc aacgactaaa
     6001 ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg gaaatctctt
     6061 tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag atagctgccg
     6121 ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc accgactttt
     6181 actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg aacgactcct
     6241 tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac taaaacttcg
     6301 cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc ttccattgac
     6361 tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct aaccaacatt
     6421 ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa taaagcgatt
     6481 tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt taaaaaagaa
     6541 gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga atcgatttgc
     6601 cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg tcccttgttg
     6661 aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc
     6721 ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt gctactaaaa
     6781 agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg tccatgacaa
     6841 cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg acttttccat
     6901 gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct aaagtcacat
     6961 cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag ataggatact
     7021 ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc gtaatagcta
     7081 aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc aattcttttg
     7141 ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt gcaatactga
     7201 tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc ggctattatt
     7261 atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct aagccattca
     7321 attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt ggaccttcgc
     7381 ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt gtttctgctg
     7441 cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc ggtgttgtct
     7501 ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta gattcttccg
     7561 cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt gtacttgtag
     7621 aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca ttgttgcttg
     7681 atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag accagtaaaa
     7741 tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga gaacttcttg
     7801 atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt gtttcgttat
     7861 tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa ctctatcgtg
     7921 tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca tgtttttttt
     7981 aaataaattt agtaaaatcg ttccccaatg aagttgttca gtcttagaaa aaagcttcct
     8041 ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg gcttttcgga
     8101 gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt cattaatata
     8161 gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga gccgttccac
     8221 ccaattttct ggtttttcag actgctggat cagctccatc catttttttt gcagctcatc
     8281 aaagggagct tgctctgtgg catattccaa ataaagctca aaactttgtc gctgttctct
     8341 agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa aaagaattgc
     8401 tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt tttctagact
     8461 gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc ccataccaaa
     8521 tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt gaagttgtct
     8581 tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt ccctgaataa
     8641 atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg ctccttgtgc
     8701 tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa caaaaagcga
     8761 gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa atagtacatg
     8821 acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt
     8881 tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat caactcccca
     8941 ttgagacacg caatgagcct catctactac atataacgca atattttgtt ttttcagatg
     9001 ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt tatattgcga
     9061 tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga aagcagctac
     9121 tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag aaagcagcgg
     9181 tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc aaaggctttt
     9241 tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg cctgaataat
     9301 ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt catttattaa
     9361 cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat tctccataat
     9421 ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa tccttatttg
     9481 gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa tcatcaaaaa
     9541 acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga cggattgcca
     9601 tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc atgctttgat
     9661 tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct ggccgttttt
     9721 cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc gctgactcat
     9781 cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca gataattggt
     9841 tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc aaactctcat
     9901 acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat ttttttagtt
     9961 ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc gttgccagat
    10021 tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct gttctgccat
    10081 agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata tcagcccctg
    10141 caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc gcaatttttt
    10201 gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg ataaacaaaa
    10261 gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca aggagttggt
    10321 aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata aaaacaatca
    10381 tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac ttgtacctga
    10441 acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg attatgatga
    10501 agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg tgatgccgga
    10561 agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga ttttattaga
    10621 aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc taaacttgaa
    10681 aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg aaagattcat
    10741 ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc atttatatta
    10801 tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct ggtctgcgtt
    10861 agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac ctccatgatt
    10921 tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag cttcttgata
    10981 agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag gaacagctga
    11041 ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc cttttaaatg
    11101 tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta atgaatggcc
    11161 accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc
    11221 catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt tttttgctaa
    11281 atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat aaaaaataag
    11341 taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat cttctttgtt
    11401 ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat
    11461 atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa ttagtattcg
    11521 tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt tcttaatcaa
    11581 gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat caaataaaaa
    11641 aagacggaat atccatttga atatccgccc tttttgtatg tttattttta tttagcacat
    11701 aaacgataca cagcttctgc ataaatttcc attgctttat acatactact taattcccat
    11761 cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc gaatgccaca
    11821 cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc agtcatgtct
    11881 ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc tagtggcacg
    11941 tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc tgctgtttta
    12001 gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat atctacaccg
    12061 atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag ctcgccggat
    12121 tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg aactaatttt
    12181 cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc cagacgagaa
    12241 atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag aacgacgatg
    12301 ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt tacttcagaa
    12361 agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc tccagctttt
    12421 acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc tttttctgca
    12481 taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc ttctttttcg
    12541 ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat aaaacgaata
    12601 cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat tgccgcaata
    12661 gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt tatagttgga
    12721 tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc catatgacac
    12781 aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata gccttctgga
    12841 tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc caaagcttca
    12901 atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag tacggaagga
    12961 attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc tgtcacaaat
    13021 tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt cctccgacac
    13081 aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc agaatgttct
    13141 taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttat ccacgtgctt
    13201 atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct gtatttgtct
    13261 aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat aatatcgcaa
    13321 ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt gctacagcac
    13381 ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca gattgataag
    13441 cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc atgattccca
    13501 ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg atatagaaaa
    13561 taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt tcgccccaat
    13621 gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca cgagcaacag
    13681 ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca gagacaaatt
    13741 cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag aataacatag
    13801 taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga accgtaccta
    13861 gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa ttcgcattca
    13921 aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag aacaatacat
    13981 taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca atatcgaaat
    14041 gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct ttttcgatct
    14101 ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg aacatcaaga
    14161 atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct acgcctgttg
    14221 caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat acgattgcta
    14281 cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa gcatagaatg
    14341 gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac agcatcaata
    14401 ctggaataag cattttttct tttcctttga atttcttcac aactgaagca atcccggcat
    14461 ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa atgaagaaag
    14521 agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca cctagcatcc
    14581 cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt actcgttcat
    14641 atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct ggtataaacc
    14701 aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct gaaggcattt
    14761 gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg ttagagacaa
    14821 caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat aataacacgc
    14881 attttgtaaa acttttgttc aaaa
//
LOCUS       DACDOA010000058        14872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDOA010000058 DACDOA010000000
VERSION     DACDOA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.058"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(510..1076)
                     /locus_tag="IWI39_001745"
     CDS             complement(510..1076)
                     /locus_tag="IWI39_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9208848.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="IWI39_001746"
     CDS             complement(1076..2032)
                     /locus_tag="IWI39_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9208849.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="IWI39_001747"
     CDS             complement(2032..2688)
                     /locus_tag="IWI39_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9208850.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="IWI39_001748"
     CDS             3058..5076
                     /locus_tag="IWI39_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9208851.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5503..7917
                     /locus_tag="IWI39_001749"
     CDS             5503..7917
                     /locus_tag="IWI39_001749"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9208852.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="IWI39_001750"
     CDS             8265..9818
                     /locus_tag="IWI39_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9208853.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="IWI39_001751"
     CDS             complement(10199..11983)
                     /locus_tag="IWI39_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208854.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="IWI39_001752"
     CDS             complement(11998..13719)
                     /locus_tag="IWI39_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208855.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="IWI39_001753"
     CDS             13819..14580
                     /locus_tag="IWI39_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9208856.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4830 a         2738 c         2946 g         4358 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag at
//
LOCUS       DACDOA010000059        14740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDOA010000059 DACDOA010000000
VERSION     DACDOA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.059"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            76..1035
                     /locus_tag="IWI39_001754"
     CDS             76..1035
                     /locus_tag="IWI39_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9208857.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1541..2143
                     /locus_tag="IWI39_001755"
     CDS             1541..2143
                     /locus_tag="IWI39_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9208858.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2159..2836
                     /locus_tag="IWI39_001756"
                     /pseudo
     CDS             2159..2836
                     /locus_tag="IWI39_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3348..3644
                     /locus_tag="IWI39_001757"
     CDS             3348..3644
                     /locus_tag="IWI39_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP9208859.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3645..4061
                     /locus_tag="IWI39_001758"
     CDS             3645..4061
                     /locus_tag="IWI39_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9208860.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4081..5625
                     /locus_tag="IWI39_001759"
     CDS             4081..5625
                     /locus_tag="IWI39_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9208861.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5768..5992
                     /locus_tag="IWI39_001760"
     CDS             5768..5992
                     /locus_tag="IWI39_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208862.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6007..6876
                     /locus_tag="IWI39_001761"
     CDS             6007..6876
                     /locus_tag="IWI39_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9208863.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6857..7591
                     /locus_tag="IWI39_001762"
     CDS             6857..7591
                     /locus_tag="IWI39_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9208864.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7624..8532
                     /locus_tag="IWI39_001763"
     CDS             7624..8532
                     /locus_tag="IWI39_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9208865.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8529..9071
                     /gene="sat4"
                     /locus_tag="IWI39_001764"
     CDS             8529..9071
                     /gene="sat4"
                     /locus_tag="IWI39_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9208866.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9164..9958
                     /locus_tag="IWI39_001765"
                     /note="aph(3')-IIIa"
     CDS             9164..9958
                     /locus_tag="IWI39_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9208867.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(10066..10254)
                     /locus_tag="IWI39_001766"
                     /pseudo
     CDS             complement(10066..10254)
                     /locus_tag="IWI39_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10234..10806
                     /locus_tag="IWI39_001767"
     CDS             10234..10806
                     /locus_tag="IWI39_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9208868.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            11146..12000
                     /locus_tag="IWI39_001768"
     CDS             11146..12000
                     /locus_tag="IWI39_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9208869.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            12099..12308
                     /locus_tag="IWI39_001769"
     CDS             12099..12308
                     /locus_tag="IWI39_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9208870.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            12325..12597
                     /locus_tag="IWI39_001770"
     CDS             12325..12597
                     /locus_tag="IWI39_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9208871.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12599..13462
                     /locus_tag="IWI39_001771"
     CDS             12599..13462
                     /locus_tag="IWI39_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9208872.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13725..14462)
                     /gene="erm(B)"
                     /locus_tag="IWI39_001772"
     CDS             complement(13725..14462)
                     /gene="erm(B)"
                     /locus_tag="IWI39_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9208873.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14587..14670)
                     /locus_tag="IWI39_001773"
     CDS             complement(14587..14670)
                     /locus_tag="IWI39_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9208874.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5230 a         2316 c         3032 g         4162 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      361 cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      421 atatttgata ttgacacact accgatgaga ggtaaaagaa aacccaatgg ccatcaggaa
      481 aaacggggaa aacaacagta tcagcgctca atccatgatc gatctgataa ttatcctgat
      541 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      601 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      661 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      721 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      781 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      841 aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat ggattttaga
      901 aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa
      961 tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat
     1021 tctagcttaa tttgacaaat catactatga tacgtgtttg tttttctgtg ctgtttagtg
     1081 aatgattagc agaaatatag agagtaagat tttaattaat tattaggggg agaaggagag
     1141 agtagcccga aaacttttag ttggcttgga ctgaacgaag tgagggaaag gctactaaaa
     1201 cgtcgagggg cagtgagagc gaagcgaaca cttgatcttt taagttgcta tctttttaag
     1261 gtcaatagag tatacttatt tgtcctaata tggttttagc agtataattg acttggtgaa
     1321 taggtcattt aagttgggca taataggagg agtaaaatga aaaaatttat ttatcgagtt
     1381 ttagaaaatg acgaagtggt ggctattttt aatgagcaac aatatgcgca agattttatc
     1441 gcttacgaaa agacaatttc tgataagcaa tttgaaattg aaaaagtaga tatagctgat
     1501 tggttattgc aaccgagaga attttagagg ttggttgaaa atggctaaaa ttggttatgc
     1561 acgtgtcagt agcaaagaac agaacttaga tagacaactg aaagcgttag agggcgtttc
     1621 taaggtcttt tcagacaaat taagcggtca atcggtcgaa cgcccacaat tacaagctat
     1681 gcttaactat attcgtgaag gggatatagt tgttgttact gaattagatc gattaggacg
     1741 aaataataaa gaattaacag aattgatgaa tcaaattcaa attaaggggg caaccctgga
     1801 agtcttaaat ttaccctcaa tgaatggtat tgaagatgaa aatttaagac ggctgattaa
     1861 taatttagtg attgaattgt ataagtacca agcggaatct gaacgcaaac gaattaaaga
     1921 acgccaagcc caaggaattg aaattgctaa gaaaaaagga aaattcaaag ggcgacaacc
     1981 taaattcaaa gaaaatgatc cacgtttaca acacgctttc gatttatttt tgaatggttg
     2041 ttccgataaa gaagttgaag aacaaactgg aattaatcgc cgaacgttta gaaggtatcg
     2101 atcaagatac aacgtgacag tagatcaaag aaaaaaacaa tgaaaagagg gatagttaat
     2161 gagtacggtt attttagctg aaaaaccaag ccaggcatta gcctacgcaa gtgctttaaa
     2221 acaaagcacc aaaaaagacg gttattttga gatcaaagac ccactattta cagatgaaac
     2281 gtttatcacc tttggttttg ggcatttagt cgaattagca gaaccaggtc attataacga
     2341 aaagtggcag aactggaaac ttgaatcttt gccgattttt cctgatcgat acgattttga
     2401 agttgcaaaa gataagggaa agcagtttaa aattgttgct gaacttctca aaaaggcaaa
     2461 tacaattatt gtcgcaacag atagcgacag agaaggtgaa aatatcgcct ggtcgattat
     2521 ccataaagca aatgcctttt ctaaagataa aacgtataaa agattatgga tcaatagctt
     2581 agaaaaagat gtgatccgaa gcggttttca aaatttgcaa ccaggaatga attactatcc
     2641 cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca tgaacgcaag
     2701 ccctttatat accttgaatt tacaacaaaa aggcgtacaa ggtacatttt cattaggacg
     2761 tgtacaaaca ccgactttat atctcattta tcaacgccag gaagcaatag aaaactttaa
     2821 aaaagaacct tttttcctga ataattcata gttttactaa tagtctttcc taacattgat
     2881 ttaatagtct ttaaagtatt ctgcttcatc acccattagg tgatgaaaaa agaacccctt
     2941 tctgatttag cggacaaata ctttgaccta atctcagaag atataaaaga aaaaataata
     3001 aaatatccga tttatacttt gtataaagaa ctaaagtagt tagcaaatca tttacagcaa
     3061 atcactatgt tccacatacc attttacata cttaccttat ccacgacaca tgttgagata
     3121 gtatcgaaga taaagaaagt tgctccatgt tgtttttgcg agatggggaa atcaaagcca
     3181 cattgacaac catgatgaat aagcttgctt tcagtcataa gctaatccta gaaccacttt
     3241 tcaagtcagt tagccaaatc gatgaagaaa gcgaccgtga aagaatggac gctattgata
     3301 agctaatgga gcaactattg gaagaacgca atacccttat tgcactgatg tccaaaggtt
     3361 tcctagaacc tgctcttttt aatcaggaac gaaatgtttt agatagtgag attaaaaatc
     3421 ttactaccga aaaaacaaac ctggtaacga actctgcgag tggggttttg cgagcaaacg
     3481 agataaagga cctcattaat tatgtgtcag cagataattt taatggtgac tacacggaag
     3541 aactatttga agaatttgta gtgaacatca ttgtaaattc cagggatgag ctgacattca
     3601 atttgaaatg cggtctttcc ctgaaagaaa aggtggtgag ataaatggca tatattccat
     3661 atggatacaa aattcaagat ggagtggtta ctgtcgatga aaaggcagca ggtcaagtaa
     3721 aggtattctt tgagaaatac atatcaggac tatcccttac agtggctggc gaacaggcag
     3781 gtattgaaaa gacacattct gtgatggggc gcattttgaa aaacgtcaac taccttggaa
     3841 atgatacgta tccagcaatt attgataaag agatatttga taaagctgaa gaagttagag
     3901 ataaacgtgc aaaggattta ggacgagtgg tagagcttgc cgctttcacc tctccccctc
     3961 ccaaagaacg attcaaaatg aaaaaggcag ataataagat gccagtggat ccttttgaac
     4021 gagcagaata cttatacagt ctgatagaaa gcgaggaata aagtgacaga gaaaaatata
     4081 atggttattc ctgctcgtaa aagagtagga agtacagccg caaaagaaaa gataaagaaa
     4141 cttcgtgttg ctgcctattg ccgtgtttct acagaaacag aagaacaaaa ttctagctat
     4201 gaggttcagg tctcacacta tacagagttt ataaagaaaa ataatgaatg ggagtttgct
     4261 ggcatctttg cagatgatgg tatctctggt acgaacacta aaaagcgtga cgaatttaat
     4321 cgcatgattg cagagtgtat ggacggtaac atcgacatgg ttattacaaa atccatcagc
     4381 cgatttgcgc gtaacaccct agactgcctt caatacatta gacagctcaa ggataagaac
     4441 atatcagttt attttgagaa agagaacatc aacaccacgg atgccaaagg tgaggttttg
     4501 ctgactatta tggcatcttt ggcacagcag gaaagccaga gcctttcaca aaacgttaag
     4561 cttgggctac agtaccgata ccaacaagga aaggtgcagg tcaaccataa gcgatttatg
     4621 ggctacacga aagatgaaga tggaaattta actattgtcc ccgaagaagc tgagattatc
     4681 aaacgcatct accgagaata ccttgagggt cagagtctag tgggcattgg tcgagccctt
     4741 gaaaaggatg gtattttaac agcagcggga aaaccaagat ggcgaccaga atcagttaag
     4801 aagatacttc aaaacgaaaa atacatcgga gatgcccttc tgcaaaagac tgtcacagta
     4861 gattttctga ccaagaaacg agtgaaaaat gaagggcatg ttccccaata ttatgttgaa
     4921 aatagccatg aagcgattat tcctaaagag ttatttttgc aggcgcaaga agaaattcat
     4981 cggagaagca atatctacac aggagaaggc aagaacaaaa gaatttacag tagcaagtac
     5041 gctttaagtg ccatcacctt ctgtggagat tgtggcgata tttatagacg ggtctattgg
     5101 aatatccacg gaagaaaaga atttgtctgg cgatgcgtga ctagaatcga gcaaggtcct
     5161 gaagtttgta agaaccgaac cgtaaaagaa gatgaacttt atggtgcagt aatgactgcg
     5221 attaataagc tacttgcagg tgggaacaat atgataaaga cactggaaga gaatattcat
     5281 gctgtgattg gcgaaaccac agaataccaa atttcagaga ttaacaactc actagaggaa
     5341 aaacaaaaag aactaatcaa gctggcgaac aagggtcaag actatgacca cttagcagat
     5401 gagattgatg agctgagaga caagcgacag atccttttag tagaagatgc ctccctcagt
     5461 ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgcacc
     5521 ttagaatatg atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaaggc
     5581 cacttcgtca tagccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat
     5641 agcccatgac tctacagtag agttgtgggt tttcttttgc ttgctgatta taataatatt
     5701 ttaataacta ttgtgtttat caaccaaatg gtttataata ttcttagcgt aaacttggag
     5761 gtgctgtatg actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt
     5821 ttccgaactt gctagacgta ttggccagac tccacagaat ttcaataaaa aattacaacg
     5881 agaaacagta accttggatg agttgaaggc cattgctgat gtgctaggtg tcaagtttgt
     5941 gcaggcattt attttaccag atggagaaga aataaaaata tctaacgaat aaaggaggat
     6001 gtttatatgg taggtaatgt tattcaaata gtaacagaga aattatcatc tttgcctttt
     6061 atagaaggca tcgtattagg gggctcacgt gcaagaggca cccatacaga aaattctgat
     6121 atagatatcg gaatctatta caattcagac tcatttgacc tgacagcgat taatcaaatt
     6181 gctacagagt tggatgatga gaatagaaac aaccttgttg tacctcccgg agcgtggggt
     6241 gattgggtta atggcggtgg atggttagtt ataaacgggt gtcatgttga cttgatatta
     6301 cgtgatataa aacgggtgga acaaataatc aaagatacgg agcaaggaat tgttactgcc
     6361 aattatcaga ccgggcatcc ccatggttat ataagtgcta tgtatcgtgg agaattagcg
     6421 attagtaaaa tactatatgc taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa
     6481 atttacccta ctgctctaaa gaaaagcttg atgaactttt ttatatttga agctgaattc
     6541 tctttaatgt ttgtaaaagc aaatgcgggg gcagaggata aatattatat tgcaggccat
     6601 gtttttcgta ttatttcatg cttaaatcaa gtactatttg catgtaataa tgcttattgc
     6661 attaacgaaa agaaagctat aaaactgctt gaaacttttg aatataaacc tgaaaaatat
     6721 gccgaaagag ttaatcatat ttttgaagta ctcggtcttt ctctttttga atgctacgac
     6781 atgaccgaga aactttataa agaagtgaat gaaattgtat cggagataaa taacttttta
     6841 aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata
     6901 cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa
     6961 aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg
     7021 gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca
     7081 taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg
     7141 tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc
     7201 gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc
     7261 tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga
     7321 ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga
     7381 gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac
     7441 cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca
     7501 gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat
     7561 gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg
     7621 taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa
     7681 cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa
     7741 tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat atctaatgat
     7801 gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta
     7861 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
     7921 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
     7981 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
     8041 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
     8101 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
     8161 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
     8221 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
     8281 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
     8341 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
     8401 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
     8461 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
     8521 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
     8581 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
     8641 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
     8701 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatctttact
     8761 accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac cggtacgctt
     8821 atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta
     8881 tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc
     8941 aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa atcggctccg
     9001 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     9061 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     9121 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     9181 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     9241 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     9301 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     9361 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     9421 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     9481 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     9541 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     9601 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     9661 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     9721 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     9781 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     9841 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     9901 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     9961 cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct aatcttttct
    10021 gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa agttcgctag
    10081 atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga gtatggcttt
    10141 tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc ataggatata
    10201 ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag agtaacctct
    10261 attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc acagcgattt
    10321 aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc cggaatacca
    10381 attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact tccgccggta
    10441 atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt aaattgccat
    10501 ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa atctattttg
    10561 aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt gtacagcaca
    10621 tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac tgcaatctgc
    10681 aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga
    10741 atgatagagc ctatatcact ggggttttat gaacagaact ggtacttaat cggttttgca
    10801 ggctaagaaa tgaatatagg aattttcgtg tggatcggat taaaagcatt tgcatagaag
    10861 aagagctctg tcaaagccat gatggctctt tggaacaaat attaaaacaa atgctttcat
    10921 ataaaaaact atatgcccta caatgccgtt gtaaaaattg gggaaaaggg atatattgat
    10981 tttatatctt tctcaaaata aatataaaag ccctttaaag agggctttta tatattaatc
    11041 acaaatcact tatcacaaat cacaagtgat taatcacaaa tcacttgtga tttgtgattc
    11101 ttaatgatac aatattacta tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa
    11161 ctaaaaatac ttgaagaatt aagacgtatt ttaaacaata aaaatgaagc aattattatc
    11221 ttgaataatt actttaaagg tggtgttgga aagtccaaat tatcgactat gtttgcttac
    11281 ttgacagaca aattgaattt aaaagtttta atgatcgata aggacttaca ggcaacattg
    11341 acaaaagact tagcaaaaac ttttgaggta gaattgccac gtgtcaattt ttatgaaggc
    11401 ttgaaaaatg gaaacttggc ttcttctatt attcatttga ctgataattt agacttgatc
    11461 cctggcacgt ttgatttgat gttactgcca aaattaactc gctcatggac gtttgaaaat
    11521 gaaagtagat tgcttgctac tcttttagca cctttaaaaa gtgactatga tcttattatt
    11581 attgatactg taccaacgcc aagcgtttat acaaataatg caatcgtggc gagtgattac
    11641 gttatgatcc ctttacaagc agaagaagaa agtacaaaca acattcaaaa ctatatttcc
    11701 tatttgattg atttgcaaga acagtttaac cctggactag atatgattgg ttttgttcct
    11761 tatttagttg atacggacag cgcaacgata aaatcaaacc tggaagaact gtacaaagaa
    11821 cataaagagg ataatttggt tttccgaaat attatcaagc gaagtaataa agtaagtact
    11881 tggtctaaaa atggcattac agaacacaaa ggctatgaca aaaaagtttt gtctatgtat
    11941 gagaacgtat tttttgaaat gattgagcga atcattcaat tagaaaatga aaaagaatag
    12001 aatcacaaat cacaagtgat taatcacaaa tcacttgtga tttgtgatag gtgatgataa
    12061 aataaatagt aagaagaaat agaaagaagt gagcgatcgt gggaaattta ggcgcacaga
    12121 aagcaaaacg aaatgatacg ccaatcagcg caaaaaaaga tataatgggg gataagacgg
    12181 ttcgtgttcg tgccgacttg caccatatca taaaaatcga aacagcaaag aatggcggaa
    12241 acgtaaaaga agttatggat caagccttag aagaatatat acggaaatat ttacctgaca
    12301 aactttaaaa ggagtgaaaa tgaaatggca gttacgtatg aaaaaacatt tgaaattgaa
    12361 atcattaatg agttatcggc aagtgtttat aatcgggtat taaattatgt tttgaatcat
    12421 gaactagata ctaaaaatac tcgtttacta gaagtgaatc ttttaaatca attagaagtg
    12481 gcacaagaag ttgatttatt tcaacaacca tttgaagaat tacaagctat tcatgagtat
    12541 tggcggtcaa tgaatcaata ttcaaaacaa attttgaata aagagaaagt ggcttaacat
    12601 ggcgaatatt actgacttca ccgaaaagca atttgaagat cgtttagaaa agaatgttga
    12661 acgactaact aaaaatagac tagcggttga atcgccaacc gcttttttac ttggtgggca
    12721 accagggtca gggaaaacca gtttgcgatc agcaattttt gaagaaacac aagggaatgt
    12781 tattgtcatt gataatgata cctttaaaca acagcaccca aattttgacg aattagtgaa
    12841 actctatgaa aaagacgtag taaaacacgt taccccttat tcgaatcgca tgacagaagc
    12901 acttattagc cgtttaagcg atcaaggcta taatttagtg attgaaggaa caggacgaac
    12961 aacagacgtt cccattcaaa cagccacaat gcttcaatct aaaggttatg aaacaaaaat
    13021 atacgtcatg gcagtaccga aaattgaatc atacttagga acgattgaac gatatgaaac
    13081 catgtatgca gatgatccaa tgacagctag ggcaacacca aaacaagcgc atgatattgt
    13141 tgtcaaaaac ttaccgacca atttagaaac ccttcataaa acgggcttat ttagcgatat
    13201 aaggctttac aatagagaag gagtgaaact ctattcaagc ttagaaacgc cttccattag
    13261 tccgaaagag accttagaaa gagaattaaa tcgtaaagta tcagggaaag aaattcaacc
    13321 gactttggag agaatagagc aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt
    13381 taaagcaatt caacaaaaaa tggaaagctt gcagctacct acaccaccaa tacccaaaac
    13441 acctaaactt ccaggtattt aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt
    13501 aactactctt tttttataaa aacatttcct catgttgggg tcccgagcgc ctacgaggaa
    13561 tttgtatcga taagaaatag atttaaaaat ttcgctgttt ttttctaatt tcattttgag
    13621 gtgaattaat tggtagttat tagaggtgcc ctatacctaa taatttatct acattccctt
    13681 tagtaacgtg taactttcca aatttacaaa agcgactcat agaattattt cctcccgtta
    13741 aataatagat aactattaaa aatagacaat acttgctcat aagtaacggt acttaaattg
    13801 tttactttgg cgtgtttcat tgcttgatga aactgatttt tagtaaacag ttgacgatat
    13861 tctcgattga cccattttga aacaaagtac gtatatagct tccaatattt atctggaaca
    13921 tctgtggtat ggcgggtaag ttttattaag acactgttta cttttggttt aggatgaaag
    13981 cattccgctg gcagcttaag caattgctga atcgagactt gagtgtgcaa gagcaaccct
    14041 agtgttcggt gaatatccaa ggtacgcttg tagaatcctt cttcaacaat cagatagatg
    14101 tcagacgcat ggctttcaaa aaccactttt ttaataattt gtgtgcttaa atggtaagga
    14161 atattcccaa caattttata cctctgtttg ttagggaatt gaaactgtag aatatcttgg
    14221 tgaattaaag tgacacgagt gttcagtttt aatttttctg acgataagtt gaatagatga
    14281 ctgtctaatt caatagacgt tacctgttta cttattttag ccagtttcgt cgttaaatgc
    14341 cctttacctg ttccaatttc gtaaacggta tcggtttctt ttaaattcaa ttgttttatt
    14401 atttggttga gtactttttc actcgttaaa aagttttgag aatattttat atttttgttc
    14461 atgtaatcac tcctgaagtg attacatcta taaataaata cagaagttaa acgatttgtt
    14521 tgtaatttta gttatctgtt taaaaagtca taagattagt cactggtagg aattaatcta
    14581 acgtatttat ttatctgcgt aatcactgtt tttagtctgt ttcaaaacgg tagatgtttt
    14641 atctacatta cgcatttgga ataccaacat gacgaatccc tccttcttaa ttacaaattt
    14701 ttagcatcta atttaacttc aattcctatt atacaaaatt
//
LOCUS       DACDOA010000060        14450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDOA010000060 DACDOA010000000
VERSION     DACDOA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.060"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..381)
                     /locus_tag="IWI39_001774"
     CDS             complement(<1..381)
                     /locus_tag="IWI39_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9208875.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDAD"
     gene            complement(299..1828)
                     /locus_tag="IWI39_001775"
     CDS             complement(299..1828)
                     /locus_tag="IWI39_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9208876.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(1840..3255)
                     /locus_tag="IWI39_001776"
     CDS             complement(1840..3255)
                     /locus_tag="IWI39_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9208877.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(3248..4081)
                     /locus_tag="IWI39_001777"
     CDS             complement(3248..4081)
                     /locus_tag="IWI39_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9208878.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(4240..4470)
                     /locus_tag="IWI39_001778"
     CDS             complement(4240..4470)
                     /locus_tag="IWI39_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208879.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(5217..5684)
                     /locus_tag="IWI39_001779"
     CDS             complement(5217..5684)
                     /locus_tag="IWI39_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9208880.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(5759..6199)
                     /locus_tag="IWI39_001780"
     CDS             complement(5759..6199)
                     /locus_tag="IWI39_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9208881.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(6229..6522)
                     /locus_tag="IWI39_001781"
     CDS             complement(6229..6522)
                     /locus_tag="IWI39_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208882.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(6553..6747)
                     /locus_tag="IWI39_001782"
     CDS             complement(6553..6747)
                     /locus_tag="IWI39_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208883.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(6853..7305)
                     /locus_tag="IWI39_001783"
     CDS             complement(6853..7305)
                     /locus_tag="IWI39_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208884.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(7331..7792)
                     /locus_tag="IWI39_001784"
     CDS             complement(7331..7792)
                     /locus_tag="IWI39_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9208885.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(7993..8844)
                     /locus_tag="IWI39_001785"
     CDS             complement(7993..8844)
                     /locus_tag="IWI39_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9208886.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(8859..9659)
                     /locus_tag="IWI39_001786"
     CDS             complement(8859..9659)
                     /locus_tag="IWI39_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9208887.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(9702..10592)
                     /locus_tag="IWI39_001787"
     CDS             complement(9702..10592)
                     /locus_tag="IWI39_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9208888.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(10594..11535)
                     /locus_tag="IWI39_001788"
     CDS             complement(10594..11535)
                     /locus_tag="IWI39_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9208889.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(11768..12103)
                     /locus_tag="IWI39_001789"
     CDS             complement(11768..12103)
                     /locus_tag="IWI39_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208890.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(12429..12617)
                     /locus_tag="IWI39_001790"
     CDS             complement(12429..12617)
                     /locus_tag="IWI39_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208891.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            12695..12919
                     /locus_tag="IWI39_001791"
     CDS             12695..12919
                     /locus_tag="IWI39_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9208892.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(13211..13423)
                     /locus_tag="IWI39_001792"
     CDS             complement(13211..13423)
                     /locus_tag="IWI39_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208893.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(13426..13638)
                     /locus_tag="IWI39_001793"
     CDS             complement(13426..13638)
                     /locus_tag="IWI39_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208894.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            13913..14323
                     /locus_tag="IWI39_001794"
     CDS             13913..14323
                     /locus_tag="IWI39_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9208895.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            14336..>14450
                     /locus_tag="IWI39_001795"
     CDS             14336..>14450
                     /locus_tag="IWI39_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP9208896.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLI"
BASE COUNT         4023 a         3012 c         2236 g         5179 t
ORIGIN      
        1 atctgcatcc atcgcatatt ctgcaagaat tttaaataca tctgatcgta tttgtaataa
       61 gcgattgact tttgcatagt caaatggtgg aaaaaattca tcgataaact ctttgtattt
      121 tgcatcatac atttttaatg acttataatt tttttccaca tattcacgat attttttctg
      181 attacttgta ctgtaaaact ccatcatttc tggataagtg atattgtgta agtcagcttg
      241 tgataatagt tttgcttgta tctctttcag tgcttcagga aatacactag ttaatttttt
      301 aagtgtctga ttttcttgtt tgagccttgc ttgatcctcc agttctcgac gtttggtcca
      361 atacttcgtt tctatcgtca ctattgccac ctccgccaaa attgtattca ctatctggat
      421 aagcacttcc actttccaat tccatcattt cattttcata atcaacatct gttacaaatg
      481 gaatttggct ctgaatagtt cgtttggata cgtatggtgc aagtttaggt aaagcttcag
      541 ctaaataact taaatcagtt ggcaaagatc tagagaaagt aaaaataatt ttttcaggct
      601 caatttctac tttgtcatga aacttaagaa aagcagcaat tgtttctgcg cattctttca
      661 atccttctct aaaatattgc tctttagtat ttgttttcgc ttctaggcta atgatttgcc
      721 acttgcgagc ttctccagaa ctgtttgatt taaacacctc atcgttaaaa tcgattgctt
      781 tacaaatggt gtaaaactgt tttttcaata gatccatatg atattcattg aaatcttttg
      841 ctaaattttt tgtgacatat tcagcttttg cagtaggggc ttttaagttg ataatcccta
      901 gctgattcat catttcttta gcagtgcctt tgctcattgt tgtgccagta acaagcatgt
      961 acgcaagctt aaattgttcg atttcgtttt gttgatcaga caaagcacga tcaattgcat
     1021 ctccaatttc ctcagctact tcaaaatcac aataacgatt cgtattattt ttaaattctg
     1081 ccaaggtaat cgtccccaaa gggttatcgg tctcatcaat ttttttgaac gttccgcttg
     1141 taacaaaatt caaatcacca tagcaagcat atgtcagaat tttatttctt gtcataactt
     1201 tcatttcttg aaaaaatttt ttctggtaag agtcatactt ttctttgata tagattccgg
     1261 catttccata tctttctgct ctccaaggtt caatattact tgctcttaac ttccaaccat
     1321 catcttcttc cacaggctct agcaatcgga aagctactcc acaagctcct tgaaaagttg
     1381 cagtttcaga atcaagcatt gcgaatctca tggaatcaag attgcttgtt aatttgttaa
     1441 aagcttctgg aactgtcgga agttctatat cattttttaa gaatttatct tttaatcttt
     1501 gaatgagtgt tcttttttgt tccgatacat cgtaatccca tttgataggt ataccagtaa
     1561 aatgattgac tgcttggtct acgacaattg aatacatacc agcatgaagt ttattattaa
     1621 cttttacaat atcagtgtta ggctttggtc tattatctat ttcattcttt tcgctagtat
     1681 aagctagata ttttctttcg cgatcagcaa aaaatggttt catatcttct ataaaaccat
     1741 tcggatcaaa cattccatct tcaatttgtg ttgcatattt aacacgtagc ttcttatatc
     1801 tctctaaaga taatactgta ctaatcaaaa gctcacctcc taaaattgaa taaattgata
     1861 gtgacttggt ggctcataaa acgctaaagc cagtgcgtca gcaatatcgg gactaccaat
     1921 atttcgcttt ttcatatcat ctttactttc taaacgtata cgacttctac ttgtcatttt
     1981 gaatttgcga gtacttaatt ctttgattaa cgaactatca gaaggcaatt caataacagg
     2041 ctgttctccg ttaagatttg ctgtcatatt ttcttctaac atttccttga tgtttcccca
     2101 tagttgagta cctaaattat cgtaaaaatc gtcttctgat gttgaaccgt tattcactcc
     2161 aaacacctca aaaggataat gtttgtcttc tataagttct tctaggcggt cggttacacc
     2221 acctccgaca ccagtgtcat cgactttaat catcacttta tcaatactcg gatattgact
     2281 cattagattc ttggccatgt tgatgacata tcctgttgtt tccatggtgc tacgttttga
     2341 atacttctca tactccaatg ccctggtagc aattctagga aagagaatcg tagaatcatc
     2401 accatatcga gctacgtcga caccaatatg agcaaccttt gttttattga ctaaagaatc
     2461 actaatttgt ttttctgtag ccaattcaac tgtttcaaga ctgataaatg aatccaacgc
     2521 acctttcgga aattctccaa aaatacggac acgagcaaca tcactttctt ttccatattt
     2581 tttaagaatc atttctatat tgtctttgtt tgtacgttta ctatcatagc ttgatacttt
     2641 atgaactctg tatttatcac ggtctgaatt atgggaatca taaaagacac cttcaatatt
     2701 attggggttt ccacacatca acagcttatt atcaaaacct gatagcgtac caagaatagc
     2761 ttccataatt ggatcagaca caccagaagc ttcatctacc acaatcaaca tatggtcctc
     2821 gtgaaaacct tgcatacttt ctggtttagt cgctgttcta gccgtagcaa accaacgctc
     2881 tgaatcacca accatataaa ttttggtctt tgtccacttc agtaagtttt tgatcaagct
     2941 atcatttaac catttagcta cctctgccca aagtacatcg taaagttgct tcattgtcgg
     3001 agctgttgct attactttag cgtagggtcg acacgttaag aaccaaagta ttgctcctgc
     3061 ttctaatgct gtttttccaa ctccttgacc agaacgaaca gaaacctttg aaaattcagc
     3121 taaatcattt aaaacatttt cttgccattc atcaggattc aaatgcaaaa tatcttggca
     3181 aaaagctact ggtttatcat agtaataatc aatggcagaa ccaatatcag caaaaggaat
     3241 aaattcatta ttcattttct ttcaccgccc gtttgtttgc ggcatttaaa actgcctgtt
     3301 tccattcttc tatttcttca cctgaattat ttccgccaat ttcatttgtc tcagcttgga
     3361 tcttatcagt ctgagcttta attaaatcag ttctgtattc attcatataa agttcattca
     3421 tttgcttgat ggctttagct aactggttgc taatccgagt caatgaatct tcaattgaaa
     3481 gaatatcatc aattttgcga tgtttttttc tgctaatctg cacatcttgc ataacctcac
     3541 gcttgatttc tagcttttta ccattttttt caatcggagt tttaatcttt cttagctgct
     3601 gcaatcgttc gacttcttca tcatttagtc cagcttcagc ttgttggatt cttttcatca
     3661 ttcgaagttg tcttatttta agaagtcgta tttcttcgga caaagcaaaa gaaggatcat
     3721 cattcaaact agaatagatg tccttctcat tatcggttaa cgtgtcaaaa aatattgttt
     3781 catactcacc tgttttcaaa gcgtttttat tacccactgg cggagaagct cggctatttc
     3841 ctttgttacc tttagcattt tgattaccaa taggagcgcc accttgatta gtaacgttac
     3901 ttttgccatt ggtaacgtta ctaatcaatt cagcgctcca tttgtcttgc gatttccatt
     3961 ttctaacctg agaatctgaa acatttaatt cagatgcaat ttcctttaac tgcttttctc
     4021 cgttggattc taaccaaatt cttttggctt catcacgtct tggatcacgt tgtcttgcca
     4081 ttcaatacac accacctcac attctgttta ggttgagttt tgttttctaa ttttcaatat
     4141 cttttagcat taaatcagct tcaatcaata tctttaaatc ggaaactttg tctaacttga
     4201 tttgtcctga ttggagattt ttaagccatt tcttgcattc taatccctca aattcttgtt
     4261 aatattattt tgaatgtttg gttcatcata gaaaccatga ccgcaataaa cgagcttgca
     4321 cgcatcaatc tcacatggtt ctgcttctct ccccatttcg acaatagagt acttcccttt
     4381 gatttgtaca gaacgcacaa cacgcactga acaatcatca atggttcgag gatattcatt
     4441 agttagcgat acataccagt agtttctcat tatgtagcct cctttatgta aagaaaaaga
     4501 ccactcaaag agtgatctaa tatgtaatgc acaggcaggg acgtttccga tcctgtgctt
     4561 gagacatttg acgattcatt tgtaccgaat cccaaatctc aatctaacct aaccagttat
     4621 gtaatagcaa cctacacgat gcacaaaacg cgtacgaaat tgcgcacccc tatattttta
     4681 aaccgccgat gcctcggttg ccaaagtcac tggcaaggaa tcgaaccttg catggttgcc
     4741 gaagcattga cctagcacac atgcttagcg tctacccttt ccgccacagt gacactataa
     4801 aattattctt ggctgctact attttttatt ttgcccattt ttaaatccaa tcatatatac
     4861 attaagacaa agcgcaaaaa ttgaaattat taatggaatc atttctcttc acttaccttt
     4921 agttatcgtg tgaataatta aaaaaacaat agacagcaac ggatgataga taataagaac
     4981 aatttagaag gagttgaaat tcacatcctt attcttaata tttccgttgc tgcctatcga
     5041 agcttaatta aacgatgagg gagatttcct cccttacatt ttattttgtc gatcctgttt
     5101 cctaattttt cgacactatc ataatacaac gttgaatagg taagtgattg gtataaaaaa
     5161 ggtataaaat ggaaaccaaa ttggtaataa aagggtataa aaagtgtaaa aactggctac
     5221 ttgaaagcaa ccagttctaa cgatgaagca aattggatga taattctgtt tgattctact
     5281 ttaaccgatt cttcgctagt attattcctt tgagcagtta cataaatggg cagaccattg
     5341 atataacgat catagaatat cttcttgcgc ctttcagtca catcaggctt atgcggatgc
     5401 tgtatcgctg aatagcctcg aacaaacaat ttatgcagat actcaaattc ttcctgtgct
     5461 tcttctttat cgatcagcat tctttctgct tcaaatatat gatcagctgt agaaggtgga
     5521 accaaggaat aagatgctgt cacttttggt tctcgatgtt gccctactct acatctagct
     5581 gatagatatg ctgaaagaaa cacagcgaca ttatgttttg tgcgttccat atcaacatct
     5641 ttcgcgcttg gtgtctcata tttctttaca tcgaaaagta ccatcctctg attcccccaa
     5701 ttatgatata atacttatgt cggaaatatt attcatagtc ggaggaatcc gacttttttt
     5761 attttgtacg tgaaatgagt tctcctgatt tatacgcctc agcaaattca accaaagcta
     5821 ctgccttcat tctctcgata gttctatcgg aatatcctaa tttttctcct atttgataca
     5881 ttgacatctt attaggaaaa caataactat aataaagtac ctggaaatgg attctactta
     5941 gccgtgttaa tgcttcgata accgaatctc gagtacttat cgcttcaatg atttcttcgt
     6001 tttgactgtc aattgtgaat ttcaaatttt tggtaacagt catcacggaa aagtctattt
     6061 tcactggtcc gaccaaacgt tctaaatttc tgtatctttt tagtaatcgt ctagcatttt
     6121 tccttgtttg cttaatatct atattctcta aaatgttcac aattgcctct tctccttcca
     6181 gttgtggaat aacgctcatt gttgtaggat agttgtaaaa aagcaaaatt aagattttat
     6241 aacttccatg tccaccaatc tcaccactgc taaattctct ttgcttttcg ctaaccactt
     6301 atcacattcc atcgtgtttt caatgcgaat gattgctgag tgattataga cgtgttctac
     6361 atatccacga aatggataga tgaatccttc tgcttcgcag cgaaccatgt caccgacttt
     6421 gaattttggt ttcttacgtg ttttaggatt ctttgtcggc atatctagca ttaaaccgcc
     6481 gatgccgtgg ccgctggagt aaaatccgtc ttttagtttc attccgcttc ctcctcttta
     6541 gctgtcaatt acttactcgc aagtcactaa aatggctact tcatcgataa agtttccgtc
     6601 ttcgtcttcc attccttgct gaatgctaat gtcggtaatc tgcttaaaaa attcgttgtc
     6661 atagccttgt gcgtttatca ctacttcgac gtctccgaaa acctctttga gactattaat
     6721 tttatttact acttccgatg cgtccatgac tacacccctt tccagttaaa aatctctgtc
     6781 gttaatagac tactgttcaa agtattcatc ttcactcatc cttctgcctc ctctttagtt
     6841 gtcattagtg gactaaatac tacctgtttg aaaaccataa tgtgcagttt caacgccgtc
     6901 ataatcactt atttcccact cagccccatc gggtatatca acgactttaa gacttgcata
     6961 tggtccactg gcctcacttc ctagtttttc gacaacagca acgaggtcct tatcacctct
     7021 gtttgaatca cttgaaaaac tggaatcatc agcaattccg tacagtcggt caatttcgct
     7081 ataaattcct gtaactttgt cgattttcgg gtcttttttg aaatagtaga taaaatccat
     7141 cgaacctagc ttttggcctg tgtatctcgt gtattcatca tctgagtcat agctaaagtt
     7201 attgatgtat gggaatactt caatattttt tcttttaaaa atttccattt ttgcttcatg
     7261 gctcaaccca aagccaccaa aacatttatt taatacaatc ttcattactt ttcctccttt
     7321 gtttcctccg ttagctgaat tttttcataa acacaagcca atgtgttttg cttcgtttat
     7381 taccaaacaa tggctgttct ccaatagcct ctaacacatc agaaagtggt atctgttcct
     7441 cgttccactt aaaaattaat gttcctgaag gtcgtaaaac tcgcatacac tctgcaaaac
     7501 cttgcctgat gtcttctggc caaagttcgt ctaattttcc atattttttg gcaagccatg
     7561 agttttcgcc gacatgtctt aagtgtggcg ggtcaaatac aaccatatcg aacacatcat
     7621 cttcaaaagg catatcgcga aagtctgcga ccacatctgg atcaacattg atcacatgac
     7681 ctgttgataa ttcttcatac tgctgacgac aatccataaa tgttacgtca gaattgttct
     7741 tatcaaacca gaacaatcta ctaccgcaac atgcatctaa tatttttggc aattactcta
     7801 cctccaataa ctctggattc tcgtagacgt tacctaaaac agtgagaaat ctttctgtat
     7861 cccgataaat aattagctta cttatcttcc gaaccattcg actctcttgt tgagataatt
     7921 cgtccatgtc acgatcacct ataatttgca acattatctg acgacagatt cttttttctt
     7981 gtttagtcaa attcatgcga tcgctccttt gactggtttt atgcgcttgt ctgctgtttg
     8041 agaaaatacc atcgtaaatc catcagagtt gacaaacatt cttgatattg ttctcgtacc
     8101 gtaagctttt ttcagttctt caccaagcaa gttcgttgtg ataatcgttg ctaggttctg
     8161 tcgtgcatcc aagaacgaat ttagcgtgtt tatgccaaac gctcgactat ctgatgcatc
     8221 ttttcctagt tctgaaccga tatcatcaat gatgactaaa tccgctgttt tgatatctgc
     8281 aatcaacgat ccctcaatcg tttttctgag ttcaggattg ttgtacgaaa atttgatttg
     8341 atccagcatt tcctgtaacc cgataaataa gattttttta tcgtagtttg atcgcttcaa
     8401 gacttcccaa gccgctgcca tcgctaggtg acttttacct gttccttgtt ttccagttaa
     8461 aacaaggtga cttggatttc ctaacaagac tgaattaaca aagcgtttgg ttacttcaac
     8521 cgcttgtctt gtttcttgat cgacaatttg ataattctgc aatgtgcaat caaacaacgc
     8581 ttgattaggc acaacagaac cacctttgaa aaaattgatt gctcttgctt tcaggctctc
     8641 gttgtaaatt cgttctgttt gtaagtcttc ctgaactcgc aatgctttat acccacattg
     8701 catacaggtt ggcttacaac gttctgagcc atcagggttc ttcgtacgcc atccatacaa
     8761 aggctgtccg cattcaggac attcgccacg ttggacaagc actttctgta tcagcttttc
     8821 catgatttcc ccaacagttt ccatgcctac gcctcctctc aaataggcaa ctcatctgtg
     8881 ctaaatttct cgtattcaag cgttttagtt tgttttgttt tataatcacg atttgcttgc
     8941 gctgccattg tgtcgtattt ttctcgcaat tttttagcag ataaaatatt cgatgcccaa
     9001 aacacattgt gctgactcca ttcaatcatt ccccgtacct ggttttcggt ccgcttgtcg
     9061 atctcgatca ttttccgaat gtcatctgcc caactttgca gattcggctt tttgatttcc
     9121 tgattctgac aaatctgttt gaataactcc tccgcgagaa tgtagtaaac tgagtcggtg
     9181 tcgtaaacac gctttttgcg tggttgcgac gatgatgttt tattattctt ttcattctta
     9241 taattctttt cattcttgtt tgtgtgcact tgttgttcac ttgttgtgcg tttgatgttc
     9301 atttgttgtt cactgctttg gtacaaagac cagtttttta ttgatataac gctgtatttc
     9361 gtagttgatt tgatgttcaa cattccgttt ttttcaaatt gttttagcca tctccataca
     9421 gaaccgctgt tcacttgatg ttcacgtttc acgccttcgt tcatctcaga tgtgatagca
     9481 tcgcgccctg tgacgaattc tccgctgttc acttgtatct cttttccatt aaaaagtatc
     9541 ttgcgattct catgactcgc tttcatcaaa cacaagttcc ataatttata catataagga
     9601 ttggtccaaa cgaatgaatt catcactttt cgatgtaatt tgacatatcc tgtgttcatt
     9661 cgtatttctc ctctagaaaa gcaagggaga aagctccctc attatttgtt taacggcgga
     9721 ttagatgcat caaataatcc agtttgtaca tcttcatttt cttcagatat aacctctgct
     9781 tcttttcttt caggaatatc ttcttcaact tctgtttcag caataatgct gccgtcttct
     9841 tgaacccttt ggactctctc atccgatgtg gtggcttctt gcatttcgat ggacaagatt
     9901 ccccatttag aaagaagatt tctcaaaaca gtttttcgtg ccattgcatt gtaatcagat
     9961 gcccacacac cacttaactt tgtcttatcg cgatctttat tgttagcaat ccgatgagct
    10021 tcgatttctt gtttggtcca atagacagtt tttttgaatc cattcagtaa ctcgaaatag
    10081 ccaacatatc caatgacttc atcagacgtt ctaccatttg gatcaaactc aaactcttct
    10141 gtcagtcggt tccagctttt tagttctcct tcgtaaactt cgatcacatt taatgctttg
    10201 tatttacctg atcgttgggc taattggata tatcctttat agccaagcat gaattgagct
    10261 ttcttttccc attttcctgt ttgcttgttt ttactattga atggaactaa atatgcataa
    10321 cctaaattct tatctagccc aagatttaat gttgcagcag ttaacgcacc actcatgata
    10381 gacatcggtt cactatctgc aagataactg tcattagata caagagtcat aacattcgac
    10441 ataaaagcat tagcattgtc atgaagcacc tcttcgaact tctttctcat tgttggtgta
    10501 ttcatcaaag ctttaagtcc taattgacct ggtgcaactt gtttctgtgg ctttgctgcc
    10561 aattgatttt ttaacgattc atttgttgcc atattatttg atctcccttt cggttagcct
    10621 tcttgattca gtaacgttat aaatctcttc atcatttgcg acatctggat atttctctgc
    10681 tagtttcttc gagttcatac gtctcgtacg gacaaatttc caactgatga tgtttttttg
    10741 agtgataccg atactggctt cacgtttacc tagctcgctg ataatctcgt tgtctacttg
    10801 acggatagct gattcaattt ctttcttagt ccgtttgagt tcgtttttct gttcaactag
    10861 ttcatcgaaa tgaattggta acgtcgtttg aacgtcttca acatctgcat acttctcttt
    10921 caagaaatca gctgtcgctt gactaccatc aataattggc tcgatacctc caagaacgtt
    10981 tgtttcccaa aactctacta attgttcagt gattgtatcg atcagctctt gatctcgttc
    11041 aatccgcttc cagataaatt tttgaccacc gattaagaca gcgatgtaac aatagtcttt
    11101 gtttagaacg ttcatgtaat gttgaacttg acagagatag ctaagcggta cctcttcgcc
    11161 ttcccattct ttaccgagaa attgattggc tgttttgcat tccagaatgg cattttctcc
    11221 cactacttcc cgatcaatat ttgctcttaa aaatggatgt aatggatgtt caaatacttg
    11281 gtttcttctg cgtacttttt tgcctgtacg ttcctgaaac tctttagcaa ccacttcttc
    11341 taaaacatta ccccaataag cgggctcact tcctgattct tcaagtacta cttgtcctgt
    11401 tttttctagc catagttgat aaggagattt ccatttattc aatcctaaaa ttgttccaac
    11461 atccgaacct ccgatgcctt tcttacggtc ttcaagccat tcttgatggc tcatttctaa
    11521 ggtagattta ctcatcttca tcctcctcat caattggtgc ttcatatggt ggtttagcat
    11581 aatcaggatc agttaaatag ttgtcaagat ttgctaactc gttcatgttt aacctctttt
    11641 ctgcttgcta acacgcaaag cacaagcatg gtaattgcga ataacaagcc aaagattaca
    11701 taacttttcg ctacaatcac taacgtgaaa aataaaataa tgagtagatc aattgttttt
    11761 tcattcatca ttgaaattcc cccttgagaa tacgagtcat cacatcagct aaatcttcct
    11821 tgttattaat aacaaaagta tgatttgaag gttgattttc ttcttcattt ttcgcttgtt
    11881 cgtaagcatc cacagcaatt ctacgcataa tatgtgctga caatgggaga ttttcatcta
    11941 aatgttcttc ttgcatagca agacaaagta aaatttctgt tgtctcacca taaacgagac
    12001 ttttaagttc cccttctttt tgggctgtac aaataataga aacaccttgc ttttggcatt
    12061 cacgtttcaa ttcttcgata agattttcaa ttttcttatt catgtgatat aattctccta
    12121 gttttataaa tttgtatgtg actctttgct ggctggcaga gtcacttttt tatttgttgc
    12181 catgcttttt gcttttcgat atgttgcttg cttaaaataa tagggcggct atttccccac
    12241 cgattatcag caatcacttt accgattttt agcgcttctt ctcgtgccat agttgctcct
    12301 ttcttttgaa tcaagcagat tgattaaaac catcaatgct gcgaacaagc ttcccccgat
    12361 aatactttgg tgcgctacaa tcactaataa ccccaaaatg aatcctataa aaagtgtgtc
    12421 tgtcttcttc ataatctaat ctccctattt tttatttcta gcattctcaa atcctcaagt
    12481 tcagaagcaa ttagttcagc ttgcctatct gatagctcat cggcttttct aagcgctgca
    12541 cggtcatctt gtaattgttt cctgcgttgt ttaatcaaac cgagaatttg atgttcttgt
    12601 tgcaaggtat atgccatatc acttctcctt tcgtttattg aatttctcca tttctatttg
    12661 ataaaatatt tctatagaaa tggaggtgaa aactatggga aaaaatcaac atgttgtacc
    12721 taattccaat ggtggttgga atgtcaaagg tgctggaaat tctaaagcga ctgcacatac
    12781 taatacaaaa tcagaagcag taaaaatcgc tagagagatt tctaaaaatc aaggatctga
    12841 gctatttatt catggcagag atggaaaaat tcaaagccgt gatagtcacg gaaacgatcc
    12901 acacccacca aaaggttaat catatttagg agttaatcga atacgataac cgtcagcagg
    12961 agttgcatcg gtcaatgtaa cttctgctac ttttttccct tcttctgttt caataattaa
    13021 tcttgtgtaa ttgttctcat tcaaaatccc tatttttgat tcttccttca tgtcatccct
    13081 ccttcgtctc tcgccgtcac cacctccaag aagaggagtt gccaaaatga aaaaacgtgg
    13141 actttggcaa acttgaaagg agagcagcaa ctcctctttt cagaagtggt gaatttagat
    13201 tttttagcta ttaacaaaca gctctaactc taaatcgatt tttagaacgc catgttctaa
    13261 actgttcata taaatcgaca tcaattgtta tttctttgga agtaactgcg ttatacccct
    13321 cactaaaacg tgatttttta aattccttca ttcttctttg ataagtactt tcagaatact
    13381 tatatttctc tttaaactcc gctttgctca aacgttgtac catttttacg cctcctgttc
    13441 tgatggctga tcattagaat ctacttctaa cttgatatcc aaaagttctt cgatttgttt
    13501 taaacgttcc ttaggttttc ttttttcgat gaagatatct cgtaaataag taattgatat
    13561 acccattcgt cgagctaact ctctttgact ccaacctttc tttactaaag caattaatac
    13621 ttgctgtttt aaagtcatgc ttcatacccc cttatcgaat attttctaat atttttgtct
    13681 aataagtttg acaaaaactg caaatatgct attatgtaag catacaaaat acagcgcaca
    13741 aaaagcgagc ttactgatgt ctggggagac tattagttta ttgcttttag tctttgtatg
    13801 ccgtaaatat cttgcttaca aaactagtat aactgcaaat tctctatttg taaatagata
    13861 atttgcagaa aaatactttt tttgtcggta ttttgaaagg aatgcatatc ctatgctatt
    13921 agaccgtata aaaatcttat taaaagaacg ttcaatgacg ttggcagaac ttgaaagaaa
    13981 gttaaatttc ggaaatggaa gcatgtctag atggaataaa agtactccat ctggagataa
    14041 aatacaaaaa gttgcagatt actttaatgt atcaactgat tatcttttag gacgtactga
    14101 taaacgccat tattatgatt taacagaaaa agataaacaa gatattgctt tacaagctga
    14161 aaaattactt gaaggcttga attcagatgc tgaaacaaat tattatggtg aacctttaga
    14221 cgatgaagat aaggaaaaat tatacgatgc cattcaactt gcacttgcct taaccaaagt
    14281 taaagcaaag aaaaaattca ctcctaaaaa atatcgtcaa taggtgggct ttggtatgca
    14341 tttaaacaaa tactggatat tagaagaaat agaaaaatta tttgtagagt ttggatctga
    14401 tgcgattaat ccatataaaa taatcaaata ttctgatata gatttaattg
//
LOCUS       DACDOA010000061        14057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDOA010000061 DACDOA010000000
VERSION     DACDOA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.061"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            279..1127
                     /locus_tag="IWI39_001796"
     CDS             279..1127
                     /locus_tag="IWI39_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208897.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="IWI39_001797"
     CDS             complement(1298..1639)
                     /locus_tag="IWI39_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9208898.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="IWI39_001798"
     CDS             1725..2432
                     /locus_tag="IWI39_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9208899.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="IWI39_001799"
     CDS             2419..2742
                     /locus_tag="IWI39_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9208900.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="IWI39_001800"
     CDS             complement(2831..3868)
                     /locus_tag="IWI39_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9208901.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="IWI39_001801"
     CDS             complement(4315..5298)
                     /locus_tag="IWI39_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9208902.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="IWI39_001802"
     CDS             complement(5714..6484)
                     /locus_tag="IWI39_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9208903.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="IWI39_001803"
     CDS             complement(6477..7412)
                     /locus_tag="IWI39_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9208904.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="IWI39_001804"
     CDS             complement(8006..9655)
                     /locus_tag="IWI39_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9208905.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="IWI39_001805"
     CDS             complement(9850..10746)
                     /locus_tag="IWI39_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9208906.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="IWI39_001806"
     CDS             complement(10739..11419)
                     /locus_tag="IWI39_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9208907.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="IWI39_001807"
     CDS             complement(11420..12202)
                     /locus_tag="IWI39_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9208908.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="IWI39_001808"
     CDS             complement(12195..13112)
                     /locus_tag="IWI39_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9208909.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="IWI39_001809"
     CDS             complement(13115..13399)
                     /locus_tag="IWI39_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9208910.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4162 a         3012 c         2457 g         4426 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggttaa acaaaactct
    13921 attttattgc attttacagg aatgtatttc atgcgaaatt aataagtctt tgtttataga
    13981 gttttactag ggtataaaaa gttgtatgtt atcattacct caaaacatta cctcagcttg
    14041 gcgagttggt tgattga
//
LOCUS       DACDOA010000062        13807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDOA010000062 DACDOA010000000
VERSION     DACDOA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.062"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWI39_001810"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWI39_001810"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9208911.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWI39_001811"
     CDS             complement(2260..2511)
                     /locus_tag="IWI39_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9208912.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWI39_001812"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWI39_001812"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9208913.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWI39_001813"
     CDS             3461..3679
                     /locus_tag="IWI39_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208914.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IWI39_001814"
     CDS             complement(3968..4480)
                     /locus_tag="IWI39_001814"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9208915.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWI39_001815"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWI39_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9208916.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IWI39_001816"
     CDS             complement(6933..7367)
                     /locus_tag="IWI39_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9208917.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IWI39_001817"
     CDS             complement(7377..8165)
                     /locus_tag="IWI39_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9208918.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IWI39_001818"
     CDS             complement(8162..9103)
                     /locus_tag="IWI39_001818"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9208919.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IWI39_001819"
     CDS             complement(9280..9867)
                     /locus_tag="IWI39_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9208920.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWI39_001820"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWI39_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9208921.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IWI39_001821"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IWI39_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9208922.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IWI39_001822"
     CDS             complement(12784..13719)
                     /locus_tag="IWI39_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9208923.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACDOA010000063        13765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDOA010000063 DACDOA010000000
VERSION     DACDOA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.063"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..876
                     /locus_tag="IWI39_001823"
     CDS             <1..876
                     /locus_tag="IWI39_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9208924.1"
                     /translation="QMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLI
                     KSWNSLKQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTF
                     NNLVNGAQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKS
                     VQDFVGGIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADF
                     IAELFNANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDD
                     ISQAMGEGADINLEF"
     gene            909..1676
                     /locus_tag="IWI39_001824"
     CDS             909..1676
                     /locus_tag="IWI39_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9208925.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            1673..4504
                     /locus_tag="IWI39_001825"
     CDS             1673..4504
                     /locus_tag="IWI39_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9208926.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKASDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            4479..6236
                     /locus_tag="IWI39_001826"
     CDS             4479..6236
                     /locus_tag="IWI39_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP9208927.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            6253..7035
                     /locus_tag="IWI39_001827"
     CDS             6253..7035
                     /locus_tag="IWI39_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208928.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            7035..9221
                     /locus_tag="IWI39_001828"
     CDS             7035..9221
                     /locus_tag="IWI39_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP9208929.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            9224..9670
                     /locus_tag="IWI39_001829"
     CDS             9224..9670
                     /locus_tag="IWI39_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208930.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            9672..9809
                     /locus_tag="IWI39_001830"
     CDS             9672..9809
                     /locus_tag="IWI39_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9208931.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            9846..10139
                     /locus_tag="IWI39_001831"
     CDS             9846..10139
                     /locus_tag="IWI39_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208932.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            10162..10359
                     /locus_tag="IWI39_001832"
     CDS             10162..10359
                     /locus_tag="IWI39_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9208933.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            10370..11389
                     /locus_tag="IWI39_001833"
     CDS             10370..11389
                     /locus_tag="IWI39_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9208934.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            11646..12293
                     /locus_tag="IWI39_001834"
     CDS             11646..12293
                     /locus_tag="IWI39_001834"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9208935.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            12576..12649
                     /locus_tag="IWI39_001835"
     tRNA            12576..12649
                     /locus_tag="IWI39_001835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12610..12612,aa:Ile,seq:cat)
     gene            13195..>13765
                     /locus_tag="IWI39_001836"
     CDS             13195..>13765
                     /locus_tag="IWI39_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9208936.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         4754 a         2291 c         2936 g         3784 t
ORIGIN      
        1 caaatgtgga taaatctaaa attctccata attcaaactt ggattgatac aaaatatggt
       61 gcaattgaac tttggaataa tctaaaacaa tggtttttcc aaacggttaa taatatcgtg
      121 caaactctta taaaaagttg gaacagctta aagcaaggaa cgatagattt attcaacaat
      181 acagttcaag gtgctaaaga catttggact tcattcaaat cttggattgg tgatttaatt
      241 actggaacca aagataacat cattcaggga tgggaaaacc taaaacaagg tactatagat
      301 actttcaaca atttagtaaa tggtgctcaa gaggcatggg ataatttagt aaatgctgtt
      361 agtgacacgg ttgatagagt aactggctgg tttgataact tgaaaaatat cgatttacta
      421 gcagccggaa aagctatcat ggatagtttt ctagaagggt tacaaaatgc atggaaatct
      481 gtgcaagatt ttgttggagg tattggtgat tggattcgtg aacacaaagg acctatccaa
      541 tacgatagaa agctattgat tccagctggt caggctatta tgaacggtct gaataaaggt
      601 ctgacaggag gattcaatga cgtacagaat actgttggaa gtatggcaga ctttatcgcg
      661 gaacttttca atgcaaattc tgatgtagat atagctgcaa atttgaaaaa tgcaaataaa
      721 aacattggtg cacaagttga acataaaata aatatgggtg gctctactaa accagctgta
      781 tttaaaatca atcttggaag acaatcgttt agattgttta tggacgatat ttcacaagct
      841 atgggcgaag gtgcagatat taatctggaa ttttaggagg gaatattttg gatcaacgag
      901 aaaataaaat gtactcattc aaagatacaa ccattaatct cactagttct aaacgattcc
      961 ttccgacgtc tgccatgatg tacgatggaa tgtatttaga agatttgatt gatggttatc
     1021 aaacacttac ggtggaaggt agagaaatgc tttctgtaga agttgaacag caagagatac
     1081 aaattggttc aatcattaca aatcagaaaa taccttcaag aacactaaaa ataacatata
     1141 agctggaaga tagagatcca gaaaaactac agtttaaatt caaagaactg ttgaattatt
     1201 tataccggaa tgaagacgtg gaaattaggt ttcatgatga attagattat tattactacg
     1261 gtcgctatac atcaactgat actgttccag gagactccaa ctcgattatt tcgagtttta
     1321 atgtattctg tgcggatcca ctaaagtata cgaaagagtg tgttagtgat ggctatattg
     1381 gaaatccgat acagtttcct ataacaccaa gaaaaattga agttacttta tccatgaata
     1441 attcaatcaa aattacaaac ggagaacaaa atatcacgat aactgacgcg gcaataaaaa
     1501 caggagacgt gttggttttt gatttttccg atgagcaggt aactgtaaac ggagaagatt
     1561 gtacttctat gattgattta gaaagtgatt ttgagaactt ttatcttaag caaggtcaga
     1621 agataactag caataatggg aagcttaaaa tattctatag gggggcgaca atttgagtga
     1681 gacagtttat ttctttgatc acttgcaaaa acttattaaa agaaaaaata caagaagttt
     1741 gattgaagtc tcccaagaaa aagaaattag ttctgataag agtgatctaa tgaaagatac
     1801 tctttacgtt acgacaaaat atgataaaga aatagaggat gcaagatata tggcgattcg
     1861 tgaaaacgag tcgtcttttt cgttgtatcg aattactaaa gctagcgacc catttgaaac
     1921 attagagttt acagggttag gatttgcgac aaatgaatta gatgcttaca tcatcaaaga
     1981 tattaggccg agtgggcagc ccttaaaaaa tgttcttgat cgattgattg aatttactga
     2041 aggaaattgg cgcgttggtc acgtagaagc aatgttacca acagtaactg caacttttta
     2101 ctatgtctct gtaaaagaag cgttgaaaga attgcaaacc ttaggtatgg aatttgtctt
     2161 taggtgttct ttgaattctg atggaataaa ggataaatgg atcgaagtat atgaacaaat
     2221 tggcgaagaa tcgaatacac gtttcgtata tggtagtaaa gcattaacag ttgtaagaga
     2281 gatagataga agctcaatct caacttcaat gataggtcgt gggcgaggcg aagaggttgg
     2341 tgacggttac ggtagaagaa ttgaattcac tgatgttgaa tggaaaaagt cgaatggtga
     2401 tcctttagat aagcctaaag gccaaaattg gcttgaagat ccggaatcaa ctcaaaagta
     2461 tgggatacca caaaaagatg gatcaatgag aaaacgagaa accgtagtag tgtttgatga
     2521 tatagatgat ccaacagatt tacttaaaaa tacttattca accttaatcg attctgctag
     2581 accgttagta caattcaaag ctgaagtcac tggaggagat gtgataggaa atacagtgac
     2641 tattcacaga tacgataaag gttatcacta taaaactcgt atttataaaa ctacattcaa
     2701 tcggcttacc ggtcaaacga atatcgaact aggggataat ttaacacaag atgttagaaa
     2761 acaaacggct tctattgtca ataatattaa tagtttagaa tctagcaaaa tgacatttta
     2821 cgaatcgaca gagattggaa aatatcaaga tgacattatg cgaggtgcag gagataatgg
     2881 cggttctatt tattgggtaa atggaattga agctggtgtt agtgatagta gagaaatcta
     2941 tgaaactgtt tatatggatg gacctaacat tcctagatca cgctttttta tggtccaaaa
     3001 taactcaggg atatctttca aacaatgtaa aaaaggtgag tggcaaacaa ttcaagatgt
     3061 acacaatggt gatagcacga ccgcttggac gttggatgga actttcaatg ctaattttat
     3121 tcgcgcaggc attctgtctg gtatcctcgt gcaaggggta gctttaaaaa catttgacga
     3181 taaagatttc caattagtgg cagagggggg agaactttct tttgaaaaac aagtcaagtc
     3241 tactggacta gatgatgtac atggagaacg cttgggctca attgtatcta cttatggttc
     3301 taaagggatt aacggctttg ctgtctggaa agagccaaac tatatttttt ccattaatgc
     3361 tggggacggt ggcgatcgag ggaatcctat ttttcaaatt ccagcagatg ttactgctga
     3421 taagcgcaaa tataatcttt atggtgacgg caatttttca gaaggaaata taaccataga
     3481 tggacggcta gatgtaaaag aactctatgt taacggtaca aaaattgaca ctaatggagg
     3541 aggcaattcc ggagggaacg ataacggttg gaacggacaa tacccaccag aagtaactac
     3601 tgatcgggat aaacgatatt ggcaaatttg ggcaacggca ataggtgccg gttttacaca
     3661 acaagctgct gcagccttac ttggaaatgc acaaggagaa tcagatgcta atccaaccgc
     3721 tgatgagggc aatggcgcac cagggttcgg ttatggtgta tggcaatgga ccgattcttc
     3781 tggcgcaact agcggacgtg tctacatgat caatttaatg acaaaggctg gcatcagtga
     3841 tgatccagac acaatcacgg cacagtttaa attgttgatg tggcatgcac caaatggtca
     3901 atggatcgca actagcgctt atccttacac atggacacaa ttcatgaatc tgaccgatat
     3961 caacacagca gcacaagcat tcgtggctaa ctttgaacgt ccacgtgatc cacatccaga
     4021 acggacggca tggtcacaag aatggtatga caagttcaaa gatttggaaa ttcctgtatc
     4081 aaaaggatat atgaagccga ttgcggatcc aatcacagtg acgagcgaat ttggctggcg
     4141 cacttctcca atcacaggag cacaagaatt tcataacggt attgaccttg taaatggaaa
     4201 tcctaataca cctatttttg cttcagcgga tggcgaagtg attgttgcag gtgatgcaaa
     4261 ttactttgac tggtatggaa attggacagt gattaaacac gctgatggaa tgtatacagg
     4321 atacgcgcat caaagccgag tagacgtctc aaaaggtcag aaggtaactg ctggtcagca
     4381 aattggactg atggggacaa caggaccatc cactggagaa catcttcatt ttcaatttat
     4441 ggatgagttt tatccatctt cttcaggtca tttccacaat gcaagagact acatcgattt
     4501 ctaaaggagg gatagtcatg gcagaaacgc agcataaaat ggtcctatcc accaccgaac
     4561 caaacaacgg aataaatttg gttcgaattc ggcaagggga tgttttaacg caaaagttcg
     4621 ttgttgaagt ggtggaacat ggcaaactaa aaacattcga gggcctagtg ccgtttttta
     4681 ttaatacaac aaaatttggc gaaaaccaac ctgttgaaca aaaagtacaa gaatacagtc
     4741 cagcgcaagc aaggcttgtt tacaccttaa gtgagcctga ctggcaatgg ggtggtgaaa
     4801 acaccgcaca tttcagtttc cgatcactta atggtgatgg aacttggagt gaacaattta
     4861 gcacacagga ttttacctat cgagtcattt ctggaatatc tagaagccag ttacgtgact
     4921 ctggctatgt gtggacattt gaggatttgc taagaaaatt caaagattac atggatcagg
     4981 gcaaaaatga ctgggagcag tggttagaag ataatcgtga aatactggaa aatatcgatc
     5041 caggtggtat gatcattaac attctgaatg aagctaaagg cgactacgaa tcattagctg
     5101 atcgtttaaa ccaaaaatac caagtgccag tcggcagctc acaaattaga gaaacaacac
     5161 gcttttttga ttacgacacg atgaaatacg ttgacctagt gccgcgcaat ttgaatacgg
     5221 ttgtcaacag tgttaataac agcaaattta acttttcttt cattacagac attcacgcag
     5281 ataatcacaa cttgcgtata gatggcgtcg gttacaaaga tgcttcttat ttaagacatt
     5341 ggcgcgcaat ccctcaattt caaaaattag ggaacaaaac agatgtgatg atttacggcg
     5401 gagacaatat cgacggcgga cttggttcac tcggcagcga tataggcatt attgatgaat
     5461 ggagcgcgcg acattccatg ttaggcacgc tcaaacgctt cactaacgca gcggtagcag
     5521 gacaagaaaa accggttatt atctgcaaag ggaatcacga cgcttgtttc gaccccgcgt
     5581 ggcgcaagcg aaagggaatg ttatgcaacg ctgacttcga agagtattgg aacggtttgt
     5641 acggtggcgt gttgttccca gataaaaacg cagcaattta ccgtttcgat acttgtgatt
     5701 tttatgaagg cggcacaggc gacaagtacg tggacggtta cagcgataca gcgccgggag
     5761 ctttcagtgc caaacaaatt aacgctttcg gagaatggtt agtgaacgtt ccgagaaact
     5821 atcatgtggc gttagtagga catacgcctt taggtctcag caagtttccc gttcgcaatg
     5881 aaaacatgat tagcacgttg atagaagggt ttaaatctgg tagtccggta actatcgatt
     5941 ggtctaaatt aggacaaccg aatgacggtt cgttcggcgg attaaagacc ttcgcaatga
     6001 atacaaaagg cgccggcgtt gtagtaggtt atttctgcgg gcattggcac gaacaagtcg
     6061 aaggggcgtt tggaacagta aaaatgattc tttgcgacgt cggcttctct catacagcta
     6121 gccaagttga tacaccggat gaattagcgt tttataaaat agaagttgac acagcaacaa
     6181 gaaaagtgac aagcaaaggc gtagggcgcg cgagagactt cacttataac tattaaggcg
     6241 gtgaacaatt aaatgttaga ttttcaaagc aaaccaaata tttttgaaga aatgagttat
     6301 gaagaggctg tgaaatggtt attgcgtcaa gcggctatcc attacgacgg ctcggatcac
     6361 gacgcgcacg tcctagctac tgaaagcaac gcgggctttg ctacaccgga aacagttatg
     6421 caagcgcgtg ggcgttggtt acgcgattat aagctaccac aaaaatatcc gaacatttta
     6481 gacattccgc ccggcaaata cgcaaccaaa gccggatggg gcgcagacaa ccctgacggg
     6541 attgaagatg acagttttgt tgaaatgatg gtattcgcgg atcacgattc gagaaaatta
     6601 atcgtcgctt ttgctcgtta tagcggcgaa atttacatca aaatgacaca taacagcgaa
     6661 ccggtagagg gttataactc gttaggctgg cggcgcgttt acactacttc tgttcttttt
     6721 gaaggcgaat taagaaaagg acaatcagtc aatctgccag atgatacttt ccgttaccaa
     6781 acgcttcgta tccactacac agacggtgac ggagactttg tagaagaagt gaaacgtcaa
     6841 agatacgcgc gaattacaaa agcgaattta tggaatggag acgcgggagt gacgttcatc
     6901 gaatttgagc tgacaatcga agcgcgaaag ataacgatgt caaacggcag agctttagat
     6961 atttcatccg gcaatgtttc caatcccgca atgagcaaca acgtgaaaat aactaagatt
     7021 gaaggtgtga aataatggcg catgtcatca aaaaaggctc tatcaaagta cctacacagc
     7081 cgaaagacta cgatttgcaa gcaacggggc ttgtatttaa atcatacgat aatcaaatag
     7141 cgttagagtt caacgtcgaa caacagaacg gcacaccggc ggacttgcta ggagctaact
     7201 tgcgcttgtt gatgtttatc tatgatgaag tagatggaat gatcacgaaa gagccaatcc
     7261 cttttatcac gaaaaacctc atcactgaaa gcttcttgaa tggacaggtc gtatatatct
     7321 tgccagaagc gatgaaagct tataacggta tggtggaagc ttatgtttac atcgagtatc
     7381 cagacggatc aacaagtgat aacttaggct tcaccttccg tatgaagcgt tcagcaatcg
     7441 acggactagc gcaagataaa gcagactact ttattgcaga ctttcaacaa ttacttgatg
     7501 gagtcaaaca agaagcaaca gatgctgtta acgcagcatt aacaaatgtg gattctacga
     7561 ttgaaaaggc aaaccaacaa ataaatgaat ttgtagaggg agccacacaa gcaattgatc
     7621 aaactgttga cgaggtaaca gagcaactac aagctactca aactaagatt gataccgttt
     7681 ctcaaaacgt tacatcggca caaaacaatc ttaaagcagt tgaagacaag atgaatcaaa
     7741 ccaatcagca aatcggcgac ctcggcaagc tgaaaaagat gtacagtaac agcatcgact
     7801 tcgggaatta tgattatagt gggaatccga atttaatgtc caaactaaaa tcgagcgatt
     7861 ttaacgttgg ttaccacggg tcactaactt cggataacga aaagctacat tttacttctg
     7921 atggtacagg aagcattatt atgtttacgc gtattaatac acctcagctt gctagtggga
     7981 aaacctatac tctgagtgcg aaagttcgat ttgatgaagg aactacagga gctattgata
     8041 aattacgttt ggtgtatcgt acatcaccag gagaaaagat attattggaa gcaaatagta
     8101 caaatattac aacagatgat gtagggaaag aaataacaat caaaggtaca gctaacgtta
     8161 attatcaaat cacaaattta gatcgatttt atatgagtat tagctttgtt gacagggata
     8221 aaataaatgg cggatttaag ttgtacgaca tcaaaatcga agaaggctca acagccaccc
     8281 catatcagcc aaacttactt gatgaccctt actggctagg taaagcgcct ttgggtgaga
     8341 atattgctaa taagtctgtt acgtttccaa tcaaatctag cgcctacagc ctataccaag
     8401 ctaacatgga agaagaattt gttttaggtc aaacctatac aattaccatg aaggcaacca
     8461 aaccacctat ccaaacgttc attgtttaca acgaggatag tggagattat agatatggta
     8521 acttagagcc agtagaaggg ttagttgata catggggtct gacttttaca ccgcagaagg
     8581 ttggagttaa ctaccccaaa cggttaacta ttatccagta tccccaatca acaacaggtg
     8641 catgtcaaat tgactggctc aagatcgaaa aaggcgacac acgaaccccg aatattagtc
     8701 agtttaaata ctttggtgaa ggcttgaaag acagtaacaa tcccaatgat tacagttggg
     8761 atgtcacacc tgaatatact gaaaaaggct tgaatgatgc tgttaatgtg tatgatcctc
     8821 agagagttga aggtttgaaa aactttgccg atggtattca aatagcagga gataaagtga
     8881 tcagtgaaaa tgattgcact gtttatacat taactaaaga caacagtcaa tcgtttatcg
     8941 atgggtatgc aacatttatt aaacacggaa aagaagttat tgtaaatggt acagtaaagt
     9001 tcaaaaaagc ttatgctttt ggtgtaccac ttgatgatga agtcccagat gaatttgtcg
     9061 caaaaattgt tcatggcatg cttacaggtc cgtcaggaac aagtagtgta tcaaaagcga
     9121 tgtacgtgcg aaaagactta ggaacaatta ttacgaatag cgaatttgct gcaaatgaat
     9181 ggtttacatt ccatggtaat tattgggtag gggaggaata aaaatgaaaa atatttggaa
     9241 atacggacga acaggtggag agtatgctgg acaagtgctt gatgatatgg ttatgactgt
     9301 tccatttacc gatgttccac cactcgaagg aattcgtgct gatggggaac cactaacgat
     9361 tgctgatcag atgtttgatc ctaaactgaa ccaatggatt gttttagtga atgtactaga
     9421 tcacaacgat ttaaacaatc tcaaagcgat gtatgagtcg ttagaaaatg agaacggcga
     9481 tttaaaacag ctcaacgcca aactcatgct aagcgatgta gcgattaaac aggaaaatac
     9541 tgcattgaaa gaaaaagcgg atggtttagc acaaatcaat tcaaaaacga tgcttgcttc
     9601 gttccaaaat agcaaagaca ttgcagaaat taaagagcaa ctaaatccag cttcaaaggg
     9661 aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta
     9721 ctgacgatca agttcgacta ttcgttccac tatgcattac agacgaagaa gcagaaaaaa
     9781 tcattaataa agataagagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt
     9841 gtcacatgat taatttagga gaatggggaa caatcgcagg atcaatcact gcgattgttt
     9901 ctttgatttt attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc
     9961 tttcaaaagt aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag
    10021 atcgattgat gattcatgaa gaactaaaga aacacgatga aagattagat acacatacag
    10081 aaaaattggt agaacacaca caacaaatca aaactttatt tagagaaaga tctcggtaaa
    10141 aaagaaagga gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg
    10201 acagttttaa cagtattgcc agctgcatct gtattagttg caacactagg caaagcgtat
    10261 ggatggaatg gaacagatat gacagtactc actatcaatg cagtagcaac atttttaggc
    10321 gttatcactg gcgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa
    10381 aattactatt actgcgatga gcctattaac ggctcttttt ttattgccaa ttaatgggtt
    10441 tgcctatacg attaacaatg aatttaattt aggcccaaac gaaggtagct cacaagtagc
    10501 aaataatcag tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga
    10561 agcgcagtat atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg
    10621 cggaattgtt tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc
    10681 taatgcaaat agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga
    10741 gaaaaactac aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc
    10801 acttacacta gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca
    10861 aaatatttgg ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa
    10921 agaaaaatta gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga
    10981 tgataaacca gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg
    11041 aacaaattac gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc
    11101 tggttggcac atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg
    11161 gaaagaatta gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta
    11221 taatacttta ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa
    11281 gaaagtctat gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc
    11341 acaagatttt catgataaac gctggtattt aaatattccg caacgataaa aatagcccct
    11401 cgtttttgag gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa
    11461 aatgcggtaa tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa
    11521 tagaagcagc atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc
    11581 ctaagtataa atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa
    11641 gatgcatgcc taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt
    11701 ttggggtgaa cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata
    11761 aagataattc gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta
    11821 attatgtatc tgtaggaaaa atattagaag ttgctagtat taaacaattt ataagccaag
    11881 ctgataaatt aaaagagaaa cttaggataa ttattttact ttcattaaat agaggcatca
    11941 ttgatgaaag cgatttgaat agttttatta aagatattga agctaaaggt gaaaagcttt
    12001 cagtggaaga agtagaaaaa cgagctgtaa aacataattt ggtttgcgag acaagggaac
    12061 aagtaattaa agaaataaaa tcattatctg aagatatggt aaaatatcag aaggtgttta
    12121 attgctacaa gaaatataca caagaatcct atgtaatgcc acttaatatc caaacgatta
    12181 gtaaaaatag aattagaaga attaacaagt ttgatccttc tggaaatatg aaggcacctg
    12241 gaaatgtcct caatgaagta gatgaaagtt taaaaaaata ttttacaaag taaacgatat
    12301 ttttgttgac atttttctgt tcgttagtta ccatgtagtt atagaaggac gtaagcgcct
    12361 atttttaatg tacacatcgg gacgtatgcg cccacataga aacatagttg agagacatct
    12421 atatggtgtc tcttttttta ttaatatgca aaaaagtcta gttttttcaa taatatttat
    12481 gtaataataa attagccacc acaacaaaga atgaaaccca ttattatcta gtctatgtcc
    12541 attctttttg tttgcagtag ttgtgatggc tttccgtact cttagctcag ttggttagag
    12601 cagacggctc ataaccgtcc ggtcgtaggc tcgagtccta cagggtacat actctaagtg
    12661 atttaaattg cgaataaaat ataaaaatga ctcccctttt gactccccca agcagtatat
    12721 ggatgtctct aaaagttgtt tttttagata ataaaaagct gataataagc gaaaaacact
    12781 taaaatcagc tttaaaccct attctcgaaa tccggcgaac cggctaatac cggcgcgcag
    12841 gttcaaatcc tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca
    12901 tcaagtaata agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct
    12961 tattaccgca gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga
    13021 cttttgtcac agccccttat tgttgtactt ttaacctaca atattttttg attccccctg
    13081 agtactattt tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa
    13141 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt
    13201 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
    13261 tcttataatg aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta
    13321 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
    13381 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
    13441 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
    13501 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
    13561 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
    13621 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
    13681 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
    13741 aattatcgct cgctatcaaa tggaa
//
LOCUS       DACDOA010000064        13537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDOA010000064 DACDOA010000000
VERSION     DACDOA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.064"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            176..1363
                     /locus_tag="IWI39_001837"
     CDS             176..1363
                     /locus_tag="IWI39_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9208937.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1455..1820)
                     /locus_tag="IWI39_001838"
     CDS             complement(1455..1820)
                     /locus_tag="IWI39_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9208938.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1817..2182)
                     /locus_tag="IWI39_001839"
     CDS             complement(1817..2182)
                     /locus_tag="IWI39_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9208939.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2374..2622)
                     /locus_tag="IWI39_001840"
     CDS             complement(2374..2622)
                     /locus_tag="IWI39_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208940.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2621..2749
                     /locus_tag="IWI39_001841"
     CDS             2621..2749
                     /locus_tag="IWI39_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9208941.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2806..4500
                     /locus_tag="IWI39_001842"
     CDS             2806..4500
                     /locus_tag="IWI39_001842"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9208942.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4504..4623
                     /locus_tag="IWI39_001843"
                     /pseudo
     CDS             4504..4623
                     /locus_tag="IWI39_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4705..4896
                     /locus_tag="IWI39_001844"
     CDS             4705..4896
                     /locus_tag="IWI39_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208943.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            5274..6407
                     /locus_tag="IWI39_001845"
     CDS             5274..6407
                     /locus_tag="IWI39_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9208944.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6438..6758
                     /locus_tag="IWI39_001846"
     CDS             6438..6758
                     /locus_tag="IWI39_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208945.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6787..7251)
                     /locus_tag="IWI39_001847"
     CDS             complement(6787..7251)
                     /locus_tag="IWI39_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9208946.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7382..8635)
                     /locus_tag="IWI39_001848"
     CDS             complement(7382..8635)
                     /locus_tag="IWI39_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9208947.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8664..8981)
                     /locus_tag="IWI39_001849"
     CDS             complement(8664..8981)
                     /locus_tag="IWI39_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9208948.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9007..9327)
                     /locus_tag="IWI39_001850"
     CDS             complement(9007..9327)
                     /locus_tag="IWI39_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9208949.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9791..11206)
                     /locus_tag="IWI39_001851"
     CDS             complement(9791..11206)
                     /locus_tag="IWI39_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9208950.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11223..12539)
                     /locus_tag="IWI39_001852"
     CDS             complement(11223..12539)
                     /locus_tag="IWI39_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9208951.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12689..13387)
                     /locus_tag="IWI39_001853"
     CDS             complement(12689..13387)
                     /locus_tag="IWI39_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9208952.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4290 a         2763 c         2330 g         4154 t
ORIGIN      
        1 aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca aaaccacttt
       61 actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt atacacatcg
      121 ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca agatcatgca
      181 gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg aagaagatga
      241 acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag cttattcatt
      301 aaaaaaacaa gtattagctt attttaatga ctgcgggatc gaaatcgacc gctactcccc
      361 tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc gtttaggcgg
      421 ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt tcattgaatc
      481 cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct tatcgattgg
      541 ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt caaaatttat
      601 cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg aaaacactga
      661 tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat tcaatctttg
      721 taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc tacaccaagt
      781 gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat ctcacttcaa
      841 tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga tgcgttcggc
      901 ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg aggaacagac
      961 aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt taccttatga
     1021 tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg gaattctccg
     1081 tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc attatctgat
     1141 ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac caacaattta
     1201 ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg tattatgtga
     1261 acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta tttcgatcaa
     1321 tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg tgacaaaaat
     1381 catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc ttgatacacg
     1441 tcaggctcac aacctcactt attttggaca tctacgggtc cgatctcttt atttaaaaaa
     1501 taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc ctgcaaggta
     1561 ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact tagaagtata
     1621 tagcctccca acatcttttt aatagcattg ttctcgctga ctcggtcgct ttgcactttt
     1681 gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc ccaaaccaat
     1741 aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg aagaagaaac
     1801 tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg tcttctattt
     1861 cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa
     1921 tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact agtacttcca
     1981 agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga gtattacgtt
     2041 ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc
     2101 cgacaattaa gataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt
     2161 caattaaatt tgagcgaaac atatacgcta cctactttct tttaatttgt tcctattctt
     2221 ttattgtaaa agaaagttac cttgttcgtc tatttaaaag ttgttctaat gataacggtt
     2281 cgcttaaatt tttctttttc actctcccat ttttctccct ccatccataa atcgtggaag
     2341 tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct tttcaaagaa
     2401 gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa atagaaccat acacctcctc
     2461 aaatcttttc ttcttaccta ccctattttt atttacagga aaatcagaca cttcttcttt
     2521 tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg cattctaata aaaacgaact
     2581 atccttggat atagaaagag aaaacaagga ggcgtttttc atgagtaaaa aattaatcaa
     2641 agtcggcatc ggtttaggtt tgcttgccct tggcgctgca tatctaggaa aaaagacagg
     2701 actattcgaa gatgacagtc atttatatga tgaattcgaa tcaatctagc atcctattta
     2761 gttgaatcac tacggacata cttggaagac attggaggaa tcacaatgag aaaaagtaaa
     2821 gcaataatga ttggtgccgg tattgccaat atggcagcgg ctgtttattt gattcaagaa
     2881 ggcaaatgga aaggggatca gatcaccttt tattcattgg atgatcacgg ttcaaatgat
     2941 ggtgcaccga ccgacactgt agtggatgaa tattggaata aaaaccatcc attagaaaac
     3001 caaaaaggct atattgctcg aggcggtcgc atgcttaatt accgtaccta tgtagatttg
     3061 atggatctgt taagtcgaat accttctgtt actgagccag ggatgactgc tgaagaagat
     3121 acgagagatt ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat ggaaggtggc
     3181 aaaggaatca ttgatggcgg aaaactggga ttcaataata aagaccgtat tcttctgact
     3241 aaactgattg ctatgcctga ttcagaagaa gaaaaactag acaatatcac tatcgaagaa
     3301 tatttcaaag atgatccaca cttttttgaa acaaacttct ggtttatgtg ggaaacaaca
     3361 tttgctttca gaacacgtag ttctgctcaa gaattgcgtc gctatatgca tcaaatgatt
     3421 tatgaattca ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta caatcaatac
     3481 gaaagtatta tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa aatcattttg
     3541 aaccgccgtg tcactgactg ggaattcaaa gaaacaccga tgcaagatga aatcactgtg
     3601 actggtttga aaattaccaa tacactgact caagaagaag aatacgttgt ggtcgatgaa
     3661 gatacagcag ttattttcac taatgggtcc attacagatt cagctacttt aggagattac
     3721 gaaacaccag ctgtagaaaa tatggattat ggtgcagctt ctagtctctg gaaaaaagcc
     3781 agtgaacgtt tctacaactt gggaaatcct gacaaattct ttgctgatcg agatgcaagc
     3841 gaatgggtca gctttaccct gacgacgaaa aaccatttgt tgttgaatga aatcacccgt
     3901 atcacgactc aagaaccagg aaatgccttg aattcattta tttcaaccac accgatcacg
     3961 ccgttaggcc aaaaagatgt caatatgtct atcgtcgtgc atcatcaacc gcattttacg
     4021 tctcagaaac caaatgaaac agttatttgg ggttatttcc tttatccaag acgacgtggc
     4081 gaatttgtgg ataaacagta tattaaaatg aatggaaaag aaatgctaga agaactgatt
     4141 ggacaacttt caaaagtaga tcctggacca gtgaatattc gtgaaaaaga aacagaaatt
     4201 ttcgacagcg taataaataa tatccctgtc tatatgcctt atgcttccgc gttgttcaat
     4261 aatcgtgcca aaagcgatcg accaaaagtc attcctaaac attcgaccaa tctggccttt
     4321 acaggagaat ttgtcgagca gccttatcag atgatcttta ctgaacaaag tgctgtacgt
     4381 tctggagaaa tcgctgcttt ccattttgcg ggtattccta tgtcccgtct tgtcaaaaca
     4441 ccgcgttacg acaaagatat tcctactcta gctcgtgccg caaaaaaaat gtttgaataa
     4501 tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccag gctaaaacag
     4561 cttcttatgt ttttgatttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     4621 ttaccaagag gttgtgaaaa agctgccctg catcaagtaa taagaacacc caagctaaaa
     4681 ctgcttctta tgtttttgac ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4741 catttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta
     4801 aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa
     4861 caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg
     4921 tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt
     4981 ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg
     5041 tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt
     5101 acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt
     5161 catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta
     5221 tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag
     5281 cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat
     5341 tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc
     5401 atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa
     5461 tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg
     5521 ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc
     5581 aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga
     5641 acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt
     5701 catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt
     5761 tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga
     5821 ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag
     5881 agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg
     5941 gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg
     6001 ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca
     6061 aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg
     6121 catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag
     6181 cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg
     6241 caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc
     6301 ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct
     6361 ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa
     6421 tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa
     6481 aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca
     6541 cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg
     6601 cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt
     6661 tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa
     6721 aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct
     6781 cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt
     6841 gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa
     6901 gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt
     6961 ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag
     7021 atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt
     7081 ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat
     7141 actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg
     7201 ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac
     7261 ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga
     7321 caacaatagt gatgtaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta
     7381 atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta
     7441 gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt
     7501 ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg
     7561 ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca
     7621 aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt
     7681 cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt
     7741 ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta
     7801 agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgcccagctg
     7861 cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga
     7921 ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta
     7981 aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag
     8041 aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag
     8101 agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac
     8161 catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg
     8221 aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc
     8281 ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc
     8341 cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac
     8401 cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca
     8461 attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca
     8521 ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa
     8581 tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc
     8641 ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatc aatgatctct
     8701 tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa
     8761 agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga
     8821 tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca
     8881 ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg
     8941 atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa
     9001 tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac cattcatcat
     9061 gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc
     9121 aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga
     9181 ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt
     9241 tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca
     9301 tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt
     9361 atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt
     9421 aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt
     9481 aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc
     9541 acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca
     9601 agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc
     9661 tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa
     9721 cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa
     9781 aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag
     9841 tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc
     9901 gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc
     9961 ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat
    10021 gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact
    10081 gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat
    10141 tttcttttag atacatggct atatcataca tgattttcgg atagatttcc catcccctgg
    10201 aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag
    10261 gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga
    10321 ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga
    10381 aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca
    10441 tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt
    10501 ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac
    10561 cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat
    10621 tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc
    10681 aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt
    10741 aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc
    10801 agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg
    10861 gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt
    10921 catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat
    10981 aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat
    11041 tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc
    11101 tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag
    11161 aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt
    11221 ttttagatcc catcattttc cacacgattt gctactttta caaatggcag atagatcaaa
    11281 atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc
    11341 agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca
    11401 aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac
    11461 ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg
    11521 ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca
    11581 actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac
    11641 atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc
    11701 gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt
    11761 ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt
    11821 aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg
    11881 tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga
    11941 atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt
    12001 ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag
    12061 accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct
    12121 actataacag tctgattttc attcactaat tcataaacag ttggattaac agataaataa
    12181 gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg
    12241 atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca
    12301 gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt
    12361 tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga
    12421 atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt
    12481 gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt
    12541 cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat
    12601 gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta
    12661 tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct attattttct
    12721 gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat
    12781 tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat
    12841 gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt gggataatat
    12901 gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt
    12961 gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca
    13021 tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca
    13081 ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa
    13141 aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc
    13201 gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc
    13261 ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact
    13321 gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt
    13381 cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt
    13441 ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa
    13501 cgaagaagat atttaccata aaaaagacta ggataaa
//
LOCUS       DACDOA010000065        13518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDOA010000065 DACDOA010000000
VERSION     DACDOA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.065"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..370
                     /locus_tag="IWI39_001854"
     CDS             2..370
                     /locus_tag="IWI39_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9208953.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="IWI39_001855"
     CDS             550..1491
                     /locus_tag="IWI39_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9208954.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="IWI39_001856"
     CDS             1560..2291
                     /locus_tag="IWI39_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9208955.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="IWI39_001857"
     CDS             2435..4615
                     /locus_tag="IWI39_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9208956.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="IWI39_001858"
     CDS             4635..5117
                     /locus_tag="IWI39_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9208957.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="IWI39_001859"
     CDS             5133..5894
                     /locus_tag="IWI39_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9208958.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="IWI39_001860"
     CDS             6025..7359
                     /locus_tag="IWI39_001860"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9208959.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="IWI39_001861"
     CDS             7666..8151
                     /locus_tag="IWI39_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9208960.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="IWI39_001862"
     CDS             8144..8827
                     /locus_tag="IWI39_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9208961.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="IWI39_001863"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="IWI39_001863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9208962.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="IWI39_001864"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="IWI39_001864"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9208963.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="IWI39_001865"
     CDS             12443..13072
                     /locus_tag="IWI39_001865"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9208964.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="IWI39_001866"
     CDS             13124..13456
                     /locus_tag="IWI39_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9208965.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4558 a         2381 c         2982 g         3597 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttt
//
LOCUS       DACDOA010000066        13504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDOA010000066 DACDOA010000000
VERSION     DACDOA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.066"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..812)
                     /locus_tag="IWI39_001867"
     CDS             complement(120..812)
                     /locus_tag="IWI39_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9208966.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            897..1073
                     /locus_tag="IWI39_001868"
                     /pseudo
     CDS             897..1073
                     /locus_tag="IWI39_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1249..1818)
                     /locus_tag="IWI39_001869"
     CDS             complement(1249..1818)
                     /locus_tag="IWI39_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9208967.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1892..2215)
                     /locus_tag="IWI39_001870"
     CDS             complement(1892..2215)
                     /locus_tag="IWI39_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208968.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2369..3181)
                     /locus_tag="IWI39_001871"
     CDS             complement(2369..3181)
                     /locus_tag="IWI39_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208969.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3535..4383
                     /locus_tag="IWI39_001872"
     CDS             3535..4383
                     /locus_tag="IWI39_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9208970.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4528..5469
                     /locus_tag="IWI39_001873"
     CDS             4528..5469
                     /locus_tag="IWI39_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9208971.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5459..7630
                     /locus_tag="IWI39_001874"
                     /pseudo
     CDS             5459..7630
                     /locus_tag="IWI39_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7742..9913)
                     /gene="copB"
                     /locus_tag="IWI39_001875"
     CDS             complement(7742..9913)
                     /gene="copB"
                     /locus_tag="IWI39_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9208972.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9933..12119)
                     /locus_tag="IWI39_001876"
     CDS             complement(9933..12119)
                     /locus_tag="IWI39_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9208973.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12119..12328)
                     /gene="copZ"
                     /locus_tag="IWI39_001877"
     CDS             complement(12119..12328)
                     /gene="copZ"
                     /locus_tag="IWI39_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9208974.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12341..12781)
                     /locus_tag="IWI39_001878"
     CDS             complement(12341..12781)
                     /locus_tag="IWI39_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9208975.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12855..13394)
                     /locus_tag="IWI39_001879"
     CDS             complement(12855..13394)
                     /locus_tag="IWI39_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9208976.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3797 a         2827 c         2402 g         4478 t
ORIGIN      
        1 tcaaagtgaa ctgaacccca aaagttgaac tatttaatgg actgtttccg atattctact
       61 ggagatagtc cattaaatag ttcaactttt ggggttcagt tcaaagaggg ggggattttt
      121 tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa aatcttgtgc tccaccctta
      181 gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt atactttctt attagggaaa
      241 tcacgcatat tgaaagatac atgatagcca acatttccta aagtattata agcttgattt
      301 acgtctggtc tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag
      361 tccataatga aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag
      421 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
      481 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
      541 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
      601 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      661 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
      721 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
      781 ttatcatgtg tatgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
      841 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
      901 cagctagaac gagacaagca ggaatacctt tagaagatat caaaggtttt ttaggtcata
      961 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
     1021 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
     1081 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
     1141 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
     1201 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
     1261 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
     1321 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
     1381 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
     1441 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     1501 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     1561 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     1621 cactgtttga cgaaggacat ctagttcttg caagagcgtt tcgtattgtt cttgggtcag
     1681 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
     1741 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
     1801 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
     1861 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
     1921 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
     1981 cgttatggtc tagtggaaaa aaatgatcga tgatcactag atctgcatac cgaggcggct
     2041 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
     2101 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
     2161 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
     2221 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
     2281 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
     2341 ggtcatggat accaattacg taacgaagtc attcttggta aagtactttt gtcagctttg
     2401 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
     2461 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
     2521 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
     2581 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
     2641 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
     2701 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag caaaacagca
     2761 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
     2821 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
     2881 aaggttcatt caatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
     2941 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
     3001 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
     3061 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
     3121 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
     3181 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa
     3241 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
     3301 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
     3361 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
     3421 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
     3481 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
     3541 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
     3601 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
     3661 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
     3721 tttgaatttc gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt
     3781 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
     3841 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
     3901 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
     3961 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
     4021 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
     4081 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     4141 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     4201 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     4261 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     4321 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     4381 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
     4441 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
     4501 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
     4561 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
     4621 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
     4681 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
     4741 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
     4801 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
     4861 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
     4921 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
     4981 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
     5041 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
     5101 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
     5161 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
     5221 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac
     5281 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
     5341 tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt atcttgttta tttcgtagaa
     5401 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
     5461 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
     5521 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
     5581 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
     5641 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
     5701 atggatctta cagttttttt cgtcagtcac agaggaattc ttccatctat cccaaggtaa
     5761 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
     5821 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
     5881 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
     5941 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg
     6001 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
     6061 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
     6121 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
     6181 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
     6241 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
     6301 aaatcattac accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca
     6361 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
     6421 attgacccta aaaaaacaag ttttcttacc ttatccttat ggaaatacta ccttgactga
     6481 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
     6541 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
     6601 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
     6661 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
     6721 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
     6781 tttccgctat tccaaaacgg ataccgggca tactccaaaa gaacacgatt atgtggatta
     6841 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
     6901 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
     6961 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
     7021 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
     7081 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
     7141 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
     7201 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
     7261 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
     7321 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
     7381 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
     7441 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
     7501 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga
     7561 aataacgcat ataataagaa aaatggtata caaaaaaatt ctgacaaaag ggagccgctt
     7621 caaaaaataa gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt
     7681 gagtatcgat tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt
     7741 attatctgat tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca
     7801 agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcta
     7861 gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc attttctttc
     7921 gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata
     7981 cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag
     8041 cacgggctaa acttggcgca tcattgatcc catcacccac catgattact tttttccctt
     8101 ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt
     8161 cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca
     8221 tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta
     8281 ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg
     8341 tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct
     8401 caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga
     8461 ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat
     8521 aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt
     8581 gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt
     8641 ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct
     8701 ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggaacgag
     8761 caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta
     8821 ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga
     8881 tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta
     8941 gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat
     9001 tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa
     9061 tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt
     9121 caacaattgt ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga
     9181 cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt
     9241 tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg
     9301 cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag
     9361 ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg
     9421 aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag
     9481 gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa
     9541 atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg
     9601 gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata
     9661 gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt
     9721 gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc
     9781 ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat
     9841 gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat
     9901 cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagcac
     9961 agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga
    10021 ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa
    10081 agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat
    10141 gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt
    10201 cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct
    10261 gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct
    10321 ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg
    10381 gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc
    10441 ttgattgctt gtttgacttc tagtttgatt tgatcagcca cggcaatcaa cccgatcact
    10501 tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt
    10561 tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa
    10621 tagcgtactc cgttaatcgt accgctgatt ccagcacctg ggtgggctgt aaaatcaatc
    10681 atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga
    10741 tgttcagaag cttgttctaa agtataaaaa atagatagga cttctttaga accgactaga
    10801 tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct
    10861 aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca
    10921 acacccgttc ctaccataat agctgttgga gtcgctaaac ctaatgcaca tggacacgca
    10981 atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct
    11041 gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc
    11101 cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg
    11161 atttgcgcta atactgtctg cttgccgact tgtgtaactt ccatgtgaag tagaccattc
    11221 gtattgattg tcccgccaaa gagcgtgtca tccggattct tttctacagg aagattttca
    11281 ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga
    11341 atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac
    11401 gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt
    11461 ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc
    11521 agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat
    11581 ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata
    11641 tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa
    11701 aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc
    11761 gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc
    11821 gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt
    11881 ttttgtttgt gggcttcatc gtacaaaatt gcaccatagc cgatgttttc tacgctttgg
    11941 attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg
    12001 actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa
    12061 tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc
    12121 aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa
    12181 cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt
    12241 gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca
    12301 taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt
    12361 cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt
    12421 gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact agatccgcaa
    12481 tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa
    12541 ctgtttccat ctccgagaca gctggataat aaatgaattt ttttccttct tgttctgtcc
    12601 agatcacttc tttttttacc aaacgaccca gcaaagtttt gatcgttgct ggtttccaat
    12661 ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa
    12721 taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca
    12781 tttatttcac acctttcgtt tacaaatgta atcattatct tatgaataca gattacactt
    12841 gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt
    12901 ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc
    12961 tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa aaattctaag
    13021 tattccctgt ttagtttctc cgaaaatgat tcaggcatat ctggccgaga ttttttgttt
    13081 atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta
    13141 agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt ataatttcct
    13201 tcaacgatcc atgacggatt agataccata aacaattgct gttcttgcaa aaaccacttt
    13261 tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag tagcggaaag
    13321 tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact ttgtccaatc
    13381 acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat cataccactt
    13441 ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc cttgtttcga
    13501 ataa
//
LOCUS       DACDOA010000067        13438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDOA010000067 DACDOA010000000
VERSION     DACDOA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.067"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..465)
                     /locus_tag="IWI39_001880"
     CDS             complement(<1..465)
                     /locus_tag="IWI39_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9208977.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSD"
     gene            complement(713..1549)
                     /locus_tag="IWI39_001881"
     CDS             complement(713..1549)
                     /locus_tag="IWI39_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9208978.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1560..2489)
                     /locus_tag="IWI39_001882"
     CDS             complement(1560..2489)
                     /locus_tag="IWI39_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9208979.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2681..3034
                     /locus_tag="IWI39_001883"
     CDS             2681..3034
                     /locus_tag="IWI39_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9208980.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3108..3764)
                     /locus_tag="IWI39_001884"
     CDS             complement(3108..3764)
                     /locus_tag="IWI39_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9208981.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3990..5924
                     /locus_tag="IWI39_001885"
     CDS             3990..5924
                     /locus_tag="IWI39_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9208982.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6010..7233)
                     /locus_tag="IWI39_001886"
     CDS             complement(6010..7233)
                     /locus_tag="IWI39_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9208983.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7227..9125)
                     /locus_tag="IWI39_001887"
     CDS             complement(7227..9125)
                     /locus_tag="IWI39_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9208984.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9119..10237)
                     /locus_tag="IWI39_001888"
     CDS             complement(9119..10237)
                     /locus_tag="IWI39_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9208985.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10320..11153)
                     /locus_tag="IWI39_001889"
     CDS             complement(10320..11153)
                     /locus_tag="IWI39_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9208986.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11146..12063)
                     /locus_tag="IWI39_001890"
     CDS             complement(11146..12063)
                     /locus_tag="IWI39_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9208987.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12290..13414)
                     /locus_tag="IWI39_001891"
     CDS             complement(12290..13414)
                     /locus_tag="IWI39_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9208988.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4196 a         2694 c         2212 g         4336 t
ORIGIN      
        1 atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt gaacaggata
       61 gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt cataagtgaa
      121 ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta ttgctttatt
      181 cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa gcatttcgcc
      241 tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa tatctgagga
      301 atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc cctgaacgat
      361 ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta ctagcatttt
      421 ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa gtacctccac
      481 tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt taggaataaa
      541 ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa tactttcagg
      601 atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa agccagacaa
      661 ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc ttttaaaaag
      721 aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta acaactacag
      781 atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct tctattttat
      841 accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa ccaaagactc
      901 ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct gtattgctat
      961 cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg aattcgctag
     1021 ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc ttgatagttt
     1081 gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct gatttgttga
     1141 aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc cctactgatt
     1201 ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg agactaactg
     1261 ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg attccacgtt
     1321 gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct tctgcttttc
     1381 cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa atcattgaaa
     1441 gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca gaaacttgca
     1501 agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata ttttccctcc
     1561 taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag cgataaaata
     1621 aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac cattgggcca
     1681 aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga agatcctata
     1741 aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat gatgataatg
     1801 gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata agtctgcatc
     1861 gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa ttcgtcgatt
     1921 gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc attcttctca
     1981 ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga cgtgattttt
     2041 tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac aagggcaaca
     2101 aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa tgcgataaaa
     2161 gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc aaacgctgtg
     2221 tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc gcattcacat
     2281 gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg attttcagga
     2341 agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga gataagacag
     2401 cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt actcatctgc
     2461 aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc tagttcattc
     2521 gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat ttttacacaa
     2581 ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat taaattaagg
     2641 tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa ccactaactt
     2701 gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc tttcgatgcc
     2761 taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca cacattatga
     2821 tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta aatttgaagt
     2881 gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc ggcaagcacg
     2941 gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag aagatgattc
     3001 cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga aaaaagcgat
     3061 tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta ttctgctgta
     3121 ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg caattcattc
     3181 gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc agcggtaaag
     3241 ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg tgctctttca
     3301 gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc tgacatatct
     3361 tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc attcacatca
     3421 aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag taaagcactt
     3481 cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt taatgtcttt
     3541 gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc taattcgcca
     3601 aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc acttaattca
     3661 gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg ataatacaaa
     3721 ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg acctccacgt
     3781 attgtctaat tacatctttg atttctccat catagcagta tttataggaa aaaatcaatt
     3841 ccattgctca gatattgtga aattttattc tttattcatt tatttatcat ttctgcaaat
     3901 ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt ttatttgagg
     3961 agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat cagattgctc
     4021 gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt acaggcgctc
     4081 gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag atcattgctg
     4141 gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact ctcggatacc
     4201 tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg ctgaaagctt
     4261 ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc attagcgaag
     4321 gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg ctgcaacacg
     4381 atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta ttgcatgggt
     4441 ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc ggacagcgca
     4501 cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt ctagatgaac
     4561 caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg caaagctata
     4621 ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta gtcaatgaag
     4681 tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg aaatatctag
     4741 atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag caaacagaaa
     4801 tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc acaaaacgag
     4861 cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct caaggggatg
     4921 aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta gtcttgcaaa
     4981 tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc aatctagata
     5041 tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa tcgacgttgc
     5101 tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta gggacaaatg
     5161 tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag acgatcctag
     5221 cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt gctttaggtg
     5281 ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt ggcggtgaaa
     5341 aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg attttggatg
     5401 aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca ttgatcgatt
     5461 atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg atcgcgacaa
     5521 aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat gattattatt
     5581 tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat gagccagaat
     5641 ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag caaaagctta
     5701 tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg atcgatgaat
     5761 ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat cacgtaaaac
     5821 tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca aaattgacgg
     5881 aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa aaccaaaaca
     5941 gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa aattccatat
     6001 ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag cttctaaata
     6061 atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct tattataaac
     6121 agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt tcaattgtcg
     6181 cataattttc caaacagcag actggtataa gttcttttct gttttgtcta atagacctga
     6241 ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat ctttttcttc
     6301 tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg gcaaccgatt
     6361 cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt tcaaataacg
     6421 ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac aggaattatc
     6481 actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat ccatataaaa
     6541 tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt gaatatgagc
     6601 ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat acaataatgg
     6661 aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac taggatctcc
     6721 ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt ctgcagcatc
     6781 aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta cggttgataa
     6841 agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa tcctgtggat
     6901 aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg tcttctcaaa
     6961 taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt ctcttaatgg
     7021 ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac tgatacgttt
     7081 gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt ctttttccat
     7141 taattcttta taacattcat ttatccattt ttctggatgt attatctctt caaacatcac
     7201 tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt attgttttta
     7261 ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt gttccatact
     7321 tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca atgggttcct
     7381 tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat caagtttgta
     7441 ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact taaacagata
     7501 atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc tatgattatt
     7561 ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac atattctgta
     7621 tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag ccaagttctg
     7681 atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt acatacaatg
     7741 tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc attcttaaaa
     7801 ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat tgccatttct
     7861 gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata agcaaatttt
     7921 aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc attcaaaaaa
     7981 aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag tttagttcct
     8041 tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc aggtagcgcc
     8101 aacaataaaa aaatcttatt cagccataat ggagataacg tactattata gggttcagtc
     8161 aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc aaatatttct
     8221 ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc taatttaaaa
     8281 ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa acgataaatc
     8341 atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat atagtctcta
     8401 gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg ttctagtacg
     8461 ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg gcgacctctt
     8521 gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc aaatgcttct
     8581 ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc gacaattatc
     8641 ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga gaaccaatga
     8701 ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc atgatgatat
     8761 aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg tatttctccc
     8821 caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat taatttcaac
     8881 catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg agagaatcct
     8941 tccattcctg caattttatt tccatatcct accccttgat gtatataatc aatttgtgcg
     9001 ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa gtcaaagtaa
     9061 tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc tctttttact
     9121 accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa aaataatcag
     9181 aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct ccgcctccaa
     9241 tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt tggagtatat
     9301 tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct gctaattcca
     9361 gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct tttacagcaa
     9421 aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt actgctatct
     9481 tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac gcgaatccat
     9541 ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct tgcccgccaa
     9601 gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg gttgatccat
     9661 ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt ttagagaacc
     9721 tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc atactatcaa
     9781 taatcatcca accaggttta tggtcattcc atgctactaa atagttccct gaaatattat
     9841 agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt ttgttttgag
     9901 actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca tgatgaacgt
     9961 ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct gcctctttga
    10021 acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa aaaccattgg
    10081 tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg taagaaatat
    10141 atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa gctacattca
    10201 aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca ttcttttaat
    10261 ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg ttattttttt
    10321 tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca cacataaatc
    10381 aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata attaattggc
    10441 tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata tttatcttta
    10501 tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa gataagtaaa
    10561 ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa aatcacccac
    10621 tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg actgctatat
    10681 tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac acctgcataa
    10741 gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt ggtaaaaaaa
    10801 ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat aaaacgatac
    10861 tttgctaatg cataacccgc cattgtagaa ctggctgtag ctaaaactgt actgattaca
    10921 gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat gatggcttgc
    10981 cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa aatttcttga
    11041 ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat taaggcacca
    11101 aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt catttttatc
    11161 acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca tcgtccaggc
    11221 aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata aattgactcc
    11281 tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat ttgggatatt
    11341 gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga ttggctttag
    11401 catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta tttcagcact
    11461 ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag tgttatatcc
    11521 tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag gatttgttaa
    11581 ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc cagtcggatt
    11641 gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg gaatataata
    11701 gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa cagcaaagta
    11761 aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa cattcgtgta
    11821 agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc caacaaattc
    11881 aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg gaatcaaaat
    11941 cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt ttttatgaga
    12001 cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg aagattgcat
    12061 catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg tacttatgag
    12121 caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc caatagttat
    12181 atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa acagtatttg
    12241 atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct tagtagtcaa
    12301 actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc acgcccagct
    12361 ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat tgttgtgcca
    12421 cttgtggatt ttttgcatct ttagggacat aaaaacttga tcctcctgat actgcacctt
    12481 ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg tcagatgcat
    12541 caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac attgcagatt
    12601 cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct gagtcgatca
    12661 tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg atatttcctt
    12721 cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat tcttggtccc
    12781 cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct tttgccacct
    12841 ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca gcaaattttt
    12901 ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata atcgtttcat
    12961 tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg ccatatttgt
    13021 cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc agcacacctg
    13081 ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt accaattttg
    13141 gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt aagtcaatat
    13201 ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt gtcagtttgc
    13261 cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct gcgatgtttg
    13321 ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg gtaatgttta
    13381 gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc ttttcaag
//
LOCUS       DACDOA010000068        13169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDOA010000068 DACDOA010000000
VERSION     DACDOA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.068"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            95..337
                     /locus_tag="IWI39_001892"
     CDS             95..337
                     /locus_tag="IWI39_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP9208989.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            334..804
                     /locus_tag="IWI39_001893"
     CDS             334..804
                     /locus_tag="IWI39_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9208990.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            932..2146
                     /locus_tag="IWI39_001894"
     CDS             932..2146
                     /locus_tag="IWI39_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9208991.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2856..3044)
                     /locus_tag="IWI39_001895"
     CDS             complement(2856..3044)
                     /locus_tag="IWI39_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208992.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3135..3344
                     /locus_tag="IWI39_001896"
     CDS             3135..3344
                     /locus_tag="IWI39_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP9208993.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3456..5069
                     /locus_tag="IWI39_001897"
     CDS             3456..5069
                     /locus_tag="IWI39_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP9208994.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5104..6315
                     /locus_tag="IWI39_001898"
                     /pseudo
     CDS             5104..6315
                     /locus_tag="IWI39_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6338..7264
                     /gene="glsA"
                     /locus_tag="IWI39_001899"
     CDS             6338..7264
                     /gene="glsA"
                     /locus_tag="IWI39_001899"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9208995.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7472..8932
                     /locus_tag="IWI39_001900"
     CDS             7472..8932
                     /locus_tag="IWI39_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9208996.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9153..10916
                     /locus_tag="IWI39_001901"
     CDS             9153..10916
                     /locus_tag="IWI39_001901"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9208997.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11000..11458
                     /locus_tag="IWI39_001902"
     CDS             11000..11458
                     /locus_tag="IWI39_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9208998.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11567..12250
                     /locus_tag="IWI39_001903"
     CDS             11567..12250
                     /locus_tag="IWI39_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9208999.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12397..13011
                     /locus_tag="IWI39_001904"
     CDS             12397..13011
                     /locus_tag="IWI39_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9209000.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
BASE COUNT         4582 a         2091 c         2752 g         3744 t
ORIGIN      
        1 gcttaattat ttttattgta gtgctgatat gagaatgtta taattggggt ataaaacata
       61 caaataaatt tgctgaaaaa tgaggtatca aaatatggaa aatgaattac tggaaaactt
      121 aggaagactt cacacaactg aacttggcgt tattagaatt aaaaagaact tatctttgaa
      181 tgtagaaaat gtaattgaat ggtgcaaaga aaaaattagc ctttctaatg cggaaatcac
      241 aagaaaagga aaaaactggt atataacgat tgataattgt attataacgg taaatgcgta
      301 cagttatacc gttatcactg ctcacaaggt aaaatgataa atttttctat aaaggaatta
      361 aacgcatttg cagttattgg gcaggaagta gaactgacta attatcaaaa aaagaatatt
      421 cagattagta cacagttttg gagaaagttt aacagtagtt tgaaaaaagc gtatctttca
      481 caatcaggaa attgggtaaa atatgctttt atggaaagaa gaaatggaaa actttattat
      541 ttctgttcta ttccccaaag aaccattacc ccagagggct ttctgtataa agaaataccg
      601 tcttataaat atttggttgt ggagcatatt ggtgctatgg ataaaatata tgaaacttat
      661 ggcaagattt atgaagaaat tattcctagt acatcgtata ttcctataaa agatattatc
      721 ctacactttg aaaaatacga ttatcgtttt cattggaata gtgataattc agttattgaa
      781 atttggatac ctattcaaga ttagaagcaa ttttatatcc atacacacaa agcagttagt
      841 cttaggattg actgcttttt ctatacagat ttttaatgac aggcaattat caattatggt
      901 agttgccttt tttgattgga ggaattgaag aatgaatgat aaaaacttta ttgaagaact
      961 aagacaaaag cgtgaggaat acggagtaac acaaacaagg cttgctgttg cctgtggtat
     1021 cagccgtgaa tattacaacc gcattgaaaa aggaaaacag ccattgaatg acgaattaag
     1081 agaaatcata gaaaaacaga ttgagcgttt caatccgcaa gaaccactat tcttattgat
     1141 tgattacttt cgtgtccgct ttcctactac ggacgcattg gcgattatcc gtgatgtatt
     1201 acaactgaaa tctaattaca tgctttatga agattatgga aaatacggat atgaaagcaa
     1261 atatgtactt ggcgacatca atatcatgtg ttccatgcag gagcatttag gtgttttatt
     1321 ggaactgaaa ggcaaaggct gtcggcaaat ggaatgttat ctgctggcac aggaacgctc
     1381 atggtatgac ttcatgctgg attgtatgac ggctggtggt gtgatgaaac ggcttgattt
     1441 ggctatcaat gataaagcag gaatattgga tattccaaag ttaaaggaaa aatacaaggc
     1501 tggcgaatgt atgtcctact tccgtatgca gaaagattac agcggtacag aaaaatgcgg
     1561 tagcgattta ccaaagaata caggagaaac cttatatctc ggttcaacaa gtagcgaatt
     1621 atatatgtgt gcttatcaga aaaattatga gcagtatgtc aagaatggca cagaaattga
     1681 agatacggag attaagaacc gttttgaaat acgcatgaag aacgaacgag cctattatgc
     1741 ggttgtagat ttactgacct atcgggacgc tgaacgcacc gctttttcta tcatcaatca
     1801 ttatgtccgc tttgttgaca gagaggacga caagccaaaa agtcaatgga taacaaatga
     1861 tgattgggca tggtttgtag gggaaaacag agagccgata cgcttaacca caaagccaga
     1921 gccttacact ttacaaaaag cgttgcattg gctacaaaga caagtcgcac caaccataaa
     1981 aatggtacag gcattagaca gagaaaatcg tacaacgata ctgaaagata tgattgagca
     2041 ggcagaacta aaagacaagc ataaacattt attacaatta gagaaatcaa ccatagaaga
     2101 acgtatagat accgctgttc cacaagagaa tgacggtatt ttttaatgta attgaaaaat
     2161 ttcagattta gtcagcaaag agctgttttc attccctatg tgaagtgaaa gaagaaaaat
     2221 aaatttttta ttaaatctgc aacaaaatgg ctacttgcgt acagatatgg tgcgagaaaa
     2281 ggaaatctaa actttttttg aaaataggtc attaaatcgg ggcttgctgt ccctatatga
     2341 agtgaaagaa gaaaaaagtt ttgaaaaaat gacggaaatt cgtgattagt ggacagtctt
     2401 ctgtgaaaag aggaaaaaca tcatcatttt gtattcaaac aggcattagc agttgagtgt
     2461 tgatgtgaaa agatgaaaat actttgttct caacttaaca atttaaggtt tccgttccct
     2521 atatggtgca aagaaaatat ttcattctat tgttggcagt aacggtatgg tttgggtagt
     2581 tagggtgtga aaaggaaaat attctttttt catcatcaac ttagcaaaat gacattttct
     2641 atccctatat ggtgcatgaa aagaagttaa aatttttcca tttaaactta gcaaatggac
     2701 gtttgctgta cagatagtag tgaaaaagga gaaaatcaac cgttgattgc caaaatgaac
     2761 cgttccattt gtactaataa tggaaaaagt tttggatttt ggttacgttt ctcctctttt
     2821 ccaacgcgat atataggaaa gacgattttt ttctttcaat cgttctttga caactgaata
     2881 aagctattca atacgtttga cagacagcta gccgttgaaa caaatacgcc acgacctttc
     2941 ccctgcaaga gcgagcgaaa acctatttgt gttcttgtaa aagatttgtc ctcttttatc
     3001 gccatgacct gtactgtcag ataatgatac tcccgtacag ccacagcttg agcattaaga
     3061 gcgtcgcacg caatgggtac ggctacataa gaactatgca gaggtggaac tcctgtgggc
     3121 tatgacaaaa gccgttgaat tgcttagaaa gattttacag aaagggggta tgtgttattg
     3181 ataatgctgt aaaaacaatt caaaagaaaa atggcaagtg tggcattggc aacagaggaa
     3241 agacaaagat tgtagtaaaa gagcattttt ccgaaaaggg caaaacaatg gaagaacttc
     3301 taactgatgt catgctggaa aaagcaaagc agacaatcgc ataaaatcgg tgcagttgta
     3361 agaaatagat tgaatgacaa aaagtacccg tgttatactt tacttgcaag caggtattgt
     3421 aaacacgggt tagttataaa ggaggataac tgacaatgaa acaacagatt tacaatactg
     3481 cactttatct taggttaagc cgagatgatg aattacaggg cgaaagttcc agcattacca
     3541 cacagagaag tatgttgcgt ttatatgcaa aagaacatca tttgaatgtc attgatgaat
     3601 atattgatga cggctggtcg ggaacaaatt ttgacagacc gagttttcaa agaatgattg
     3661 aggatataga ggcaggaaaa atcaactgtg ttgtaacgaa agacctttcc cgtcttggca
     3721 gaaattatat tatgacagga caatatacag aattgtattt ccctagtcat aatgtccgct
     3781 acatagcgat tgatgacggt gtagacagcg aaaaaggcga aagtgagatt gcaccattta
     3841 agaacatcat caatgaatgg gtggcaagag atacaagccg taaagtcaaa tcagcattta
     3901 agacaaagtt tgcggagggg gctcattacg gtgcttatgc tccgttagga tataagaaac
     3961 accctgacat caaaggaaaa ctgttggttg atgatgaaac aaaatggatt attgaaaaaa
     4021 tcttttccct agcctatcaa ggttacggaa gtgcaaaaat cacaaagcag ttaagagcag
     4081 aaaaagttcc gacagcgtca tggctaaatt ttacaagata cggtactttt gcacatatct
     4141 ttgagggaaa acccgaaagt aagcgttatg agtggacgat tgctcatgtc aaggcgatat
     4201 tgaaaagtga ggtttatatc ggaaacagtg ttcacaatat gcagtctacg gtatcgttca
     4261 agagcaaaaa gaaagtgcgt aagcccgaaa gtgaatggtt tcgagtagaa aacactcacg
     4321 agccgattat tgacaaggaa gtgttctatc gtgtgcagga gcagataaaa tcaagacgca
     4381 gacagacaaa ggaaaaggca acgccgattt ttgcagggct tgtcaagtgt gcggattgtg
     4441 gttggtctat gagatttgga acgaataaga caaataaaac gccttacagt tattatgctt
     4501 gcagttacta cgggcagttt ggcaaaggta attgttctat gcactacatt cgttatgatg
     4561 tgctgtatca agccgtattg gaacgcttgc agtattgggc taaggcagta cagcaggacg
     4621 aagaaaaggt attgaacaag atacagaaag ctggcaatgc agagagaata cgggaaaaga
     4681 agaaaaaggc aagtacattg aagaaagccg agaacagaca aaatgagatt gaccgtttat
     4741 ttgcgaaaat gtatgaagat agagcctgtg agaagataac agagcgaaat tttgtgatgt
     4801 tgtccagcaa gtaccaaaaa gaacagatag aactggaaca gcagataaca agcctaagag
     4861 aagaactaag taaaatggaa caggatatga taggtgctga aaagtggatt gagttaatca
     4921 aggaatattc cgtaccaaag gaactgacag caccattatt aaatgccatg atagaaaaaa
     4981 tcctcataca tgaagcaaca acgaatgagg ataacgaaag aatacaggaa attgagatat
     5041 attaccgatt tattggaaga gttgagtaat caacaagagt aatattttta actaagggaa
     5101 accggcggtg tctatacttg ggtcagtcgc acattgggaa aacgattcgg ttttgccgca
     5161 atctttttcc aatggtttga gataacagtt ggctacttga ccatgctgta tttcttaact
     5221 ggtgcattat cctatgcaac gggtattggt gctatacaaa acaataaata tctgaaattg
     5281 gcaatcctat tgattatttt ctgggctatt ttactctcac agttaaaagg aaccaaatac
     5341 acttcattga ttgctcggat cggttttgta gctggtatct tgttgccagc attagtcttg
     5401 tttggcttag ggatccatta tgtcgcttca ggcgctccac tacaaacgat actgtctatg
     5461 aaaacattga ttcctgattt ttcgaaattg cctacgctgg ttgtttttgt ttcctttatt
     5521 ttagcatata tgggcgtaga aacatctgcc agtcatgcaa atgaaatgga aaaccctaaa
     5581 aaggattatc cattagcaat gtttgtattg gttattctgg caatcatttt agatactttt
     5641 ggcggattga ccgtcgctac tacagtacct caacatggtt tgtctcttaa tacaggggtg
     5701 attcaatcgt tagaattcct attaagacac attgatccaa atcttgcatg gattgcgaaa
     5761 attcttgcga ttcttgtttg tttaggtgtc atcggagaaa ttgcttcttg ggtaacctct
     5821 ccttcaaaag cacttcacgt agcagcagaa gaaggattac ttcctgaata ttttgcaaaa
     5881 gaaaacacac acggtgttcc tgctcatttg atgatcgcaa atgggatcgt tgcaacaatc
     5941 tgggcagccg tctttacatt aagcggcggg gggaataatc tttcatttct tgcagcgatg
     6001 tcattgactg ttgtaatcta cctgatgttg tactttatgt ttttcgcggg ctatttgaag
     6061 ctggttttta aagatgacag tcttgaaaga gcctatcagg ttccaggcgg aaaagttgta
     6121 aaagtaatcg ttgcttgttt aggtcttttg acttcacttt ttgctttttg tatctcattt
     6181 gttccacctg ctagtatcgc agctggacag catgggattt atgaaacgat tctgattgtt
     6241 tcttatctta tcgtcttagt tattccattt acgatctacg catgccgtca taaattccag
     6301 catgcagaaa aataaaaaat gaaagctggt tattcaaatg gaagtaaaag aattagaaca
     6361 attgattgga aaaaatttcc actattatca aacaggacaa gtggcggatt acattccagc
     6421 attagctgaa gtggatccca aacaattagg tatggcgatt tatgatttgg atacaaaaca
     6481 actgattgag ggaggagacg ccgatgtgcg ctttgccatc gaaagcatgt caaaagttcc
     6541 cgtattgtta ttagcaatca aagacaacgg aatagaaaaa gtctttcaaa caatcaacac
     6601 ggaaccaacc ggttttgcct tcaattctat catgaatatg gaaatcaatc atcgcaaaca
     6661 tccaatgaat ccttttgtta atgcaggagc gattgcaaca acctcgttga ttgccggtaa
     6721 agatgcagat gaaaaatttg agcgaatctt gtcatttatg aaagagatct gtgatgatcc
     6781 taatatcaca ttgaatgaag aaatctatca ttctgaatca agaacaggtg atatcaatcg
     6841 ctctcttgct tactatatga aaggtaatca aatgatcgaa ggagacgtac cggagatact
     6901 cgatgcttat ttcagacaat gctccctcaa tgtcaccgct acaggcatag caaaattagc
     6961 tgctgtgctg gcaaataaag gaattgctcc ttggaatggc aaacaaatca ttactgaaga
     7021 aagtgctaca atcgtcaaat caattatgac aactgctggt ttgtatgatg aatcaggaga
     7081 gttttccgtc catgttggtg tacctgcaaa aagcggtgta ggcggcgggt tgatggcagc
     7141 tgtgccaaat cgttacggca taggtgtctt cagcccggca ctagatccat ttggtaacag
     7201 cgcagctggt atccaattgt tgaaagatgt agtcaaagaa ctagacgctg acatctttga
     7261 ataaccattc ttgaaagagg ctggggtaaa agtccaaacg gctcaaagct acctacaatg
     7321 aggctgaaat tgtgagcttg tttgactttt gtcccagttt ctttttttat tggtttttga
     7381 aagggctttt ctaaataaaa aggaaacaaa aatacgattt acagatatga ttaaataaaa
     7441 tcataagaaa aactggaggc gtgtatcagg aatgctggaa aattacatcg agcgaaatat
     7501 atttagaaaa gtgtatctgt gtgaacaatt atttgaattc caagaaatag acatcgaaca
     7561 aacagctatt tctctaaggg tgactacacc aacgatcctt cacgatctag aaagtctagc
     7621 cgagtgtcta gaatattgta taaaagaaca agtaagagag aagcataagt ataaactcgt
     7681 attcaaacat ggtattgctc tttctgagct cacacagttt ctgtacggtc aatcttattt
     7741 tttaaagttt ttaagttatt attttaatgg tatattcacg agtacagaac tggctgatct
     7801 tgaatttatt tctttgagta aagtatatac aatcaaaaaa atcgtattgg atttttttaa
     7861 agaaaatgat tatttgaaaa ataaagaaat cattatccca gaatttgact cgcggaacat
     7921 cttgctagct ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt
     7981 agaagaagct tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata
     8041 ttcggaagat gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc
     8101 atttcccctt ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca
     8161 attagtctgt caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt
     8221 ctattatatt ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt
     8281 aagaaaagat ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga
     8341 attgatttta cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa
     8401 gcaatccttt ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga
     8461 aaggatctat cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt
     8521 ggaagagtgg aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa
     8581 attcgtcaaa agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat
     8641 ttttgttgtt tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta
     8701 tgcggaagaa ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc
     8761 tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa
     8821 tggattagaa actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca
     8881 tttgtatcat attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct
     8941 taattaggag accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc
     9001 tgattcgtgc gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta
     9061 ttgacttaga gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt
     9121 aaaagaagat agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg
     9181 caatcaatag ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag
     9241 aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga
     9301 atctaggtgt agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata
     9361 aaatcgtttg ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa
     9421 atggttttga aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta
     9481 aacatgacta ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag
     9541 ctaaagcgag agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct
     9601 ccctaagtgg tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc
     9661 taatcgtatt agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata
     9721 aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa
     9781 tgggatttga ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat
     9841 ttgaagaagt aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc
     9901 gcggttatca gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc
     9961 tgtccaccgg ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg
    10021 attatttgga taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga
    10081 taccaggcgc ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg
    10141 tggggatcgc tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac
    10201 gtccagtcgt cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg
    10261 atttagcgat caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta
    10321 gtgaccgaac acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca
    10381 aatatcttgc tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac
    10441 aaacagcgca gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta
    10501 tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa
    10561 ttttagcttt aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa
    10621 aaggaatcga agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat
    10681 tagataaatt aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag
    10741 gaggatttgg cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt
    10801 ttggtgcaga gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc
    10861 atttaacacc aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca
    10921 ttatgctcac catccagata attgtttata ctagtatctg gatggttttt tcattataaa
    10981 aagcaaaggg gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga
    11041 aaaatatcct agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa
    11101 aatggcagac gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg
    11161 ttacgatctg tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt
    11221 cctgaatgga aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc
    11281 ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc
    11341 ttattggcgg aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat
    11401 agaatttaca gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta
    11461 agaaaggtct gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt
    11521 tttatatgaa gctgtatttt tatataacat tactggagga gatattgtgg atacactgga
    11581 acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg gaaaacgagc cgctacacag
    11641 ttcttttatt atggggagaa tggctttggc tgcaaaagaa ggcggagcag ttggtattcg
    11701 agcaaatacg acagaagata tcaatgaaat caagcgaatc gttgatttac cggtaatagg
    11761 gattatcaaa agagattacg atgggtcaga agtgtttatt actgcgacag aaaaagaagt
    11821 ggaagaactt ttggagacgc aagcagaaat cattgcactg gatgcaactt ctcgtaaacg
    11881 tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc
    11941 tatggcagat atttcgactt ttgaggaagc gcttcaagcg caagaagatg gttttgatct
    12001 tttgtctacg actttagcag gctatacaga atattcacga aagacgcaga cacctgattt
    12061 tgatttattg aatcaaatca tagaacaagt cgatatccca gtaattatgg aaggacatac
    12121 tgactctcct gaacaagtag agaaagcata tgaattaggt gctttttcag tcgtagttgg
    12181 atctattatc acccgtccgc aattgatcac taaaagatat gtcgaagccg caagtaaagc
    12241 aaataactaa aaaacacgca cggcacagat tcttgacaaa aaagaaaaat tgatagatag
    12301 tttaaagcag attctaagaa gatgaaaaaa gcattacttt tcattttgtc ttcaagatct
    12361 gctttttaac gaattttata gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa
    12421 ttgacagaac gtgaaaatta caagttgctg attggttcag tcatccctag accagtagca
    12481 gtagtaacga ctaaatcagc aaaagatata gtgaatatcg cacccttcag ttacttcaat
    12541 atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc agagaaaaca agatgcagta
    12601 aaagacacag caaagaatat tcttgagaca agagaagctg ttatccatat ccttgatgag
    12661 gacaacgtag aagaagtaaa taaaacagca gccaatctgc ctaatgagga aagcgaactg
    12721 tacctgactg acttgacttt aactgattca gtaatcattt cagtaccagc attgacagaa
    12781 gcaaaagttc gttttgaaac atccttatat gaacatgtcg agatacggaa taaaaataaa
    12841 gtaacagctg atttattttt gctagaagta cagaaatatc atttaaatga aacagtctat
    12901 gatgataaga caggaaggat cgatccaaga aaattgaatg ctgtaagccg tttggcagga
    12961 aatgattatg cagcaatagg agaaattttc acgattgcac gtcccgaata aaaaggagca
    13021 ctagttatgt taaaaataag ctataaacca tcgacagatt caaaagaaat gaaaaaagaa
    13081 tatgaaacag tgaatgattt tctgcaaggg cagtacttag aagttccgcc attgcaagac
    13141 cattttgtcg ttactactgt tactttaga
//
LOCUS       DACDOA010000069        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDOA010000069 DACDOA010000000
VERSION     DACDOA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.069"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWI39_001905"
     tRNA            complement(77..149)
                     /locus_tag="IWI39_001905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWI39_001906"
     CDS             530..1099
                     /locus_tag="IWI39_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9209001.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWI39_001907"
     CDS             1086..1946
                     /locus_tag="IWI39_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9209002.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWI39_001908"
     CDS             2136..2840
                     /locus_tag="IWI39_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209003.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWI39_001909"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWI39_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9209004.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWI39_001910"
     CDS             4677..5993
                     /locus_tag="IWI39_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209005.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWI39_001911"
     CDS             5994..6863
                     /locus_tag="IWI39_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209006.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWI39_001912"
     CDS             6918..7727
                     /locus_tag="IWI39_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9209007.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWI39_001913"
     CDS             complement(7830..8459)
                     /locus_tag="IWI39_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9209008.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWI39_001914"
     CDS             8629..9921
                     /locus_tag="IWI39_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209009.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWI39_001915"
     CDS             complement(9997..10659)
                     /locus_tag="IWI39_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9209010.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWI39_001916"
     CDS             10884..11168
                     /locus_tag="IWI39_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9209011.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWI39_001917"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWI39_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9209012.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACDOA010000070        13059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDOA010000070 DACDOA010000000
VERSION     DACDOA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.070"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            573..1703
                     /locus_tag="IWI39_001918"
     CDS             573..1703
                     /locus_tag="IWI39_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9209013.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1795..2550
                     /locus_tag="IWI39_001919"
     CDS             1795..2550
                     /locus_tag="IWI39_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9209014.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2608..4647
                     /locus_tag="IWI39_001920"
     CDS             2608..4647
                     /locus_tag="IWI39_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9209015.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4756..5907)
                     /locus_tag="IWI39_001921"
     CDS             complement(4756..5907)
                     /locus_tag="IWI39_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9209016.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(6061..6591)
                     /locus_tag="IWI39_001922"
     CDS             complement(6061..6591)
                     /locus_tag="IWI39_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209017.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6690..7040)
                     /locus_tag="IWI39_001923"
     CDS             complement(6690..7040)
                     /locus_tag="IWI39_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9209018.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7197..8726)
                     /gene="ahpF"
                     /locus_tag="IWI39_001924"
     CDS             complement(7197..8726)
                     /gene="ahpF"
                     /locus_tag="IWI39_001924"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9209019.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8736..9299)
                     /gene="ahpC"
                     /locus_tag="IWI39_001925"
     CDS             complement(8736..9299)
                     /gene="ahpC"
                     /locus_tag="IWI39_001925"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9209020.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9443..10105)
                     /locus_tag="IWI39_001926"
     CDS             complement(9443..10105)
                     /locus_tag="IWI39_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9209021.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10289..11935
                     /locus_tag="IWI39_001927"
     CDS             10289..11935
                     /locus_tag="IWI39_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9209022.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11926..13008
                     /gene="uxuA"
                     /locus_tag="IWI39_001928"
     CDS             11926..13008
                     /gene="uxuA"
                     /locus_tag="IWI39_001928"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9209023.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         4327 a         2402 c         2486 g         3844 t
ORIGIN      
        1 ttagcggatt gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat
       61 ataaaaaaac acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt
      121 gcaacgggat tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa
      181 atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact
      241 cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt
      301 gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt
      361 ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga
      421 tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact
      481 gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg
      541 agatgcggtg aaaaaagatc gattggtgga ttttgggtcc ttatttgact ttgtccatga
      601 tcggtctttt agaagtatac agtgccagtt cttatcgttt attgcaagcc gatgaaaata
      661 caaaaagtct attattgcgt caactaattt tcatattttt gagttggagc gtgatcttct
      721 tagctcgttc agtcaaacta cactatttac ttcaccctaa aatagcagga tacggtttag
      781 ccttatcgat tttcttttta gtattagtaa gaatagggat attcggtgtc actgtcaacg
      841 gcgcacaacg ttggatctct ctgtttggca ttcaattcca gccttctgaa ctggcaaatc
      901 tttttttgat tttttattta agctggtttt ttcgtgacgg aaatagtagc ccaaaagatc
      961 taaaaaaacc attcctgatt acagtaggta taacttttct gattttattt cagccaaaga
     1021 ttgctggagc attgatgatc ctttcgattg cgtgggtcat attttgggca gcggcggttc
     1081 catttaaaaa agggatctat ctaatcgtta ctttttctgc attgctgatt ggagcagcag
     1141 gcggggtatt atatttagga aataaaggtt ggcttccaca gatgtttaat catgcttatg
     1201 aaaggatcgc gactttaaga gattctttta tcgatagtca tggtgcgggc tatcaaatga
     1261 cccattcgtt ttatgcacta tataatggtg gcatatttgg gcggggatta ggaaatagta
     1321 tcacaaaaaa agggtatctt ccagagacag aaacagattt tattttttcg attattacag
     1381 aagagcttgg gctaatcggt gcattgtgtg tgcttttctt attgttctca ttatgcatga
     1441 gaatcttttg tttaagcagt cgctgtaaaa atcagcaagc tggattgttt cttttaggat
     1501 tcggaacttt gctattcgtt cagacgataa tgaacgtggg aagtatcgcg gggctgatgc
     1561 caatgactgg tgtgccattg ccatttgtta gctacggagg aacaagttat ctgatccttt
     1621 ctttaggaat cggaatcacg ttgaatattt cttcaaagat acaggccgaa gagttggcgc
     1681 tctatcgacc tgaaaaacaa taaagagaaa ctgagaaact ctcagtttga attatcataa
     1741 agtacaatct tttatttttc aagcgagagg ttcttttaga aagaggaatt cgcaatgaaa
     1801 gtatctcaaa aaatcatatt atctattttg ttactgctta cattagtagt tggctatttt
     1861 tcgattgagt ttatccatgg attttcgtct gcaaaacaga gttcaactgt gaaaaaggta
     1921 gagccgaaaa gtatccctac cacactaaat gtggctttga ttggttcaga tgctcgttcg
     1981 aaagacgaaa acggtcgctc ggattcactt ctggttgcac aatacaacca gaaaacacaa
     2041 caagcaaaac tgatctctat catgagagat tcatatgtcg atattccagg ttacggaatg
     2101 aataagataa atgcagctta ctcttatggg ggagttgatt tattaaatca aacattaaag
     2161 gaaaatttca aatttgaagc tccctattat gcaagtatca catttcaaga ttttatcgat
     2221 tgcgttaatg aactgtttcc tggtggagtc aagatcgatg cagaaaaatc tctagattta
     2281 gatggcgtat atataaacaa aggagagcaa gtgatggatg gtaatacttt actccagtat
     2341 gctcgatttc gggaagacaa agaaggagac ttcgggagga tcagaaggca gcagcaagtg
     2401 atcaaagcta tctctcagca gctgaaagac gtaacttcaa tattcaagct acccaaagcg
     2461 gttgggaaat tacttggtag tatccaaacg aaccttccag aaagtgtgct tctggattgt
     2521 ggtatggatt tcctcaaaga catatgttag aataaacagg tataaatagt gaaataaagg
     2581 aatgacaagc aagagaagga ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga
     2641 acaggcgctt atattgccgg cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt
     2701 tactaccagc actatcaaga aacccaagca gtagaagctg gagaaaagac agttgagcaa
     2761 tttgtccaag ctttgaacaa gggagattat aacaaagctg cagaaatgac atcaaaaaag
     2821 gcggcaaata aaagtgcatt atctgaaaaa gagatcttag ataaatacca gaatatatac
     2881 ggtgctgctg atgtcaaagg acttcagata tcgaatctaa aagtagataa aaaagacgat
     2941 tctacttaca gtttttcata taaagcaaag atgaatacct cattaggtga attgaaagat
     3001 ctttcttata aaggaacatt agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc
     3061 aacttggttt ttccagaaat ggaaggaaat gacaaagtaa gcctgaccac acaagaagca
     3121 gcaagaggga atattataga tcgaaatggt gaaccattag caacaacagg caaactaaaa
     3181 caattaggag tcgttccaag taaacttgga gatggaggcg aaaaaacagc caatatcaaa
     3241 gcgattgctt cctccttcga cttaacagaa gatgctatca atcaggcgat ttcacaaagc
     3301 tgggtacaac ccgattactt tgtcccattg aaaatcattg atggagcaac gccagaactt
     3361 ccagctggag ctaccattca agaagtagac ggcagatatt atcctttggg tgaagcagct
     3421 gctcagctga ttggttacgt gggagatatc acagcagaag atattgataa gaatccagaa
     3481 ttaagcagta atggaaaaat cggtcgctca ggtttggaga tggcttttga taaggatctt
     3541 cgtgggacaa caggtgggaa attaagcatt acagatgcag acggtgtaga gaaaaaagtt
     3601 ctgatcgaac atgaagttca aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca
     3661 caaaaaacag cttttgacag tctaggagga aaagctggat caactgttgc gacaacgcca
     3721 aaaaccggtg atcttcttgc gcttgctagc tctccaagct atgatccaaa caaaatgaca
     3781 aacgggatct cacaagaaga ttacaaagct tatgaagaaa atcctgaaca tccattcatc
     3841 agccgatttg cgacaggtta tgctcctgga tctacgttta aaatgattac agcagcaatc
     3901 ggtctcgaca acggcactat cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg
     3961 caaaaagaca gttcttgggg atcttatcaa gcaacgcgtg tcagtagtga tgtatcacaa
     4021 gtagacttaa aaactgcttt gatctattcc gataatatat atgcggcaca agaaacgttg
     4081 aaaatgggtg agaaaaaatt tcgtacaggc ttagataaat tcatttttgg tgaagacctt
     4141 gatttgccaa ttagtatgaa tccagcacaa atttctaatg aagatagctt taactcagat
     4201 atcttgctag ctgatactgg atatggacag ggcgaacttc taattactcc tatccagcaa
     4261 gcagcaatgt attctgtttt taccaacaat ggcacacttg tctatcctaa attgattgca
     4321 gataaagaga caaaagataa gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg
     4381 ccagatctga gagaagttgt gcaagatgta aatggtacag cacattctct ttctgcttta
     4441 gggattccgt tggcagcgaa aactggtaca gcggaaatca aagaaaaaca ggatgtaaag
     4501 gggcaagaga acagtttctt gtttgctttc aatcttgata accaaggtta tatgatggtc
     4561 agcatgttgg aaaataaaga agatgatgat tcagcaacca aacgagcatc ggaactattg
     4621 caatacctca accaaaatta tcaataaaaa attgcttttt aagtacagta aaaggactgt
     4681 ggcgttatcg tcacagtcct tttttcaaat aaacggtttt cacgatgtct ctttcacaac
     4741 ttctttttga tttactcaca cttcataaac tttttttgta aaatatttga gaacaagtgg
     4801 agaaataagt gtacttaata aaatcacgat aactaaaggt gaatagtact gtggttcgat
     4861 caattgactt tgctgtccaa tttgtaaaat gatcaaagcc atttctccac gcgaaatcat
     4921 accagcaccg accatcaatg aactatgttg agaaaaaccg gcaatctgag caccaaaata
     4981 acctccaagc aatttggtca aaatcgctac aagcgtcaat atagaaataa agagtagttg
     5041 ttcactaaag tcagaaaaat caacttctaa tcctacactg acaaaaaata ctggtataaa
     5101 tactgcatat cctaaagcct ctacattgtg gtatacttct tctttaacct ttgtctgact
     5161 gacagctatc ccagcaaaaa aagcaccaat gacagagcta agcccaacaa gatccgctac
     5221 gtaagacatt cctaaacaaa taactagcga tacgatgata atagaagaat ttgcgtacac
     5281 tttttctgca aatgaaagta ggaaaggcgc aatccatttg actaataaga aaataaaaac
     5341 aaaataaatt agctgttcaa tcaaaacaag cggtaaagaa gcattgctag aagcttcccc
     5401 cgttagaaaa gataggctga aacttagaac taaaacgact aaaatgtcat ctaccacaga
     5461 agcacccaaa attgttgatc cttcttttgt attcactacg tttagttctt ttaatacttc
     5521 tactgaaata ctgacagaag ttgccgcaag aataatccca aagaaaatcg cttcatttcc
     5581 cgaaacttgg aatgctttgc ctgtcaacca gccaaaaaca accgggaaga tgatcccaag
     5641 caaagcaacg aacataccag gtcggaaata ttttttcaaa agagataaat cactttccag
     5701 accagctaaa aacattaata agatcacacc aatctccgaa aagtcatgga ctagaatatc
     5761 aggtttgacc caattcaatc cagcttggcc tagcaaaaca ccaacaagta attgtccaat
     5821 cactgctggg atacctaatc tccgcgagat atgtgagcca atcgttgttg aaatcaatat
     5881 caggcataaa ataccaataa attccattct catcattcct ttgtataaaa tatagcccga
     5941 acttagcata acatacatta ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa
     6001 tccaaaagat ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga
     6061 ttaacgtata gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa
     6121 aatcaaaata aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa
     6181 taagacgaat ccgccaatca acaacagaat actactataa acactccaac ttttagcttt
     6241 tgtattcatg ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag
     6301 aatcggcagg atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag
     6361 gaaaaaaaga ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta
     6421 agcctcaaaa agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat
     6481 aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc
     6541 agctcttaat aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa
     6601 cctcctttga cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg
     6661 agcgaaggag aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg
     6721 ccttttgcta gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc
     6781 tcgatgtctt ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg
     6841 gggacagggg cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct
     6901 agttctgttt gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata
     6961 cgatcttttc tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta
     7021 aaaatacgat tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta
     7081 tagtatataa gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac
     7141 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
     7201 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
     7261 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
     7321 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
     7381 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
     7441 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
     7501 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
     7561 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
     7621 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
     7681 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
     7741 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
     7801 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
     7861 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
     7921 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
     7981 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
     8041 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
     8101 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     8161 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     8221 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     8281 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     8341 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     8401 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     8461 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     8521 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     8581 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     8641 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     8701 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     8761 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     8821 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     8881 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     8941 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     9001 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     9061 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     9121 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     9181 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     9241 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     9301 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     9361 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     9421 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     9481 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     9541 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     9601 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     9661 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     9721 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     9781 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     9841 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     9901 ttgaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     9961 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
    10021 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
    10081 cttgagattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
    10141 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
    10201 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
    10261 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
    10321 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
    10381 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
    10441 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
    10501 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
    10561 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
    10621 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
    10681 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
    10741 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
    10801 gccatcacat acaatgagta ttcttgctgc gctactttat aaacgttttt tatctggagc
    10861 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
    10921 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
    10981 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
    11041 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
    11101 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
    11161 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggagt
    11221 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
    11281 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
    11341 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
    11401 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
    11461 tactgtgcgc ttttctaatc caaatattcc tgatatgcct cagcgaatcg ccacggatac
    11521 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
    11581 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
    11641 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
    11701 ctttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
    11761 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
    11821 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccga atgccgtacg
    11881 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
    11941 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    12001 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
    12061 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    12121 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    12181 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    12241 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    12301 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    12361 atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg cttagctcat
    12421 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    12481 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    12541 catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa
    12601 gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac tctttgtaca
    12661 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgtcaa
    12721 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    12781 tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    12841 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
    12901 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    12961 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    13021 taattttatt atgtaaataa gaaggatgtt caaattctt
//
LOCUS       DACDOA010000071        13004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDOA010000071 DACDOA010000000
VERSION     DACDOA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.071"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            148..720
                     /locus_tag="IWI39_001929"
     CDS             148..720
                     /locus_tag="IWI39_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9209024.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            952..1569
                     /locus_tag="IWI39_001930"
     CDS             952..1569
                     /locus_tag="IWI39_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9209025.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            1797..2135
                     /locus_tag="IWI39_001931"
     CDS             1797..2135
                     /locus_tag="IWI39_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209026.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2120..2974
                     /locus_tag="IWI39_001932"
     CDS             2120..2974
                     /locus_tag="IWI39_001932"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9209027.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3121..3354
                     /locus_tag="IWI39_001933"
     CDS             3121..3354
                     /locus_tag="IWI39_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP9209028.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3552..5456
                     /locus_tag="IWI39_001934"
     CDS             3552..5456
                     /locus_tag="IWI39_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9209029.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5766..6128
                     /locus_tag="IWI39_001935"
     CDS             5766..6128
                     /locus_tag="IWI39_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209030.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6145..6756
                     /locus_tag="IWI39_001936"
     CDS             6145..6756
                     /locus_tag="IWI39_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP9209031.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6858..7766)
                     /locus_tag="IWI39_001937"
     CDS             complement(6858..7766)
                     /locus_tag="IWI39_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9209032.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7808..8122)
                     /locus_tag="IWI39_001938"
     CDS             complement(7808..8122)
                     /locus_tag="IWI39_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9209033.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8421..10352
                     /locus_tag="IWI39_001939"
     CDS             8421..10352
                     /locus_tag="IWI39_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9209034.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10645..11196
                     /locus_tag="IWI39_001940"
     CDS             10645..11196
                     /locus_tag="IWI39_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209035.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11509..12051
                     /locus_tag="IWI39_001941"
     CDS             11509..12051
                     /locus_tag="IWI39_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209036.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            12562..13004
                     /locus_tag="IWI39_001942"
                     /pseudo
     CDS             12562..13004
                     /locus_tag="IWI39_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4252 a         2182 c         2710 g         3860 t
ORIGIN      
        1 agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt tttaacggaa
       61 atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc tattttaaac
      121 aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca agaaagaaca
      181 ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca tacgaaaatt
      241 aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca tgtgaaactg
      301 caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga aaaatttgaa
      361 gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct aattacttct
      421 gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct ttcggaagat
      481 gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga ggacttcaga
      541 agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg tgacgatgct
      601 ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg gatgctgcaa
      661 gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga tgaagattaa
      721 ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt ccattttctt
      781 gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg aatataaaca
      841 aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat tggcgaccac
      901 gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc gatgaattac
      961 attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt tcgttcacaa
     1021 gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta tcgattggca
     1081 gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac cggagaatgg
     1141 accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg agataccgtt
     1201 gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc agctatttct
     1261 ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca aacgacacaa
     1321 gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa tgtggaacct
     1381 gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat tgcttcctat
     1441 ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga cgaaggattg
     1501 attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt attgctttac
     1561 agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct tcattaacaa
     1621 cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt taagacagaa
     1681 cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag tttttttgtt
     1741 ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg gtgaatgtgc
     1801 attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc tttgtcgtcc
     1861 ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt gaggcagata
     1921 ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc ggacacggaa
     1981 aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact gtttggtttc
     2041 catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg cgaaaaattg
     2101 aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc atgtggtgga
     2161 gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca tattagcggc
     2221 tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata ccattatcga
     2281 tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt atgtgttatt
     2341 tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga atggcggcat
     2401 tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg caaagaaaaa
     2461 aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt tagcccagtc
     2521 cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg ttactcgtca
     2581 attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata tgaatggtac
     2641 ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct ttgtcctcat
     2701 cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt tgagttacgt
     2761 tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca gtttatggat
     2821 tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg gcgcgattgt
     2881 agtatggttt atacgcagac aagattatcc accaatttct tattattctg atggcaataa
     2941 ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag aagtgcaaaa
     3001 cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg taaagaggaa
     3061 aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga gataggaatt
     3121 atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat gcaaaaaatt
     3181 gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt attattcaac
     3241 tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga gattcaaaaa
     3301 gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt ttaacagatt
     3361 aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt ttttgttgca
     3421 atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga tgtcagtcaa
     3481 ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa ttaaatttgg
     3541 agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc gtcatcagtg
     3601 gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta ggactttcag
     3661 agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt caagcgtttc
     3721 aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca gcgattggtg
     3781 cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc ttattcggac
     3841 actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa ttgactgaaa
     3901 tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa gtagaagtgg
     3961 atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt ccagtagatg
     4021 gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt gaagctattc
     4081 cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa aacggaacga
     4141 ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt attgaactcg
     4201 tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc ttttctaaat
     4261 actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt caaaatattg
     4321 agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc ggagtgcctg
     4381 tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta aaagggagtg
     4441 aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca ggtactttaa
     4501 ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct gctgaaacgc
     4561 ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa gcggtcttaa
     4621 atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg aaaggcggcg
     4681 gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc ttgatggaaa
     4741 aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt gaacaacaag
     4801 ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg ggcattcgcg
     4861 atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg ggcgtgaaaa
     4921 atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc ggcgaacttg
     4981 gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat atcggaaaac
     5041 ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat agtccgtcct
     5101 tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg attgaaacat
     5161 ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta ggattagtaa
     5221 aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta gtgttggtct
     5281 tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg ttggttcatg
     5341 aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat aagttgaaag
     5401 gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata tcttaagaat
     5461 aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg aaactcagat
     5521 ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga gtatagagta
     5581 tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat aaaatttaaa
     5641 ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat taaggtagat
     5701 tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa tgtgaggtga
     5761 gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta aatgtaataa
     5821 aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa tgtttcagcg
     5881 attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg tgtgtctgtg
     5941 atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta cgttttttaa
     6001 aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat tctttagcga
     6061 atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat ctatctatga
     6121 aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat cgctgtttat
     6181 atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca aagccacaca
     6241 aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat tttggtagcc
     6301 gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat cattggtctt
     6361 ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga agaggaggaa
     6421 gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt attctggaca
     6481 gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat tccatacttt
     6541 acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat ctctgttgcg
     6601 gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga aacgttagaa
     6661 aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt tattatgata
     6721 gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg aaagataaaa
     6781 agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa tgaagtattt
     6841 tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc ttctttgctt
     6901 gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac ttctgcaatt
     6961 tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac cgtgatgata
     7021 agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc ttttacagca
     7081 ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg cgaaccattc
     7141 aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat ataaagtgca
     7201 atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat tagaatactc
     7261 acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt caacatgctc
     7321 tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa tgcaatagct
     7381 acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat caaacgtggc
     7441 acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt tatcagcgcg
     7501 cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt tttctctgaa
     7561 taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc atgaactgcg
     7621 tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct tatagagaaa
     7681 ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg attcgagtga
     7741 ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg ctacatacta
     7801 attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga atactaaaaa
     7861 catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat ttcactaagc
     7921 gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg tccagagaaa
     7981 tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc gacaatcgtg
     8041 taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct ttatcgattg
     8101 gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt ccgttcatat
     8161 ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag aagtggcgtt
     8221 ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac atatgattaa
     8281 ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga aaggagtcag
     8341 aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg aattataaat
     8401 tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa gaaaagcata
     8461 atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc gttttgtact
     8521 ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat cagttggtac
     8581 aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt cttcttgaag
     8641 gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct aattctcata
     8701 ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg gaaggaacat
     8761 tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa ggaagaagca
     8821 aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta atcctgcctg
     8881 atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa cttcaagtgt
     8941 tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca tccattgatg
     9001 agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat ggcgtcttcg
     9061 ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa aacaaagata
     9121 cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa acaaaagctg
     9181 cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt gccattcctc
     9241 tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt gtctatagag
     9301 gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact gtctcggcaa
     9361 cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt agttcttacc
     9421 tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc ttgaccaaag
     9481 gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa aacatgattg
     9541 atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct attctaagaa
     9601 agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt aaaggattga
     9661 cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt gatgatgttt
     9721 cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca gtcgtatacg
     9781 tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg agtgaatatg
     9841 ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta attactggtg
     9901 actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa gtgattgcga
     9961 atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa tacggcgtga
    10021 ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca gatgtgggaa
    10081 tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt ttaatgcaaa
    10141 acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac cgtgttattt
    10201 ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt agtttcttag
    10261 gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg gttgttattt
    10321 tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt caaattaatc
    10381 gagtaataga gataattaag gattacacta cattttttat ctctattact tgtttatatt
    10441 tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg cgggttgaat
    10501 ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta tagggttctt
    10561 tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc atgtattcga
    10621 acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg gtcgcaacag
    10681 gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg tgacgttttc
    10741 ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa actcaagaaa
    10801 aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg gaaaacacgc
    10861 attttatact taagcagctt acaggacttg attcacggct ataccgcacc cttagatccg
    10921 gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac ggtctaccag
    10981 atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca caccctacga
    11041 aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt aatggaaatc
    11101 tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga agatgaaatc
    11161 agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat agataagcac
    11221 agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt ggtatattat
    11281 atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca taagggtccg
    11341 gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg gtccggcggg
    11401 tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc tttttttatt
    11461 gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat gtctataaaa
    11521 gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca tctcttacaa
    11581 gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt agcgattatt
    11641 ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg cacacacttg
    11701 ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat caaaacggat
    11761 gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt ggggaagcat
    11821 atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc tattcgaaca
    11881 aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat ccctatctct
    11941 ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg catttttagc
    12001 gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta atcattaata
    12061 gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa taaagcattt
    12121 taagacgatt tcaaatcatt catgtgggtt atttatcacg gatgagcttg aaatcgtctt
    12181 ttatacgtat caaattgaaa tgttcggcat acaacctatc gttgttacta cattactaat
    12241 tgtcacttgt taaggaactt ggatcctttc taaacattcc ctagagataa attttacttt
    12301 atccctccgt taatatccaa tttaagcacc tttccgattc agggttaatt tgcatatcaa
    12361 gtgcagcttg tgttctagta agattagccc ttttagtccg gctgagttga cttatggata
    12421 taagatatgg tcatagcaaa tagatactac gtatagtctg ttaatttagt gtcattaatc
    12481 ttaagttgga taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa
    12541 aacataccag gaggaatttt tatgtctaag agaacacgaa gaactttttc acaagaattc
    12601 aagcaacaaa tcgtcaatct ttacttagct ggaaagccac gtgtagaaat cattcgagaa
    12661 tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac gagtggttca
    12721 ttcaaagaaa aagataatcc tacgcctgaa caaaaagaat tgttagaact acgtaaaaga
    12781 aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat attcggacga
    12841 agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg tgcagaatat
    12901 taggtctatc acgtcagtcc tattattatc aatcaaaacc aaagaaagac gaatcagaac
    12961 ttgaagaagc agtcgctgaa gaatttatcc gcagccgaaa ggcc
//
LOCUS       DACDOA010000072        12915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDOA010000072 DACDOA010000000
VERSION     DACDOA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.072"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="IWI39_001943"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="IWI39_001943"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9209037.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="IWI39_001944"
     CDS             complement(1036..1956)
                     /locus_tag="IWI39_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9209038.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="IWI39_001945"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="IWI39_001945"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9209039.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="IWI39_001946"
     CDS             3685..4218
                     /locus_tag="IWI39_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9209040.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="IWI39_001947"
     CDS             complement(4708..5232)
                     /locus_tag="IWI39_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209041.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="IWI39_001948"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="IWI39_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9209042.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="IWI39_001949"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="IWI39_001949"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9209043.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="IWI39_001950"
     CDS             complement(6954..7634)
                     /locus_tag="IWI39_001950"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9209044.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="IWI39_001951"
     CDS             7760..8629
                     /locus_tag="IWI39_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9209045.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="IWI39_001952"
     CDS             8655..9170
                     /locus_tag="IWI39_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209046.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9780)
                     /locus_tag="IWI39_001953"
     CDS             complement(9217..9780)
                     /locus_tag="IWI39_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209047.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="IWI39_001954"
     CDS             complement(9835..10014)
                     /locus_tag="IWI39_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209048.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="IWI39_001955"
     CDS             complement(10091..10600)
                     /locus_tag="IWI39_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209049.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="IWI39_001956"
     CDS             complement(10696..11076)
                     /locus_tag="IWI39_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9209050.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="IWI39_001957"
     CDS             11406..12155
                     /locus_tag="IWI39_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9209051.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..>12915)
                     /locus_tag="IWI39_001958"
     CDS             complement(12476..>12915)
                     /locus_tag="IWI39_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9209052.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3733 a         2612 c         2321 g         4249 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttga
//
LOCUS       DACDOA010000073        12764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDOA010000073 DACDOA010000000
VERSION     DACDOA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.073"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            20..2185
                     /gene="acm"
                     /locus_tag="IWI39_001959"
     CDS             20..2185
                     /gene="acm"
                     /locus_tag="IWI39_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9209053.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2457..2942)
                     /locus_tag="IWI39_001960"
     CDS             complement(2457..2942)
                     /locus_tag="IWI39_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9209054.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3422..4063
                     /locus_tag="IWI39_001961"
     CDS             3422..4063
                     /locus_tag="IWI39_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9209055.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4082..4717
                     /locus_tag="IWI39_001962"
     CDS             4082..4717
                     /locus_tag="IWI39_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9209056.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4735..5550
                     /locus_tag="IWI39_001963"
     CDS             4735..5550
                     /locus_tag="IWI39_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9209057.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5869..7305
                     /locus_tag="IWI39_001964"
     CDS             5869..7305
                     /locus_tag="IWI39_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9209058.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7494..9941
                     /locus_tag="IWI39_001965"
     CDS             7494..9941
                     /locus_tag="IWI39_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9209059.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10171..11238
                     /gene="rlmN"
                     /locus_tag="IWI39_001966"
     CDS             10171..11238
                     /gene="rlmN"
                     /locus_tag="IWI39_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9209060.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11431..12534)
                     /locus_tag="IWI39_001967"
     CDS             complement(11431..12534)
                     /locus_tag="IWI39_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9209061.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4359 a         2154 c         2715 g         3536 t
ORIGIN      
        1 aataggggag atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat
       61 tattactaat ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat
      121 cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat
      181 aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg
      241 gatcaattgg ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt
      301 aatgattgaa aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt
      361 gtttaatgaa aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca
      421 ggccagaaac gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg
      481 tgataaaaca gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt
      541 ttattataaa acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa
      601 tattaacaat aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag
      661 tggacaaaga ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa
      721 ggtttataga ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt
      781 taatttttca gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct
      841 taggaaaata atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga
      901 gaatcattcg gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt
      961 caatcataca gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga
     1021 attgaagatt ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga
     1081 attaagacga gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa
     1141 tgaacaggga gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa
     1201 agaagcaccc aattggattg attttgatcc actaagttca aatgaactca aattctccat
     1261 aaacgaaaat gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa
     1321 catcacagca actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa
     1381 attattcaga gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt
     1441 agacaatgga ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa
     1501 cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg
     1561 ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt
     1621 ctcaggacaa aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat
     1681 cacagtaaat ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga
     1741 tgattggcgc tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta
     1801 tactgtaacg gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa
     1861 aaacagttat acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg
     1921 gaaaaatttt ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt
     1981 gcaaatattt gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag
     2041 agctgtacct aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa
     2101 aaaatctagt tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt
     2161 cttacaaaaa tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa
     2221 atcaaaagaa taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct
     2281 tttttcgtag aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt
     2341 ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt
     2401 tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac
     2461 aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa
     2521 tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca
     2581 atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga
     2641 gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat
     2701 aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc
     2761 aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc
     2821 ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt
     2881 tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc
     2941 atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa
     3001 ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt
     3061 taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca
     3121 tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc
     3181 ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt
     3241 ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga
     3301 acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg
     3361 tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa
     3421 gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa
     3481 attattttta tttaccttga ttggctcatt accattaggg attgttttag cattaggatt
     3541 gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg
     3601 aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt
     3661 ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc
     3721 agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt
     3781 tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat
     3841 tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta
     3901 tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac
     3961 tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat
     4021 cgtaatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa
     4081 tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt
     4141 gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa
     4201 aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt
     4261 agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt
     4321 attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc
     4381 aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc
     4441 ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa
     4501 acagcgggta gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga
     4561 accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt
     4621 gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt
     4681 cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa
     4741 aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac
     4801 aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt
     4861 ttagacgata catttgttcc tatgggattt agagataaag acgggaagct cacaggattt
     4921 gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc
     4981 attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac
     5041 ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa
     5101 aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat
     5161 aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca
     5221 gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa
     5281 gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca
     5341 gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat
     5401 gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc
     5461 aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg
     5521 tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc
     5581 acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga
     5641 atggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc
     5701 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact
     5761 aagcgacttt ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg
     5821 ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa
     5881 gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta
     5941 gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata
     6001 cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat
     6061 aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct
     6121 tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat
     6181 gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat
     6241 tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt
     6301 gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac
     6361 tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat
     6421 ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca
     6481 aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc
     6541 tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa
     6601 aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg
     6661 attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat
     6721 gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg
     6781 acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa
     6841 aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt
     6901 gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt
     6961 cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat
     7021 gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc
     7081 cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc
     7141 tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta
     7201 tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat
     7261 tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa
     7321 caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag
     7381 aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt
     7441 agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac
     7501 aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc
     7561 agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg
     7621 gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag
     7681 gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta
     7741 agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt
     7801 tcggtatctt attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa
     7861 atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca
     7921 ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg
     7981 cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca
     8041 gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg
     8101 aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa
     8161 tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa
     8221 aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg
     8281 atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca
     8341 gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac
     8401 agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag
     8461 aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc
     8521 ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg
     8581 gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct
     8641 taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa
     8701 ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag
     8761 caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg
     8821 tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac
     8881 ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact
     8941 tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa
     9001 gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg
     9061 ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta
     9121 gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat
     9181 tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact
     9241 tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag
     9301 ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa
     9361 tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta
     9421 tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta
     9481 cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata
     9541 tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg
     9601 accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag
     9661 agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg
     9721 agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc
     9781 tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag
     9841 ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg
     9901 aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt
     9961 tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt
    10021 ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag
    10081 ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag
    10141 cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg
    10201 aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa
    10261 gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg
    10321 aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc
    10381 gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg
    10441 attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta
    10501 ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg
    10561 actgctggag aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat
    10621 cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac
    10681 gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct
    10741 cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac
    10801 ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg
    10861 atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat
    10921 ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat
    10981 agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat
    11041 gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa
    11101 gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt
    11161 aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa
    11221 aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt
    11281 tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat
    11341 aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac
    11401 agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct
    11461 ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt
    11521 gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc
    11581 taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt
    11641 cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag
    11701 tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt
    11761 accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac
    11821 caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc
    11881 gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt
    11941 tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt
    12001 agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc
    12061 ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag
    12121 tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt
    12181 cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac
    12241 agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc
    12301 atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc
    12361 gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc
    12421 agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt
    12481 ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat
    12541 ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc
    12601 tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca
    12661 ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca
    12721 ataatgaatt tttgttcctc ttagtatttc taccgattcc cttt
//
LOCUS       DACDOA010000074        12739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDOA010000074 DACDOA010000000
VERSION     DACDOA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.074"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IWI39_001968"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IWI39_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9209062.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IWI39_001969"
     CDS             complement(323..892)
                     /locus_tag="IWI39_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9209063.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IWI39_001970"
     CDS             1078..1980
                     /locus_tag="IWI39_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9209064.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IWI39_001971"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IWI39_001971"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9209065.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IWI39_001972"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IWI39_001972"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9209066.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IWI39_001973"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IWI39_001973"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9209067.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IWI39_001974"
     CDS             8548..8733
                     /locus_tag="IWI39_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9209068.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IWI39_001975"
     CDS             complement(8795..9991)
                     /locus_tag="IWI39_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9209069.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IWI39_001976"
     CDS             complement(10161..11597)
                     /locus_tag="IWI39_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9209070.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="IWI39_001977"
     CDS             11890..12699
                     /locus_tag="IWI39_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9209071.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4013 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaattt
//
LOCUS       DACDOA010000075        12701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDOA010000075 DACDOA010000000
VERSION     DACDOA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.075"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(116..478)
                     /locus_tag="IWI39_001978"
     CDS             complement(116..478)
                     /locus_tag="IWI39_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209072.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(552..932)
                     /locus_tag="IWI39_001979"
     CDS             complement(552..932)
                     /locus_tag="IWI39_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9209073.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1014..2189
                     /locus_tag="IWI39_001980"
     CDS             1014..2189
                     /locus_tag="IWI39_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9209074.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2266..2850)
                     /locus_tag="IWI39_001981"
     CDS             complement(2266..2850)
                     /locus_tag="IWI39_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9209075.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3051..4049
                     /locus_tag="IWI39_001982"
     CDS             3051..4049
                     /locus_tag="IWI39_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9209076.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4161..4670
                     /locus_tag="IWI39_001983"
     CDS             4161..4670
                     /locus_tag="IWI39_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9209077.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4700..5359)
                     /locus_tag="IWI39_001984"
     CDS             complement(4700..5359)
                     /locus_tag="IWI39_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9209078.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5361..6125)
                     /locus_tag="IWI39_001985"
     CDS             complement(5361..6125)
                     /locus_tag="IWI39_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9209079.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6133..6810)
                     /locus_tag="IWI39_001986"
     CDS             complement(6133..6810)
                     /locus_tag="IWI39_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9209080.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6847..8232)
                     /locus_tag="IWI39_001987"
     CDS             complement(6847..8232)
                     /locus_tag="IWI39_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9209081.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8442..8526)
                     /gene="ffs"
                     /locus_tag="IWI39_001988"
     ncRNA           complement(8442..8526)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWI39_001988"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8549..9064)
                     /gene="tadA"
                     /locus_tag="IWI39_001989"
     CDS             complement(8549..9064)
                     /gene="tadA"
                     /locus_tag="IWI39_001989"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9209082.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9171..10028
                     /locus_tag="IWI39_001990"
     CDS             9171..10028
                     /locus_tag="IWI39_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9209083.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10109..10444)
                     /locus_tag="IWI39_001991"
     CDS             complement(10109..10444)
                     /locus_tag="IWI39_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209084.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10511..11881)
                     /locus_tag="IWI39_001992"
     CDS             complement(10511..11881)
                     /locus_tag="IWI39_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9209085.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11959..12495)
                     /locus_tag="IWI39_001993"
     CDS             complement(11959..12495)
                     /locus_tag="IWI39_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9209086.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3808 a         2575 c         2112 g         4206 t
ORIGIN      
        1 taaaatgcac taaaaaacta tctttagcag aacatttcat tgtatttttt ggaccaaaca
       61 ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct gtattttaga
      121 agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga cctggttctt
      181 cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt tcaaagacaa
      241 acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa ctaatcttgc
      301 taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa ccagtctttc
      361 cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg accaaaaatt
      421 ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg cttgccaaaa
      481 tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca ttttcagaaa
      541 aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc ttcttctatc
      601 caaataggta actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa
      661 tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa
      721 ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact
      781 tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt
      841 ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt
      901 ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt
      961 atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg
     1021 aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg
     1081 agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc
     1141 ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca
     1201 ctctgtctga taaactatat caggctatca ttcattggga acgccaacac catgctgtgg
     1261 agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag
     1321 tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat
     1381 tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa
     1441 ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa
     1501 aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat
     1561 tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc
     1621 atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt
     1681 tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca
     1741 aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag
     1801 tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg
     1861 agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag
     1921 ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt
     1981 acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc
     2041 ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg
     2101 ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa
     2161 tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct
     2221 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2281 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2341 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2401 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2461 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2521 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     2581 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     2641 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     2701 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     2761 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     2821 actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca
     2881 taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt
     2941 tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga
     3001 gaagcattct caattaaatt ctgatatagt gtgaaaaggg aggaattttt atggaaatct
     3061 atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa
     3121 tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa
     3181 ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg
     3241 aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag
     3301 aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca
     3361 tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat
     3421 tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt
     3481 tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt
     3541 ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat
     3601 ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc
     3661 cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa
     3721 aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat
     3781 tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta
     3841 ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta
     3901 cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga
     3961 acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct
     4021 tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa
     4081 cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa
     4141 aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag
     4201 aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac
     4261 aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc
     4321 taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag
     4381 aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg
     4441 gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga
     4501 attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg
     4561 gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca
     4621 gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa
     4681 aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt
     4741 ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc
     4801 tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata
     4861 accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta
     4921 tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca
     4981 accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag
     5041 tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg
     5101 ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga
     5161 gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat
     5221 ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac
     5281 caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact
     5341 gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta
     5401 gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa
     5461 gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg
     5521 attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct
     5581 ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac
     5641 cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg
     5701 aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa
     5761 ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct
     5821 aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct
     5881 gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga
     5941 atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg
     6001 gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc
     6061 ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag
     6121 tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac
     6181 ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg
     6241 atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata
     6301 cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc
     6361 gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc
     6421 caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt
     6481 taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc
     6541 actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg
     6601 ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt
     6661 gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt
     6721 gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac
     6781 gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc
     6841 ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa
     6901 ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa
     6961 catcaaatga tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt
     7021 ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa
     7081 aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt
     7141 ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg
     7201 catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc
     7261 agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc
     7321 ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg
     7381 caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat
     7441 ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg
     7501 cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta
     7561 ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg
     7621 ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc
     7681 ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac
     7741 taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag
     7801 cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat
     7861 tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag
     7921 gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac
     7981 gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt
     8041 ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc
     8101 tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa
     8161 ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac
     8221 tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt
     8281 cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt
     8341 acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat
     8401 aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc
     8461 ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc
     8521 ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta
     8581 atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt
     8641 cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat
     8701 tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta
     8761 cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg
     8821 cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt
     8881 ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt
     8941 ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg
     9001 cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta
     9061 ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc
     9121 tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg
     9181 ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg
     9241 atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc
     9301 aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc
     9361 catggtatca ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga
     9421 tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt
     9481 tggaattatt tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag
     9541 tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt
     9601 tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca
     9661 ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc
     9721 tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag
     9781 atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa
     9841 tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa
     9901 cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg
     9961 gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac
    10021 ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga
    10081 atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc
    10141 ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt
    10201 ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac
    10261 catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta
    10321 ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg
    10381 ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt
    10441 tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga
    10501 actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta
    10561 cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc
    10621 catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca
    10681 agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc
    10741 gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag
    10801 aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag
    10861 cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt
    10921 gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag
    10981 tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt
    11041 tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag
    11101 gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt
    11161 cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta
    11221 ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc
    11281 actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa
    11341 ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg
    11401 tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt
    11461 tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg
    11521 ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag
    11581 cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga
    11641 ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta
    11701 attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc
    11761 gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc
    11821 ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca
    11881 ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct
    11941 actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc
    12001 cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc
    12061 atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt
    12121 gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc
    12181 taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa
    12241 taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata
    12301 aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata
    12361 aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat
    12421 tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt
    12481 tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata aataacataa
    12541 ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat
    12601 caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata
    12661 gaataaacaa ggtaacaaaa aaattaaata acgttatata a
//
LOCUS       DACDOA010000076        12432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDOA010000076 DACDOA010000000
VERSION     DACDOA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.076"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..190)
                     /locus_tag="IWI39_001994"
     CDS             complement(<1..190)
                     /locus_tag="IWI39_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209087.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="IWI39_001995"
     CDS             complement(200..1015)
                     /locus_tag="IWI39_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9209088.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="IWI39_001996"
     CDS             complement(1277..1720)
                     /locus_tag="IWI39_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9209089.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="IWI39_001997"
     CDS             complement(1854..2192)
                     /locus_tag="IWI39_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9209090.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="IWI39_001998"
     CDS             complement(2180..2557)
                     /locus_tag="IWI39_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9209091.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="IWI39_001999"
     CDS             2781..3974
                     /locus_tag="IWI39_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9209092.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="IWI39_002000"
     CDS             complement(4140..4562)
                     /locus_tag="IWI39_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9209093.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="IWI39_002001"
     CDS             4957..5151
                     /locus_tag="IWI39_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209094.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="IWI39_002002"
     CDS             5148..5594
                     /locus_tag="IWI39_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209095.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="IWI39_002003"
     CDS             complement(5804..6976)
                     /locus_tag="IWI39_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9209096.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="IWI39_002004"
     CDS             complement(7182..7997)
                     /locus_tag="IWI39_002004"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9209097.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="IWI39_002005"
     CDS             complement(8147..8593)
                     /locus_tag="IWI39_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9209098.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="IWI39_002006"
     CDS             complement(8590..9594)
                     /locus_tag="IWI39_002006"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9209099.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="IWI39_002007"
     CDS             complement(9634..9963)
                     /locus_tag="IWI39_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9209100.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="IWI39_002008"
     CDS             complement(10089..12416)
                     /locus_tag="IWI39_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9209101.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DACDOA010000077        12245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDOA010000077 DACDOA010000000
VERSION     DACDOA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.077"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..681)
                     /gene="frr"
                     /locus_tag="IWI39_002009"
     CDS             complement(124..681)
                     /gene="frr"
                     /locus_tag="IWI39_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9209102.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(684..1406)
                     /locus_tag="IWI39_002010"
     CDS             complement(684..1406)
                     /locus_tag="IWI39_002010"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9209103.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1542..2423)
                     /locus_tag="IWI39_002011"
     CDS             complement(1542..2423)
                     /locus_tag="IWI39_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9209104.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2522..3304)
                     /gene="rpsB"
                     /locus_tag="IWI39_002012"
     CDS             complement(2522..3304)
                     /gene="rpsB"
                     /locus_tag="IWI39_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9209105.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3663..4142
                     /locus_tag="IWI39_002013"
     CDS             3663..4142
                     /locus_tag="IWI39_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9209106.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4359..5450
                     /gene="phnW"
                     /locus_tag="IWI39_002014"
     CDS             4359..5450
                     /gene="phnW"
                     /locus_tag="IWI39_002014"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9209107.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5443..5571
                     /locus_tag="IWI39_002015"
     CDS             5443..5571
                     /locus_tag="IWI39_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9209108.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5575..6120
                     /locus_tag="IWI39_002016"
     CDS             5575..6120
                     /locus_tag="IWI39_002016"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9209109.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6574..8265)
                     /locus_tag="IWI39_002017"
     CDS             complement(6574..8265)
                     /locus_tag="IWI39_002017"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9209110.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8685..9632)
                     /gene="arcC"
                     /locus_tag="IWI39_002018"
     CDS             complement(8685..9632)
                     /gene="arcC"
                     /locus_tag="IWI39_002018"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9209111.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9747..10766)
                     /gene="argF"
                     /locus_tag="IWI39_002019"
     CDS             complement(9747..10766)
                     /gene="argF"
                     /locus_tag="IWI39_002019"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9209112.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10857..12086)
                     /gene="arcA"
                     /locus_tag="IWI39_002020"
     CDS             complement(10857..12086)
                     /gene="arcA"
                     /locus_tag="IWI39_002020"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9209113.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3356 a         2531 c         2206 g         4152 t
ORIGIN      
        1 tgaaatcatt gtttttttat ttaaaaactg aataattttt tcttttttta atttttaaat
       61 aaaagaaata taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt
      121 ttattaaact tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttgat
      181 gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat
      241 atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat
      301 cgcgattttt gcattttctg cttctttttt aacatcttta gctaattctt ttctgcgttc
      361 ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc
      421 gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt
      481 gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg
      541 cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg
      601 gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc
      661 tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct
      721 aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca
      781 ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact
      841 tccagatgag tcagttcttc aaattttact gcatttgcat ctactttcgg atcagcagaa
      901 taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgcg
      961 cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata
     1021 acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt
     1081 tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc
     1141 tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt
     1201 tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccgcc cacaacgatt
     1261 gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact
     1321 ggtggtttga ttccaaatcc tgcgtcccca gctaaagctt ctccactcaa ttttaagact
     1381 acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta
     1441 ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg
     1501 gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc
     1561 ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt
     1621 acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga
     1681 tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct
     1741 tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga
     1801 acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga
     1861 ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct
     1921 agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg
     1981 tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt
     2041 gcttcgatca agtcagattc aattgtacct ttatctgttt tgattttcat tgcagcttcc
     2101 atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac
     2161 atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta
     2221 cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc
     2281 tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca
     2341 actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc
     2401 attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa
     2461 gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa
     2521 attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg
     2581 ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga
     2641 tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg
     2701 aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg
     2761 ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt
     2821 acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt
     2881 cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt
     2941 cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg
     3001 tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt
     3061 ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt
     3121 cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt
     3181 caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc
     3241 gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg
     3301 ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg
     3361 caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa
     3421 gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg
     3481 ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct
     3541 ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta
     3601 ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga
     3661 aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc
     3721 gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa
     3781 cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt
     3841 cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc
     3901 ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga
     3961 tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac
     4021 ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg
     4081 aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt
     4141 aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta
     4201 ttgaataaac ctttacactc tttacaaaaa tacatcattt tttattcaaa tttttcaaca
     4261 ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc
     4321 tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaacttctg
     4381 gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg
     4441 atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat cttgctcaag
     4501 tatcggaagg aatatacaca acggtcttaa tgcaaggtag cagctccttc ggggtagaat
     4561 cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat
     4621 atagacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg
     4681 tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccgt
     4741 ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg
     4801 aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac gcgatgtcaa
     4861 gctttggtgg gataccgatt tgtgtagccg aactagggat tgattttttg atttccagct
     4921 caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat aaagcggaac
     4981 tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac cagtaccgcg
     5041 tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt
     5101 ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt
     5161 atctcgccaa taatcatgtg ctacgacaaa aaatgacaga gatggggttc cagcctttca
     5221 ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg
     5281 attttaatca tttttatcag tatttaaagc aaaatggatt tgtgatctat cccgggaaaa
     5341 tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt
     5401 tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt
     5461 tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga
     5521 aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcta accgatggga
     5581 aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg
     5641 atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt
     5701 gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa
     5761 acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgaatgg atttaatgct
     5821 cctatgatga caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc agacttcatg
     5881 gcaacttttg aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac
     5941 atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca
     6001 gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg
     6061 ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa
     6121 aacagtctgt tgctcttaga ttccaacaag ccggcgctga tatttgtgta aacaatttaa
     6181 aagagttaac agaatacatt ttgtacgctg acatgaatcc agaatgactt ttatacaaaa
     6241 taagaggttg tgaaaaagct gtcctgtatc aagtaataaa aacagccaag caaaaatagc
     6301 tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
     6361 tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttgcaaa
     6421 caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt
     6481 atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc
     6541 ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc
     6601 tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc
     6661 ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg
     6721 tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa
     6781 gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa
     6841 tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg
     6901 agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag
     6961 gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac
     7021 cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg
     7081 ttctttagca gattggattg cttcatttaa tacttcttct aataagacga tctttccttt
     7141 acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat ggtgcatgtc
     7201 gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct
     7261 ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa
     7321 tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc
     7381 tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa
     7441 atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc
     7501 gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga
     7561 ttcatcacgg aaccattgcc ataattgaat agcctcttta tcgccttctt ccagacgttt
     7621 gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg
     7681 gacgtataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca
     7741 ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat
     7801 gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt
     7861 cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg
     7921 tactgttcct tgattcccga tgttgctatc cccataatgt tctttttctt tcactactgt
     7981 ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac
     8041 aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg
     8101 ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg
     8161 ttcagctgat tttggatttt ctagtaattg ctcgacttgt tccagtgtta aatcatcttt
     8221 gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa
     8281 agtgttccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat
     8341 cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca
     8401 tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta
     8461 tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc
     8521 tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg
     8581 aaaggctagt cgtttcataa aaaaacaatc cagatggcct aaaggagaag aaataccacc
     8641 tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc agctacgata
     8701 atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt gatgattgcc
     8761 ttagcgtttg gttttgcttc aacaaatgca atagctgctt ctactttcgg aagcatgctt
     8821 cctggagcga attgcttttc atcaatgtat tgtttcattt ctgaaacagt aacagtttct
     8881 aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc tgtcaatacg
     8941 atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc tttatcgatt
     9001 acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc accgccacca
     9061 acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc
     9121 gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc ttctttgaat
     9181 gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta gaatggaccg
     9241 actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac ttgagtgact
     9301 aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat ggcgttttgt
     9361 aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg cattgccggt
     9421 gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg cggaccattt
     9481 ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata ctttgctgtt
     9541 tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat ggcattgccc
     9601 cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc agttctaccg
     9661 aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg ctgactcgtt
     9721 ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt acctaatgtt
     9781 gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa ttgacgagca
     9841 tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa gcgttctttc
     9901 atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg caagaagatc
     9961 aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta aggtttcaac
    10021 aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac atcagaataa
    10081 aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa ttgtgcaccg
    10141 gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg taattctttt
    10201 ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa agagttagcc
    10261 atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt tccaaagttt
    10321 tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc agtcaagcca
    10381 ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg actgaatcca
    10441 cggaattcga ttccgtcaaa catacgtcct agtactttag ctgtatcttc tgtggattct
    10501 tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc tagatcgatc
    10561 gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa aagcgcaata
    10621 ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt caagtgttct
    10681 gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc taataagctt
    10741 cttccttgga atactgattc tttcatatta aacattctcc tttagattgt ttttatttaa
    10801 gattaaagag gaaatcccga cggcagctga cagtaagact gccgtcagga aacaaattac
    10861 ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg acctcgtgac
    10921 aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa ttcgttcgat
    10981 acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt gtttgatcca
    11041 tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg gatcagtaca
    11101 agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga atgagtaatc
    11161 ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc tttactttga
    11221 attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt atccaaatgc
    11281 atcattgcac ggttgttcgg aattttgatt gccaacactt tttcaaatcc tgaatttttt
    11341 gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg agaaatccca
    11401 acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg atctggattt
    11461 tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg tttcaaaata
    11521 tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt gactgtcatc
    11581 ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat tgggtccatg
    11641 taaaatggat agtcatcgtc tgctgacaga tcatatagac tgctagaacg tacttcgata
    11701 tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc cattgataga
    11761 aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc atccaacatt
    11821 ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc taatttttcc
    11881 aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc atgttcttct
    11941 tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat atctggtgtc
    12001 aaattttcca cttcttgtcc aggccgtttc agtaaaactg tctttaattt ccctatttca
    12061 gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga ttacatgtat
    12121 aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat gagacattcc
    12181 tcacattaat attcgataat taaataatta aacgtttttc tatttttaag cgtaatttat
    12241 tcatt
//
LOCUS       DACDOA010000078        12084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDOA010000078 DACDOA010000000
VERSION     DACDOA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.078"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..907
                     /gene="gnd"
                     /locus_tag="IWI39_002021"
     CDS             5..907
                     /gene="gnd"
                     /locus_tag="IWI39_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9209114.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            909..1910
                     /locus_tag="IWI39_002022"
     CDS             909..1910
                     /locus_tag="IWI39_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9209115.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1907..2191
                     /locus_tag="IWI39_002023"
     CDS             1907..2191
                     /locus_tag="IWI39_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9209116.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2224..3021
                     /locus_tag="IWI39_002024"
     CDS             2224..3021
                     /locus_tag="IWI39_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9209117.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3035..3868
                     /locus_tag="IWI39_002025"
     CDS             3035..3868
                     /locus_tag="IWI39_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209118.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3886..4743
                     /locus_tag="IWI39_002026"
     CDS             3886..4743
                     /locus_tag="IWI39_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9209119.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4765..5274
                     /locus_tag="IWI39_002027"
     CDS             4765..5274
                     /locus_tag="IWI39_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209120.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5311..5712
                     /locus_tag="IWI39_002028"
     CDS             5311..5712
                     /locus_tag="IWI39_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209121.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5824..6177
                     /locus_tag="IWI39_002029"
     CDS             5824..6177
                     /locus_tag="IWI39_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209122.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6211..6654
                     /locus_tag="IWI39_002030"
     CDS             6211..6654
                     /locus_tag="IWI39_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9209123.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6671..7756
                     /gene="uxuA"
                     /locus_tag="IWI39_002031"
     CDS             6671..7756
                     /gene="uxuA"
                     /locus_tag="IWI39_002031"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9209124.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7749..8462
                     /locus_tag="IWI39_002032"
     CDS             7749..8462
                     /locus_tag="IWI39_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9209125.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9214..9414
                     /locus_tag="IWI39_002033"
     CDS             9214..9414
                     /locus_tag="IWI39_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9209126.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            9982..10545
                     /locus_tag="IWI39_002034"
     CDS             9982..10545
                     /locus_tag="IWI39_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209127.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(10606..10971)
                     /locus_tag="IWI39_002035"
     CDS             complement(10606..10971)
                     /locus_tag="IWI39_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209128.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11047..11292)
                     /locus_tag="IWI39_002036"
     CDS             complement(11047..11292)
                     /locus_tag="IWI39_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209129.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(11362..11616)
                     /locus_tag="IWI39_002037"
     CDS             complement(11362..11616)
                     /locus_tag="IWI39_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209130.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(11780..11992)
                     /locus_tag="IWI39_002038"
     CDS             complement(11780..11992)
                     /locus_tag="IWI39_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209131.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT         4140 a         1907 c         2572 g         3465 t
ORIGIN      
        1 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
       61 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
      121 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
      181 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
      241 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
      301 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
      361 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
      421 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
      481 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
      541 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
      601 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
      661 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
      721 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
      781 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
      841 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
      901 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
      961 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     1021 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     1081 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     1141 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     1201 tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat aatataacta
     1261 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     1321 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     1381 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     1441 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     1501 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     1561 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     1621 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     1681 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     1741 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     1801 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     1861 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     1921 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     1981 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     2041 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     2101 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     2161 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     2221 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     2281 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     2341 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     2401 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     2461 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     2521 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     2581 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     2641 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct
     2701 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     2761 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     2821 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     2881 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     2941 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     3001 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     3061 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     3121 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     3181 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     3241 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     3301 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     3361 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     3421 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     3481 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     3541 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     3601 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     3661 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     3721 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     3781 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     3841 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
     3901 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
     3961 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
     4021 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
     4081 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
     4141 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
     4201 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
     4261 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
     4321 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
     4381 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
     4441 acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
     4501 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
     4561 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
     4621 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
     4681 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
     4741 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
     4801 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
     4861 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
     4921 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
     4981 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
     5041 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
     5101 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
     5161 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
     5221 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
     5281 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
     5341 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
     5401 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
     5461 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
     5521 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
     5581 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
     5641 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
     5701 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
     5761 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
     5821 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
     5881 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
     5941 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
     6001 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
     6061 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
     6121 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
     6181 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
     6241 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
     6301 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
     6361 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
     6421 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
     6481 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
     6541 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
     6601 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
     6661 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
     6721 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
     6781 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
     6841 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
     6901 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
     6961 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
     7021 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
     7081 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
     7141 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
     7201 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
     7261 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
     7321 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
     7381 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
     7441 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
     7501 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
     7561 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
     7621 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
     7681 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
     7741 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
     7801 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
     7861 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
     7921 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
     7981 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
     8041 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
     8101 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
     8161 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
     8221 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
     8281 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
     8341 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
     8401 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
     8461 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
     8521 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
     8581 aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga
     8641 gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt
     8701 gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata
     8761 gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag
     8821 ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc
     8881 atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag
     8941 ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact
     9001 ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt
     9061 gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag
     9121 cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa
     9181 cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc
     9241 aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat
     9301 ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac
     9361 gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa
     9421 ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg
     9481 gtcgttgaat gggctgaata gtagtgtata aaacaatttt gtggagcgtg aagcttttat
     9541 ggtagaggaa aatacgaata aagtgatgtt gataaaaaga ttcctatctt gaatatgaat
     9601 cgagatgtct tgaaaatgaa tgagtttgac caatcattat cctatcgaaa tttacagttt
     9661 cgttcgattg gtctgtcaat taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc
     9721 ggcacatcta aatttttaag taacggacag ttcaaatatc aagcattgaa agaggctatc
     9781 ctactgtttg aaggaatacg aatatataaa aacgacaaag aggaaaaagt agttcattaa
     9841 atcccttcaa gtgatgaaag aatgagatct aataaaacct gatctttcat ctgttttttt
     9901 aataacgtaa taattttttt attttttgtg ctttcgccgt tattgtagag tgatttagca
     9961 ttcttatttt taaaaataac tgtgttatat attaaaaaag gcataatagt tataagcttt
    10021 tttaaagaat atttagaaat tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt
    10081 attttagtct attttcaggg ggggaacctt atgataaaaa aactattttt actatcaata
    10141 ttaacgctag caatggcagg ttgttctgac tctggcttag caactgaact gatcacaaaa
    10201 tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg atcacagtga ctacagaggc
    10261 ttaaatcgac gatgcatggg aatattaggc agaagcaatt gtaagcatcg atgtagtaat
    10321 cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa agatcaaaag aagcaatctg
    10381 tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt taaaccacgt caggagatgt
    10441 tcatttatgt ctagggaagt tggcgatgaa ccaaaactag agtacgcttt tagttttgca
    10501 cgtgaggatg aagataaccc taaaacttac gacattttag cttgatctaa gatggttaaa
    10561 gctttttaat gaagtaagag gagatagaag gcttttctag ttatattagg taagataaat
    10621 tcctttttgc tccaaatttg ctaaacattc tgctagataa acttcatcgt gttccggata
    10681 aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg
    10741 atacatagga tttttccact ctggtgctgg gctgtaacca cgtttttcca tttcatccat
    10801 gacaagctga tgatattgga ataatctata aggagaatag tgaaaaacat aatccacagt
    10861 agcatgtttc tttccccagc catttcctct taatgcacag cattcccgat gttgtcctaa
    10921 aagctgttga cgaggaagct ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc
    10981 tcctttatat atagattgtt aaccttagac gttaaatgaa aaaaccagca ccaaggctgg
    11041 aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca attaggtaat caacggaaat
    11101 atcgtaaaag tcagcgatat agattaattc aatgatgctt ggaatttctt ctccttcttc
    11161 gtacttacga tatttgaata aaggaatact taacgcgcta gaaacagcta cttgagataa
    11221 agatcggcca gttcttaaga aattcagacg acaacgtaaa gtatccagta aaatagcttc
    11281 ttcgatactc atttaatcac ctttcattat agtagaatag ttataatgac tatagaaagc
    11341 tacacatgca atcaatagta ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac
    11401 gatcaataaa gacaaaacca gtcctgataa ttcttctata aaaacactat cgggttcaaa
    11461 gtagttgtaa ttaaaaactt tccacataaa gtagatagcc ggaagagtca aaatagctaa
    11521 ataaaaccgt tctttgttcc aattcataag ataaacaata cttgcgacca gtgcgaccgc
    11581 aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa cggactcctc aacgattcat
    11641 ataataaaag ataacatgtt attgcataca tcacaaatga aatgctagga aaataaaaga
    11701 agaatagatc aatgaaaaat gaaaaagcag aacagttttc tgctgataag caggaactgt
    11761 tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg attgatatct aagtaattca
    11821 atacatataa aaagaggacg atcgcattca ctaaaaagga aagaatgatg atcccatagc
    11881 gaatggaaac atatttaaca aatacttttg tttgaatacg ggcatatctt tttcctttat
    11941 agctgagcat catgacaaga agcaaaaggg taatcacacc ttgaaaaatc attccaataa
    12001 acagaattcc tgaggtttga taatttaatt ggagaaaata agctgctgcc gcaactaaat
    12061 cgaataaaat cgcaataaat ggca
//
LOCUS       DACDOA010000079        11916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDOA010000079 DACDOA010000000
VERSION     DACDOA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.079"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..576)
                     /locus_tag="IWI39_002039"
     CDS             complement(<1..576)
                     /locus_tag="IWI39_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9209132.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKV"
     gene            complement(759..1385)
                     /locus_tag="IWI39_002040"
     CDS             complement(759..1385)
                     /locus_tag="IWI39_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9209133.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1407..2738)
                     /locus_tag="IWI39_002041"
     CDS             complement(1407..2738)
                     /locus_tag="IWI39_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9209134.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2893..3294)
                     /locus_tag="IWI39_002042"
     CDS             complement(2893..3294)
                     /locus_tag="IWI39_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9209135.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3380..3586)
                     /locus_tag="IWI39_002043"
     CDS             complement(3380..3586)
                     /locus_tag="IWI39_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209136.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3681..5183)
                     /locus_tag="IWI39_002044"
     CDS             complement(3681..5183)
                     /locus_tag="IWI39_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9209137.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5201..5656)
                     /gene="argR"
                     /locus_tag="IWI39_002045"
     CDS             complement(5201..5656)
                     /gene="argR"
                     /locus_tag="IWI39_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9209138.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5754..6371)
                     /locus_tag="IWI39_002046"
     CDS             complement(5754..6371)
                     /locus_tag="IWI39_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209139.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6592..7965
                     /locus_tag="IWI39_002047"
     CDS             6592..7965
                     /locus_tag="IWI39_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9209140.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8040..9152
                     /locus_tag="IWI39_002048"
     CDS             8040..9152
                     /locus_tag="IWI39_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9209141.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9224..10861)
                     /locus_tag="IWI39_002049"
     CDS             complement(9224..10861)
                     /locus_tag="IWI39_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9209142.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11029..11637)
                     /locus_tag="IWI39_002050"
     CDS             complement(11029..11637)
                     /locus_tag="IWI39_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9209143.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3164 a         2495 c         2143 g         4114 t
ORIGIN      
        1 aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga
       61 tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt
      121 ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg
      181 tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc
      241 cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt
      301 ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc
      361 agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac
      421 cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc
      481 agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc
      541 tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc
      601 atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact
      661 gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca
      721 ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact
      781 tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt
      841 gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct
      901 accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact
      961 gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt
     1021 ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa
     1081 tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc
     1141 tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa
     1201 taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc
     1261 atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct
     1321 gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg
     1381 aacatggtgg attctccatt cgttatttat tttaatacat catttagagc ttcactcatt
     1441 gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct
     1501 agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc
     1561 aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt
     1621 gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg
     1681 gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc
     1741 acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt
     1801 cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc
     1861 caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt
     1921 agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca
     1981 gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca
     2041 gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc
     2101 gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat
     2161 tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat
     2221 tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc
     2281 atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg
     2341 attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca
     2401 ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc
     2461 ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt
     2521 tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat
     2581 ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca
     2641 acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt
     2701 ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac
     2761 ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt
     2821 tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt
     2881 tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga
     2941 gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc
     3001 atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag
     3061 agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg
     3121 taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc
     3181 ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac
     3241 ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact
     3301 gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg
     3361 ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa
     3421 aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa
     3481 tcttttctac ataatctttt atatgattga ttttaagata catatatacc agaaagttga
     3541 ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt
     3601 gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact
     3661 agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg
     3721 aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc
     3781 tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat
     3841 ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt
     3901 tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca
     3961 tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt
     4021 tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg
     4081 atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt
     4141 tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca
     4201 tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt
     4261 agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg
     4321 attgtactta atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca
     4381 actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa
     4441 acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat atgattagga
     4501 aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat
     4561 actttttcag caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca
     4621 tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc
     4681 gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt
     4741 tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag
     4801 aattttattc cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg
     4861 cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa
     4921 tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt
     4981 ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat
     5041 ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc
     5101 gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt
     5161 tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca
     5221 gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta
     5281 caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca
     5341 catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca
     5401 aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt
     5461 taaataatgt atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc
     5521 gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa
     5581 gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc
     5641 gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata
     5701 tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct
     5761 ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg
     5821 ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat
     5881 gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg
     5941 gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt
     6001 ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg
     6061 atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct
     6121 tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc
     6181 caattttttt taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt
     6241 cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta
     6301 ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca
     6361 attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt
     6421 tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa
     6481 gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt
     6541 tataatagaa atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa
     6601 caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc
     6661 gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta
     6721 acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt
     6781 ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat
     6841 ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg
     6901 tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt
     6961 atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca
     7021 acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca
     7081 tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat tatcggctgg
     7141 tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta
     7201 ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc
     7261 agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa
     7321 aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt
     7381 ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat
     7441 ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc
     7501 gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg
     7561 aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc attattccta
     7621 ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt
     7681 atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta
     7741 caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact
     7801 tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg
     7861 tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc
     7921 tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca
     7981 ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta
     8041 tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag
     8101 atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta
     8161 gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg
     8221 ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga
     8281 tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg
     8341 ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac
     8401 ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg
     8461 gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa
     8521 aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca
     8581 tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac
     8641 ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc
     8701 tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg
     8761 gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa
     8821 acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta
     8881 cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag
     8941 gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa
     9001 gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct
     9061 atctattagc caacttctta aatactggtc attggcctac ggaagattat caaaaggaca
     9121 tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc
     9181 gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa
     9241 tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt
     9301 caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct
     9361 caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg
     9421 acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt
     9481 tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca
     9541 aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag
     9601 caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg
     9661 atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga
     9721 taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa
     9781 gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc
     9841 gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct
     9901 gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa
     9961 agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt
    10021 catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc
    10081 cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt
    10141 tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca
    10201 aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga
    10261 gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa
    10321 agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg
    10381 aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa
    10441 tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt
    10501 aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt
    10561 gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc
    10621 catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga
    10681 ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc
    10741 tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac
    10801 tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca
    10861 tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt
    10921 ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag
    10981 aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc
    11041 gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg
    11101 ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa
    11161 ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg
    11221 tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg
    11281 agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt
    11341 tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt
    11401 tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc
    11461 agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga
    11521 atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat
    11581 tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt
    11641 aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa
    11701 tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt
    11761 gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa
    11821 agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc
    11881 actgcaataa tccataaaaa aaagcaaaaa aatttt
//
LOCUS       DACDOA010000080        11584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDOA010000080 DACDOA010000000
VERSION     DACDOA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.080"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            91..3318
                     /gene="fss3"
                     /locus_tag="IWI39_002051"
     CDS             91..3318
                     /gene="fss3"
                     /locus_tag="IWI39_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9209144.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3430..3744
                     /locus_tag="IWI39_002052"
     CDS             3430..3744
                     /locus_tag="IWI39_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9209145.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3757..4131
                     /locus_tag="IWI39_002053"
     CDS             3757..4131
                     /locus_tag="IWI39_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9209146.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4141..4485
                     /locus_tag="IWI39_002054"
     CDS             4141..4485
                     /locus_tag="IWI39_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9209147.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4554..5570
                     /locus_tag="IWI39_002055"
     CDS             4554..5570
                     /locus_tag="IWI39_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9209148.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6164..8038
                     /gene="ltrA"
                     /locus_tag="IWI39_002056"
     CDS             6164..8038
                     /gene="ltrA"
                     /locus_tag="IWI39_002056"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9209149.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8164..8520
                     /locus_tag="IWI39_002057"
     CDS             8164..8520
                     /locus_tag="IWI39_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9209150.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8565..9707
                     /gene="dcm"
                     /locus_tag="IWI39_002058"
     CDS             8565..9707
                     /gene="dcm"
                     /locus_tag="IWI39_002058"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9209151.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9697..9986
                     /locus_tag="IWI39_002059"
                     /pseudo
     CDS             9697..9986
                     /locus_tag="IWI39_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10242..11426
                     /locus_tag="IWI39_002060"
     CDS             10242..11426
                     /locus_tag="IWI39_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9209152.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11441..11536
                     /locus_tag="IWI39_002061"
     CDS             11441..11536
                     /locus_tag="IWI39_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9209153.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3888 a         1984 c         2576 g         3136 t
ORIGIN      
        1 gtcaataaat attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
       61 tttgtgccta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
      121 gtattaacta ttttgagttt ggctgttcaa tcaattgtag gagcaattgc tcctacgatt
      181 gcttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
      241 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
      301 cattatgcag tgtacaacgg cgttaaacaa gatattttct gtgttgaacc aggagtcccg
      361 atttacaatg agttcactcc tggatatgag aagaacccgt taccagatat gtccgataaa
      421 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
      481 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
      541 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa tcaatcaagc cattgccgat
      601 tatcagaaga aaccaagctt ccataatagt acggctaaaa cagtattagg gcaatcgact
      661 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatacc
      721 gcaaacattg attatcgtgt gaatggcaat cagttgataa tcagcccaaa tgccaattca
      781 aaagaaagtg gtgtgttgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
      841 aaaaaggctg gccaacagac tttaatggcc ggggcgatcg ataagccgaa cacttatacg
      901 gtcaaaattg atgtggaaat ggagggttct ttgaagatta aaaaagttga taaagaatcg
      961 ggtgatatta taccaggaac ggttttccat ttagactttg gaaaaacttt acccgcaaaa
     1021 gacgtgacca ccgataaaga aggcattgcg accttagatg agattcctca tggtacaaag
     1081 gtaaccatta ctgaaaaatc ggtgccggcg ccttatacga ttgataccac acccatgact
     1141 gccacaatta aagcaggtga aaccatttca gtgacttcaa aaaatacgcg agaaaaaggt
     1201 caaattattc tggataaaac cggaattgaa acagggagtg atctttggaa cgataattac
     1261 tctttagcgg gcaatacgtt tgccattcgc aaggatagcc caactggtga aatcgtccaa
     1321 gaaatgacca cggatgaaaa aggtcatgca gaaacaccaa aagagattgc caatgcgttg
     1381 gaattgggaa cctactatgt gactgaaacc aaggctagtc atggctttgt gaatactttc
     1441 aaaccagtaa aagtcgaatt gaattatgtc aatcaaacag tggctcttgt taccagtaac
     1501 gtaaaagggc aaaaccaaga aattactggt gaaaccactt tgacaaaaga agacaaagat
     1561 accggtcatg aaactcaagg gaaagctgag tttaaaagag ctgaatacac cttgtttact
     1621 gttaaggatg gaaaagcggt caaatggacc gagaacttta aaccagaatt gttgaaggga
     1681 acgaaagctt ctgatgaaac cgtaactttg actttagatg aaaagaatca agttgccgtt
     1741 aaacacttag cgattaatga gtattactgg caagaaacca aagcgcctga aggatatacc
     1801 ttggatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgct
     1861 gtgattactc gagatgttgc ggcaaaagaa caagtgattc gctttggctt tgatttcttt
     1921 aaatttgctg gatcaactac tggcactgcc gaaactggct tcaatgatct aacctttaaa
     1981 gtgtcgccat tggaaggaac caatgaaatc accggtgccg aagatgaagc gaccacagct
     2041 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
     2101 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
     2161 cgttctactt ttaaagaaaa taaagaggat tataccaaga gtgattatat ctttactatt
     2221 accgaaaaag ggcaaaagca accgattaaa acggtaacgg tcccttatgg gaatctaacc
     2281 aatgagaatt tctcagtcag tttgaaccgt ttgatgcttt atgatttgcc tgaggaagaa
     2341 gatagcttaa cttctcttgc gacttggaaa gaaggagata aagaattgac tactcttgat
     2401 tctaccgagc tagtcgataa attgagctat aatctacatg aaatcaaaga agactggtat
     2461 atcgtagctc aagccatcga tgtagatgct acaaaagctg cccaagaaaa agataaaaaa
     2521 gccaaaccgg tggcgattgc ggaaacaacc gcaactttag cgaataaaga gaaaacagga
     2581 acttgggaga ttctgcataa attaactgtc gaacaagttt tagacaagac cattgtctta
     2641 ttcaactatg tgtatgaaag caaagaagcc tatgaagctg gtgatgagcc ggtagcgaag
     2701 gatgctagct tgaacaatca agcgcaaact gttaattgta tggtggaacg tcatgtttcc
     2761 atccaaacca aagcgcactt agaagaaggt tctcagacct ttactcatgg tgacgtggta
     2821 gatatgtttg atgatgtatc cattacccat gatgtactag atggctcaaa agaagctttt
     2881 gaaacaattc tgtacgcttt atttccagat ggtacaagta aagaaatttg gaaatctggc
     2941 aagattgatt acgaagtgaa tgacaaagaa tttaccaaaa ccgtacttgc ggaaaaagtg
     3001 gatacaagta aatatccaga aggtacaagt tttaccttca aagaaattaa ctatgacaaa
     3061 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aactttaacg
     3121 cccaaaaaag ttccgacaac accaagtaca ccagaacaat ctgaaatgcc aactgttcca
     3181 agtggctctc aagaatctag tcctacagtg aaggcattcc ctcaaaccgg ggagaaaact
     3241 tccaatgtgc tactgttcat tggctttagc ttgatctttg cgacggctgg ctattatttt
     3301 tggaatcgtc ggaactaagg tgataagatg aaacatgtaa atagtagaaa acaatacgcg
     3361 cgaccgcctc ctaaaaaagt ggtcgcgcta tttttgtatt caaaactaac aatgaaaaga
     3421 ggaaaaaata tggaattgaa atttgttgta ccagatatgg tcgagacgtt cggaaaacta
     3481 acatacggtg gtgaaggtga agttctatcg gaagggtatg gtcgaaatac tacaattgtt
     3541 ggtcgaagtt accatctgtt ttcaaataaa caacgggccg atgatattga agtggtggta
     3601 gctgcggaag ccggcgaaaa ggattttgac caagatcaac cactaaaagc tgtaaatccc
     3661 cacttaattg ccaaaggcta tgaaattgag aatcgtgggt ttaccgatta cgtgttgtat
     3721 gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc
     3781 gtagattcag gattaacctt tggcaagttg cgtttttccg cattacgtcg agaagtacgc
     3841 aagcaaaatg aggacggaac ggttagtaac gaggtaaaag aacggaccta taatttgaaa
     3901 tcctccgcgc aaggtcgaat gatccaagtc agtattccag caaatgtacc cttacgcgag
     3961 tttgcttatg atgcagaggt agagctggtc aatccaatcg tggatacggt cgccaattat
     4021 gtttttcggg aaggaaccac cgttaattgg tttatcaagg cagatgattt agtcttgaaa
     4081 cgacaactga atcaaggaaa acctacgaac caaaatgaag gaaagaaata ggtgaagaat
     4141 atggaaatta atgttgaaca tattttagat tgtctcgatc aatttggcaa aggtgagcta
     4201 acggaggaac aactcactaa cgcattaacc tatgatgaaa aaatgtttct gattatgcat
     4261 caagggttac tggaagtagg taacgatgcc aaagaagact ttgatgtact ggattggtta
     4321 ggggaagaag agtctttctt tatggttgtc gaggtaaatg aaagtttatg tcggcaggca
     4381 gaatctgttt tggaagaaat cgatgtggag atgccggatg ccatcgaaag atttttaaaa
     4441 caattagtgg aaacgaaaca acttcccgta gtagtaaatg actagcataa ctttttgaaa
     4501 ttgcccaatg aaatcctatt gggctttttc gactggaggt ggaaaccatc aaaatgtaca
     4561 aaggacatcg catacgcgca ggagatcaac acttgattta tcactttgtt cttggctggc
     4621 taatcttttt gttcattggt tggatgagcg tcttttattt acaagaactt agacaatttg
     4681 atatttctaa ggtatcattt tccactagtg aaattggctg gtctatgaag gatgtaatct
     4741 gcttgctagg aagtctcgct ctttcaggaa gcatgatgtt actttatata cacttttttc
     4801 aggatcattg gcgaagtctt tggcatcgac aaaagttagc tcggatgatt ctagaaaacc
     4861 attggtatga agtaaagcag actcaaggtg aaggtttttt caaagatttg aatagtagtt
     4921 ggaccaaaga gagtatcagt tacttcccca aaatttatta tcgaatgaaa gatggcttac
     4981 tttctattcg cgttcaaatt tcactgggaa aatatcagga tcagctattg aagttggaaa
     5041 ataagttaga aagcggactg tattgtgagt tagtagaaaa agaactcaaa gactcctatg
     5101 tggaatacac cttgttatac gatatgattg ccaatcgaat tgggatagaa gaaatagtgg
     5161 cagaaaatgg ggcgttgcga ttgatgaaaa atcaagtatg ggcgtatgat tccttgccgc
     5221 atatgttaat tgctggcggc acaggtggtg ggaagaccta tttcttactc accattattc
     5281 aagcactatt gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcggacctcg
     5341 ccgatttagg tacgataatg cctcacgtat actcccaaaa agaagaaatt tctgaatgtg
     5401 tggaagattt ttacgaacgc atgatagctc gtagtaggtc aatgaaagaa atgcccaact
     5461 acaaaacagg agagaattat gcgtatctcg gacttccgcc caacttttta atctttgatg
     5521 aatacgtggc ttatatgggt gcgaatcgtt ttcctacaag catcgaatag atgtgaaatg
     5581 gaagcctatt gagattatca atagtgacaa ctaatgtttt gagagatgga atgaaagcct
     5641 tcaagttgag ctgaaacatc cccgctaata ctcctacatg ctctaatcaa tgaagtagat
     5701 tggttttggt atgaagctag gtgaagtcgt ctgaaatctg cctaagtgct tgagcataag
     5761 gctgactata gggcgggcgg ctgaaaaaga taactatggt aagaatgtct gaaaatgact
     5821 gacgaatctg cgaatgtacg ggtctaaaag gctgatctta ttgaaagata ggaacatacg
     5881 gacgtatggt gagataccga caagtaaggt tccatgatat gaaaatcggg atagctaacg
     5941 aagaagctat aatttcacag gctcatagta gatacctaag gttatacatg atagctaaaa
     6001 acattggaac gatgtaagca gaaaactatc aattaaatga ctacttctag ataggtgcat
     6061 ataagtaata acgaaattgc tttgtttctg tgaaagtaag ggcacagtac cgatgaagcg
     6121 tctaacaaat gtggagggat agccctaagt ctatttaata acaatgaatg ttgaaacaga
     6181 tgaagttcaa ggatctggta agaacgtggg aaaatccgtt atggaggtgc cacgcttgaa
     6241 cacaaaaata cgttactggg aatattatga tctacaagaa acattcgatc agctatttac
     6301 tcaaagtaga aacgggaaaa agttttatca attatatgag ttaatcattt ctgaaaataa
     6361 tattctttta gcataccgta ctatcaaggc aaacaagggg tcttcgacac caggaacgga
     6421 ttccttcaca attgataact ataaggaaat gaaccaagca gagtttatcc atcttattct
     6481 cagtcattta gaaaattaca aaccaaaatc cataaagcga gtaatgatac ctaaacccaa
     6541 tggtgagaaa cgcccattag gaattccatg catgattgat cgaatgatcc aacagatgtt
     6601 caagcagata ctggagccaa tatgtgaagc taagttttat gaacatagct acggtttcag
     6661 accattgaga agtgcaaagc atgctctagg tcgcattatg tatctcataa acatcagtaa
     6721 aatgcactat gcagtagaca ttgatatcaa aggtttcttt gataatgtaa accaccgttt
     6781 actgataaaa caattatgga atataggaat ttgtgacaaa caagtcttag ctattctttc
     6841 aaaatctctg aaatcaccca ttcaaggaga aggtatcccg agcaaaggaa cgatccaagg
     6901 aggtatcatt tctccactat tatctaatgt tgttctaaac gacttagacc attgggtctc
     6961 aaagcagtgg catacctttg aaacaaagta tccctatact aaaggttaca ataaatttcg
     7021 tgctctaaga gatacaaatc taaagcaagg ctatattgtc aggtacgcag atgactttaa
     7081 aatcatgact aatgattatc cgacagcatt aaaatggttc cacgctgtca agctttacct
     7141 aaaagacaga cttaaattag atatttccaa tgagaagtct aaaatcgtca atttgcgtaa
     7201 atgtaagtcg gaatttttag gattcgctat ttgcgtgaaa caaaaaggga aaaaatgggt
     7261 ttgtaattca cgtatttcta acaaaaaaaa ggatcagatt aaagaagaga ttaaacaaag
     7321 aatcaaagat attcaaaaga gtcctacagc acaaaatgct ctcctgttta atgctctcgt
     7381 gctcggtgtt cataattatt tcaggtatgc cactcatatt aatttagatt tgaataggat
     7441 gaactacgtt ctttcattca ctacttacaa ccgattatca aattgtagta caagatgtta
     7501 ccccaacaaa gcttccccaa cttacaaaaa gttttactca acaacgatga aaacgtataa
     7561 agtagctgga gtctatttgt accctctttg cgacgtatct acaaaaacaa tttacggatt
     7621 taatacggag gatacaccgt ttactccgtt tggtcgtcaa agattggagc acaaaagcct
     7681 acagtctctt gtgttccaag aattaagaaa attgatggaa tcgaaaatac cgaatagaac
     7741 ggttgagtat ttagatagta gaatatctcg ttactcaatg aaatcaggca agtgtgagat
     7801 tacaaaacaa tttttgccag caaaagccgt tcactgtcat cactatttgc caaaaagtct
     7861 tggtggagac gacaaattcg ataacttgag aattattcat aaggatatcc atcttctgat
     7921 ccatacaaca aataaaatga taatcgatca ctacgtgcat gaactgaaac tggtgccaga
     7981 gcaaatagca aaaatcaacc tttatcgaaa gatgtgtaat ctgcaaaaca ttcaatagaa
     8041 aataaataga tggaacgccg tatgaggtga aagtctcacg tacggtgtgg agcgggggaa
     8101 aagatggaga ttatatcaaa ttcttaccta tcgctataga tgttaacgac aaaagaaagt
     8161 gcggtgattt tgaataagct taaacaaatc gtgatgttag gccgtcaatc tggtttcttt
     8221 ctgattctag cttgtcaaag gccggatgcg aaatatctag gagatgggat tcgcgatcag
     8281 tttaacttcc gtgtggcttt gggtcgtatg agtgagcttg ggtattccat gatgtttgga
     8341 gaagtcgata aaaatttttt tatgaaacgc atcaaaggac gaggttatgt agatacaggg
     8401 ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttaagg
     8461 gaaattagca acattgttgg attaaccgtt catacggaaa gagaaaataa tagtgtgtag
     8521 cctaggaata tcaataatcc tgggcttttt tattgaggtg atttatgaca tttttagatt
     8581 tattcgccgg gattggtggc tttcgcttag gtatggaaca agccggtcac cagtgtatcg
     8641 gtttttgtga aattgatgaa tttgctcgga ggagttataa agcaatccat gatacaagga
     8701 aggaggtgga aatgcatgac atcacaagtg tatcagacga atttgttcga tccctcggac
     8761 cagtggacat cctttgcggc ggctttccgt gccaagcttt ttcaattgcg ggaaagcggc
     8821 aaggattttc cgatactcga ggtactttat tctttgaaat cgctcggttc gccactcttc
     8881 tccaacctaa gtttctattc cttgagaacg tccggggatt gctcaatcac gaaggagggg
     8941 ctacgttcga gacgatcctc cgaacgttgg atggattggg gtatgatgtg gaatggcaag
     9001 tgcttaactc aaaggcctac gttccccaaa gccgtgagcg gatcttcctt atcggacatt
     9061 ctcgagatgc atgtaccgaa caagtatttc ctatcattgg atcgtcttca acatctgatc
     9121 aaaacatcag aaacttggta aatatcaatc cttccaatcg aggaatgggt ggccaagtct
     9181 atggttcaga tagcgtagca ccaaccttga ccggtgatga aggaattaaa attgccttac
     9241 cggtaaagga tgggatttct gtagcgggta tgttgtcggg aaattttgaa caaggcaata
     9301 ggatttatga ggtaaccgga acagccccca ctttgtcaac gaaacaaggg ggaacgaaga
     9361 ttatgattcg gaaaaatgaa acagaatatc aagaggtgaa gccaggggac agtgtgaacc
     9421 tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacaaagt gtgcatacct
     9481 tactaacagg agaccaacaa gcggtagtca cggatcagta ccagattcga aagttaacac
     9541 ctagggagtg ctggcggctt cagggctttc cagattgggc ttttgatcga gcttctcaag
     9601 tcaattcaga tagtcagttg tataaacagg ccggaaactc agtaacagtt ccggtcattt
     9661 ttgatattgc tagaagatta aagggggtaa aagcatatga tttatgacga gttgattgga
     9721 gaaatctact ggatgattgg caagattcag gcagatccga aattggaaga agaactgtac
     9781 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc aggtgattcg tatgtagtgg
     9841 atgaggaaac ggatgctcga atggagctca atcaagtcat cggtgaattg gaacgaattg
     9901 cggaccttgc caaagaacct gatatccgcc aatacttgtt tgagataaaa gcagagttgg
     9961 aaattgaggg tatcaccgcg gagtaatagg gaaataaccc actgataggc actgaccgcg
    10021 ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcggcagcc
    10081 ggccatgatg tctcaccccc cgtatctaac aggggggtac aaaaacacaa aaaacaagca
    10141 agtaaccata aaaaatgctt gttgtatcaa ggatattaag taatttgtat aggtgttaac
    10201 tcatttggaa aagttaacac cttgaatttg gaggtgaaaa cttggcgcaa cgaaatttag
    10261 attatcggtt attaaaagaa cgacgcaacg aatatggcgt ttcacaaaat aaattagcta
    10321 ctgcttgcgg acttagccgc ccatacctaa atcagataga aaacggtggc gtaatcgcct
    10381 ctacaaagac catgagaaaa atttttgatc aactggaaag tttcaatccc gatttacctt
    10441 taacgttact atttgactat gtaaggattc gctttcccac aacggatgtg cggaaaatta
    10501 ttcaagaaat tctccatttg aaattcgagt atatgcttca tgaagattac gccttttact
    10561 cctatcaaga acaatatgtc atgggcgata ttgtagtgat gttatctcat gaagaagata
    10621 aaggtgtcct tttggaatta aaaggtcgtg gttgccggca gtttgaaact tttttactcg
    10681 cccaaaagcg tagctggtac gacttttttg aagattgtct aaaagccggt ggcgtgatga
    10741 aacgcttgga tttagcaatc aatgatcgag tggggctact ggatattcct gatttaacga
    10801 aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagt taccgttccg
    10861 gggaactgtt gaaagctgat gaaaaggatg gtatgggcaa taccttatat attggcagcc
    10921 tcaaaagcga ggtttatttt tgtttgtacg aaaaagatta tgaacaatat atcaagttag
    10981 ggattccatt ggaccagacc gaaacgaaaa atcgttttga gattcggttg aaaaatgacc
    11041 gtgcttatta tgcgattcaa gatttgttaa aaggtcgtag cattgaaagt accacgtttt
    11101 ccattatcaa tcggtatttg cgatttgcgg ataaagtaga tggaaaacgg cgaaccaatt
    11161 ggcccttgaa tgaacaatgg ggtcgtttta ttggacggaa tcggaaagag attcaattaa
    11221 cttctgaacc gaaaccgtat accattgaac gaacattaaa ttggctaggt agacaagtcg
    11281 cccctacgtg gaaaatggct aaggagttgg atcgattgaa tcaaacaacg tatatccagg
    11341 atatggtaca gaatgcgcga ctctcggacc ggcataaaaa gattttggag caacaaagta
    11401 tggcaatcga aaacttgatt gtatgaaaag aggaattttg atggaaattg taaaagatat
    11461 gttattggta cttggtggca gtatgcttgg tgttaccgtg atgtgcttga tgaatgcaag
    11521 tgcagcagcg gattgagcaa tggaaaagaa aggaaagtga caaaatgaat ttcggacaaa
    11581 attt
//
LOCUS       DACDOA010000081        11397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDOA010000081 DACDOA010000000
VERSION     DACDOA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.081"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..307)
                     /locus_tag="IWI39_002062"
     CDS             complement(<1..307)
                     /locus_tag="IWI39_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209154.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     "
     gene            complement(607..1488)
                     /gene="galU"
                     /locus_tag="IWI39_002063"
     CDS             complement(607..1488)
                     /gene="galU"
                     /locus_tag="IWI39_002063"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9209155.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1531..2550)
                     /locus_tag="IWI39_002064"
     CDS             complement(1531..2550)
                     /locus_tag="IWI39_002064"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9209156.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3183..4187)
                     /locus_tag="IWI39_002065"
     CDS             complement(3183..4187)
                     /locus_tag="IWI39_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209157.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4200..5249)
                     /locus_tag="IWI39_002066"
     CDS             complement(4200..5249)
                     /locus_tag="IWI39_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209158.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5266..6087)
                     /locus_tag="IWI39_002067"
     CDS             complement(5266..6087)
                     /locus_tag="IWI39_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9209159.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6074..6853)
                     /locus_tag="IWI39_002068"
     CDS             complement(6074..6853)
                     /locus_tag="IWI39_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209160.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6870..7340)
                     /locus_tag="IWI39_002069"
     CDS             complement(6870..7340)
                     /locus_tag="IWI39_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209161.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7352..7771)
                     /locus_tag="IWI39_002070"
     CDS             complement(7352..7771)
                     /locus_tag="IWI39_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9209162.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7852..10620)
                     /locus_tag="IWI39_002071"
     CDS             complement(7852..10620)
                     /locus_tag="IWI39_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209163.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10820..11044
                     /locus_tag="IWI39_002072"
     CDS             10820..11044
                     /locus_tag="IWI39_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209164.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3275 a         2413 c         1871 g         3838 t
ORIGIN      
        1 cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta
       61 cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca
      121 aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc
      181 tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat
      241 tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt
      301 ttcccatagt gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta
      361 tgaatcttct ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa
      421 aaaaaaatta aaccattagc ataatatttt ctgaatctat tcacgaaagt ctttttctat
      481 ctttcgtttt ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa
      541 atgacgcaca agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat
      601 ttccagttaa ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga
      661 agtcccaatt tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt
      721 atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt
      781 aagagtctga agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc
      841 taatatctca aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg
      901 tgcttttccc atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct
      961 atctgtttta ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac
     1021 ggcaacagcg gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat
     1081 atcatctttc atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc
     1141 ttttgcttga agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag
     1201 attgacttga gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa
     1261 attgttttcc aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc
     1321 cgttacgatc aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat
     1381 cgttggttta tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa
     1441 acgtgttcct agcccagcgg caggaatgac tgcttttttg accttcactt gctgttcctc
     1501 ctaaacgacg attaactatt gatcatctat ttaacgaaga gaaaattcat tctccatttt
     1561 tccgtcacgt aacatgattt cttttgctgc ttttttgata tcttctccat tatacaaaac
     1621 attatagatt gtttgagtaa ttggcatctc aacacctagc tgttgagcta gctctactgc
     1681 tgcttttgtc gttgccacac cctcaacgac cattcccatg ttttctagaa cttcttccag
     1741 cttatgtccc tgtcctaaca agttaccggc acgccaattg cgagaatgaa cacttgtaca
     1801 agtaacgatc aagtccccca caccactcaa accgataaat gtcagaggat tggcacccat
     1861 cgcgacacca agacggctga tttctgccaa tcctcgagtc atgattgctg cttttgcatc
     1921 atctccgaat cctaatccat ggatagcgcc ggcaccaatt gcaatgatat ttttcagtgc
     1981 tgcaccagtt tctacgccaa tcacatcatt atttgtataa atcctgaaat aatcattcat
     2041 gaacagtttt tgtacatagg ctgctgcttt ttgatcaata cttgcagctg taattgttgt
     2101 gatatcatgt actgccactt cctcagcatg gctaggacca gataatacaa caattgcctg
     2161 tcttttttct tctggaattt cttcagccaa aacttctgag attcgtttat gcgtatcttg
     2221 ttctagtcct ttacttgcat gtataatgat tggcttagta ttcattttac caataagttc
     2281 ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat aaaatagcat ctgcgttatc
     2341 cactgcttct gccaattctt tatatccgat aatcgattct gggatcgtca aatcaggtaa
     2401 aaaatgctga tttgtatgat acttattgat ttcatcaatc tgttcaggta catttcccca
     2461 aatacggaca tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc cccaagatcc
     2521 aggaccaaga actgcgattt tttgtttcat ggatattcct ccaaataaat aattgataat
     2581 ttccattata gtatacattt cttaaagttg ggactaattt ccattgtttt taagtgtttg
     2641 tttacttctt ttatcgtttt tacttgaaat aatcaatgat atgttacaat agtctacatt
     2701 ttatttttac actcgttatg ctttgcttgt tcctaagagg aagcagtttt gacaatgtta
     2761 ctaccagcat tactattagg aggttaggaa acttctccta gtgagtattg atttaaaata
     2821 cataagagac agattgaaca tttctaaatc tgtcaggtcc tgcaacggac agattgtctc
     2881 tgttgacggt tgacatagga acttttgttt tagtcaacat tctggataca aaacgctatg
     2941 taaactggcg caactcgttg tacttggact tttactacgg atccatgcaa caactaaata
     3001 gtactattgc ataacaaaaa atggttggca cccacatgtc ccattaaaca gattataagt
     3061 tgaactgtag ataatgcaaa aaatgtgttt tataacgtaa ttgatgccaa tattttaaaa
     3121 aaataaaggg agcagttatc gctgctctct ttatttttta tattcttttt atccagtcta
     3181 tcctaataat caaactgacg atagtagcgg cgtatctcta atggatggca gttcaactgc
     3241 tccacatgag catcgatgcg ttgtgtcact acatgcatca ataagaaaga caaaacacgt
     3301 ccgcgatatt tttcactgat accttctact ggatagtctt ttgtgtctat caatgtataa
     3361 ttgctgcaaa tttttggcaa aagtttagcc acacgttcag ataacactct ctgttcatct
     3421 tcgcctaaaa ataatgtgac tggggttgtt tcttctacga tctctaacgt cccgtgaaga
     3481 aactcggcag catggataga tttcgatgga agccagcttt gttcttccca gtaacacatg
     3541 gcatatgaat aaacagctcc ccattgattc cctgctccaa taaaataatg catactatca
     3601 cgtcgatgtt tttctgcaaa tgataagcca aatgcatctg cttttttctc tgcttccact
     3661 aaccctgttg gtaaataacg ttcgagctgt tcatagtatt ctgaataagc ttcgaattct
     3721 ccattcaaat acatcaatcg atctgctgcc ataaaaaatt tgatttgttc tgttccagga
     3781 gctggataag tgaccacaaa atcgcatagc tgagacaact tactgttcgc tttatcgata
     3841 aatccaatca gtgtcgctcc tactttcttg atttgttcta ctgctttcac tacttcttga
     3901 gttgtaccgg taacagagga caaaataacg atagaatctt ttgttaatct tttgtttcca
     3961 gtagtgtaat attctgcagc atgctgtaca aagacaggca agttgctttt cccattcata
     4021 taagtgacag cttgcatcga ggaagcatac gttccaccga ttcctagata atagacagcg
     4081 tcaaattttt ccgcataaag tttatcgatg atttcttcta cttgcggacg taaagctagt
     4141 gcgcctttta ttgctttgat ttgttcttct tcattgaatt tcagcatgat ttccacctct
     4201 tataatttac ttttcaagac tgtatcaaag tcttcaaaaa tctttttgtt caagtcaata
     4261 cctttagcta ctgcggtttt ataagcgaat agttgaaacg gaacgacaag cgctaataca
     4321 gacagatatt catcacattc tattgctaat ccaagtgttt tctcgtttct tgcttttttt
     4381 gtggtaatgg tgagagtctc tccaacgtac cgactcatat atctttgcaa cttttgcgaa
     4441 cgttccttat ttacactttc cccttcaata aagaatagca aatgcgaagc atctgcctcc
     4501 aggtatgggc cgtgcatata agcttctaac tcaaatcctt gtgacggaac gcggattgtt
     4561 tcagtcaatt tcgtctctgc ttctttagct gttccccagt ttggaccata tccaatggtg
     4621 acgaaacgct gtgctaaacg aaataaactt tgatatccat caaaaaattc ttccgttttt
     4681 tgaataatcg ctggtaaatg gttgattatt ttgcgcaact gttgcatgta gtcttgttct
     4741 atttttttgc tgattttatt tttacttatt ccaattccta tccctaagag tagtaactgt
     4801 aaaacagtcg cagaatagcc ttttgtaacg aagcctactg attctacccc ggcacctaaa
     4861 tcaataattt gtgcagcttc taaagcaagt ggactttgca cgtcatttgt gatagcaaca
     4921 acaggtaacc cttgtttttg tatcattttt actgcttcaa cagtagaagc acttttcccg
     4981 ctttgtgaca cagcaatcac agtatcgatt gaaggagaaa gttttccata attgaaaaaa
     5041 taaaaaggtt cttgaatatc gacagtaaga tcaccataag attccaatgc tggcttcgct
     5101 gctaaacaag cgttataaga tgaacccgtt gctaagatca gtaaattcac tgtttttttc
     5161 atatgttctc ttgtcgaaaa atcattcttt ttcaagatcg tacttagtac ttccttttct
     5221 tcatagatat aatcttgcat cgtaaacatt ttctctgctc cttttttaaa aaatccctat
     5281 ccacgaacca aaaattccaa agaaagctag tccgatcaag atcgttaatg gtttcacttc
     5341 tttcttcaat agataaaaca ctatgccaaa tgctccaaga cataagatat ttggaacgat
     5401 atcatcaaaa atactttgaa cagtcacagc attttctcct gaaccgattt ttaaaggaat
     5461 attgatggtg atcatcgtgg cagtcattgc tccaacgacc atcagtccaa taatagacgc
     5521 gcctaatgtc aaactttcca tcataccgtt tgcctggatc tttttcaaaa aacccgttcc
     5581 taagcgatat ccccaacctg ttgctagata gcgaaacaga atgtgcggaa tattgaaaat
     5641 caaaatgaac aagatagggc ctaaaatatt tccttgcatg gctaaagagg tacccacacc
     5701 tgtggcaatc aatcgcaaag ttccccaaaa gaaagaatct cctaaccctg caagaggtcc
     5761 catcaaagat gttttgatac tatcgatcgc agtcacatca aattcaggat catccttatt
     5821 ttcttcctcc attgcagccg tgattcctaa aatcagtgta acgatatgtg gggtcgtgtt
     5881 aaaaaactct agatgccgtt tcaaagctgc tgccatttct tctttcgact gatataactt
     5941 tttcaaaatc ggaatcattg cataaacata agccatattc atctgacgtt cataattcca
     6001 agaaaactcc atttgaaaag aacgccaaaa tacgtgattt agctcttttt tcgttatttt
     6061 gctgtttttg atctcagaat tcttcatctt cttctacctc ccctgcagtt gtttgaagtg
     6121 gtagttgacg gacattcata atattgacta ccacaactgc taaaatcgca ccaaaaatag
     6181 ctactccggt cacaggaatc tctaagtaag aagctatagc aaaacctaag aaaaaatatg
     6241 gtgcaacttg tttgttgatc agcaaacgag caagcatcgc aaaaccaaga gctggaatca
     6301 accctgtagc tactgacaac cccttttgaa cgaagtcagg aatcatattt aatagatttc
     6361 cgatcgtatt gcttcccacc atataagaaa ccattaccac aacaccaagc atcaaggaaa
     6421 gaccgatacc ggcaagcaag tgcatatgct cgactccttt ataatttcct tcttctgcat
     6481 aattgtctgc tttatggttc ataattggaa tcaaaagccc taagtaaata tttttcaata
     6541 tcaatgtcaa cgtagcaatc ggcaaaccta gtaaaagcgc ggtttctgta ccggctccag
     6601 ctgtaatagc aaaagccgtc cctaaaatcc caccggtcac aacatcaggt ggaatcgaag
     6661 cacctactga gaacgatccg ataaatgcta actctaaagt tgcgcccata atgatcccag
     6721 tttttacatc acctaacacg attcctgtaa acagccccgt cacaatcgga cgagaaagca
     6781 acgatgtccc tagtgcatat tctgattgtg cgacaaatgc aacaattcct aataagactg
     6841 cttgtacaat cattgaggtt cttcctttct tacaaaactt ctcttacatc cactttttta
     6901 tctgctggta cttgacggat ttctacttct atttgtttgt caattaatcg tttcaagcgt
     6961 tcctcctctt ctggtaaaat atttaccgct tttccgatgc tgcgagtacc aggttttgcc
     7021 ttgatccctc ctaagttgat ggttttgatt tctggatacg cttctgccag cttatatgca
     7081 tcctcgatcg attccactac gatgaataac ttatatttat ctgtcacccc gcttttcaac
     7141 gcggcaatcg agtcatcaat tgatttgatc actagtttga ctccttgtgg cttggctaat
     7201 ttgatcgttg tctttcgtat ctcattcgta ggtacatcat cattagcaat caaaatacag
     7261 tctgcgccta ttgtttgtgt ccatgaaaaa gctacttgtc cgtgtaataa ccgatggtcg
     7321 actcttgtca atacaatcat ttttcttcct cctaaaattc gtcttcttct attttctgat
     7381 tgagccgctc gttacaaaac tggatcattt tttttgaatc agttagcacc tgctggatca
     7441 aaacagttgt ctctacttca gcgtccaacc gagatactag ctcaatcaat agtggcaaat
     7501 tcaatccggc aacgagatag acatgatccc gattgatata ttgtaagaat tcgttgttga
     7561 cacttccgcc aaaaagatcc gtcagaacaa tgatttcttt atctttgtac ctcataaaaa
     7621 gctgccttac tgtttccgct aaatcaaaat tctcatcagt atagcaatcg aatacagtaa
     7681 tagaatgatt tttcccatag atcagctcta atgaatctaa tatccctgaa gcaaattttc
     7741 catgggaagc taagataatt tctctatcca ctttttcatc tcctcttttc acttacacat
     7801 actaaaaaag caagttgcat gccaaaatta gtggcaacaa cttgcttatg ttcaaaattc
     7861 ttcttctatc gagagaaact ctgtatcttc actcaaaata tcatagatat aggcaatctc
     7921 agaattgggg atcatgacac tataatgctc ttcaatgaca ctaaacgcag atttaatagc
     7981 agataaccct tctttttgac actgcttcca tttctctaat ccttgatagg tttcgattgg
     8041 catttgtcgg atcaatcgtt caatgagaca gctcacatgg acatacaacg ctaatttttt
     8101 gggattgctg atgcttcgat tcaactgcac ctctaattct ctcataaaat gatcgatttc
     8161 tcccatcacc ttctctgtat ctagaatagt cacggaatcc agtacttttt ccaatgaaaa
     8221 attacgaatg atgcgctgat tgaatatttc cttctctgaa gcattcatag tagtgtccaa
     8281 ccattcagct agtacagaca tcttttcccc cgagatgagg tcttctaatg aaagatagtc
     8341 gatattggtg atatcaggat ctgtggtgcc aatcattcct acgacttcgt atatggaaaa
     8401 taatgggtca ctgtttttct tatcttttaa ttgctgatat tcatatggga tgattttcaa
     8461 ctgacaagcc gtaggtaagc ttttttctag cagttcactt aagtggaccg ccgtaccgat
     8521 tccagtgaag caagtagtaa tgagtgcttt gggtttgttt tcctctggat agagcagtcg
     8581 ccattctggc tggatctgtc gaacagtctc ttctgctatc tcttctaatg atacattttt
     8641 ttgcaggcat tcccctactg tgattgctaa aggtgtcgtc acattattca aaatcaaaag
     8701 cggtacggat aactgttttt taaaaaactg atggatttct ttcagagatc ccatgtctac
     8761 taatatgatc aatccatttg aaacatcgct gttttcgcaa taatcaagga tttctgaagc
     8821 aatctgctgt ggtgtgacat ctagtggcat atcaaatgat tcaaatagat cctcttgtaa
     8881 taaacgattt actacatttg ccatactgct agcagttgca taaccgtgag cagcaatcat
     8941 cgcttttggc agtcgttttt cttttgacca tttcgctttt tttagataga tcgtcagtaa
     9001 aatccgatcc atacttgtgt cttcaatatc taatttgatc ttacttaatt ccagaattcg
     9061 ttggacatag tgataactag atggataaga ctgagaaact tgttgttcta attctttgat
     9121 tagttggata atatcatgtt cttccggtaa ccatttgata gtgcttcgct gatagagata
     9181 gtaactcaag gcatagacac tgtttccatc gaatttgatt tgataggcag tttccatttg
     9241 tcgaaaggtc tctcggatag attgtgtcca ataaaccagc agctcgtgtt tttcttgtct
     9301 actggtctca aacagcaaaa aatcaaataa ttgaatcact tcttgcttta attttccttc
     9361 gcaagaatat agcagacagt tatttttctt gaaatcaatt aggatttgct caaaactttt
     9421 aacgattcgc tgctgttcgg gctgttgctt ctccattaat tgctctaagg tctggtcttg
     9481 cgtaatatag attccttgct gtgctgtatt tttagtaata ggctgcgcaa gtagttcttt
     9541 ttgcaaatga tggatcgtga catggatttc aggctgttca cgctgttcag catatgcttt
     9601 tgctgctgaa actttgacga tatttttcag ctctcctatg tttccggata atttctgact
     9661 tgttaaaagt gacaaagctg gtccactgac tgacaaaggg ctccccattt ttctttgttc
     9721 ttcgagaaaa aaagagtaaa tcagttctag ccgttcatta cgactacgct gattaagtgc
     9781 aggtagttcg attcggatag gtattcttcg aatgaaagtt gttaagaaat gactagtaat
     9841 ctcttcagtc gtagcaaaaa agagacgtgt tttgatccgg cgtgacaaat taggttctcc
     9901 catacgataa atcacacctt gatcaagata ggtgaacagt ttttcctgac cttctggtgt
     9961 taagcgatgt acctcgtcta aaaacaagat cccaccatct gctgcttcaa aagccccttt
    10021 tttattttct tctgcaccag tgtacgctcc cttaacatgt ccaaacaaat tgctagtcaa
    10081 aagttcagga ttatctgcat attgtgcaca attaaaagtg atgaatggtg cagaatcttc
    10141 tagcagatca tgaagcagac aatactgata gaccaaatta actaaataac ttttacctgt
    10201 gccactttcg cctgtaatca aaagtggtaa tccgccatct ggatagtaaa gagctgtttt
    10261 gatttgttcg atactttctt tcaaactggc gtcgtatccg atcataaaag agaataagtc
    10321 ttttggttgc gcgaaaaaag gctgttcctc tttgagatca tcgatagaaa aataaaaatt
    10381 cgtgcgtaac ttaaaaaatt gtttttcaaa agcttctttg tggaaataaa tcactggtcg
    10441 agaattgatt ttgactagta gttgctcatc atttaactgg ttcaaataat gactgactgt
    10501 gtttcttttt accccaaaat gatcagctaa cctttgtgct gtaaagacct cgttcatatg
    10561 ttctaaatcg aaaaaggctg tctggttctc taagtaacat aaaatttcct tcttcaacaa
    10621 cttattcacc cctcctccat catagaaaac ggattcattt tgtcaataaa atctttaata
    10681 aagaattgaa aaatatatct tattatagac agcagcctac ttatccctta taatttttat
    10741 tctaaaaaac gtacagttcc ttccagtttt tatactggaa tttcaacata tgaaagaata
    10801 aggaggctat gactattcaa tgaaatcgga taaatataaa aacaggaaac atacttcctg
    10861 gaaaactttt cgagaaatca tcggatctta tctcgctgta gttgtatttt tgcctttgct
    10921 ttatatggct atgacagaac aaactcgttt tattggcgta gatacgcaat atatagccga
    10981 aacaggattt ttattcatct ttatcttgat ttttgcgttc tataaatcct tccatacaaa
    11041 ataagattca cagatttcaa ctttcctgtc tgtttttagc tattacaaat ccttatatgt
    11101 ggtataacaa caatttattg gcataatgag gaaaaacgag ttatgatgct ttcagagagt
    11161 aaagaggtac tgttgtcacc atccttttta ttgattacgt acaccactct tagttttttc
    11221 ccattgatta cgaaaacaag gtagaaacat tgatgttcct accttgtttt ctttttttat
    11281 tttcagatca aaatctcaag tggcttttga cttgatgtcg ggaggttgtc ttcgttccat
    11341 caaacctgat agaatctgcg ccaaaccata gttttctcca ctattattta ttcctct
//
LOCUS       DACDOA010000082        11373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDOA010000082 DACDOA010000000
VERSION     DACDOA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.082"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWI39_002073"
     CDS             6..305
                     /locus_tag="IWI39_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209165.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWI39_002074"
     CDS             378..1739
                     /locus_tag="IWI39_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9209166.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWI39_002075"
     CDS             1761..2021
                     /locus_tag="IWI39_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209167.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWI39_002076"
     CDS             2035..2787
                     /locus_tag="IWI39_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9209168.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWI39_002077"
     CDS             complement(2881..3486)
                     /locus_tag="IWI39_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9209169.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWI39_002078"
     CDS             complement(3539..4513)
                     /locus_tag="IWI39_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9209170.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWI39_002079"
     CDS             complement(4891..5670)
                     /locus_tag="IWI39_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9209171.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI39_002080"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI39_002080"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9209172.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWI39_002081"
     CDS             7161..8558
                     /locus_tag="IWI39_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9209173.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWI39_002082"
     CDS             8639..9079
                     /locus_tag="IWI39_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9209174.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWI39_002083"
     CDS             9227..9553
                     /locus_tag="IWI39_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9209175.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWI39_002084"
     CDS             9554..10303
                     /locus_tag="IWI39_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209176.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWI39_002085"
     CDS             complement(10383..10838)
                     /locus_tag="IWI39_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9209177.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3607 a         1976 c         2323 g         3467 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgg
//
LOCUS       DACDOA010000083        11054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDOA010000083 DACDOA010000000
VERSION     DACDOA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.083"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            125..940
                     /gene="thiD"
                     /locus_tag="IWI39_002086"
     CDS             125..940
                     /gene="thiD"
                     /locus_tag="IWI39_002086"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9209178.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1269..2720)
                     /gene="cls"
                     /locus_tag="IWI39_002087"
     CDS             complement(1269..2720)
                     /gene="cls"
                     /locus_tag="IWI39_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9209179.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3061..5727
                     /locus_tag="IWI39_002088"
     CDS             3061..5727
                     /locus_tag="IWI39_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9209180.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDVS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6091..6843
                     /locus_tag="IWI39_002089"
     CDS             6091..6843
                     /locus_tag="IWI39_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9209181.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6865..8019)
                     /locus_tag="IWI39_002090"
     CDS             complement(6865..8019)
                     /locus_tag="IWI39_002090"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9209182.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8204..10645
                     /locus_tag="IWI39_002091"
     CDS             8204..10645
                     /locus_tag="IWI39_002091"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9209183.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10802..>11054)
                     /locus_tag="IWI39_002092"
     CDS             complement(10802..>11054)
                     /locus_tag="IWI39_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209184.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3586 a         1969 c         2379 g         3120 t
ORIGIN      
        1 aaataaattt gattaccaag aaaaggtagt ggtatattta ctgagggata ctttgaaaaa
       61 catatgatag aaacagtaac agtattatga aaaatctttt tataaaaatg gaggaatcga
      121 tttgatggaa gatcagtatg tacaagcaat gacgatcgca ggatcagata gcgatggaag
      181 cgcagggatg caagcagata tgcatacatt ctttacaaga aatgtttacg gtgtttctgt
      241 catgaccgca tgtgttgcag gtaattctta cggaatcgga gcaagtgtga cattgccaac
      301 agattttatc gacaaagaat ttgaactgat tgcgaaggac tttcaaatac gtgcagctaa
      361 aacaggtatg ctagcagatt caaagttgat tgagacagtt gtaaaaaatt accaaaaata
      421 tgattttgga ccattagtcg tggatcccgt aatcgtaacg aaacatggaa atcttttact
      481 agaagaatct gctttgcaaa gcttgaaaga aaagcttgtc cctttggccg aagtactgac
      541 acctaatttt tatgaagcag aaaagctgac tggacggaca ttagcagctg aagaagattt
      601 tgtgcaagca gcaaaagaac tgcaagatat gggtgcaaaa aacgttatga tcaaaggaca
      661 tcatgctaaa aatgcgcagc aagaaacagt caatgactac gtattgttcg aatcaggaaa
      721 atcattttgg ttaaatgcac cattttatcc taccgaccgg atcaacggaa ctggcgattc
      781 gctatctgca tgtattgcag ctgagttagc gaaaggaaaa acagttgaac aagcgatccg
      841 attggcaaaa gactatgtaa accgtgcaat tcgtgaagaa ataaatgtag ggcacaaata
      901 tggccccatc aatcattggg cagctggtga attagagtaa aagaaagaag ttgtgaaaaa
      961 gttgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttaact
     1021 tggttggact tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga
     1081 gcctgccgtc cagctccgag tcacaaggaa caagttcaaa aaacagcttc tcatgtttgt
     1141 tgaatattgt gacttgtgac cgaggagttg gcaggagaac accgtttgtt cggaagaagg
     1201 gactgtgaca agatttttgt cacagtccct ttaatcttag tatatttttc tctacttgaa
     1261 taaaaaaatt ataggattgg agaaaataat cgagaaattt gttgcttgaa taatagccag
     1321 ttagacattt ccttgactgt ttcagttgtc atggatgaac aatgtgtctc atcttctttg
     1381 aactgagtag caagttcttc taaaaactca ggatcgtaaa tgacagcact tgtttcaaaa
     1441 ttcaatcggt agctacgaat atcttgattt gctgaaccta ccatgcagat ttcatcatcc
     1501 attatcaatg tttttgcatg gaggaagccg ttctcataga caaggatttg tacattttcc
     1561 ttgatcagct gccgagcata atattgtgtt gctcgataaa taaaaggatg atccggtttg
     1621 ttgggaatca tgattttcac atctacacca gaggcagttg cgatttttaa agcagcaatg
     1681 acactttcat caggaacaag gtaaggtgtc tgtatccaaa cacgtttttt agcagaagta
     1741 atcaatttga taaatgaaag cttgatttgt tccctttcgt tattcggtcc gcttgaaaca
     1801 agctggatgg atgtatcagc aagcttagat tcatctcgat ctgctttttt aaagaaataa
     1861 ttcaaatgat agccgacctt tttttcttca ggtaccgaga cgttccagtc tgttaagaag
     1921 cgaagctgga gcaaagaaga agcggcaccg aaaatccgta tatgcgtatc gcgccaatag
     1981 ccgaattttt ttgttgtatt tacatattga ttggcaatat tgaaaccacc ggtataacta
     2041 atctttccat cgatcacaac gatttttcgg tgatcatggt aattcaatcg gaaacgaagt
     2101 aatgcccttt gtgaggtaac aaatgtatgg acatgtccac catttttgat tagacgattg
     2161 aaatcttttg cttttgtgcc atgagagcca aatgcatcat ataatattcg aacttccacg
     2221 ccttctgcgg ctttttcttc taaaacatgt aagacttgct ggccgatatt atctgtcaca
     2281 aatgcgtaat attcgatatg gatcgaatgt ttggcttttt ttagatcttg aagcagtgaa
     2341 tccaatttct cttgtccgtc tgtgagaaga gtgacagaat tcattcttgt cagcggcata
     2401 cgatttaatg aagagaagaa gtcaacaaat tgctgtttgt ctttatcgcc catgtcagga
     2461 tcgtaatgtt caatggtatc tcctctaaaa gactgaaagt tttctaattc tttcaagtca
     2521 ctttgtcgga gatagaattt tttcttatcc gttaatccac gtccgaaaaa taaatataat
     2581 acaaagccca ccccaggaag ggcaaacaat actaagagcc atgcccaaat ggctgctaca
     2641 tctcggggtt tgatcaaaat agccaccaaa gcaataagtg catttaatag ataaagggcg
     2701 gttataatac tagataccac aaaaatgttc ctccttacat acaataacat tagtatagca
     2761 tgatagaaga aagaagccta ccaaggtgaa tatacttttc actcctttca tgaaaatgta
     2821 ggtaaatcgg gctaaagtag ttgctttttg taggttaata aggtaggata ttagtacagt
     2881 tgaatatctc cttaggggag taaccggcaa cgtttgttgc gataatatca acagtaagtt
     2941 aaccaaatgg ttttctggta ttgtatgaaa tggtgagacc taagacaaat gcgtatttgt
     3001 cttaggtctc ttttttttca gacaggacta gggccaagat caaaaaatgg gggatgaaat
     3061 ttggaaaaca aggaacaagc acaacaagcg gtctataaag aaacgatcga ctcgttgatg
     3121 cagcaaacgc agagctcaac cgatggattg agcaccacgc aagcgaagga aaaattagaa
     3181 agagatggct tgaaccagct aaaagaaaag ccgaagaagc caacttggaa attgttctta
     3241 gaaactttca aagatgcaat ggttatcgta ttgctgattg tcgcactgat tcagatggtc
     3301 atgggtgact acatcgaatt cttagttatt tttgcagtat tgatgttgaa ttctataatc
     3361 agtgttgtcc aaacaaaaaa agcagaaggt tctttagaag cgttgaaaaa cctttcagcg
     3421 cccgatgctc gagtgattcg tgatggtaag gaaaaaacga ttccggcaaa cgaacttgtg
     3481 gtaggagata tcgtgttgtt agacgcaggc gattatgtac ctgctgatgg acgtttacta
     3541 gatgcgggat cattaaaagt agatgaaggt atgctgacag gagagtcgac ttctgcagaa
     3601 aaagaagtaa cggatatcac aagtacggta ccaatcggag atcgtacaaa tatggtattc
     3661 agtggaacga tcgtaactta cggacgcggt aaattcttag tgacttcaac tgggaataat
     3721 acggagatcg gacaagttgc tggccttttg gaatcaacaa ctgagcaagt cactccgtta
     3781 caacgaggtc tggataaatt cagtaaaaaa ctaagtttgg ctattttggc attgtcttta
     3841 ttgattttag gtatccaact gtttcgtatt tatcttggag aaggtacagg agatatgact
     3901 gcagatatcg tcagcgcagt gatgtttgcc gtagccgtag ccgtagctgc tattccagaa
     3961 gcattacagt ctatcgtaac aatcgtgtta tctttgggaa cgaataaaat ggcaaaacgt
     4021 catgccatta ttcggaagct tccagcagtt gaaacacttg gttcgacaag tatcatctgt
     4081 acagataaga ctggtacatt gacacaaaac aaaatgacag ttgttgacta ctttttaggt
     4141 aatggcgata ctggggattt cacaaatgac ccttcaaaat ggacagctga cgaacgtcgc
     4201 ttgatcgaaa tcagtgtttt agcgaatgat gtatcgatca gtgaagacgg tactaaattg
     4261 ggcgacccta cggaagtagc gtttatcgac tttagtgaaa aattaaacca gccttatcag
     4321 gaaatccgaa acaactaccc aagacaagca gaacttcctt ttgactcaga tcgaaaatta
     4381 atgtctactg gacacacaat tgataacaaa aattacttgt ttgttaaagg cggaccagat
     4441 atcgtcttcc aacgcagtaa aaaagtattg ctaaatggtg aagtcgttcc gatgacggat
     4501 gaattgatga aaaaattcca agagcaaaat gaagcttttt caacacgcgc attacgtgta
     4561 ctagcttttg cttaccgtcc acttgataat tttgatttat catttgaaga tgaagacgat
     4621 ttgatccttg ttggtttgct tgctatgatc gatcctccac gtgctgaagt gactcaggcg
     4681 gtaacagaag caagaagtgc aggaatcaaa acaatcatga ttactggtga ccacaaaaca
     4741 acagctgttg ctatcgcaaa acaaatcggg atttttgaag aaggaaacct ggctttgacc
     4801 gggacggaac tagatgaatt gacagaagat caactaaacg aagttttaga aaaagtcact
     4861 gtttatgcac gtgtttcacc tgagaataaa attcgtatcg ttcgtgcttg gcaaaataaa
     4921 ggtcatatct cagctatgac aggtgatggt gtaaatgacg caccagcatt gaaacaagca
     4981 gacatcggaa ttgctatggg aacgggtaca gatgtggcaa aagatgcagc agcgatggta
     5041 ttgacggatg ataactttgc ttctattgtc agcgcagtag aagtaggacg taatgtgttt
     5101 gataatatca agaaagctat ttcttatcta tttgcaggaa atttaggtgc gatcattgcg
     5161 atcatctttg ccttagttgc tgactggtcc aatccattta cagcattaca actgttgttc
     5221 atcaatttag tcaatgattc actgccagca attgcattag gtacagaaaa agctgaacct
     5281 gctacgatga accgcccgcc aagagatcca aattcaggaa tctttacgga caaaacattg
     5341 atttctgtaa cgtatcgtgg tgtgctgatt gggattgctg ttatctttgc tcaatggctt
     5401 ggcatgcagc attcacctga attcggtgta gccatgtcat tttcaacatt gatttggtgt
     5461 cggacattgc aaacattccc agcacgttca aacacacaaa cagctatcca agcagggttc
     5521 ttctctaaca gaagtgtctt actagctgta gttgtatgta gcgcgcttta ctgcttaaca
     5581 atgatcccag gactgaaaga agtcttctct atcccatcag catttggatt gacagaagtc
     5641 tttacatgta tgggaatcgc acttgtgaca attgttttga tggaaatcac taaattgatc
     5701 attgtacgag tacaaggaaa taaataaaac gaatcaacga ggttgtgagc ctgccgttta
     5761 attctgagtc acaaggtgca atttcaacaa acagcttctc atgtttgttg aatattgtga
     5821 cttgtgaccg aagagttggc aggagaacac cgtttaagga agaagttgta aaatcagcat
     5881 tttgagctaa tactgagggt catctgattg gacactgttt attcggaaag agggactttg
     5941 acacgacttt ggtcacagtc ccttttttta tttatgatag acggaaagcc aatcatttta
     6001 ttattcatca aaagagatag aatagataaa agaggagaat gatttattcc gaaaagtgaa
     6061 aacatataac aaaaaattag aggaaatcaa atgaatgaaa aagaatggga tgaatttgct
     6121 gaagaatatt atgaaatcca gcaagaatcg caagtaacta ttgctgaaga tgtaaaaaat
     6181 tatctgaaaa cggagaaagt cctgcctgct gaaacagcag cagacgttgc tggaggatct
     6241 ggcagatttc tttccttagc aaaagaaatt aatcgctatg atttgatcga tttttcttca
     6301 ggaatgctag aaaatgcaaa aaaagaagca gaaaaaaatg agttgccaaa tgtttccttt
     6361 atcaaacagt cgttagaaga atttatccaa tcaaatgaga catatgaaat ggtttttaca
     6421 gcagcaaatc cagcattggt acatcttatt caattagaaa aattgatgaa aaagtcaagc
     6481 aaatggtgcg tgataatcag agtagtggaa tcaatcgatg atgtttttta tcctatcgaa
     6541 aagcaactag gaatgataga agaagatcct aatacatcgc cagttgtaat ggatcaattt
     6601 gaaacatact taattgatga aggattcaaa gtgaagaaaa aagattttac ttattttaca
     6661 caggaatcaa ttactgaaga attgatgaag ctatactatg acgcgtacgc aaaagatcct
     6721 gttgtgacca ggtacattcg caaactattc agtgaaaaac gagaaatcct tagtacgact
     6781 aagctgacct atagactgct cctttggtcc gcagataaaa aaagaacctt tcacgactcg
     6841 tgaagggttc tttttatgat tttattatat tttgtagtaa cgaatatcat tttcgatctt
     6901 tgtaatcgaa aaaggtacat ctgaagtata ttctgcacat tctccgtgtt caggaagcgt
     6961 ctcactgaaa atcgtttcat attcgtcaac agtgatccgt gttcgagaat ctagcatctc
     7021 ttgatgagat tgtgcgaaga gatattcttg atagttttct tccagataac ctgtaaaaaa
     7081 ttcacttact gcaccagaac cgtagctgaa tagaccgatg cgatctcctg gctgtagtga
     7141 tttcgaattt tccagtagag aagttagccc caggtataat gaaccagtgt aaagattacc
     7201 gattcgtcgg ctgtaacgga tgctttcttc atagcgagcc ataagacgtt cctgattttc
     7261 ttcgtctgtt tggtctaata cgctttgcaa tgcctttttt cccatcttag tatacggaat
     7321 atggaaagca atcgcctgat aatcttcgag tcctcgaccc gacaattctt tatgtcgatt
     7381 ccaaactttt tggaatgatt cgatatacgt agaattagat aaaggaccat caacaacagg
     7441 aaattcgcta taatctggac gccagaaatc atagatatct tctgtcagaa atacgctgtc
     7501 gtcttcaatc gataaaatac gcgggttttg agtgatcatc atcgcaacag caccgacacc
     7561 ttgcgtcact tcaccaccgc ttgccaagct gtaacgagca atatcacttg ctatgactag
     7621 tacttttcgt tctggatgat ttttgacata ttctttcgcc atgtgcaaag ctgcggtgcc
     7681 accgtagcaa gcctctttta cttcaaaaga acgagcaaaa gattggattt tcagtaaatg
     7741 gtggataatt accgcactcg cttttgaatg atcaatcccg gattctgttc cgacgatgac
     7801 catgtcgatt agctcacggt ctttttctgt tacgattttg ctggcagcat ttgctcctag
     7861 tgtaatgatg tcttcatttg cacgattgac tgccatttgg tcttgaccaa tccctatatg
     7921 gtattttgca ggatcatccc cacggctttc tgccaactct gtcatatcta aatataaatt
     7981 aggaataaaa aaggaaagac gatcaatccc tattttcatt tcaacacctc tttacttatg
     8041 ataaaaaact tactttacta aggtatcata aaaagattaa aagaaatata aaaaagagaa
     8101 tatttaagga ttgttaaaat ttaatcttag aaaaaaataa ctggaacaag agctatttgt
     8161 ttttacagca aagaatggta tatttttgta ggaggtggct tacttgaaag aagtcgttat
     8221 gatagatgct gcaagaacgc cgataggaaa gtatcgcgga agtttaagtc catttactgc
     8281 agttgaatta ggcacacttg tgactaaagg attattagat aaaacaaaac tgaaaaaaga
     8341 taaaatcgat caagtaatat ttggaaatgt cctgcaagct ggtaatggtc aaaatgttgc
     8401 cagacagatt gctttgaaca gcggtctccc tgtagatgtc ccagcaatga cgatcaatga
     8461 agtttgcgga tctggtatga aagcagtgat cttagcacgc cagctgatcc aattaggtga
     8521 agcagaactt gtgatagccg gcggaacaga gagtatgtct caagcaccta tgctgaaaca
     8581 gtatcagtca gaaacaaatg aatatggtga accaatttcc agtatggtca acgacggatt
     8641 gactgacgca ttttcaaatg cacatatggg attaaccgca gagaatgttg caacacaatt
     8701 ttctgtgagc agagaagagc aggatcgcta tgccttgtcg tcccagttga aagcagcaca
     8761 tgctgtcgaa gccggtgtat tttctgagga gatcatccca gtcaagattt ctgatgaaga
     8821 cgtgttatct gaggatgaag cagttcgtgg aaatagtaca ttggaaaaac tgggcacgtt
     8881 acgtacagta ttctcagaag aaggaactgt aacagcagga aatgcttccc cgttgaatga
     8941 cggtgcctct gtggtgatcc ttgcatccaa agaatacgca gaaaataata atctgcctta
     9001 tttagcaacc atcaaagaag tagcggaggt cggtattgac ccaagtatca tgggaatcgc
     9061 tccaatcaaa gccattcaaa aattgacaga tagatcagga atgaatttat caacaatcga
     9121 tctttttgag atcaatgaag catttgcagc ttcttctatc gtagttagcc aagaactgca
     9181 gttagatgaa gaaaaggtca atatatatgg aggagcaatc gcattaggcc atccaattgg
     9241 tgcaagcggt gcacgtatct tgactacatt agcttacgga ttgcttcgtg aacagaagag
     9301 atacggtatt gcttcattat gtattggtgg aggtttaggc ttggcagtct tgttagaagc
     9361 gaatatggag caaacacata aggatgtgca aaaaaaaaag ttttatcagc ttacgccatc
     9421 tgaacggcgc agtcaattaa tagaaaaaaa tgtattgaca caagaaactg cgttgatttt
     9481 tcaggaacaa acactttctg aagagctctc cgatcacatg atcgaaaacc aagtgagtga
     9541 agtggaaatc ccaatgggga tcgcgcagaa ttttcaaatc aatggcaaga aaaaatggat
     9601 tccgatggca acagaggaac catctgtcat tgcagctgca agtaatggtg caaaaatctg
     9661 tggaaatatc tgcgcagaaa cacctcagcg gctgatgcga gggcaaatcg ttctatcagg
     9721 aaaatcggag tatcaagcag tgatcaatgc agttaatcat cgaaaagaag agctgattct
     9781 ttgtgcaaat gaaagttatc cttcaatcgt gaaacgcggg ggcggtgttc aagatatctc
     9841 tactcgggaa ttcatgggga gtttccatgc atacttatct attgattttt tagtggatgt
     9901 aaaagatgca atgggcgcta acatgatcaa ctcgattctt gagagtgtgg caaacaagtt
     9961 acgggagtgg ttccctgaag aagagattct attcagtatt ttaagtaatt ttgctacaga
    10021 atcattggct tctgcctgtt gtgaaatccc attcgaacgc ttagggagaa acaaagaaat
    10081 cggtgaacaa attgccaaaa aaatccaaca ggcaggggaa tatgcgaagc ttgaccctta
    10141 ccgtgcagca acgcacaaca aagggatcat gaatggtata gaagctgtag tggcagctac
    10201 aggaaatgat acacgtgcgg ttagcgcttc gatccacgcc tacgcggcaa gaaatggatt
    10261 ataccaagga ttaacagact ggcagatcaa aggggataaa ctagttggaa aactaacggt
    10321 gcctttggct gttgccacag taggcggagc ttccaatatc ctgccaaaag caaaagcatc
    10381 tttagctatg ttagatattg attcagccaa ggaactagca caagtaattg ctgcagtagg
    10441 gttagctcaa aacctggctg ctttgcgtgc attagttaca gaaggaatcc aaaaaggcca
    10501 catgggactt caagctcgtt cattagcgat ctcaattggg gctattggtg aggaaatcga
    10561 gcaagttgcg aaaaaattac gtgaagcaga aaagatgaac cagcagacag ctatacaaat
    10621 attggaaaaa atccgggaaa aataaaaaag caatatcttt agaagaataa agcaagattt
    10681 acagttattt tatttactgc agatcttgct tttttggctc ttcgtcaaat tgtgttggcg
    10741 aagacaaatt tgaataaaac ttagtccgat atgcggcgcg ttctgtgcgc cgcatatttt
    10801 tttagttttc aaagatcaga ccttgttgta aatagatccg tgcccgaaag ttataaaagt
    10861 tgcgataacc aaacgcaatt cgtttaatca gcttgatctt gttattcagc ccctcagtga
    10921 cgccgtttga gtggggcacc ttaaaggcat ttgaaatacc cgattggtag cgatggaaga
    10981 tcgtgaattt ctttttaaac gactgaggta acgtagatga aattgtttta agggtttctt
    11041 gaaagccttc cgta
//
LOCUS       DACDOA010000084        10990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDOA010000084 DACDOA010000000
VERSION     DACDOA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.084"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IWI39_002093"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IWI39_002093"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            188..260
                     /locus_tag="IWI39_002094"
     tRNA            188..260
                     /locus_tag="IWI39_002094"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Val,seq:tac)
     gene            264..336
                     /locus_tag="IWI39_002095"
     tRNA            264..336
                     /locus_tag="IWI39_002095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Lys,seq:ttt)
     gene            344..425
                     /locus_tag="IWI39_002096"
     tRNA            344..425
                     /locus_tag="IWI39_002096"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:378..380,aa:Leu,seq:tag)
     gene            443..515
                     /locus_tag="IWI39_002097"
     tRNA            443..515
                     /locus_tag="IWI39_002097"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Thr,seq:tgt)
     gene            527..598
                     /locus_tag="IWI39_002098"
     tRNA            527..598
                     /locus_tag="IWI39_002098"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:559..561,aa:Gly,seq:gcc)
     gene            615..700
                     /locus_tag="IWI39_002099"
     tRNA            615..700
                     /locus_tag="IWI39_002099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:649..651,aa:Leu,seq:taa)
     gene            711..784
                     /locus_tag="IWI39_002100"
     tRNA            711..784
                     /locus_tag="IWI39_002100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Arg,seq:acg)
     gene            794..867
                     /locus_tag="IWI39_002101"
     tRNA            794..867
                     /locus_tag="IWI39_002101"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:828..830,aa:Pro,seq:tgg)
     gene            887..959
                     /locus_tag="IWI39_002102"
     tRNA            887..959
                     /locus_tag="IWI39_002102"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Ala,seq:tgc)
     gene            987..1060
                     /locus_tag="IWI39_002103"
     tRNA            987..1060
                     /locus_tag="IWI39_002103"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Met,seq:cat)
     gene            1074..1147
                     /locus_tag="IWI39_002104"
     tRNA            1074..1147
                     /locus_tag="IWI39_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1108..1110,aa:Ile,seq:cat)
     gene            1171..1260
                     /locus_tag="IWI39_002105"
     tRNA            1171..1260
                     /locus_tag="IWI39_002105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1207..1209,aa:Ser,seq:tga)
     gene            1273..1346
                     /locus_tag="IWI39_002106"
     tRNA            1273..1346
                     /locus_tag="IWI39_002106"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1307..1309,aa:Met,seq:cat)
     gene            1365..1440
                     /locus_tag="IWI39_002107"
     tRNA            1365..1440
                     /locus_tag="IWI39_002107"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1398..1400,aa:Asp,seq:gtc)
     gene            1442..1512
                     /locus_tag="IWI39_002108"
     tRNA            1442..1512
                     /locus_tag="IWI39_002108"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1474..1476,aa:Gly,seq:tcc)
     gene            1526..1599
                     /locus_tag="IWI39_002109"
     tRNA            1526..1599
                     /locus_tag="IWI39_002109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1560..1562,aa:Ile,seq:gat)
     gene            1624..1711
                     /locus_tag="IWI39_002110"
     tRNA            1624..1711
                     /locus_tag="IWI39_002110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1658..1660,aa:Ser,seq:gct)
     gene            1733..1804
                     /locus_tag="IWI39_002111"
     tRNA            1733..1804
                     /locus_tag="IWI39_002111"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Glu,seq:ttc)
     gene            1827..1916
                     /locus_tag="IWI39_002112"
     tRNA            1827..1916
                     /locus_tag="IWI39_002112"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Ser,seq:tga)
     gene            1929..2002
                     /locus_tag="IWI39_002113"
     tRNA            1929..2002
                     /locus_tag="IWI39_002113"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1963..1965,aa:Met,seq:cat)
     gene            2021..2093
                     /locus_tag="IWI39_002114"
     tRNA            2021..2093
                     /locus_tag="IWI39_002114"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2054..2056,aa:Asp,seq:gtc)
     gene            2097..2169
                     /locus_tag="IWI39_002115"
     tRNA            2097..2169
                     /locus_tag="IWI39_002115"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2130..2132,aa:Phe,seq:gaa)
     gene            2174..2244
                     /locus_tag="IWI39_002116"
     tRNA            2174..2244
                     /locus_tag="IWI39_002116"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2206..2208,aa:Gly,seq:tcc)
     gene            2257..2330
                     /locus_tag="IWI39_002117"
     tRNA            2257..2330
                     /locus_tag="IWI39_002117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2291..2293,aa:Ile,seq:gat)
     gene            2342..2413
                     /locus_tag="IWI39_002118"
     tRNA            2342..2413
                     /locus_tag="IWI39_002118"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2375..2377,aa:Glu,seq:ttc)
     gene            2679..3872
                     /locus_tag="IWI39_002119"
     CDS             2679..3872
                     /locus_tag="IWI39_002119"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9209185.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4002..5474
                     /locus_tag="IWI39_002120"
     CDS             4002..5474
                     /locus_tag="IWI39_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9209186.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5557..6345
                     /locus_tag="IWI39_002121"
     CDS             5557..6345
                     /locus_tag="IWI39_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9209187.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6412..7080)
                     /locus_tag="IWI39_002122"
     CDS             complement(6412..7080)
                     /locus_tag="IWI39_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9209188.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7195..8907
                     /locus_tag="IWI39_002123"
     CDS             7195..8907
                     /locus_tag="IWI39_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209189.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8910..10682
                     /locus_tag="IWI39_002124"
     CDS             8910..10682
                     /locus_tag="IWI39_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209190.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10764..>10990)
                     /locus_tag="IWI39_002125"
     CDS             complement(10764..>10990)
                     /locus_tag="IWI39_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209191.1"
                     /translation="SLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGH
                     EDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3153 a         1992 c         2538 g         3307 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcttaagt
      181 tatccatgga ggtttagctc agctgggaga gcatctgcct tacaagcaga gggtcagcgg
      241 ttcgatcccg ttaacctcca tatgagccgt tagctcagtt ggtagagcat ctgactttta
      301 atcagagggt cacaggttcg agccctgtac ggctcatatt tttgcgggtg tggcggaatt
      361 ggcagacgca ctagatttag gatctagcgc cttacggcgt gggggttcaa gtcccttcac
      421 ccgcataatt taagttttac atgccggctt agctcagttg gtagagcatc tgatttgtaa
      481 tcagagggtc gagggttcaa gtcctttagc cggcatcttt aaacacgcgg aaatagctca
      541 gtggtagagc accaccttgc caaggtgggg gtcgcgggtt cgaaccccgt tttccgcttt
      601 gccaaagcaa tcgcgccggg gtggcggaac tggcagacgc acaggactta aaatcctgcg
      661 gtgagtgatc accgtaccgg ttcgattccg gtcctcggca tttcaaatat gcgcccatag
      721 ctcaactgga tagagtgttt gactacggat caaaaggtta ggggttcgac tcctcttggg
      781 cgcgttaatt aaacgggaag tagctcagct tggtagagca cttggtttgg gaccaagggg
      841 tcgcaggttc gaatcctgtc ttcccgataa ttataaattt aaacacgggg ccttagctca
      901 gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc taggctccat
      961 ttcatttaaa taatgaatat ctttttcgcg gtgtagctca gctggctaga gcgtccggtt
     1021 catacccggg aggtcggggg ttcgatcccc tccgccgcga tttttaagag cctggacctt
     1081 tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     1141 aggtccattg tggcgttgta agtttcatat ggaggattac ccaagtccgg ctgaagggaa
     1201 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1261 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1321 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cactggttcc gtggtgtagg
     1381 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgcca
     1441 tgcgggtgta gtttagtggt aaaaccacag ccttccaagc tgttgtcgcg agttcgattc
     1501 tcgtcacccg cttttagttt tttatgggcc tatagctcag ctggttagag cgcacgcctg
     1561 ataagcgtga ggtcgatggt tcgagtccat ttaggcccat tagtacaaaa tcttattggt
     1621 tttggggaag tactcaagtg gctgaagagg cgcccctgct aagggtgtag gtcgggaaac
     1681 cggcgcgagg gttcaaatcc ctccttctcc gtttcaatat tttgttttac ctggcccgtt
     1741 ggtcaagcgg ttaagacacc gccctttcac ggcggtaaca cgggttcgaa tcccgtacgg
     1801 gtcattaatt taatatagtt ttacatggag gattacccaa gtccggctga agggaacggt
     1861 cttgaaaacc gtcaggcggg taaaaccgtg caagggttcg aatcccttat cctccttttt
     1921 catattatcg cgggatggag cagtctggta gctcgtcggg ctcataaccc gaaggtcgta
     1981 ggttcaaatc ctgctcccgc aattaaaaac taaaaacatt ggttccgtgg tgtaggggtt
     2041 aacatgcctg cctgtcacgc aggagatcgc gggttcgatt cccgtcggaa ccgtttggct
     2101 cggtagctca gttggtagag caatggattg aagctccatg tgtcggcagt tcgattctgt
     2161 cccgcgccat tatgcgggtg tagtttagtg gtaaaaccac agccttccaa gctgttgtcg
     2221 cgagttcgat tctcgtcacc cgcttttagt ttttatgggc ctatagctca gctggttaga
     2281 gcgcacgcct gataagcgtg aggtcgatgg ttcgagtcca tttaggccca tttattttat
     2341 tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac gggttcgaat
     2401 cccgtacggg tcatggaaga tactttttga gtatcttttt ctttttatct gaaagaatga
     2461 ttttaaaaag cttagcagag ttatctttat ggatgattgt gctaagcttt tttggacagt
     2521 tctaatgtaa aatattttta tattttttaa gcaaaaaaat gaaaaaatac gagcagaaaa
     2581 ctgttttatc tgctatactt ggttcgttgc aagttcccga aaggatccgc aggacggaga
     2641 tgccttgtaa ccgaatttaa ctagggggaa tcattataat ggtagaaaga cacttattta
     2701 catcagaatc tgtttctgaa ggacatccag ataaaattgc tgaccaaatc agtgatgcaa
     2761 tcttggatgc aattttaaaa caagatccaa cagcacgagt agcgtgtgaa acgtctgtaa
     2821 caactggtct tgttttagta tttggagaaa tatctacgac ggcatatgta gatattcaaa
     2881 aagtcgtacg ggaaacaata aaagaaattg gttatacacg tgcaaaattc ggatttgatg
     2941 gagatacagc agctgtattg gttgcgattg atgaacaatc tcctgatatt gctcaaggag
     3001 ttgatgaagc acttgagatc cgggatgaag ataaaaaaga cgtattagat gagattggtg
     3061 ctggtgacca aggtttaatg tttgggtttg ctgtagatga aacaccagaa ttgatgcctt
     3121 taccaatcgc tttgagtcat cgtctggtac gacgcttggc agatttgcgt aaatcaaatg
     3181 aattaacgta tttacgtcca gatgcaaaat ctcaagtaac ggttgaatat gatgatcaag
     3241 gacaaccgga acgcgtagat acaatcgtta tttcaacaca gcatgatgat gcagtagata
     3301 atgaaacaat tcgtcatgat gtcatcgaaa aagtagtgaa ggaagttatt ccagctgaat
     3361 tattagatga tcaaacgaaa tattatatca atccgactgg ccgatttgtc attggtggtc
     3421 ctcaagggga tgccggatta acaggaagaa aaatcattgt tgatacgtat ggcggttatg
     3481 ctcgtcatgg tggcggtgct ttttctggta aagatgcgac aaaagttgac cgttctgcca
     3541 gctatgctgc acgttatatt gctaaaaaca ttgttgcagc agggcttgct cgcaaagcag
     3601 aagtacagct agcttatgcg attggtgttg ctcaacctgt ttcgatctca atcaatactt
     3661 tcggtactgg aacggttccg gaagaagaac tgattgctgc agtgagagaa aacttcgatc
     3721 ttcggccagc aggaatcatc gagatgcttg atttgcgtcg tccgatttac aaacaaacag
     3781 ctgcttacgg tcattttggt cgtacagatg tagatttgcc ttgggaacat acggacaaag
     3841 tagatgcgtt gaaagcaagt ttagcaaaat aagttatatt cagctggaac gatcgccaag
     3901 tggtggttgt tccagctttt tttatggcag ttaacctctt gaaaaagata gtaattattt
     3961 atctattttt agatcctgtc ataatgaaag ggaggaataa gatgaaaaaa gaaacaaatg
     4021 ttactttggt cacagtgagt attttcattg caacgttcat gacggctatt gaaggtacaa
     4081 tcgtaacaac cgctatgcca acgattgtcg gttcattaca tggaatggaa atcatgaact
     4141 gggtattttc gatttatttg ctgacaaacg ctatgcttac tccgatttac ggaaaacttg
     4201 cggataaagt gggacggaaa ccaatcttta tttttgggac attattgttt attatcggtt
     4261 cgttattttg cggtttatct caaagcatga ttgggttgat tgctgctcgt gcgattcaag
     4321 ggatgggtgc tggggcaatg atgccagtag ctttgacgat catcggcgat ctttattcta
     4381 ttgaaaagcg agcaaaagtt ttaggtttga atagttcagc ctgggggatt gccagtgttt
     4441 ttggaccgct tgcgggagga ttcatcgttg aaacgatcag ctggcactgg atctttttta
     4501 tcaatgtgcc gatcggaatt ctattaatta ttttgatttg gctatattta aatgaaccaa
     4561 aaatcataca tgagtctaaa ccaatggata tttttggtag ttttagtttg atggccatgc
     4621 ttgtctcatt gctgttaggg ttccaattat taggagacga aggaatgagt ccgcttgttt
     4681 ctgctttgct gctaggtagt atcgtaatgt taggtatttt tgtttttgcc gaaaaaagag
     4741 ctaaagatcc tgtcatcatg atgcatttat tcaaaaatcc actatttgtt gtggtcaact
     4801 tggctgcagc attggctagt ggttttttga tgggaatcga tgtttatatc cctatgtgga
     4861 tgcaaggtgt attagggctg aatgcagcaa ttggtgggct ggtattagca ccattatcac
     4921 tagtctggat ggtcggttct ttccttgcaa gcaagtggat gactcaaatg aaaactcatt
     4981 gggtcttgag aattggacta ctaatcgcag ctattggggg cgcttggttg tatcttatgc
     5041 ctttcagaag taactattta tggttttttg ctatatcaac ggtcatcggt ataggcttag
     5101 gtgtgaccat gactacctcg actgtcagtg cacaaaatag tgtatctcgt gaccagcttg
     5161 gtgtagcaac ttcttttaat acgttagttc gaacaattgg tcaaacagtc atggtttcta
     5221 tttttggttt attgataaat ggggtaacta aacgagaact tgcagcagca aatttaacaa
     5281 atgatgcaga tatcatgaat aaactggtga atccacaaac ggctaaatat attgatgcgg
     5341 atattttgtt accattaaga aaaattcttt atgatgggtt acattccgtt tatctagttg
     5401 gcttgttact cgtgatcatt gctttggtct taacgctttt cgttcatgaa aaaagtggta
     5461 aaataaaaca gtagtttcgg ttaagatatg tacgtagccg aatcattcaa atttaaagtt
     5521 attcaaaaat aacttcttac taaggaaggc gaaaatatgg catttattca agcaaatatt
     5581 tattcgaatg tgttagaaat ggaagtcaat ctaaatgtga tactaccgca aacaacaaaa
     5641 aaggtgattg gcaccagttc agagcaagag acaacggata tacctgtttt atatttactt
     5701 catggaatgg gtggcaatca tagtgtatgg gaaagacgta cgtcgattga acgttatgta
     5761 gcaaactacg gattagctgt catcatgcca tctactgatc ttggctggta cacagataca
     5821 gtttatgaca tgaagtattg gacattcatt gcagaagagc tacctgagat ttgtcatgaa
     5881 ttattcccgc aattaacaag aaaaagagaa aaaacatatg ccgctggatt atcgatgggc
     5941 ggatatggcg cgttaaaact aggccttgca aaatcagaaa atttcgcagc agtaatctct
     6001 ctttcaggcg ctgtttctgt aggaaatcga atggatgatc ttttgatggt tcgtaaagca
     6061 tctttttggg aagggatctt tggaccattg gatcaaatcg aaggttcaaa aaatgatccg
     6121 caatacttat tgaaagaact agtaaaaagc ggaaaaccag ttccaagatt ttatatggct
     6181 tgtggggaat ctgattttct ttatcaggca aatcaggaaa tggtatctgc tatgcaggaa
     6241 caaggcttgg cggtcacctt tgaacaagga cctggcgatc ataactggat cttctgggac
     6301 caatggattc aacgtgcatt ggcgtggcta atggaagaaa aataaaaaaa gagtcttaaa
     6361 aagatagctc ctatagagat cacaaacata gtgatctcta taggagcttt tttactgctc
     6421 gttttctctt cttttatttt gttggcataa gccttcaata taggtttgtg cactcgttag
     6481 aaagagtgct ttttcattca attctgcatg ggactgcata ctttctttaa atccctctaa
     6541 ttgattttca actacataat tacgcataaa tagaactgtt tctttcaaaa attcatttcc
     6601 gtccataatt ttttgtcgaa gctgctgttc atgctgctga tccattaact gtccaatcaa
     6661 taaattgtgg agagaggcaa tggcctgatg actttgggtg actgaaaatc cttcacgaac
     6721 aagtaattgg atcatttgct ccaaatgttc taatcgactc ggaaaagcgg gcacagtatt
     6781 gatttctaat tctgccccac aagggaaacg tgtataaagg tcatagtaat tttccatata
     6841 ctgcagcaac tgttcattcc aaggcaaagt tggatctgga agcgctaatt cttcttccat
     6901 tgtttcagcc attttttgca agagctgttg tttgtttttg aaataccaat ataaagcagg
     6961 tgcttgaaca tgaattctgt ttgcgatgtt tcgcatggaa agctgcgaaa gagcttgtgt
     7021 ttcagccagg atatcaaagg cggcctgaat gatgattgct tgagaaagct ttatttccat
     7081 tttatcaacc tcgtgtattg cattatcttt ttaaacatgg taaattgtta gtcggtctaa
     7141 ttttacagtg ttaaattagt cctgtcaatt taaacaaagg ggtgtaatca acgaatgaaa
     7201 ttaatcttac gacatgcgaa aaaatataaa gtatctgttt ttatttcctt gctgtccgta
     7261 gcagtcatgg ttgcagcagc attatggcaa cctaagttac tacagcaagt attggaagct
     7321 attattacag aagataatga tgaaatgaaa acaattggta tttacctgat cagtctggct
     7381 ttgctaggat tagccgcagg tgtaacgaac acgattttct ctgcaaaagt agctcaaggc
     7441 gtaagtgcag atatccgaga agaagcattt cgaaagatcc agacattttc atttggaaat
     7501 atcgaacagt tctctgctgg aaatcttgtt gttcgtctaa caaatgatat cacgcaaatc
     7561 caaaatttag tgatgatttc tttgcaatca ctttttcgaa ttccattctt atttattggt
     7621 gccttcattt tagctatgat cacgatgccg caattatggt ggatcatcgt agcgcttatc
     7681 gttacggtat ttctgattac ggcactttca tttacaagga tgggcaagca cttcatgatc
     7741 attcaaaaac tgattgatcg tgtgaatagc attgcaaaag aaaacttact agggattcgt
     7801 gtagtgaaat cttttgtaca agaagaaaac caattgaagt cattcagcaa agtaagtgag
     7861 gacttaacaa aacacaacat catagtcggt acattatttt ctgtgatgat cccgtccttc
     7921 atgctggctg ccaatttggc tgtggttggg gcgatctttt ttgtgagcga tcttgttaaa
     7981 gatgatccta cgttaattgg tggaattgct tcattcatga actacttgat gcagattatg
     8041 atggcaatca ttattggcgg aatgatgatg atgatgactt caagagcagc cgtatcgctg
     8101 aagcggattg cggaaatttt agatgctgaa ccagatctta cttatttaga cgttccagaa
     8161 caagagttga cgggttctgt cacttttgac catgtttctt ttcgttatcc tggtgaggat
     8221 acagatacgc ttaaagatat cagcttttct atcaaacctg gagaaatggt tggtatcgtc
     8281 ggggcgactg gtgccgggaa gtcaacacta gcacaattga tccctcgtct atttgatccg
     8341 acagaaggca aagttgaagt aggtggtgta gatcttcgtc aagtaaatga aaagagttta
     8401 agacaaacgg tttcctttgt tttgcaaaaa gcaattttat tctcaggaac gattgctcaa
     8461 aacttgcgcc aagggaaaaa gaatgcaaca gaaaaagaga tggaacatgc ttcttctatt
     8521 gctcaagcaa aagaattcat cgaaaagtta tctgatcgtt acgaagcacc aatagaagaa
     8581 cgtagcagta acttttctgg tggtcaaaag caacgtttat ccattactcg tggcgttatc
     8641 ggtaatccaa agatcttgat cctagatgac agtacgagtg cactggatgc tcgctctgaa
     8701 aagctggtcc gagaagcact cgatcgagat ttgaagggaa caacgacgat tgtcatcgca
     8761 caaaaaattg cttctgttgt caaagcagac cgcatcttag tattggatga aggcagatta
     8821 gtcggcgaag gaacccatga ggaattggta gctggaaacc gtgtctatca ggaaatcttt
     8881 gaaactcaaa aaggaacgga ggagtaagta tgactgatct aataaaagca agtaaatttt
     8941 tctatcacta tttgaagcgg tacaaactct cctttttctt tatctttatc acagtgatca
     9001 ttgctaccta tctacaagta aaggcacctc aatacatcgg ggaagctttt caagaattag
     9061 ctaattacgt aggaggcttg atgcaaggcg tagacgataa aagcaaattt gtttctgtaa
     9121 tatggaaact cttgctgttt tatgtactag ctagtgcagc caactttatc tatagtattt
     9181 tgttcacaca agtagtaggg aagtcaacga accgaatgcg tatcggtcta ttcaacaaat
     9241 tggaaaaatt gacgatccgt ttctttgatt ctcatcaaga cggtgaaatt ttaagtcgtt
     9301 ttaccagtga cttagataat attcaaaata gtttgaacca agctttgctt caagtcatca
     9361 cgaatgctgt tttacttgtc gggatcttga tcatgatgtt ccgccaaaat gtagagttag
     9421 cttgggctac aattgcttcc actccagtag ccgttttgat agctgtcttg attatccgca
     9481 aagcacggaa atatgtggat cttcagcaag atgaagtggg gaaactaaat ggttatatgg
     9541 atgaaaaaat cagcggacaa cgtgtcatca tcacaaatgg cttacaagaa gaaacgattg
     9601 aaggattctt agagcataat caaagtgttc gcgaagcaac cttcaaagga caagcttatt
     9661 ctggtctact atttccgatg atgcaaggaa tgtcactggt caatacagcg atagttatct
     9721 ttttcggcgg ctggttagca ttaaatggtg atttggaacg ttcggtggca ttaggcttag
     9781 ttgtgacatt cgtccagtat tcacaacaat attaccaacc actgatgcag atttcatctg
     9841 gatacagcat gatccaatta gcgattacag gggcaagacg attgaatgaa atatttgatg
     9901 aaaaagatga agtcaaacca gaaaacggta aagcatttga tggtgttgaa aaaggtcttg
     9961 cgttgcagca cgtagatttt ggttattctc cagaaaaact agtcttgaaa gatgtctcga
    10021 tcaatgtgga caaaggagaa atggttgctc ttgttggtcc aacaggatcg gggaaaacga
    10081 cgatcatgaa tctgctaaac cgtttttacg atgtcaatga tggttcagta gtcatcgatg
    10141 gaatagatat ccgagattat gatcttgatg cattacgtag acatgtagga atcgttttgc
    10201 aagaatctgt attgttctcc ggaacgatca aagacaatat cacattcggc aaaccggacg
    10261 cttcagatga agaagtcatc aatgcagcaa aacaagcgaa tattcatgac ttcatcatga
    10321 cgttagaaaa aggttatgat acagagatat ccgaagaaaa taacttattc agtacaggtc
    10381 aaaagcagtt gatcagtatt gctcgaacga tcatcacaaa tccatcttta ttgattttgg
    10441 atgaagcaac aagtaatgta gacacggtaa cagaagcaag gatccaaaaa gcaatggatg
    10501 aagcaatcaa aggaagaacc agctttgtga ttgctcaccg gctgaaaaca atcttgaatg
    10561 cagatagaat cgttgtcttg cgtgatgggg aagtcattga agaaggcagt catcatgagt
    10621 tattagcaga aaatggattt tatgcggaac tttatcataa tcaatttgta tttgaaagtt
    10681 aaattaaaag aacctcgtta taacggggtt ctttttatat attttatgtt gtttgttttt
    10741 ataaggggca gaaaaggggc aatttaaatt tctatttttc ctagtttttc tactagttga
    10801 ttttgcatat ttttagttgt atgagtataa atctttaatg ttgtttttga atcttcatgt
    10861 cctacacgat ccataatggc ttttaatggg attcctaatt ctgtaagtag agaaatgtgg
    10921 gaatgcctta acatgtgaga agaaactttt ttatctaggt ttaattcttt ggatactgct
    10981 cttaaagatt
//
LOCUS       DACDOA010000085        10974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDOA010000085 DACDOA010000000
VERSION     DACDOA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.085"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(39..230)
                     /locus_tag="IWI39_002126"
     CDS             complement(39..230)
                     /locus_tag="IWI39_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209192.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(371..691)
                     /locus_tag="IWI39_002127"
     CDS             complement(371..691)
                     /locus_tag="IWI39_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209193.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(714..1148)
                     /locus_tag="IWI39_002128"
     CDS             complement(714..1148)
                     /locus_tag="IWI39_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9209194.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1483..2121
                     /locus_tag="IWI39_002129"
     CDS             1483..2121
                     /locus_tag="IWI39_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9209195.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2445..2528)
                     /locus_tag="IWI39_002130"
     tRNA            complement(2445..2528)
                     /locus_tag="IWI39_002130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Leu,seq:cag)
     gene            3056..3556
                     /locus_tag="IWI39_002131"
     CDS             3056..3556
                     /locus_tag="IWI39_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209196.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(3746..4090)
                     /locus_tag="IWI39_002132"
     CDS             complement(3746..4090)
                     /locus_tag="IWI39_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9209197.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(4244..5164)
                     /locus_tag="IWI39_002133"
     CDS             complement(4244..5164)
                     /locus_tag="IWI39_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9209198.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5351..5842
                     /locus_tag="IWI39_002134"
     CDS             5351..5842
                     /locus_tag="IWI39_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9209199.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(5866..8241)
                     /locus_tag="IWI39_002135"
     CDS             complement(5866..8241)
                     /locus_tag="IWI39_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9209200.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            8567..9193
                     /locus_tag="IWI39_002136"
     CDS             8567..9193
                     /locus_tag="IWI39_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9209201.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            9216..9920
                     /locus_tag="IWI39_002137"
     CDS             9216..9920
                     /locus_tag="IWI39_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9209202.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            10112..10945
                     /locus_tag="IWI39_002138"
     CDS             10112..10945
                     /locus_tag="IWI39_002138"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9209203.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         3263 a         2150 c         1906 g         3655 t
ORIGIN      
        1 agatgcagca aatttctgcc gatctttaat cgttttttct attctgttat accaaaaaaa
       61 tgtttgaatc ggtgaaggat cgttccttgt tttaattcta taaattcttg tgcgatcggg
      121 tacttttctt ccaaattttc tttgttcttt tcgatcaggc ttttcgcttc ttcatatttt
      181 ttatctgcac atagtgcttt tacttcctgt aatacttttc ctgcttccat ttgcctcacc
      241 ctcttgccat tttattgctt taagttttat ttaagttata cgcattattc tagtttcatc
      301 ccaaataacc ttttttcatt tttactcctc gaactaatca gttttctaaa ggctgattgg
      361 ttcgtctttt ttatttttcc tccatttcat tctgctcttc tgttaattta tctaatgctt
      421 tgattgatgc tgcggctctt gcataatcat agctgtataa ttccataaat ttatcaggca
      481 gaacaaacca cgctggatga taaccgttag ctgtgacagg gaatccttta tcttgttggt
      541 tatctccatc ccatctcaga gcaacacctt tttttccttt ccactttact aaagcgatcg
      601 agtatccctt ctttgtttgt gtgccgtctt caataattgc taaaatttct attttttctt
      661 ttggcgcacg gacttcttga ggacgaacca ttttctattc ctcccttatc tcattacttc
      721 acgaccaaca cactgcaagg ggcatgtgat acgacataag aagcggtcga tccaactaat
      781 gcttgttgta tagcaccttt tcctgtcgct cccataacga tcaaatcaat attttcttgt
      841 ccagggatga cattggcaat caaatttctt ggatctccag tttcaactat tgcgtggatc
      901 tcagcaatgc cgtactcttt ggcatcattg atttttttca gcatggctac ttctgttctt
      961 tgtttttcat ctgcatagat ttttgaaaag gcataggcac tatgggtcag ctcaactttg
     1021 ttgatgattg acacgataaa taagtgggct tcattgtcct ttgctaagtc aagggcttca
     1081 gcgaacgctt tgatcgattg tcttgatccg tcaactgcta ccatgatgtt ctggtatctt
     1141 ttactcattg gaaccgcctc ctttttcttt tattgtaaca tctcaagcaa aaatacaaaa
     1201 aatatacgca atatgtaaat aaaatgatga acaaaagcaa tttctttcaa gttatttata
     1261 catttctttt ctcatcagaa cacctgttct aatcccatta tttctgcatt atgtgaaatt
     1321 cctcatcatt tttcacatcg agcgaacatc attctgttac aaaaaacgtg gatttgtcat
     1381 aaattgtcat gcttctttta cctatgaaat attttcacgt gttaaagtat tcctcgttga
     1441 acacaacaaa taaaactttt taacgaataa aggagatttt tcatgacatc acttaaaact
     1501 ttactttttg gaacaacttt ggctgccggc gctgcattct tcatgggaac gactgctcat
     1561 gcggacgaag cttataccgt tcaatcaggt gatactctat caacgatttc tcaaaaatac
     1621 gttggtgata actccttgat caatgccatt gcagaatcaa attcaatttc tgatattaac
     1681 ctgatctact caggacaaca attaacaatc ccaacagaag gctctgcaca agcagctgct
     1741 gaacctcaag cagccgttca agaagcacca gtccaagctg aacctgttca agctgaacaa
     1801 cctgttgtac aagaaacggt tcaaacggaa acacaagcag cgcctgttgc tgaaacacaa
     1861 cctgctcctg ctgtaacaga aactgctgct actccagcaa gtacaagctc tgcaaaagaa
     1921 tggatcgcac aaaaagaatc aagcggttct tacacagcaa cgaacggacg ttacatcggc
     1981 cgttaccaat tagattcttc ttacttaaat ggtgactact ctgctgcaaa ccaagaaaga
     2041 gtagcagaac aatatgttac ttcacgttat ggttcttggg aagcggcgaa agcattctgg
     2101 gaagcaaacg gttggtacta agattcatta tttgaacgtt taaacacatt caaaatatag
     2161 taagaggtca tgcgatttat tctttcgcat gacctctttt tgagtttaag tttagtcttt
     2221 attccttaac tatcgtttgc cagtatttct tgtatcagaa tctataaatc tactatttga
     2281 atcaatctat taggttttaa actgtcccac cttcacacaa attttttttg atttatttga
     2341 atctggttcc gttgtttaaa atttttccta tattctttta gataatatgt tattttcttt
     2401 ctcatcgctc caggtgtacc ttctctttat aaaaaaacaa attatgcggc taagaagact
     2461 cgaacttcca cgaggtcacc ctcactagcg cctgaagcta gcgcgtctgc caattccgcc
     2521 atagccgcat cacatctttt agtttaggat tacatgtgtt ttttgtcaag aacatatttt
     2581 tgttcttttt catttctgta cgccctccct tatgccatct acacaaataa ttcatccgag
     2641 caactccatc atctattgag ggttggtgaa aaaatctgtt ttatccatat gatcgatttc
     2701 aatgttaaac gaacgactct gggattagta actattttat ttttttcttc atttcttttt
     2761 tcctcttttt ctcgattcag ctatcttctt tcatttggat attgattatt tcttctgttg
     2821 attactatcc ttctggaatt ctagacgctg aagtaattca cgttttcttt cttctagaga
     2881 tcataagtaa cgaatgaacg aacttcaaat aattttttta ttgtaattca tatttgtatt
     2941 gttcgatgat tcttaatacg atatgacaca ataaataaaa ataggcatgg ctatttacaa
     3001 caatagtgct atagtagaaa acaaatgaat aaggttactt aaagaggtga atacgatgaa
     3061 actgatcaaa ctatgggaag cagatttaaa gaaagcttat cagctacaaa ctagctttgg
     3121 accaaatgag agtggattta tcaacaatgc ttatggctat acatttgctg aatttttaga
     3181 ttatgtaaaa aaatgcgaaa aaacgtcttt aggaatcgat cttcctgaga gttacgttcc
     3241 cgcaaccgtc tttattttag aaagtgatga acatgagtat gtaggaattt ttaatttgcg
     3301 ccataaactg aacgaaacat taagaaacgg cgctggccat attggttacg gcatctcccc
     3361 taattatcgt aaaaaaggat atgccacaca aggattagca ttagtactac aagaggcaaa
     3421 aaaaatcggc attgatgagg cacttttgtc tgtaaataaa aaaaatcttg gcagtcttaa
     3481 agcacaacaa aaaaatgggg ctgcgattta tcgtgaagat gagaaagaat actatacgaa
     3541 aatcaaactg atgtaaaaaa gaaggattcc aaaaggaaag ggatttgaat gtcctttatc
     3601 aatagaacac atttatttaa ttaacttata atatagcaga caaaatttta taaacaaaga
     3661 aaccttttat ctcttttctt ctagaaagga taaaaggctt ttttgttttt cctatttgct
     3721 tctcatagct agtcagccaa gatttctatc gtatccccaa cgcaatccgt gcataacggc
     3781 tcatcttatc tgtcgtccat gctggatacc agactagttt gacttctgct ttcgttactt
     3841 ctggcacttc ttttaatgcg tcatggatag attctgttaa aatatctgct aatggacagc
     3901 ccatagttgt tagagtcatc ttaatgactg tttctccatt ttcaggattg aattctatat
     3961 cataaatcaa gcctaaattg acgatgtcga ttcctaattc aggatcaatc accatttcca
     4021 aggcagcaag aatacgttct ttgattagtt ctatttcttg tccagaccgt tcttggtttg
     4081 cttcgctcat cgtacgacac tccttaacac gatttttatt tatcatactc tttttttagc
     4141 tgcttgtaaa tgagaattta gaagtaagat tctcgtttaa atttataaaa atgttcttat
     4201 tagatgtaat agattaaatt tattctaaaa taaacctgat tcctcatttt ttttgtgaac
     4261 gattttttat ttctggcaat acgccagttg ttcgatacat tttttgttcg ttttcatttg
     4321 caaaccggcc aaatactgaa tgagttggta aaatattgat aaatgcatcg tcgtcgattt
     4381 catgaacgat ctgatcaaga tcatacattt cgtagcgggt gatgaccatc atgatcatgt
     4441 tcccttgttt ttttgaatag ccaccgacag acggcagtaa tgtcattcca cgtaccatcc
     4501 gttttgctag ttcttctgtt acctcttctg gcttgacggt aacgatcatt gctgttactt
     4561 tttgatgact cgtgtggatg gcatcaatta cttgagtcat tgcataaatg gaaataatcg
     4621 tatacaaggc actttcccag ttaaagaaga agcctgcgat gactacaatg ataccattta
     4681 gaatcagcat gtagtttccg actgtttttc cagttgtttg tgaaagtaca agagagatga
     4741 tatccatccc accagttgta aagcccattt ttaaagataa tcctacgcca agacctaata
     4801 aaacaccgcc tactagggca ttcatcaaaa tattatcagt gatctgtccg actggaagca
     4861 gcatcgtcat caccgataca catacaacat tcacaaaact aaggatcgtt gctcgttttc
     4921 ctactttgag aaaccctaat acaaacacag ggatattcaa aagaaaaata aatgttcctg
     4981 tgtctatctt gatatgaaac cattcatata aaagcgagga aatgatttga gcgatcccat
     5041 tcatccctgc agaaaagaca ttggctggaa tcaaaaaata atttaaagcg accgcagcgg
     5101 tcaatccggt tatcaaaata accactattt tctttaatct ttcattttta tgatacgcag
     5161 tcataatttt actcacaagc acctctcccc tactttggta ttccaaatgt aagaataaca
     5221 gtttatctat taaaataaaa tacttcgaac tcaaatgaga agaattttag caaacaggta
     5281 agaatctgcc ctaatcctat gataagatag atgtatggta aatatttata tgtatatcag
     5341 gagggttcct atggcaaaag atgctagctt cgacattgta tcagaagtca atacagaaga
     5401 agtaaaaaat gcgattcagc aaacaacaaa agaattgtcc aatcgctttg acttcaagga
     5461 ttctacagta gaaatcaaat tagagaacag caatctagtt gtattgagcg atgatgattt
     5521 caaaatcgaa cagatcaaag atgtattatt tggtaagctg atcaaaagaa atgtgccaat
     5581 caaaaatatc catttttccg aatcagaaca cgctctaggt ggcaaagctc gacaaactgc
     5641 cgatttggtc aacggaatcg atcgtgaaaa cgcgaagaaa atcactacag ctatcaaaaa
     5701 tgaaaaactg aaagtaaaag cacaaatcca agaagatcag attagagtga caggtaagaa
     5761 ccgtgatgat ctacaagctg tgattgcttt attacgtaaa cttgacttac caatcgagtt
     5821 gcaattcact aactatcgtt aatcaaaaaa cgaggctgac gtctgtcatt cctcgttttt
     5881 ttgatttact gcttcttttg ataaccaaaa acgctgtatt ccttcttcca attccccaac
     5941 acttcgtact tctcttgctg tttgccaatg ttgtgggaat aaacgtcgat aagaaaaact
     6001 agaaaatcgt tgatctgcca atacaacgat tccttgatcc gccatgccac gaatcactcg
     6061 accaccagct tgcaagactt tgttcattcc aggaagctga taagcgtaag caaatccaag
     6121 gtgttccttc tcatcataat atgatttgat cagttcctgc tcatgattca tctgcggcaa
     6181 acctacacct acgatcatac tgccgatcaa tcggtcccct ctcaaatcga ttccttctga
     6241 aaaaatcccg ccaagtacgc aaaatccaat caatgtttca gcgggatttg tttgaaattt
     6301 tgttaaaaat cgttcgcgtt cttcctcatt catgtccgtt tcttggatta gcacattgct
     6361 gtcgggatat tgctgtctga atgcttccac cgcaagatct aaatattgat aagaaggaaa
     6421 gaaaacgaaa taattcccga cctttccatc agcaaaagca tgaatcgtct ctactaattt
     6481 tgccagactg tgttcccttt ttcgataagt cgtctctagg taatttgcga tgaaaacttg
     6541 ctggtttcct tctgggaaag gactcggcag tttgtaagcc agactttttt gtccccccag
     6601 tgtttcctga taataagaga gtggtgaaaa actagcagaa aaaagaatac tgctttttcc
     6661 tttatccaac gactgctcaa gaaacaagct tggatcaata caaaactctt ttactatcag
     6721 atcgcgatac gttttttcga ctgttgtttc gtagtgatca tcataaaatt cactgacctt
     6781 caaaaaatga agcaaatcaa aataataagg aagcaattgt tcttgtgatg ggtgctcagg
     6841 aaattctgcc agccattctt taattttttc gcttaattga taacctgcct ttactaaaga
     6901 ttcagccggt gctttttgat gatgataatc ccaatgatct tcttttgcta tttctcggat
     6961 cgaatcaaat tcttttaata gtttgttgaa acggcgatga agttttttga attcctttgg
     7021 tatagcttcc ttacttcgtt tcgttttttc ataagatagt tctgccgaat acatctctcg
     7081 ggaacgattc accaaattat gtgcttcgtc aatcaaaaac agatagtcct cgtttttttc
     7141 atcttcaaaa aaacgtctta aataaacagt tggatcaaat aaataattgt aatccccaat
     7201 gattacgtca caccagatac tgacatctaa tgatagttcg aaaggacaaa gtgtatgctt
     7261 tcttgcataa gtttcgatga cttcacgagt gatctgattc tcatgattca aaagatccca
     7321 taaaccttca ttaatccgat tgtaataacc attggcatat gggcactgat ctggattaca
     7381 ggtcgtctca tctaaaaagc agatcttgtc tttcgccgtc aaagtgacac tttttacttc
     7441 agctcctgta tcttttaatg ctgtcatagc atcttcagct acttgtctag ttattgtttt
     7501 agccgttaga taaaagatct tttctccctc ttcttcccct acagctttca aagcggggaa
     7561 taacgtagag attgtttttc ctgttccggt aggtgcttct acaaacaatt tctgtttcgt
     7621 acggatcgtt ttataagcag ctaccgctag ttcacgctga cctttcctat actcatcatg
     7681 ggggaatttc aaagccatca aagaggcatt gcgtacagtg cgccagtttt cttgaaaaac
     7741 aagccattct ttataatctt cagtcagctt tttaaaaaaa gtttctaact gctctcttgt
     7801 ctgatgttca acctttctcg tgatcacttc ttctgccgtc tggaagtaag tcaattgcag
     7861 atttatttct tgtaaatcat gttcttgact atagatatag gcatacaccc ttgcttgatg
     7921 aaagaaaagt tccacttgtt ctggttctaa atcttcaaat cgtggttcgg aggttttgat
     7981 ttcatctata taataaatac cgtctctttt gaaaataccg tctgcacggc cttcaacgat
     8041 caggtaatat tcatctaatt gaactgttgt ttttaaaaag acttcttttt catagttttc
     8101 tcccgcatct ttttggagct tgcggtggat tctcgctcct tctaatgcag tatggttact
     8161 ttttttacga ttgtcgatac ttccacgtcg caaaataaat tctactaagt gccgcacaga
     8221 tatttttgac tgtttgatca tttccaccac ctctttctct ctaagattac ctgaaaaaac
     8281 ctagtttagc aaaaagagct ctctttccaa acataaaaaa acaccctgct gattaggcag
     8341 ggatatagga tatagaattt ggatttttgg atgaatctaa tgtaacaagg acatattaaa
     8401 atgctatgaa aacttacttt ccttctcttt agaatatcca aatacttttc atatagtctg
     8461 ataattcaga taagtaaaga gaaataagta cctgtttttg tttcctagca atgaatgcgt
     8521 tatactaatg gatgaaagaa gttttggcta tcaaggagga atgcacatgc atatcgaaag
     8581 attaaaaaca ggaatgattg aagaaaactg ttatttggtc tacaacgaag aagcactttt
     8641 gatcattgac cctggagaag atgcggaacg aatcaaacaa gaaatcaaaa aaacaaacca
     8701 acagccggta gcgattttgc ttacccatac tcactacgat catatcggtg cagtggaacc
     8761 tttacgcgcg tattatcaaa tcccagttta tgtcagccca ctagagcaaa aatggctagg
     8821 tgatccgatt ttgaatcttt ctggactcgg tagacatgat gatattgctg atgttattgt
     8881 ctcgcctgca gaatatgaat ttgaaatgaa aacttatact ttaggaaaca tgtcttttcg
     8941 cgtcgtccct accccagggc attctatagg aagcgtcagc tttatttttg atgattttgt
     9001 cgtttctggc gacgccctgt ttagaggaag tatcggtaga accgatctgt atacagggaa
     9061 tttagaacaa ttacttcata gtattcggac ccagctgttt gtattacctg atgaatttgc
     9121 agtctatcca ggacatggcg atgcaacgac gattgaacag gaaaaaagga caaatccttt
     9181 tttcaatcgt taaaacgact taggaggaga ataaaatgac agaaactaca ctttatatcg
     9241 tacgacacgg aaaaacgatg ttcaatacaa ttgaacgagt acaaggctgg tgtgatacac
     9301 ccctcacaaa gcaaggacaa gaaggcattc attatttagg tcgcgggtta aaaaatgtcg
     9361 acttttcatt tgcttattct agtgacagtg gtcgtgcaat cgaaactgcc cgtattattc
     9421 taggagaaca ttctaaggga aaagaaattc cttattttat tgaaaaaagg attagagaat
     9481 ggtgtttcgg gtctcttgaa ggtggatatg acatggaaat gtggggtgtg atccctcgtg
     9541 tattgaattt taaaacatat gatgaaatgt ttaccacaga agtttctttt gaacaaattg
     9601 ctaatgctgt atacaactta gataccgctg ggtgggcgga aacttatgaa acattgcgag
     9661 accgtgtatg gacaggattt gaagatatcg cccatcactg cgaaaaaaat ggtggtggaa
     9721 atgttttagt cgtatctcat ggtttgacga tcgccttttt gcttaatctg atcgatccta
     9781 aacagcctgt tcgtgctggg cttttaaatg gcagcgtaac aaaggtcact tatgcaaacg
     9841 gtcgattttc tatccacggc atcaatgata cgtcttatat cgataacggg aaaacacata
     9901 gtcgcgactc gtttctataa tggggatatt ttaattttta ccagagaatc ataaaaggca
     9961 aagtctgaac gagggctttg tcttcttttt tctcttcgtg tacttatctg ctttatttct
    10021 caagtttttc ttaatattaa aaaacagatt gggtttctca aaaaataacg gtatactata
    10081 agatgtatct taattgggaa ggaaggaatt tatgtttttt gcaaatatta tcaaagcagt
    10141 gattctcggt attattgaag gaataacgga atggctgccc atcagtagta ctggtcattt
    10201 gatcctagca gatgaattca tcaaactgga tgccagttca caatttatgt cgatgttcaa
    10261 tgtcgttatc caattagggg cgatcttggc tgtcgtcgtt ctttattttc acaagttaaa
    10321 cccattttct ccaagtaaat caacggtaga aaaaaaagat acgtggacac tttggtttaa
    10381 agtgatcgtt gcttgcatac cagccatcat cattggtttg cctttggatg attggttaga
    10441 agcaaatttc cacaagttct taccagttgc cattgttttg atcgtttacg gtattgcctt
    10501 tattattgtc gaaaaacgca acaaaaacaa aacacctcgt tggacttctc tgaatgactt
    10561 tacgtaccaa gcagctcttc ttgttgggtt attccaagtc ttggcacttg ttcccggaac
    10621 atcccgttct ggcgcaacga tcctaggatc tatcttgatt ggtgcttcgc gttttgtcgc
    10681 tacagagttt tcatttttct tagggattcc tgtcatgttt ggcgcaagcg gcttgaagat
    10741 cgtcaaatat ttagctgaag ggaattcatt caaaatggaa gaaactgttg ttcttcttgt
    10801 aggaacattc gtatcttttg tggtttccgt cttagctatc aaattcttac taagctattt
    10861 gaagaaaaac gactttaccg cttttggctg gtatcgtatt atccttggta tcattttgat
    10921 tggttattgg tttattgcaa tgtagaaaaa accagaacac ttctcttatt tgag
//
LOCUS       DACDOA010000086        10915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDOA010000086 DACDOA010000000
VERSION     DACDOA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.086"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..499
                     /locus_tag="IWI39_002139"
                     /pseudo
     CDS             1..499
                     /locus_tag="IWI39_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(401..1117)
                     /locus_tag="IWI39_002140"
     CDS             complement(401..1117)
                     /locus_tag="IWI39_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9209204.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1167..1376)
                     /locus_tag="IWI39_002141"
     CDS             complement(1167..1376)
                     /locus_tag="IWI39_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209205.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1653..2926
                     /locus_tag="IWI39_002142"
                     /pseudo
     CDS             1653..2926
                     /locus_tag="IWI39_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3033..4064)
                     /locus_tag="IWI39_002143"
     CDS             complement(3033..4064)
                     /locus_tag="IWI39_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209206.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4071..4901)
                     /locus_tag="IWI39_002144"
     CDS             complement(4071..4901)
                     /locus_tag="IWI39_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209207.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4898..5704)
                     /locus_tag="IWI39_002145"
     CDS             complement(4898..5704)
                     /locus_tag="IWI39_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9209208.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5710..6534)
                     /locus_tag="IWI39_002146"
     CDS             complement(5710..6534)
                     /locus_tag="IWI39_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9209209.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6524..7624)
                     /locus_tag="IWI39_002147"
     CDS             complement(6524..7624)
                     /locus_tag="IWI39_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9209210.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7802..8587
                     /locus_tag="IWI39_002148"
     CDS             7802..8587
                     /locus_tag="IWI39_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9209211.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8620..9003
                     /locus_tag="IWI39_002149"
     CDS             8620..9003
                     /locus_tag="IWI39_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9209212.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9079..9210
                     /locus_tag="IWI39_002150"
     CDS             9079..9210
                     /locus_tag="IWI39_002150"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9209213.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9179..9415
                     /locus_tag="IWI39_002151"
     CDS             9179..9415
                     /locus_tag="IWI39_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9209214.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9493..10464)
                     /locus_tag="IWI39_002152"
     CDS             complement(9493..10464)
                     /locus_tag="IWI39_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9209215.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3247 a         2529 c         2096 g         3043 t
ORIGIN      
        1 tactcggatt aacagataaa catttatttt ttgatgaaga atggctgatt gaaaaagaat
       61 ataaaggcgt tcaagcgcag tttattaaag ggagattgga tgactcacct tcccattgtg
      121 aacactgtgg atcgattcgt attatccgca acggttccta tacgacacgt actcagatgt
      181 tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt ctatgctatg
      241 attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat caactaacaa
      301 aggaactgaa acaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt aatctgaaaa
      361 tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag attccacgac
      421 attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta gtcaaacaga
      481 aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca ccttccatat
      541 ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca accacgccag
      601 tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct tgtaatttag
      661 gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata ccaatctcat
      721 tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc catggcagct
      781 ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg aattcgactg
      841 gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct gtcggcccgc
      901 acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca tcaactgtca
      961 tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc tgttcaaccc
     1021 tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc tgaagatcat
     1081 cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca aaaaaatagt
     1141 ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct gatccgctgc
     1201 tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc ataaaaggac
     1261 agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt atagccgaac
     1321 tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac atccattcac
     1381 tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc tttccccctt
     1441 acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata aaaactgccg
     1501 tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg atagtaaatc
     1561 attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg ttagaattga
     1621 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt atcaaactat
     1681 tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa aaaatacatt
     1741 caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc tgtcctcagt
     1801 gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa tcgctggtcc
     1861 ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa cggtacctct
     1921 gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa aactgtttta
     1981 tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa gcaatgacga
     2041 cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc gcttctctgc
     2101 tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta gatgaatttc
     2161 gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc gatactggga
     2221 aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat tttatgcaat
     2281 ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac gcctcttatg
     2341 ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc tttcacatcg
     2401 tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa agcttcgacc
     2461 gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct cgacttctga
     2521 tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat tttggttggg
     2581 cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat gaactacgaa
     2641 tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa gcggacatct
     2701 tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat catataaaaa
     2761 aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct tactcaaatg
     2821 gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct tacgggttag
     2881 gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat aaaaaaacat
     2941 ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg
     3001 acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta
     3061 tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt
     3121 ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa
     3181 acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc
     3241 ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg
     3301 cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag
     3361 ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc cttcgaggtg
     3421 gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc
     3481 tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc
     3541 aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc
     3601 agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg
     3661 ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc
     3721 tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct
     3781 acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata
     3841 ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc
     3901 tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga
     3961 cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt
     4021 ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc
     4081 tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc
     4141 ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac
     4201 aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg
     4261 cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc
     4321 gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc
     4381 tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc
     4441 agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa cgccgtaata
     4501 aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata cgttcgaagc
     4561 aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag cttttccagc
     4621 caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat catcaacaac
     4681 agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc agcaataaat
     4741 tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg taaaagcgat
     4801 tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag caagtaatac
     4861 aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc accttgcctt
     4921 tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact gccaacgcac
     4981 tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt cgaatcgtta
     5041 aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc atgccattag
     5101 caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga
     5161 accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat tcctttttca
     5221 cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa taggtatacg
     5281 tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc aaaatcattt
     5341 tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga atcccagcaa
     5401 aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc gatagattca
     5461 ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag ctgacagcaa
     5521 tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata tcactgtaat
     5581 tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc ataaaaagca
     5641 gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc aagcgttttt
     5701 tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg acggtgcaat
     5761 tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt gttccttcag
     5821 cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc
     5881 gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt gataaccttt
     5941 ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa ccgctgctgc
     6001 atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca tcgaatgtac
     6061 gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa ataaatgcga
     6121 atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct cctcctgatg
     6181 ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc tgtaccagtg
     6241 gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca gcgaccactc
     6301 tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg gaacgccggt
     6361 catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta ccttcaataa
     6421 gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga cggctgtgtc
     6481 gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt gcataggtca
     6541 gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa gcttcattgt
     6601 cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca gctacttctt
     6661 taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg atttgccctt
     6721 catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg gcatttttat
     6781 tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt acctcaaaac
     6841 gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca actgccacat
     6901 cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa cggccttttt
     6961 ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca cctccgttgg
     7021 caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca tcccccacgc
     7081 taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc ttcacattct
     7141 ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc ccatcatcat
     7201 cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag gttaactctt
     7261 tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg gttccatttt
     7321 ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta tccacgtgac
     7381 ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc caagtatccg
     7441 gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc gtatctaaag
     7501 agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct gtcagcagga
     7561 tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt tcattcattt
     7621 tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta atgagagaat
     7681 cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg taaatttcga
     7741 cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa ggaggctccc
     7801 tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag aggaaaaatt
     7861 tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact ttgatttaaa
     7921 caatccatct accggaaaag atgacattcc ggattatcca gcctatataa acaaactgga
     7981 ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc
     8041 aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt taaagctggt
     8101 aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa ccaaagatcc
     8161 cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg attttccaga
     8221 aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca cagcggaaga
     8281 attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg ttgaacgaga
     8341 attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag acctttaccg
     8401 ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag gctcggatgc
     8461 tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt tggcagactg
     8521 tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc cgctgccgat
     8581 tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct gtgggactac
     8641 aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt gaaaattgag
     8701 gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa ttttttggtg
     8761 gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa agatcagcct
     8821 ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc agatgactac
     8881 aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc ctataccgtt
     8941 ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat tttcgatatt
     9001 tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt agaaaaaccg
     9061 cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct ccgcttattt
     9121 cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca aggcgtttat
     9181 gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg gcgctttgga
     9241 tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg aagtgcatcg
     9301 cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc acaccaacgg
     9361 caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa tgtaaagcaa
     9421 gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt ttttggacag
     9481 gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa cagtgaagat
     9541 gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc gtaaaaagac
     9601 actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg agtgtctttc
     9661 atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg ttaaagctgt
     9721 gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca tttctttagg
     9781 tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt aaccgtgcat
     9841 accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat ctgccacaaa
     9901 acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca caggaactcg
     9961 aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct tcaaaatttg
    10021 ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt ttacatccac
    10081 aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac cacaaaagct
    10141 ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt ctttagcaaa
    10201 atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg agtgaaagta
    10261 aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg tctggccaaa
    10321 tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac agcgtaatgg
    10381 gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag aaccctgttc
    10441 tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt taaactaaaa
    10501 cacacaagga aaaacttacc atttaatcat aaaattagct catataaata tactaggatg
    10561 taaaatagac tggtcaaaat ctggtcaaaa aagaggacta atacaggtag tcctcttttt
    10621 tgttatttta atattctaaa tcatttttta ttggtttatt cccttattcg atgtagactc
    10681 aaatcactat atcactagat atcaaacggc gtcatacgga atcaaaaaca tcaaatttga
    10741 cctgttaaat ggtcattgaa taacattcaa aatcaataaa acggagtcag acacggagtc
    10801 aaaaacgctg agagtaaaaa atatatacag ccttgtaggt aaagtaccac agggctgtat
    10861 attttttatt tcagattacc ttaaatgatt ccatttactt tttatctcat ttttt
//
LOCUS       DACDOA010000087        10823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDOA010000087 DACDOA010000000
VERSION     DACDOA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.087"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWI39_002153"
     CDS             <1..782
                     /locus_tag="IWI39_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9209216.1"
                     /translation="YDPEISEAITKALTEQGINLVTGATYERVEQDGDIKKVHVEING
                     KKRIIEAEQLLIATGRKPNTESLNLHAAGVEVGSRGEIVIDDYLKTTNSRIYSAGDVT
                     LGPQFVYVAAYEGGLAARNAIGGLNQKVNLEVVPGVTFTSPSIATVGLTEQQAKEKGY
                     EVKTSVLPLDAVPRALVNRETTGVFKLVADAKTLKVLGAHVVAENAGDVIYAATLAVK
                     FGLTVGDLRETMAPYLTMAEGLKLAVLTFDKDVSKLSCCAG"
     gene            942..2264
                     /locus_tag="IWI39_002154"
     CDS             942..2264
                     /locus_tag="IWI39_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9209217.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKS
                     IQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRP
                     RLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPH
                     AKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFL
                     LDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVR
                     AVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKR
                     KPRFNSSAFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
     gene            complement(2391..3896)
                     /locus_tag="IWI39_002155"
     CDS             complement(2391..3896)
                     /locus_tag="IWI39_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepS protein"
                     /protein_id="HAP9209218.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRS
                     KKLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQIN
                     TFVIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNY
                     QSIEVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQ
                     WSMKYEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSL
                     ANYASGVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSN
                     WGLESLSEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRL
                     KKTDLQAAITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTV
                     IQVKQQVKSAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
     gene            4600..5490
                     /locus_tag="IWI39_002156"
     CDS             4600..5490
                     /locus_tag="IWI39_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9209219.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQA
                     NATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTEN
                     FDNTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSF
                     EGSQRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVF
                     NTIIQNHARLEWYPRIGFQFQDYHDKKMIALFADIFCELEERITLYKSNGDIQDYFYT
                     PRYILDGKLTSLGKEISLNV"
     gene            5483..5767
                     /locus_tag="IWI39_002157"
     CDS             5483..5767
                     /locus_tag="IWI39_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209220.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIK
                     VSPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
     gene            complement(5800..6039)
                     /locus_tag="IWI39_002158"
     CDS             complement(5800..6039)
                     /locus_tag="IWI39_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209221.1"
                     /translation="MEQSDNVPLDRHKCKEHVFKDKRFVEKESESLTLLGYKIVVDTV
                     TGVNYLETPKGGITPLLDKNGKICIDKKKSINKKG"
     gene            complement(6435..6632)
                     /locus_tag="IWI39_002159"
     CDS             complement(6435..6632)
                     /locus_tag="IWI39_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9209222.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYKQKQGEYVAITIIKE"
     gene            complement(6666..6965)
                     /locus_tag="IWI39_002160"
     CDS             complement(6666..6965)
                     /locus_tag="IWI39_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209223.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(7006..7623)
                     /locus_tag="IWI39_002161"
     CDS             complement(7006..7623)
                     /locus_tag="IWI39_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209224.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(7948..8343)
                     /locus_tag="IWI39_002162"
     CDS             complement(7948..8343)
                     /locus_tag="IWI39_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209225.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(8423..10774)
                     /locus_tag="IWI39_002163"
     CDS             complement(8423..10774)
                     /locus_tag="IWI39_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9209226.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         3494 a         2015 c         1649 g         3665 t
ORIGIN      
        1 aatacgatcc tgaaatttca gaagccatta ctaaggcctt aacagaacag ggaattaatt
       61 tagtaacagg tgcaacctat gaacgagttg agcaagatgg agacattaaa aaagttcatg
      121 ttgagataaa tggtaaaaag cgaattattg aagcagaaca attgctaatt gccactggaa
      181 gaaaaccaaa tacagaatca ttaaacttac atgcagcagg cgttgaagtt ggttcccgtg
      241 gtgaaattgt cattgatgat tatcttaaaa cgaccaattc ccgaatttat tcagctggag
      301 atgtcactct cggtccccaa tttgtttatg tagctgctta tgaaggtgga cttgctgctc
      361 gtaatgcaat cggaggacta aatcaaaagg tcaatttaga agtggttcca ggcgttacgt
      421 ttacttctcc atcgattgca acggttggtt taacggagca acaggcaaaa gaaaaaggat
      481 atgaagtgaa aacatcggta ttgccgttgg atgctgttcc aagagcgctc gttaatcggg
      541 aaacaacagg tgttttcaaa ttagtggcag acgcgaaaac attgaaagtg ttaggggcgc
      601 atgtagtggc agaaaacgca ggagacgtaa tttatgcagc aacattagct gtgaaattcg
      661 gtttaactgt tggagatctg agagaaacga tggctccata tctaacaatg gcagaaggat
      721 tgaagctggc tgtcctaact tttgataaag atgtttcgaa attatcttgc tgtgcaggct
      781 aagggaactt ttttacaact ccctattcaa gtgaaccagc taatgcttta agggattttc
      841 taagtgcatt tgtggtcaca aaaataattt aacactgatg atttcgacat caaatctata
      901 attgatacct taacaccgca gagtaataaa ggatgaataa tatgtcagac ttattatccc
      961 taccagacat taaaacaata gagccgccac aagaaaatga aaccgatatg atgtttaaag
     1021 ttgaagcagt cggaccacct gaacgttgtc ctgaatgtgg ttttgacaag ttgtacaaac
     1081 acagttcaag aaatcaacta attatggatt tgcccattcg tttaaagcga gtgggcttac
     1141 aattgaaccg tagacgatac aagtgtcgtg aatgcggatc taccttctgg gaacgcctaa
     1201 tatctgtaga tgagaagcgt agtatgacca aaaggctttt aaagtccatt caagagcaat
     1261 ccatgtctaa gacctttgta gaagtcgcag aaagcgttgg tgttgacgag aaaaccatta
     1321 ggaacgtttt taaggactat gtggcactca aagaacgtga ataccagttt gaaactccta
     1381 agtggcttgg gatagacgag atacatatta tccgtagacc tcggcttgta ttgactaata
     1441 ttgaacgcag gactatttat gacatcaagc ctaaccgtaa caaggaaaca gtcatccaac
     1501 gtctttcaga aatcagtgac aggacttaca ttgagtacgt cacaatggat atgtggaagc
     1561 cctacaaaga cgcagtgaac cctatccttc cacacgctaa agttgtcgta gataagtttc
     1621 atgtagttag aatggctaat caagccttag ataacgtcag gaagtctttg aaagcccata
     1681 tgagccaaaa agaaagacgt acccttatgc gtgaaaggtt tatccttcta aagcgtaaac
     1741 acgatttaaa tgaacgtgaa tcattcctct tagatacttg gttaggtaat cttcctgctt
     1801 taaaagaagc ctatgaactc aaagaagagt tttactggat atgggatact cctgatccag
     1861 atgaaggtca tcttcgttat agtcaatgga gacaccgttg tatgtccagc aactctaaag
     1921 acgcatataa agacctcgtg agagccgtag acaactggca tgtcgaaata ttcaactact
     1981 ttgataaaag gctcactaat gcttatacgg agtcaattaa cagcattatt aggcaggtag
     2041 agcgaatggg tagaggttac tcgtttgatg ccttacgagc caaaatcctt ttcaatgaga
     2101 agctccataa aaagcgtaag ccacgattta attcaagtgc tttcaataaa gctatgttat
     2161 acgatacttt caattggtat gaagtgaatg atcacgacat tacagacaac tttggtgtcg
     2221 atttttccac acctattaag aatttggaga agggtgattt ataagccctt ttccaccata
     2281 aaatccgaat acccaaaaaa acaaaaatat acagaattag gataataact aacaaaaacg
     2341 aaaacaagta taaaagaaat ataatgtata ggcacttgac atgttttatt ttatcctgta
     2401 ttccacaaat ttaattgctt ataatgatct actctttgac tgtatttatc cggttctctg
     2461 aagattctta ctaacatatc ggcttcttta aaaaatgtct taatgccctg tttgtacgca
     2521 gattttacct gttgcttcac ttgaatgacc gtacgaatta acgctttacg agtggcaact
     2581 actagtcccc cattccgtcc agcttttatt tgtacaaata atttaccttc ggccatcaat
     2641 tcttttaatg cacggtcaag agaacgttta ggaattgatt gtccgttgaa agtaatcgct
     2701 gcttgcaaat ctgttttctt cagtctgact tcaggtctgt atctataaca ttgggtttca
     2761 aggtaagcta aaatatcttc tttccattcg gaaaggtgag atttctttct tttatgacgt
     2821 tccttcttaa atttgtacca agtatttctt tgttgtgtaa acattttttc ctcagacagc
     2881 gattctaagc cccaatttga cactaactcc tgtacataag tcttacttgc acctctatac
     2941 ttcccagagt aagcactttt tacaattctt actcgttctc tatcttttag aggacgttct
     3001 aaagaactgt taaaaagatc catctcattt agacagtctt ctaagtcaac tcctgatgca
     3061 taattggcca aagataaagt gaaaatcaca ttattccggc ccataatccc tttagcgcct
     3121 acaatttttg gattactaat taattgtcga taccacattt cgttcacttg tttatattta
     3181 tttttaacaa gtgaaagtag cggtttttta tatgttttcg ctggctcata tttcattgac
     3241 cattggatta attgctgtat atcatgtgtt aaagctggat aataatagac aatgttgtca
     3301 gttctaggga tacgtgctat cccaaaatga ttgcaaccta aatcgactga aggcaattct
     3361 tcagcaaatg cttttcttaa gttctcagat actcgtttgg ccacttcgat tgactgataa
     3421 ttatttttgg acgaaatata ccaggcattt tcaagaataa agtatgcttg gaagccccct
     3481 ggtgtttcca aaataagggt tggcattaat tccagatcga ttgctgcatc gagaatcatt
     3541 tgagaatcta atttttcgcc tgttaagcga tcaaaatcga ttacaaaagt attgatttgt
     3601 tgcaagttgc gttcgttgtg cccttttaca attattcttt tttcatctgc atatgcacca
     3661 tagctgtaaa cgttaggtgt ccaatgagta aatttatctt cattttcagc taagccttct
     3721 tgagaagtaa gaacgattcc acgagattcc gtcattaact tcttggatct aaacccaaaa
     3781 atagcacctt tttttccttc agctttatag ccgattggtt taattttgct gtttaaacac
     3841 ttgtattcac gaataccact ttttaaaata actgaaatca cgtcagaaac tttcatctta
     3901 tcaccaccca ttctatattt aaaaaaagga gaactgcagg cattctacag ctctccttta
     3961 aaccttattt gcagctaaaa tatagctcaa aatcttgatt tttaaggtcc caaatgatat
     4021 aattaaaaag actaagagag ctgcaactct ctgtaatcta aaactataat gggacgaaag
     4081 agaatcatga aatgctgtgt acattttgat tctgtaattt agttgatcgg caaatcaact
     4141 aagttaccgt ctctattttt ttgtctataa ttcaattagt aaatgtatct tttttctatt
     4201 cattcaaatt cttctttaga atacatgtca ctacaacttt tgtaaagtaa aaattttctc
     4261 tcaaataatc attcttaatt tatttttgta tccttttatc tttgtatcct tttatctttt
     4321 tatcttttta tctttttatc ctttaatttt attgttgtat ctttttatct ttgtatcctt
     4381 ttatcttttt atctttgtat ccttacttag aagtctattt aataactttt cttataaaat
     4441 gctatctgat taaataagta tgttttaaga taattagaaa aaagatacat ttatctattt
     4501 acaattaatt ctatttgtat tactatttaa atatagaaaa aagatacaaa gatacattta
     4561 tcttttatta cattttgatt acaaggaagg agtatattga tggctaatac aattatattt
     4621 ggacaacaaa agggcggggt aggaaaaaca actgacactt gcatgataac gttggtagct
     4681 gcgatgctaa tgaaaaagcg tactatttta attgatattg atttgcaagc aaatgcaaca
     4741 aattttatga caaaaacatt tggggtagaa acggtacctc agactttgat gtctgctctt
     4801 gaaaatggcg atctacgtcc tgctatcgta aaccttaagg aaaacttaga tttaattccc
     4861 gctggttatg atatgcgtct atacactgaa ttcttaacag aaaattttga taatacaata
     4921 gatagaactt tttatcttaa aaaccttatt gaagaaatta aagacgatta tgattatatt
     4981 tttattgaca tcccaccttc aaccgattta aaggtagata atgctatggt tgcatgtgat
     5041 tacttggttg tagtacaaga aacacaacaa ttttcttttg agggaagtca acggctaatt
     5101 tttgattata tacaaacctt agttgacgat tttggctcag acgttccaat tcaaattgca
     5161 ggcattttac ctgcattgtt acaacaaaaa cgcccaatgc accaaaaatt agttgaggaa
     5221 acaatagaca catttggaag agacaatgtt tttaacacta ttattcaaaa tcatgcacgt
     5281 ttagagtggt atccacggat tggatttcaa tttcaagact atcatgataa aaaaatgatt
     5341 gctctttttg cagatatttt ctgtgaatta gaagaacgca tcactcttta taaaagtaat
     5401 ggtgatattc aagattattt ttatactccg agatatatat tagacggaaa attaacttcc
     5461 ctgggaaagg agattagtct aaatgtctag tttgataaat cgtaaaatcc cacaaggggc
     5521 aacagcaaat aaagaagcag atacaaaacg aaaagttact attcaaaaaa aatcgagtaa
     5581 aaagatatta gcttcagaaa gaaaaaacat caaagtgtcc ccctctactt ttgacttaat
     5641 taaaaccata tcaacaatga aaagctacaa aaattatgaa tttattgata aagctgtaga
     5701 aagctatata caaaataatt taacagaacg tgaacaacgt atcctcaaaa atttaatctc
     5761 aaaataaata aaaaaggata aaaagataca tgtatctttt tatccttttt tatttattga
     5821 tttcttttta tcaatgcaga ttttaccatt tttatcaagt agaggcgtaa ttcctccttt
     5881 aggagtttct aaataattca caccagtaac agtatcaact acaattttat agcctaatag
     5941 agttaaagat tcactttctt tttcaacaaa acgtttgtct ttaaatacat gttccttaca
     6001 tttatgtctg tctaatggta cgttatctga ttgttctatc aactcactat ttgaattatc
     6061 cccggcaacc atcatgccta aatttttatc ataataaata tattctccac ctagtattaa
     6121 ggttaatggc ataaaaatct tcctttcttt aattttctta ccgtcctttt tctctttttt
     6181 taacggcttt cgttacttac ctttttgttc attttctacg cacaatcaat cgattaaaca
     6241 gattgcctct tattccaata agtaaatttc aagtgcaaat catcctattt ttaaatagga
     6301 tctgttgtca actttttctt aattctatca aaaaacggaa gattttgctt gcaaaccaat
     6361 tcccttttac taaattaaaa taggttttta tttagtatga aacagaaacc aacattgaag
     6421 taaatcttat ttgtttattc tttaattatt gtaattgcta catattctcc ttgtttctgt
     6481 ttgtatcggc gatggcattt aggacagtaa aagtaatttt tttgataaac actttttgct
     6541 aattccccac agtcacactc cacgtgaaaa gcaatatttc gattaaaact agttatttca
     6601 atgccccaac gatcataaga ttttcgcttc ataaaaattt tcctccttgt aaattttact
     6661 aacgattata aataccgttg ccattctttc aactgttgac taattaacgc ataaaattcg
     6721 acttctgata aaatggtaat ctcatgacct aacgcaatca agtcccgcgc ggtttgttcc
     6781 ttttttgtat agagagaatc atcaaacaac gacttagaat actgaccaca aaccaggaaa
     6841 tttgtttttg aagtaacact tgattgacaa tggccgttta aagagtgaac caaaccgatg
     6901 gcttgggttc gtgtcatttg ctgcagcgtt ccagtaaaaa cgaccctttg attcgttaag
     6961 tccattttca aactctccct tcccgtctaa tagctgttat atctatcaag aaaagtggtg
     7021 caaggtatgt tcatattctt tcacccgtcg ataaaaggta tttcgtttca tgcccaattg
     7081 atccatagca tagactgcag ttatctttcc tgctttccag tcagaataga tcgcaggaaa
     7141 ttcaggaggt aaagcaattt tctttctgcc gaattttaca cccttttgtt tcgcagcttc
     7201 tacgccttct ctttgccggc tattaattcg tttccgctcg tcttcggcca tatagctgag
     7261 taattgcaga attaagtcag agataagcgt ctctaaccca tctagttgct tgtaactagc
     7321 agtatctaac agaggcatgt ctaacacaga aatatcaatc tgcttttcct tagttaattc
     7381 tcgccattct tctaaaatca tttgtttatt tctgcctagt ctatctaatg attgtagata
     7441 gagtaaatct ccttttcgaa acatccgttt cattaattga tattcagggc gtttaaaatc
     7501 tttgccgctg gctttttcta caaatatata ttcttcaggg attcctaatt ccttcgcgga
     7561 aatcagttgt cgttcgatcg attgatcata actactgacc cggatataaa aaaattttct
     7621 catcgttttt atctaccctt ttttcactaa aattgtttca aaacaatcag attaaatcag
     7681 aaacgtttca aattgtatta tgtggttatt atgccataca taaacaaaga aaatcaaggc
     7741 atgtcaaaaa gtatactttt tgaaacagta agtttacact cccgcatgat tatatttatt
     7801 ccgctaaaat acataaaaat ggcaggcctt aacggtctgc cattaaataa actagttatc
     7861 cagtttttct tttatgtctt ccatggtata ttttattttt cacatcagaa tttgacttct
     7921 ttaactttta caattttttt aacactatta ttctattaca acactatata tttctaattt
     7981 agggttaaaa ataaatcttt ttgatacttt tgcctttaca atataattgt attctttctt
     8041 tttttctttg aagtcgcttg attcaactgt aacttcaaac cacatttcat ttggagactc
     8101 ttttaatttt tttacttgaa atgaattatc tcttggatca taaggtggta ctctttcaaa
     8161 ctcaggaagt tttctctgta tttgttcttc aggagtattc gcaaagtaat ctactatctt
     8221 gacagttatt aaaagtacta ctattcccaa aactataaca aacataaaga taattccaca
     8281 tccagaatta tttttattat tttgagtcag attattccat tctatttctt ccataatcat
     8341 catgtccttt tacaattcat atacttattt tatcgtatat atttaaaaag gaaaaggaca
     8401 ctgtttaaat agtaaacgat tcttaattaa gtttacttct attttcttga aattgaataa
     8461 ttttttcaga aaatccattt gcaactttcc cctcatctcc aataaaatca agataagtaa
     8521 aatctgtccc aagtgaatta ttaactattt tatcaatgaa aaatgaaatt atccctacac
     8581 ctaagatatc aactgtttcg tcattttcta ataaactatt tgttactgaa gcgattttat
     8641 ctgaagaacc aaatctgttc ttaacaaatt gattaaatct atcaatagca cccttatcac
     8701 aatagtacgt ttttaaagcc gataaagctg ttgaatcaga atttcttttt gctaaggtat
     8761 aaacatttac cgcgtcaaca tcttggtata aatcttctag actaaatgtt ccacctgcag
     8821 ccgtacctac taatgcaaat acaccttgct ccatttgtat tcctaatttc ttaacttcac
     8881 cccagcaagt cattaaatct cctaaccaac ctgcaaagtc agaaacacta tcattgattg
     8941 tgtcggctag aaacatctgt gattgtaacg tcacaattaa gtgaggcaaa cctattattg
     9001 aaccagtatt aggatctata aaataattat acaggccctc gctattagtt aatttattcc
     9061 tatattcatc ataaattttt ctatcatgct ctgttacagg agaagaaatg atgtcccatg
     9121 acaaaccaga ataatttaca tatctataaa atgtcaaatg agggttccaa ggattatcaa
     9181 gaccagcttg tgatagatat tgatacgcac gtaccatctg atttataacc ttctgattgt
     9241 atttggcaat gtaattatca ttattagtat catttgataa ctcattaaat ccgctatctc
     9301 tacccgatgt agcgattaaa tcgataccaa cagctccgtt gccacttcct atggtaaatt
     9361 caacaaattg atcaaaagac cattgtctag gcataggaaa acctaaattc ccactataac
     9421 cagtcgacat atctgaaaca aatgcaaacg aagcataccc gttattgatt atttgagaac
     9481 aaacatttct tgttccgtaa acacccgttt gatacccgct atatgacatt acagcagata
     9541 cccccttaaa atattctcca gcatatgcaa gaatttcttc ccctaacatg tctagatcaa
     9601 cagcaaaata aataactgtt gttaatggta tatttagatt ttttgctgca tctatagcct
     9661 tcttaccatc tgataagcca cgatcataag taaaataagc taatgctgca cctccatctt
     9721 gatagatagg aaacacagat aaatttttag aaaaaatatt attcaattcc tctgtagtta
     9781 gatacttatt tcgttcatct tttccaattc caacagtacc agtaagatat ctaccaacta
     9841 tttttatatc attatcaacc aatgtttgga tttgagatct agttaattgc gtagaagtat
     9901 ctgcgccatc tgcagatctt ctaatatctc cagcgctaga caatagtcct tttattacaa
     9961 caacatctgc tatttcagta tattcaggca ataccataaa ttttcggaat gccagtactt
    10021 ctaaacccat atatttagtg aaaataccat cgaatgtacc gtcttcaaaa taaccattaa
    10081 catataatcc atattgaatt attcttacat agtttcccga gttaccttca gacacaggat
    10141 aagttttact agttgtccca ttaccataag accctgttgc ttcactggga gaatatccta
    10201 gttcagcttg taatgcaaaa attaatgctt gatttgaatt tctttgataa ataccatcac
    10261 aaggcattat tcccgtgtat tctaaataat ttacatttaa ccattgctgc atttttctaa
    10321 ttttactatc tccgccggga acaagaacaa aagcactcat atcaaataac gctttggccc
    10381 atttcgcatc caaaactcca gttacagcgc ttccattaaa ccctgcatca gtctgcaact
    10441 cttttacagc agtatctaaa tatggagtat ataccccatc gaagccacct ggattaatcc
    10501 ccttacacca aaaggcacct tgaataagaa atacaatatt tttaggtaga tttttgcccc
    10561 atttaggagt aacctgatta tcgtacattt tttcagtggt aggtccaaaa ttagctacta
    10621 gattagtaat accaagttct acttgtagtc cttcaataag tccatagatt gtaggccacc
    10681 ctgttcgccc attttctgga accttatcaa accctgtaac attactgtat ttcttgttta
    10741 gccatttctg agtacttaaa accatctcat ccataaaaat cccctgcttt cttgccgaac
    10801 atacgttcgt ttatgtgttg att
//
LOCUS       DACDOA010000088        10677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDOA010000088 DACDOA010000000
VERSION     DACDOA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.088"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IWI39_002164"
     CDS             33..656
                     /locus_tag="IWI39_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209227.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="IWI39_002165"
     CDS             1106..2155
                     /locus_tag="IWI39_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9209228.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="IWI39_002166"
     CDS             2152..3033
                     /locus_tag="IWI39_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209229.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="IWI39_002167"
     CDS             3036..3806
                     /locus_tag="IWI39_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9209230.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="IWI39_002168"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="IWI39_002168"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9209231.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="IWI39_002169"
     CDS             5117..6280
                     /locus_tag="IWI39_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9209232.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="IWI39_002170"
     CDS             6397..6807
                     /locus_tag="IWI39_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9209233.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="IWI39_002171"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="IWI39_002171"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9209234.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9347..9664
                     /locus_tag="IWI39_002172"
     CDS             9347..9664
                     /locus_tag="IWI39_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209235.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9824..10060
                     /locus_tag="IWI39_002173"
     CDS             9824..10060
                     /locus_tag="IWI39_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9209236.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="IWI39_002174"
     CDS             10057..10455
                     /locus_tag="IWI39_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9209237.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3731 a         1774 c         2254 g         2918 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatat
//
LOCUS       DACDOA010000089        10477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDOA010000089 DACDOA010000000
VERSION     DACDOA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.089"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(115..2235)
                     /locus_tag="IWI39_002175"
     CDS             complement(115..2235)
                     /locus_tag="IWI39_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9209238.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2465..3643
                     /locus_tag="IWI39_002176"
     CDS             2465..3643
                     /locus_tag="IWI39_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9209239.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3659..4417
                     /gene="aroD"
                     /locus_tag="IWI39_002177"
     CDS             3659..4417
                     /gene="aroD"
                     /locus_tag="IWI39_002177"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9209240.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4440..4925
                     /gene="ybaK"
                     /locus_tag="IWI39_002178"
     CDS             4440..4925
                     /gene="ybaK"
                     /locus_tag="IWI39_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9209241.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4996..6249)
                     /locus_tag="IWI39_002179"
     CDS             complement(4996..6249)
                     /locus_tag="IWI39_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9209242.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6366..7715
                     /gene="gdhA"
                     /locus_tag="IWI39_002180"
     CDS             6366..7715
                     /gene="gdhA"
                     /locus_tag="IWI39_002180"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9209243.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7827..9173
                     /locus_tag="IWI39_002181"
     CDS             7827..9173
                     /locus_tag="IWI39_002181"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9209244.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9481..9867)
                     /locus_tag="IWI39_002182"
     CDS             complement(9481..9867)
                     /locus_tag="IWI39_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9209245.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9916..10092)
                     /locus_tag="IWI39_002183"
                     /pseudo
     CDS             complement(9916..10092)
                     /locus_tag="IWI39_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(10091..>10477)
                     /locus_tag="IWI39_002184"
     CDS             complement(10091..>10477)
                     /locus_tag="IWI39_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209246.1"
                     /translation="PLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKR
                     KQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFC
                     SKIRNEVDKKLFQARVTSLEQLLSTV"
BASE COUNT         3315 a         1926 c         2121 g         3115 t
ORIGIN      
        1 aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta caacccatca
       61 gtccttattg acaaagagcc taattttgtc gctgctcctt ttaaatttat tttatcattc
      121 ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac
      181 agttgattgt ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc
      241 agcttctttt aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc
      301 taatccacta ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc
      361 taactgttta tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg
      421 ttcagtaata gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt
      481 tggtgtaaca aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag
      541 atcttgtcct aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg
      601 caaggcatca acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat
      661 atggatcata taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact
      721 tgtttttcca actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc
      781 accgtaaagg acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa
      841 ttcttgtacg gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc
      901 atttgtattg gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg
      961 gttagataca gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac
     1021 ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac
     1081 atcataataa gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg
     1141 attccagaat gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact
     1201 taaaatatca ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc
     1261 ttgattcaag ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc
     1321 ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt
     1381 gatgaaaggt gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag
     1441 gaattgctgg aagctttcta aatggatata aatcacattt tttcctctag cgagcccgaa
     1501 caaatcgtca tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc
     1561 attgggactt gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac
     1621 catgaaagca ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag
     1681 caattctgga cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa
     1741 tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt
     1801 tgcaatcagc agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg
     1861 aaacagtcga atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt
     1921 gttgatcgta atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa
     1981 tgtcaaaaga aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata
     2041 aagagcaatt ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta
     2101 ttgaatgcca ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa
     2161 ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt
     2221 ttttatacat ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcctgaa
     2281 accatgttac acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa
     2341 ttgaaaccag aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga
     2401 gcagtaagac aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga
     2461 aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact
     2521 gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt
     2581 tgattcgcaa aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg
     2641 ttgggtattg agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt
     2701 gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat
     2761 attcaatgga gaaggagacg ggatcggcgg tctgattgtt gattattatg caggttatgc
     2821 tgttttttct tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt
     2881 tcgtgaaatc tacccggaaa tcataggagg ctatgagaaa attcgatttg aatcagtaaa
     2941 tctgcctgag agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga
     3001 aaacggagta aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga
     3061 ccaaaaagaa gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa
     3121 catgtttagt tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac
     3181 aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa
     3241 tggtttggat gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata
     3301 cgccaaaaga aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag
     3361 aaataagaaa aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct
     3421 ggatatttta gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat
     3481 agaacaattc caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca
     3541 acttgcttta tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa
     3601 ttatctgaaa gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat
     3661 ggttcgggta aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat
     3721 cggggccgtc cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga
     3781 tttagtcgaa tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga
     3841 aacattggtt cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac
     3901 cttagctgaa ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca
     3961 tgtgatacac acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct
     4021 aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca
     4081 tgattttaaa gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca
     4141 ttttggagcc gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg
     4201 gttgatggag ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc
     4261 catgggaaat cttggtaaaa tttctcgtct ggttggcgga acgacgggtt ccgttatgac
     4321 atttggttcc ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt
     4381 gaagcaaatt attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt
     4441 tggcaaagaa aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc
     4501 cctatacaga gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg
     4561 aacagcttcc agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga
     4621 cggggcctct tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa
     4681 aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg
     4741 gatacatacg tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag
     4801 atcaaattgc agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga
     4861 tggagttagc acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac
     4921 aataaatcat acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc
     4981 tttctttttt atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc
     5041 gaaagccgct gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc
     5101 aaaatacaga ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga
     5161 aacccctccg ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt
     5221 aaactctttg catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt
     5281 cagatgatcc atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc
     5341 atgccaagca ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat
     5401 aagctcttgt ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt
     5461 tttctctaat tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc
     5521 gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa
     5581 aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg
     5641 ttcatccgat atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg
     5701 cttcacgatc ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt
     5761 tgttaagata atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt
     5821 atgttctttg tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc
     5881 tttcagacga ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat
     5941 ccgtccatga tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc
     6001 gaaattattc cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc
     6061 aatcaaatca tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag
     6121 tagttttttt cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc
     6181 tgcactgatc gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag
     6241 tttttccatt ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa
     6301 aatgttttga gccaaaagag aaaatgagct aaaatgaggg aagataagca aaggagcgtg
     6361 gaacgatgac aaacgcaaca gaatatgttc aagcaattca agaaaagcta catcaaaagg
     6421 atcaagggca agaagaattt ttacaagcaa tcgatgaatt catgcccacg gttatgtctt
     6481 ttttggacac tcatcccgaa tatatcgaaa agaatatctt agaactttta acggaaccag
     6541 aaagagttat tcaatttcga gtgccttggc aagatgatga aggaaactgg cgagtaaata
     6601 gagggtaccg tatccaatac aactcagcta tcggccctta taaaggtgga ttgcgcttcc
     6661 atcctagtgt caatctaagt atcttgaaat ttttggcttt tgaacagatt ttcaagaata
     6721 gtttaaccgg tctgccaatc ggtggaggaa aaggcggaag tgacttcgat cctaaaggaa
     6781 aatctgataa tgagatcatg cgtttttgtc aaagtttcat gctggaattg tcaaaacata
     6841 tcggcccatc gattgatgtt cctgcgggag atatcggtgt aggagctaga gaaatcggct
     6901 atttgttcgg cgaatataaa cgattgaaaa aatatgatac tggtgtgttg acaggtaaac
     6961 ctttggattt ttggggaagt aagatacgta cggaagctac tggttatgga ttagtgtatt
     7021 atgtcaaaca tctattgaac gaagaaaaag attcatttaa tcaaaaaaca gtttttgttt
     7081 ccggaagcgg gaatgtagcc atttatgcaa tcgaaaaagt gcaagagcta ggcgggaaag
     7141 ttgttacatg ttcagattct agcgggtaca tctatgatcc agaaggaata gatgtgaaac
     7201 tattgaaaga agtcaaagaa atcaatagac aacgtttgac ggaatacgca aaccaacgtc
     7261 cttctgctat ttatcatgct ggagaatctg tttggacatt gaaagaaaaa gcggatatcg
     7321 ctttgccatg tgctactcaa aatgaaatag acgaaagctt ggccgaaatc ttagtagaaa
     7381 atgggatcaa aatcgtagca gaaggcgcta atatgccatg tacagtaaaa gctgtttctg
     7441 ttcttcatga agcaggcgtg tggtattgtc caggaaaagc tgctaatgct ggaggagtag
     7501 cagtatctgc tttagaaatg agtcagaacg tccagcgatt gacttgggaa agagagcaag
     7561 tggatgagca gctggatcaa atcatggaaa cgatttacca tacgtgtcgc caaacagcag
     7621 ccgagtacgg cgacgaaaaa aatctgcttc ttggagcaaa tgttgctgga tttgaaaaag
     7681 ttgccaaagc aatggcagca gaaggtttag tttgaaaaat gaaactttct tggaaatatt
     7741 gtcagcattt tgtttatttg cttgaaaaat aataaaaaaa aacgtaaagt attacctgta
     7801 gagtaaacct ataaggagga gaacaaatgg cacacattca ttttgactat tcaaaagtag
     7861 caccgtttgt caacgaacaa gagttagggt acatgcaaag ccaagtaaca gcagcccaca
     7921 acgaattacg ggaaggtact ggcgcaggaa gcgacttccg cggctggatc gatttaccaa
     7981 cgaactatga caaagaagaa tttgcacgta tcaaagaagc agctaaaaaa atccaatctg
     8041 attctgaagt attagttgtt atcggtatcg gtggttctta tttaggcgct cgtgcagcga
     8101 tcgacttctt gaatcacaca ttcttcaatt tattagatag caacacaaga aaagcaccac
     8161 aaatcttctt tgccggaaat tcaatcagct caacatatat tgctgacttg atcgaagtaa
     8221 tcggtgatcg cgacttttca gtaaacgtta tttcaaaatc tggaacaact actgaaccag
     8281 cgattgcgtt ccgtgtattc aaagagcttc ttgttaagaa atatggacaa gaagaagcaa
     8341 acaaacgtat ctacgctaca acagataaag caaaaggtgc agttaaagta gaagcagatg
     8401 cagaaggttg ggaaactttc gtgattcctg atgacatcgg cggacgtttc tctgtattaa
     8461 ctgctgttgg tttgttacca atcgctgtta gtggtgcaga tatcgatgca ttgatgcaag
     8521 gtgccgctga tgcaagcaaa gcatactcaa gcgacaaact agaagaaaac gaagcttacc
     8581 aatacgcagc aatgcgcaac attctttacc gtaaaggcaa agtaactgag ttgttgatca
     8641 actatgaacc aggaatgcaa tacttctcag aatggtggaa acaattgtat ggtgaatctg
     8701 aaggaaaaga ccaaaaaggc atctatcctt caagcgcaaa cttctcaact gatttgcact
     8761 ctttaggaca attcatccaa gaaggacgtc gcaatatttt tgaaacagtt gtgaaggttg
     8821 aaaaacctcg taaatcaatc acaattcctg aacaagcaga agatttagac ggtctaggct
     8881 acttgcaagg aaaagaagtg gacttcgtaa atacaaaagc atttgaagga acattgcttg
     8941 ctcatacaga cggtgacgtg cctaacttgc tagtcaaaat tccagaaatg gatgcttata
     9001 cattgggtta tacaatgtac ttctttgaaa ttgctgtagg tatttcaggt tacttgaatg
     9061 gaatcaaccc attcgaccaa ccaggtgtag aagcttacaa gaaaaacatg ttcgcacttc
     9121 taggcaaacc aggctttgaa gatcttgcta aagaattgaa cgaacgcctg taagataagg
     9181 gtttttcgct agatttaaat aatcagtgat agaaacatgt tcgctttaaa atgaaaactg
     9241 gtacccattt aggtactcaa acatactgca tcgtttattc agaataaggg gctgtgacaa
     9301 gacgtttgtc acagtccctt gtttgtatag aaatcgattg attgccttgc tctttagttt
     9361 gttttaggac tttaagaaca gtgaggctga cttcgaaggt taattctgtt tttttgtttt
     9421 ctagaatcag taagcaaaaa agcactcact tcgagtgctt ttttgcgata tattcttagc
     9481 ttaagatagg cctaattttt cttttacaga tgagggtacc ttatcgctgg taattacttt
     9541 tttctctatg atccgctttt cgcttgcttt caactgcaag atcgttcctt cggggtaagc
     9601 agaaccattg gcagagtcac cagaagtaat gacatcgaaa tctaactgtc ttttttcacc
     9661 gttttcagca taagcatcta cgttgtattt aaaacctctt ccaattactt ccccattatc
     9721 tgctttgata tcagtttctg caggcattgg agctttgact accgcataat aatctttacc
     9781 tacataagta gactgatagt agtcccaacc tttccatcct ataaaagcaa tcaatatgat
     9841 aacgatcaac cctaaaattt ttttcatgaa agatctcctt ttttattttt attatacccg
     9901 tggtgctagt taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg
     9961 tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc
    10021 tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag aaaaaacagt
    10081 ctcaactgtt tcagactgta gacaaaagct gttccaaact cgtaactcga gcttggaaca
    10141 gctttttatc tacttcgttt cgaatttttg aacaaaaaaa taaaggctcc ttgcctttcc
    10201 atagccaggt ggctaatttc tttaaattca ttgcagcaaa cactagcatc gtatgcgtcg
    10261 cgactttttc caagcctcga tacttggtcc aacgcatccc atgcttttct tttgcatccg
    10321 caaatattcg ttcaatcgtt tgtttccgtt ttttgtaaat tgatttattc agatctgtta
    10381 ggcgaagatg ttccacttca tccatcagat ctccccaaac atgtctcgtg atgactcgtt
    10441 gatgattttt acttttggta cattggttta gcagagg
//
LOCUS       DACDOA010000090        10247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDOA010000090 DACDOA010000000
VERSION     DACDOA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.090"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..494
                     /locus_tag="IWI39_002185"
     CDS             12..494
                     /locus_tag="IWI39_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9209247.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            531..1541
                     /locus_tag="IWI39_002186"
     CDS             531..1541
                     /locus_tag="IWI39_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9209248.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1656..2369)
                     /locus_tag="IWI39_002187"
     CDS             complement(1656..2369)
                     /locus_tag="IWI39_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209249.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2559..3149)
                     /locus_tag="IWI39_002188"
     CDS             complement(2559..3149)
                     /locus_tag="IWI39_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9209250.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3633..3860
                     /locus_tag="IWI39_002189"
     CDS             3633..3860
                     /locus_tag="IWI39_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9209251.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3876..5042
                     /locus_tag="IWI39_002190"
     CDS             3876..5042
                     /locus_tag="IWI39_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9209252.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5276..6319
                     /gene="hrcA"
                     /locus_tag="IWI39_002191"
     CDS             5276..6319
                     /gene="hrcA"
                     /locus_tag="IWI39_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9209253.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6413..6976
                     /gene="grpE"
                     /locus_tag="IWI39_002192"
     CDS             6413..6976
                     /gene="grpE"
                     /locus_tag="IWI39_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9209254.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7027..8856
                     /gene="dnaK"
                     /locus_tag="IWI39_002193"
     CDS             7027..8856
                     /gene="dnaK"
                     /locus_tag="IWI39_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9209255.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9007..10173
                     /gene="dnaJ"
                     /locus_tag="IWI39_002194"
     CDS             9007..10173
                     /gene="dnaJ"
                     /locus_tag="IWI39_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9209256.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3494 a         1745 c         2188 g         2820 t
ORIGIN      
        1 gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt
       61 tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaagaacaag
      121 gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat aataactacg
      181 tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag atcgtcggta
      241 aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa aagcagggcg
      301 ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa gagctcttat
      361 caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc atccaaacgg
      421 ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg ccggcaaacg
      481 aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac atgataagtt
      541 atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca ggcgtatcag
      601 aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa tctttccaag
      661 tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac
      721 gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc tttccgactt
      781 ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct ctacaaccaa
      841 aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca ttaaaaacag
      901 ggatagccac tgcacatgga actaacctta ttgatttaag aggagagtac ttggatgaaa
      961 cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt ctacagcaat
     1021 cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc atcatcttct gttttccatc
     1081 tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg aagggacgat
     1141 tattaggtgg ttgtattgat acgatcaggc atttaatcgg tactccttat ggagatgtcc
     1201 aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta gaaaactgtg
     1261 agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca ggctggtttg
     1321 atggcagctc ggggatcatg ttcggtagaa gtgcggcaga ggatttgtta gattataaaa
     1381 tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg tatgatattg
     1441 attgtggtca tgtaccgcca caactgacat tgattaatgg tgcctatgca gaagtagcgg
     1501 tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt tcctgccttg
     1561 gagtctatca aagaccaata aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg
     1621 ctgtcagctt ctttttgtat aacatattag aaagtctaat tatctataac tgtcacatcg
     1681 attaactgtt tcacgtcatt gggatttcct gtaggaatag ctactacaga tgcattatca
     1741 atgatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc tgcaggcatt
     1801 tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact tgaatacact
     1861 aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg atagactgca
     1921 ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac atgtaattcg
     1981 aaatcacccg atgtatctct aaaagtatta cctctaatag ctagccaaac acgtgcgtac
     2041 tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga ctgtgaggag
     2101 gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga tgtagaagaa
     2161 gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt agaagctgat
     2221 ttctgagtac ttgatttttc agctgaactg gtttgtctga ttgaactttt gtctgtggtc
     2281 gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa taaagagacc
     2341 aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt ttaataccag
     2401 tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg
     2461 tatcttcttt gataaatgtc agaaaaagtg tgacaaataa tcatcagtaa aattacttgt
     2521 cacactattg ataaggagta tttaattgaa gagaatgatt aggagaaagg atctccgtac
     2581 tttttatagt aatcatagaa tccaagtttg tatagacgaa tcgttttttc tcttatttct
     2641 tctgtttcaa aatggactgc ttgaagaatc aagtctgtga attctatagg agctgttcca
     2701 ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt ctataaaatc ttctttattc
     2761 tgataatttt ccgattctaa ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc
     2821 aataatccgc tctgagctaa ataatcaact gccccacaaa tagcagctac tggtacgtct
     2881 gtttttaaca atttatcgat tatttgatat aaatgatgat tttttatgtc ccatgaattg
     2941 ccaccgatca taataaaaag gtcaactttt tcaggtattt gttcgattaa gtaatcgatt
     3001 tttgttttta atcctccaat ggaagtgacc tctgattgga tagaagcagt tttgttttgc
     3061 catttctcat tttggtttag tgtgctagta atataagcac cttcccaatc ggcatattcc
     3121 tctaatagaa acgtaatcgc agttttcatt ttcaagctct cctttactat ctataacaag
     3181 gacaacagtt ttaacctaca gacattcatt aaaattgggt agcgacagcg aaaacaacaa
     3241 ttcttaagtt ttagacagag tatacagata gtcaacttaa ttaaatacga actaaataaa
     3301 aatgacaggt aatcattttt gtttaccagc attataacat tttgcataca tggatgacaa
     3361 aagaaccaat tcccacttgc ttgagtacaa tattaaaaga aactggaagg caaaaagtaa
     3421 ttatagaaac gaaaaataaa tcatatcggg cactcagaca tgaaaacaac cctagagata
     3481 tataactagg taaccagttt aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt
     3541 ttttagtgga tttgttcgcc aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa
     3601 gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat agcttttcat atttttgcag
     3661 attttgactt aatgccgaag gtcagctttg cgattaccgt ttatcgagag gctgtaaaag
     3721 caccgtgtag ctccgatgac aataagcaag catttttaga gctaggtttc cttgattcta
     3781 aatatgcggc ttatttcaat gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca
     3841 ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata aatcccgcag
     3901 aaaacaaggt ttctgcgtac attcatatcc cattttgcga gcatatctgc tattactgtg
     3961 attttaataa agttttttta gaagggcagc cagtagatga gtatgtggaa atgctgctga
     4021 atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag
     4081 ggggcgggac accaacttct ttatctgcta aacaacttga tcgactgtta tcaggtgcta
     4141 gagagatcct gccttttcaa aatggtaatg agtttactgt agaagcgaat ccaggtgatt
     4201 tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt caatcgttta tccatgggag
     4261 tgcagacatt tgataatcgc ttattgaaaa aaattggacg taaacacacc gcagaagatg
     4321 tgtatcaaac tatgcaattt ttagaagcag aaaattttac caatgtaagc atcgatttga
     4381 tttatgcatt accgggacaa acactagaag gatatctgga tactttggaa aaagctttgg
     4441 ctttggatct gcctcactat tctttgtact cattgatcct cgaaaacaaa acaatgttca
     4501 tgaactgggt acgacaagga agactcgaac tgcctgatca ggaaacggaa accagaatgt
     4561 ttgaagagac gattgaagca atggaaaagc atggacgtca ccaatatgaa ataagtaact
     4621 ttgggctctc cggacatgaa tctcaacata acctcatgta ctggaacaat gcccactatt
     4681 ttgggtttgg agctggtgct agcggttatc ttggagatat tcgttaccgc aacaaaggac
     4741 caatccaaca ctatttacgc ccattacgag caaatgaacg cccggtattg gaagaagaaa
     4801 aattgacacg aaaaaatcaa atagaagaag aaatgttctt aggactaaga aaaaaaacag
     4861 ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg
     4921 atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt
     4981 tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg tttgaaaaat
     5041 agtttaaaga atatttgttt tttatctgaa tttaaataaa aaaagttcaa aaatacaata
     5101 aaggactatt gaaacgttga tataactgga tttacatcaa ttatttagaa ttagcactct
     5161 tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca ggagaagggc ttcttgctat
     5221 atttataagt ggattagcac ttagaatata tgagtgctaa tgagaggtga ggaagatgtt
     5281 aacaaagcga caaaacgaca ttttacgtct gatcatccaa cactatacga gtagtggcgt
     5341 gcccgttggt tccaaaacat tgatgtctga gggagtcaag gccagctcag caacgattcg
     5401 caatgatatg aaagcattag aagatgaagg attgctgttg aagactcact cttcttctgg
     5461 gcggattcca tctgctttag gctatcgtta ttacgtggat catttgttaa aacctgctcg
     5521 cgtagcagaa gatgatttgc tgctgattcg tcagtctcta ggcagagaat ttcatgaaat
     5581 caatgagatt attaagcaat ctgctgagat attatctgag ctgacaagct atacgacttt
     5641 ttcattggga cctgaaataa aagaccgccg actgactggc tttcgtatcg tacccttgaa
     5701 taatcgacag attattgcta tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt
     5761 cactcttccc gaatatatgg gaagtcagga actagaaaaa atggtacaga ttgtaaacga
     5821 ccgtctagtc ggagatcctc tgatgacggt gtaccataaa cttcgtacgg agattccgat
     5881 gattcttcat aaatatttcc aaacgacaga agggatgtct cagttatttg acaccgttct
     5941 tggtcatgtt tttgaagata aggtttatgt cagtggacaa atgaatctct tggattatga
     6001 gccccgtcaa gatcttgatc agttcaaatc gatgttttct tttatgaaag actcagacgg
     6061 acttacacaa atgatcactc cgatggatag caatattcat atcaagattg gtgctgaatt
     6121 aggcaatgat ttattgcaga atatgagtat gattcaagca agttacgaga tagatggtca
     6181 tggaagaggt acaatagcct tgttagggcc tactagtatg ccttattctc ggatgttcgg
     6241 tctagtcgat gtttatcgaa gagaactagc agctaaactt gctgattact accgttcgct
     6301 tgacttgtca aactcgtaat gtgaataacg aagctatttg cagctgatcg ttctctcttt
     6361 cttagtaaga gcaatcaaac agctgactga ataatgaaag gaagactgta tcgtgaaaga
     6421 aaatcaagaa gaactagata aagaaatgac agatgctcag ccagaaccgg aaatcgatgt
     6481 cgaagctgca gaagattcgg gtatttcaga agctgaagcg gaagaattcg aaacagcgaa
     6541 attaaaagct gagttagaag aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc
     6601 aaatatggca aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc gttcacaaga
     6661 tttagccaaa aaattacttc catcaattga caacctggaa cgtgcattag ccacagaagt
     6721 gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg
     6781 taacgcactg gaagaagaag gaattgaaaa aatccctgct aaaggagaag cctttgatcc
     6841 taatcttcat caagcagtac aaactgttcc agcgactgaa gatacaccag ccgatacgat
     6901 tgtagaagta ctacaagaag gctacaaatt acatgatcgc gtgttaagac caaccatggt
     6961 cattgttgca caataattgt gcggatataa aacttacact ttcaattaaa gaaggagacg
     7021 ttaattatga gtaaaattat cggtattgac ttaggtacga ctaactctgc agtagcagta
     7081 ttggaaggaa acgaagcaaa aatcatcgct aacccagaag gaaaccgtac aacaccttct
     7141 gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg
     7201 acaaacccaa atacgatttc atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta
     7261 gatgtagaag gtaaatcata tacaccacaa gaaatctcag caatgatcct tcaatatatc
     7321 aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca
     7381 gcttacttta atgatgcaca acgtcaagca acaaaagatg ctggtaagat cgctggtcta
     7441 gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg tttagataaa
     7501 acagatcgcg acgaaaaaat cttagtattc gaccttggtg gcggtacttt tgacgtatct
     7561 atccttgaac taggagacgg tgtctttgat gtattgtcaa cagctggcga caaccatcta
     7621 ggtggggacg actttgataa caaaatcatc gactacatgg tagcagaatt caaaaaagaa
     7681 aacagcattg acttgtctca agacaaaatg gctttacaac gcttgaaaga cgctgctgaa
     7741 aaagcgaaaa aagatttatc tggcgtatca agcacacaaa tcagcttgcc atttatcaca
     7801 gcaggagaat ctggaccatt gcatttggaa atgacattga cacgtgcaaa atttgatgaa
     7861 ttaacagctg acttagtaga acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca
     7921 ggtttatcac aatcagaaat cgacgaagtt attttagtcg gtggatctac tcgtatccct
     7981 gcagttgtag aagcagtacg taaagaaaca ggaaaagaac caaacaaatc agttaaccct
     8041 gatgaagtag ttgctatggg tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa
     8101 gatgttgttt tacttgacgt tacaccattg tcattaggta tcgaaacaat gggtggagtg
     8161 ttcactaaat tgattgaccg caatacaacg attccaacaa gtaaatcaca agtattctca
     8221 actgcggcag ataatcaacc agcagtagac atccatgtat tacaaggtga acgcccaatg
     8281 gctgctgaca acaaaactct aggaagattc caattgacag atattccagc agctcctcgt
     8341 ggtattccac aaatcgaagt aacattcgat attgataaaa acgggattgt aaatgtatct
     8401 gcgaaagatc taggtacaca aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta
     8461 acagacgaag aaatcgaacg tatggtaaaa gatgcagaag caaatgcaga agcagataaa
     8521 gctcgtaaag aggaagttga cctacgtaat gatgtagatg cattgctatt ctctgtggac
     8581 aaaacattga aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca
     8641 gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa
     8701 cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta aactatacga acaagcggca
     8761 caacaacaag ctcaagaaaa cccagaagca gctcaaggcg gagcagatga tgtcgttgat
     8821 gctgattttg aagaagtcga tggcgacgat aaataatcag ttcactgaat ttcgagagaa
     8881 ggccaaagtt gctactttgg ctttttcttt tgaaaaatag tttgaagtat cggtttttgt
     8941 ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat gatgagaacc aataggagga
     9001 aagcctatgg caacaaaacg tgattattat gaagtcttag ggctctcaaa aggagcatca
     9061 gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat
     9121 aaagaacctg atgcggaaga aaaattcaaa gaagtatcag aagcatatga gattttaagt
     9181 gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg caggcacaga tccaaattac
     9241 ggtgctggtg gagctggtgg cggatttggt ggattcggcg gcttctctgg tggcggcttc
     9301 ggtggttttg aagatatctt cgactctttc tttggaggag gcggtcggtc agtcgatcca
     9361 aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg
     9421 atttttggtg tagaaaaaga aatcaagtat aatcgggaag aaatctgtca tacttgtggt
     9481 gggaacggtg caaaaccagg cacacaacca actacttgtc ataaatgtca tggtgcaggt
     9541 acgattaatg tagaacgcca aacaccactt ggacgagtga tgagccgtca aacttgtgat
     9601 gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc caacctgtca tggctcagga
     9661 catgagaaga aagcacattc agttaaagtg aatgtaccag ctggtgtaga agaaggacaa
     9721 caaatgcgtt tagctggtca aggagaagct ggagaaaacg gcggaccatt tggtgatttg
     9781 tatgtggtct tccgtgtaga agaaagtgat atctttgatc gtgaaggttc tgaaatttac
     9841 tatgaattgc ctttaagttt tgtacaagca gctttggggg atgaagtcca agtaccaact
     9901 gtccatggag atgtgaaatt gaagatccct gctggaacac agacaggtac gaacttccgt
     9961 ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa
    10021 gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt acgtgcattc
    10081 gctgaagctg gcggcatcaa agttgaagaa cagcaagaag acggtttctt cgataagttt
    10141 aaagatgctt ttggcgggaa aaagcggaag tagtttttct agtaaaaacg ctaagtcatt
    10201 aataattaaa aaccagacga acaatcatat gttcgcctgg ttttttt
//
LOCUS       DACDOA010000091        10121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDOA010000091 DACDOA010000000
VERSION     DACDOA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.091"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..381)
                     /locus_tag="IWI39_002195"
                     /pseudo
     CDS             complement(16..381)
                     /locus_tag="IWI39_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(455..1105)
                     /locus_tag="IWI39_002196"
                     /pseudo
     CDS             complement(455..1105)
                     /locus_tag="IWI39_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1430..1846
                     /locus_tag="IWI39_002197"
     CDS             1430..1846
                     /locus_tag="IWI39_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9209257.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1843..2313
                     /locus_tag="IWI39_002198"
     CDS             1843..2313
                     /locus_tag="IWI39_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209258.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2327..3100
                     /locus_tag="IWI39_002199"
     CDS             2327..3100
                     /locus_tag="IWI39_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209259.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3114..3926
                     /locus_tag="IWI39_002200"
     CDS             3114..3926
                     /locus_tag="IWI39_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9209260.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3980..4945
                     /locus_tag="IWI39_002201"
     CDS             3980..4945
                     /locus_tag="IWI39_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209261.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5061..7784
                     /locus_tag="IWI39_002202"
     CDS             5061..7784
                     /locus_tag="IWI39_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209262.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7797..8429
                     /locus_tag="IWI39_002203"
     CDS             7797..8429
                     /locus_tag="IWI39_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9209263.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9328..>10121
                     /locus_tag="IWI39_002204"
     CDS             9328..>10121
                     /locus_tag="IWI39_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9209264.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3393 a         1570 c         1956 g         3202 t
ORIGIN      
        1 cggctcacca aactatttat ggagtcgttt taagaaagca taggctgctt gcgtatcccg
       61 tttctttcgt aaccagatat ctaaggttaa gccgtccgct tcaattgcac gataaagata
      121 atgccaacgt cccttaattt tgatataggt ttcgtccatt ttccatgaat agaaggattg
      181 tctatttttc ttcttccaaa gataatagag gactttgctg tactcttgta cccaacgata
      241 aatcgtagta tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata
      301 gcttagattg taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg
      361 tttgccttta aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta
      421 aaatagattt tttggaaaac tttgcaacag aaccgccaat catatggaag ccaccgatta
      481 ggtattttat gggtttagtc ggaaatgttt caagagcaat ttccatcata tcgataatgc
      541 catgatgact acagccagta aagacaatca gaccgtcttc ttgctcaatt acgaccaata
      601 attcatctga aaaatcatca taaacaaatt tatcatccat ctttttgtat aaattcttgg
      661 tggtcgagtt tgctttgcct agggggatga tatggaagct cggagcaagt tctgttttcg
      721 tttttattgt atgtaaattc tcatgcattt taaggatttc atttttagga ataccaacat
      781 acagaaacgt ggttaaaatc tttaaataat ggggtaaagt tacaccttca gctgtataaa
      841 taggggcgtt tcctgatttt aaatatctta acccgccggt gtggtcagaa tgaccgtgtg
      901 aaataatcac tgcctccaga tcattcaaag tttcccccat tttagtcgca ttatagacta
      961 attttttatt agcgccagca tcgaagagat atttcttccc tgagtagtta attaagagag
     1021 ataacccatg tccactttta aagttcaaat ttattcttga gttttcaaat aaaaccttga
     1081 tttccactat aacatctcct ttcacatgaa cgtgtattca tatattgtag cacattaagt
     1141 tctatttttt gttgtaatta ccgaaaaaaa tattaatgga cgctttttac gctttatttc
     1201 gtatgtggaa gaaaatttct taggatgtgg cgggggattg gggcaaagct aaattccaat
     1261 tacagtgatc atttaattag acacctttaa atacccacac tttcatcaac actataattt
     1321 agggaaagaa aagtgttggc atgatataga aaaaccttta attaacagcg tttcctaaaa
     1381 ttggcacgca tcttgcttat agaaaagcgt aaggatagga ggaatgaaaa tggcaactat
     1441 aattcttgcc agccatgggg aatttgctca aggattaaag caaacagcta caatgattat
     1501 tggagatagt gtaccaattc atgccttgtc agcatttaga gatgaagatg aatctatttt
     1561 ggtacaaata aaaaaacttt tagccacaat agatatagaa gaaacctaca ttttaactga
     1621 tattctaggt ggtagtgtga ataatgatat gctcactatt attaaagagt atgagggatt
     1681 gcaattaatt actggaatga acttaccttt agttatttct attgcaacct ataatggaaa
     1741 aattggggaa agtgagctag aaaagcttat taaagagagt caacaatcag tgattgattg
     1801 tagaaaatta ttagtagaac aacttactgg aggagatgac ctatgattaa gttagtacgt
     1861 attgaccatc gtttgttgca cggacaggta gtattttctt ggagtaaatc attaaatatc
     1921 aatcgtatta ttattgccaa taaagaggca gcgaatgatg aattcaaaaa aatgtctttg
     1981 aatttatcta aaccgactgg cattaaatta acaatttttt cagttgaaca gttactagaa
     2041 aaaatgccta aaatcgaaac acttaacgaa aatatcatgc ttatttttgg taatacaaaa
     2101 gaaattcgtc aattttgtga agcctatcct aaagtacaag aaatcaacta tggagggata
     2161 atcaaaaaag aaggttccaa gcaatttagt aatgccattt tcttgaatga atctgagcaa
     2221 gaagatgcca aaaaattaaa agctcttggc attaaacaat ttattcaaca ggtaccaact
     2281 tctaaaaaag aagatttgaa tacaatgtta taaaggagga atgaaaatgc tcgttcagtc
     2341 aattggttta gggttaatcg gtgttttttg tattttagac tcaaggcttt taggaagaat
     2401 gaattttgaa cgaccgttga ttgtcagtac gcttgttggt ttggtacttg gagacatgga
     2461 aaaaggatta attgttggag cctctttgga attgatgtcc ttaggattag taaatatcgg
     2521 agctgctgca ccacctgata tgaatatggc atcaattatt gctacagcct ttgccatttt
     2581 atcaaatgcg aatgcagaag cagcattaac gattgccatt ccaatttcag tgttaggtca
     2641 aatactaggt atcttaatgc ggacgatttt gtcaaatttg actcacgttg cggatcgctt
     2701 tatcgaacaa gggaaattta gccaagcaag gagaattcat atactttatg gctcgggatt
     2761 atatgctctc atgtatttta ttcctatttt tctagcaatt tattttggaa cgaatcttgt
     2821 ttctcagatt gttgatgcga ttcctgagtg gctaacaaat ggcttaacgg ttgcaagtaa
     2881 aattttacca gcatatgggt ttgcgttgtt attacaaacg atgttaacga aaaaaatggt
     2941 gccattttta ttacttggat ttttaattac tgcttattct ggattgggaa tcattggtat
     3001 tgcagccttc gcatgtattg ttgcgtttgt catggttcaa gttcaaggta aaaactcaaa
     3061 tggtaaccaa aatgagccag atccattaga tgacttataa ggaggtagat taagtgaaag
     3121 agaaagatca aaatgcatgg aaatatcggc agttcttatg gcgttcatgg gcaattcaag
     3181 cctcatggaa ctatgaacga caaatgaaca tgggttttat gtacggaatt gcaccaattc
     3241 tagataacat ttacaagaat ccagaagatg aagcaatgaa gaaagaagct tacgagcggc
     3301 atatgatgta ctacaactgt acccctcaaa caagtgcatt tgtgttaggg ttaactgctt
     3361 ctatggagga gcaatactac gaagaccaag aaaactttaa tccggaatca atcagtgcag
     3421 tgaagacttc tctgatggga ccgctatctg gtgttggtga ttctttcttt caaggaacga
     3481 ttcgggtatt agcctttgga ctaggaatta atttagccca acaaggtagt attttaggtc
     3541 caattctagc aatgttaatt tcctttgtac cttccttttt agttacttac tatggtggga
     3601 aaattggcta taacatgggg aataaatact tgtctaaact gtatcaagaa ggtttgatgg
     3661 ataaagtaat gtatgtttgc tccattgtgg ggttgatggt cattggttcg atgattgcca
     3721 gcatgatcgg gattacaaca ccaattcaaa tgggtaaagc ttttgtctta caagatgtat
     3781 tagatagtat tatgccacaa tttttaccat taggattaac cttcttcatg tactggttat
     3841 tgcagaaaaa ggtcaaaaca ggatggatgt tggcaatctg tttagtcgga ggaattattt
     3901 ttagtttgtt aggtatctta caataaggga ataaccaata aatttggttg aataatataa
     3961 ataaggagga attcaattga tgaattcaaa agaaattgtt aaagaaatcg tagcgaaaaa
     4021 agaggtaaaa tcaatcgtat ttgttggctg tggcgcttca aaagcggacc tttatcctgc
     4081 aaagtatttt ctagaccaaa atgcgaagca gttacggatt agtcattaca cagccagcga
     4141 atttaactat gcaacaccag catctgtaga tgagtcaact gtcgttattt cagcttcttt
     4201 aggaggtacg actccagaaa cagttgaagc taacgcagta gctaaagaaa aaggtgctac
     4261 agttattagt ctaacacgaa gcattgactc accgttaaca aaagatgcag attatgtaat
     4321 ttatcatcga tttgcagaga actatgctgc taaattagaa aaaacaggct actgtttaga
     4381 attggcagta gaacttttac atcaagtaga aggttatgac cattatgaag caatgcaaga
     4441 aggatttgct actatctatg atcttgccca aaaaagtgca gaatcagctc gtaaagatgc
     4501 gaagaaattt gctgaacgct ttaaagatga aaaagttatt tatgtaatga gtagtggtgc
     4561 tacacatgaa gtagcatact caacatcaat ttgcttgatg atggaaatgc aatggattaa
     4621 ttctggtacc ttccattcag gagaattttt ccacggacca ttcgaaattg tggataaaga
     4681 cgtaccgttt atattattaa tgaatgatgg aaaaactaga aatatagatg ctcgagcatt
     4741 aactttcctt gaacgctttg atacgaaaac agaagttgta gatgccttgg attatggctt
     4801 gtcttcacat attgctaaag aagtcttaga ctactttaac ccattcgtga ttactgcagt
     4861 atttagagtt tatgcagaag aattgtcaga agcaagagaa catccattaa ctaagagacg
     4921 ttatatgtgg aaactaactt attaaaaaat agatcaaact aaataaggtg gcttgagaaa
     4981 ttaagccacc ttgttttctt caatgtgatt ttatacatat ggtaaaataa ctagtacata
     5041 ttgtgaaagc aggtggcaag atgcagcgat taaatgacta tctttatcaa ctaacattgc
     5101 ttttgaaatc tacgccttca gataattatt cagctcgcag tatttcgcaa gctttacagt
     5161 tgaatagaag tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa
     5221 aagtaaaaag ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca
     5281 cactgaataa ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg
     5341 cattagatca agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa
     5401 cggcagtctt atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag
     5461 ggaaaacctt tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca
     5521 catcggcacc tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat
     5581 cctctgcttt atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg
     5641 gtttgctaga aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg
     5701 aagaagggca agagaaatta tttacattta tggatacggg tgaattctct cctatggggg
     5761 ataatagtat tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat
     5821 atacgacttt cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat
     5881 ttcaacaacg tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga
     5941 gtaaaatttt agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata
     6001 gtgattttga aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg
     6061 catatatcca ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag
     6121 agtacagtta taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa
     6181 tctactatcc agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc
     6241 aattttttga ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta
     6301 aactattttt agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc
     6361 aagaggatta cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa
     6421 gtattgattt tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt
     6481 tggccaacta tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa
     6541 agaaaaccaa agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt
     6601 atgcaaaaaa aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg
     6661 acattttctt tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta
     6721 ttaagagtat tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca
     6781 accgtatgct gaataagaac gtttttcaag cttatgatat gcctttagat attacattag
     6841 agaaaattga agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga
     6901 ttttattggt agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa
     6961 atggaccact gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac
     7021 aagtattaaa caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt
     7081 tcaaaaagca gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt
     7141 atacagggat tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg
     7201 cagttagaga attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt
     7261 ttgagactcc ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa
     7321 ttaatcagat tccatatatt ggattagatc gcctgattaa tggggaagca attggcgaat
     7381 ttgttcatta cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa
     7441 ttttcaattt ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg
     7501 tgctatcttt tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt
     7561 ctaataatca gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac
     7621 gtaaagaaag tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa
     7681 taatggataa aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac
     7741 cagatttaga gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga
     7801 caatgggagt aatttttgat atggatgggg tactcataga cagcgagaca ttttattttg
     7861 agagaagaat gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg
     7921 attatgtggg gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat
     7981 taagagagga actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg
     8041 ctaaagattt acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac
     8101 cattggcact ggcatcttct tctccagttg ttgagataga gagtatgctc attcaatgtg
     8161 aacttactga atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac
     8221 atcccgaaat atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag
     8281 aggattcaac gattggtatt aaatcagcaa aggcagcaaa tatctatacg gtggcactaa
     8341 aacaaccaat gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac
     8401 tggaacaaat tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt
     8461 gctagctatt ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg
     8521 gtaacacctt ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt
     8581 attttgttcg caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc
     8641 aacattctat ctatttcaga acgtaacctt ccccagttca ttgggattca ttagcataca
     8701 accaacattt caaaaagaaa ttgcactaca ccattattag ctattttgac taaccccaaa
     8761 aaacttgcta taattcaggt gtaggtaaaa ggtgctggta tcacctttta cgaaactgct
     8821 tcggcagtgg taatgagtgt ggcttaaagg actagtcctt taagcttttt actttgttcg
     8881 caatctctaa gatcaagtta atgatcgata agattgctag aatattttgc aacattctat
     8941 ttcagaacgt aacctttccc attccactgt gattcattgg caaccctcct cacaaccaac
     9001 gtttcaaaag aaattgtaaa gttgttgggg taccacgacc gaacaatact acacctgaat
     9061 gatagtaagt tgtctcatta agtatagcat atcctctttt tgggtaaata gaaatcggtt
     9121 acatttttta tccaagacca agttttttca tttggtctta tttgctatac ccaaacaatt
     9181 ccgcagtata gtcgacggta ttgattttat caggtgagta atagaaactt gttttccttc
     9241 cccaaaacat tacctataaa ggagaaaaaa tggtatcgta attatatagg tataggatga
     9301 aatttttttc taagaggagg gttgttgatg aaaaatttac agttaaaatt tattgttgat
     9361 ccatcgattg tacgctggtt tcggattatc aatcaatttg agcgcaatca acttgccaca
     9421 aaaggagaat tagcaaagag taatcatgta tctgaacgaa ccattcaaac agatgtcaat
     9481 aaaatgaagg actacttttc tgaaagtgtt caatttgttt caacgaaaag tgggtattct
     9541 tttcagaagg aaagaccggt tctttttctc aagcagaagg agcaactact ggagaacgaa
     9601 attttatttg agttgttagg gaatgtcttt tatggcgaat tggaagatat gacggagcta
     9661 attgatcgtt ttcatttatc tgaagcgact tttcgtcggt atttgaagca gattcaacca
     9721 gttttggaag aatacggctt agaattgagt ctttatcctc ttgatttaaa aggagaagag
     9781 gcaaatattc gcaagttttt taaagacttc tattatgaag gggagatgac gccccatact
     9841 cttgttccac caagggatct tcatgaactt gtgcaagcta cattctatga taaatttgag
     9901 tatttctcga ttggaacagg gacttctcct gctgaatttt attatagttt gtatattgcg
     9961 attgagcgtg tgagacaagg aaaaacgata gccattcctc aaggtctgat tgagcgagta
    10021 attgctagtg agaattttcg cttaatgtat tccttgaaag aagcgattca aacaaattat
    10081 caagttgctt tatcagaaga ggagttttgt tggttgtatt t
//
LOCUS       DACDOA010000092        10114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDOA010000092 DACDOA010000000
VERSION     DACDOA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.092"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..108
                     /locus_tag="IWI39_002205"
     CDS             <1..108
                     /locus_tag="IWI39_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9209265.1"
                     /translation="KEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            121..1020
                     /locus_tag="IWI39_002206"
     CDS             121..1020
                     /locus_tag="IWI39_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9209266.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            1023..1520
                     /gene="ssb"
                     /locus_tag="IWI39_002207"
     CDS             1023..1520
                     /gene="ssb"
                     /locus_tag="IWI39_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9209267.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            1886..2146
                     /locus_tag="IWI39_002208"
     CDS             1886..2146
                     /locus_tag="IWI39_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209268.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            2591..2797
                     /locus_tag="IWI39_002209"
     CDS             2591..2797
                     /locus_tag="IWI39_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9209269.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            2845..3048
                     /locus_tag="IWI39_002210"
     CDS             2845..3048
                     /locus_tag="IWI39_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209270.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            3064..3501
                     /locus_tag="IWI39_002211"
     CDS             3064..3501
                     /locus_tag="IWI39_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209271.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            3494..4201
                     /locus_tag="IWI39_002212"
     CDS             3494..4201
                     /locus_tag="IWI39_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209272.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            4581..4760
                     /locus_tag="IWI39_002213"
     CDS             4581..4760
                     /locus_tag="IWI39_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209273.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            5085..5348
                     /locus_tag="IWI39_002214"
     CDS             5085..5348
                     /locus_tag="IWI39_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9209274.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            5342..5692
                     /locus_tag="IWI39_002215"
     CDS             5342..5692
                     /locus_tag="IWI39_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9209275.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(5716..6330)
                     /locus_tag="IWI39_002216"
     CDS             complement(5716..6330)
                     /locus_tag="IWI39_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209276.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            6644..7066
                     /locus_tag="IWI39_002217"
     CDS             6644..7066
                     /locus_tag="IWI39_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209277.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            7076..7279
                     /locus_tag="IWI39_002218"
     CDS             7076..7279
                     /locus_tag="IWI39_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209278.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            7491..8075
                     /locus_tag="IWI39_002219"
     CDS             7491..8075
                     /locus_tag="IWI39_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209279.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            8446..9771
                     /locus_tag="IWI39_002220"
     CDS             8446..9771
                     /locus_tag="IWI39_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9209280.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            9764..10114
                     /locus_tag="IWI39_002221"
     CDS             9764..10114
                     /locus_tag="IWI39_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209281.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         3618 a         1576 c         1968 g         2952 t
ORIGIN      
        1 aaagagtaca tggaagaccc attgagagat ccaagtaaat taagagatta catgacaaaa
       61 ctacatgtag aaaaagaaat gaaataccac atgcaaagct tatattgatg gaggaaaaag
      121 atgacaccga cacaatttgc agcaaaaaca agaaatttag caaagaggac aggattaaat
      181 gcacagttag tacaacggca ttttatgatg gatcgtttaa ttgaacaaat ttctgaatct
      241 aagtataaaa atgatttcat tttgaaaggt ggctttttat taggatcaaa atatggaatc
      301 gatagaagga cgacaattga tatcgacacg acatttagaa atagcaaatt aactgaagaa
      361 aaattgaaga gtatttttaa tgatttaact aggaaaccca cgaaagaagg tattgttttc
      421 tctattcaag gaatgaagga aacaagagaa gccgattttt atcctggatt tcaaattaaa
      481 ttggtggcta atttagagaa agtacgtgtg ccatttaaaa tggacgtgac aacaggagat
      541 tctatttatc ctgaagtaga tacacactat caccaattaa tgtttgaaga taaaaaggtg
      601 gagattccag cttatccaac agcacaaatt attgcagaaa agctaagtgc tacgttcagc
      661 tttggcacag ataattcgag agcaaaagat tactacgact tatttaccat tccgaaacta
      721 gaaaatatcg atacgaaaga gttatacaaa agtgttcgaa atacatttac aaaacgtggg
      781 gatacaaaac cgttgtcctt ttattatgaa agagaaatgg ataagatcat aggaaatgac
      841 tatttggaaa agcagtggga aaaatatcgc tcagttaaca cgtttgcgaa ttccatttca
      901 tttacagata cagtcaacga aattgatcac ctaatgaaaa gtgtgatcaa aattgaaaat
      961 caagaaagaa aacggcagtt taaaagatca caacaaatga atcaagaaat ggagagataa
     1021 agatgatcaa taatgtaact ttagtaggac gcttaaccaa agatccagat ttacgttata
     1081 ccgcgagtgg tactgcagta gcgactttta ccctagctgt caaccgaaat ttcacgaatc
     1141 aaaacggcaa tcgagaagct gattttatta actgtgtgat ttggcgcaaa ccagcagaaa
     1201 caatggtgac attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc
     1261 ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg gccgataatt
     1321 tccaactatt agaaagcaaa gcagcgacag aaagccgtgc gcacgctgat caatcgtcca
     1381 ccagtccaag caccaccact tttgaacaac gggatacagc gacgcctaat aacaatggat
     1441 tgaacgcttt acagaatcca tttgggaatc catttggtgg acaatcaatc gatattagcg
     1501 atgatgatct accgttctaa gtagcatttt ctattggttg catttgctac ggtttatgtt
     1561 ataatttagt caacaaaaag aattttcaat ctataaccaa aaaccactag ctggcgggct
     1621 agtggttttc ttttacgttt ctattataag aattaacggg taggcttact gtctttcttg
     1681 gcaaatttac aatcaagcca tttgcagata tagtgcgcgg ctacttctgc catgattgca
     1741 aaaacaaaat caaaaagaat tttcaaacct ataacctcct ccccttgcca gattaggccg
     1801 gggtagcaga cagcaagact attatagcat aattgaaata tccaaatgat tggatttttg
     1861 ctataatttt agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg
     1921 agtaataaaa acgcaaccgt tattggttcg ctttacttta caaacgattc atgaatcgat
     1981 taattgtgta gttgcagata ttgaaattat cgataaattg ctaattatgg atgatggaaa
     2041 atacaatatt gccgtcacag gtcacttcaa taaacgaaat cagctagtta ttgctagcat
     2101 gtatgttaga aatcccgatc attttacgcg atcgatgggc atataaaaaa agaagagttg
     2161 caatcctaat gtaggactgc agctcttttt gttttacata gaaacaagaa aaatgaaaag
     2221 gcctttttag gaaggcgcga gcgccttctc aattgaaaaa cctctctcac tccaaccgcc
     2281 accgccgttc gagaagaaag gaagtgaacg ttgcacgttc actaagataa agggtcttgc
     2341 aaagcaagac aaaatagaaa aaacttcctc tcaccaaaag gttcgttcct gttcgttatc
     2401 tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga
     2461 caagggtaaa tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga ttcattctgg
     2521 ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc
     2581 agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg aattattccc
     2641 aaactatcat gcaccacata ctgtcgaatg cgattgtgga caaagagcga aatgtatcaa
     2701 aagctatcgt ttgtatcaat gtccaacatg cggaaaaaaa tacaccttgt cttatggcga
     2761 ttatattttg attgatgaga aaggaaagaa acgctgatga aaacataaca taaattattg
     2821 actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca
     2881 gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg
     2941 actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac
     3001 atggaaactt atgaaaacga atattggtta accaaattag agaactaaaa aggagaaaaa
     3061 aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     3121 aaaatggagg atatgacatt cctataccat gagacacgtg ttccaaaaaa acattataag
     3181 gaaagattgt cggtagctgt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
     3241 gcgacgtact atcgaacact ggaagaactg aaaaaacaaa atgcaaaatg gtttttccaa
     3301 gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc tgaatatcaa
     3361 gctttagaac taacgtatgg gaaatttgtc gaaacgaaaa aagccaagac tgtttcaagt
     3421 gaatggttag attattttga aaatatcaat aagtatggcg cctattacac catgaaaaag
     3481 gaggacaacg aaaatgaata aggtgattta tcaactaaca acggaagatg tgtcacactt
     3541 tttaacaaac aaagaacatt tgaatgaagt aatcagacgg attcaaaaag actttagttg
     3601 tatggaagaa atgaagtact ttattcaaag agtcgaagaa caattgtatt tggaaagtga
     3661 aacgagtcaa acgaaggtca cagtcagcaa taattattta gacgtgtata aacatcgtcc
     3721 tgaaacggta aaaaaaggaa cggagcttct acgactagaa acagaaaaat acatcatgaa
     3781 tgtttaccta gaaaggagtg aactggtcga aaactttgtc agctgcacga tttgcaggaa
     3841 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     3901 agaaatggaa gagctaaaca gcaaaaatgc gacgatagac gattattatc ttgtatttac
     3961 tagatttttg acgtgtggaa cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     4021 tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     4081 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     4141 aaatgaactt gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa aaaggcaata
     4201 atcatttata ccgaaaagtg agaatgtgtg aaataagaac aaaaaccttg aaattaaggg
     4261 aatggttctt atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat
     4321 aagcgggcaa agcccgataa cctaagagaa agaaggagat ccatatgaat aaaatcattc
     4381 ctctgttgtt cgtactgtgt attggcgaga tacttatgcg tcatggtgca gctgtacatt
     4441 taaacttcgt agtattctta ggaatactag tattttgttt gattttttgg cgctttgtgt
     4501 tagcccttgt gagtctacta ttcaagttga ttgcagccat atttttcata gggttatttc
     4561 gatgaaagga gagagtacta atgccaattg caataggaaa taaacggtta cccgtgacat
     4621 tagatgaaaa gagacaaaaa gaattgcagc aactaaagca gaagtacgga aaaagtgaat
     4681 ctaagattat gtgtattgcg ttagatctat tgatcgcgca agaaaaagca ggatttgata
     4741 taccagcact aaaaaagtga cgtcactttt catttgaaga aacaaaagtg atagcactta
     4801 attgttccta ctatttagaa tattttctat gtccttttta ttccattttg tatcaataac
     4861 tatgaaatct ttaaagtatc cagatcaatt tttttcttac ctgattctaa agattttgtt
     4921 ttcttaaaca tattatgatt tcctttcgac tattttcata taaatgttaa catagtttat
     4981 ggattcatgg aatgttaatc taatttaata gaaaaggata attaagtata tacgtttttt
     5041 cttatgtatg ttatactatg tatatacaga aagaggtgtc tactatggaa atgttaccta
     5101 taaaaaaatg gggaaatagt aatggcctta gattgccaaa gtatattatg gaatatcttg
     5161 gaattcatac agaagacaaa gtgaagataa tacaagaaga agtaaacggt cagaagcggc
     5221 taattatcga agcggctaat atagaaaatg aattaacaat tgaacagtta tttgaaaatt
     5281 acactgatga aagaaatcat gtgacgattc aaaatttagg tgaagcagta ggtaatgaaa
     5341 aatggtgaga aaacctagac aaggcgatat tctattattg aatacagcac ctcgatctgg
     5401 acacgaacag acaggtaaaa gaccttatat tgttttaagt catgatatta ttgcggatta
     5461 tagtaacgtt gttacagttg ctccaatttc tagtacaact agaaactatc cgctatatgt
     5521 aaatataaac tcagaatatg aaatgaagac aacaggaaaa gtattattag atcaactaac
     5581 gacaattgat tatgaagcca gagagtgtat ttttttggaa aaagcgcatg atagtttagt
     5641 tgaggagtta ttgatgaaag ttagaacagt ttttcaaaaa gtaagcaaat aaaatagaaa
     5701 cagcagcatg atcaactatt cgtgattgac catgctgtct ttttttattc tatatacggt
     5761 ttgtctcgta acattgtagt cttttgcaat cttagcgatc gcaacacctt tttttagatc
     5821 ctcaacgatg tttttataaa caagacgacg ttggggatct tttgcattgg cactgtatag
     5881 cttaggacgt cctttataaa caccgttcgc ttttgcaatc ttaatgcctt gtgcctgacg
     5941 tcgtttagat tcggtacgtt cttgttcagc gatcatggcc aaaatttgaa tgatcaaatc
     6001 tttaatgaat ttatccagga gaggattgcc aattgcttcg gccatgatcg gtagacttgt
     6061 gatgatcagc tgcacatttt tcttcttcaa gtaattgact gaagcgatga tctcgtcgta
     6121 atttcttcca agtcgatcaa tagcttccac aataaaaata tcttgatctc ggacgaaatc
     6181 cagtgcttct tgaaaaacag gtcgatgagt aatcgttgcg ccagattgtt tctcaatgaa
     6241 aattctctct gcaccgaatt ttttcatttc ttcaatctgc cgttgttcat tttgatcgat
     6301 ggatgagaca cgtatgtatg caactctcaa aatttttacc tactttctgc aactatttaa
     6361 aaaacaccct aatcatacac gagaaaacca gatttttcaa tgtcgcggtc gtgtctagtt
     6421 agggtatacc ttatatagac aaaaactaat gatggaggaa atagtatgtg tcacttttct
     6481 ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa acgattatga
     6541 cattaaaaat atattttatg aattttctag tatataactt gatttatctg ttaagattta
     6601 ttaagaaaac gttttcttat ttttgatttc gaggtgatag acaatgaaaa ataagtttat
     6661 tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt
     6721 tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat taactccttt
     6781 agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta
     6841 tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag tgacaggaac
     6901 gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga
     6961 aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata aatcagctgg
     7021 aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc
     7081 agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta aaattaattt
     7141 accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca
     7201 aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac
     7261 atatcaaaaa gaaaaataat gattttaaaa aaaagagtgt ctagtcgaca ttcttttttt
     7321 ctaattgaaa gtgttcataa aatcacccat ttctggacac ttcctttttt tgctttattt
     7381 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
     7441 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
     7501 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
     7561 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
     7621 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
     7681 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
     7741 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
     7801 agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac
     7861 gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa
     7921 aattgtctga acaagatttg gaattactga agactttagt ggatagcggt acacctatta
     7981 agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa
     8041 tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt
     8101 tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt gtctactgat
     8161 aacttattat caaatgttgt aaggatttta ttcatttgtt aatattaaag aacggaactc
     8221 caaaaaagtc cgtactctaa tttgcagtct ctcactcttt tagagtgaga gtttttatta
     8281 gattttcaaa ttaattagta aagtgataga ataaattgat catacaaatc ataagaagaa
     8341 gcagggtgat agcataccta tgcttctttt tttatgtatt tagctatttc ttctttacaa
     8401 catcgaacat atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     8461 aaagagccag taaatgatta ctttttaatc gatatgaaaa gcttttacgc aagtgtcgaa
     8521 tgtatagaaa gaaatttaga tccattaaca acagaacttg ttgttatgag tcgatctgat
     8581 aatactggtt caggattgat attagcttct tctcctgaag caaaaaagcg gtatggtatt
     8641 acaaatgtga gtagaccacg tgacttacca caaccatttc ctaaaacact acacgttgtt
     8701 ccaccacgta tgaacctata tatcaagcga aatatgcagg taaataatat tttcagaaga
     8761 tatgtggctg atgaagatct actgatttac tcgatcgatg aatcgatttt aaaagtgaca
     8821 aaatcactga atctttttac gactgaagaa acacgaagtc agcgaaggaa gaagctcgct
     8881 caaatgatcc aagaacgtat taaagaagag ttaggtttga ttgctacggt aggcgtcggc
     8941 gataatcccc ttttggctaa attagcctta gacaatgaag caaagcataa tgaaggattt
     9001 attgcggaat ggacgtacga aaacgttcct gagaaagtat ggaatattcc cgaaatgacg
     9061 gacttttggg gaattggttc tcgaatgaaa aaaaggctca accaaatggg aattttgagc
     9121 attagagacc ttgctaactg gaatccttat acgattaaga atagattagg tgtgattgga
     9181 ttacagctgt acttccatgc taacggaatc gatcgaacag atatagcaat cccgccagaa
     9241 ccgacaaagg aaaaatctta tggtaattca caagtgttac cacgagatta tactagaaga
     9301 aatgaaatcg agctggtggt aaaagaaatg gcggaacaag tagccattcg tatcaggcag
     9361 cataattgta aaacaggatg tgttcattta aacattggta cgtcgatctt agaaacgaga
     9421 ccaggatttt cacaccagat gaaaatacct atcacagata atacaaagga attgcagaat
     9481 tattgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     9541 acctatagca agcttgtgta caccgattcg cttcagctgg atttatttag tgatccacaa
     9601 aagcaaatta acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     9661 tttacgtcga ttgtacatgc ttcttcaatg ttagaaagcg ctagaagcat tactagaagc
     9721 acacttgttg gtggtcatgc gggcggaaat gggggaatta aaaatgattg attacaatga
     9781 tcgtggacgt ttaaagtggg cagggtttta tttatctgat catactgaag aaattgatgc
     9841 cgacaatgaa caacgaacca gtcaaaattt agcaaaagaa caaatgacga ctgaagagat
     9901 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatctattc aaaaagaaga
     9961 acgaaacgct gaaggatact atccagaaga tattgttggc aagatcttag gtagtgatga
    10021 gctggggatc tacgttggaa aagacaaaat tcactacgac gaaatacgtc atatcgaatt
    10081 ttaccaggat aataaatgga gtcgattaga atga
//
LOCUS       DACDOA010000093         9870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDOA010000093 DACDOA010000000
VERSION     DACDOA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.093"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..263)
                     /locus_tag="IWI39_002222"
     CDS             complement(18..263)
                     /locus_tag="IWI39_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209282.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="IWI39_002223"
     CDS             complement(378..956)
                     /locus_tag="IWI39_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9209283.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="IWI39_002224"
     CDS             complement(1033..1563)
                     /locus_tag="IWI39_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9209284.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="IWI39_002225"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="IWI39_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9209285.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="IWI39_002226"
     CDS             complement(2420..2815)
                     /locus_tag="IWI39_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9209286.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /locus_tag="IWI39_002227"
     CDS             complement(2812..4224)
                     /locus_tag="IWI39_002227"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9209287.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="cysE"
                     /locus_tag="IWI39_002228"
     CDS             complement(4221..4757)
                     /gene="cysE"
                     /locus_tag="IWI39_002228"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9209288.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5079..6539)
                     /locus_tag="IWI39_002229"
     CDS             complement(5079..6539)
                     /locus_tag="IWI39_002229"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9209289.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="IWI39_002230"
     CDS             complement(6709..7866)
                     /locus_tag="IWI39_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9209290.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="IWI39_002231"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="IWI39_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9209291.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="IWI39_002232"
     CDS             complement(9333..9860)
                     /locus_tag="IWI39_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9209292.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DACDOA010000094         9804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDOA010000094 DACDOA010000000
VERSION     DACDOA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.094"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            254..733
                     /locus_tag="IWI39_002233"
     CDS             254..733
                     /locus_tag="IWI39_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209293.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="IWI39_002234"
     CDS             766..2994
                     /locus_tag="IWI39_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9209294.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="IWI39_002235"
     CDS             3123..4766
                     /locus_tag="IWI39_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9209295.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="IWI39_002236"
     CDS             complement(4839..5384)
                     /locus_tag="IWI39_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9209296.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="IWI39_002237"
     CDS             complement(5407..5892)
                     /locus_tag="IWI39_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9209297.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="IWI39_002238"
     CDS             complement(5882..6694)
                     /locus_tag="IWI39_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9209298.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="IWI39_002239"
     CDS             complement(6833..8365)
                     /locus_tag="IWI39_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9209299.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="IWI39_002240"
     CDS             complement(8474..9505)
                     /locus_tag="IWI39_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209300.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3269 a         1827 c         1889 g         2819 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aatcattttt tcag
//
LOCUS       DACDOA010000095         9661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDOA010000095 DACDOA010000000
VERSION     DACDOA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.095"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            199..405
                     /locus_tag="IWI39_002241"
     CDS             199..405
                     /locus_tag="IWI39_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9209301.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(607..1608)
                     /locus_tag="IWI39_002242"
     CDS             complement(607..1608)
                     /locus_tag="IWI39_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9209302.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1613..3526)
                     /locus_tag="IWI39_002243"
     CDS             complement(1613..3526)
                     /locus_tag="IWI39_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9209303.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3694..4200)
                     /locus_tag="IWI39_002244"
     CDS             complement(3694..4200)
                     /locus_tag="IWI39_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9209304.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4360..4800
                     /locus_tag="IWI39_002245"
     CDS             4360..4800
                     /locus_tag="IWI39_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9209305.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4826..5983)
                     /locus_tag="IWI39_002246"
     CDS             complement(4826..5983)
                     /locus_tag="IWI39_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209306.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5986..6342)
                     /locus_tag="IWI39_002247"
     CDS             complement(5986..6342)
                     /locus_tag="IWI39_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209307.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6640..7614)
                     /gene="sgrA"
                     /locus_tag="IWI39_002248"
     CDS             complement(6640..7614)
                     /gene="sgrA"
                     /locus_tag="IWI39_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9209308.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7810..8649)
                     /locus_tag="IWI39_002249"
     CDS             complement(7810..8649)
                     /locus_tag="IWI39_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9209309.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8834..>9661
                     /locus_tag="IWI39_002250"
     CDS             8834..>9661
                     /locus_tag="IWI39_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9209310.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     "
BASE COUNT         2898 a         1949 c         1452 g         3362 t
ORIGIN      
        1 catattttct attattggtc tagacgtatg atggtaaact ttgtacctat ctgattgctg
       61 aatcattaat tgcttatcat tagccatctt tgtttcaagt tcgctagatg atcattttat
      121 tcacatccta acaaacttgt cacatttata gattcttaat ctatttatca tacaattata
      181 aaaaaggagg tgctgcttat gaaagctatc catatttctt tgcttttttt cgctagtgca
      241 ttgattttat ttgctattca tctttgttcc cagccttata tcaataacaa cggaatatta
      301 gtggaaccag cattcttttg cttaccactc ggctttttgt cccttcttgc ctccgcaggc
      361 ttcggatttg ttgctttttt taaacgatca aaacaacaaa tctgacttat aaaatttacg
      421 taaaagagat cgtgacaata atcgtgtgat attcccaaat ttcgaataaa aggtgcccct
      481 tgccatctct aactcctcag tcacaagtta caatattcat aaaccatgaa aagcttgttt
      541 attgaaattg ctccttgtga cttggagcta agtgacagat tacaacctct tttgcatcat
      601 aagactttac tccttgaaaa acattactaa cttttgatat gtttcttgcg cttgtttatt
      661 gctgaaatca aactgatatt catggttcaa aacagcattt ttctccgcat aatctacttt
      721 tgtgactttc acccccgctt tctcaaatac agaagttact tcttccattt gtttcgtaaa
      781 cgtatttgta tttccatccg ttaagaatgt gttttgcgtc caacgatccg catgtctgac
      841 caaggctaaa cttttgacct tctctgattg ttcaaagtcc ttattatcaa agtaagccca
      901 tccacatttt tcataaagaa agctagagat tttatcactt acttgagtaa gtgcagaagg
      961 atccaacaga ccagacaaag aaataaactt cgtaatctgc ttgttctcca aagtcggatc
     1021 aagatgatca gcttttttaa tttcttcatt ttgaagtgct aacaaatatt ctccactaat
     1081 ttgtgcacct gccgaatttc caccaatcga aatattcgac caatctgctt gatcttttat
     1141 ctctttttta gctgcttgaa tggcttcgtt caattggata actggtgtag gatgttttgc
     1201 ttccggtgat cgttcataat ctatatttac gatgatttgc tgcgtgtctg aacatagata
     1261 ttccaaataa tcacgacaat catttttatc ccctcctaca tatgcgcctc catgcatcca
     1321 gaaaagaatc ggtaattttt tcttttccac tttcggtgtg tagatatcca tattattatt
     1381 tttgtacttt gaaggatagg atatttcttt ttttatatcg atttgttcga ttttttgctg
     1441 gaaatcatct ggtcgggcat atacttgttc tttttcactt gtgtcaaatt gttttctaac
     1501 aagcaagcta atcggctttg gtgtgatttg attaatcaaa aatacagtga ctaaaataac
     1561 tacgatgata actggtactg caatcactat tcgcttgaac catttcattc atttaccctc
     1621 tctttcttcg ccaaatccat gtgataatcg tgctaacaga taaaaatcca cctgaataaa
     1681 tgagaagcga atgtttcttt tctccagttt tcggcaacat tctttttttt gcatctgtct
     1741 ttttttgtac tttcactgtc atctgaatcg gttgcgtatc atcacttgat tgatcctctc
     1801 gaggttcatt ctttccctta ttgtctgcat tctctgtcgg atcttgcttt tttggtggag
     1861 ctactgggat ttttttgtaa tagaaaataa tagttggtat cgtctcatta aaaatccctt
     1921 cagtcggtcc ttcaacattt gctaattcgt atccttcgaa tgtcttttgc tctgtcatat
     1981 aattttctcc ataagctccg taaagctgga tttcgtcctg gtctttgagt agacttttat
     2041 tgttttcatc cacatacttt acgtttatct gatcatggaa cactactttc aaaaagtccg
     2101 ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt
     2161 ctactaataa aagatcttta taccctcctg ataactcata taatttttgc gcgttagaca
     2221 caggtactac atcatctgct gttccatgga taatcaattt aggcaatgaa tctgacattc
     2281 caatcgtagg tagagcagct tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt
     2341 tcaaaaggcc cataaataaa tcttcatgct cttgatcccc aatgatgtct aactgatttt
     2401 ttatggcatc cggtagtgcg ggggctactg tctggctgta cgtaaaacgt agttgttcat
     2461 caattgatgc aaatccgcaa ttttcaataa ttcctttgac attgacagga atatctttcg
     2521 ctaatacaga catcatcgta gctgctccca tcgaaccacc ataaagtaaa atcttttgag
     2581 atggccgctc tcgaacttct tgatttatcc agtaaaggac gtcatctgac tcatattgtc
     2641 cataggtcac ataatcgcct ccgctattac cagtcgctct ggaatcaaca aataataaat
     2701 tatacccggc cttataaaaa tcattgtatt cttctgtaac gatcccgtta ttccaattcc
     2761 ctcgaaatcc gccatggatc atgactgttt tatctgaacc ttgatcgaca taatatgcat
     2821 atacatccat cattttgcca tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat
     2881 ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg
     2941 acatcaacac actgtttttt gcaaaagcga tatcgtacaa tagataagta gcaatcagat
     3001 gtttgataat ctctttatct acaccataat cattaacagc aagctcagtg actaataact
     3061 ctattaattt tgacttgatg tcttctgttg gtccattaag tatttctgtc atctgtttac
     3121 cgaagtaatt caaatattct ttaccattga aattttcacc tttaccgata tcatcatttt
     3181 ctgtcccatt ctctttgtca cttactttag cagaagagga ttcgtcgttt gacatttttg
     3241 tagctgttgc ttcttctgtt tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc
     3301 tagtaacaga tgtctcaacc gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt
     3361 tcgtttcatt tccagcatca gtagtttctg tggtttctac ctcccgagat tccgattcgg
     3421 aagaaatgct ctctccatat actgtccatt gcgggacgct tgtacttaca gcaaataaga
     3481 tgaccaacgc tccctttaat acttgttttg ttctcaagct attcactcct ttttatgttt
     3541 ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa aaaataaatg gtttatccca
     3601 taaaaataga acaggattat tttctcattt tacgtaataa agcagaatgg ataaaccagt
     3661 atccattctg ctttttttaa acgaagaaac aacttattgc tccaaaaatg tcagaaatcg
     3721 tttatcccaa attttctcgt aaaaagcgat tgtttccttt gctttcataa aaggattatc
     3781 ataagttgtc gttgctcctc tacacatcat aagctggtat cctaatccat gagcaaactt
     3841 cacagtcgta tctatacaat actctgcttg tgccccacat aattcgatcg aatgacagtc
     3901 caattcagtt aataaaacac tcaaattagt cttataaaat gaattcgcat gggtttttct
     3961 cacaaaataa ctgtgctcag gaatagataa ttccgacaat agctcccatg gttttgtttg
     4021 tggcactaat tgagcatctt cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta
     4081 tgttttgatt cttttattca ctaatgctgt cactgtttct aattcgtgta tcggcgaaga
     4141 atcaaaacat accccttttt gtaaatcgat cactactatt gcgtcggctg tgtttgacat
     4201 acttgtgcct tctttctttt ttcttgattt aatagtattt cttatctagc tagttccatt
     4261 atacgtgaca acattttctt attttacacc tctaaatggc acttttcgac tatttcttca
     4321 tgttattatt tcataaatac ttagctattg gaggaatgta tgatgattaa attgtataca
     4381 ggttctggct gctcgtcttc aagacgcgca aaacgttggc ttatcgtcca tgatataccg
     4441 tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa atgaatttat ccacttacta
     4501 aaattgacaa acaacggcgt gaccgaaatg ctctctactc attctattag ttataaaaat
     4561 cttgttcaca gaatagatga actcagtttg agtgaactat atattgagat tcgtacatcc
     4621 ccttctttat tgaaaaaacc gattatctac gaccaatctg caaaactttt gactggattt
     4681 agcgaagata aaatcaggat gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa
     4741 ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata atctttttgc aatgtcttaa
     4801 ttttggcata aacaatctgc ttttcttaat taacgaaatc caatttttcc aatatggatc
     4861 tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa tcattttttg
     4921 taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc tttttgtcga
     4981 taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta tattttattg
     5041 ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt ttaccatact
     5101 ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc ctgactaaca
     5161 tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc tctggaattc
     5221 tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga aatatatgct
     5281 gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt gtcccaaaaa
     5341 cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt tctgaagttg
     5401 taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca atccagcccc
     5461 aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag gtgttcatta
     5521 caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc atcgaccaga
     5581 agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg
     5641 gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct tgtttcattc
     5701 gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta cgcgtatatg
     5761 gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt gataatatat
     5821 cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat cggatacggt
     5881 attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga tagtttttca
     5941 aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata taattttttg
     6001 actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta aaatataacc
     6061 aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag aagaaaactc
     6121 gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat acctttcgca
     6181 aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt ttagatctaa
     6241 agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt tcaaaaattg
     6301 atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata ctccttttta
     6361 tctattattt agacttattc cgacagctac atgaataacg tacaaataga ttcgttactg
     6421 atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc
     6481 catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa atagatctgg
     6541 caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca ctcaaaaatc
     6601 tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc gatggaagag
     6661 agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc atgtaattag
     6721 cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat ctgtttcttt
     6781 cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta gcacactttt
     6841 taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta ccaaatcaag
     6901 ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc tactctcact
     6961 actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     7021 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgttttc
     7081 tgtgctaatt tcactgctcg tactctctgt actgctttca ccgctcgtgt tttctgtact
     7141 gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc aactgcttgg
     7201 tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat caactttatc
     7261 tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat actcaatacc
     7321 agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg aacttccatt
     7381 accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc ttttgtttgg
     7441 tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat cagttttcac
     7501 agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc caatcattgc
     7561 tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct tcatttctat
     7621 cactctccat ataattacct tctatttgat aaaggaatta tataaaatga aaatgatgag
     7681 aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt
     7741 tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca gccttgcttt
     7801 cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt tatatgcata
     7861 gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag atgcttcagc
     7921 cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc
     7981 ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc aattccaagt
     8041 aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg cgctgaaaaa
     8101 gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg
     8161 caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa atctaagcat
     8221 ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg caataaataa
     8281 aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc
     8341 cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa cgagacctcc
     8401 cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta ttctttttcc
     8461 ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc
     8521 ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg cattattgac
     8581 aagaattcct aaaacaaaac caacaaagac tattaaaaca gatccaatca accgaataat
     8641 cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct ttcttgtctc
     8701 gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga actaagaata
     8761 tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt agactgtttt
     8821 taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt gcctttcagc
     8881 atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg caacgtttaa
     8941 aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat caaataatca
     9001 aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg gaaaagagat
     9061 ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg gtccaaacta
     9121 caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac tctttaaaca
     9181 gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata aagatctaga
     9241 cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat tttcgactaa
     9301 agaagaagca gataaagcaa aaaaagaagc aaatgaagga gaaaatttct ccaagcttgt
     9361 tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga attttgattc
     9421 aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga
     9481 agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct attatctagt
     9541 aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat ctgagctaga
     9601 aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa caatccgtaa
     9661 a
//
LOCUS       DACDOA010000096         9269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDOA010000096 DACDOA010000000
VERSION     DACDOA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.096"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            254..745
                     /gene="nrdR"
                     /locus_tag="IWI39_002251"
     CDS             254..745
                     /gene="nrdR"
                     /locus_tag="IWI39_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9209311.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            768..2141
                     /locus_tag="IWI39_002252"
     CDS             768..2141
                     /locus_tag="IWI39_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9209312.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2141..3067
                     /gene="dnaI"
                     /locus_tag="IWI39_002253"
     CDS             2141..3067
                     /gene="dnaI"
                     /locus_tag="IWI39_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9209313.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3196..3636
                     /locus_tag="IWI39_002254"
     CDS             3196..3636
                     /locus_tag="IWI39_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9209314.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3780..4481
                     /locus_tag="IWI39_002255"
     CDS             3780..4481
                     /locus_tag="IWI39_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9209315.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4478..5599
                     /locus_tag="IWI39_002256"
     CDS             4478..5599
                     /locus_tag="IWI39_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9209316.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5614..6846
                     /gene="pepT"
                     /locus_tag="IWI39_002257"
     CDS             5614..6846
                     /gene="pepT"
                     /locus_tag="IWI39_002257"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9209317.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6978..7886
                     /locus_tag="IWI39_002258"
     CDS             6978..7886
                     /locus_tag="IWI39_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9209318.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7921..8193
                     /locus_tag="IWI39_002259"
     CDS             7921..8193
                     /locus_tag="IWI39_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9209319.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8204..9250
                     /locus_tag="IWI39_002260"
     CDS             8204..9250
                     /locus_tag="IWI39_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9209320.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         3120 a         1596 c         2025 g         2528 t
ORIGIN      
        1 gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag taatcaaggt
       61 gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg catgaaagta
      121 gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg tgatttgttt
      181 cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa tgcgatgcaa
      241 gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag taatcgacag
      301 ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga attgttcgtt
      361 ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca agaaaaacgg
      421 ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt cagcagaaaa
      481 aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa accgcgtacg
      541 cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga tggaagattt
      601 agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc agttcaaaga
      661 tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag aaaataataa
      721 agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg gaaaatgctt
      781 ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg ctgagagaag
      841 acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa gccttgagta
      901 tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct gaagggatgc
      961 atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat gcaagaaaaa
     1021 agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat ctgggacctc
     1081 gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa gactctgttc
     1141 tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt acccaattct
     1201 ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa tttttagatg
     1261 tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa tcgctgaata
     1321 aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca ttcgattggc
     1381 agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat ccaatcatca
     1441 aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt ggcgaactga
     1501 tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg caaaaagcca
     1561 ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg gtttctgaaa
     1621 acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag ctgataaaag
     1681 aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa aaaggcggat
     1741 atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca ggtctttcca
     1801 gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat gctagcttaa
     1861 atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc gccacagcag
     1921 aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca aaacaaaaga
     1981 agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt cctgattggg
     2041 tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag attgaccgcc
     2101 gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg taggcaaaga
     2161 attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa tgatcgctga
     2221 agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag aattgacaca
     2281 agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa agcgtaaata
     2341 tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga cgttgaattt
     2401 tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc aaagacaaga
     2461 agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat cggcttcttt
     2521 tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc tagattttgt
     2581 agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag taggaagttt
     2641 tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga ttgctggatt
     2701 caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg caatcggaaa
     2761 agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga tgatcgatga
     2821 cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg ttatccttca
     2881 atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa tggatgaatt
     2941 ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag cgaaacggat
     3001 aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca accgacgcaa
     3061 tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc aggtcagcga
     3121 atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg cagaaactta
     3181 aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg tctatttttt
     3241 cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc gattgatgcg
     3301 ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt tgtcggaacg
     3361 attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa actcgtcgat
     3421 gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga cggagtgatc
     3481 ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat cggtgacaaa
     3541 gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc gcaagttcct
     3601 ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt tcaaaaataa
     3661 gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg gaattttttt
     3721 attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg agtgaagcta
     3781 tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc cctgaatccg
     3841 ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg atattaagag
     3901 gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa tcagcacgta
     3961 agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca aatggtctag
     4021 atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg ggaggttcac
     4081 tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga caagaacgtt
     4141 tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa gaaaatcagt
     4201 ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa atcattgtag
     4261 ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc gggccatttc
     4321 tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt caacaaagaa
     4381 aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa aaacaaatcg
     4441 agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg gacgcacttt
     4501 catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag acccagtcgg
     4561 actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat tagacgttcg
     4621 tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg ctaaacatcc
     4681 tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa aaatgtatgc
     4741 agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg atattatttg
     4801 ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga gttatttagt
     4861 aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg atcatgcaca
     4921 gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt ataccggtac
     4981 aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac aaccagcgat
     5041 tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta ttttgccgga
     5101 aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg aggaacctgc
     5161 ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac gagtaggtga
     5221 attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag cgttccaact
     5281 agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc gcattgcgat
     5341 ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag cagatgtcta
     5401 tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg ggttgattgc
     5461 aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg agaaatttga
     5521 atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa caaatacaaa
     5581 cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt gccacgtttt
     5641 ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac tccatcgact
     5701 cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt aggcttagct
     5761 gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag caatgttgat
     5821 catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt taatgcagta
     5881 ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt actagatgcg
     5941 gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta tgaaggccac
     6001 acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc agggattgcg
     6061 gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca cggtaccatc
     6121 aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt cgatgttgag
     6181 caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga gttgcagtat
     6241 gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt acatccaggg
     6301 acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca taatcagctt
     6361 ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca tttgatggca
     6421 ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca tgatcgtgaa
     6481 aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa tcagcgattt
     6541 gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa agaaatcatt
     6601 gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct tggtatctct
     6661 ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta catgggtatt
     6721 ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga atttgtttct
     6781 ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt gaacacaaag
     6841 agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat ggaatgtcat
     6901 ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt ttttgaagag
     6961 taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt tcagggaaag
     7021 atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag cagccagaat
     7081 tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta gtcaagaaag
     7141 cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg attcatcata
     7201 tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt ccaggtggaa
     7261 ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa gctgctgacg
     7321 tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat gaagtcatta
     7381 tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt caagaagaaa
     7441 aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg ctgaagaaga
     7501 aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa tcccaactgt
     7561 tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac tcagcgaccc
     7621 tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc cgatttattt
     7681 tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt acttatttca
     7741 cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga acggatggag
     7801 acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta acagtttatt
     7861 caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg aagaagagta
     7921 atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa tttttactta
     7981 ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg tgttcctgag
     8041 aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat ctctcgaaaa
     8101 gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc aggtgtactt
     8161 gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt ctaaaggaac
     8221 gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca ttatttatta
     8281 tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca gtgttgttgt
     8341 tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc gctcaatcaa
     8401 agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct tgacctctag
     8461 ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct cggagattta
     8521 tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag cactccatat
     8581 gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca tccaattctc
     8641 tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta tccttttgac
     8701 gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc aaatattttt
     8761 tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg tggatacatg
     8821 caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag acaggactgc
     8881 gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg tacttcctta
     8941 tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt cgtttatcag
     9001 ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt cctttgaatt
     9061 tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt cttcgctttt
     9121 actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt atgtgattgc
     9181 tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga ataaagaaaa
     9241 tcaagggtag gtcttttata agatctacc
//
LOCUS       DACDOA010000097         9207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDOA010000097 DACDOA010000000
VERSION     DACDOA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.097"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..606)
                     /locus_tag="IWI39_002261"
     CDS             complement(<1..606)
                     /locus_tag="IWI39_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9209321.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQL"
     gene            740..1027
                     /locus_tag="IWI39_002262"
     CDS             740..1027
                     /locus_tag="IWI39_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209322.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1443..2981
                     /locus_tag="IWI39_002263"
     CDS             1443..2981
                     /locus_tag="IWI39_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9209323.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2978..3943
                     /locus_tag="IWI39_002264"
     CDS             2978..3943
                     /locus_tag="IWI39_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209324.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3930..5252
                     /locus_tag="IWI39_002265"
     CDS             3930..5252
                     /locus_tag="IWI39_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9209325.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5315..5419
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5493..6236
                     /locus_tag="IWI39_002266"
     CDS             5493..6236
                     /locus_tag="IWI39_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209326.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6251..7243
                     /locus_tag="IWI39_002267"
     CDS             6251..7243
                     /locus_tag="IWI39_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9209327.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7255..7992
                     /locus_tag="IWI39_002268"
     CDS             7255..7992
                     /locus_tag="IWI39_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209328.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7982..8704
                     /locus_tag="IWI39_002269"
     CDS             7982..8704
                     /locus_tag="IWI39_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9209329.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8788..>9207)
                     /locus_tag="IWI39_002270"
     CDS             complement(8788..>9207)
                     /locus_tag="IWI39_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9209330.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3016 a         1592 c         1947 g         2652 t
ORIGIN      
        1 caattggggc aaaacagatc caatttccag atacttcact ttattcaatg tataagatag
       61 aatagaacag ctaagtaatg tatacagaaa atgtgtgaga tccaaataaa gcaaggagac
      121 aactaaaata ccaaaagtaa tcaagaaaag acttttgcca atcttaatat gaaaatattt
      181 ttcaatggct tgataaatga tatctgttcc gcctgtgctt ccgccgaatc gaaagacgag
      241 accgcaacca atgccagaaa gaatacctgt catcaaacta ttgatcagta gactatctac
      301 agcaaaatgt atatgaaggc tctcccataa atgtaaaaag aaaactagcg caaatgttcc
      361 gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac aatgagataa gcaataaagg
      421 taaattaaaa agcagtgaag tgacagaagg agcgaatcca aaaagattat gcaaaagcaa
      481 agcaatacca gtaaccccac catctgccag cttagatgga atattgattc ctgtcacagc
      541 taatccgtag attcctgcgc ctaaaatgat tccgcacaat tctgtaaata aatttaattt
      601 tttcatttct agcctctttt cttttgaaaa ttttcagcaa cttctttatt atactgactt
      661 atactcttac ttcaagagga aaatgaaaaa aaattatttt tggctgtttc tttttattta
      721 tcaaataagg agtggaaaaa tgcgagcagc agacttattg acaattctta caaaatcacc
      781 caaaaaaaca gagtatcaag gagttttttt ggctcaagaa caaaaactgg tgcctgtgac
      841 aatggtccag ataaataaag caaatcaatt tatcttttta tttgaacacg gcaaagcacc
      901 acttgctatg aaagacatct ttcttttttt gatgaagaac cgtgagaaag ctgtttatta
      961 tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa gagatagaaa ataaagtggt
     1021 gatataagta aaaataagaa aacaagacgt ttatgatgtc aaaacaacca ttttttacat
     1081 ttttggcaaa aaagtgtcat gatacagtat aatattcgtt gaacaggcca ataaaggtgt
     1141 tctcccccac gagatttcta ctttattggc tgtttctttt tattaaaata aaagttgtgt
     1201 gtgtaaaaaa gatatagctg attccaagtg acaatttcgt atagattttt atggtattcg
     1261 tagtgaaaaa acgagaacca aaaagttgat ccgactttgt tgcttggatt ctttttttgt
     1321 cagcatattt agaggtattt ttttcttttt tttgttcgtg ctgtaaggtt aaggcaagac
     1381 aagttagttg ggaaaaagga aagaaatgtt tatacttaaa gaataacaga aaggaaggtc
     1441 gcatgaataa ttttatacaa acattaagtg aaagaaaagg cgagttgctg gaagctgcat
     1501 ttcaacatct gtccatttct ctcatttctt tgctgattgc agcactgatt gctattcctt
     1561 tagcgatttg ggcagccaat cataaaaaaa tggcagaagt cttattgcag atcacgggtg
     1621 tgctccaaac gattccttca cttgccttat taggtttact tatcccattt gtagggatcg
     1681 gaactgttcc agcattgatt gcacttgtta tttacgcttt gcttccgatt ttccaaaaca
     1741 cgtatattgg tctagctgaa attgacccgt ctattgaaga agcagcggta gcatttggta
     1801 tgtccagaat gagacgcttg ctgaaagtag agcttcccat cgcattacct gtcattatct
     1861 caggtatccg aactgcctta gttttaatca tcggtacggc tactttggca gcattgatcg
     1921 gagcaggtgg attagggaca tttattttgt taggtattga ccgaaataca cctgttttga
     1981 cattgattgg tgcgatcagt tctgccttgc ttgcaattat attcagcgga ctgattcgct
     2041 ggttgcagca taagaaacct cgatattcca tcattacttt agcagcagtg gtgtgtttat
     2101 ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa tgaaaccatc acgattgctg
     2161 gaaaattagg agcggaacct gaaatcctga tccaaatgta taaagaattg attgaagaca
     2221 acacaaaaac agaagttgaa ttaaaaccga attttggcaa gacgagtttt ttgttcaatg
     2281 cactggatca aggtcaaatc gatatctatc ctgagttcac aggtactgtc ctagaaagct
     2341 tggtgaaggt acctgaagca atgaaaagtg ctgatttgac aaaagaaaaa acatatgacg
     2401 aagcagaaga attattgaat aagcaattcg atatgacatt attaaaacca atggcttacc
     2461 aaaatacgta tgccttggca gtcaagagat cttttgcgaa cgaacatcaa cttaaaaaga
     2521 tttccgattt ggcagctatc tctgatcaga tcaaagcggg attcacactg gaatttattg
     2581 atcgttcaga tggatataaa ggaatccaag aagcatatgg cttgaatttt tcaagtgtac
     2641 aaagcatgga gccaagtttg cgttaccaag caatcaataa tggagatgtc aatattgtgg
     2701 atgcctattc cacagatagc cagctcaagg agtacgatct agtgactcta gaagatgatc
     2761 gagggttatt cccagcttat caaggcgcac cgctgatgaa aaaagatttt gctaaagaac
     2821 atcctgaaat cgttaaagca ctgaataaat taagcggaaa aataacagag gatcaaatga
     2881 ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc gacagaagta gcacgaatct
     2941 tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg actaaattaa tcgagttcaa
     3001 acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat gacctaaatc tggcaatcaa
     3061 aaaaggagaa atctttgttt tggttggtcc ttccggtagt ggaaaaacaa ctactttgaa
     3121 aatgatcaat ggattatcta aaccatcagc aggagacatc tacttcaaag gaaaatcgtt
     3181 aaatgaatat aatctgcaga aaatgcggtg gaatatggga tatgtgctgc agcaaatcgc
     3241 tttgttcccg accatgacag tcaagcaaaa tattgaagtc atccctgaaa tgttaggttg
     3301 ggaaaagcag aaaagagcag accgtgtaga tgaacttctt caaaaagttg gcttatctcc
     3361 tgatatttat cgagatcgta tgcctagaga gttgtctgga ggagaacagc aacggatcgg
     3421 gatcatccga gctattgctg caagcccaga tgtgattttg atggacgagc catttagcgc
     3481 attagatccg atttcaagaa actcattaca agaattggtt ttgtccttac acgaagaatt
     3541 gggcacgacc atcgttttcg ttacgcacaa tatggaagaa gcaatcaagc taggtgatcg
     3601 tattgcattt atgaaggatg gagaaattat ccaatgtgac acaccagagc agttattgat
     3661 gaatcctaaa aacgactacg ttcggcattt ctttgatgaa ccgaaacaga cgaaagagtg
     3721 gcgagtagaa gatttagtcg tcaatggata tttcttaaat gaaataccag aaaatgctcg
     3781 tcaacaagtt tgctttgata cgcctatgaa agaagtatat tcgcttcttt cggtgtatcc
     3841 aagcattgtg atagtagaaa agcaaagaag tattgggtcg ctgacaagta aagaaatctt
     3901 tgctttttta agtcgagagg aggaaggaaa tgaaaacata tgatgcaatc atcatcggtt
     3961 caggtgtgag cggcttaagt gctgcttatg gattgaaaga agcaggtaaa acagtactag
     4021 tggtcgaaga agatctatgg ggcgggactt gtcctaaccg tggatgtgat cctaaaaaag
     4081 tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca attgagtgga aaaggtttta
     4141 acgagatccc aactgccaac tgggaagaac ttcaaaaatt caaacgaaca tttacggatc
     4201 cagtcccaga aagccgaaaa aaacaattag cagaagctga aatcgatcat ctttcaggaa
     4261 ccgctcgttt tttggatgat tcatctattg aagtcaacga agaagtcttc catgcggatt
     4321 acttagtttt ggcaaccggg caaagaccta cgatcttacc agtggaggga aaggaatact
     4381 tgaaaactag tgcagacttt ttatcattgc ctgtactgcc taaagaaatc atttttatcg
     4441 gtggtggtta tattgctttt gagctagcta cgattgccaa tgctgcaggg agcaaagtaa
     4501 ccattgtcca tcataatcaa cggccattaa aagaatttga ggcgtctttg gtagaggaag
     4561 ccgtccatca aatggaagca tctggcattc agtttgcttt tggtgttgaa acccaaaaga
     4621 tcatttcaga aggtactcgc tatcgtttag tcggaaaaga gacggaatta gtagcagata
     4681 tgattttttg tgcgactggg agacagccga atacagaatc tttagcactt gagcaagcga
     4741 atatcgtctt tgataaacac ggaattgcag tcaatgatta tctgcaaacg agcaatccaa
     4801 aaatttttgc gtgtggagat attgtttcaa ggaaaacccc aaaattgaca ccagtcgcca
     4861 cttttgaagg aaattatgta gctaagcgga taacggatgc aacaagcgaa cctatcaaat
     4921 atccgattat acctacgatc gtctacgcca gtcctaaact tgctgaagta ggagttacaa
     4981 aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga tcttaccagt tggttcacct
     5041 atcatcgtgt caatgaacca gttgctaagg ctgaattgac ctttgaccag caaaattatt
     5101 tgattggtgc tgcagtgatt agcgagcaag cagacgaatt gatcgatgat ttgacactcg
     5161 tcatcaatca aaaattgact aaaaaagagt tggatagcta tatcatgggg tatccgacat
     5221 tggcaagtga tctctcctat ttattgaaat aatcagcatg ataactaaag cgcttgttgc
     5281 tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg tttctgggga tgtcgtagac
     5341 gacatgagag taagttgtga acttagaccc attgaacctg tcagttaata ctggtgaagg
     5401 gagaaacata gcagacgctt gaagcgggtt actatgtcgt gcccatttca agcgtttttt
     5461 gttattcata ttagaagaaa ggaggtaaca aaatgaagaa aaatctttat ccagcagtca
     5521 gtatggcatt cattttaatt atttggcaga ttgttgtcat agtttggaaa atacctgcct
     5581 ttattttacc cggacctgct gctgtatttt ctgcattagc ggcagatgca caagtattac
     5641 tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg tttggtaata gctagcgttc
     5701 tttcttttat tactgccttg gcaatggatt attggaagtt tttagaaatg tctttttatc
     5761 cgttactagt gatttcacaa atgttgccaa tcatggtgtt agggccatta cttaccctat
     5821 ggtttggttt cggtatgctt ccaaaagtga tccttgttgt cttgataagc tattttccga
     5881 tcgtagtttc tttttcggat acattgagga aagtatcaaa agagcaaatc atctttttga
     5941 aaacgatggg ggcagataca gggaagattt atcgaatata taaaatacca gcgggtttat
     6001 ccggcttctt ttccggttta aaagtggctg ctacttattg tgtcagcgga gcagtcgttg
     6061 gagaatggct aagcgctcaa gcaggcttag ggtattacat gatacgcgta aaaaacagct
     6121 accagatcga taaagttttt gctgcgatca tttgtgtcat tctattgagt ttattgctga
     6181 atggcgcttg ttcattattg aaaaaagggt attatcgtat gctttatatt ttttaagaga
     6241 aggggatcaa atgaaaaaat tatggctttt attaccgcta ctgttgcttt tatccgcttg
     6301 tggcactgca aaagaaacaa gtagcaaaca agaaataaag gatttgaaaa aagtgacact
     6361 tgttttggat tatgtaccga atactaatca tactggcatc tacctagcaa aagaaaaagg
     6421 ctattataaa gaggctggtt taaatgtcca aatcatcgaa ccaggagata attcgacaag
     6481 tattggtcta gtaggtgcgg acaaagcaca atttggtgta agttatcaag aagatgtcac
     6541 ttatgctcac gcagatggac agaatatacc agtcaaagca attgctacag tgatcaaaca
     6601 taatacttct ggattcgcca ccctatcaga cagtaacatc cattcaccaa aagattttga
     6661 aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa gctgtcttaa aagctgtgat
     6721 ggaaaaagac ggcggggact tttcaaaact gacaatggta ggaagcaatg gagaagggcc
     6781 agaaagctta ggaaaatcct cagatatcca atggtacttt gaaggttggg atatgatcaa
     6841 agcaaaagaa gcgggtattg aactaaacta tatcccattg aaagaattag atgaacggtt
     6901 agattactat actccggtaa ttattacgaa tgatcaactg atcaaaagtg atccagaatt
     6961 ggtacagtct tttatggatg ccacgaaaaa aggctatcag gaagctatta aggatccaaa
     7021 tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat gatcggacgt ttttagaaga
     7081 atcacaagca ttcttgtcaa aaaattatac ggatgatcct gagaattggg gattgatgga
     7141 agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa aacggattga tcaagcaaga
     7201 tgtaccttcg caagaactat tcactaatca gtttataaaa taggagtgat agatatgtca
     7261 atccaaatca gccacttgaa tgtagtcatt gaaaacctga aaatattaga agacatccat
     7321 ttcactatcc ctaaagatac ctttacatcg attgtcgcgc caagcggagc cggtaagtca
     7381 acattgctga aaactttaac aggagtccag cctatgactt ctggaacgat caaagtagac
     7441 gatcaaaaag taactggtct ccatacagca tttagttata tgccgcaaga agatatgtta
     7501 cttccttggc tgagcgtcta tcaaaacgtt acgttatatc aaaaaatcaa tcatctaaca
     7561 atcaatgaag aacaagtaag ggattatctg gatatctttg ggttaaacgg ctatgaacat
     7621 tttttgccgg aacaattatc tggcgggatg aagcagcgaa cggccttgtt acgaacaatc
     7681 atgaatcctt cttcttactt gcttcttgat gaaccatttg gagcgttaga tgctatgacc
     7741 agagggcaga tgcaagactg gatgttgaag ctgcctaaaa aagcaaagcg gactacttta
     7801 cttgtgacgc atgatatcga agaagcaatt tatttatcag acaggattct tgtgctttcc
     7861 gcccgtccgg cacatgtgat tgctgagatt caagtgcctg aaaaacgacg gtcacgtgaa
     7921 tggctactgc agcaatcaga gttgaagcaa acgatttatc agttattggc aggtgaatta
     7981 gatgttaagt gatgctcatt gtcatttaga tgggagtcag agtttagcac tccttcagca
     8041 ggaacacacg attttgacga tcatcaattg tgattcgcca gaagaatgga aggaaaacag
     8101 acagcttgca gcaagtaaga ctcaagcctt aagttacggt atccatcctt ggaaagcaga
     8161 tagctatacg tttgaacaag ttgagccttt tttgaaaaaa gcaaggatca tcggtgaaat
     8221 agggttagat aatatttgga cgaatgtacc gatgactaca caaaaaaaag tatttgagcg
     8281 acagcttgct tttgcagcca taaatgagaa acctgttgta ctgcacacaa aaggatgcga
     8341 aaaagagatc ttaaattata tccaagctta ccctaatcac tatttgatcc attggtattc
     8401 ttctttggac tatcaacaag actatattga tttaggctgt tatttcagca ttggtcttga
     8461 tttgaaaaag aatccagcag tctggcaact agctaaagca gtcccaatcg accacttact
     8521 tattgaaacg gatggactac atgcagcaga gtgggcttta ggggggattt tttcagaaaa
     8581 agattatgtc tcactgcttg aagaacagtt aaagacaatc gctgagatca aagagctatc
     8641 agttgaagaa gttaaaaaaa atacttatca aaacttggaa cgtttcatta gattaggtaa
     8701 ataaaaaaga gatgtgacac aactagtgtc gcacctcttt ttggctttct ttgtataaaa
     8761 gtaaactgtt tgattaataa atgaatctta gtaccaaccg tttgcttccc agaatgcttt
     8821 cgccgcttcc caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt
     8881 tgcagcagag tagtcaccat ttaagtaaga agaatctaat tggtaacgtc cgatgtaacg
     8941 tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga
     9001 gcttgtgctt gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc
     9061 aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg
     9121 gactggtgct tcttgaacgg ctgcttgagg ttcagcagct gcttgtgcag agccttctgt
     9181 tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACDOA010000098         9173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDOA010000098 DACDOA010000000
VERSION     DACDOA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.098"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(245..1294)
                     /gene="recA"
                     /locus_tag="IWI39_002271"
     CDS             complement(245..1294)
                     /gene="recA"
                     /locus_tag="IWI39_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9209331.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1403..2644)
                     /locus_tag="IWI39_002272"
     CDS             complement(1403..2644)
                     /locus_tag="IWI39_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9209332.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2884..3462)
                     /gene="pgsA"
                     /locus_tag="IWI39_002273"
     CDS             complement(2884..3462)
                     /gene="pgsA"
                     /locus_tag="IWI39_002273"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9209333.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3671..4555)
                     /locus_tag="IWI39_002274"
     CDS             complement(3671..4555)
                     /locus_tag="IWI39_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209334.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(5332..6618)
                     /locus_tag="IWI39_002275"
     CDS             complement(5332..6618)
                     /locus_tag="IWI39_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9209335.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6611..7879)
                     /locus_tag="IWI39_002276"
     CDS             complement(6611..7879)
                     /locus_tag="IWI39_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9209336.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8131..8592
                     /gene="mscL"
                     /locus_tag="IWI39_002277"
     CDS             8131..8592
                     /gene="mscL"
                     /locus_tag="IWI39_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9209337.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8651..9097)
                     /locus_tag="IWI39_002278"
     CDS             complement(8651..9097)
                     /locus_tag="IWI39_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209338.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2537 a         1975 c         1623 g         3038 t
ORIGIN      
        1 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
       61 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
      121 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
      181 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
      241 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
      301 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
      361 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
      421 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
      481 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
      541 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
      601 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
      661 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
      721 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
      781 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
      841 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
      901 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
      961 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
     1021 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
     1081 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
     1141 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
     1201 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
     1261 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
     1321 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
     1381 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
     1441 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
     1501 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
     1561 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
     1621 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
     1681 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
     1741 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
     1801 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
     1861 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
     1921 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
     1981 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
     2041 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
     2101 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
     2161 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
     2221 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
     2281 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
     2341 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
     2401 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
     2461 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
     2521 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
     2581 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
     2641 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
     2701 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
     2761 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
     2821 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
     2881 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
     2941 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
     3001 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
     3061 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
     3121 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
     3181 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
     3241 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
     3301 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
     3361 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
     3421 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
     3481 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
     3541 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
     3601 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
     3661 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
     3721 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
     3781 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
     3841 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
     3901 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
     3961 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
     4021 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
     4081 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
     4141 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
     4201 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
     4261 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
     4321 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
     4381 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
     4441 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
     4501 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
     4561 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
     4621 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
     4681 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
     4741 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
     4801 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
     4861 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
     4921 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
     4981 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
     5041 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
     5101 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
     5161 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
     5221 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
     5281 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
     5341 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
     5401 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
     5461 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
     5521 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
     5581 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
     5641 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
     5701 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
     5761 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
     5821 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
     5881 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
     5941 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
     6001 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
     6061 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
     6121 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
     6181 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
     6241 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
     6301 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
     6361 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
     6421 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
     6481 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
     6541 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
     6601 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
     6661 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
     6721 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
     6781 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
     6841 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
     6901 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
     6961 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
     7021 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
     7081 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
     7141 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
     7201 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
     7261 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
     7321 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaat cctgggatct
     7381 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
     7441 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
     7501 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
     7561 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
     7621 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
     7681 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
     7741 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
     7801 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
     7861 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
     7921 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
     7981 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
     8041 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
     8101 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
     8161 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
     8221 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
     8281 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
     8341 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
     8401 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
     8461 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
     8521 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
     8581 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
     8641 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
     8701 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
     8761 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
     8821 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
     8881 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
     8941 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
     9001 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
     9061 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
     9121 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tct
//
LOCUS       DACDOA010000099         9066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDOA010000099 DACDOA010000000
VERSION     DACDOA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.099"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IWI39_002279"
     CDS             4..2376
                     /locus_tag="IWI39_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209339.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IWI39_002280"
     CDS             2437..3897
                     /locus_tag="IWI39_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9209340.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="IWI39_002281"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="IWI39_002281"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9209341.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IWI39_002282"
     CDS             6353..6946
                     /locus_tag="IWI39_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9209342.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IWI39_002283"
     CDS             6959..7858
                     /locus_tag="IWI39_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9209343.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8346
                     /gene="ssb"
                     /locus_tag="IWI39_002284"
     CDS             7861..8346
                     /gene="ssb"
                     /locus_tag="IWI39_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9209344.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(8487..8690)
                     /locus_tag="IWI39_002285"
     CDS             complement(8487..8690)
                     /locus_tag="IWI39_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9209345.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            8765..9025
                     /locus_tag="IWI39_002286"
     CDS             8765..9025
                     /locus_tag="IWI39_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209346.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
BASE COUNT         3441 a         1518 c         1896 g         2211 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac taaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aagacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccggg acaaatccag tcaacagatt ttggatcaac
     5761 gttacaagag gtgaaagcag ccagtgaaaa acaggaagca acgcgtcatt taggtgtttg
     5821 tccaaaatgt cgggaacaag aagtcttgct ttatcaaaag gtggcggcat gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag ccacacaact
     5941 gaaagagatc attcaaaacg gccgaacaag ccaatcagtc agaggactaa aaggtcagaa
     6001 aggttcgttt gaagcaacga tcgttttaaa agaagacttt acaacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatgaagggg
     6121 ttatcctatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgtttagaa caagagaaat tacgtgcaaa taaaagaaga ctgcaacaat taaagaacca
     6241 gcagcgcatt caaaaagaaa tcgaattaga tcgatagacg ttaatcttgt tacaaataaa
     6301 ctactatgat atatttgatg tagtgtattt gtaacgaagg gaggggaagt aaatgcaaga
     6361 taaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccaggaa
     6421 atttggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgattttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatggc tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaatt tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagtg caacggcatt ttatgatgga tcgtctaatt gaacaaattt ctgaatcaaa
     7081 gtacaaaaat gacttcattt tgaaaggcgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggaca acaattgata tcgatacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagc atttttaatg atttaactag aaaacccaca aaagaaggca ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtacc gtttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaagtgga
     7441 gattccagct tatccaacag aacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcagag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaacg
     8041 atggcgacat tagctaaaaa gggcagtttg atcggcgttg ttggacggat tcaaacccga
     8101 acctacgata accaacaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagtaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccagc
     8221 agtccaagcc ctaccacctt tgaacaacgg gatgtagcta cgcctaacaa caatggattg
     8281 aacgcttcac aaaatccatt tggcggacaa tcaatcgata ttagtgatga tgatttacca
     8341 ttctgattgt aaaagatcaa taaagttgtt ataatggata cgaaaagagg agttgcgcta
     8401 acaactcccc taacagcacc gtttaagacg gtgaccagct taatattatt tttgaaaaat
     8461 aatcgtcgac cggctaaagc tgacggttat tttttttgat tgttgtttac aactgctaag
     8521 attccaaaaa tgattgttgc gatcaaagaa ccgaaactaa tcattaatcc tagcgccgtt
     8581 tcaacaggca aaaggcttct cctttctgta agattttgga agtacgcaca tacgcaccac
     8641 ctcactttca atcggatagc caccgtcaca aactttactg tcttgatcat tatagcatat
     8701 ctaaaatcga ataaaaattc tcaattttag ctgtttttgc tataatttta gcgaggtgat
     8761 cgtgatgaac aaatatgttg gtttgcttga taaaatccga gtaataaaaa cacaaccatt
     8821 attggttcgc tttactttac aaacaattca tgaatcgatt aattgtgtag ttgcagatat
     8881 tgaaattatc gataaattgt taattatgga tgatggaaaa tacaatattg ccgtcacagg
     8941 tcatttcaat aaacggaatc aattagtcat tgaatcgatg caaattagaa accccgatca
     9001 ttttacgcga tcgatgggca tataaaagaa gagctgcagt cctaaatgag gattgcagct
     9061 cttttt
//
LOCUS       DACDOA010000100         9017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDOA010000100 DACDOA010000000
VERSION     DACDOA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.100"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..877)
                     /locus_tag="IWI39_002287"
     CDS             complement(53..877)
                     /locus_tag="IWI39_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9209347.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(850..1530)
                     /locus_tag="IWI39_002288"
     CDS             complement(850..1530)
                     /locus_tag="IWI39_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9209348.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1553..2491)
                     /locus_tag="IWI39_002289"
     CDS             complement(1553..2491)
                     /locus_tag="IWI39_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9209349.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2736..3587)
                     /locus_tag="IWI39_002290"
     CDS             complement(2736..3587)
                     /locus_tag="IWI39_002290"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9209350.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3741..6314)
                     /gene="mprF"
                     /locus_tag="IWI39_002291"
     CDS             complement(3741..6314)
                     /gene="mprF"
                     /locus_tag="IWI39_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9209351.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6455..7261
                     /gene="proC"
                     /locus_tag="IWI39_002292"
     CDS             6455..7261
                     /gene="proC"
                     /locus_tag="IWI39_002292"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9209352.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7340..8896)
                     /gene="rny"
                     /locus_tag="IWI39_002293"
     CDS             complement(7340..8896)
                     /gene="rny"
                     /locus_tag="IWI39_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9209353.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2651 a         2001 c         1557 g         2808 t
ORIGIN      
        1 aagaagccta taaaaaattt ttcgttttta taaggcttct tttctttata atttagttat
       61 tttttcttct gaaggcaacc aatattcgtt tgatgatatt tacaatcagg aacaaagcaa
      121 taaagactaa tgtgattgtt gctcctggag gtgtatctag ataaaacgag ctagttagtc
      181 ctgacagcat ccctagaaat cctacacaaa cactgatagc aatcacgaca ttgaagcttt
      241 tgcctaatcg cataccgatt gctgcgggaa gaaccatgat tgcggatatc aaaagtgcac
      301 cggcaatcgg gatcatcaca gcaatagcca caccagtaat cacattgaac atcatcgaca
      361 tcaaatgaac gggtagccca tccacgtggg cggtatcttc atcaaaagtc agaacataca
      421 tcggccgttt gaataaaaag aacaatatac cgatggcaac gaataagatt cctaaaaaga
      481 tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag ataggattgg atacttgttg
      541 ctgaattccc tccgctcaaa ttcatcaata ctaacgctag cgccaaacca ccagccatta
      601 aaatggcaat ggagatttct gaataggttc ggtagatcgt tcgcagatac tccaatatca
      661 cagctgctaa tacaacgact agcatggtcg taagtgtagg attaaggttc aaaaagaatc
      721 ctaacgctac ccctgctaaa gagacatgag aaagcgtatc agccatcagc gattgtcttc
      781 tgatcactaa aaacacacct agcatcgggg cgattccggc aatgaagata gcggcaagaa
      841 acgctcttct catgaattca tatgaaagca acgccattga gaatcctcct ttctcaccag
      901 tcggatctgt ctgtccgcat actgtttgat atcttcatga tcatgggtga tcattagaac
      961 tgatttccca tggacatgcg cactatgtcg gagaagacga taaaattcat ttctggattc
     1021 ttcatccata cctgttgttg gttcatcaag aacaaataag tcagggtcag tagcaaaaat
     1081 acgcgctaaa ctgattcgct gcttttgtcc gccagacaat tcacctattc gccgatttcg
     1141 catatcccac atccctacag cttctaaagc acgctgtaca tgttgatgat ctttttcaga
     1201 gagacgcttg aaccagcggc ctcttggata tcttcctgaa cggactaatt cgatcaccgt
     1261 actaggaaaa cctgcattga aagatgcaac ttgttgagga atataaccga tacttaattt
     1321 ttttccttct tgatttttct cagcaatctt tatcattcct tttgaaggtt tcagtaatcc
     1381 taacgatgct ttcaccaagg ttgacttggc tgctccattt tctccagtta aaatgacaaa
     1441 ttcacccgca tctacatgat aagagacatc ttctagtaca ggttcatcat catagtaaaa
     1501 ggtcaaatca gatatttcga tataacgcac cttacaacct ccattctaaa acttaattga
     1561 tactttcttt taaagctttc agattttttt ccatgacaga aatataattt tcgcctgctt
     1621 tgatttgttc attagtcaag ctttctaaag gattcaacac ttctagcttc actcctgttt
     1681 cattcgacaa tgtctcagct accttcgaag aggcattttc ttcaaaataa atcactttga
     1741 tgttgttttt cttcacaaat tcttttagct cagctaagcg acttggtgtg ggttcctgat
     1801 ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta ttgattcgct aaataagcaa
     1861 aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc ttttagctca tcggagtatt
     1921 tttgatccag tgcttcaagc tttttgatat aagcatcggc attcttcgta aaagtttcct
     1981 ttttatctgg atatttttcg cctaattctt cagcaatcgt tctgacttct tttatagcta
     2041 ataccggatc gagccaaaca tgtggatcgt attcatgtga atgaccatgc ccttcttctg
     2101 attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc gattccttta gctgcttcga
     2161 tcatcaaggt ctgtttgcta tccactgaat cctttaacga atccacaaaa aattccatgt
     2221 ctgaactatt atacacgaaa acatctgcat cggaaatttc tgctctttct tttgcacttg
     2281 gttcgaaatc atgcggctcc gttccagcag ggatcaaaag tttcacgttt ccttcatccc
     2341 ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt cgtaacgatc gtcaaatcct
     2401 cttttttgtc tgcttccttg ttcgtatttc cacatgctgc aaacagtaaa gcacctatca
     2461 ttaccgtagc tgtcaaaaga tattttttca tttatatgct cctttcaatg aacaaatcgg
     2521 aattttacga tttattaacg tgaactaatg tatcaaaata aaaaaacaca gtcaatagaa
     2581 atccatcaat ttctgattta agcatgtata ttcttgatta aatcgtaaaa tggatgagtt
     2641 gctgctcata tctagccatt tcactacaat aaaaaactga agaataccat tttaggattc
     2701 ctcagttttt catcacctat tttacttctt atattttatt gcagggttcg tactaaataa
     2761 acttcctcac aaaaaccttt taacccatta tatactctct gcgctcttga ataatgttga
     2821 cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg cagcatctgc tccgtacttg
     2881 atcatacgct ctttgatttg atggatgact ggatgccgct ttcccgttat atcttctaaa
     2941 ctgtttccca tttttttgat catctgctga taatcttgct ctcggatggc ttcagcaagt
     3001 ccgtggatgt cttgatgttg gatggcttgc gtatctactt ctctaaacac tgtacgtgta
     3061 gagacactca ttcttggttt gaccaacaca acccagcact gtggcataga aggtaatact
     3121 tggatttctt cccctttgcc accgacaaat gccgtacttc cgtagacaca gtaaggaaca
     3181 tctgtaccga cttccaaacc gatttctgcc agttcttcta acgaacagcc cagattccat
     3241 aattggttga tccctcttag agcagctgca cagtctgtac ttcctccgcc taatccagct
     3301 gctactggaa tattcttctt aacaaagatc ttgacaccgg ttttgatatt gtatttattt
     3361 tttagcaata aagcagcttg ataaacattg ttccgttcat ctaccggtaa aaatgcttta
     3421 tttgtttcta caatgatttt gtcttctgtt atttcttcaa aaaatagatg atctgccaag
     3481 tctacgctcg acatcaccat ttccaactca tgatacccat cttgccgtct atataataca
     3541 tccagtccta aatttatctt ggctggcgct ttttcaatca attccatcct ctcacccatt
     3601 cgaaaagtct aagtcgcaag tattctatca tagggaaaaa taaaagtata gcttatgtct
     3661 gcctagtggt tgattttgtc atcgtttcaa cagctttcac agaattttct cttcataatc
     3721 caaacaagtt ctagtgattc ttataattct gtgagcactt cttctgccaa actcggaaca
     3781 tttccttttt cttttcctat cagactgagt agctgcatgg tagcaaatcc gaaattactt
     3841 cctttcctat aagcaaaata acgtccttcc caatgatcga catattttaa tttcttttca
     3901 taagcagact ggaaggaata gaaaaaatta ccataattat atacgatatg catgactttt
     3961 tcttgaaaga aagaaaaatc tgtttctcca acattagcta atggagctaa gcctagattg
     4021 gcttccgagt agcccatttt ctcgtataca ataaacagat tggctaacag atagtcagct
     4081 aattcctcag attctttagg taagacccgc aacaaatcat atcccgcctg cttcttcgtc
     4141 atcggctgag cggtgatgaa gccaacgact ttttgatctt tacggacaat accgacaccg
     4201 ctagataata catatgattt atcaaatcgt cccacagaga agtatttttc tcgtgcacct
     4261 tgaagccatt tttgtgaaac ttcatttaca gcttgatgta attcttccgg cagttgatcg
     4321 taccaagtaa aatggtatcc ttctttcatc aaacgttgat attcaagttg ctgactcagc
     4381 cgagacaagt cttctgttgc tagatcaaca atccctgctt cacctacttt cgtgaagtga
     4441 aaacccaatt catgaagcat cactacgtag ctttctgata tcttataaaa cgcaagctga
     4501 tagcctaaac aatctgcttg tttcataaac tgattcgttg ctgcccgcat cattttagga
     4561 ttgccaactg gatctcctaa aacaaagcat ttgtttcctt tgatctgata accaaaaacg
     4621 acttgatctt ggtcgtcttc ttggtaataa aaataactgt aacctgacaa gcgaagctga
     4681 tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt caaaacggtc tttttcgtac
     4741 tcactgccaa gcttcgtagt atcttcagct aaatattgat aaagcgccac taaagctagc
     4801 aatgagatgc ccaaaccaat aagccctgag aaccacacat catctgaagg aaacaaaata
     4861 aaatgatttc ctgttattcg accgactaaa aagcgtccgc tgtgatagcc tgcaattgcg
     4921 taaacgataa acaaaaagcc gaacaagcta gcatcaaaga ttatagctcc ccaagaataa
     4981 acgaattttg ttcgataaaa ttcttttctg gataaccaca cagctaacag aatcaagaca
     5041 tataccaata tcaactgcca agataacgta cgtgtgattg tatttaaaat accaaaagat
     5101 agtaaagcaa tcgtaggcag aaatgccttt ttgactttta ctgaaagtgc tctagcaagg
     5161 ccgagtaata aaaagccaac caatagattc actgtctgat ctaaaaaatt gaatgagaac
     5221 ggcaataata cttgaaatag ccgactgatt gcagacagat tagtgattgt tgatagaagt
     5281 accatcatga tccccgcaaa atagacagca cttacgagca cgatatgcgc aaccttttgg
     5341 aaaaacagtc gcggaatgtt atctaaaaat cgattgattt tgactcctgt atgtgtgatg
     5401 aacaatagaa gtcccgtcat aaaaggtaga acataataaa acaaccgata atataatagc
     5461 caaactactg ctacatcttg ccgaacaggc aattgactca ttcctaagat catcaaaacg
     5521 tcaaaagtcc ccattccacc tgggaccatc gtcagcattc ctatcaatgt agcaatcatg
     5581 aacataggat agatcgaaag catcgaaata tctattctca tcaacttgcc gatgatcaaa
     5641 aataccgcaa gggctcccgt ccattgtcca aaggatgcca gtagcagacc aagcaagcct
     5701 tttggataaa actcgaagaa caatgtcttt ctctttagat aagcaaaaaa gaaaatgact
     5761 ggtgctaaaa aactaccgcc gaaaagccag atccaatagc ttcgaaaaat actttctgac
     5821 tgaataaaaa agacatcaat aaatgtaaga aacgaccata cagacaaacc agtcagcata
     5881 aatagtgcga cttttgatac agtggctaaa accatttttc tatttgtctg ctttccataa
     5941 aaactagccc gcagacttgc tcctaccaca ccaccaaaac cggcgagatt gttgatcgtg
     6001 tttgttaccc acgcagcaag aagaaactct tttctttcca tttttggttt tccttgtttt
     6061 tctaagactc tgatcgtcac ccaatcatac aacaacatcg gagtgacacc cagcagtcca
     6121 gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc ccatcctttg ccaaatatct
     6181 tgccaactca tgccattagc aatatttgcg acttgatttg ccacaaagac taacacggag
     6241 ccgaaaaaaa tcagttttaa aatcagacta tgttccttta tccattgcag catcaagtat
     6301 cgtttatttt tcacattttc ccattcctaa cttgtatact tttgtgtcat accccaatgt
     6361 tatcttattt attttaattt ttccacttta gacctgttct ttgcttgcga tgatagaaaa
     6421 agaaagatat cctaatgcta aaggagagga taacatgaaa gtaggattta tcggcacagg
     6481 gaatatggca caagctatta ttgatggttt attaaaaaaa ggaacagtct cgaaagaaga
     6541 ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa tttgctgctg aaactggcgt
     6601 ccatgcatgc aaaagtaatc aggaagttgc atcacaatca gatatcgttc ttttagccgt
     6661 gaaaccaaaa atcattgaaa aagtattgga agaactaaga gaatatagcc agaaagtctt
     6721 attcatttct attgcttcag gaaaatcatt aacagattta gaaacagcgc ttgaagatcc
     6781 tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc agcgtaggag caggcgtttc
     6841 tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt cagcaagcta cgatgatttt
     6901 tgaagcaatc ggcagcgtct atcctttagc agaatcagat ttcagtacat tcattggcct
     6961 tgctggcagc tcacccgcat tcgtttacat gctgatcgat gcgatgggac ggactggtgt
     7021 actgaatggt ttgtccaaac agacagccac tgagattgct gcaaaagcaa ttatgggttc
     7081 ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa cttgtagacc aagtttcttc
     7141 tcctggcggt acaactgttg caggtgtagt tgccttagaa gaagcaggat tcatcccagc
     7201 aatcatcaaa ggaatcaata cgacgatcga gaaagatctt gagatgttga ataaaaaata
     7261 atgatagcaa tgttttttcg tgccttacaa aaaaagaaga gctgtggctt ttgccgcggc
     7321 tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc cgaataacga
     7381 ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga atatcacgta
     7441 ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc cgaacttcgc
     7501 gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa atattttcca
     7561 gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca ggtcttgctg
     7621 ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct tccacatctc
     7681 catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg gcagctaatt
     7741 ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc ccaatatcat
     7801 ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct gctaagatac
     7861 cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa ctagttctga
     7921 atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca ccgacttcaa
     7981 acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga gatttctcga
     8041 ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt tccaaggtca
     8101 tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct tccggtgtat
     8161 catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta cgaccttctc
     8221 gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa acagtcgttt
     8281 ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt ttcgctttgc
     8341 gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt aactcatgat
     8401 tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt gataaaccag
     8461 caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct ttttctcgct
     8521 cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt gagttctcgc
     8581 gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc aaacgattct
     8641 cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca gaacggtatt
     8701 tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct gcttcttttt
     8761 ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct ttctcgtgac
     8821 gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg atagcgagga
     8881 gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta tcatctatac
     8941 agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat atgtgtacaa
     9001 aaaacatgca caacttt
//
LOCUS       DACDOA010000101         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDOA010000101 DACDOA010000000
VERSION     DACDOA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.101"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..877
                     /gene="purR"
                     /locus_tag="IWI39_002294"
     CDS             59..877
                     /gene="purR"
                     /locus_tag="IWI39_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9209354.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1380..2753
                     /gene="glmU"
                     /locus_tag="IWI39_002295"
     CDS             1380..2753
                     /gene="glmU"
                     /locus_tag="IWI39_002295"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9209355.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2847..3818
                     /locus_tag="IWI39_002296"
     CDS             2847..3818
                     /locus_tag="IWI39_002296"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9209356.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3824..3926
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3992..4561
                     /gene="thiT"
                     /locus_tag="IWI39_002297"
     CDS             3992..4561
                     /gene="thiT"
                     /locus_tag="IWI39_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9209357.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4642..5415)
                     /locus_tag="IWI39_002298"
     CDS             complement(4642..5415)
                     /locus_tag="IWI39_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209358.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5682..7382
                     /locus_tag="IWI39_002299"
     CDS             5682..7382
                     /locus_tag="IWI39_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9209359.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7352..7570
                     /locus_tag="IWI39_002300"
     CDS             7352..7570
                     /locus_tag="IWI39_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209360.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7590..8480
                     /locus_tag="IWI39_002301"
     CDS             7590..8480
                     /locus_tag="IWI39_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9209361.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8498..8782
                     /locus_tag="IWI39_002302"
     CDS             8498..8782
                     /locus_tag="IWI39_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209362.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3018 a         1530 c         1885 g         2509 t
ORIGIN      
        1 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
       61 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
      121 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
      181 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
      241 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
      301 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
      361 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
      421 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
      481 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
      541 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
      601 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
      661 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
      721 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
      781 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
      841 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agctgcttag
      901 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
      961 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
     1021 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
     1081 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
     1141 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
     1201 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
     1261 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
     1321 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
     1381 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
     1441 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
     1501 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
     1561 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
     1621 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
     1681 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
     1741 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
     1801 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
     1861 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
     1921 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
     1981 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
     2041 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
     2101 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
     2161 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
     2221 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
     2281 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
     2341 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
     2401 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
     2461 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
     2521 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
     2581 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
     2641 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
     2701 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
     2761 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
     2821 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
     2881 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
     2941 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
     3001 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
     3061 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
     3121 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
     3181 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
     3241 ctttggacct tcatgcagtt caaatccaag gtttctttga tattccagtc gatcatttga
     3301 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
     3361 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
     3421 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
     3481 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
     3541 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
     3601 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
     3661 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
     3721 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
     3781 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
     3841 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
     3901 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
     3961 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
     4021 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
     4081 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
     4141 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
     4201 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
     4261 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
     4321 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
     4381 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc cttggctctt
     4441 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
     4501 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
     4561 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
     4621 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
     4681 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
     4741 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
     4801 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
     4861 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
     4921 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
     4981 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
     5041 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
     5101 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
     5161 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
     5221 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
     5281 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
     5341 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
     5401 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
     5461 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
     5521 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt tatttaaagg
     5581 aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac tcatgacctt
     5641 tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata aaaaaagttt
     5701 acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa ccccttccat
     5761 tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa aagcaatgtg
     5821 cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa agtagaggag
     5881 atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa aaattttttc
     5941 ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat
     6001 ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac
     6061 aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga ggagcgcacg
     6121 gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac tttgagatta
     6181 tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga gatatgaaaa
     6241 aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta ccagaaatgg
     6301 ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg acgaaaacag
     6361 cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa gacaatttgg
     6421 atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct actaaactgc
     6481 cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa ataccagatt
     6541 ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc ggaaaaaata
     6601 aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt aacaaaggag
     6661 caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc
     6721 cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc tttctgcttt
     6781 ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg aaaaaacgat
     6841 taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca agaacatact
     6901 ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct tttttcaaaa
     6961 gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta ccagagacat
     7021 gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc aatgaaatct
     7081 atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt atgccggatg
     7141 atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat cgaaaatacc
     7201 agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa
     7261 aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca ttactgatcc
     7321 tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat atgaaatcgt
     7381 agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt
     7441 cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag accatgaaaa
     7501 aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa aagcaataga
     7561 aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca
     7621 tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac cagcgttttc
     7681 tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc
     7741 ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg
     7801 gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt atcactcaat
     7861 tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt agtcggatgg
     7921 cggaacgctt cgcgctggac gaaaacttcg taacggtaga agaaaaaccg gaagagagca
     7981 tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat tctattgagg
     8041 cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat actgaagaat
     8101 tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa
     8161 taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag caacggttgt
     8221 tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg gatcgtctgg
     8281 cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta ggtcgggaat
     8341 tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc aaatcaaatg
     8401 caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa atcagagaac
     8461 aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg acgtaacgtt
     8521 ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa aaaacggctt
     8581 tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta ttttgatcaa
     8641 cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca cagaagaggt
     8701 agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg tagtgaatac
     8761 tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata aataatcaga
     8821 attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt gtgacaaaat
     8881 tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt ttctcggtat
     8941 ta
//
LOCUS       DACDOA010000102         8837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDOA010000102 DACDOA010000000
VERSION     DACDOA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.102"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWI39_002303"
     CDS             278..787
                     /locus_tag="IWI39_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9209363.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWI39_002304"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWI39_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9209364.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWI39_002305"
     CDS             2322..3302
                     /locus_tag="IWI39_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9209365.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWI39_002306"
     CDS             3424..4842
                     /locus_tag="IWI39_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9209366.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWI39_002307"
     CDS             4913..6382
                     /locus_tag="IWI39_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9209367.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWI39_002308"
     CDS             6392..6859
                     /locus_tag="IWI39_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9209368.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWI39_002309"
     CDS             6895..7767
                     /locus_tag="IWI39_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9209369.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8837
                     /locus_tag="IWI39_002310"
     CDS             7848..>8837
                     /locus_tag="IWI39_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9209370.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDS"
BASE COUNT         3040 a         1319 c         1781 g         2697 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagc
//
LOCUS       DACDOA010000103         8792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDOA010000103 DACDOA010000000
VERSION     DACDOA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.103"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            137..1198
                     /locus_tag="IWI39_002311"
     CDS             137..1198
                     /locus_tag="IWI39_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9209371.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1201..2097
                     /gene="murQ"
                     /locus_tag="IWI39_002312"
     CDS             1201..2097
                     /gene="murQ"
                     /locus_tag="IWI39_002312"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9209372.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2111..4093
                     /locus_tag="IWI39_002313"
     CDS             2111..4093
                     /locus_tag="IWI39_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9209373.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4158..4604)
                     /gene="nrdI"
                     /locus_tag="IWI39_002314"
     CDS             complement(4158..4604)
                     /gene="nrdI"
                     /locus_tag="IWI39_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9209374.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4815..6239
                     /locus_tag="IWI39_002315"
     CDS             4815..6239
                     /locus_tag="IWI39_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9209375.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6420..7193)
                     /locus_tag="IWI39_002316"
     CDS             complement(6420..7193)
                     /locus_tag="IWI39_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9209376.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7190..8095)
                     /locus_tag="IWI39_002317"
     CDS             complement(7190..8095)
                     /locus_tag="IWI39_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9209377.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8249..8569)
                     /locus_tag="IWI39_002318"
     CDS             complement(8249..8569)
                     /locus_tag="IWI39_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9209378.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2760 a         1579 c         1871 g         2582 t
ORIGIN      
        1 acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa
       61 agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca
      121 gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga
      181 gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat
      241 gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga
      301 agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc
      361 gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat
      421 ggattatcat atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt
      481 gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt
      541 ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga
      601 atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt
      661 accaggagat acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca
      721 tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt
      781 ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa
      841 agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt
      901 agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg
      961 ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat
     1021 tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga
     1081 tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt
     1141 gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa
     1201 atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga
     1261 ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg
     1321 gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag
     1381 tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt
     1441 gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt
     1501 ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat
     1561 gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt
     1621 atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc
     1681 ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat
     1741 ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca
     1801 ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt
     1861 aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc
     1921 tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt
     1981 gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa
     2041 gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg
     2101 ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa
     2161 aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat
     2221 gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct
     2281 tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt
     2341 agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag
     2401 tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga
     2461 aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga
     2521 catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg
     2581 gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca
     2641 acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta
     2701 tacaggtatc aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg
     2761 ggccgttgtg atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg
     2821 tgaagctctt gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt
     2881 gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac
     2941 accaactctt tcattgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc
     3001 aggtttcatt tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc
     3061 atttgccggc ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca
     3121 aattttgact ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc
     3181 aattcttgcg atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg
     3241 caaaaaagat aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg
     3301 aatcggcgaa ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc
     3361 ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat
     3421 cgctattgga ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg
     3481 ctacatcctt ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg
     3541 tattcctaaa gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga
     3601 agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt
     3661 tgctgacgga acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat
     3721 gatgggtgaa ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg
     3781 ggtagtcagc tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt
     3841 agaaatcttg cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt
     3901 gaaagtaaca gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga
     3961 agcgatcaaa gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg
     4021 tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga
     4081 tgtcaaagct taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt
     4141 cagcttcttc ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca
     4201 tcagatggtg ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca
     4261 tattgtttgg ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca
     4321 ccaagacaaa gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag
     4381 atttctgtat caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta
     4441 aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact
     4501 tcaggattga caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca
     4561 aaggcacgcg tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt
     4621 ctttcctttt agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact
     4681 aggcgctttg cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt
     4741 tattaattaa gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt
     4801 tggaggatgc ttacatggat acagagcaac agaaaaaagg aattggttta attccacttg
     4861 ctgccttagt aattggttca gcgattggag gtggcgtatt tggtattatg acggacatct
     4921 ccggatcggc tggcggccca gctattttca gttgggtact cgtaggaaca gcaatgttga
     4981 tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa ggcgggatct
     5041 ttagttacgc agaagaaggt tttggccgtt tcgctggttt tatcagcggt tggggttact
     5101 ggctcacatg ctggttaggc aacgtagctt ttgcaacatt gttgatgagc gcattaggat
     5161 atttcttccc agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg agtacagtct
     5221 ttttatgggg atatgcatgg ctagtgaatc ggggagtcga aaatgctagt ttcgtcaatg
     5281 ctatcgtcac attctgtaaa ttagtgccat tatttttatt tattgtagta gctatcagca
     5341 cgttttcgat ccaacaattc attgataact tttctagcga cattatattg aatgcgaatg
     5401 gacagccgat cccgttttcc agtcaagtat tgagttgcat catggtgatg ctttgggtat
     5461 ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa tcagatgttg
     5521 ggaaagcaac gattctaggg atattcggct tgattattat ttacgtcttg gtttccatgc
     5581 ttccatatgg gacaatgacg atgaatcagc tgcaaacgat caaggcgcag cctgctatgg
     5641 gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac ggcggtttga
     5701 tcatttcttt agttggtgtt tggctctcat ggacgatctt accgattgaa acaatgggaa
     5761 acatggctga agacggactt ttgcctaaag catggggaaa actcaacaaa aatggggcac
     5821 caacttatgc tatcgtattg acgacgttat gtacgaatgt ctttttactt tcattattat
     5881 tcactgacag tgcttataac tttgcttaca cacttggtac agcagctatt ttcttcacgt
     5941 ggcttttctt aggattgtac caaatgaaac tttcttatca gcggaaagaa tgggtccaat
     6001 ttttagtagg gcttttagca agtgcctttc aagtatgggc tatgctagtc gtagccttta
     6061 atgaagtaat gcttgttctt gttcttttct tgccaggcat ttatttttac ctcaaagcac
     6121 gtaaggagca aaacgagcct gtacaacata tccacttaga taagatttta cttataggtg
     6181 tgacgatggt cggagtgatt gcaatggttt tatttgccac tgggatgctt cacgtataga
     6241 agtataaaag aagtataaag agaggttgtg agcctgtcgt ttattcaaaa acatactagg
     6301 aaaggagctg tgacacaaat gttgtcacag ctcctttttc agaataaacg gtgtttaaga
     6361 gacagccaat cggcaataag gctgaacgtc tctttcacag cctctttttt gatgagtcat
     6421 catacatttt ttcgcttaaa tacgagatat cctaatgcta agaacaaaac gatgtaagct
     6481 aaattaccga atagcatttg attagtagaa agatgagtca attgcttcat gatctcttcg
     6541 tttccaattt ggttttgcag attcaacata ctgatcggat tccatttgat ccactcccat
     6601 ttttggatga gtgcaataag gataccagat atgattgacg aagcaaaata aaagacgatt
     6661 cctgctgaga tggatgcgcc ggaactattg ataaagcaag caatcatcaa cactaaactt
     6721 aaaatcagcc ataaaccgat ataactaccc aaagtataag tgaacaagct ttggatcagt
     6781 gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat cagtttcatc
     6841 aacacagtga caataatagc taaaaggtat aaataaacag aataaataac taatgtgatc
     6901 catttgctta ctaatatttc tcctcgagaa tactttcgat aaaggagatt tttcaatgta
     6961 ccatattgtg cttccatcga aatgatcgtg cttgcagctg caatcatgat aaagacaatc
     7021 caagatgtgg cactaaaaag ctgagcagtc atttcaactg gatcaatgat cgtcgtgtat
     7081 tttttggcta gaagtgctgt tcctatgagc aaaacaacta atagaacaga taaaacagca
     7141 gttgactttt tcttgatcaa tttaaataat tcttgtttta caagcaatgt catttttctt
     7201 cttccccctt tactgaatcc agaatttcca atagagaaga ttccaaatca ttcgtcagat
     7261 gctggacgtc tgtcacatgg atattttgtg cagacaatag ctggatcacg gttcctaata
     7321 attcttcgtt ttcttttaaa agcttgattt tatcaggatc ttccaaagta tatccagctt
     7381 ctgtcaagat cgttcgagca gctagatcat ttgttgtaga gataacgatg aaatcttgcg
     7441 ctgtttggtt caattcagcc attgtacatt gtttgatgat ctttccttga ttgatcagga
     7501 tcagctcatc gaccattttt tcaagctcgc tgagtaagtg actggagatc aagaaagagg
     7561 tgccctgagt agccaagtcg gtaatgattt ttcgcacatc tcttgttgct tgtggatcca
     7621 aaccgttcat cggctcatct aaaataacga tagacggatg gttcagtaag gcaagagcaa
     7681 ttcccaattt ttgtttcatt cccaacgagt aggatttcgc ttttttgtga atgtactttt
     7741 ccatcttgag ctgattcacg atagtgtcga tagcagatac atcgttggtt tcagagaata
     7801 acttcagatg atcatagcct gttaagaatg gatagatacc cgggtattcg atcaatgctc
     7861 ctacttgttc caacccttta tgactagagg gagaaatctc attttcacca atacagattg
     7921 ttccatctga gtaatggatc agtcctaaaa tagctttcat gatcgttgtt tttccagcgc
     7981 cattaggacc tactaatcca acgattttgc caggggataa gctaaaactg acatcgctga
     8041 gggcttgttt gtgaccaaag ttcttggata attcttggac cgaaagactt ttcaaaaata
     8101 ttcactcctt ttctatagat agtacaagca tacacctgat gagtggagaa gtcgctaggt
     8161 cattggactt atttcttatt ttttcataaa aaaattgaat attaaataaa aaggcttatt
     8221 ttttctgatc agtacttatt ccaagtgtct agtggccgct agtaatcttg atgccaatga
     8281 tactgatgag tagcatcccc acaaagaacc aagtggaaag attcagctta tcatgaaaca
     8341 agaccacacc aattatgacc gaaccaactg cgccaatccc tgtccagata ggataagaga
     8401 gactcatcgg caaacttttg attgcctttg acaaaaagta aaaactagca atcatgccaa
     8461 tcactgtgta aaaagaataa ttgattttag aaaaaccatc actccatttc atcatcgttg
     8521 accagaatac ttccaaaatt cctgctatga ttaattccat ccatgccata cttacattcc
     8581 tccttaacta taaaaaaata cacaaaaaga ggaaaatcat cccttcaaag acctaatgga
     8641 atgatcatcc tctttatcct acccggtggc agattgtata tctttttctg tagtatagca
     8701 cacctatcca gatatcggca atctcatact ttttagtaaa gtgtaccaag cactgacgga
     8761 tatatcgtta gcggaaaaga agatgaatgc tt
//
LOCUS       DACDOA010000104         8746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDOA010000104 DACDOA010000000
VERSION     DACDOA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.104"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..241)
                     /locus_tag="IWI39_002319"
     CDS             complement(<1..241)
                     /locus_tag="IWI39_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9209379.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            513..857
                     /locus_tag="IWI39_002320"
     CDS             513..857
                     /locus_tag="IWI39_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9209380.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            858..1277
                     /locus_tag="IWI39_002321"
     CDS             858..1277
                     /locus_tag="IWI39_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9209381.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1369..1719)
                     /locus_tag="IWI39_002322"
     CDS             complement(1369..1719)
                     /locus_tag="IWI39_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209382.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1685..3016)
                     /locus_tag="IWI39_002323"
     CDS             complement(1685..3016)
                     /locus_tag="IWI39_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9209383.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3272..3838
                     /locus_tag="IWI39_002324"
     CDS             3272..3838
                     /locus_tag="IWI39_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9209384.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3983..4070
                     /locus_tag="IWI39_002325"
     tRNA            3983..4070
                     /locus_tag="IWI39_002325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4015..4017,aa:Ser,seq:cga)
     gene            complement(4122..5327)
                     /locus_tag="IWI39_002326"
     CDS             complement(4122..5327)
                     /locus_tag="IWI39_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209385.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5465..6353)
                     /locus_tag="IWI39_002327"
                     /pseudo
     CDS             complement(5465..6353)
                     /locus_tag="IWI39_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6440..6640)
                     /locus_tag="IWI39_002328"
     CDS             complement(6440..6640)
                     /locus_tag="IWI39_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209386.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6669..7091)
                     /locus_tag="IWI39_002329"
     CDS             complement(6669..7091)
                     /locus_tag="IWI39_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9209387.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(7108..7530)
                     /locus_tag="IWI39_002330"
     CDS             complement(7108..7530)
                     /locus_tag="IWI39_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209388.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7821..8012
                     /locus_tag="IWI39_002331"
     CDS             7821..8012
                     /locus_tag="IWI39_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209389.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            8023..8289
                     /locus_tag="IWI39_002332"
     CDS             8023..8289
                     /locus_tag="IWI39_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209390.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
BASE COUNT         2830 a         1471 c         1584 g         2861 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgtaa aataaaaaaa gcgttagcaa
      361 ctatgcttaa aaagcatatc gactgaataa actaagcatt ggacaggctg aaagactaca
      421 tttaaaatga aatgcgaaga aacaatagaa aataaagttg ccttagaacc atagttgaat
      481 cacctaacag tcgtgaaggg agaataagag aaatggcaga aaaacaaacc gcaggacgag
      541 atcaattagg agattttgca ccaaaatttg cagaattgaa tgatgatgtt ttatttggac
      601 aagtatggtc cagagaagaa ttatctgctc atcagcgtag tatcatcacc atatctgcat
      661 taatttcagg aggaaatttt gaacaattgc cagctcatct gaagattggc aagcaaaatg
      721 gcattactag tgaagaaata gcagaaatca ttacccattt agctttttat gtaggatggc
      781 caaaagcttg gtcagctttt agtttagcaa aagaaatcta tttagaagaa aatagcgaag
      841 ataagaaaga ggcgtaaatg acgacaaaac acgaagaaat acacaatggc gtgatttttc
      901 cagcaggtga aaaaaacgaa gcttacgctc aagtatttac cgggcaaagc tatctgaata
      961 tgttagtgaa tgatcccgaa gtttctgttg gtgtaggtaa tgtaattttt gaaccaggtt
     1021 gtcgcaatca ctggcatgtc cataaagacg gctatcaaat cttacttgtg accggaggag
     1081 aaggctggta tcaggaagaa ggaaagccag caagacgatt aatggctgga gatgtcgtcg
     1141 taacgcacaa agggattaaa cattggcatg gtgcgacaaa agacagttgg tttgaacatt
     1201 tggcgatcac tgctggtaat tcagagtttt tagaacctgt aacagatgaa gtgtatgaag
     1261 gattggaaaa tcaataatgt caagcttgaa cagctcctga aagtagattt taatcgaact
     1321 ttcaggagct gcttttttga ttcagtcttt tataaataga aaatagggtt aataatagga
     1381 atgagaagca cgtcgaaaga tcttaatatt gataatcggc tgggcataca tgatttccac
     1441 gtcatcaatc aaactaccta acagatgata atcttcccga tcatgtcggg gattacctaa
     1501 tagttcttga tagtagattt ccatactagg gtcccgagga ttgttcagca aatcagcaag
     1561 tacatctaaa tcttctggct gagtttctgc cgtaccctgt atctctatga aaagcccttc
     1621 ctcagcaaat tctactccaa tacgtatttc agttatttca tgggaatata aataagtaag
     1681 aagctcatcg atgattcttt ttgataattc tttttccaag tgtctttttt tcctttctga
     1741 cagcggtaaa gttatctagt atcggtacaa ggatacctgc aacgaaacca gttgaaaaac
     1801 cactgttata tagatttagt cctccgtgca aagtgctgac gttatggaca agagcaatat
     1861 gtagtacacc agctattaca ccgtaaaaac tgccgtaaaa accgcttatg ggcgcgagac
     1921 ctgtcccaaa cacagctgca acaagcatgc cagtactggt gatctcatga aaagttccaa
     1981 acaaagaagc aataaatatt ccgactagta tcggaaaact gttttttaaa tgacaaccaa
     2041 aagcactgaa acccaccgca gataaaatag caccgatgac aggtccattt aactgaccgc
     2101 caaccagcaa aacataagaa gacagcatca gaccaactaa gcctatgttg atcaaagtag
     2161 caccaatacc agaattagca atgaaatcag ttgtcaattt tccagatgaa tcgaaaattt
     2221 ctcgaatccc caaaagccga aaggaattat aatagaaacc aattccgact gtcagcgaaa
     2281 aaaagccaaa aataatccaa atgagaggaa agtgatattc attgaaaacc aatgtatttt
     2341 cgacgattga atagctgaat aacctcaaaa cagcagtaaa agtcatcgca atcaacccag
     2401 aggtgaatcc aatattataa agtgtgaatc cacgatggaa aactaaagtt tgagcagcta
     2461 agggcggaag aagtagacca attgctatgc cagctaaatt ccccaataaa atacctatca
     2521 gtaaaggaaa acgcatacca aaagtgatat aactaacgac cggtgataat gcgctaccaa
     2581 acagtccgat catgatgtac tgagaaaaag gtttattgac aaatacagcg tatagataca
     2641 cacctaaaat gattgagata gaattataaa ggtttttgcc aaaaaaagag aaaccggtaa
     2701 ctgttagaat cgaagcaaat aagggaccgt ttaaaatcac cttgtgccga taagctaaaa
     2761 gcacagaaag taaggtcaaa atccctacat ttatgaatgc gcttccgaaa ccgcctaaag
     2821 caaaataatc tgtcaaaaga ttgcttggcg aggtaagaat agttagcaat ccactccaat
     2881 tatcagtagg agtattagaa agcaagccaa taaaaagaaa aaaaacacct gtaaaaccaa
     2941 gataaaaata ctttcttttc tgtgaaacga ttcgtttttg aggatgaata taaataacgt
     3001 cagtttcgtt ttccattaca caccttcttt ttcattttct ttaactataa cttgaaatga
     3061 aagatttgga caactatcat ttaggtcaaa actaaaaatg taagggaacc cattgaattt
     3121 aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag actgaaaaaa aatttaaaat
     3181 ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca
     3241 tatactgcag taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca
     3301 aattctaaaa gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag
     3361 aaatattgct aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat
     3421 ggaagattta aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt
     3481 ccctattgaa cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc
     3541 aaaaaagcat catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa
     3601 aatgcatgat ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa
     3661 tcaattatcg gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg
     3721 cttagcgtca ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact
     3781 gatcaaacgg acaattgact cgattttagc attagataat cctgaaataa tcagatagtg
     3841 ttctatcagt atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt
     3901 tgctaaaaaa agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa
     3961 aaagtttaaa aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat
     4021 ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatatcaa
     4081 cgattagaca cgatttcgat cgtgtctttt ttgttgtata tttagcctaa aatgtttttt
     4141 gactcccctt ttgactcccc ctgagtactg ttcttcataa atgctacaaa attattgatg
     4201 acttttttgt tttgagtttc agttacatga gtgtaaatat ttgaagtagt ttgaatgtct
     4261 gcatgcccta aacgttcctg aacatctttt aaagaagctc ctgattcaaa aaggagagag
     4321 gcatgagtat gtcgaaaacc atgagtagtg attcttttag tcaattctgg atgatgctta
     4381 tatatgacat taagccacga acgtgaaact gtagggttat agagaagacc atcatggtga
     4441 aaaattaagt tttccctttg gattgtaacg atcgttttgt tttttgtata atactttctc
     4501 aataaactga tcaaatcatt gtccagataa atttttcttt ttccagattt tgatttagga
     4561 gtatttacga tcaatcttcc ttgataacct cttgttacag ttttattgat attgagtgtt
     4621 ttgctcttaa agtcaatatc cgaccaggtc agagcaaaag cttctccttt tcttatacct
     4681 gtaaaagcca atagggaaaa gaataaatat ctttcatcat catcgtttct tattgcttct
     4741 aaaaatttga ttaattcttc tttagtataa aattcgatat ttttatcttc gatatctaga
     4801 gcttctccac gtggaataga aactaatttc ataggattgc tagttataat ttgtaaagaa
     4861 gcagcgtaat caaaaacatt agatgtgtaa ttttttattt tcttaaaaat tttaggatgg
     4921 ctatcggacc aagtgtttac tgcttcttga cagaaaaaaa cgtctatttt gtcaatgaac
     4981 ttatctccaa acacttttaa gatgtgtgtg tcaaaaattt gtttggtgga ggaccaagtg
     5041 ctttctttaa ctgtcttttt ataattttca aaccataagc cgtaaacata ttcgaatttc
     5101 ttgctctttt gagctgtaga agcttgcagt cctttttcct gtatatctgc ctccaatctt
     5161 tttaaagctc tctcagcggc taatggcgta ctaaaacctc gtctcgttgt ttttcttttt
     5221 tttcctgtta gaggatcaac tcctaagtac agactaaact gatatttttc ttcgcctttt
     5281 tttgttaagt gctttttaat tcgtttatca atttcttttt ttgccatctt tatctttcct
     5341 ttcgtacgtt tgttcggttg tacagcggat ttcgagatgg taaaataggg tacaacaaat
     5401 agacctactt taccgtagct ctttgcacat ttgcgttctt ggtcgggcgg caaatgtgct
     5461 ttttttattt cactcttagt tcttgtcctg gataaagcat gtagttgttt ggatccatac
     5521 cgtttaatgc aaacagttga tctaccgtaa ttccagctcg ttcagctatt tgtttggtcc
     5581 cttcaccagg ttgcaaagtc aaggtttcgg tttcagatga agaaggtgcc gttggttgag
     5641 cttgattagc tggtacataa tcttgtggta ctgattgctc cgttgaactg ggagtgggtg
     5701 aaacttgttc aacacttgaa ctaggagtag cttctgcata gttttctaca ctttcagcag
     5761 agtaagtact actagtttca ggaggcgtat tctgcgcaat agcagcttgt tctacagtac
     5821 tagaagatgc aatagtactt tctgttgtag tcgaagatga ttgttcgttg ctacttgttg
     5881 agctagtagt tgttctagat tctttagttt ttttcctttt tgtagggttt caaaactaat
     5941 ttctcattat catcagattt gttaggcttt tctggagtta aaataatatt tttatcttct
     6001 ttgctgacag tatatttggc atcattatct ttgtctttat catctttcca agtcataaca
     6061 tcgccttcaa gtgtatactc aaatttgtaa ttcatttggt cgactaagct tttagcaaat
     6121 tcttctccca tagcctccca atcgttactt gaagttgatt tcatagaact tgtgtcaaca
     6181 ctaaaagata cgacgtgatc agaaaatgat acgatcatat taggttcgtc atctttggtt
     6241 gcttcaatca accaatcatt agcttttaaa tcttctgtag ttactttttt accacaagca
     6301 gtgaaaagta acagagaagc aaaaactaat aaacttgtta ttactttttt cattttttat
     6361 tcctcatttc tatgatatga tttttattgg ggaatcttag aaataaggtt tcgagtccgt
     6421 gttgctgcac gggctttttt tatttaaata aatcccaaaa actgaatgta gttttcttgt
     6481 atactttatt gtaagcagcc ttttttgggt ctttaatcca gccgctacct tttttcccat
     6541 aaccaggaat aactgctttt tttacttttc tttttgcttt tccagtggtt ctagcgctta
     6601 tggatttttt tatacttggt tttctcattc ctattttcat ctatatcctc cttacgttgt
     6661 attataattc acaagaaacg atcgaattca tctggtaatc caaagcagac gagcttttca
     6721 tattttgtca tatgatcaaa gccgtctaaa gttggatcaa tagtcagatg agttgcgaaa
     6781 taatttgctt ctttttcaat ttttaattca gatactagcg tttttttaga caacataggt
     6841 gtgttttcat tcggatgcaa aacacagtgg cctaattcat gactacaagt tatccatttt
     6901 cccatctcat ctaaactaga atttatgtgg atcattttta tctgtttgaa tttactatag
     6961 taaccataaa tttctcccaa atcttcttct aaaactagta tcttcaattc tcgtgcaatt
     7021 ctaaaaggat tgcgtgtttg atatagtttt accagcttat taattttgtt atcaatttga
     7081 ggtaaataca taaacaacgt actcctttta atcgcgatat tttttaggtg tgtatttttt
     7141 cttagcttct attttagcta ttcgcaaagc attctctaaa gaaataatga gagcttcacg
     7201 tgtcgcttca ctcatttcat tatctttctt cgaaaaagcc attccatcag cattagataa
     7261 atcgtcgatt aatgactgca attctttttg gatatcacgc tcgtcttttt ctgttaaatc
     7321 atagtatctt tttttctcag tacgtcctag caaataatct acagatacgt cgaagtagtc
     7381 agcaatttct tgtaactttt cggcagttgg ctgttttcct gcgtttaaac tataaaagta
     7441 attctctccg tacttcaaat ctttagcgac ttgttgaagg tttttattct tgtttttcgc
     7501 tagttctttt attctttcaa atgttgtcat tccaacgttc ctttccaaca cgaaaaaata
     7561 atttacgaaa aagagtaaaa atagctttac aattttacga tatagagtta ttatttatcc
     7621 gtaagttaat ttgatagaaa aaagcaaact aaaaacacac cttatagcat aaagtttggc
     7681 gaccaagtgc aaataaaagg ttttgttata ggcttatttc tctatgtatt cattttactc
     7741 tatagtgtaa aaatgtcaat ggaaattata ttttttctat cattttaact tacaaattta
     7801 taaaaaaagg aagtgggtta atgtcaaact tggataacgg tcgtgcggcg atcaaagagt
     7861 ttatgaaaga gaatggaata actatggatg atttggctac tgcttatggt cacacgcgtg
     7921 tacgaatgca acaaattctt gatggacact ggagtggacc aaaggcaaac agaactattc
     7981 tagagatcat ccgtgattac agaattcgat aggaggcaag tcatgaatat tctaagcgaa
     8041 gagtttttga ctcgtttaag aattgcaatt gttgaagttg taaaggacgc acttagtcaa
     8101 ctttcaaaaa agaatttgtc agaaacacga tatttaaaaa agatcgaagc tagaaaatat
     8161 gtgggaggtg taaacgatca aggttttgag aagttaatag ctcacggttt aaaagaaatt
     8221 cgtatagatg gctttttaag atatgacaaa aaggacatcg atgaactgat ggctaaatac
     8281 aaaatttaaa ggaggtcaac atgggaaagt ttaatcgaac attagtattc agcgcgccac
     8341 taattatcta cgctttaggt ctatggggta gtagacaagc attaatcggc acgatcgttt
     8401 acatgctttg gattttcatt gggcttgatg aagctgagta cagagcgaaa aagccagtcg
     8461 ggagggactg actaaaaaag tctaatgtct ataagaaagg tttgacaata aatgaaagaa
     8521 ttattttttt taataacttt catcattttt tctatcagtg ttttctacat taacctttgc
     8581 gttgctagat ttgctttgaa aagttttagg aacagcaaac gaaaatagag ctataaaaga
     8641 tacgaatgta gtagctacgt taaattgctc tttagaaaac gtcaatgtca ttggaatgat
     8701 aattgatatc gatgagaaaa tctttgcgat aataactatc catttt
//
LOCUS       DACDOA010000105         8721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDOA010000105 DACDOA010000000
VERSION     DACDOA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.105"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..1458
                     /locus_tag="IWI39_002333"
     CDS             4..1458
                     /locus_tag="IWI39_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP9209391.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="IWI39_002334"
     CDS             1534..2694
                     /locus_tag="IWI39_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP9209392.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="IWI39_002335"
     CDS             2807..3814
                     /locus_tag="IWI39_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9209393.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="IWI39_002336"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="IWI39_002336"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP9209394.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="IWI39_002337"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="IWI39_002337"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP9209395.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="IWI39_002338"
     CDS             5793..7388
                     /locus_tag="IWI39_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP9209396.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="IWI39_002339"
     CDS             7404..7718
                     /locus_tag="IWI39_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9209397.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8179..>8721
                     /locus_tag="IWI39_002340"
     CDS             8179..>8721
                     /locus_tag="IWI39_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9209398.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKH"
BASE COUNT         2646 a         1601 c         2060 g         2414 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
     8161 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
     8221 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
     8281 ccaaatggtg ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag
     8341 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
     8401 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
     8461 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
     8521 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
     8581 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
     8641 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
     8701 acgttaaaag aattgaaaca t
//
LOCUS       DACDOA010000106         8616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDOA010000106 DACDOA010000000
VERSION     DACDOA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.106"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(214..2595)
                     /locus_tag="IWI39_002341"
     CDS             complement(214..2595)
                     /locus_tag="IWI39_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9209399.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2585..3232)
                     /gene="recU"
                     /locus_tag="IWI39_002342"
     CDS             complement(2585..3232)
                     /gene="recU"
                     /locus_tag="IWI39_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9209400.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3296..3835
                     /locus_tag="IWI39_002343"
     CDS             3296..3835
                     /locus_tag="IWI39_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9209401.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3925..4344
                     /gene="gpsB"
                     /locus_tag="IWI39_002344"
     CDS             3925..4344
                     /gene="gpsB"
                     /locus_tag="IWI39_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9209402.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4366..4733
                     /gene="rnpB"
                     /locus_tag="IWI39_002345"
     ncRNA           4366..4733
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWI39_002345"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4942..6108
                     /locus_tag="IWI39_002346"
     CDS             4942..6108
                     /locus_tag="IWI39_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9209403.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6154..7650
                     /locus_tag="IWI39_002347"
     CDS             6154..7650
                     /locus_tag="IWI39_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9209404.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7731..8414
                     /locus_tag="IWI39_002348"
     CDS             7731..8414
                     /locus_tag="IWI39_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9209405.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2707 a         1566 c         1830 g         2513 t
ORIGIN      
        1 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
       61 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      121 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      181 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      241 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      301 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      361 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      421 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      481 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      541 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      601 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      661 agaggctacg tgtgacgatt ctgaagtgac aggcgtcatt tttttattgt aacctgtcca
      721 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      781 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
      841 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
      901 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
      961 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1021 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1081 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1141 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1201 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1261 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1321 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1381 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1441 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1501 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1561 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1621 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1681 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1741 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1801 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     1861 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     1921 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     1981 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2041 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2101 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2161 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2221 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2281 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2341 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2401 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2461 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2521 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2581 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2641 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2701 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2761 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     2821 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     2881 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     2941 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3001 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3061 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3121 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3181 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3241 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3301 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3361 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3421 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3481 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3541 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3601 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3661 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3721 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3781 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     3841 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     3901 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     3961 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4021 gacaatatca tcaaagatta tgaaacttac agcaaagaac tccttgcttt acaagaagaa
     4081 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4141 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4201 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4261 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4321 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4381 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4441 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4501 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4561 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4621 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4681 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4741 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4801 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     4861 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     4921 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     4981 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5041 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5101 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5161 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5221 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5281 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5341 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5401 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5461 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5521 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5581 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5641 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5701 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5761 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     5821 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     5881 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     5941 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6001 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6061 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6121 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6181 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6241 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6301 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6361 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6421 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6481 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6541 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6601 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6661 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6721 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6781 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     6841 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     6901 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     6961 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7021 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7081 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7141 gacaaatttt atcgttcatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7201 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7261 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7321 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7381 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7441 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7501 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7561 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7621 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7681 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7741 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7801 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     7861 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     7921 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     7981 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8041 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8101 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8161 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8221 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8281 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8341 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8401 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8461 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8521 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8581 ttgtgattcg gaattggcag gagaacaccg tttatt
//
LOCUS       DACDOA010000107         8570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDOA010000107 DACDOA010000000
VERSION     DACDOA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.107"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1768
                     /gene="yfcC"
                     /locus_tag="IWI39_002349"
     CDS             29..1768
                     /gene="yfcC"
                     /locus_tag="IWI39_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9209406.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1925..3109
                     /gene="iadA"
                     /locus_tag="IWI39_002350"
     CDS             1925..3109
                     /gene="iadA"
                     /locus_tag="IWI39_002350"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9209407.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3220..3423
                     /locus_tag="IWI39_002351"
     CDS             3220..3423
                     /locus_tag="IWI39_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9209408.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3524..4186)
                     /locus_tag="IWI39_002352"
     CDS             complement(3524..4186)
                     /locus_tag="IWI39_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209409.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4199..5701)
                     /locus_tag="IWI39_002353"
     CDS             complement(4199..5701)
                     /locus_tag="IWI39_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209410.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6249..7934
                     /locus_tag="IWI39_002354"
     CDS             6249..7934
                     /locus_tag="IWI39_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9209411.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKKVL"
     gene            8065..>8570
                     /locus_tag="IWI39_002355"
     CDS             8065..>8570
                     /locus_tag="IWI39_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209412.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT         2654 a         1538 c         1856 g         2522 t
ORIGIN      
        1 tactgaaaaa agaaaggaag aaataaattt gaagaaaaca aattcttcag gaaaaccgaa
       61 acgcgacttc actaaattat cgacacccca tacgtatgtc attatttttg gtgtagtcat
      121 ctttgcatgg atactgactt ttgtcgttcc agcaggaaag ttcagcacgc aagatattga
      181 atacaaagat gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag attccttccg
      241 ttatgcttac gagctggata agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca
      301 tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag tcattgctga
      361 cggagaaaaa aatcttacgc aggaaaaact agatgaaatc tccttgacag atgatgtgct
      421 ttacgatcag tacggcgaaa atatctatga tacttctaaa aaattgcata aaacagccaa
      481 aatatgggga acagatgatt ttggcggctt tggtttctta aattttgttt ttgaaggact
      541 agtatctgga gataagtatg gatctgctgt agggatcgct gcattgatcc ttgttgttgg
      601 aggtgctttt ggtattatta tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat
      661 cagtaaaact aagggactgg agcgtttagc tttaccgcta ttattctttg ctttttcatt
      721 cggtggcgct acattcggga tggcagaaga ggtaattcca ttctcaatgg tgatggttcc
      781 atttgtcatt gcgttaggat atgactcgat cgtagcagta accgtgactt atgtcgcatc
      841 tcaagtcgga aatgcgacct cttggatgag cccgtttagc gtagcggtgg ctcaagggat
      901 tgctggaatc cccgtgttgt ctggtgcgac attccgattg attatgtggg tagttgttac
      961 tgcattagcc gctggttata tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag
     1021 ttcgttgact tacaaatcag atgattattt ccgttctcat atcaaaaaga catctgatga
     1081 aaataaaaaa ttcatgcttg ggcataaatt gatcttatta gagatgttag ttgtccttgt
     1141 ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt
     1201 ctttgtgatg ggattagttg ctggaatcat tgctgttctt ttcaaattag atggaatgaa
     1261 agtcaatgac atcgcttctt ctttccaatc tggtgcagct gatttagctg gtacagcaat
     1321 cgtagtaggt atggccaaag ggattcttct tgtcttggga ggatctgatg catctgttcc
     1381 ttcagctttg aatacgattt tgcatgggat cgggacggcg ttgaccggtg ttccagctgt
     1441 cattggtgct tgggcgatgt acatcttcca aagtttgttc aacttggttg ttacatcaaa
     1501 ttcaggacaa gcagctttga ctatgccaat catggctcca ctggcagatt tagtaggtgt
     1561 atccagacaa gtagcagttc ttgcttatca gcttggttca ggttttatgg acgcttttac
     1621 acccgtatca gccagcttga ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg
     1681 ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat
     1741 gattgccatt gcaataggtc tgcagtagta ctggagacct gtaaataaaa aagtaatcca
     1801 aacagtagat aaggactcca agaaccgtca ttttacggta aaatcaaagg agtaaagatc
     1861 ctatcgtttg gattattttt taagatccta tcatcagtta tacttaaaga aagaggaaaa
     1921 atgtatgaaa atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga
     1981 tgtactgatt gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga
     2041 aggagtggaa gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga
     2101 ctgtcatttc catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga
     2161 agtcacattg agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac
     2221 agacggagaa ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga
     2281 aggaatctcc acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga
     2341 ttctatcatc aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt
     2401 atccgatcac cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc
     2461 tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg
     2521 aaaaagaaag ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca
     2581 atttttgcct actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc
     2641 caaacgtggt ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac
     2701 agatggtgaa gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt
     2761 agataatttc acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa
     2821 tcatttccta gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc
     2881 tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc
     2941 acgtatattg aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg
     3001 tatcttggat aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt
     3061 ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct
     3121 aactatcttt tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct
     3181 gaaggtagat caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt
     3241 tctaaagata tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca
     3301 gacgacaaga gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa
     3361 aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa
     3421 taaaaaccac ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag
     3481 acagatatgg ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt
     3541 gaaatttttt attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat
     3601 gagtcacgat gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga
     3661 tatcttgtga agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat
     3721 gttcacagca gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg
     3781 tcacctgttt ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa
     3841 cttgtcgata attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta
     3901 gagccgataa ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac
     3961 gataacttct taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt
     4021 ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc
     4081 ctaaaatagc gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat
     4141 cttgcggatc ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt
     4201 agtttaatag gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag
     4261 aaaacagact gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat
     4321 tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt
     4381 gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag
     4441 cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat
     4501 tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca
     4561 cttcggtaaa caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc
     4621 ctaactcatg ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac
     4681 cggctacagc cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt
     4741 ccaacattga ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca
     4801 gcttctcagc ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag
     4861 cagaatctat cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag
     4921 tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt
     4981 cgctggattc ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac
     5041 tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa
     5101 tgactacatt gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg
     5161 ctgttccatc agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt
     5221 ctgcttgagt catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag
     5281 tagtttctga actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg
     5341 ccatagctga ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt
     5401 tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt
     5461 cagtggtaga accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg
     5521 cacttaaagt cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag
     5581 cgcttcgaat cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca
     5641 ataacaatga acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca
     5701 tattttgcat ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct
     5761 taaatcttat gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta
     5821 tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa
     5881 atgaaagagg aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt
     5941 tgttctaaca attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca
     6001 gctgaatact tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat
     6061 gaagtatatc caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt
     6121 tcctgctgat tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc
     6181 tcatatattt agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg
     6241 tgaagtttgt gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa
     6301 acatgtatgt cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc
     6361 ctgaaaatga gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa
     6421 acattgatcg tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac
     6481 ttccttactt gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt
     6541 tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg
     6601 tagatgagca tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc
     6661 atacgatacc agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata
     6721 cgggagattt taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat
     6781 tggcggaaat cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa
     6841 atcctcagcc tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat
     6901 attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag
     6961 tattggatgc agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac
     7021 gaattattcg cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg
     7081 ttacgttgaa agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc
     7141 gaatgggtga acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca
     7201 agattgaaga aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca
     7261 tcgtggcgaa aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg
     7321 agaatatgcg cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga
     7381 tgaaaccaac atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag
     7441 atttggctca tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg
     7501 tgctggaata taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa
     7561 tgattgatgg attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct
     7621 tgtcagaaga cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca
     7681 tttcaaaacc tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga
     7741 tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt
     7801 ttgagtggag caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac
     7861 aaacaaaacg ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata
     7921 aaaaggttct ataatatttt gtgttggtgg aaaatcgaaa gcgattttct actaacactt
     7981 ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc gaccaaaacg
     8041 tcaccctacc aaaggagtct ctcaatggat aatcatacta gaaaattact cggattaaca
     8101 gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa aggcgttcaa
     8161 gcgcagttta ttaaagggag attggatgac tcaccttccc attgtgaaca ctgtggatcg
     8221 attcgtatta tccgcaacgg ttcctatacg acacgcactc agatgttgaa agtcaaagaa
     8281 aagttgacta ttttagaatt aaaacgtacc cgttttctct gccatgattg tggacggacc
     8341 ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga actgaagcag
     8401 gcgattctca tggaactgta cgaaaatcaa tcacgtaaac taatcgctaa gaagtacttc
     8461 gtctcggatg ggacggtgac acggatcttg cgtgaagcaa cgaagcatta ccagccacgt
     8521 atgaacttcc ttccgacggt gttgtgtatg gatgagttca aatcaatgaa
//
LOCUS       DACDOA010000108         8492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDOA010000108 DACDOA010000000
VERSION     DACDOA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.108"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..172)
                     /locus_tag="IWI39_002356"
     CDS             complement(<1..172)
                     /locus_tag="IWI39_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209413.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(592..1713)
                     /locus_tag="IWI39_002357"
     CDS             complement(592..1713)
                     /locus_tag="IWI39_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9209414.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2041..3450
                     /locus_tag="IWI39_002358"
     CDS             2041..3450
                     /locus_tag="IWI39_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9209415.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3447..4175
                     /locus_tag="IWI39_002359"
     CDS             3447..4175
                     /locus_tag="IWI39_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9209416.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4303..5214)
                     /locus_tag="IWI39_002360"
     CDS             complement(4303..5214)
                     /locus_tag="IWI39_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9209417.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5231..6238)
                     /locus_tag="IWI39_002361"
     CDS             complement(5231..6238)
                     /locus_tag="IWI39_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9209418.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6235..8364)
                     /locus_tag="IWI39_002362"
     CDS             complement(6235..8364)
                     /locus_tag="IWI39_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9209419.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8369..8476)
                     /locus_tag="IWI39_002363"
     CDS             complement(8369..8476)
                     /locus_tag="IWI39_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209420.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1486 g         2680 t
ORIGIN      
        1 ttaaaaagtt agtaaataat tgccctctaa gttccacggt ctgttcatta ataactttat
       61 atcctcgttt ttcaaaaaat ggctttgcgg taatggaagc gtgggtagtg attttttgca
      121 taccagtttc taaaacatgg ttttccaatt catctgcaat tttagtcgca ataccttttc
      181 cttgatggct atgtttttgt cacaacttca gtattcctgt aacaatacgg atttcagtat
      241 acaacaacag cttttgaaaa gatacttgca aaaggattaa gatatctatg acctgctatg
      301 ctcactgatg ttgaaaagaa tgttttatta ggattgaggt aagcaagata gtgaatatgt
      361 ccggaaagcc tgtaaaataa ggcttttcgg acatatgtga agttataaaa agatagcgaa
      421 aaatctattt agtatcacta atgttgcgaa catatcgtca gaatagtctg acatttattt
      481 cttgctaaaa atttcaaaaa agtcaagtgc tgacgcagcg gatgactatt ttccagaaaa
      541 ttttagtatg tattcaaaaa tcaaacgtga caacagctat taactacatc ctcatccagg
      601 tctgtaacgt ttgaaccact cttctgcggg ctcaaacatt aagtaatgga aggtacgttt
      661 caattgaaac agcgtcgctg tcgtattcaa ctcaattttt atcacataag tgagaagcgt
      721 tgcgattaag gtgaggatga cttgatttat cgccccttgt tcactatgtg aatatagtcg
      781 tttaatggtc agattttgtt ttaagtgttt gaacaacaat tcaatctgcc aacgggcttg
      841 atacatgtcg gccacttcgt ttggcgatac atcaaagaga ttggtcacca ttcgcaggat
      901 tttttgtccc ttggtaagaa ccgttaccaa cctaaaacgc tctgtcacat ggttgacacc
      961 gccgacaaga atcacttgtt ggtcactgat tatcgtccca tcacgggttg ttgtatctgc
     1021 tacttcaatt tgatccaaga tggtaatttt tgtattagat tttgtccgtg taacgaagaa
     1081 atagccgtca cgatttagtt tgtctaacaa cttgtaatca aagtagccgc gatccaccac
     1141 atacgttgct tgcgttttat caacaagaac ttcaaggtga ttacggtcat gctcactcgc
     1201 atttgtcatc gtaaaacttt caggatactg attggtttta tcaagataac agagattcaa
     1261 atgtagcttg atgccagact ttgttttccg aaacttggcc catggaaacc atgctttgtt
     1321 caacggaata gtcgttgaat caatcaattg tagactggtt gtttttgagc ttggctgctg
     1381 agcagaaatt ctggcgacta attgcgtaaa aatttccatc aatacttctt gcttgatttc
     1441 tggcgctcta cgagataacg agctgtaaca cagggaatca atgccaagtt ccttgcagag
     1501 gcgctgatct aaaaaggcgc gatcaatctc gcgatagctt ggcaattctt cataaaccgc
     1561 atgaagtaac actttaagcg tcgtttggaa gtctagtttt ttactgtaga aatcaaagtc
     1621 gctaattaag ctttgagagt tttctgataa accagaaaaa tttattgcag aaaaccattt
     1681 ttgaaatgag gtttttacgt tatacttatc catgcgtgag tcctttttat tggtcttgga
     1741 taagtcatcc gagaatagta taaaggactt ttttgcgttt ggatagcttt gtattaggct
     1801 tttgatgggt taaaaagtct ttgcaacact agtaatttag tatttatgta taaattgaaa
     1861 gatattttac caccacttag tcttcttaaa agtgatccaa cattttgttt gtggcagaat
     1921 ttaactacac actaatgcag ccgaaagtac acaactatgc agcagtttga ctgcgaactg
     1981 gtttccttcc atcataagtt tgaatgactt agtgatggag gtacagaaag gttgttaact
     2041 ttggatgata ttaaaacaat aaaacatttg agaaataatc gtgatcagtc aattgatcag
     2101 ataagaaaag caatgaatat caattggcga acagcgaaaa aatacgccga tagtgatcaa
     2161 ctattcatta cagatcccac tcgaaaaaac aaatccatga tggatgatgg ttggggagaa
     2221 atcatcggta gttggctaga agaagaccta cgtttacgca gaaaacttcg tagaacggcc
     2281 aaagctatcc atgaacaatt gaaacctttg ggtttccctg gctcctatcg aacagtttgt
     2341 gtatacattt ctcaatggaa aaagcagcgt tacgacgaaa cgaattacca agctgtcgat
     2401 cgtcttgatc atccgatcgg agaagcacag gtcgattttg gaacaatgga agttatgcat
     2461 gaagatcaca tgaaagatat acaacttctt atcatgtcat ttccctatag taatgcgacg
     2521 tccgcaagtg ccatgccagc tcaaaaccaa gagtgtttcc tggaagcatt aaaacaacta
     2581 tttaatcaat gcggcggcgt tccaaaaacg atcagaatag ataacttaac tccagcggtg
     2641 aaaaaaacaa aatcaaaatt tgaagaagca caactaaccg atgaattctt acgtttcacc
     2701 attcattatg gttgtgatgt tcaagtctgt aacccacgta gtgggcatga aaaaggacat
     2761 gtcgaaaata aagtgggtta tgtccgatac aatttcttct cgacttcgcc aatcatggac
     2821 tcttttgagt ccctaaataa gcaacttgaa gaacaattaa aagcagatcg aaaaaggacg
     2881 cattattcaa aacatcaatt aatcgaagat ttgtggaaga aagaaaataa agcgttacat
     2941 tctcttccac aagaggatta cccaattttt aaagaagtgg aagctaaact aaatcgttat
     3001 cacgaaatga aattggatca aacggttatt catattccta aagcatatgg ctattctcaa
     3061 ttgactgttg ttttgacttg gaataaattc aaagttctta cgcctcatgg agaaatatta
     3121 atgaacgaat atcgccccta tatgaacaaa aaaagattga tcccatggag tgacatcatg
     3181 aaacaatgga gtaataaatt gaatgtcgtt agttattcga ggtattggaa gtacttgcct
     3241 gcacgccttc aaaattatct agcgattgat aataatcacc tgaaatacag acggataaaa
     3301 caattactaa gtttattagc gacctacgat atccaacaaa ttaatgaaca attttacgaa
     3361 ttagtcgttc aagaagacgc cccctctgac tttgatgtga attggtcaaa ctatgatgca
     3421 ttaacaaaaa caggaggtgt gcaaaaatga ttggacagac gttagaagat tattgtaaaa
     3481 agcttcgatt aagccatatc atcacgaaca taccaagagc ttctttcgaa gctccagaac
     3541 aatttattac cgatttattc cgtttagaac tggagaccag acagcagatc aaattagaac
     3601 ggatggaaaa attagccaaa tttcctgtac caaaatcgct aaagaactat gaatggcacg
     3661 aagatatttt tctaccatct catctagata aggagaccct tgtctctctt gattttctaa
     3721 aaaggcaaga aaacattgtt tgtgttggct ctcctggtac agggaaaaca catctagcga
     3781 tcgcattagg gagaaaagcc tgtagtgaag gtgtttcaac caaatttttc cgagttaaca
     3841 atctcgtaga gcaattagag aatgcattaa aaaaacaaca gctagccagt ttcaagaagc
     3901 gattcgaaac aacagaatta attattcttg atgaaatggg gtacttacca ttgacaaaag
     3961 aaggagctga acttctcttc caactgattt cagattggta tgagcaaaag agcattatta
     4021 ttacctctaa tttagaattc agtcaatgga atcgagtgtt tatggattct cgattaacag
     4081 ctgctctcgt tgatcggcta atccatcacg ctcacattat cagcttcaaa ggcgaaagtt
     4141 tcagattgaa aaattcatta tctaaatcat attgaaggaa aaaactagtt gcagcaaaac
     4201 tctgcacttt cggctgcatt tttttgtaaa aagctattgc acaacacaaa catttgaacg
     4261 acgtaattcc aaatgttgga ttgcttttag ataatctttt atttattcac aattgctaat
     4321 agtccaattt tcatttttgt atagttttaa tctatactga aaaacacttg ttgttttgga
     4381 caactcatca agatactcaa caccaacaat cacttgaatc gctttaccct tttgtttgta
     4441 aataggattc actaattcca caaatttcaa attagtatta attggttgca tcgcgtcttt
     4501 ttcaacataa tgctctagtt ctttttctga ggctgctgga taaagcctga aaaacgtctc
     4561 taaaaactcg gtgatttctt ccgttgtctt tgaatcaatt tttgaatcac tttttacttg
     4621 tcttacttta tgttttgcct tttctggctt accaatcata gtgggtaaac tcgttacaat
     4681 atgattgcct ttttcatctt caaagatcat cagacgatat gcagaagaaa ttgattgtgt
     4741 ctttttccct tctgtaatct tttgatctac cacaaatgaa acgacaaatt cttccgagtg
     4801 tttttcaaca tctagaattt ttactgatac aacctcggaa ctagtgggaa tatccgctcg
     4861 aaccatatct tggctcaaag cgagcccttc ttctgtaagg tactgctcaa gattagtcat
     4921 tctcttttca attacctcct tgttattctt ccatgaaaaa tattctttca caaaatctac
     4981 agtaaaattt tctactccag aggtattcac tattttctct ttcacgacca ctttctcatg
     5041 agttgtatgc tgatcaatgg ccgtgaagtt tttgtagatt ccaaaagcaa ggctacaaga
     5101 caataaaagc caaagggcaa gcaccatttt tcgatgcttg cccacggata ctactcgttc
     5161 tttcttcttt tttggaatct tttccttgtt acttcgctca atttttatct tcattttcca
     5221 ttcctctttt ctattgttta taccgaccag caccaataat gtgctgttgc caatagctat
     5281 cggtaagatt ggtccaaccc accggatctc ctgcatgaaa cattctgttc tctcctgcat
     5341 aaatgcccac atgagtcaca taagtaccgg catcataagt tgaatgaaag aaaatcaaat
     5401 cgcctggttt ggcatctttt aagtcaatat gagtcatagc atcatattga tcctgagcca
     5461 cccgagggag tttcaaacca actttggcat aacaccattg agttaaacca ctacaatcaa
     5521 acccagttcc gggacttgac ccaccaaata cataagccgt tccttcatac ttgaacgcct
     5581 cgtcaaaaat tccttggact gttttatcat caaactgtgt tgtagttagg tactgcttca
     5641 ctaataagca ataaaacata ttgccatagt tataccgcca cccactattt tccttaatcg
     5701 cgatgggatt gggataatcg actttttgac cacccgattt ttcttttgaa aaagattcgg
     5761 ctaaagcaaa actatgcttc tttccgtttt tcgcgacata atccaaatac ccgtcaccat
     5821 agttataagc ctgaatgata ctatcttgat cgcatcctag ttctttggaa cacttaacca
     5881 aagacgaaaa atagagacac acttgtttga tggatgcctc ggtacttaat gaattcggtg
     5941 ataaacctgc tgattcagaa ctttgcatga catcttccat tgtacctcca gattcgactt
     6001 gaataatcgc caaaagaatc gaaacatact ctgttatatc tgattctttg gcatactttt
     6061 ccaccattga acggtgtttc aaaactactt ctgagacact actacctcca ggagaagttc
     6121 cagaactacc acttccctca ccgtcctcac caaacaacaa acccacacac atcaagatgc
     6181 ccacaaaaga gatggtcagg aaggttaacg aaataatact caattttttt aagttcatct
     6241 tttctcacct ggctttggtt ttgggcgagt gttctttttt ctttgtactt gttgattggt
     6301 tcgctttgga aaagtgaatc tctgtttcac ttgttgaaag gaattgttct tgcttagtgg
     6361 ctgagtgctt ccgtttggaa tgacttcttg actagaaagt ttccgtttga tttcaggatt
     6421 cgaatgtact tgtacccttg gttttaaaga cctattttct ggtagttcat ttttctgtgg
     6481 ttttatctcc ttttttatcg gaacattttc ccttcctttc tcatgacgat tccatgcttc
     6541 gttcaatact ttgcgttttt catccatttt tgcgtctctt cgttgtcgtt gtttttctcg
     6601 caactcccct cgatttgctt tttcttcgaa aggccctcgc ttaaagtcct ctggtacttt
     6661 tttcgtcttt tccatgcctt tatgaaggct gtatttcaag ttagttggtg catcggttac
     6721 ttgatttttt gcttgtttcg ctttgtcctt cacgatttct tttgtatcga gtacttttcc
     6781 aacttttcta ccaactaaat ttattcgtga ttctttcttc gtgcccactg gttgttcttg
     6841 ttttttcaga tcagaattta gctcgttaat gcttctcgcg gaatggttct ttctattgga
     6901 cactctattg atttttgaac tacctttccc ttttgtagaa ctggtagcta gtttcccacc
     6961 aactgctgca ccgccaccaa aagcaaaagt tcgacccaca tttcgattaa gctgtcgcaa
     7021 ttttcgattc atcaacattt gcggtttccg catgactcgc cttcccattg actgactatc
     7081 attactttga aggttaaaca tgctcataat gtcacctaat ttgaaataga taccggcaaa
     7141 ggtcacaatt tgtagaaaag ctaccataaa gaagggatag gtggccgaaa tattgaaaaa
     7201 catcgtggag atactgaatg cagtcgtaat gactaaggta actcccgctc gcatcataat
     7261 cgtattgaac aaacgaataa tcgccctttt gcccaaactc tcatacgtcg gcaacatcga
     7321 caataagaaa ctaatgggca gaaacatcgc aaagataatg aaaaggattt gtgagaacaa
     7381 cataatccct gtcagaagaa acacaaaaaa ggaaatccca atattaaaca acactaaaaa
     7441 gaccacggtt cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattatc
     7501 ttcaatctct gctttgactg cttcttcccg atccttaccc ttattttcat ccggactcac
     7561 ggataaaatc ttattaaccc gaccctcgcc aatttcttct atattggaat catcgaattg
     7621 taatagtaac cagggttgtt gaacttgaat cgaaaataaa ctgtctcgaa tcaaatccac
     7681 actatctttt ccctggcttt ctgaattagg tacaacaatc ttggttccaa ggctaagagc
     7741 cgcttcacta acgtctgagc taaagtcatt gatttttgta atataggtgg gcgcatacgc
     7801 aatgaaagaa cccgagagta gaaaaatcat cacaaaatta atgacggttc ggactgcttt
     7861 ggtagtttct cgttttaata atccggtata cgccacataa actcctagag ctaaaatcat
     7921 caataatagg aacccaaagt agaatcccga agtctgtaat ccattttcag tgataccggc
     7981 taaagtttgg atattctttc caatactttt agccgtatcc gaaatgaaat ccaacttgta
     8041 agcttcttga accacatagc ccgtggcgtt tgataaatac agacttactg tccaaataaa
     8101 attggtaatg gcataaagac catacatcac gcttttccct aagccatccc cccaattcca
     8161 aggtaaccaa tcccaagaac tatccacaaa gaaatccagt tgataattgt tcagagaata
     8221 tttggaataa aggtttccgg cttcaactgt atcatcaacc agacctgtcg cctcagctac
     8281 cgtaccgact aaactcaata gaactgtcag agtgacgatg ctaataaaaa cgattcccac
     8341 aatccgaaaa agtttctttt tcatcccctc actccattct ctcttgaacc ggcggacgag
     8401 tgtcaaaagc atggaagagt tcttcaaaga ctggatgaac ttgaaccaca cctactcgcc
     8461 catacaagtc ttgcatcaag cattgaccgt tt
//
LOCUS       DACDOA010000109         8351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDOA010000109 DACDOA010000000
VERSION     DACDOA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.109"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            252..1577
                     /locus_tag="IWI39_002364"
     CDS             252..1577
                     /locus_tag="IWI39_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9209421.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1570..1920
                     /locus_tag="IWI39_002365"
     CDS             1570..1920
                     /locus_tag="IWI39_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209422.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(2991..3266)
                     /locus_tag="IWI39_002366"
     CDS             complement(2991..3266)
                     /locus_tag="IWI39_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9209423.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3238..4191)
                     /locus_tag="IWI39_002367"
     CDS             complement(3238..4191)
                     /locus_tag="IWI39_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9209424.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4803..6296
                     /locus_tag="IWI39_002368"
     CDS             4803..6296
                     /locus_tag="IWI39_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9209425.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6431..6727
                     /locus_tag="IWI39_002369"
     CDS             6431..6727
                     /locus_tag="IWI39_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9209426.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6828..7679
                     /locus_tag="IWI39_002370"
     CDS             6828..7679
                     /locus_tag="IWI39_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209427.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7700..7912
                     /locus_tag="IWI39_002371"
     CDS             7700..7912
                     /locus_tag="IWI39_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209428.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2902 a         1293 c         1572 g         2584 t
ORIGIN      
        1 tcttttttga atttcttatt tttttctaat ataactaagc aaaactggat aatacgacac
       61 ttattttcaa attttcacaa gactaatagt aaatatccat ttaataaagt gatacaatgt
      121 gttgatttta caaatcaaca gaagaagtag ggaaataact tacctatgct tctttttttg
      181 tacatttaat cgattcttct ttacaaaaac gaacgtatgt ttgtataatg tggaagaatg
      241 gagtgttatt tatgatggat tattcaaaag agccagtaaa tgattacttt ttgatcgata
      301 tgaaaagctt ttacgcaagt gtcgaatgta tagaaaggaa tttagatcca ttaacgactg
      361 aattagtcgt catgagtcga gcggataata caggatcagg gttgatttta gcttcttcac
      421 cagctgcaaa gaagaagtac ggtatttcta atgtcagtcg accacgagat ctaccgcaac
      481 cttttcctaa aacgttacat gtcgttccac cacgcatgaa tctctatatt aagcgaaata
      541 tgcaggtaaa caatattttc agaaggtatg tagcagacga agatttattg atttactcga
      601 tcgatgaatc gatcttaaaa gtgaccagat caatgaatct ttttacgact gaaggaacaa
      661 gaagtcaacg gagaaagaag ctggctcaaa tgatccagga gaatattaaa gaagagctag
      721 gattgcttgc tacagtaggt gtcggagata atcccttatt agctaaactt gctttagaca
      781 atgaagcaaa acataatgat ggattccttg ctgaatggac gtatcaaaat gttcctgata
      841 aagtatggaa tattcctgaa atgacagacc tttggggtat tggatctcga atgaaaaaaa
      901 gactcaacca aattggtatc atgagtatta aagacttagc taattggaat ccatacacta
      961 ttaagagcca tttaggtgtg attggcttac agctgtattt tcatgcgaac gggatcgatc
     1021 gaacagatat agcgatccca ccagaaccga caaaggaaaa atcatatggt aattcacaag
     1081 tgttaccacg agattatacc aggagaaatg aaatcgaact ggtggtaaaa gaaatggcgg
     1141 aacaagtagc tattcgtatc aggcaacata actgtaaaac aggttgtgtc catttataca
     1201 ttggcacgtc gatcttagaa acgagaccag gtttttcaca ccagatgaag atccctataa
     1261 cagataatac aaaggaattg cagaattact gtcttttctt gtttgataag tattatgaag
     1321 gacaagaagt aaggcacgtt ggaataacct atagcaagct tgtgtacact gattcgcttc
     1381 agctggattt atttagtgat ccacaaaagc aaattgacga agaaaattta gataaaatta
     1441 ttgacaagat tcggcaaaaa tatggtttta cgtcgattgt acatgcttct tctatgttag
     1501 aaggcgctcg tagtattact agaagtacat tggttggtgg ccatgcaggc ggcgcaggag
     1561 ggcttaaaaa tgattgatta taatgatcgt ggacgtttaa agtgggcagg tttttattta
     1621 tctgatcata ctgaaaaaat tgatgccgac aatgaacaac gagccaatca aaatttggca
     1681 aaagaacaaa tgacgactga agaggttgcg gaagtattga acgctgcagt cataaaaaat
     1741 agatcagtat gtattcaaaa agaagaacga aacgctgaag gatactatcc agaagatatt
     1801 gttagcaaga tcttaggtag tgatgagctg gggatctacg ttggaaaaaa caaagttcac
     1861 tacgacgaaa tacgtcatat cgaattttac caggataata aatggggtcg attagaatga
     1921 acattatcca gcaatatgag ctaaagtata taacctttga tcagctatca gaagagattt
     1981 ggggttatgg ccagcgtttg ataaatgagg ttggtgttga acgcttctct ttctatgtag
     2041 aagctgcagc aggataccat aaatacgaat actatattgg ctattaaagt gcaataaaat
     2101 tatatttctt ttatgtttta cattttgtat caattctgat aaaattacct ataggtaatt
     2161 aaaaaatata aaaaaactag ttacaacctt aggacgttgt aactagcttg aagcatacta
     2221 gagctaacta aaatagcact agaaagcaat gttaaacccg tccaacaaac ttgatttaac
     2281 attgagacca gcttattcag ctaaatatag ttgaataagc ttcttttgtt ttaaaaaaac
     2341 aaaaggtgta ctacgaataa cgcaagtaaa agaaatacta ttacaaccag taggctacaa
     2401 gtaagtatta ctaaacattg cgtagttaga acctttgctc tattgtctaa agccacaaaa
     2461 ttagagatca gctttatgat gctaagagcg agctgcatcc atgcggacat aggttctttt
     2521 gacactatta attctccttt ttaggataag ggaaattgct agtagttttt tcattagcac
     2581 catccccctt catttgattg agtctagtgc tgctgtgata gtttgctgtt taatatgctt
     2641 acattcttat tataacataa atgaaacaaa cacatggtta gaggtcgtat caatcaaaat
     2701 agtgtctcac aaacgattta ttacttactc ctgctgacta acggactgtt cttcagtaac
     2761 tgtaactata tgtttgttaa aaccaccatt acctgtagat aaaaaccaat atatttacta
     2821 tactcactat attaaatata tttagtatag taaaaactag aaacaataaa atttgtgaat
     2881 gttgtatacc cccaaacaat acacatattt tgaaggatta ggattaataa agctaaaaat
     2941 atagtaagta tactaaatat atttactata tctagtatac ttactatatt ttaatctttt
     3001 gatcgtctat tatatataat ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt
     3061 gcaagtttga ctatttcata cataggaata tcttttgcat aactaagatc ccgaatggtt
     3121 tttagtacgg gaggatctac ttgaactggt tttctttctt tcgcagtgta ctttcgttcc
     3181 cctaactcga cattaatatc atctttaatt gaaatatctt cagcaccaat tttcttctta
     3241 tctttgttcg ttggtaataa tgccatttag tttaatctcc tttccaagag gtaggatatg
     3301 attggccatt gagtcagcat atttaggtgt ataatcaaat ccttcaatat caccatgttg
     3361 ttcatagtat tcaattcttt cttctaattc tgtaaagatg tctgcaaaaa tagcccacca
     3421 tcttcgatct acataatcat ttaattggat accattctca ccatacattt ctaaccgatc
     3481 cgagccttta acaatgcttt taaaaatgtt tgattcacca tacttatcaa ttgttttttg
     3541 atagtttgcg tgttgagagg aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat
     3601 ttgaagatga gactcttcaa aattcgtgac aatcggaact aaaactttat taataaaatc
     3661 ttctgttcct tccattgcgt accgttttaa ttcttgcatt gcaataatat aatctgcagc
     3721 agttaagaat gcacgaacca cattatctgt tgatggtggg cagtcgataa agatataatc
     3781 gaaatttttt ctcaattctt ctaaaggttt aatgaaggcc aatgatctct tacgacgatc
     3841 aggatatttt tcggacaagt attcattaag ttcttcagat ccaggagagc cagccataat
     3901 atataaatgt ggacttactt gagtaatacc tgaaacccag tccccttttt tgattgctgc
     3961 agacatggat cgatcaaagc tagtaatttg atacgttgat ccaatgttgg aagtagtatt
     4021 tctttgttgg tcataatcaa ttagcaacac gtctttttca aaaagttgac tagaaacaat
     4081 cgctaataaa tcattgtctg tagtcttacc aatgccgcct tttctacctg taagcattac
     4141 aacttttcct agtttatctg atgttttaaa tgcagtccgt aagttttcca tagttccctc
     4201 caatttccta tactaaatat atttagtata cttcatatac ttaatatatt cgatatatct
     4261 aatatactcc aaattgataa gaattcagac tttataagga atatagtgga tatactaaat
     4321 atatttgcta tattcagtat atcagatata ttcaaaatgt caaaatatat ttgtgcgttt
     4381 tgcgttgaca atttagagtg aaaagaggta aacttaattc tatagaaacc acagataatt
     4441 cactatttac aaagtagcaa aagcagtggt actctttaaa cagaaaaatt acataaaaaa
     4501 aagagacgga aataattgat tttagcagaa tcaattattt ctgaatcaca tggttaggac
     4561 atgtgttcac gctcgtctca agaatatgta agtagctacc aactacttat attaaaagtt
     4621 gatcggatac cttttccgat tgcctttatt ctaccaaaaa aaggcgagca actcaacaga
     4681 agaacgttta tttagtgtac ttaaattaaa tattcttaaa cactgaagag agaacacata
     4741 ctatggctga gattaaagct aaactgtgtt ctctttttgt ttgagaaagg acgttacaat
     4801 cgatgttgag cgaaatttat tcagctattc ttaaaaacgg cttacgtaca tacaaatatc
     4861 gaaacagcac catgaaacct gcaggtcata ttgattccag taggagtggt gctatctttg
     4921 cgtaccgtag caagcaatta atgaatgttg ggcgtggtat ggtcattact tcggaagaag
     4981 ctattttaga gaacgaaaag aaactaacgc attggacacc taatacctat cgctttggta
     5041 cttatgcgga tgattatcga acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa
     5101 tcaacacatt tgtagttgat atcgattcaa aagagaacca tcaaggcgaa attattttag
     5161 cctgtttaga tcaagtgggc tacatgccaa cgctaatttt agaatcagat catggttatc
     5221 aagtttattt tgtactaaag acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga
     5281 tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg
     5341 gagtggatct aggatgtaac cacttcggaa ttgctcgatt cccacaagaa aataatattg
     5401 tttttaaaga aatgaactat cagtattctt ttgctgactg gattcaatgg tcgatgaagc
     5461 ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc tttcctgaga
     5521 aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat gttacttcat aaagcaaaca
     5581 tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat tttcacgctt tctttagctt
     5641 attactcttc aggttatgca ttggatactt gcgaatacaa tatgttcaca ttcaacgaac
     5701 ggttacagga accgctatct gaagcagaat tagtaaagat tgtacgtagt gcttattctg
     5761 gtaattatca agcagcaagc agagaattca ttattgaatt gtgccagcaa tgggtttcac
     5821 caaccttaaa aaataatgat ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc
     5881 cacgagatca aagaaaatat agtcataaaa atgaatggaa agaagatcta ttggcttatc
     5941 taagcgaaaa aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa gaattggctt
     6001 cacaactaac gatgcccaag cgaacacttg ataagatctt aaatgagtta cgagaggaga
     6061 ataaagtctt ctacaaggta acacgaggaa gaaatggcgg aatgatcatt gcttcagtca
     6121 aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa gagactagat tattcaacaa
     6181 ggatcacaga ggtatttgga ctcaacgaga gcttagtaga agagacattt agacaattat
     6241 caaccccctt gaaccctctt aaagaagtga atctttttga agtagataca ggttgaaaaa
     6301 taaaaggtgc aaattgcctt atacaatata tctatgatac ttctgtaggt gttgctccct
     6361 ttgcctagct acgtgtaggt ggtatactta taactgcgta tgcagtctta tgtatcatgg
     6421 aggaagttgt atgtcgttaa aaaattatgt ctattcacat cctgttaatg tgtttatcat
     6481 tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt tatggtttta agcaggggac
     6541 tttgtcttct tgggttacca gggaaaaaac agttgcttct ttgccgattg agtttcttca
     6601 tgggttgtcc ttagcttctg gggagactat ggatcaggta tatgattgtt tgtgtgtgtt
     6661 agagcaggag tatattgagt ataaaatagc taacgagtta agaaaaagga agaagtatat
     6721 ccagtagtag tttttattta cttaaaaaat tggttcattg agcaacctat cgctgctaaa
     6781 gtagcaaagg aaaacgttaa aggaaagaat ttagggaaga aatcgaaatg aaatcggacg
     6841 actggattga acacacctgg cttcctctga aaagaaatag caaacgaaag aaattttggg
     6901 tattgcaatt atggccatta ggaaaacttt gtagttacgg ggcacgactt attcttgatt
     6961 taaccgttta ctcgattgga gtatttggaa cggcttatta cttgataaat gatcccaata
     7021 ggattgcagc atcagacatt tttacagtgc taatgacgtt gatttttgtc atgctattgt
     7081 ctatttttac tcaattagtc atgcaattta ccaggcaaaa attaacagca ggaaaacttt
     7141 tcaaaagcct attcgattta attgttgtaa atgcaattct agcgacttta ataaaacaaa
     7201 ttgaacaatc tggaagaaca acaaaaggag tgaaggattt tttattgacg tttattcact
     7261 ttgtaaatgg acacatgtat ttgtttgaag tgggtattat tctctttttt gtcgggtatg
     7321 tatccacgaa acttattaaa cgttcaattg aaaaagaaat tgaaattgac ctgattccga
     7381 cacttcttgg ttatgagtac aatgttgatt ttggaagata tgacgaattg atcaatacgg
     7441 atatcgttca cacgagtatc catgaatcta ctgttaaggg catcagatct attttaaaaa
     7501 atggccagga aatgaaagca atagatcgaa gaaaagagat ctacaatttt tatgaagtcg
     7561 aagcaagcct acgatatggc atcggaaaat tgtggttaga tggaagtaat attcgtcaaa
     7621 acaaaccata cattttttgg aacagtagtg atttagaact tccaatgaaa gataaataat
     7681 tatggagaat cgtttgaaaa tgattcctct atacaggatt gtgcttatag ttggcatgga
     7741 gttaaaaacc ttaaagaagg agcagttatt caaagtgaaa gaacgtcata atcagttact
     7801 atgtagtaaa acgggtacga tgaacattga ataccgaaca gaatatttag aattaacgaa
     7861 tcaaactgat tttttgttga ttcgaaagca acaagagcaa gaaaaaaatt aatggctact
     7921 tatagccaat tgattgatga atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc
     7981 taaaagcctt tatttgtaaa gagcataagg aaacgtggga acacttttta aaaaaagtgt
     8041 tccaataggc tgtgcctatt acgtttctca aaaataaatg tgggaacaaa atatttaaaa
     8101 ataggatagt gaaaggatag tgaaggatag taaaaggagg gtagtaggat agcaaaaagg
     8161 ataggctcct tttcgctatc ctttttaaat tggaggaatt aatatggaag taagaataag
     8221 aaatgtggat gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac ggttctgttg
     8281 caaagtttta aatctactat caaataaggt agaataatag aaaaagatag caggaggaat
     8341 gacgatgaat c
//
LOCUS       DACDOA010000110         7896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDOA010000110 DACDOA010000000
VERSION     DACDOA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.111"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..846
                     /locus_tag="IWI39_002372"
     CDS             19..846
                     /locus_tag="IWI39_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209429.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1119..1889)
                     /locus_tag="IWI39_002373"
     CDS             complement(1119..1889)
                     /locus_tag="IWI39_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9209430.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2107..3174
                     /gene="ulaG"
                     /locus_tag="IWI39_002374"
     CDS             2107..3174
                     /gene="ulaG"
                     /locus_tag="IWI39_002374"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9209431.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3192..3653
                     /locus_tag="IWI39_002375"
     CDS             3192..3653
                     /locus_tag="IWI39_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9209432.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3673..5157
                     /locus_tag="IWI39_002376"
     CDS             3673..5157
                     /locus_tag="IWI39_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9209433.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5200..5499
                     /locus_tag="IWI39_002377"
     CDS             5200..5499
                     /locus_tag="IWI39_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209434.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5514..6152
                     /locus_tag="IWI39_002378"
     CDS             5514..6152
                     /locus_tag="IWI39_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9209435.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6157..7017
                     /locus_tag="IWI39_002379"
     CDS             6157..7017
                     /locus_tag="IWI39_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9209436.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7007..7717
                     /locus_tag="IWI39_002380"
     CDS             7007..7717
                     /locus_tag="IWI39_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9209437.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         2583 a         1254 c         1694 g         2365 t
ORIGIN      
        1 aaagggaagg aaattgtttt gattaaagta gatggagcat tactaaaaga ataccgaaag
       61 aaaaaggggt taacacaagt acaattagca gaaggcattt gtgaacaagc aactgtcagc
      121 aatatcgaaa acaagaatat gtcgccaggc atcaatatct taagtgcgct ttgcaaaaag
      181 ctgaatgtcg agatctccga tatattagta gaaacagaag aagacaaaat caggaaaaaa
      241 ataaatgaga ttcaatcatt gaattctgta tcaaaatacc aagaatctta taaaatttta
      301 caaacgatcg atcaggaaaa agtaagtaat tctgatcttt taaatcaaat tttattttat
      361 caaggactgc ttgatcatct ttatgcgaaa aaaccagatg cagcattatt ttattttaat
      421 caagtgttgg aacaaacgac cgaaacagat atccatcact tacaagcagc tgcaaacgtg
      481 gcaatgatct acttgtcaaa aggagaatta gattttgcga aagtttatgt ggagcgtaca
      541 cttaaaatat tgagtgagac tgattttgat aatctgatgg tctgcattgt atattatgat
      601 atcgccacct attaccgtaa aatcgaggat tatgataaag caatccaatt atgtgagaag
      661 ggtattgagt ataataagaa acataaatcg acttatgcat tggaatactt gctttatgaa
      721 attgcttctt gccataagca attaggagaa gatgattatt tagaaagata tatggacgca
      781 aaaaaaatag cgcgtttcaa cggaaatgat tacgcggtaa aggtgattga aaacgacctg
      841 aaataaagtg gttgtgaaat cagtgttctg atttgaatac cgtttattcg aaaaaaggag
      901 gtgtgacttt tgtcatacct ccttttttcg atattgtttg attcctattt aggcaacata
      961 taacggttat aaaagaagca atagattatg atggtatcga ttggatggat acatcttggg
     1021 attcctttat caatttgagc gtagcgtgtt tttcatttat cgataaattt gttattcatt
     1081 tttttgataa tcctatttac atatttacgt cctggttctt aaaatgaatc tacttgaatg
     1141 gtttttatcc ccgtatttct gatctcctct aatacttttt cagaacaaaa tttatcagtg
     1201 atcaaataat caaagactga aatatcagca atcttgaact ttgatgtcag tccgattttt
     1261 cgatgatcag cgactaaaat cttacatttg attgtcttgt tgaccatcat ttcgttgatc
     1321 gttgattcat ttaatatctt tgttgtgacc ccatgtacta catcgactcc gctacagcca
     1381 atgatacaaa tgtctgcatt gactgaagaa atcgtattta atgcgatgtc gcccactagt
     1441 gcttcttttg gaaagcgcat ctctccacca gttaaaatat aactacaatt atctttgtgc
     1501 ggagagtaat tgattttcac gttgtttgta atgactgtca aatggttaga ttgaagaaaa
     1561 tccagtgttt tcaacgcagt cgagctagaa ttgataaaca gcgtatcata atcgttaatg
     1621 tatttactag cttctctggc aatcgcttgt ttaatccttt caggaccaga ttgatcaaaa
     1681 tcagtctggt ttttgtaatt ataagaacaa taaccgtaat tgcgtatgat ctcgtttttt
     1741 tcttctaata aatttaaatc tcttcgtatc gtactaatag atacatcaag ggattcagct
     1801 aattcgaaag ttgatagttg ttcagtctgc tgaagcagtt ccaaaatttc tttttgacgt
     1861 ttttcaatat tgacaagtga gtttttcatg acttttgcct cctgagggac atcttgatcc
     1921 atggatttat ttgactagtt gttcatttaa acattcatat ctgtctttat tatactgaaa
     1981 caatcattga ttactgaaag aaaatcatat aaaaaacaaa cggtcatata aaagggtttt
     2041 catggatgct gatacacatt aatatttact tgtaagttgg aacaacgata tggggggagc
     2101 aagcaaatga gtaaaactgt caatgaagta acaaaagaaa agtggttagt agaaacattt
     2161 cctgagtggg gcacatactt gaatgaagaa atcgaaacag aagtagtgga tcctggaact
     2221 tttgctatgt ggtggctggg ctgtacggga atttggctga aaagtcatga aggaacgaat
     2281 attttatgtg atctatggtg tgggacggga aaacgtagtc atgggaatgg catgatgaaa
     2341 aaaggccatc agatgatgcg tatgagtggc gttcaaaata tgcagcctaa tttacgtact
     2401 cagcctttcg taatcgatcc ttttgcgata aaaaatgtag acgctttaat cgttacacat
     2461 atccattccg atcatctaga tatccataca gccgcagcag ttcatcaaaa ttgtcctgaa
     2521 gcaacattca ttgggccaaa agaagtggtt cgcacgtggc aaaaatgggg aattcctgaa
     2581 gaaaaaacga ttacagtaaa gccaggtgat gcagtcaaaa tcaaagacat caatgtagta
     2641 gctttagaag cgtttgatcg gacagcgttg gtcacttgtg aagatccaga agaggtatta
     2701 aaaggaaaaa tgcctcagga gatggatcaa attgcagtaa actatttatt cgaaacatca
     2761 ggagggaatc tttatcatgc aggagattca cattactcca atttatttgc caaacatggt
     2821 aatgaacaca aaatcgatgt ctgtttaggg gcctatggtg aaaacccacg agggataaca
     2881 gacaaagtta cttctgttga tatgctgcgc atggcggagt cattgaatgc aaaagtagtg
     2941 attcctgtcc actacgatat ttggtctaat tttatggctg atccaaaaga gatcactgaa
     3001 atttggaaat tcaaaaagga tcgcctgaag tatggattta aaccttatat ctggcaagta
     3061 ggcgggaaat tcatctatcc gacaaacaaa gatgatcttg agtttaattt ttatcgagga
     3121 tttgaagatg tcttctcgat tgaaaatgat acaccgtttc cgtcgttttt ataagaaaga
     3181 aggggagttt cttgctgaaa tacttttttg accatcaatt gattcattac ccagataaag
     3241 aaatcacgga ttggcgtgaa gctatcgccg aaagctgtct tgtattgatc gataaggaaa
     3301 ttattgatga aacgtatgtg gaggagatca tccattgtgt agaaaaatac ggtccttata
     3361 ttgtgatcgc acctcaagta gcaatgccgc attcttcaga aaaaagtgat ggtgtttttg
     3421 gaacagctat ttcatttacc aagttgaaac agccaatccg ttttgaaagt gaaggggaga
     3481 atgaggagaa aacggctagt ttgtttttta cactcgcagc aaaagatcca gaagaacatc
     3541 tagaaaatat ccaaaaatta tccgaattat taatgacaga tggattggtt gagcgtctca
     3601 tctctacgaa ctccatcgat gattaccaag ttgtgatggc agaatataat taattgatag
     3661 aggagaaaaa agatggggga atttttgtta agcatttgga cgtattttgc aacaaatatc
     3721 ttgacgcagc cagcatacct gattggtttt atcgtgttat taggctatat tttgctgaaa
     3781 cgcccgcttc atgaatgcat cgctggattc ttaaaggcaa cggtcggtta ctttattttg
     3841 actgttggct ctggtggttt ggtcaataat tttcggccaa tcttggttgg actaaaggaa
     3901 cggttcaatt tggatgccat ggttacagat ccttattttg gacaaaatgc tgtggatgct
     3961 ggattaatgg agacatttgg ccgtaccttt ggagatgtga tgattttatt actgattgca
     4021 tttgttttaa atatcttatt agtccgtttt cagaaatata cgaaactgcg ttccgttttc
     4081 actaccggaa acgttcaggt tcaacaagca gctaccgctt tttggatttt actgttttgc
     4141 tttcctgatc taggtaggat acaagcattg atttttatgg gactgatttt aggttgttat
     4201 tggacagtag cttctaactt aacagtagga atcacgcagg aattgacaga aggtgccgga
     4261 tttgcagtgg cacatcagca aatgttcgga atctttatat ttgcaaaatt agctgagtgg
     4321 atgaaaaagc gggatgaaaa gaaaaataga tcaatcaagc aagacaaaaa aattgaagat
     4381 atcaaattac caggattctt gtcgattttc aatgagaata tggttgctac ttcgttactc
     4441 atgctatttt tctttgggat cattctgatt gtactaggca aagattattt gattcaagcg
     4501 caattcatgc aagaagggca gagtttctta ttctacatta tgacgacttc tttaaatttt
     4561 gcggtatatc tggcgatctt gcaattaggt gttcggacat tcgtcgatga actaactcaa
     4621 tctttccaag gaatctcaaa cacgatttta ccaggagctg ttccaggaat tgacgtagct
     4681 gctacttttg gctttggttc gccaaatgcg gtaacgattg ggttcttgtt tggtgctttg
     4741 ggacagtttt tgatgattgg tttactgatt ttattgaaat caccaacgat cgtcatagcc
     4801 ggtttcattc cgttgttctt tgataatgcc gttatagcag tttatgccaa taaccgaggt
     4861 ggattcaaag ccgcttgcct gttcccgttt ctttcaggaa tcatacaagt aggcggttca
     4921 gcattattcg ctacatggat tgggatgtca acatacggtg gttatatcgg gatgttcgac
     4981 tgggcaacag cttggccatt gtttacagta atcatgaaag tcctaggtta tgctggaatt
     5041 gccgtagttg tcattggttt attggctatt ccgcagttac aatatcggaa aaatcctgat
     5101 ggttattttt tgattgtaga agactatgac acatatgcag aaaagcagag agtataaaag
     5161 aaaatctgat tagacaacac tcataatgga gggattaaga tgagaatttt agtatcgtgt
     5221 gcaaatggat caggaacaag cttgatgatg atgagaagtg tggaaaaagc aatgaaagaa
     5281 ctaaacgtac caattaccaa aatccatcat tgtgccattt cagagggaaa aagttcagct
     5341 ggccaatacg atgtagtttt tacgccagtc aattttttga atatgtttac tcaggcagag
     5401 aaaaaaggag ttacagttat cggtatcaaa aatgtgatgt cagtcaaaga aatcagtgaa
     5461 aaatttgctg aaacaaagtg gtaccaaaaa aacaagtaaa ggaaggaact aagatgagca
     5521 ggccgaattt gcaaattgcg ttagatcaca atgacttaga acatgcatta ggagatgtta
     5581 tgaaagttgg tgatgtagta gatattattg aagtgggcac aattctttgc ttacaggaag
     5641 gacaaaaagc gattcgctgt attcgcagta tgttcccaga taaaaaacta gtcgctgata
     5701 cgaaatgtgc agacgctgga ggaacagtgg cgagtaatgt tgcaaaagca ggagcagact
     5761 ggatgacagt catttgttgt gcaacgattc caactatgga ggcagcacaa aaagaaatag
     5821 gtgaattgca agtagaactc tacggcaatt ggacttttga acaagccatg gattggcata
     5881 acatcggtat ccggcaggta atctaccatc aaagccgtga tgcgctgtta tctggtgaaa
     5941 cttggggtga aaaagattta tcgaagatca aaaaactgat tgaactaggc tttaatgttt
     6001 ctgtaactgg tggattaaat ccacacacat tacacttatt tgaagggatc gatgtgtata
     6061 cctttatcac tggtcgtggt attacagcag caaatgatcc gatgaaggcc gcacaaaact
     6121 tcaaagatga gatcattcgt atctgggggt aaggaaatgg ctcaaattgg gatttatgaa
     6181 aaagcgttac ccaaagatat ttcatggaaa gaacgttttt cccttgtaaa agaaatggac
     6241 ttcgatttta tcgagatgtc gattgatgag acggatgagc ggttagcacg attagattgg
     6301 tctgaagaaa agatagctga gctgagagaa gagatgttca gctccggtgt acgtatccat
     6361 tcgatttgtt taagtgcgca tcggcgattt ccgtttggtt ctgctgatcc agaaaagaaa
     6421 aaagcagcga aacaactaat gaaaaaagca atccatttgg cgcataatct aagcgtcaaa
     6481 gtaatccaaa tagctggata cgatgtttat tatgaagaga aatcaatgaa ttccagagaa
     6541 gactttatcg aaggaataaa agaaagcgta aaggaagctt ctaaatatgg catcattctt
     6601 tccgtggaaa tcatggatga tccatttatg aactccattt caaaattttt agaaatcaaa
     6661 gaacagattc attcaccttt tctacaagta tatccagacc taggcaattt gtccgcatgg
     6721 ccggaaaata atccggctcg agaacttgag aaagggatcg attatatcac tgctatccac
     6781 ttaaaagata cttaccctgt aacaaaagaa tattcaggcc agtttcgtga tgttcctttt
     6841 ggtagcggtt gtgttgattt tcttggatta ttaagacatt tgaaacgtct agattatgat
     6901 ggaacatttc tgattgaaat gtggagtgaa aaaagccaag actttcgaag agaaatccag
     6961 caggcaaaag actacttaag ttctaaattg aaggaggctg gctatgatgt cttgtgaaga
     7021 aaaaattgca gcaatgaagc aacgtgtcct tgaagcaaat ctggcattgc cgcgatacgg
     7081 attagtcaaa ttgacttggg gcaatgtgag tgaagtggac cgtgagctag gagtcatcgt
     7141 cattaagcca agcggtgtca gatatgaatg catgcaagca gatcaaatgg tggtaacaga
     7201 tttgtcggga aatataatag aagaagatag cttaaaacca tcttcagatc tccccacaca
     7261 tgttgttttg tatcaaactt ttgaagatat aactgctatt actcatactc attccacgca
     7321 ctccgtcatg tgggcgcaag caggacgcga tttgccagct tatggcacga ctcatgctga
     7381 tgctttttat ggtaaggttc cttgtacacg ccaattaacg aaagaagaag taagagaagc
     7441 ttacgaagtt catacaggga atgtgattgt cgagacgttt aaggaaagaa aattagatcc
     7501 aaacgaagtc cctggagtac ttgtctatgg acatggaccg ttcacatggg gggattcacc
     7561 tatgaaagca gtagaaaaca gtttgatctt agatgaaatt tgtctgatgg ccaaagaaaa
     7621 cgagctgatc aaccccaata tatgtgaaat cccgcaatat ttactagata aacattattt
     7681 tcgcaaacat ggaaagtatg catactacgg gcaatagaga tgctagggta agggtagaaa
     7741 attaaaagat aacacttgat agagagaaac agaggttgtg aagaaaattt atcacaacct
     7801 ttttatttat caaaaagtat gcattctaaa aggaaagagg aattttaagg aatcgagaga
     7861 atctttacag gagagttata tgtgatagat taaata
//
LOCUS       DACDOA010000111         7715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDOA010000111 DACDOA010000000
VERSION     DACDOA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.112"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            215..1108
                     /locus_tag="IWI39_002381"
     CDS             215..1108
                     /locus_tag="IWI39_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9209438.1"
                     /translation="MGKISLGIDIGGTFIKYALIDKQHRIKEKWKVETVKYNTADEFY
                     DYICSNIRNVDEIDKIGVSAPGLIDEDSNVKSYAAPNVAIMYGTNINNEINKRTNKKV
                     SAINDAKAAGLCEFKIGNAKGYKSSAFLIIGTGTGGCICDENGVVYGKDSFAGEFHNM
                     PFVNAEIGGLDKMGDYASITGLVNLYNKKANREEQVQYGNEVCKKYLNGNESAVSSVD
                     EWIGNIVAQLIVITVFYNPEVICIGGGISEENWFINKVRETYKKTCREYLEADFITTE
                     INRCKHNNDANILGAVIKASI"
     gene            1182..2621
                     /locus_tag="IWI39_002382"
     CDS             1182..2621
                     /locus_tag="IWI39_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9209439.1"
                     /translation="MIYEELDKFQDDFLWGSASAAYQVEGAWDKDGKGESVWDVYTRQ
                     PGTTFKNTNGNIAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPNGRNEVNEEGLK
                     FYENLIDELIANNIQPIITLYHWDLPQHLQDLYGGWESREIIHDFNEYCVTLFKRFGN
                     KVKHWVSLNEQNIFVTLGYVTAMHPPAVKDQKRMLEANHIANLANATVIESFKKYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGI
                     APTIEDGDLELLERVKPDFIGINYYQTSTVAFNPLDGVGQSEGINNTGKKGTTKESGL
                     PGVYKNAKNAHLETTNWDWAIDPTGLRIGLRRLTSKYNLPILITENGLGEFDKLEEDD
                     TVNDAHRIDYLSAHIKACKEAITDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYVDRD
                     ENDQKDLRRIKKNSFYWYKNVIRTNGEEL"
     gene            2669..2971
                     /locus_tag="IWI39_002383"
     CDS             2669..2971
                     /locus_tag="IWI39_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209440.1"
                     /translation="MKKILLVCSAGMSTSLLVTKMRNSAKEKGIEVEIDALPVAEAST
                     AVDNVDIVLLGPQVRFQKGTVEKLVKGRIPVEVMDMRLYGMMNGKAILEDALQKIN"
     gene            2990..4354
                     /locus_tag="IWI39_002384"
     CDS             2990..4354
                     /locus_tag="IWI39_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209441.1"
                     /translation="MSFMDKFQSAIERLLVPLSTKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMEGDDLLTGLTAISVYFIIYPDYFDNEGVNYLTNQYMGAQGLFVAILVGLLV
                     GELMSRLAKSDKLEIKMPEQVPPAVARSFKVLIPIIITTVFFSVLNYFVKMAAPGGLH
                     ELIYNIIQTPLTRMSQSLFSVIILALLSQLLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LHYAESGTTWGAPFPITYSGLATAFAEYGGSGATLGLIIAILIFSKNKESKSIGKLSF
                     VPGLFNINEMVIFGLPIILNPIYIIPFIIAPLVNIMIGYTAVMILKIMPPVTIGIPWT
                     TPGPLMPFLGTGGNIVALFVGFICLAVSVLIYSPFVIAANKAAIIEDEMENEIQSQEV
                     VESI"
     gene            4410..4724
                     /locus_tag="IWI39_002385"
     CDS             4410..4724
                     /locus_tag="IWI39_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9209442.1"
                     /translation="MEGIELTAFSIISNVGMAKSLAIEALRDARKGKYNEAEKKMAEA
                     SEYFVKGHHAHTSLIQKEAAGEKVELSLIIMHAEDQLMSTETTKLLIEEMIEMFKQLN
                     NK"
     gene            5058..6104
                     /locus_tag="IWI39_002386"
     CDS             5058..6104
                     /locus_tag="IWI39_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9209443.1"
                     /translation="MMNSTEIAKIAGVSRSTVSRVINNYSNVSEETREKVLRVIKEHE
                     YVPHASARMLAGSQNKVIGLFIIDLIHEEVGLKNRITKSPYYLEFTSSVIETASEMDY
                     LVLVHIIHSVAGYEKIKESFYNKTISGGIFIGQNDDDESIKTIIDRGYKVVLLDQSIR
                     PEDATYNQCMIVNADNYNGAYCATKYLIDKKHKQIAHITGGTVKFSSKDRIRGYKKAL
                     EDAGIEVNKNLIVNSEFVENAGYEAAKKLFNGNSEFSAIFASNDKIAFGAIKAIQERG
                     LKVPDDISVIGFDDIESSKYFNPPLTSMKMKLGEMADIATKSIVASIEGETKPSINVV
                     PVSLVERESCGILQ"
     gene            6086..6322
                     /locus_tag="IWI39_002387"
     CDS             6086..6322
                     /locus_tag="IWI39_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209444.1"
                     /translation="MWDFAVAKLAELVGADYLIILIEVDNVCVYVDLKSKVQRPKMLC
                     TLNLSKNRNISQTSPSLSNYTKIMQTFFFHDKII"
     gene            6716..7213
                     /locus_tag="IWI39_002388"
     CDS             6716..7213
                     /locus_tag="IWI39_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209445.1"
                     /translation="MKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLI
                     VDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIKKNGDIKVNIFPI
                     PFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLK
                     SYFRL"
     gene            7256..7417
                     /locus_tag="IWI39_002389"
                     /pseudo
     CDS             7256..7417
                     /locus_tag="IWI39_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(7597..>7715)
                     /locus_tag="IWI39_002390"
     CDS             complement(7597..>7715)
                     /locus_tag="IWI39_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9209446.1"
                     /translation="GFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         2687 a         1194 c         1628 g         2206 t
ORIGIN      
        1 ataaaaaaaa taaaaaaagt attgaaatgt aattgctttc agtatatgct atatctataa
       61 acgcgcgttc atgggatgtt catcgtataa aaaaaggtgg aaatgagcgt atcgtttttc
      121 tttttaacaa atcatgaacg cgcgtttatg agtttgaatc aagctgcaat ataagaaggc
      181 aaactgcaag accttcattt taaggagaag tgttatgggg aaaataagtt taggaataga
      241 cataggaggg acgttcatta agtatgcatt aatagacaag caacatcgca tcaaagagaa
      301 gtggaaagtc gaaacggtta agtacaatac agccgacgaa ttttacgatt atatatgttc
      361 aaatataaga aatgtagatg aaattgacaa aatcggtgta agcgcacccg gccttattga
      421 tgaagattcg aatgttaaat cctatgctgc tcctaacgta gctataatgt acggcacaaa
      481 tatcaataat gagatcaaca aaaggacaaa taaaaaagtg tccgcaatca atgatgcaaa
      541 agcggcaggt ttatgtgagt ttaaaattgg aaatgcaaag ggatacaaat cttcagcgtt
      601 tttgattata ggtacaggaa caggcggatg catatgcgac gaaaacggag ttgtgtatgg
      661 aaaagattcg tttgcggggg aattccataa tatgcctttt gtaaacgctg agataggcgg
      721 ccttgataag atgggtgatt atgcttcgat aaccggattg gttaatttat acaataaaaa
      781 agctaatcgg gaggagcagg ttcagtatgg aaatgaggtt tgcaagaagt acctaaacgg
      841 aaatgaatca gctgtgtctt cagtagatga atggatagga aacatagtgg ctcaattaat
      901 agtaatcacc gttttttata atcctgaagt gatatgcatc ggtggaggaa tatccgaaga
      961 aaactggttc atcaataaag taagagaaac gtataagaaa acctgtaggg agtatttaga
     1021 agcggatttt attactaccg aaatcaatag atgcaagcat aataacgatg ccaacatact
     1081 gggtgctgtt ataaaagctt ctatctaaaa tacacaacag ttttgtttca agaaagaatg
     1141 tttgaaaata aatttatatt gaaaacgaag gagaagattc agtgatatac gaagaattag
     1201 acaaattcca agatgatttc ctatggggat cggcatctgc agcgtatcag gttgaaggag
     1261 catgggacaa agatggaaag ggggaaagtg tatgggatgt gtacacccgc caaccgggga
     1321 caacatttaa gaatacaaac gggaatatcg cggtcgacca ttacaataga tacaaagaag
     1381 acgtagcttt aatggctgaa atgggattga aagcttatag attctcaatt gcatggacac
     1441 gtatttatcc caatggaaga aatgaagtaa atgaagaagg cttaaaattt tatgaaaact
     1501 tgatagatga acttatcgcg aacaatatcc aaccaatcat tacgttgtac cattgggatt
     1561 tacctcaaca tcttcaagat ttgtacggcg gatgggaatc aagagaaatc attcatgatt
     1621 ttaatgaata ctgtgtaacg ttattcaaga gatttgggaa taaagttaag cactgggtat
     1681 cattgaatga acaaaatata tttgtaacac taggttacgt aaccgctatg cacccgccag
     1741 ctgttaaaga tcaaaagaga atgcttgagg cgaatcatat agcgaactta gctaatgcta
     1801 ccgtaattga atccttcaaa aaatatgttc cagacggtat gattggacca agctttgcat
     1861 ttggaccagt ttatccattc agttgcgatc caaaagatgt tttatccgca gaaaatgcag
     1921 aggatttaaa ttgcaattgg tggctcgatg tttattgcaa aggtaaatat ccagtgttcg
     1981 cttttaaata ttacgaaaga cttgggatcg cacccactat tgaagatgga gatcttgaat
     2041 tgttggaaag agtaaaacca gattttatcg gaataaatta ttaccaaaca agcacagttg
     2101 cattcaatcc gcttgatgga gtagggcaat cggaaggtat caataacaca ggtaaaaaag
     2161 gcaccactaa agaaagcgga ttacccggag tttataaaaa tgcaaaaaat gcacatctgg
     2221 aaacaacaaa ttgggactgg gcaattgatc ctactggttt aagaattgga ctaagaagat
     2281 tgaccagcaa atataatctt ccaatactta ttacagaaaa cgggcttgga gaattcgaca
     2341 agcttgaaga agacgacact gtcaatgatg ctcacagaat cgattacttg agcgcacata
     2401 tcaaggcttg taaagaagca ataacagatg gtgttgatct tctaggctac tgcacgtggt
     2461 catttacaga tttgttaagc tggttgaatg gttatcaaaa acgctatgga tttgtgtatg
     2521 tggacagaga tgaaaatgat caaaaagatt tgagaagaat caaaaagaac agcttctatt
     2581 ggtacaaaaa tgttattcgc accaatggag aagagttata agaatcgttc acaatcaatg
     2641 aatatgaaaa aacaaggaga gatagaccat gaaaaaaatt ttactagtat gttcagcagg
     2701 aatgtccaca agtttgcttg tgaccaaaat gagaaactcc gcaaaggaaa aaggaattga
     2761 agtagaaata gatgctcttc cagttgcaga ggcaagtacg gccgttgata atgtcgatat
     2821 cgttctttta ggaccacaag tgcgtttcca gaaagggacc gttgaaaaac ttgtaaaagg
     2881 cagaattccc gtagaagtga tggacatgcg gctgtatgga atgatgaatg gaaaagcgat
     2941 tttagaagat gctttacaaa aaataaacta ggaaaggagg ctaaaaaaaa tgagttttat
     3001 ggacaaattt caaagtgcta tagaaagatt gttagtgcct ttatctacaa agttgaacgc
     3061 tcaaagacac atatgcgcag taagagatgc ttttatttta tcattcccat taacgatggc
     3121 aggatcattg atggtgttat tgaataatgt tttcttgtca gcagatggat tcatgtttaa
     3181 aatcttgcaa ttcgataaac tttttccggg tattgttaaa ttccaagcag tgttcgctcc
     3241 agttgttaaa ggttcagctg atattttctc gattctgatt gtgtttttga tagccagaaa
     3301 ccttgcgaaa ctaatggaag gcgatgattt gttgacaggt ttgaccgcaa tctctgttta
     3361 tttcatcatc tatcctgatt atttcgataa tgaaggagta aattatctaa caaatcaata
     3421 tatgggcgca caagggttgt ttgtagcaat tttggttggg ctcttggtcg gcgaactaat
     3481 gtctagatta gcaaaatcag ataagttaga aattaaaatg ccagaacaag tacctccagc
     3541 agttgcaaga tccttcaaag tgttgatccc gattatcatc acgacggttt tcttctccgt
     3601 cctcaactac tttgtaaaaa tggctgcacc gggcggatta catgaattaa tttataacat
     3661 cattcaaaca ccattgacca gaatgagtca aagtttattc tcagtgatta ttttagcatt
     3721 attatctcaa ttgctatggg ctatgggaat acacggacca aatacgattg cagctattcg
     3781 agataccatg ttctcggaag ccggaaacgc aaacctctta cattatgctg aaagcggtac
     3841 tacatgggga gcgccttttc cgataacgta cagtggttta gctactgctt tcgctgaata
     3901 tggcggttca ggagcgacat tgggtctaat cattgctata ttgattttct cgaaaaataa
     3961 ggaatcgaaa agtattggca aactttcttt tgtacccggt ttgttcaata taaacgaaat
     4021 ggttatcttt ggtttaccga ttattctgaa tccgatttac attattccat ttattattgc
     4081 gccactggtt aacattatga ttggttatac cgcagttatg attcttaaga ttatgccgcc
     4141 ggtaacgatt ggtattcctt ggacaacacc aggaccattg atgcctttct tgggtacagg
     4201 cggaaatatc gtcgcactgt ttgtcggatt catctgtctt gcggtgagtg tattgattta
     4261 ctcgccgttt gtaatcgcag cgaataaagc agcgattatt gaagatgaaa tggagaatga
     4321 aatacagtca caagaagtag tagaatcgat ttaaaaaagc tgcagtacgc tgagaacaaa
     4381 ccgaaagatt caagatagga gatttacaca tggaaggaat tgaattaact gcttttagta
     4441 taatcagtaa tgttggaatg gccaaatcat tggctattga agcattaaga gatgccagaa
     4501 aaggaaagta caatgaagca gaaaagaaaa tggctgaagc atcggaatat tttgtgaaag
     4561 gccatcatgc acatacttct ttgatacaaa aagaagctgc tggcgaaaaa gttgaacttt
     4621 cgttaataat catgcacgca gaagatcaat tgatgtcgac ggagacaact aaattattaa
     4681 tcgaagaaat gatagaaatg ttcaaacagt taaacaataa ataagtgaac atgaaagaga
     4741 aaatattaga cgaactagaa ttgcttgctt actttacagg cagaatctga tgccagcagc
     4801 aaaacataga gcacccagga tgaaatataa tcctgggtgc cttattttta gaacaaaaca
     4861 ccggggtggt agtttgttct gcggttgaag gcatttgctg atggtttctg catgaaaggt
     4921 attgccaaaa gtctcctttt gctggaatcc tgaagcaggc agagtttttc gaaaaacaga
     4981 aaaagattga aaaaaatgga caaataatat agtgttgcag tatgaacacg agtttacgaa
     5041 cgaaaggaga tgcctttatg atgaatagta ctgaaattgc aaaaattgcc ggagtatcac
     5101 gcagtactgt atccagagtg ataaataatt attcaaatgt ctcggaagaa acaagagaaa
     5161 aggttttaag agtaattaag gaacatgagt atgtgccaca tgcttcagca aggatgcttg
     5221 caggaagcca aaataaggtg atcggcttat ttattataga cctgatacat gaagaagttg
     5281 gactaaaaaa tagaattacc aaaagtccgt attatctaga atttactagt tcagtgatag
     5341 aaacagcaag tgagatggat tacctggttc ttgtccatat tatacatagc gtcgcaggct
     5401 atgaaaaaat taaagagagt ttttataata agacaatttc tggtgggatt ttcataggcc
     5461 agaatgacga cgatgaatcg ataaaaacaa tcattgatag agggtataaa gttgtgctgc
     5521 tcgatcaatc aatcagacct gaagatgcca cctataacca gtgcatgata gtgaatgcgg
     5581 acaattacaa cggcgcttat tgtgcgacaa aatatctcat agataaaaaa cataaacaaa
     5641 tcgcacatat tacaggcggt acggtcaaat tctcttctaa agacaggata agaggataca
     5701 aaaaagcatt agaagatgca gggatagaag taaataagaa cttgattgta aatagcgaat
     5761 ttgtggaaaa tgctggatat gaggctgcga aaaaattatt taatggaaat agtgagtttt
     5821 cggctatttt tgccagtaat gataagatag catttggagc aatcaaagct attcaagaaa
     5881 gaggtctcaa agttccagat gacatctctg ttataggatt tgatgatatt gaatcgtcta
     5941 aatattttaa tccgccattg acttctatga aaatgaagtt aggagaaatg gctgatatcg
     6001 ccacaaagtc gattgttgca tcgattgaag gtgaaacgaa gccatctata aatgtagtac
     6061 ctgtctcatt agtggaaagg gagagttgtg ggattttgca gtagcaaaac ttgctgaatt
     6121 agttggagct gattatttga ttattttgat tgaggtagac aatgtctgtg tctatgtcga
     6181 cttgaaaagt aaagtacagc gcccaaaaat gctctgtact ttaaatctca gcaaaaacag
     6241 gaatatttcc caaacatctc cttctttgag taattataca aaaattatgc agactttctt
     6301 ttttcatgat aagataattt aaaaagtttt taaacgattt ctcctcctct ttttcttata
     6361 tcgggggagc atattttttt gtagactagg ggattataag ttcagccatc aatgtctagc
     6421 tcccaagtcc tgacctagtg aaaaaaagat aaatttgccc cattgcgcgc ttcgctgctc
     6481 attcagggtc aaatttccta ttttttcata ggtcaaggcg gacttgtccg cttttcttat
     6541 attaagttta caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact
     6601 tttggttggg gtataaagtt atttcttttg aaactctttg aacaagggat tgcaaaaatt
     6661 gaagcaactt ttgtcgaagc tcaaaaaaat atgggctgtt ttacctgaac attctttgaa
     6721 gcaacgcatt ggtaataatc tagctttgat ttatagtgga gctctcctaa ttcaagatat
     6781 actgggctat cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca
     6841 gttaatcgta gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt
     6901 gttggtgatg aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac
     6961 gatttgggga aaagtatcaa ttaaaaaaaa tggggatatt aaggtaaata tttttcctat
     7021 cccgtttgag gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag
     7081 acaattgctt gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca
     7141 catgaatcgt caagttcttc caacatacga aattctatta ccttcttcac tcaaatcgta
     7201 ttttcgattg taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttattaagca
     7261 actgaaagat attcctattc caatgcccgc tatttcagaa caacaaaaaa tcggcacttt
     7321 cttcaaacaa ctcgacgaca ccattaccct tcaccaacgt aagttagatt tactaaaaga
     7381 aacgaaaaaa ggcttcttac aaaagatgtt tgtttaggag ggaagaaaaa tgaaaattag
     7441 tcaccgtgag gaagcggaag tcgaagaatg atggtgacac tcacaatgta ataaaaggtt
     7501 ttatgcccct tgatctatca actgggagaa aaattcgatt ttcagaaatt cagtatgaag
     7561 gaattggttt tactcttata gatattattt gtcaaattaa gcgagacaaa actttccatt
     7621 tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt agtgattttc tagggatatg
     7681 gtttcttttg gacgcaacgg atgagatgaa tcctt
//
LOCUS       DACDOA010000112         7629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDOA010000112 DACDOA010000000
VERSION     DACDOA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.113"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2002)
                     /locus_tag="IWI39_002391"
     CDS             complement(<1..2002)
                     /locus_tag="IWI39_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9209447.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWAT"
     gene            complement(2018..3014)
                     /locus_tag="IWI39_002392"
                     /pseudo
     CDS             complement(2018..3014)
                     /locus_tag="IWI39_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3212..4453
                     /gene="sstT"
                     /locus_tag="IWI39_002393"
     CDS             3212..4453
                     /gene="sstT"
                     /locus_tag="IWI39_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9209448.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4600..5235
                     /locus_tag="IWI39_002394"
     CDS             4600..5235
                     /locus_tag="IWI39_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9209449.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5426..6166
                     /gene="truA"
                     /locus_tag="IWI39_002395"
     CDS             5426..6166
                     /gene="truA"
                     /locus_tag="IWI39_002395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9209450.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6311..>7629)
                     /locus_tag="IWI39_002396"
     CDS             complement(6311..>7629)
                     /locus_tag="IWI39_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209451.1"
                     /translation="VVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAV
                     ALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYF
                     WDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHI
                     LNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHT
                     PVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNS
                     IHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHF
                     MSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINK
                     VVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2497 a         1198 c         1443 g         2491 t
ORIGIN      
        1 gtgtcgccca tgttgcactg tactggttac ctgcaacaag atcatattgt atcagacgat
       61 aatcttctag aatttctttt tgtttctcag ttaaattttt ttcttggata atttgactat
      121 cctctgtaac aaagacctgt tttccattaa aacgattaga caagctttct gtaggatcaa
      181 ttgttaatgc aggtagatga ttagatactt ctgtcaacaa cgcatagtaa ggagttactt
      241 tgatattcgc atgctcaaga gccatagcac caaagttatt aggagcaaca acttctttat
      301 ttaacgtagc tgcttgttca cgactatact tgttgttata aatgaaataa tctgtctggt
      361 gctgttctaa tccatatttt ttcgaatcat tcccactata gattgcgggt aaatggtcac
      421 cataaaaaac aaaggtaatc ggtttatcga ttttgtcgat ttcttggatg aattttttta
      481 ctgctgtatc ggtatagtga aggccttgca tataagtttg taattctttt cttctattag
      541 gaatgacagc agttccttca gcagtgtagt tcaattcact atagtaatca tcataaggca
      601 tatggttttg catagtggat aattggatga attgtgacgt ttctgtattg ctttgtaaag
      661 ctttcaaggt ttctttgtat gctgaatcat ccgatatgcg tgggctttgt tctattttat
      721 ccgtataagt taatttatcc ggactgtcta aataatagaa cctgtcaaaa ccaaatttat
      781 caaacacttg ttttctgcga taaagagcag ctgtaaaagg atggatggct attttttcat
      841 cgaatagatc ggtaatattt ggacttattt tctgacgctc cactagctgg gtataaggtg
      901 taactaacga agcagacagg ttactgatac tcaagcctgt cagtccttcc cattccatgt
      961 tagctgttcc gccaccataa ccgacggata acatcaatcc tcctgttcct tctgttataa
     1021 tgcttttgat gtaaggaata gggtctgtag gtactgtcag attagggaca cgtgcaggat
     1081 cactgaagct ttcacttaaa ttgagaacga tcgtctgatt gtttaaccag tcacttcttg
     1141 tttggttgat ttcatctgcc tttgtgttgt actttttcat tattccatta attttttctt
     1201 tcgaatagcc agcaggttct tccatgattt taatgtcgat attgtttagg aattgaatga
     1261 ctggtccgtt atcttttacg gcgccttgct ggttaaagaa agtcttgttt actttaaata
     1321 aattaaacag caagaaagaa ggcgaatttt tgtgattaat gaaaaagaca cccgaaaaac
     1381 atcctaataa tacagcgata ctgataaatc gacgtttcca atgaatcttc aaggaatact
     1441 gtttttgtag tctacgttgt acaataatac tagtaatcaa taataggaga aggatcaatc
     1501 cgcccacgat taataagact gggctaacca tcgataatag ttctgacaat ccggtcagta
     1561 tttttaaatc tgaaggaaag acaggctctt cccgcatttc cattttgatt actgtcgaaa
     1621 tagtgagcag taagttaatg atcaaggtag cagtcaaggc gtaccaaaaa cgattggaaa
     1681 tcaacaatag taagaagaaa aatcccataa taatgatgat tgtcaggatc atgggtgctt
     1741 gtcgctgagt aaatattttt aaggcaacct ggatagtttc tttccaacct tcttttggtt
     1801 ctaaaacgaa aatctgcacg aaggtaaagc agtaaaaaaa tgctgcaact aaaaaataac
     1861 ttttatgaac aaaaagccaa tgaattcttt ttttccaacg ctgagttaat tgttcaatcg
     1921 aatcaaaagc gaagtattcg atgaaacgat gaatgaaacg gattttttgt atataaaata
     1981 aaaagataac taggagggac actgctaata aatagcaaat aaaaaactgc ccgatattta
     2041 tcagccatgc cttccctgag tttggaaatt cacgtttcat aaatgtagat acgcgataag
     2101 aaactaaagg gaaattacat ataacttgag tgacaatcaa aaatacagca agaaaatttc
     2161 ctcttccttt tatacctaat ttatcagata gcatttctaa aaataaaaac gaagagaaag
     2221 tataatacat tgcgaagaga gaatatggtt tagaaaacct cagcgctgta tctgtatttc
     2281 ctctgatgat gagtgaaatt tgtgtcatca aaataatcaa accgaataaa acaatcaagc
     2341 tgataaatac ttgaggaagc ttatagcgga ccttttcttt ccagttccat tctttcaaac
     2401 cataacccat taaaaataaa taaaaaatcc ataaaatact ctgtccattc tgtaatgcaa
     2461 acgggtcttt attaaataga gtagagcagc caactaatcc aattgttaaa aataatagta
     2521 gatatttaat tgttgttttc gataatttat ttaaaaatcc aactgtataa ggaatactac
     2581 aaaaaagcaa agtacaagaa acagcataag taaagtaatt ctgactgata ggtgttagga
     2641 taagccaata atcacgaata ttcaagctgc cagcttgtac gtaaaaaaaa gatattgcaa
     2701 actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa aacttttaaa
     2761 cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc accaataaca
     2821 acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc atatgtagga
     2881 atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc gacaaagatt
     2941 gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct cacaaaaaaa
     3001 ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac atcctaactt
     3061 agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa gaaatggttg
     3121 tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata aaatgataca
     3181 atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg atatgtcgtt
     3241 agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct ttttccccac
     3301 atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg caatcgcacc
     3361 tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg ggaataagac
     3421 attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg cacttgtagc
     3481 agttattgta agttcaattt ttactgttcc tattacttta caagaaacag tatctgaaaa
     3541 agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca cacaaaatcc
     3601 tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg tattgttggg
     3661 actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga tatctatagc
     3721 gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt taggattggt
     3781 ttatcaatca attgctacga ctggaatagc tggattggca gagtatctgc agcttttact
     3841 tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc tcacgttttt
     3901 gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa gcgcacttcc
     3961 cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt tggctgaaag
     4021 attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta cgatcaatat
     4081 gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt ctttagggat
     4141 tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag ctgcttgtgg
     4201 agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta gtttgtttgg
     4261 tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg gtgttatcca
     4321 ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg caataggaga
     4381 gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt tagatggatt
     4441 aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt tttaatgagt
     4501 tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc gcttataata
     4561 acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt acgtaaattt
     4621 ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta cgtattagga
     4681 ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg agaggtttcc
     4741 agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt tttcatcctg
     4801 tcattgcctg cattgtatgc tgcatttgta agtacatctc gtgtgttagc taccctagtt
     4861 acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt gttcagtatc
     4921 aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg atcaggttta
     4981 ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg aaaaaatggg
     5041 caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct ttttgcggcg
     5101 atttatggac tggcaagaat tccttctata aattcttggc cattactgaa taaaataggt
     5161 ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgcctggtt gagtatcttg
     5221 catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac ttcatccggt
     5281 ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata gcttctgttt
     5341 tagagaagaa aggagctata ttttgtgcta caatcttaaa aaggtagaga gaagaccaaa
     5401 tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg aatatgacgg
     5461 aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc aaggaaaaat
     5521 cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat caggaagaac
     5581 agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca cagaaattgg
     5641 actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg tcatcaaaag
     5701 aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca aaaaatacag
     5761 ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca gcttttattt
     5821 ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag tgggtacaca
     5881 tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc gtacgattga
     5941 tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg gagacggatt
     6001 cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca caggtgccat
     6061 gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg caggagaaac
     6121 cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat actttgataa
     6181 tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat gaagcaaatt
     6241 aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa agtctttctt
     6301 aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt ttagaaaaag
     6361 taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt gaagcaattg
     6421 ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga atagttaatt
     6481 cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata gaagccactt
     6541 gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc gaattatcta
     6601 attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc tggttaccta
     6661 gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg tttgcataat
     6721 cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata agtgttaatg
     6781 aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt agtgcaagaa
     6841 acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa tagatcacta
     6901 tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag acaaattgta
     6961 gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt gaacaaaaaa
     7021 gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt tttataaaaa
     7081 taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg ttatagttat
     7141 tatttaaaat gtgtgtcagc atatcactag gagccatacc acttaattct tttcccagtg
     7201 ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat cccgcactta
     7261 aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt ttttggtccc
     7321 aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata gcgacagaaa
     7381 gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat ttttggtaat
     7441 ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt aaagcgacag
     7501 cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa aatgtataaa
     7561 taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata gaaaaataag
     7621 aaacgacca
//
LOCUS       DACDOA010000113         7479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDOA010000113 DACDOA010000000
VERSION     DACDOA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.114"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..1648
                     /locus_tag="IWI39_002397"
     CDS             257..1648
                     /locus_tag="IWI39_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9209452.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1721..2368)
                     /locus_tag="IWI39_002398"
     CDS             complement(1721..2368)
                     /locus_tag="IWI39_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9209453.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2376..3062)
                     /locus_tag="IWI39_002399"
     CDS             complement(2376..3062)
                     /locus_tag="IWI39_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209454.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(3068..4141)
                     /locus_tag="IWI39_002400"
     CDS             complement(3068..4141)
                     /locus_tag="IWI39_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9209455.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4363..5037
                     /locus_tag="IWI39_002401"
     CDS             4363..5037
                     /locus_tag="IWI39_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209456.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            5042..6382
                     /locus_tag="IWI39_002402"
     CDS             5042..6382
                     /locus_tag="IWI39_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9209457.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6531..>7479)
                     /locus_tag="IWI39_002403"
     CDS             complement(6531..>7479)
                     /locus_tag="IWI39_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9209458.1"
                     /translation="TREEALSVIERLRNEGYERDDIVIYTTDEAASRLGFDGLSGIDV
                     ETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRGDISDGKFVITVKG
                     YRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQNTTDPLNMTPGTD
                     PMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVTTGEVDIHKHVVND
                     TETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQVDVSKHTVIREEV
                     GIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2321 a         1381 c         1315 g         2462 t
ORIGIN      
        1 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
       61 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
      121 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
      181 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
      241 attaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
      301 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
      361 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
      421 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
      481 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
      541 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
      601 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
      661 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
      721 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
      781 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
      841 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
      901 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
      961 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
     1021 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
     1081 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
     1141 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
     1201 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
     1261 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
     1321 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
     1381 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
     1441 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
     1501 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
     1561 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
     1621 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
     1681 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
     1741 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
     1801 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
     1861 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
     1921 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
     1981 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
     2041 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
     2101 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
     2161 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
     2221 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
     2281 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
     2341 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
     2401 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
     2461 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
     2521 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
     2581 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
     2641 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
     2701 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
     2761 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
     2821 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
     2881 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
     2941 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
     3001 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
     3061 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
     3121 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
     3181 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
     3241 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
     3301 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
     3361 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
     3421 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
     3481 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
     3541 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
     3601 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
     3661 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
     3721 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
     3781 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
     3841 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
     3901 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
     3961 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
     4021 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
     4081 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
     4141 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
     4201 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
     4261 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
     4321 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
     4381 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
     4441 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
     4501 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
     4561 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
     4621 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
     4681 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
     4741 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
     4801 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
     4861 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
     4921 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
     4981 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
     5041 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
     5101 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
     5161 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
     5221 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
     5281 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
     5341 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
     5401 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
     5461 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
     5521 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
     5581 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
     5641 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
     5701 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
     5761 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
     5821 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
     5881 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
     5941 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
     6001 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
     6061 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
     6121 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
     6181 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
     6241 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
     6301 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
     6361 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
     6421 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
     6481 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
     6541 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
     6601 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
     6661 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
     6721 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
     6781 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
     6841 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
     6901 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
     6961 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
     7021 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
     7081 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
     7141 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
     7201 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
     7261 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
     7321 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
     7381 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
     7441 cgcaaccgtt caattactga taatgcttct tccctcgta
//
LOCUS       DACDOA010000114         7363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDOA010000114 DACDOA010000000
VERSION     DACDOA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.115"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(521..946)
                     /locus_tag="IWI39_002404"
     CDS             complement(521..946)
                     /locus_tag="IWI39_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209459.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1320..2291
                     /locus_tag="IWI39_002405"
     CDS             1320..2291
                     /locus_tag="IWI39_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9209460.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2323..3720
                     /locus_tag="IWI39_002406"
     CDS             2323..3720
                     /locus_tag="IWI39_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9209461.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3879..4502
                     /locus_tag="IWI39_002407"
     CDS             3879..4502
                     /locus_tag="IWI39_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209462.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5251..7344
                     /locus_tag="IWI39_002408"
     CDS             5251..7344
                     /locus_tag="IWI39_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9209463.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2503 a         1239 c         1490 g         2131 t
ORIGIN      
        1 ggttcattct gacccagatt tgattttgta caacccatca gtccttattg acaaagagcc
       61 aaaattacta catgatgtag aataagcctt tctatcgttt ggttatttta tattcaaata
      121 tttgaaataa ataaatcttt actctttgat ttgtaaactg aaatgatcca ctgtttgttt
      181 tactgatgaa gcttgatcaa aggaagaagc tgagtttgaa gttcataaaa atcagccata
      241 gtgagttttg cttggatttg tttatcttct tctacttttt acataatttt gaacaggata
      301 agattcaaat gttttaaatg aatctttgac tagtttgtga cagttgtcgc atatgctcaa
      361 gcttccttgg tcattaaatt ctgtatggat atttttgttg ttattacatg tacaataatc
      421 aggactatcg atcatccatt tcgtctcctt tacaatcatc ttgcttcttt actatagcag
      481 aaaagaaggg gagaaacaaa agaagatcca acagacggca ctatagactg tagatgttgc
      541 catcctcatc cttcaaacgg taattaccga tttttgtaaa tatgactgtt gtttcttttt
      601 tattttcttt tcctgaaata atatattcgt catctttggt agtgatcttg atatctgtca
      661 gcactgtttt ttcttcagat gattcagaag aatcagttcc ttgtgtctga tacgtgcgtg
      721 tccattccat ttttttatct gtaaatgtca actgtcccgc ggataaatcc atatctttac
      781 gtacttttag cgtatataca ctgttggtgg tatctgcttt ttccgctttt aagacagaag
      841 aatcggttgt ttcgtccgag gattggtcag agccgccaaa caaatcacag ccagtcaatg
      901 tcaaaagtcc cgcagacaaa ataaatagta cgattaattt cttcaaaata cttcccccat
      961 ttgataggat aaagaaatta taccattgga atgtaaaact tcaaacggat ggaagagaaa
     1021 aacgcacaaa acacccacaa atagagtaaa ttttagaata aaattttaaa aaggcaaaaa
     1081 atccgccact ttcagaaagc gttttaaaaa agtgttgcaa tcttttaaaa aaatgctata
     1141 ttaaaaatga accggttcaa atacttgttt aagtataatt aatagtgaga ggaagttgct
     1201 ctactgaaga ctttagttat atggatattg atgacaagca acactatgca aagaatgcgg
     1261 tcatcataat cgaacaacaa gaaagagaaa tcaaatgtaa ctggcaggag tgagaatgta
     1321 tggcatcgat ccgtgatgtg gctaagatgg ctggtgtttc agtgggaaca gtttcccgtt
     1381 acttgaatgg tcagcaactg aaggaaaaga acatgaaaaa aatagcagaa gcgatttcag
     1441 cgttggacta taaagaaaac atcattgcta aagggttgaa gaataatcga agcttctcta
     1501 ttggactttt gataaacggg atttccagcc gttttgggtc ggaagttgtc tcagggatcg
     1561 aaagagtagc tgaagaaaat gggtatagct tattattaag cgggtttgcc gatcagccag
     1621 aaaaaattga acaaaaaatt gaatacctga tgaagcatgt gatcgatggg ctgattgtct
     1681 tcttatccga agaagagtgg tctggatttg aaatgctcac aaaaatgagt attccagtta
     1741 tcgcattgaa ttgtccagaa ggaccagcag gtattgatac aatattagtg aatgatcgag
     1801 aaagtgtagc aaaagtgatc gctcatcatg gtgaacaagg acataaaaaa atcggattta
     1861 ttgctgccac tcaaacagac tacgtagcaa gagaacgttt agcaggagcc aaagaagcgg
     1921 tccaaaatga tccatcgatc caattagagg tctttacagg agactattct agaaaaagcg
     1981 gctattacgg ggcaaaagca ttattagaaa aaggggcaac tgctattttt gtcagcaatt
     2041 acaatatgtc gattggtgcc atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg
     2101 atattgcgtt cagtcactat gattatttag ataaaaacga tctgagtatc cttccgaaaa
     2161 tcacgatcac gccaccaaca gataaaatcg gggaacgagc agcagaacag ctgttaaaac
     2221 gaatcaaaaa agaagaggaa gaaaatgaaa aagtgattgt gatggaaaat atcatacatg
     2281 gaatcaacta aaacgaacta aaaaagaaaa agaggtgctt tgatgaaaaa atatcaattt
     2341 ccagcaaatt tttggtgggg ttctgcagca agtggcccac aaacagaagg aaggatttca
     2401 ggcgatggaa aaggagagaa catttttgat tattggtaca aaaaagaacc gcaaaagttc
     2461 tttgatcaag taggacctga aaaaacctcg caagtttata cgaagtatca agaggatgta
     2521 cagttgatga aacagactgg gcataattca tttcgaacat ctatccaatg gagccgtttg
     2581 atacctgatg aatctggaac aatcaacgaa gaagccgttc gtttctatca tcgttatttt
     2641 gatgagttga ttgaaaacgg tatcgaaccc tttgtgaatc tgtatcattt cgatatgccg
     2701 atgtatctgc aagaaaaagg cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa
     2761 tatgccaaaa gttgttttga attgtttggt tcgaaagtaa aaaaatggtt tactcataat
     2821 gaaccaatcg ttccagtcga agcaggttat ttatatcgat ggcattatcc agaagagtca
     2881 gatatgaaaa aagcgatcca agttggctat catgaagcgt tggcttcagc gttagccatc
     2941 aaatcttacc atgaaatgga gcaaggtggt gttattggta tcatcttgaa tctgacacca
     3001 agctatccaa gagacgaatc aaaccctgaa gatgttaaag cagcgcaaat agcagatgcg
     3061 ttcttcaatc gttcattcct tgatccagct gtaaaaggcg agtttccaag tgaattgatt
     3121 gagatagtaa aagaacttga tctcatgcct gtgatccatg cagaggattt gacggttatc
     3181 aaagaaaata cagtagatct tttaggtatc aattattacc agccacgccg ggtaaaagca
     3241 aaagaaacac caatcgatac taaaaacggt ccaatgcctg aggattattt cgacaattat
     3301 gaaatgcctg gtcgtaaaat gaatccttat cgcggatggg agatctatga acaaggaatc
     3361 tatgatattt tgatcaatgt acgagatcat tatggaaata tcgactgctt tatttctgaa
     3421 aacggtatgg gtgtagaaaa cgaagcacga tttatcaaag aagatggcat gatccacgat
     3481 gattatcgga tcgaatttgt ccaatctcat ctgaaatatg tccatcaagc cattcaagaa
     3541 ggtgccaatt gtttaggcta tcatatgtgg acatgtatgg ataactggtc gtggaccaat
     3601 gcatataaga atcgatatgg ctttatttca gtagacttag cacaagatgg aaaaagaaca
     3661 atcaaaaaat cgggtcattg gttcaaagac gtatcagata aaaacggatt tgaggcttaa
     3721 gaaagaatag ttgagagcaa atgcgtgaaa ttctccattc ttaccatttt gtgaaaaaga
     3781 agttcaatta tttacgatct ttttcaactt ttttgtcttt ctatttcatt ttttgtatat
     3841 aatagaatag tcaaaattaa ataggagtgg aggaaagcat gaggaaaaaa agaccgtttc
     3901 ttctcttttg gttattcggt tttttcttac tgcttatttt atccgcagca ttttcaggaa
     3961 aaatgacagc agatcttgct cagaaagaaa cagaaaaaat cgtagcaagc aaagaatcag
     4021 tagataattt caaaaaagaa ttctcaactt attggtgggc ggagtataaa ataactgcca
     4081 attacgaaaa aggcgaattg gacatttatt tgccaaaagg aaaagaagaa aatcaattta
     4141 ttaatatcga tgacgtactt agtgcgattt ctctcagaca atatcaattt aaagacaagc
     4201 atttgaccgt tttgcttttg actcatgatg gcgacaaagt agcccgtttg aaaaatggaa
     4261 aagtgatcac cgcttattct gaaaaagtag acaaaaaagt ggtcgccgat cgtttagcgg
     4321 aatggaaaaa gacaaatgaa caagtcaaga aaacaactgt ctcttctcaa aaaaacgagt
     4381 cgacaggaac aagtccttca gattctggca gtggacaaac gataacggga agtgaagaaa
     4441 atcaagcctc aattattacg aatgagtcat cacaaggaca atatcaatcc tatggacaat
     4501 gaaaaacaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggcaaa
     4561 atagttcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
     4621 gtgtatcaag aggtcttgag cctgccgttt aactctgagc cacaaggagc aatttcaaaa
     4681 aatagctcct tatatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
     4741 accgtttacc aagaggttgt aaaattagcg tcctgacata atctaatacc gagggtcagc
     4801 tgattgaaca ccgtgtatca agaggtcttg agcctgccgt ttaactctga gccacaagga
     4861 acaatttcaa aaaacagctt ctcatgtttt ataaatattg tgacttgtgg ccgaggagtt
     4921 ggcaggagaa caccgtttaa ggaagaagtt gtgaaaaagc tgtcctgcat caagaaataa
     4981 ggagctgcga caagaatttt gtcacagcct ctttttttgt acgaataccg agaaactggc
     5041 ttttgaatac cgtggagtga agcggtcacc acaagacttt tatctcttca ttagtttaat
     5101 cgcttacatc gcagcggttt tatgctgtga aaagtggacg gaataatctt tagaaagaat
     5161 aaaaaaatct taagataaca acttttttag gaaattttca tttttcagga gcaaaataag
     5221 aaaaaatgtg aatagagaga agtgatatac gtgacacaac ttaaaaaatt gtgccaaaat
     5281 cgtttagcaa tcgttttgac agctgttttc tttttctggc tgaagacgat cattgcctac
     5341 tatgcagatt tttcattagg tgtggaaggt acgatccagt actttatctt gtggatcaac
     5401 ccaattgcga ctaccttatt gttttttggc ctttctttgt atattaaaaa gccaaagcca
     5461 gcgttagcta ttcttttgat cattgatatt ttaaatacgc ttttgttgta cttgaatatt
     5521 attttttatc gggaattcac agatttcatt acagtcaaat ctgtattagg cttttcgaaa
     5581 gtgtcgcaag gattgtcagg aagctccttt tctttgatga agccccatga tgtcatctac
     5641 tggctggata tcgctgtgtt cattgggcta ctggtttggt tgaaggtcaa gaaaatacct
     5701 atcaaaagca accctgtcgg caaaccgatg gctatcgctg ttacctgtct ttcagggctt
     5761 attttttcag gaaatctagc gctgagcgaa gccaaccgtc cacaattgct gcagcgaaca
     5821 tttgaccgtt catatattgt caaatatcta ggaatcgatg cgtatactat ttatgacgga
     5881 atcaaaacag gaatgactag cagtgttcgc gcgcatgcaa gcagtaatgg gattgatgaa
     5941 gttttagatt atacaaagaa acattatgca gagccaaatc ccgaaacatt tgggatcgct
     6001 aaagggaaaa atgtgatcgt tctccattta gaaagtttcc agcagttttt gattaatatg
     6061 aaggtagacg gacaagaagt cacaccgttt ttaaatagta ttttccagaa tcaagcaacg
     6121 atcagctttg acaatttctt tcatgaagta ggacagggaa agacaagtga tgctgaaaat
     6181 atgttggaaa ctggcacatt tggtttacct caaggctcat tattcacaga acttgggtca
     6241 gacaatgtct ttcaagccgc accagcgatc cttggacaaa agcaaggcta tacaagcgct
     6301 gttttccatg ggaatgtagc tagtttttgg aatagagatc atgtatataa aaacttaggt
     6361 tatgacaatt tctttgatcg atcttatttt gatgaatccg acgaaacgtt aggttatggg
     6421 atattagata aagatttatt cagagaatct gcccaatatc ttgagcatct acaacagccg
     6481 ttttacacga aatttctttc cgtaaccaac cacacgcctt attatacgga tgacaagaat
     6541 ttcgactttc catctttgaa tactggcaat agcacagtag ataattatgt tcgaacggca
     6601 cattatctgg atcaatcatt ggaacaattt tttacgtatt tgaaaaaatc tggtatttac
     6661 caaaattcaa tctttgtgat ttacggggat cattttggga tctcgaatac agataataaa
     6721 gatttagcaa gcgctcttgg taaagatcca gatacatggg atgaatttga caacgcacag
     6781 atgcaacgag tgccattgat gatccatatg cctggttata ctaaagggac agtcaatcat
     6841 gaatacggtg gagaaatcga tgttttgccg acattactac atctattggg aatcgatgat
     6901 aaagattatc ttcattttgg tacagactta ttatcttctc agcatgatca agtagttgct
     6961 tttcgaaatg ggaactttgt gacacccaaa tacaccgtcc taggaggaaa agcttacaat
     7021 aatcagacag gagaaatcat cgatacgaaa gcagcaggaa tcgatgagga aatctccaaa
     7081 gatcaggaaa aagtcaagac tgcgttaagt ctttcagata agctgaatca ggaaaatctc
     7141 ttgcggtttt atgtgccaaa cggatttgag acgattgatc cgaaaaaata tgactataaa
     7201 aatgaagcgg aacgtaatga agcaatcgaa aagcaaaaag gcgaacattc gacaagtgtc
     7261 ttttcaaaaa atggtaataa aacaacaacc aacttatatc cagtaacaag aacagacgtt
     7321 gaacagcaaa aaaataatca ataaacaatc aaaccaacct tca
//
LOCUS       DACDOA010000115         7344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDOA010000115 DACDOA010000000
VERSION     DACDOA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.116"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            125..2734
                     /gene="clpB"
                     /locus_tag="IWI39_002409"
     CDS             125..2734
                     /gene="clpB"
                     /locus_tag="IWI39_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9209464.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2885..3556)
                     /locus_tag="IWI39_002410"
     CDS             complement(2885..3556)
                     /locus_tag="IWI39_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209465.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3549..4043)
                     /locus_tag="IWI39_002411"
     CDS             complement(3549..4043)
                     /locus_tag="IWI39_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9209466.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4063..5283)
                     /locus_tag="IWI39_002412"
     CDS             complement(4063..5283)
                     /locus_tag="IWI39_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209467.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5430..7265
                     /gene="lepA"
                     /locus_tag="IWI39_002413"
     CDS             5430..7265
                     /gene="lepA"
                     /locus_tag="IWI39_002413"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9209468.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2420 a         1401 c         1507 g         2016 t
ORIGIN      
        1 gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac caaaattgac caaagcgata
       61 taatagtttt tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac
      121 ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca
      181 gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt
      241 acaatctaat cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt
      301 tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca
      361 atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg
      421 tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt
      481 aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg
      541 taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca
      601 atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat
      661 ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa
      721 aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga
      781 agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat
      841 tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga
      901 aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat
      961 cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa
     1021 tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga
     1081 tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt
     1141 agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt
     1201 tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc
     1261 tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc
     1321 cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag
     1381 acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag
     1441 taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc
     1501 gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc
     1561 tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg
     1621 cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga
     1681 agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat
     1741 tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg
     1801 agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc
     1861 agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg
     1921 tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa
     1981 agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga
     2041 atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta
     2101 cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt
     2161 ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga
     2221 cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat
     2281 gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc
     2341 agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt
     2401 gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat
     2461 cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac
     2521 aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc
     2581 aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt
     2641 tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca
     2701 tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa
     2761 aaggggtctg acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag
     2821 tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct
     2881 tttattaggt atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt
     2941 tactttgaga ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt
     3001 cgcccatcgt atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa
     3061 aacgttgaag attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca
     3121 cgccgcaaag tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg
     3181 atatctcagc atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca
     3241 ttggactcac cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc
     3301 ctgtgcctaa aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg
     3361 gtattttaag tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta
     3421 ataaagattc cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt
     3481 cataaaaatt atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt
     3541 tgatttcttt aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac
     3601 tcaatttctg aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc
     3661 aggtagtgaa attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt
     3721 ccgccttgct gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc
     3781 acaccgattt ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt
     3841 aaatcactag ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa
     3901 gcagttttgt cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag
     3961 agctctctga tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt
     4021 ggtgcggcaa aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa
     4081 gtaagcttcc atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc
     4141 taaccgttcg attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa
     4201 tgtttggata tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc
     4261 ctgttcatcc catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat
     4321 gatgaaatga aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac
     4381 atcaacgatc cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat
     4441 caaaaatgct aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa
     4501 atgtgcggaa ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc
     4561 ttttacgtga aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt
     4621 gctatcgtag tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc
     4681 ccaatgaaaa tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac
     4741 ggaaaatacg tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg
     4801 tttaggtagc tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc
     4861 tttacgccga tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt
     4921 gcgcatatta tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc
     4981 tggataagtt ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac
     5041 ttcacaagga tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac
     5101 tttcgatact tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc
     5161 gctttcctcg atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg
     5221 atcgatatgt tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc
     5281 cattttttca tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac
     5341 ttctatagct gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa
     5401 gtgctgatta tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag
     5461 agcagattcg taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg
     5521 atcgtatttt agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag
     5581 actcgatgga tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca
     5641 attacacagc gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg
     5701 tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg
     5761 tcgatgcagc acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca
     5821 atgatttgga gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac
     5881 gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca
     5941 gtgccaaatc agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg
     6001 caccaagtgg agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata
     6061 gttaccgcgg tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca
     6121 aaatcaaact gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc
     6181 caaaagcagt tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta
     6241 tcaaaactgt ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg
     6301 ctgaagcttt accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa
     6361 ttgatacttc tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg
     6421 ctgctttaca attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat
     6481 tcctcggact tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag
     6541 aattgatcac gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg
     6601 ctacagtaga taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac
     6661 cattcgtcaa agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt
     6721 ctcaacgaaa acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg
     6781 tcgtctataa tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa
     6841 gcacgaaagg ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag
     6901 tgaagatgga catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc
     6961 gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac
     7021 gccaacaatt tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag
     7081 acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta
     7141 aacgtaaact cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg
     7201 tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga
     7261 aatagcaagg gtttgtttga aaacagtgta aaaaataaac catgtttaca tggatttgcc
     7321 accggtttgc caccggtggt tttt
//
LOCUS       DACDOA010000116         7266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDOA010000116 DACDOA010000000
VERSION     DACDOA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.117"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            285..554
                     /gene="rpsN"
                     /locus_tag="IWI39_002414"
     CDS             285..554
                     /gene="rpsN"
                     /locus_tag="IWI39_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9209469.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            569..748
                     /gene="rpmF"
                     /locus_tag="IWI39_002415"
     CDS             569..748
                     /gene="rpmF"
                     /locus_tag="IWI39_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9209470.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            780..1157
                     /locus_tag="IWI39_002416"
     CDS             780..1157
                     /locus_tag="IWI39_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209471.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1178..1327
                     /gene="rpmG"
                     /locus_tag="IWI39_002417"
     CDS             1178..1327
                     /gene="rpmG"
                     /locus_tag="IWI39_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9209472.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1351..2334
                     /locus_tag="IWI39_002418"
     CDS             1351..2334
                     /locus_tag="IWI39_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9209473.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2364..2486
                     /locus_tag="IWI39_002419"
     CDS             2364..2486
                     /locus_tag="IWI39_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9209474.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2504..4027
                     /locus_tag="IWI39_002420"
     CDS             2504..4027
                     /locus_tag="IWI39_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9209475.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4051..4290
                     /locus_tag="IWI39_002421"
     CDS             4051..4290
                     /locus_tag="IWI39_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209476.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4483..5118)
                     /locus_tag="IWI39_002422"
     CDS             complement(4483..5118)
                     /locus_tag="IWI39_002422"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9209477.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5139..5357)
                     /locus_tag="IWI39_002423"
     CDS             complement(5139..5357)
                     /locus_tag="IWI39_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209478.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5666..6493
                     /locus_tag="IWI39_002424"
     CDS             5666..6493
                     /locus_tag="IWI39_002424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP9209479.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6682..7089
                     /locus_tag="IWI39_002425"
     CDS             6682..7089
                     /locus_tag="IWI39_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9209480.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2564 a         1082 c         1428 g         2192 t
ORIGIN      
        1 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
       61 gtgaattctt atttttcaga gcttaaactg catattcttt taaatgattt gattatctct
      121 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
      181 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
      241 cgatttgaga tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
      301 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtacgcagat aaacgtcagg
      361 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
      421 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
      481 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
      541 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
      601 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
      661 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
      721 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
      781 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
      841 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
      901 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
      961 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
     1021 aggagttatt tcaatgtatt gatggttatt gggagatacc tgttgatatg aaagaaaatt
     1081 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
     1141 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
     1201 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
     1261 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt tcaaagaagt
     1321 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
     1381 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
     1441 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
     1501 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
     1561 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
     1621 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
     1681 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
     1741 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
     1801 atagcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
     1861 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
     1921 gaaacgattc aaaaaaagga tttttataat ttggaaaaat tcaatcatcc cttagttttt
     1981 gaaagtgatt catcagaaaa tcactataat ggcaaacact atcatcaaca tcatcaagaa
     2041 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttattc
     2101 caattaattg accggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
     2161 attgctttgc aagatcaaga ttttatagtt gccatacaag gggtaaacga acaggttaat
     2221 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaattgg aaaaatgttg
     2281 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
     2341 gtattgatta ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
     2401 ttaaaagccc gaatatcaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
     2461 aaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
     2521 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
     2581 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
     2641 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
     2701 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
     2761 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
     2821 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     2881 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     2941 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     3001 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     3061 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     3121 gacacaacat gcagcctttg gatatttagc tttggattat ggattgatac aagttcctat
     3181 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     3241 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaatcgc
     3301 aaaaactttg gccaatgaag ctgggataaa attggaagta ttgaatcctc ttgaaagtct
     3361 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     3421 agcgttagag aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     3481 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     3541 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     3601 tgaagttttc gattacaaag caaaactcaa taaagacatg acagctgccg agtacaaaga
     3661 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     3721 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     3781 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     3841 tgctggtcgt tttaaatata ttcaatttag cgatcatgga attgcaccaa gtaaggcaga
     3901 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctttataatg aaatgcataa
     3961 ttggcctact ttctatccag cttcattatc tgagcatgag attgctcaag aaatgatggc
     4021 ccattaaaat ccaatctttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     4081 tccaagaata ttgatgctta tcggcgttat caatttgcaa agcaaatgag attaaatggg
     4141 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     4201 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     4261 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     4321 ttatttggat ttgatctgct ttttattatt tcttgaattg tcaaatcaag cgcaacactt
     4381 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     4441 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     4501 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     4561 aggatctgga aaataaaagg gcatattatt caatacttct gtaactgcga aatgattgga
     4621 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     4681 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     4741 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac tagttttgcc
     4801 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggatg
     4861 gccatggatc gaatgagtaa ggcaaatctt cccacgagac tcgacatgat tttttgtatg
     4921 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     4981 ttctgtatcg aaaagatcat tgcaaattgc acggtagatc gtgataaaac taattgaaaa
     5041 gtccatatgt tcatgcttca aacgattcga gattttctct ggcgaccact gaaaatcgac
     5101 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     5161 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgattagt ttttttcttg
     5221 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     5281 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgtttgag tatagtaaga
     5341 tgacaatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     5401 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     5461 gaaagaggtt ccctatgaaa aaatacctat tacaatctta actggctatc ttggttcagg
     5521 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
     5581 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
     5641 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
     5701 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
     5761 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
     5821 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
     5881 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
     5941 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
     6001 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
     6061 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
     6121 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
     6181 aatgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
     6241 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
     6301 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
     6361 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagccata aaagtattga
     6421 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
     6481 aaacccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa taaactagtt
     6541 tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg ttgtttaact
     6601 gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa aaattctaat
     6661 atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa gataggcgat
     6721 gtcataatta ttggcatttt gattattccc tcttttattc ccttatttgt attctctata
     6781 gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttca aattgatggc
     6841 aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa ttatcatcct
     6901 gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt taaagaagat
     6961 aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg tcaaacgagt
     7021 atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcaa agattactac
     7081 atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat tattattaat
     7141 aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag aagcggtgac
     7201 aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc ggttagatta
     7261 cttatt
//
LOCUS       DACDOA010000117         7227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDOA010000117 DACDOA010000000
VERSION     DACDOA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.118"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..206
                     /locus_tag="IWI39_002426"
     CDS             <1..206
                     /locus_tag="IWI39_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9209481.1"
                     /translation="KVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGY
                     RNQRLAQRILDNELGCANASEAV"
     gene            341..562
                     /locus_tag="IWI39_002427"
                     /pseudo
     CDS             341..562
                     /locus_tag="IWI39_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            562..909
                     /locus_tag="IWI39_002428"
     CDS             562..909
                     /locus_tag="IWI39_002428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209482.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            906..1955
                     /locus_tag="IWI39_002429"
     CDS             906..1955
                     /locus_tag="IWI39_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9209483.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1970..2176
                     /locus_tag="IWI39_002430"
     CDS             1970..2176
                     /locus_tag="IWI39_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209484.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            2197..2793
                     /locus_tag="IWI39_002431"
     CDS             2197..2793
                     /locus_tag="IWI39_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAP9209485.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3745..4344
                     /locus_tag="IWI39_002432"
     CDS             3745..4344
                     /locus_tag="IWI39_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209486.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4393..5109
                     /locus_tag="IWI39_002433"
     CDS             4393..5109
                     /locus_tag="IWI39_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAP9209487.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            5123..5902
                     /locus_tag="IWI39_002434"
     CDS             5123..5902
                     /locus_tag="IWI39_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9209488.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5985..6341
                     /locus_tag="IWI39_002435"
     CDS             5985..6341
                     /locus_tag="IWI39_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9209489.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            6331..6597
                     /locus_tag="IWI39_002436"
     CDS             6331..6597
                     /locus_tag="IWI39_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209490.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6756..7034
                     /locus_tag="IWI39_002437"
     CDS             6756..7034
                     /locus_tag="IWI39_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209491.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(7031..>7227)
                     /locus_tag="IWI39_002438"
     CDS             complement(7031..>7227)
                     /locus_tag="IWI39_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9209492.1"
                     /translation="NNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKE
                     GTLFGFSVCTEIRILLGIPA"
BASE COUNT         2633 a         1030 c         1410 g         2154 t
ORIGIN      
        1 tgaaagtaga atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt
       61 tatttgacta tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga
      121 caccggttgg ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat
      181 gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca
      241 tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct
      301 tataaaattt gtcaaaaaaa ctgttgccat tccacttaaa acaactgaat ttgatattct
      361 acacgctcta atgtccacac ctaataaagt cttttctcgt agtgatttga tagagaaagt
      421 taagggaata gaatttgatg gactagatag gagcatcgat tctcatataa aaaacttaag
      481 acataaaatt gagaatgatg ctaaatcacc tatgtatatt ttgacagtcc atggtacagg
      541 atacagattt ggtggccact aatgagaaaa agaacgataa aaactcaatt agctgtttcc
      601 tttttagcaa tcgcaacatt aataatagga tcgattagtt tagtcgcatt aagtttgatg
      661 aacaatcatt ttagtaaata cgtggaagag cggcaagaag atttgttgaa tcagtatgta
      721 tatacaattg atttattgtg gttaaatagt ggggaaacat ggaatagtga agaacttgca
      781 gcactatctg agaaagtatt agagaataat atttattttt ctatagaaga cgaacaaggc
      841 aacatggttt gggaacttac tggaaaagat ttgaagtcag cacaggagaa actgaaaaaa
      901 atgcattgaa agtttctgag aaaaacagtg tgaaactaga cgaaacaata gaagtgaaaa
      961 agaagttaat taatgatgga aacgagtttg gaaaagtaac gttttattat ttcggacctt
     1021 ttgcttatac agagcacgat gccttgttta tctccagtat gaaacaaagt ttaatgtatg
     1081 ttgcaattgc agctcttctt gtatcattca tacttgcttc gtggatctct gctagattag
     1141 gattgccact aaagcatgta agcgatttta cacataaatt aactcgtggg gaatatgctg
     1201 ataaaatccc acaagaaact tcaattattg aaataaattc cttaattgat tccctaaatg
     1261 atttaagtaa tcagttggag aaacagcatg ggcttcgaaa aagattaact accgatattt
     1321 cacatgaatt aagaactcca ttagctacgc ttaaaggcaa tgtggaagga atgattgacg
     1381 gagtctggaa aataactccc gagcgcctac aatcttgtta tgacgaaata gatcgtctaa
     1441 ctcgtttaat tggaaatata gaaataatca acaaaattga agcaaaatat gatcatttaa
     1501 ataaaactga gtttaacata tataagttga ttgaatccgt tattgaaaat ttcgccagca
     1561 aaattgagtc aaaaaacctt catgttgaaa tccaagggga taacatcaat atatctgcag
     1621 ataaagataa aatgaaccaa gttgttatca atcttttgaa taatgctatt aaatttacgc
     1681 aaaaggaggg aaccattaag ttttctataa gtaaaaataa agaccatgtt cttttaattg
     1741 ttgaagacaa cggcatcggt atagaaaaag atcagcaact tcatattttt gatagatttt
     1801 atatggctga cccctccaga agtagagctt taggggggca aggtatcgga ttagctattg
     1861 taaagagcgt tattgaagcg cataaaggta gtataactgt aaaaagtaaa ttaggtctag
     1921 gaactaaatt tgttataaag ttaccatttc aataaaggag gagagttatt tgtttcaaaa
     1981 acagataaaa attgctaaac tagtatgtga atgttgggca aaaaaaatat tttttcaact
     2041 gatttcgata gatagtgtcg aaaaagttca tttagatatc ttatcaaatt ctgtagtaat
     2101 cttttctaag aaggacttat caatagctac aattaaaaaa gaagtttttg gattacaaat
     2161 ttatgagatg acctaaatgt gattaattaa aggaggatga agacagtgaa aaagatgaca
     2221 aaattagtat ttgcaagtct tggaattatt gtatttttag gagcgtgtag caatcaaagt
     2281 gaatcgaata attctaatag cacaaacgag gaatcgactt ctacagcgtc tagtgaaatg
     2341 aatagtatgg agggaatgaa tcacgaaggt atggttcctt cttcaatgaa agatgctgcg
     2401 aaccctaaat tcccggtggg aagtaatgtt atactgctag gcgatcatat ggaagggatg
     2461 aaaggtgcaa atgctcaagt tgtaggggcc tttgatacta caatgtatga agtaagctat
     2521 gagccaaaaa ctgggggacc aatggttaac aatcatcgat gggtagtaca agaagagtta
     2581 aaagatactg agactgtagc aaatgagggc gatactgtta tcttgaatgc cgatcacatg
     2641 gatgggatga tgggagctga agcaaaagta gataaatcta ttacaggaac tgtttacgtt
     2701 gttaattaca ctccaaccga tggtcaagaa gaagttaaga atcatatgtg ggtaactgaa
     2761 gatgagatgg aatatgacga aaataatgag taattttata tactaaacca acaagctggt
     2821 acatggtctt cgtattctca acatgaggaa tatgagctct ttgttaataa ggactgatga
     2881 gttgtgcaaa atcaaatctg ggtcagaacg aacctcattc tggctcagat ttttggctct
     2941 tcgacattta gtgctggtag agagacaact gaaaatttag tcataaccag ttaggcagag
     3001 tgcttaactg gtttttgttc gcatttatta ttcttttgat attggctgct tgcccttcaa
     3061 ttttatttgg tttcttaatc aactcttcat ataaccccaa atcatatatt aatgaggtgc
     3121 ctcctattcc tataaatcta tctcttcttt aatagtatga gaaaagtcta ctcttgtaga
     3181 tttaaattgt caacaaaaaa tttacagatg taaatgataa gttgaataga gaaagataaa
     3241 ttattttttt ggtattcata taaggttaat tatgtattat cacaaaatga ttgttgtcga
     3301 ataactaaaa gtgtcaaaac aatgatgaaa tcatttagtt cttataaatt agacaagaga
     3361 aaataaatat tccatatcta ctttattgga tccataacat gactaaattc tgaatacttt
     3421 ttaaagctta tcatttggta aaactgaata taagttaatt atatagactg aaaattttga
     3481 attttcagaa tagaataact taaaagtact gacaaatttt ttaaaggggc aatattaatg
     3541 ttctgagata atcaaatctt accgaaaata tcaaaagtcc aatgtgacac aattggattt
     3601 tgtcacattc gttaaaatcg ctccatcctt agaggctcta ataggaaatc acttttcttt
     3661 tagcgccttt tttctatagc ttgtttaaat gtgacttatt atctgttata atcgtattaa
     3721 gtaacatttg agaggtgtct aaaaatgaaa agcgtggaac cgattcgcga caaaaaaaag
     3781 atcgatgcca tgaaggccat tttggcttcc ggaaaatatg ggcaacgaaa tttggtgctt
     3841 ttttcgatcg ggatcaatac agcctatcga atctccgatt taagacagtt aaaattaagg
     3901 gatgtgctag aaatttcccg tgggcgggtg atcgtcaaag aacgtctggc catgaaggaa
     3961 caaaaaacgg ccaaacacaa ttcggtcttc atttcgaaca aattgcgaaa agtgattctt
     4021 gattatgttc aatccgaatt tcctgagcag ttgcaagcac aagacttcag caagtatttg
     4081 tttccaagtc gaaaaggagc agatacgcct ttgacccgac aaagcctttg gcggatcatt
     4141 catgaagcag gaactgcagt tgggttaaaa gaaattggtc cccattcgat gcgtaagacc
     4201 tttggctatt ttttatacaa gcaaggaacc aaaacagaga tcattcagtc gctgctaaac
     4261 cattcttcac aacgggagac cttacgctat attggaatca cccaggaaga caaagatact
     4321 gcagtaaaga gtttggattt atgacagaat aatgccctat ataatagaag gaaaaattca
     4381 agaggtgaga aaatgaacga agaaaagcag cctaactttc ctgataaata tcacttatct
     4441 cgcaaagagt ctgtgtattt attgaagaaa aacattgtag aactggtgta taatgcgggg
     4501 aaatttgaag ggttaaatac aacgttgtta caaacagaag ggattattaa gtataatcgg
     4561 gccaacaacg tggctgtaga tgatgtcctg accgtcgtta acttaaaaag aggctttgaa
     4621 atgcttttaa atgacgtaca ggagcccctg ttagagacaa gtaagcggat taatcggatc
     4681 gttgcagcag aggatgcgct ttttcccggt gaaatacgaa ccggtggggt agaattatcg
     4741 acgattcaag gaagatatgt gccaccaatg ttaactgaag acgaggtaaa caatcaatat
     4801 ggtgagataa tgaatcaaga gatatcagat acggaaaaag ccctgcgact gtttctattc
     4861 atttcgaaaa atcaaatatt ctggaatggt aatgtgggta cagccttgtt gattgcgaat
     4921 aagattatgt ttagcaacgg tttaggacta ttatcaatac ccgaaaatgt atttatgaaa
     4981 tttaacgaac ttttatcaaa gtattgcaac agtaatctat tagtcaatga gtcaaatatt
     5041 atatcatttt tatatgaaca gtgtatcttt ggtattaatt atttgaaaat aatccaacat
     5101 acaatttaag aggtataagt aaatgatgac tatagacgaa gcaaaacaat tagcaactta
     5161 tttttcagtt ggttcagcgg gatatatttt agactttact aatgtaacgt ggaacatatt
     5221 tacaaagaaa atcgtaggga ttgaaataca aaatcattat gggggctcaa agggagtatc
     5281 ctttcaaaag tttctgcttg acgatcaatt tgatgattcg ttaaagaatc ggttaatcca
     5341 atctttaaaa ggtattatgg atcgagataa aaaatatgaa tctactgaaa tgaatcccat
     5401 agttcagttg ctttttgagg aagtatttaa agaagatact tcaactgcat taagaaaaat
     5461 gaaattttca agcgagattg atttacttta tattcggcag ctacccatta ggattcaaac
     5521 agatattaaa aacaaggatt ttgactctgt tttgacaaaa gcaaatactt taatagatga
     5581 agttctaaaa tttattattg aaaataccga aggaccgagt gttaatatta atttatttaa
     5641 gtcaaaagaa ttaagaaaag aagtatttgg ccgcctcaat attaaaactg aaaaagatat
     5701 ggataataga atcaaagccc ttgttggagc actcaataca ttatcagata aaatactcga
     5761 aatgagaaat agtcaaggag atgctcatgc acatggctca aatagaatag taattaagga
     5821 agaagaagca attcttgttg caaattcttc tatggttttg tgcgagtatg tgtttactaa
     5881 gtataaagga ttaaatagat aataaaatgc atattttata gtatacaatt cgaattttta
     5941 tgttatatac tataatataa aatcaatata tgatataatt gggtatggaa aaaatatctt
     6001 ttgaaacatt taaaagaccc aatggtcatg atgaattttt agaatggttg gaaacattac
     6061 caaaaaaaga tagtgcgaag cttttacgta caatagaaga aacagaaaag aatgggatgc
     6121 tagtagctca acggttgaaa tgggtgaaaa aattagacac taatttgtac gaattaagaa
     6181 gtaaggtagg atcaaacatc caaagagcca tctatttcca tgtggacaat ggaaggtatg
     6241 tcattactca cggatttact aaaaaaacgg agaaaacacc aataactgaa ctcaagcatg
     6301 caaaagaact tagagaggag tggttcacaa atgatgatta aaaaattgat tgaagaaaag
     6361 aaaaaagata aagattttgc agaagcatat gaccgtgaag gaagaaaact cgaaactgcg
     6421 gttgctcttt atcatgctcg ggaggaagca gggctaactc aagcggagct tgcacaaaaa
     6481 gcacatacaa ctcaatctac tattgctcga attgagagag gggataacgt atctttcgat
     6541 aaacttgatg atatcgccag ggcattaggt aaaaagctgg tagtagaatt tgcttaaaag
     6601 cgagttgaaa atatatctat aataggctcc taactaatca attagtttag tgggcctttt
     6661 tttactagat agataaaggt tgtactatag tgtaaaagac aataaatttt atactaaaat
     6721 tactttaaag agaactagag agaaaggtac aataaatgaa aaccataaat cttataaaag
     6781 tatttatagc atcaccaggt gatatagcag caaaaaggaa tgaagtagaa gaaaccatat
     6841 atagatggaa tatagaaaat acagataata caaaagtgat tttgatgcct atacgatggg
     6901 aaactaatgc gattcctact tatgtaaata atagctctgg gcaaagtgtt attaataaac
     6961 aaattgtaga aaatagggtt ctgctgcaaa gttttaaaaa taaaatcagt ttttgtatta
     7021 tttcttcatt ttaagctgga attcccaata atatcctgat ttcagtgcag actgaaaagc
     7081 cgaagagagt gccttctttt cggcttttct tgtagattcc tcgaatggtt tccatccctt
     7141 taattgtggt ggaagctgta cgtagacttt gatacaattt atgtcgacgt tttatcgatc
     7201 gatgatcttg ttctattaaa ttgttaa
//
LOCUS       DACDOA010000118         7222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDOA010000118 DACDOA010000000
VERSION     DACDOA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.119"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..580
                     /locus_tag="IWI39_002439"
     CDS             <1..580
                     /locus_tag="IWI39_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9209493.1"
                     /translation="IPNYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVK
                     SVASGEGVIHALNTMRLDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLE
                     TGIEEYNVLTAKDERVCRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKL
                     GKDIDEEYDRLFGDLLDEFAHDSFGINLKRRK"
     gene            582..899
                     /locus_tag="IWI39_002440"
     CDS             582..899
                     /locus_tag="IWI39_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209494.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            948..1649
                     /locus_tag="IWI39_002441"
     CDS             948..1649
                     /locus_tag="IWI39_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9209495.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            1664..2554
                     /locus_tag="IWI39_002442"
     CDS             1664..2554
                     /locus_tag="IWI39_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9209496.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            2577..2792
                     /locus_tag="IWI39_002443"
     CDS             2577..2792
                     /locus_tag="IWI39_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209497.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            2804..3145
                     /locus_tag="IWI39_002444"
     CDS             2804..3145
                     /locus_tag="IWI39_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209498.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            3145..3516
                     /locus_tag="IWI39_002445"
     CDS             3145..3516
                     /locus_tag="IWI39_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209499.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            3509..3904
                     /locus_tag="IWI39_002446"
     CDS             3509..3904
                     /locus_tag="IWI39_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9209500.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            3906..4283
                     /locus_tag="IWI39_002447"
     CDS             3906..4283
                     /locus_tag="IWI39_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209501.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            4284..4892
                     /locus_tag="IWI39_002448"
     CDS             4284..4892
                     /locus_tag="IWI39_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9209502.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            4892..5233
                     /locus_tag="IWI39_002449"
     CDS             4892..5233
                     /locus_tag="IWI39_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209503.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            5475..>7222
                     /locus_tag="IWI39_002450"
     CDS             5475..>7222
                     /locus_tag="IWI39_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9209504.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWE"
BASE COUNT         2621 a          957 c         1488 g         2156 t
ORIGIN      
        1 tataccaaat tatttaccag aaaatgaaat taaacattat ttgaactatc catggtcagg
       61 aaagacattt tcaagaagac tttggggaaa tatttcatcg cttgaacaga aactctctaa
      121 tgctattgta aaaagtgttg ctagtggaga aggcgttata cacgcattaa acaccatgag
      181 attagactca gaaatttgtg acatgtttaa gttagaagaa tcaaagtata acaaagcgat
      241 agaaaatctc gttcgaacgg agtatgcaaa atttgcacaa gatggtattg aaaaatcata
      301 tttagaaaca ggtattgagg aatacaacgt attgactgca aaagatgaga gagtttgccg
      361 gatttgtgga ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg tcataggtga
      421 aaatcgagca cctttccata gtcgttgtag atgtacggat gttcctaatt taccaaaatt
      481 aggaaaggat attgatgaag aatatgaccg tttatttggc gatttattag atgagtttgc
      541 acatgattct tttggaatta atttgaaacg gaggaagtag aatgaaagat ttttttgaag
      601 cagtactaac aattaatgta aatgctgata ttgcagaagc ctacaaaaca gctattgagt
      661 ctgagaacca tcctaatggc ttgagagacc attggaatgg caattatgcc tacgtggtta
      721 ttggcgatca aactgttaat tatcaagata atactccagt tgataagaat accgttaatt
      781 taacgattca attattatct cattcattac caaatttaaa agaaacagtt gattggtatg
      841 aaaagatggg atgtattgtt gttagaactg actacaaaga aggaaagtct agtaattagg
      901 cttttttatt ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg gttgaatggt
      961 ggggcgcaac tattaaaact caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa
     1021 ggagaatgag catgaaacac aaatcattaa tgccaatgaa tttgcaatac tttgccgaag
     1081 gtgacgatca aaagttttct tttgatgact tcaaatcttt tgtggaatcc aatgaagaag
     1141 ctcaaaaatt tattcagtca caatctcaaa gtgttgccga caaacaattg gaagcttgga
     1201 aacaaaataa cttagataaa attaaacagg ataccatcaa ggaatatgag gaatctaaga
     1261 aaaacaagtc acctgagcaa attcaactgg aaaaactaca agctgaattt gaagcagaaa
     1321 aagcgttgcg tgtgacaagt gataataaag catttgttgc agaacaaatt gctggattag
     1381 aactagatgg agagttaaaa gagtctattt ctcaatttat gctaaataat cttgttagtt
     1441 cggatacaga tttcactaag aatgctgttg aaggttttac aagtgttttg aatgcaatta
     1501 atgagaaaca tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc
     1561 aacaatcgaa taaccaggtt cagcagaaca atgaaacatt aacaaatcca gaagaacaat
     1621 taggacaaat tcttcaacaa tttaactagg agagtgaaaa attatgaaaa aaacatcttt
     1681 aaataattta gagtatttgg atatttcacc agcgattaat gctatgcaag taccaaatac
     1741 acctttttta agctatttac ttggtgctgg aaaaacagag ccagcaaact cgacagaaat
     1801 taaatggcgt gaatatgata tcaacaacga tgattcttct gaaaaacttg agggcggaga
     1861 atatccagat gctgaatcag gtcgaacttg gtttaacaac tatactgaaa tttttagaaa
     1921 atcaacctct gtatctggta cattagatgc tattaatgtg aatggagtcg gaaatgaatt
     1981 aactaatcaa gtagcactac gtggtatgga aatgaaaatt gatttgaacc gaaaattgat
     2041 tactggtgta aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa
     2101 cttgatcaat tcagcaaata aggcagaaac agccactgcg ggtgcagtaa caagaaaaga
     2161 tatcgatact ttatttaaat tgatgtatga aaaaggttat atgggagaaa aactatgctt
     2221 gatttctcca gatatgcagg agttaatgac tgatgagtta gatggaaaat caacaaaaat
     2281 tgttcagttc ggggaaagag taacttttgg attgcaaatt ggaaatatcg tgtctaatta
     2341 cggtacaggt attgctctac tagaaccatc attgccaaaa ggaacaattg ccgcaatcga
     2401 tactaattat gtgaaactac gtccattacg tgaatggaga gcagaagaac ttgcaaaaac
     2461 aacggattct agacgtatcg gtcttgtagg tgaatactct cttgaataca atgcttcaaa
     2521 ctctggggca attttaaatt taaagtctga ataaaaggga gttagtactc ccttttttga
     2581 taggaggaat tacagtggta aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata
     2641 gagtaggtaa aactaaaaat tttgttgggt ttgttcatcc taaaactcgt agatttatca
     2701 cagcagattc aaataacgaa tttatcattt ctatagatga taaagaagca attgcaattt
     2761 tggaagatgc aattgatgtt aatgaaattt aggaagtgat ctgatggatg aatcgctaaa
     2821 aacggaaatc attgagtcta caaaagaaga ttttccagat ttgagtgaag aacgcataac
     2881 taatttatta gaaataattt tgctagaaat tgaatcatac aacacttgta aaaatgatat
     2941 ttcatgggaa aagttaaaaa gcgtgattag tgaagtgttg tataaaataa taaaaaatga
     3001 atcagaaaaa acagtatctt cagttagacg tggcgataca acgattagtt atgcttcaac
     3061 aacaaatgat gttagtgagt tacttctagg ctatggtgat ttgatacgaa gagttattgg
     3121 ttgtggagga ttggagtttt tttaatgaat gaagcagata ttttagaaac tacttatgaa
     3181 gatagttgta ttattgaaag actgacggac attgaagatt ctaatacaaa tattactatt
     3241 caagattaca aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc
     3301 gatggactag cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa
     3361 ttatttgttc atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag
     3421 gccagtggct taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt
     3481 gaagtgaatt tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga
     3541 aaagatcatt gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt
     3601 cgttaacgat tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc
     3661 aggtaattta aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat
     3721 tgttggagaa gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac
     3781 taaaaatggt ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa
     3841 tatgtcttat attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa
     3901 gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg
     3961 atgggacaat atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat
     4021 cagtaatgga atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa
     4081 ttgatattgc attgattgat aataacccta atagaaattt aattaatgaa ttagtctcac
     4141 gttgtgggac tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct
     4201 atttacctga tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat
     4261 atattgaatg gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg
     4321 tcgaaaaacc tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta
     4381 ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac
     4441 aagattatgg ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg
     4501 aactggaagc aaatggatat actcataaag ttttatctac aattactgga gggaaaattg
     4561 ttaaaggtgg tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata
     4621 gacgtaaaaa atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc
     4681 tagattcaga tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt
     4741 ggaagggttc atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg
     4801 atgaaggcgt ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg
     4861 aagaagcaga actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt
     4921 aacgaaaaaa gacgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt
     4981 accaggaaca gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga
     5041 taagcaatca gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt
     5101 tgagggacaa tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc
     5161 attgacagga aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac
     5221 gaaaaaaaag taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga
     5281 gaattagata taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt
     5341 actgacttgg atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa
     5401 gatgttgagt actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg
     5461 acctaattct gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg
     5521 tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt
     5581 cattaggtag tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa
     5641 tcgtaggagc tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag
     5701 atacgatgaa taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta
     5761 tgaagaatat cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt
     5821 tgaatgatgc gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt
     5881 caactcaaat atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc
     5941 aagttaatga agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac
     6001 aaacttggaa ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa
     6061 agatgggaat tacaatggga gatctgaaag aaggtttatc tcaaggtaag atttctgttg
     6121 aagaattcca aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat
     6181 taagtcaaat cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa
     6241 cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt
     6301 cagatttagg tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct
     6361 taaaagtaat tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg
     6421 aaattcaaaa atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta
     6481 ccgcttttgg agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca
     6541 ataattttaa gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag
     6601 ctgctgctaa tccatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg
     6661 gttactttat ggcaaccaat gaagagctta gaaacaaggt tatatctatt tggaatgatg
     6721 tgaaagattc cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga
     6781 attctgctaa agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa
     6841 caaaggaatg gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg
     6901 ataaaacagt agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata
     6961 tgaagaaatg gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga
     7021 atggaatcaa aggttctatt atgtcagttg ctggtccatt gattacaggt ataaagaatg
     7081 catttttaca tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta
     7141 aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca
     7201 tgatttctgg tggatgggaa ga
//
LOCUS       DACDOA010000119         7140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDOA010000119 DACDOA010000000
VERSION     DACDOA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.120"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..614
                     /locus_tag="IWI39_002451"
     CDS             <1..614
                     /locus_tag="IWI39_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9209505.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="IWI39_002452"
     CDS             611..1384
                     /locus_tag="IWI39_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9209506.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="IWI39_002453"
     CDS             1381..1779
                     /locus_tag="IWI39_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9209507.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="IWI39_002454"
     CDS             1964..2635
                     /locus_tag="IWI39_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209508.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="IWI39_002455"
     CDS             2694..3134
                     /locus_tag="IWI39_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209509.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="IWI39_002456"
     CDS             3146..3814
                     /locus_tag="IWI39_002456"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9209510.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="IWI39_002457"
     CDS             3878..4501
                     /locus_tag="IWI39_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9209511.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="IWI39_002458"
     CDS             4557..4943
                     /locus_tag="IWI39_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9209512.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="IWI39_002459"
     CDS             5156..6547
                     /locus_tag="IWI39_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9209513.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2131 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     6721 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
     6781 tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt tcactctgag tcacaaagag
     6841 caatttcaaa aaatagcttc tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg
     6901 tcaggagaac accgtttacc agaggttgtg acttgttttt agtcacgacc tcttttttta
     6961 tttattccat ataaatacac attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac
     7021 aaccaactta tttattatta aagaaaagcg tatttaagta ggacgattct gttgagagct
     7081 ttcgtagatt ttcccatttt cctatggtaa actgacaaag ttgagaatgt tagaacagat
//
LOCUS       DACDOA010000120         7027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDOA010000120 DACDOA010000000
VERSION     DACDOA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.121"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(158..616)
                     /locus_tag="IWI39_002460"
     CDS             complement(158..616)
                     /locus_tag="IWI39_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9209514.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1954)
                     /locus_tag="IWI39_002461"
     CDS             complement(629..1954)
                     /locus_tag="IWI39_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9209515.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3383)
                     /locus_tag="IWI39_002462"
     CDS             complement(1971..3383)
                     /locus_tag="IWI39_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9209516.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="IWI39_002463"
     CDS             complement(3400..4164)
                     /locus_tag="IWI39_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9209517.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="IWI39_002464"
     CDS             complement(4268..5143)
                     /locus_tag="IWI39_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209518.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="IWI39_002465"
     tRNA            complement(5398..5482)
                     /locus_tag="IWI39_002465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="IWI39_002466"
     tRNA            complement(5500..5571)
                     /locus_tag="IWI39_002466"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
     gene            complement(5581..5653)
                     /locus_tag="IWI39_002467"
     tRNA            complement(5581..5653)
                     /locus_tag="IWI39_002467"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5715..5799)
                     /locus_tag="IWI39_002468"
     tRNA            complement(5715..5799)
                     /locus_tag="IWI39_002468"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Leu,seq:caa)
     gene            complement(5820..5890)
                     /locus_tag="IWI39_002469"
     tRNA            complement(5820..5890)
                     /locus_tag="IWI39_002469"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Cys,seq:gca)
     gene            complement(5936..6007)
                     /locus_tag="IWI39_002470"
     tRNA            complement(5936..6007)
                     /locus_tag="IWI39_002470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5973..5975),aa:Gln,seq:ttg)
     gene            complement(6019..6091)
                     /locus_tag="IWI39_002471"
     tRNA            complement(6019..6091)
                     /locus_tag="IWI39_002471"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6056..6058),aa:His,seq:gtg)
     gene            complement(6094..6167)
                     /locus_tag="IWI39_002472"
     tRNA            complement(6094..6167)
                     /locus_tag="IWI39_002472"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6133..6135),aa:Trp,seq:cca)
     gene            complement(6174..6254)
                     /locus_tag="IWI39_002473"
     tRNA            complement(6174..6254)
                     /locus_tag="IWI39_002473"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6218..6220),aa:Tyr,seq:gta)
     gene            complement(6256..6331)
                     /locus_tag="IWI39_002474"
     tRNA            complement(6256..6331)
                     /locus_tag="IWI39_002474"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6296..6298),aa:Phe,seq:gaa)
     gene            complement(6367..6439)
                     /locus_tag="IWI39_002475"
     tRNA            complement(6367..6439)
                     /locus_tag="IWI39_002475"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6404..6406),aa:Asp,seq:gtc)
     gene            complement(6449..6521)
                     /locus_tag="IWI39_002476"
     tRNA            complement(6449..6521)
                     /locus_tag="IWI39_002476"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6486..6488),aa:Val,seq:tac)
     gene            complement(6537..6608)
                     /locus_tag="IWI39_002477"
     tRNA            complement(6537..6608)
                     /locus_tag="IWI39_002477"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6573..6575),aa:Glu,seq:ttc)
     gene            complement(6623..6712)
                     /locus_tag="IWI39_002478"
     tRNA            complement(6623..6712)
                     /locus_tag="IWI39_002478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6676..6678),aa:Ser,seq:gga)
     gene            complement(6752..6825)
                     /locus_tag="IWI39_002479"
     tRNA            complement(6752..6825)
                     /locus_tag="IWI39_002479"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6789..6791),aa:Asn,seq:gtt)
     gene            complement(6835..6950)
                     /gene="rrf"
                     /locus_tag="IWI39_002480"
     rRNA            complement(6835..6950)
                     /gene="rrf"
                     /locus_tag="IWI39_002480"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2117 a         1553 c         1150 g         2207 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
     5581 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5641 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5701 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5761 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5821 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5881 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat agaaactggg
     5941 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     6001 taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     6061 tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca
     6121 cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga
     6181 gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac
     6241 ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa
     6301 tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa
     6361 gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     6421 acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc
     6481 tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac
     6541 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     6601 aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc
     6661 accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa
     6721 agaagctcaa tactctttct caaaatgaga actccggcag taggactcga acctacgaca
     6781 tcatgattaa cagtcatgcg ctactaccaa ctgagctatg ccggaataac atcagcgtgg
     6841 cgacgtccta ctctcacaag gggcaacccc tcactacaat cggcgctaag aagcttaact
     6901 tctgtgttcg gcatggttac aggtgtatcc ttctcgctat cgccaccaca ctgtggtgtt
     6961 atcttttatt gagtaaattt tgttcactca aaactggatt tgaagtcaca taagtttttt
     7021 tccgagt
//
LOCUS       DACDOA010000121         6959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDOA010000121 DACDOA010000000
VERSION     DACDOA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.122"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..2516
                     /locus_tag="IWI39_002481"
     CDS             144..2516
                     /locus_tag="IWI39_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209519.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2577..4037
                     /locus_tag="IWI39_002482"
     CDS             2577..4037
                     /locus_tag="IWI39_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9209520.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4048..6252
                     /gene="topB"
                     /locus_tag="IWI39_002483"
     CDS             4048..6252
                     /gene="topB"
                     /locus_tag="IWI39_002483"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9209521.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6492..>6959
                     /locus_tag="IWI39_002484"
     CDS             6492..>6959
                     /locus_tag="IWI39_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9209522.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYE"
BASE COUNT         2654 a         1170 c         1492 g         1643 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaag aacatggcag aagaaaaaga accactagac aatagtcgtt
      181 tgggaaaatc caaaagaaaa ctagtacgat tgcaaaatga attaaacgaa caaattgaaa
      241 agatgtttga acaccaaaaa atgacgaacg gtcaaccaat gaacgacaag aggaatggcc
      301 gttcatggtt taaccagcaa gaacgaatcg aaaataaagt ccaaagtctg agagaagaga
      361 tcaagcaaca agaagaatac gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg
      421 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
      481 aaatcgaacg ctttgaaaaa ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc
      541 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
      601 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
      661 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
      721 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
      781 aattacttga agaggctaga ccaactgatt ctatgaagga gaaagaaaac gatcatttct
      841 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
      901 tcgtaacatt cgaattgatc gatcaactca aaacattgga taaccaatac gccgcagaag
      961 caggttatta taaattctat tttgaggaag tgaagaacgg agaagtagtc gatcagatcc
     1021 gaatggatct aggggatggc gtcaacagta atcaaccaat ctatttagag ttagcaaaat
     1081 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
     1141 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
     1201 cagaagagat taaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
     1261 agcataccca ttctcctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
     1321 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
     1381 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
     1441 agatctttgt cagaaaaacg gtcaaagggt tttatgagaa aggacgtggc tttgttcgag
     1501 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
     1561 aaaaacctta ctatacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
     1621 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
     1681 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
     1741 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
     1801 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
     1861 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
     1921 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
     1981 gaatggacac gtcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
     2041 cgttggatga taaggaagct gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
     2101 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
     2161 agatccaacc agcagaagtg aagcaggagc ataaaaatac tttagaaacg ccagagaata
     2221 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
     2281 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
     2341 atcaagtaat tttaaaagga aaaagttatg gaaaagagat ccaaacggcg ttaccttcag
     2401 aagaatttgc gctaggaaat ccattccgat tttcacttga gacagtgtca gaagtggcaa
     2461 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgt gtataaaaca
     2521 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt tttaaaatga
     2581 ccgagaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
     2641 gaaacaatgg aatggctgta gtcaataaag gacgagatta tcgcttagaa gaccatgatt
     2701 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
     2761 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac cctaagcaac
     2821 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
     2881 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
     2941 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
     3001 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
     3061 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
     3121 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
     3181 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
     3241 gtgaccctaa gcagataaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
     3301 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
     3361 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
     3421 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
     3481 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
     3541 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
     3601 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
     3661 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
     3721 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
     3781 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
     3841 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
     3901 atcatgaaca tgaatttgta cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
     3961 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
     4021 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
     4081 caagcgaaag cttatgccga tagcttctct aaagccactc gaaaagacgg gtattttgaa
     4141 atccaagatc gattgtttcc tggtgaaact gtgatcacgt atggttttgg tcatttagtc
     4201 gaattagatt cgcccgatat gtacgatgaa aattggaaac aatggtcatt agaacatttg
     4261 cctattttcc ctacacatta tcactatcat gttcctaaag ataagaaaaa gcaattcaag
     4321 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
     4381 gaaggggaac tgatcgcttg gacgatcatt caacaagtag gcgccgatca aggaaaaacc
     4441 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccagcag
     4501 ttacgagacg caaaagagac ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc
     4561 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
     4621 ataccaggaa gcttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcaa
     4681 ttgcaggaaa agattagaaa tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta
     4741 atcgctcaaa atggcgcatt tgatgcgaag cttgatccga atgaaaccca agcgacgcaa
     4801 gaagcatttg aagattattt aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg
     4861 attcgccaag ttgaaaccga gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc
     4921 agccttcagt ctaaaatgaa tcagctcatg aaagctagtg ccaaagacac tttggaagcc
     4981 atgcaaggct tgtatgaagg gaaatatcta agctatccgc gaacggatac cccttacatt
     5041 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttgagg
     5101 gctgaagaaa tttcgactcc tattcataca ccgaatagta ggtacgtgaa caacaagaaa
     5161 gtccaggaac attacgcaat cattcctaca aaaacagtta tgacagaagc gacgtatgaa
     5221 agtctgacac ctttacaaca agcgatttac gagcaagttt taaaaacgac agtggcgatg
     5281 tttgcggaaa aatataccta tgaagaaaca acgatcttga cgcaagtcca gcagcttcag
     5341 ttaaaggcaa tcggaaaagt accgatagat ctaggttgga agaagttatt tggcaaagag
     5401 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
     5461 gtgacggtag atctacaagt ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa
     5521 ggtacgttga tcactgcgat gaaaacagct gggaaaaccg tggatagtga agaagcgcaa
     5581 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
     5641 accttgaaac aaaaggaata tatcaaggta gagaaaaata agcttgtggt gacgaataaa
     5701 ggggttttgc tttgccaagc tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
     5761 gccaaatggg aaagctactt gttaaagatt ggagaacgaa aaggaacaca aaccactttt
     5821 ctagccaata tccaaaagtt tgtctctcac ttattggaag tcgtcccagg acaaatccaa
     5881 tccattgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
     5941 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
     6001 gtggcagctt gtacgtctga agcctgtgat tttaaattat ggacaacgat tgccaaaaag
     6061 aaactcacag cgacacagct gaaggagatt attcaaaata gccgaacaag ccaaccagtg
     6121 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
     6181 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
     6241 acgacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
     6301 gaaaatcaaa aagccaaaat tcgttcagaa caagagaaat tacgtgccag taaaagaaga
     6361 cttcagcaat taaagaacca gcaacggatt caaaaagaaa tcgaattaga tcgatagaca
     6421 ttaaccttgt tacaaataca ctatcatgat atatttaatg tagtgtattt gtaacaaaag
     6481 gggggaagta aatgcaagag aaactgaaag aattgttggc tattcgcgat ggaaatttat
     6541 ctatgaagga agccagaaaa ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc
     6601 aaggaaaatt gatcaaagtt gatcgtggtt attatgtaga ggatggtcat ggaatagatg
     6661 atttattttg gcttcagcaa cgtttttcca ggggaatctt ctctcatgaa acgactttag
     6721 atatttacca gctttctacg aatatgccga aattcattca cttaactttt ccccatggat
     6781 ataacgttga tcgttcaatt acaaaagagc agcagttaca atttcatttc gtgaagaaag
     6841 aagtttatga actaggaaaa gtagagggaa cttcctttca aggaaatcct attactatgt
     6901 atgataaaga gcgtacactt tgtgatattt ggaatccgag atatgaaatt gaatatgaa
//
LOCUS       DACDOA010000122         6927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDOA010000122 DACDOA010000000
VERSION     DACDOA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.123"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            20..409
                     /locus_tag="IWI39_002485"
     CDS             20..409
                     /locus_tag="IWI39_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9209523.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            396..2843
                     /locus_tag="IWI39_002486"
     CDS             396..2843
                     /locus_tag="IWI39_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9209524.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            2848..4956
                     /locus_tag="IWI39_002487"
     CDS             2848..4956
                     /locus_tag="IWI39_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9209525.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALEVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            4953..5957
                     /locus_tag="IWI39_002488"
     CDS             4953..5957
                     /locus_tag="IWI39_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9209526.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            5976..6887
                     /locus_tag="IWI39_002489"
     CDS             5976..6887
                     /locus_tag="IWI39_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9209527.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
BASE COUNT         2240 a         1174 c         1530 g         1983 t
ORIGIN      
        1 ttttattttg gaggaaacta tgaaaaaaat aaaaagctac accagtattt gggcagttga
       61 aaaagtcatt tatgcgatta atgactttca gctgcctttt cctgtgacct ttaaccagat
      121 ggcttggttt gttttatcct tgatgtttgt cattgtattt gcccatgtgc ctccgctatc
      181 catgatagag ggggcttttt taaaatactt gggcattccg atagcggtca cttggttcat
      241 gtcgcaaaag acctttgacg gtaagaaacc attgggattt ttacgatcct ttatcagcta
      301 tcacttacgg tttaaggtca cttttgcagg aaagaaagtt aaagaacaga agaaacgatg
      361 ggacgaacca atcaccttgg taaggagtgt gaattatgta cccgattaag tatattgaaa
      421 ataatcttgt ctttaaccaa gaaggcgagt gttttgctta ctatgaatta gtgccttaca
      481 attactcctt tttgtcacct gaacagaagt atcaagtaca tgataacttt cgccaattaa
      541 ttgcgcaaaa tcgtgaaggt aagatccatg ccttacaaat tgccacagaa agtagtattc
      601 gtgccacaca agaacgttcg aaaaaggaaa ttaccggtag actcaaagaa gtagcaaagc
      661 aacgaattga tttacagaca gatgcattag tatccatgat tggcgatagt cagattgatt
      721 atcgcttctt tattggcttt aaactgattt cgacagatga agaggtcaat ctaaagaatc
      781 tcaggaaatc tttcttttct ggacttcaag aatttgtcta tggagtgaat catcatttga
      841 tgggtgattt tgtttcctta tccaatgaag aaattcgtcg ctataccaag cttgaaaaac
      901 taatggaaag taaattggct cgaagattta aagtcaaacg agtaacgccg agtgacttga
      961 cgtatttaat tgaacatatc tatggtgaaa aaggcacacc gtttgaagaa tatgaattcc
     1021 agctaccaaa gaaaaagctg aaatcagaaa cgctggtgaa acgttacgac ttattacgtc
     1081 ccactcgttg tttgattgaa gaaaagcctc ggtacttacg gatggaacat gaaaatcatg
     1141 agtcttatgt agcgtactta acaattaata ccattgtagg ggaaatggag tttccttcct
     1201 cggaactttt ctattaccag caacaacaat ttacgtttcc gattgatact tcaatgaatg
     1261 tggaaattgt gacgaataaa aaggccctag ctaccgttcg taataagaaa aaggaattaa
     1321 aagatttaga caaccatgcc tatcaatctg ataatgaaac gaactccaat gtattagatg
     1381 ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcgaaagaa tccatgtata
     1441 aactaagcta tgtggttcga gtgagtgcta aatcagtgga tgaattaaaa cgccgctgtg
     1501 atgaagtgct cgatttttat gacgatacca atgtaaaact agtccggccc tttggcgata
     1561 tgatgggtct acacaaagaa ttcttaccct caagtaagcg atacatgaat gactatattc
     1621 aatatgttac gtctgacttt ttggcgggac ttggttttgg ggctactcaa atgctaggag
     1681 aattagaagg catttacttt ggctataacg tagatacggg gcgcaatgtt tacctgaaac
     1741 cggcattggc atcacaaggg gtcaaaggct cagtaaccaa tgctttggca tctgctttcc
     1801 ttggttcgtt aggtgggggg aaatctttta gcaacaatct cttagtctat tatgccgtcc
     1861 tttttggtgg gcaagctgtg attgtcgatc caaaaggaga aagaggcggt tggaaagaaa
     1921 cgttgccaga aattgcggat gaaattaata ttcttaactt gaccagtaag ccagaaaatc
     1981 aaggcttact tgatccctat gtgattatga agaaaaagaa agactcagaa agtttggcga
     2041 ttgatatcct gaccttttta acagggattt ccagtcggga tggggagaag ttccctgtgt
     2101 tacgcaaagc cattcgaacg gttacccaaa gtgatcagcg aggattatta caagtaatcg
     2161 ttgaattgcg taaagaggac tcaccagtcg cggaaaatat cgcggatcac atcgagtcct
     2221 ttaccgatta tgattttgcc catttgcttt tctcagatgg ttcgattacc cagtcgatta
     2281 gtttagataa gcagttaaat atcattcaag tagcggattt ggttttacca gatgcagaaa
     2341 ccacttttga agaatatacc accatggaat tgctgagtgt ttccatgcta attgttattt
     2401 ccacttttgc cttggacttt atccatagca accgagaaat tttcaagatt gtggacctgg
     2461 atgaggcttg gagttttctt caagtagctc aaggaaaaac gttatcgaat aaattagtgc
     2521 gtgctggtcg ggccatgaat gccggtgttt acttcgtcac gcaaaacagt gatgacttgt
     2581 tggatgaacg tttgaagaat aatattgggc taaagtttgc atttcgcagt accgatattc
     2641 atgaaatcaa gaaaaccttg gaattctttg gattagataa agaagatgaa agtaatcaaa
     2701 aatgcatgcg agatttggaa aatgggcaat gcttgatgca ggatttgtat ggtcgagtag
     2761 gtgtggtcca agttcatcca gtctttgaag aacttttcca tgcttttgat acccgtccgc
     2821 cagttcaaga gagaatggag tgaggagatg aaaaagaaac tttttcgtat ctttggaatt
     2881 gttttaatca gtttggtcac tctgataata cttttgagct tagttggaac ggtagctgag
     2941 gcaacaggtc tggttgatga tacagttgga gccggaaatc tttattccca atattcactt
     3001 ggtaactatc agttggattt ctttgtggat agttcgtggg attggctgcc ttggaattgg
     3061 ggtgatggcc tagggaagag cgtaatgtat ggactttatg ccattactaa ttttatttgg
     3121 acagttagtc tctacttatc aaatgccacg ggctacgtgg ttcaagaagc ctataagctg
     3181 gatttcattt cagatacggc tgaaagtatt gggaagaata tccaaacctt agccggcatt
     3241 accgaaaatg gactccagac ctcgggcttt tactttgggt ttctattatt gatgatttta
     3301 gctctagaag tttatgtggc gtatacagga ttattaaaac gagaaactac caaagcagtc
     3361 cgggctgtca ttaattttgt gatgattttt ctactttcgg gttcgttcat tgcgtatgcg
     3421 cccacctata tcacaaaaat caatgatttt agctcagatg tcagcgaagc ggctcttagc
     3481 cttggaaccg agattgtcgt gccgaattca gaaagccaag gaaaggacag tgtggattta
     3541 atccgagata gcttgttttc cattcaagtc caacaaccgt ggttactctt gcaatttgat
     3601 gattccaata tagaagaagt tggcgaagat cgtgttaaca aaatcctgtc tgtaagtcca
     3661 gatgaaaata aaggcaagga tcgggaagaa gcggtcaaag cggagattga agataatgac
     3721 aatgcgaatc tcagtattac caagaccatg aaccgcttag ggaccgtggt ctttttggtg
     3781 ttgttcaata ttggcatatc cttctttgtc tttctcctaa cagggattat gttgttctcg
     3841 caaattctct ttattatctt tgcgatgttt ttgccgatta gtttcttatt gtccatgttg
     3901 ccgacttatg agagtttggg caagaaggcg attattcgct tgttcaatac gattatgatg
     3961 cgagcaggag ttactttggt gattacgact gcatttagca tctccacgat gtttttcaat
     4021 atttcggcaa cctatccatt tttcatggtt gcttttctgc aaattgtgac ctttgccggt
     4081 atctatttca aattgggcga cattatgaat atgttcaatc tccagagtaa tgatagccaa
     4141 tcaatgggaa gacgggtcat gcgaaaaccg caaatgttaa tgaatcgaac attgcggcaa
     4201 ctcaatcgaa atgtgggacg gactttggct tttggtggtg gtgcagcagt tggaaataag
     4261 ttggcaaagg aacaatcaaa atccaaattt aagccatccg gttcttctct acgaaagaat
     4321 tcacgattac cgaacgatca cgagatacca tctgacttga ataaggaaaa gccaattcca
     4381 aataataaga aacaatcccg aatgaatttg gccgggagaa aagtcggcaa ggttctggat
     4441 acccaagctt tggtgaaaga taagaccaaa caagtaaagg atggagtacg gaatacgcca
     4501 accaacttga aatacaactt ccataaagga attgaaaaaa cgaaaaaagc accggaagag
     4561 tttaagcgag gactggtcca agaaaaagca aatcgaggag agttgcgaga aaaacaacaa
     4621 caacgtagag acgaaaaaat agctgaaaag cgcaaagtat tgaacgaaat agggaatcct
     4681 catgggaaaa gaagggaaaa tgttccgata aaagcaaagg tccaatctca aaaggataga
     4741 gcaccaaagc ggattgtgag agcaaattcg aatcccgaaa tcaaacgaaa attcgctagc
     4801 caagaaatca tttcaaaagg aataaaccaa tcctcaagta agaaaaattc ttttcaacaa
     4861 gtgaaacggc ggtctactct gtccaaacga accaatcgaa aagtacaaaa aagtttgaac
     4921 cctcatccca aaccaaggtc aggtgagaaa aaatgaacgt aaagaaatta agtgccattt
     4981 cgttaacttt cctaacaatc tcttttatgg gtatcttgat gtgtgtgggc ttattgtttg
     5041 gtgaagacag tgaaggaagt agtagttcag gtgctcctcc tggggggagt agtgtctcag
     5101 aagaggtttt gaaacaccgt gcaatggtgg aaaaatatgc caaagaatcg gatataacag
     5161 agtatgttcc gattctttta gcgattattc aagtggaatc tggtggcaca atggaagatg
     5221 tgatgcaaag ttctgagtca gccggtttac caccgaattc attaagtacc gaggcatcca
     5281 tcaaacaagg atgtctctat tttgcgtctt tggttaagcg ttccaaagaa ttaggttgtg
     5341 atcaagatag tatcattcag gcctataact ttggcggtgg ttatttggac tatgtggcaa
     5401 aaaacgggaa gaagcatagt tttgctttag ctgaatcttt ttcaaaggaa aaatcttgcg
     5461 gtcaaaaaat cgattaccct aatcccatcg cgaataagga aaatggtgga tggcgctata
     5521 actatggcaa tatgttttat tgcttattgg tgaagcaata cctaactaca acacagtttg
     5581 acgataaaac agtccaaggg atttttgacg aggcgtttaa gtatgaagga acggcttatg
     5641 tatttggtgg gtcaagaccc gaaacaggat ttgattgtag tggcttgact caatggtgtt
     5701 atgccaaagt tggtctgaaa cttcctcggg tggctcaaga tcagtatgat gccatgaccc
     5761 atattgacct aaaagatgcc aaaccagggg atttggtttt ctttcattcg acttatgatg
     5821 ccggtactta tgtgacccat gtcggtattt atgcagggga gaatcgcatg tttcatgcag
     5881 gagatccagt aggctggacg aatctaacag aaagctattg gcagcagcat cttatcggtg
     5941 ctggacggta caaataagag aaaagaggaa aaagaatgaa gataaaaatt gaacggaatc
     6001 aaaaggaaaa gagcctaaaa aagaaaaaag aacgagtcgt atccgtgggt aagcatcgaa
     6061 aaatggtgct tgccctttgg cttttattat cttgtagtct tacttttggc gtttataaaa
     6121 atttcaccgc cattgatcaa catacaactc atgaaaaagt ggttattaaa gagaaagtag
     6181 tgaatacttc tggtattgaa aactttacga aggatttttc gaaagaatat ttttcgtgga
     6241 aaaataacaa agaagcaatt gaaaagagaa tgaacaacct cgaacagtac cttacagaag
     6301 aaggtttcgc tttaagtcaa gatatggttc gagcagatat tcccactaat tccgaagttc
     6361 agtcagtaaa agtcttagat gttgaaaaga gctcggagga atttgtcgtt tcatttttag
     6421 tggagcaaaa gattacagag ggaaaaaagg cacaatcgat ttcttctgca taccgagtga
     6481 cgatctttga agatgaaaac aggaattata tagtaacgag tttgccaact atgattgcaa
     6541 aacctgatag ggcaaaatac aagacaaaac aagtagaaaa tgattcgaaa attgatgcca
     6601 aaactacaga agaaatcgct gagtttttgg aaacgttctt taaattatat ccatcagctt
     6661 ctgaaaaaga attggagtat tatgttgaaa atgatgcgat gcgaccaatc aatattgact
     6721 taaagttttc gaagttagag gatccagttt acctacaaaa cggaaaaaaa attctagtga
     6781 gtacaaagat aaaatacctt gataatgtat caaaaaccac tagctacttt cagtacaaat
     6841 taatattaca aaaaaatgga aactggaaaa tcattgatgt taaataataa tgactcaatc
     6901 taaatttatg agaagttata agtagtt
//
LOCUS       DACDOA010000123         6810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDOA010000123 DACDOA010000000
VERSION     DACDOA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.124"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..1025
                     /locus_tag="IWI39_002490"
     CDS             168..1025
                     /locus_tag="IWI39_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9209528.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1018..1920
                     /locus_tag="IWI39_002491"
     CDS             1018..1920
                     /locus_tag="IWI39_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9209529.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1953..2303
                     /locus_tag="IWI39_002492"
     CDS             1953..2303
                     /locus_tag="IWI39_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9209530.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2337..3275
                     /locus_tag="IWI39_002493"
     CDS             2337..3275
                     /locus_tag="IWI39_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9209531.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3353..4246
                     /locus_tag="IWI39_002494"
     CDS             3353..4246
                     /locus_tag="IWI39_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9209532.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4284..4847
                     /gene="amaP"
                     /locus_tag="IWI39_002495"
     CDS             4284..4847
                     /gene="amaP"
                     /locus_tag="IWI39_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9209533.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4861..5049
                     /locus_tag="IWI39_002496"
     CDS             4861..5049
                     /locus_tag="IWI39_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9209534.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5062..5964
                     /locus_tag="IWI39_002497"
     CDS             5062..5964
                     /locus_tag="IWI39_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9209535.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5996..6238
                     /locus_tag="IWI39_002498"
     CDS             5996..6238
                     /locus_tag="IWI39_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9209536.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6494..>6810)
                     /locus_tag="IWI39_002499"
     CDS             complement(6494..>6810)
                     /locus_tag="IWI39_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209537.1"
                     /translation="TFFLLVNRLAEQHDTIAALTKLCLLLFVCLDYFTSYYWLKQTRV
                     KKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKVVVLFCMFTFPISRKFREAL
                     FY"
BASE COUNT         2480 a         1166 c         1364 g         1800 t
ORIGIN      
        1 taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt
       61 ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc
      121 tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat
      181 acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga
      241 tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg
      301 acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa
      361 gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca
      421 gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag
      481 aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa
      541 aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag
      601 tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga
      661 tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc
      721 tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt
      781 atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac
      841 gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt
      901 cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat catccgtata
      961 ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg
     1021 aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct
     1081 gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg
     1141 attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca
     1201 cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta
     1261 atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc
     1321 ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc
     1381 tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg
     1441 ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca
     1501 ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta
     1561 gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct
     1621 atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg
     1681 gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac
     1741 aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg
     1801 ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa
     1861 ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa
     1921 tagaagacca caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag
     1981 tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg
     2041 attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca
     2101 ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca
     2161 tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca
     2221 aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat
     2281 taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga
     2341 gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt
     2401 ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc
     2461 ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc
     2521 tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag
     2581 ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
     2641 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
     2701 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
     2761 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
     2821 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
     2881 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
     2941 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
     3001 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
     3061 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
     3121 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
     3181 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
     3241 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
     3301 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
     3361 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
     3421 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
     3481 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
     3541 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
     3601 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg caggacgtaa
     3661 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
     3721 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
     3781 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
     3841 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
     3901 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
     3961 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
     4021 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
     4081 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
     4141 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
     4201 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
     4261 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
     4321 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
     4381 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
     4441 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
     4501 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
     4561 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
     4621 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
     4681 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
     4741 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
     4801 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa
     4861 atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact tggattgata
     4921 ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca
     4981 gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt
     5041 aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac
     5101 aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg
     5161 aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac
     5221 gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca
     5281 ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg
     5341 gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac
     5401 acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct
     5461 gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc
     5521 atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct
     5581 aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc
     5641 ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa
     5701 aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta
     5761 gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa
     5821 gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct
     5881 tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa
     5941 aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg
     6001 atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa
     6061 taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact
     6121 tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc
     6181 attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa
     6241 agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag aacagccaaa
     6301 caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat gcaagctttt
     6361 gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc ctcttattta
     6421 tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc tttattgata
     6481 cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata ggaaaggtga
     6541 acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg atcgtatgat
     6601 actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag acaaggaaga
     6661 aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta aagtaatcta
     6721 gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca tgttgctctg
     6781 ctagacgatt gacgagcagg aaaaatgttg
//
LOCUS       DACDOA010000124         6468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDOA010000124 DACDOA010000000
VERSION     DACDOA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.125"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWI39_002500"
     CDS             complement(99..1229)
                     /locus_tag="IWI39_002500"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9209538.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWI39_002501"
     CDS             complement(1878..3080)
                     /locus_tag="IWI39_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209539.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWI39_002502"
     CDS             complement(3140..3343)
                     /locus_tag="IWI39_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9209540.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWI39_002503"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWI39_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWI39_002504"
     CDS             4880..5692
                     /locus_tag="IWI39_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209541.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1950 a         1316 c         1012 g         2190 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagt
//
LOCUS       DACDOA010000125         6350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDOA010000125 DACDOA010000000
VERSION     DACDOA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.126"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IWI39_002505"
     CDS             290..1135
                     /locus_tag="IWI39_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9209542.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IWI39_002506"
     CDS             1416..2636
                     /locus_tag="IWI39_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9209543.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IWI39_002507"
     CDS             2633..5353
                     /locus_tag="IWI39_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9209544.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IWI39_002508"
     CDS             5337..6281
                     /locus_tag="IWI39_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9209545.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1780 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt
//
LOCUS       DACDOA010000126         6344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDOA010000126 DACDOA010000000
VERSION     DACDOA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.127"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..585
                     /locus_tag="IWI39_002509"
     CDS             <1..585
                     /locus_tag="IWI39_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209546.1"
                     /translation="NKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLLNNLSLIDKQYT
                     GNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKFNSNISNDHIY
                     NLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGNLDKENSTFI
                     MNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1278..1658
                     /locus_tag="IWI39_002510"
     CDS             1278..1658
                     /locus_tag="IWI39_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9209547.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1640..2554
                     /locus_tag="IWI39_002511"
     CDS             1640..2554
                     /locus_tag="IWI39_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9209548.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2794..3483
                     /locus_tag="IWI39_002512"
     CDS             2794..3483
                     /locus_tag="IWI39_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209549.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3604..3690)
                     /locus_tag="IWI39_002513"
     CDS             complement(3604..3690)
                     /locus_tag="IWI39_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9209550.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4335..5072
                     /locus_tag="IWI39_002514"
     CDS             4335..5072
                     /locus_tag="IWI39_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9209551.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5100..5639
                     /locus_tag="IWI39_002515"
     CDS             5100..5639
                     /locus_tag="IWI39_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9209552.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
BASE COUNT         2463 a         1014 c         1111 g         1756 t
ORIGIN      
        1 aataaaaaaa agatcaaaca aaattggaac ttttcaatat cagaaaaaga ttttattata
       61 gtaacaggaa aaagtggtag tggaaaaact actctgttaa ataatttaag cctaatagat
      121 aaacaatata ccggtaactt attctatttt gaaaagaaag tttctaaaca tgatatacaa
      181 cgtctacgaa gaaatcatat tagctattta tttcaaaact atgctcttct tgaaaatcaa
      241 acagtacaat acaatttcga attagcaaaa aagtttaaca gtaatatttc caatgatcat
      301 atttataatt tattaacaga tttttctttg ccaaaagata tcttaaaaga aaaaatcttt
      361 ctcttaagcg gtggagaaca acaaagaata gcattaatta gaaatatgtt aaaaccattt
      421 gatatattat ttgcagatga acctacagga aatttagata aagaaaactc aacttttata
      481 atgaactttc tgaaaaattt aacagaaaaa gaaaaaaaag ccgttgtact agtaacacat
      541 gacttaaatg ttctaaacta tggaactaat attatagaat tataattttt acccgtgatg
      601 ctagctgatt taaaactagc accacgggta tttattttat attccatctc tcctatccca
      661 aaatttaaat aatcacttaa taaaaaggag gcaccctagt gattttattg aaaaaagagt
      721 atgaaaaatt ttatgataga cttatttatt ctttgcaatt gccaaaaaaa ttaaaccgtc
      781 aattacaaaa gacttatgaa actaaatcaa ctgaagaatg ggagagttat tgctagattt
      841 ttggtagaag taacaaaaag tggacatatt atcataatga ctcgccgaaa gtctgataaa
      901 aaattggcta aaaatattgc tgaatgttta ataaaactat cagttttagc taaatagtta
      961 gtagtttaca caaaaaagca tttatgtttt ttgttgcaaa gtaacaattt catcttcggt
     1021 gaaataagcg aaactttaca aaaagtggct agcatttgct agcttttttt gtattgtgga
     1081 gcgtggcttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg
     1141 cactaaatac gtaggtattt ggctagcaaa tatagagctt gccaaaccct tttagtgagc
     1201 tttactcatt gtaccttttt aggtacaatg gtattgcgaa cgatacgtcc gtaggacgta
     1261 ctgaaagggt gggacttatg agcgaacaaa atcaaaattt ggctagcaat tcttctaaaa
     1321 aatataccta tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa tccgaatttg
     1381 caaagctgaa gcaatcggct gaagctttgc aaatgagtgt gccggctttc gtgaaagcca
     1441 aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgcc caggcaatcg
     1501 cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgctaagtgg tgcaatacac
     1561 accaacatga cgttgcttct ggtgacgctc aacgtttatc cgaaaaccta aaaatcatgc
     1621 aaaaggagct acaaaagata tggcaacaat taaggtaagc cgaaccactt cttgtagtcg
     1681 tgcaattaat tacgcagaac cacgagcaac cattaaaaca ggaataaact gtgacgtcga
     1741 ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg accgtgtaca
     1801 ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa aagctaatca
     1861 actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaacagcta tttacacaca
     1921 tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc tggagactgg
     1981 aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc taaatgacga
     2041 gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag aaaaacgaac
     2101 catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg aaattcggat
     2161 tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat tttgtaattc
     2221 cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt acgaattgat
     2281 ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa aggagacgat
     2341 tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca acgagctacg
     2401 aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac aacgaactct
     2461 tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac acgttgaaag
     2521 aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata gaaaacgaaa
     2581 taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa actacgattg
     2641 atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag gaacagaaaa
     2701 aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta gtcctgacac
     2761 tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc ctatctactc
     2821 atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca aacaagatat
     2881 tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg tatattttcc
     2941 ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg cgtttctaga
     3001 agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa aaagaaaaaa
     3061 gagcaagcat tagcacaaaa aatggaagag tttaccactt gttatgagga aatgaacgat
     3121 atttttgtga atcaagaaaa acgtttgacg gataaaaaca tggaaaaact cactcgtctt
     3181 tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag acaactatga gcgaaccaaa
     3241 aaagacgtgc aatatgtaaa agatacaaag atagaagaag cctgtagtga tctatttgga
     3301 gacacaaacc cttggtttgc cagtgaagaa gaaaaaaaag aaaaacaaca atctgtcaca
     3361 tatgaacgct atgaaatctt acttcaacaa gcaactaaca tgcaaaatcc gacaaaaaaa
     3421 gaaaccgcct taaactatct aaaaagtgtc aaggaatggt tagatcagca acaacaaaat
     3481 tgaatttgat ttaaaatcat gttaaactga atgtgcaaat gatcaaacat tagatacacg
     3541 aaagccccag agaggtcgga ttctctgggg ctttttttca ggttgatgtc gcctttattg
     3601 attttacttc ttgtttcggt tattcaacca ataactgaat aaagagacac aaactcctac
     3661 aagaaggggg caaatgaaca gttcaaacat tagatacacc tccttccagt cgcaagactg
     3721 tcggataggc gacaagttat tatatcttaa aaaattaaat ttgtgtggtg tttttaagta
     3781 tcacttgttt tagggttgtt agggcttgcc ctgaccgcct gtaaggcgct tctcgactag
     3841 attaagggaa attaatcaag aattgtctta gaacggctta gacaagcttt cagagtctca
     3901 cacctcctct ttgaacaacg gttttagcaa aggagccgga ggcgactgcg cagtataata
     3961 tgagagcata gcgatcacac ctttttagcg accgtaggga gcaagttttc cagtttagaa
     4021 aaattcgcga acctttttgg aggaaaaagt aggagttttt taggcgggaa attcaatgtg
     4081 ctcttaagat atactgttag atatactgtt agatatactg ttaataataa ctgttaggga
     4141 ttagcaacct tatgggagag taagtattga cataggtagt gggggataaa ttgtcacact
     4201 atgggggata aattgtcaca ctatggggga taaattgtca cactatgggg gataaattgt
     4261 cacactatgg gggataaatt gtgttttaca tcctcttatg tctatgataa cataatttat
     4321 tgaggtgatt tttattgagt aacgatatag ttcgatataa taatggatta aattctgtcc
     4381 ctttaagaaa atttacccct atagatatgg atttattttg gggaatatgt tctaaaatga
     4441 aaagaaaagg tacaaaagaa gaaattttta catttaaaca aataaaagaa ttaattgatt
     4501 acaatataaa tgcacgtggc gaccgttttg ctaatgattt aataaaaatg tcaaaaaaaa
     4561 tcattacttt gtcttttatt tatgaagacg aaaaaacata tgaacaatta aatctgtttc
     4621 aaaaatttaa aatcgataaa gtgaatgaaa cattattaat aaaggtttct gaagaatttg
     4681 aatatatact taattcaatc ggaactaatt ttactcgttt cgaattagaa aatatgacaa
     4741 acttaaagag tagctacgtt aaagagttat atcgccaact tatggctcat aaagacagaa
     4801 gatctaaaaa aggtcactgg tttgtaaaaa tagaagattt tagagctcta ttagcaatac
     4861 cagaaagtta tcgcatgaga gatattgata aacaaatttt tgcccaagcg aaaaaagaat
     4921 ttttaaacgt taattcaact caccctgcta tttttaaaac ttttgaaatc aaaaaaatta
     4981 aagcctacaa aggaaataag ataggatctc tgcaaattta ttttgaagaa aatatacctt
     5041 caattacctt agataattgg ttagagaaat aatacctaac aatttaggag gcaattatca
     5101 tgcaacccaa agaaaccttt acaataaagg aattatctga attatttgaa ataagtaggc
     5161 aagcaatctc taaacatgtt gataaattag acccgtcctt attagctaaa aatgagcgtg
     5221 gttataaggt tgtattacgt tctggagtat tgcaacttgc tagcaactta ggtaaccaaa
     5281 ggttactata taggttgcat gagtatgaac aagaaaacga taaaaacgtt tatatatcaa
     5341 gcaaaaacgt gattgtaaac gagttgctag ataagttgcg tgaagaaaat gaatttttac
     5401 gagaacaact ttccctcaaa gagcaaaata attctaatat gcaaaaatta ctcgaccagc
     5461 aacaacagct caccctacag gcaaatcaac agatcgaaaa actgcaagaa cagttacaat
     5521 tagcttatac ggaagaaact ccagaagatg aatcaactac tattctttct gaagaaacag
     5581 aatcgataga aaaacacaca aaaaagaaga agcaaaaatg gtggcaactt tggaaataac
     5641 tggagggaac attcatgaca agaaaaaatc ttctattcgg ttctatttta ctaggaattc
     5701 taggcttcag tccgatgtta ttgtcagtaa tcaataaagg gaataaacat ggtgaattga
     5761 aattgaatcc aaattattac aacagtccta acgaagaatt gtttgtaaaa gagacggata
     5821 aataataaaa aagactgctc ttccgagcag ccagttaaaa aataatgagt gtttattctt
     5881 gaaaaagcac gcgcttatga agcgctacca aaaatttacc atattttaca aaaaataaca
     5941 agtcttaaaa aaatgacaga taaaaatgca caaagaatta gatatagata accgcgaaaa
     6001 atcaagcttt agaaagttgc ttttagctag accaattaaa aatcacaaag tgagcgtctc
     6061 tatccattgg tagaggggct acttggcata gtcgacatta tgtcaactat gcaattaaat
     6121 aactaactac ttcatattag cttaataaaa taatcaaaat aactaggggg aaaaacattg
     6181 tataaaaaaa tttttcattt tttaaataag aatagtaaat ataaaaataa tttaagatat
     6241 ctattactat taatccagat agagcaaacc aagtaaatat tccatgtgta aagctaaaaa
     6301 aattcatttg aatcaattca aataaaccta atatagtaac aaat
//
LOCUS       DACDOA010000127         6239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDOA010000127 DACDOA010000000
VERSION     DACDOA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.128"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(451..936)
                     /locus_tag="IWI39_002516"
     CDS             complement(451..936)
                     /locus_tag="IWI39_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209553.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1075..1290)
                     /locus_tag="IWI39_002517"
     CDS             complement(1075..1290)
                     /locus_tag="IWI39_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9209554.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1514..2314
                     /locus_tag="IWI39_002518"
     CDS             1514..2314
                     /locus_tag="IWI39_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9209555.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2333..4201
                     /locus_tag="IWI39_002519"
     CDS             2333..4201
                     /locus_tag="IWI39_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9209556.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4194..4685
                     /locus_tag="IWI39_002520"
     CDS             4194..4685
                     /locus_tag="IWI39_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9209557.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4673..5227
                     /gene="srlA"
                     /locus_tag="IWI39_002521"
     CDS             4673..5227
                     /gene="srlA"
                     /locus_tag="IWI39_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9209558.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5245..>6239
                     /locus_tag="IWI39_002522"
     CDS             5245..>6239
                     /locus_tag="IWI39_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9209559.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2205 a          987 c         1237 g         1810 t
ORIGIN      
        1 atcatccttt caataagtat tttttattta tccctattta cgtaataact ctattttgaa
       61 aaaaatgcaa gtataatggt tagctttgtg aatatttcat ctaatacgat acaaaggcta
      121 tctttctaat ttgaaaaagg gatcattaaa ataataagaa gaaaatatgt caaaaggtaa
      181 aaaagtaaat tcttctatcc aaatatagaa aaattgaatc tagaaaaccg cccgacaata
      241 aatttcatta actcagttga ccgaaaatga agtagatacc aaaaatttat caagagagca
      301 agtagagaag tgagtatatt cataagagaa atagctgtaa cgaattatta ggttaatgca
      361 aatgcttatt tatactccgt ataactttct cagaaaaaat gataccaata atgacttgct
      421 agtactgtaa atccgtataa tatcatctaa tcaaaatggt gttccatttg catctgtcat
      481 cgtaatcgat ccttcgggtg acactcgata aaaaccaacg cttccagatc ctccttggga
      541 acggatttgt ttgctggaag ctgtgaacaa ataatctcga tcaatttttt gataaaagct
      601 aataacaata tcgtcacttt gtaattcgga acggtgggca ataagaaata gaagtgcttt
      661 gtcagagttt tctaatggaa gagaaggtac agtgctttct tgcatttcag atgttgaaga
      721 aatgctaaac gactgaatac ttgattggct atgttgcgaa tctacattac cagtagaagt
      781 attagtaatg gaagtagtag aagtactttt agttgaacca acctcagttg ttgaaatggc
      841 tgctgaagat tctttaacag aattctttgt tttatatcca caggcagaaa aaagtattac
      901 agttcccaac aagcctgcgt aaatcccttt atgcatgata gctcctccta aaatagatta
      961 tctttattca gtatatcact taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt
     1021 cccaataaag ggaaggttca tgaagataat aactttttta ataattatag ctttctaata
     1081 tctttcatca acttttctag taatcattgg gaaaaatttc aagcagtaga tcgcacaaat
     1141 acctacgacg atataaatga tcttcgcaaa aatagttgtt gaaccaccag caatcatagc
     1201 tactaaatca agttcgaaca agcctaccaa cagccagttc aaccctccca caatcaataa
     1261 agttaatgca atagcatcta aagttttcat tagaattctc ctttcttaaa tgataaaaca
     1321 attatatatc aaaaagagaa acattgctta tataacgctc aggaatttta gcaatctatg
     1381 caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc tgaagctgta
     1441 aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag aaaaaagaag
     1501 gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa ttgtaactgg
     1561 aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt
     1621 agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat ctttaaaagt
     1681 cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga aacatttcaa
     1741 gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc taatagatag
     1801 aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga tgatcgctgt
     1861 caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg tgcaaaaggg
     1921 atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag aaggacagag
     1981 ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg caaaagagtt
     2041 aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag aaaccggttt
     2101 gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg tcgaacagct
     2161 gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta agctgcaaga
     2221 agtggcggat ctagtctgct attatctttc agaaagatca agttatatca ctggagtcac
     2281 gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc acgtgacgct
     2341 agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag tttcaatcca
     2401 ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata ttggggagtt
     2461 gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga
     2521 aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa cagccagtga
     2581 tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg aaacagagga
     2641 tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa
     2701 ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta caggcacacc
     2761 aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt tattttattt
     2821 tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa aacaagcaat
     2881 caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt taacgaaagt
     2941 tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc ccatcaatcg
     3001 ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc agttggaaga
     3061 aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc ctttgaacat
     3121 catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct tatatttcga
     3181 ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt ccttcttata
     3241 tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc gttatcgatt
     3301 gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc aaatcgctag
     3361 cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag tagagatcag
     3421 ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt tttcctctaa
     3481 aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc aaagacaaat
     3541 caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac aatatgaaaa
     3601 agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga
     3661 agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa gggcacaatg
     3721 gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt tctatcaaat
     3781 atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc ctttgatcag
     3841 aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat atcagcaaag
     3901 cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat ctcaacacat
     3961 tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag gaaatggagt
     4021 tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg aattatttga
     4081 gctatacgat atcctatttg aactcataaa aatgggaata gatgagttta ttgaattagc
     4141 accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac ttcatgaatg
     4201 atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca gttatcagtt
     4261 ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag
     4321 cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta gctatagacg
     4381 atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc
     4441 attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac catccctcag
     4501 ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa gtatacttac
     4561 gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt gctatgctca
     4621 ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa cgatggatat
     4681 cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag aaacattcat
     4741 aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa tgaatacgct
     4801 gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag cgaaaaatcc
     4861 gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa atcctatgtc
     4921 atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg ctgctcaagc
     4981 tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag aattgtttgt
     5041 atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg agctggctat
     5101 ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa ctgattttac
     5161 tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag agctttcagt
     5221 tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag aggaaacggc
     5281 gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt catctatgtc
     5341 acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac aggtatggag
     5401 tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac
     5461 tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac catcaatatc
     5521 gtttccacag gaaaaagtgg cccattagca caatatatca ctgaagatat ttatgtttca
     5581 gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa agaaacagta
     5641 aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa
     5701 aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa agtagttgct
     5761 acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt gatccctttt
     5821 atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg taatgtattt
     5881 gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat tggctttatt
     5941 tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag ccaagtgatt
     6001 ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt agcattaccg
     6061 gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg
     6121 gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta ttcaagattt
     6181 cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACDOA010000128         6214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDOA010000128 DACDOA010000000
VERSION     DACDOA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.129"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..604)
                     /locus_tag="IWI39_002523"
     CDS             complement(74..604)
                     /locus_tag="IWI39_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9209560.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(786..1442)
                     /locus_tag="IWI39_002524"
     CDS             complement(786..1442)
                     /locus_tag="IWI39_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9209561.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1569..2042)
                     /gene="dfrF"
                     /locus_tag="IWI39_002525"
     CDS             complement(1569..2042)
                     /gene="dfrF"
                     /locus_tag="IWI39_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP9209562.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2437..3828)
                     /gene="amt"
                     /locus_tag="IWI39_002526"
     CDS             complement(2437..3828)
                     /gene="amt"
                     /locus_tag="IWI39_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9209563.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4169..4411)
                     /locus_tag="IWI39_002527"
     CDS             complement(4169..4411)
                     /locus_tag="IWI39_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9209564.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4443..5000)
                     /locus_tag="IWI39_002528"
     CDS             complement(4443..5000)
                     /locus_tag="IWI39_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9209565.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5013..5201)
                     /locus_tag="IWI39_002529"
     CDS             complement(5013..5201)
                     /locus_tag="IWI39_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9209566.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="IWI39_002530"
     CDS             complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="IWI39_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9209567.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1876 a         1292 c          905 g         2141 t
ORIGIN      
        1 aaatcttaag gattttcccg ccaacgtggt cggatcgctc cgtcggttta acagcttttc
       61 tgtttctctc tagttatcta cgcatataaa tcaaatcggt tattccgtta taagtctgtg
      121 ttttacaaag ccttaacttt atttccctgt ctatgttgcc caaaagacga accccatttc
      181 ctaacagagt aggaataaca gaaatgtaat atttatcaat catatttttg cttaccaact
      241 gctgaacaag atttgctccg ccacaaatcc aaatatcctt tcctgcttct tgttttagcc
      301 gttccaataa taaaacagga ttttcacttg taaaatgaat ttgttgggaa gaattgcact
      361 ctttatgtgt taatacataa gttgtaaatt tattatatac ccattcttgc ggagaaagtt
      421 cagtaacaac ctgatgagaa gtattccacc ccattaagac agtatctata tctttggtaa
      481 attctgtata agtgtcgatg ttttcattgt catttccatg accatttagc caagctactc
      541 caccattacc gtccgcaata tatccgtcaa ggctcatggc aataaataaa actacttttc
      601 tcattttttc tcttttataa atacaaacag gaatttattt ctctttttta ctcataaggg
      661 aacaactgat tgctacaaat aaacccatta aaatccatgg aaatgctgct cttataaaat
      721 cactaaccat actttattcc tccttaaatt ccaatttcct tagttctaca aactgaaatt
      781 tttgcctatg aatgtatctt gttctttata cgcaaacagg cttctgtgtg cataatccaa
      841 tgtcttgaaa acggcatttg aattagtcca caaatatctt tattacacca ataatcaatc
      901 agccaaattg ctttttcatt gtcgtttact acattcaacg attctaagta tccttttctt
      961 tctttcggta tttttctttt caattcatca taagataacc gttcaagcat tttctcagtg
     1021 ctttcccata cttcgaaaat atataaatat aattcttcca gattaagctg ttttgaaaag
     1081 tctgcaatct gctgtttcat gagttcattt cctgttgtaa tgataggtga attgatacgc
     1141 tcttgataat tgcctgcaaa aaatacttgt tcatcttcat ttatcactgt atgcacaact
     1201 atatcttcaa tgcggaaaat atgccaaata gaataagcaa tcgtcttact atgataacca
     1261 tcagcgttta tgaaggggat tgagttaaaa tcttctctgc ataattcttc tttgaaagat
     1321 actaaggttt gtattaactg acttcgtaaa gtaagtaaag tatcaatacc cctcttataa
     1381 gtatctttct tttttatttg tgcctgcatt gttttattaa gttcagacca ttccttattc
     1441 atattcaatc ctctctcaaa ttctgatttt atcgccccat acaaactgga atttgtctta
     1501 caacaattta tatatgcata aaaagcaata tatttatgaa aacaccctaa atccaaaatt
     1561 tactcaattc attttttcct tacataaaat gttctcgtat atttcacttc ctcaccaagt
     1621 gtttccccta tcaaaacttc aaaatcattg atatcaaatt ctggaaagaa tgtatctcca
     1681 tcttcaatat ttaaatctac ttctgtgata tacattttat ctactatttg caaagcttcc
     1741 ttaaatagtc catatccacc agatatgtat acatctcgtc ctttagccaa taataatgca
     1801 tcttctaatg atttaactga aactaaatta tctccttgat actctgttgt ggtggaaaca
     1861 acaatattca ttctattagg caacggatga ccgatttctt cataagactt tcgccccata
     1921 ataaccacat tacccgttgt taactctcta aattgctttt gttctccctt tattttccat
     1981 ggtatattac cattcttgcc tataacatta ttctttgacc tcgcaacaat caaacctatc
     2041 atctatttca tcctccataa tatgatgtcg tacctgtgat tccatcaagt gcaccacatc
     2101 ttaacaaact caaaattatc tcgaactaac cagctcgaca aattcctttt ttgtcttcat
     2161 ctgcattata tcatattgtt ataagcattg ccatttcttt tctgcttttg aaaagttact
     2221 tatcagccta tgttttgtaa agggtgatat tcagcatttc tcacgcaaca agcccttgac
     2281 aaaccataga ctaataagtt ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca
     2341 cggcaacaaa aaagagatac caacacactt atgtagtatc tcttagtcta gtagcaatat
     2401 tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa tatctttttg tttgggaaac
     2461 acctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
     2521 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     2581 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaagaa
     2641 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     2701 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     2761 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     2821 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     2881 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     2941 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     3001 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     3061 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     3121 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     3181 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     3241 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     3301 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     3361 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     3421 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     3481 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     3541 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     3601 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     3661 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
     3721 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
     3781 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
     3841 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
     3901 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
     3961 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
     4021 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
     4081 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
     4141 tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta
     4201 atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc
     4261 cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg
     4321 cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac
     4381 cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact
     4441 tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca
     4501 gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt
     4561 actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca
     4621 acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc
     4681 aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct
     4741 agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct
     4801 gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca
     4861 tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat
     4921 tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta
     4981 ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt
     5041 cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac
     5101 aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca
     5161 aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc
     5221 actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact
     5281 gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc
     5341 atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg
     5401 atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct
     5461 tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca
     5521 tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag
     5581 atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag
     5641 taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca
     5701 ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt
     5761 atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac
     5821 tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc
     5881 taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat
     5941 tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat attattcaag
     6001 attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta tttattaaat
     6061 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
     6121 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
     6181 aaatgaggta tgtcctatga tgaaaagatt gtac
//
LOCUS       DACDOA010000129         6142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDOA010000129 DACDOA010000000
VERSION     DACDOA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.130"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..1584
                     /locus_tag="IWI39_002531"
     CDS             133..1584
                     /locus_tag="IWI39_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9209568.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1933..2604
                     /locus_tag="IWI39_002532"
     CDS             1933..2604
                     /locus_tag="IWI39_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9209569.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            3152..4591
                     /locus_tag="IWI39_002533"
                     /pseudo
     CDS             3152..4591
                     /locus_tag="IWI39_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4936..5103
                     /locus_tag="IWI39_002534"
     CDS             4936..5103
                     /locus_tag="IWI39_002534"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209570.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            5362..5577
                     /locus_tag="IWI39_002535"
     CDS             5362..5577
                     /locus_tag="IWI39_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209571.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5765..6052)
                     /locus_tag="IWI39_002536"
                     /pseudo
     CDS             complement(5765..6052)
                     /locus_tag="IWI39_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2058 a          954 c         1233 g         1897 t
ORIGIN      
        1 ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc
       61 aattcattga aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga
      121 gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa
      181 ctctttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca
      241 aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa
      301 cttttgacaa ctttatcaag tgaaagtagt acgacttcaa atgctgagtc ttcaacaact
      361 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc gtcaagtgaa
      421 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      481 tcaagtgccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      541 acaagaccaa accatataga gtcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      601 aaaaattttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      661 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      721 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
      781 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
      841 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
      901 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
      961 tatgaggaaa gttttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
     1021 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
     1081 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
     1141 acctatgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1201 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1261 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1321 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1381 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1441 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1501 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1561 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1621 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1681 gagtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1741 gctcatattt tgagcaggtt ttactagatg aaaacaattt atctatcttg aggatttcgt
     1801 gtacgtatcg ttcagtcttg tattataaat tagtcataaa atcagactaa gaaaaattga
     1861 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     1921 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     1981 aatggatata aaaaatatcg ttacctatgt gactgtggga atgaaacgat tgtatatcga
     2041 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     2101 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     2161 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     2221 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     2281 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     2341 aactgttcat ggaaaacaaa atgggtttgt caatgtgagt gtggcagtat gtgtgagata
     2401 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     2461 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     2521 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatggattgc gtatataact
     2581 ttaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca gctcaagtta
     2641 gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga ggtaatcatt
     2701 aattattgtg gataataatt aacagtgata tataaacatc tataatatat tattcatact
     2761 attgacattt agtataatcc tatggaattg aatatcatag caatgtattt gaaaagcaaa
     2821 ttggaaaaca aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa gtagaatgga
     2881 aattttctta cgtctcacga gatagctcac actattaatc tgaagaacaa tgaagtcgac
     2941 ttttagaaat tgctagaaga aagtaaagcg aattatcact agttactata gagtaagttc
     3001 aaataaagca tctcaaactt acatggttac atgagaataa caaatcgaaa ttactcaagc
     3061 ttcctatggc tagagtcgcg agaagttgtt gggcagtaga cataggttat attgttctta
     3121 gctacacgat cattagaaag gaaatcctat tatgcatagt tatcaagaag atgcaaaagt
     3181 ccaaaaaaat cgctggtgga ttttgattgc tatatctatg tttaccttca tgtccacatt
     3241 ggatggcagt attgtcaata ttgcactacc taccatctca gatgatatgg gcataccaat
     3301 gaatcaagcg gaatgggtag tgtccgttta tctgatgatt gtctgttctt gtttgttgct
     3361 gtttggtaaa attggcgata gctgtgggaa aatcaaagta tttcggattg gtacgctgat
     3421 ctttgtcttg ggctcgctgt tgtgtgggtt caatcattcg ctaggtattt aattgttcgc
     3481 acgggtggta caagccattg gtgctagtat gactatggct acaaatgccg gaatcatcac
     3541 ggaagtcttt cccatacatg aacgtggtcg ggcattggga gcaatcggtg cgcttgtttc
     3601 attaggctcg attgctggac caggtcttgg cggcttgatt ttgtcccaat tttcttgggc
     3661 gtacattttt tggatcaatg taccggtggg aatcgtaacg atgttgattg ctgaaaaatt
     3721 cttaccaaaa gatatcacca tgagcggcca tacaatcgat ggagggggct ttgctgccat
     3781 tgcggctacg attatcagcc tttttggaac aatctttttg gggcaagaaa gtggcttttt
     3841 ccatccagtc ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca gctttttaaa
     3901 agttgaaaag aaagcagcct ctccattgat ttctttcggt atttttaaga acaagatttt
     3961 taccatgagc ttgattacag ccttgttgat tttttcttcc aacttctttg ttaatgtagt
     4021 gattcctttt tatctgcaaa atgctcgcgg gttgagtcct agctatgccg gactgttgat
     4081 gatggtcttt cccttgatga tggtcgtcgg ggcgccttta agcggatacc tgaccgaccg
     4141 ggttggcccg gagatttttg tattagtagg cttgggttta ttgtcggtaa cccaagtcat
     4201 gtatatgttt ttgtcagtgg actctccttt gtggctgtac attgtcgcta ctgccatcat
     4261 gggtgccggc aatgccttgt tccagtcccc aaataatacg atggttatga gttctgtcac
     4321 tatcgaaaat ttgggactgg caggctccat gaattccttt gcccgaaatt tggggatggt
     4381 catcgggatt tcgctttcta cgacggtctt atatcaagcg atgtctgcta agatgggcca
     4441 acgagtaact aactatatcg cagatcggcc ggatgtcttt ttatatggaa tgaagattac
     4501 ctttttaggc agctttgttt tgtgttttgt tgcctttttc ctgactatct ggcgtatcaa
     4561 aaaacaagga ggtattcaga aaaatgtctg aaaaaccatt gttgaagaat ttttggaggc
     4621 ctgcgtatgg gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctgaaaac
     4681 cgaaaaactc aagtattatc ttcaaaatac tagtactcaa ttagatgctc gatttgatta
     4741 caagaagact gcgaaagcca ctggcaaccg caagctaggt atccaccgat ggactacgtt
     4801 ttagaggcaa ctggttatga atacggagct aacgctctca tctgcataag aatgcaacac
     4861 ccagattatg taaagatttt cgatacttct gtcaaaaaaa tcatcaagat agataaatga
     4921 tttccgtcta gtcaaattgg ttcagaatgt ggacacacag atggtaagaa gtcattcaca
     4981 attcgagaat ggacttgtcc tatttgccgt acacatcatg atcgaaacgt caacgttagt
     5041 atcaatattt tgatcaaggg ctatgtatac aagcattgcc ttagaaaaca tcaagaacct
     5101 taggaacaac ggggatagat tggttaataa gagatatcgc tgttagtaaa gacataggct
     5161 tgcaagtata ctcttttatc aagaattccg tggctttagt catgataggt ttaatactat
     5221 gaaaattttc tttagcattt ttgatttagg ttccttttct aattataata aaatgaccac
     5281 tacagtaacc tactgaaata tataaacagc cactgtagta ccattttcaa agtagtctat
     5341 gagaggagaa tcttcattct tatgaacgct ggaattgcgg atatgaattt aataaaaaaa
     5401 acccttaatg attttacttc caattcaata tcaaaaggta ctggtatcaa tttaagcaca
     5461 ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt agatgctata
     5521 aaaataacgg aatttgctat gaaaaatgga aaagctgaga ttgaaatatg gagataatta
     5581 tcgtctagtg aactacttgc cacttagctg aatttaacga tccttagtgc ttgaagtggg
     5641 agattcttgg gaatagagca tacttgcaag cttatgtctt tattaacagc gatatctctt
     5701 attaaccaag ctatccccgt agttctaagg tttttacttt tatctaagct aatgatcgta
     5761 ttcaaaggaa atttttgacc cttagaaagt ctcattttat cagacatgag cttaggcatt
     5821 gctgaatttt gttaattcgg ttttaggttg tgctttgagc ataatatgaa agtagtcttt
     5881 atcacgattt cactcaacta aggtaatgtg atgtgattct gatattcttt caaagatagt
     5941 tttttgcata agctaacatt acatcatcaa tgagatgttt acaatgtttc actactaaaa
     6001 taaggtggta acaaagaaga aataatgaat gattatttgt atctaattcc atggtatatc
     6061 aaccatttct tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta
     6121 attgtcggtt ttcgagttac cg
//
LOCUS       DACDOA010000130         6065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDOA010000130 DACDOA010000000
VERSION     DACDOA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.131"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IWI39_002537"
     CDS             832..1566
                     /locus_tag="IWI39_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9209572.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IWI39_002538"
     CDS             1582..2751
                     /locus_tag="IWI39_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209573.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IWI39_002539"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IWI39_002539"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9209574.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IWI39_002540"
     CDS             3841..4767
                     /locus_tag="IWI39_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9209575.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IWI39_002541"
     CDS             4786..6036
                     /locus_tag="IWI39_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209576.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DACDOA010000131         5959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDOA010000131 DACDOA010000000
VERSION     DACDOA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.132"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            136..615
                     /locus_tag="IWI39_002542"
     CDS             136..615
                     /locus_tag="IWI39_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9209577.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGMIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            834..1514
                     /locus_tag="IWI39_002543"
     CDS             834..1514
                     /locus_tag="IWI39_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9209578.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            1522..2853
                     /locus_tag="IWI39_002544"
     CDS             1522..2853
                     /locus_tag="IWI39_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9209579.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            2868..3830
                     /locus_tag="IWI39_002545"
     CDS             2868..3830
                     /locus_tag="IWI39_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9209580.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            3941..4279
                     /locus_tag="IWI39_002546"
     CDS             3941..4279
                     /locus_tag="IWI39_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9209581.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            4358..5065
                     /locus_tag="IWI39_002547"
     CDS             4358..5065
                     /locus_tag="IWI39_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9209582.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(5168..>5959)
                     /locus_tag="IWI39_002548"
     CDS             complement(5168..>5959)
                     /locus_tag="IWI39_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9209583.1"
                     /translation="ELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFP
                     NQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVV
                     FFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQL
                     IQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQ
                     HEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         1970 a         1024 c         1294 g         1671 t
ORIGIN      
        1 aaaaaataaa caaaaagagt aagatgaacc tctctataga gtgctcgtct tgctcttttt
       61 ttgcgaaaaa caaataattt ttgaatacag catccctcgc tttataataa acgatagaat
      121 aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac gttatttaga agaacaaaag
      181 atcagctatc aaccacttga ttttagtcat gtggtagaag aatctttcac agaagaacta
      241 caaaaaagag ggattgatcc agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa
      301 actgggatga tcatcgcatt gattcctttg aacgatcact tagattacaa aaaaacgaga
      361 caagtgacga aagatcgtaa agttggtttt cctggaatgg attttgtatt aatgcataca
      421 ggatatcccc acggagcaaa cacccctatt ggaataaaac tcgcacatcc tgattattgt
      481 tttttagcag acagttcttt aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc
      541 gggagaagcc ttcggataaa agtagccgat ttagaaaaga tcgtccatcc tatttacgct
      601 gatttaatta gttaagggac gagaagagaa gctaagcaga gcagagacct caaacttgag
      661 attatatctg cttggtactt ggtgcatcct gtattatctg ttacaattgc tttgtacata
      721 ctgtactatc aatccaatga aaatatattt agcagctata ttttttcatt gatgtgcaag
      781 tgatgaaatc gtttttaata aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa
      841 ttacgtatca tggacattcc tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga
      901 ttgacccgtt tattacagga aatccattga cagacttaac tgcagaagaa gtaaagacag
      961 attggttgct gattactcat ggtcatagtg accacatagg agatatgatt cctattgcaa
     1021 aagaaaatca agccacaatt atcagtattg ttgagattgc tgattatgcg aacagcagag
     1081 gagtagattc attcggtatg aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa
     1141 aattcgtaca tgcacagcat agttcaagct atgaagtaga cggtatcgtt cagtatatgg
     1201 gcgagcctag cggaattatt attcaagcag aaggaaaaac gatttatcat gcaggggata
     1261 ctgcttattt cagtgatcta ggattgcttg cagaagaatt cgatattgat gtcgctttct
     1321 tgccgattgg cgataactat acgatggggc cagaagatgc agcaaaggct gcgcaaacaa
     1381 ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt ccctgtcatt gaacaagatc
     1441 ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa aataatgagc gtctcggaaa
     1501 cgctggaagt gtaggcagaa aatggcgaca aaacatgatt tgattttaga atatattgaa
     1561 agtttacctg ttggcaatcg aatttctgta agaagcatcg caaaagaatt gaatgtcagt
     1621 gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg tcggactagt ttcaacaatt
     1681 caacgagtag ggaccatccg tatcgaacgc aaattaaaaa aacatattga acgtttgaca
     1741 tttggcgagg tcgtcaggat cattgaaggg gacgttttag gcggggcggc aggattagac
     1801 aaggtcttga ataaattcgt catcggcgcg atgactaaac atgccatgga acgctatatt
     1861 acgccaggat cactaatgat cgtcggcaac cgggcggaag tccaaaaatt agcattggaa
     1921 gatggcgccg cagtattgat cacaggagga ttcaatacga ctgctgagat tgcaaagcta
     1981 gctgatgatc ttgaaatgcc gatcttacgc acctcttatg atacatttac agtcgcaacc
     2041 atgatcaacc gagcgttaag cgaccaattg atcaaaaaag atatcatgct tgtcagtgat
     2101 atctatatgc cagctgaaaa gactcattat ttgcagcaga cagatacgat ttctgattat
     2161 cagcgtttgt cagaagaaac gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt
     2221 ttgatgggga ttgttacggc aaaagatgtt ttagggaaat cgccaaacca gatcatcgat
     2281 cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa tgagtattgc ctctgttagc
     2341 catcaaatga tctgggatgg gctagaagtc atgccggtcg tttccgatga cttgacattg
     2401 gaaggcttga ttacccggca agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg
     2461 cagattgcag atacgatttc ggatcagata tctggaaata tccatacgat tgatactgac
     2521 cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg tagtagcacc acaaatggtc
     2581 aatggcgttg gaaccatttc ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa
     2641 aaaacaatgg tgatgaacca aaaacgaaat atcttgattg aacaagtgaa cctacattat
     2701 ttgcggctga tccaattgga aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc
     2761 agacgttccg ctaaactgga tatcgaagtg tttattgaaa acaccatcgt ttccaaagca
     2821 atcgttgttt gtcaagtaat ggaacgttct tagaaagcag gagtgaaatg acagtacagc
     2881 aagaaatagt agatacaata aaaaaatacg ataaaatcat tatccacagg catttgcgcc
     2941 ctgatcctga tgcattagga tcacaagtag gattagccga actattacgg gaaagtttcc
     3001 cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa
     3061 tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt catcgtgact gatacagcta
     3121 attcacccag aatcagtgat caaagatatc agttggcaga aaaactgatc aaaatcgacc
     3181 atcatcccaa tgatgaccct tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct
     3241 gtagtgagat gattgcagaa tttgctttga tgtttcctga atctttaaag atgaacgtta
     3301 aggctgcacg gttactttat gcaggaatcg tcggagatac agggcgcttt ttgtatccct
     3361 ccacgacgtc ccgtacatta gagattgctt cgatattgag aaattatcca tttgatgcaa
     3421 gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt cgctaaactt tccggttatc
     3481 tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa
     3541 ttcttgataa atatggaatc gatgattcag agacagcttc gatcgtttca cttcctggag
     3601 tgattgacga agtgctggct tgggggattt ttgttcagca gccttcaggg tattaccgcg
     3661 tacgtttacg ttctaagggg ccgatcatca atgaattggc taaacgccat catggaggag
     3721 gccatccatt agcaagtggt gcgaatgcaa aagatttagc agaagtagag gaaatctata
     3781 aagagatcca agatatatgc caatcgtata agagcagaaa aacagaatga tacttcgttc
     3841 gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt ttcaagatct tctttttccg
     3901 ttacaatagt gctcactaaa gattttaaag gagaaagaag atgacattac caaattgccc
     3961 agaatgtggc tcagagtaca catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg
     4021 gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa gacggattag tggtattaga
     4081 tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca gtgattaaag acttgaaagt
     4141 aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga
     4201 aggcgatcat aatatcgatt gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga
     4261 atttgtgaaa aagaattaaa gaatattcaa ggtcttttca cattcttttc ctactactaa
     4321 tgataaaata aagtaaacga aaaggagtga aacgaaaatg tatggttatg gaatgtatgg
     4381 ttttttagat ccgacatttc ttttggtcat tcttggatta gttatttccg gaattgcgtc
     4441 agcgtacgtc aatagtactt tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac
     4501 tggtacacaa gcagcacaat atatattaca aagtcaacag ataaataatg tcggtgttca
     4561 acaaattgcc ggagatctca cagataacta caattcaggt aataagatgt tgagtttgtc
     4621 tcaagcaact gcacagtcta cttcagttgc tgcgataggt gtagcagctc atgaatgcgg
     4681 acatgcggta caagatgctt cgggttatgt cccattgaag attcgtgcag cacttgttcc
     4741 agcagctaac tttggttcga tgatttcgtt ccccttgatt atggtaggtg tattgcttag
     4801 ctggaactct acgttaatca atatcgggat tttcgctttt tctttagcat tacttttcca
     4861 attggtgaca ttaccagtcg aatttaacgc gtctcggcga gcaatccaaa ttttgagtga
     4921 aggcggacta ctgacggaag aagaagtacc gatggcaaga cacgtattat ttgcagcagc
     4981 actgacttat gttgcagcag cgctttcgac attcctgcag ctgttgcgct tgatcttatt
     5041 gttcggcggc aaccgaagag attaaaataa agaagttgtg aaatcagcgc tttgatctga
     5101 gctaataccg agagttagct gattgaatac catttatttg gaataagagg atgtgacaag
     5161 aattttgtca catcctcttt gttttgttct cataaccttt tttcatatac agttcatgaa
     5221 ccgcttttat taacttgcca aataacactg tattgtgcgt tgtatccaga tagaaagtct
     5281 ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat caaatcagca tgttctgaat
     5341 atgcggtaat tttaacagca tctttgcgaa ataactgctg caaatggctt tgaagatgat
     5401 aatttcctgt aaactgtttg gttatctgca aatggatgag taactgctct ttcgtttctg
     5461 tttgtagtaa gtgttggatc aattgactaa ggtattgact gatttggttt ctttccccat
     5521 tttttgaaaa aattgtatcg attttttctc ttaatttctt ttcctgtata aaagaactag
     5581 aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa actactgctg gattcaccca
     5641 acagttcgaa aaaaacaact gccaacacaa gaaaataaaa ataaattggt attttatttt
     5701 ctaaatggtt gagaggaaac tgttgagtta agtaagtcat caattgaccg acttgtcgac
     5761 aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga
     5821 tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta ggctctctct gtttctgtct
     5881 cttcacaagg gcgactcact gaaaaagaag taaagtattc tgaataatca tgtgtatgct
     5941 gcaataagtc catcaattc
//
LOCUS       DACDOA010000132         5958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDOA010000132 DACDOA010000000
VERSION     DACDOA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.133"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            166..384
                     /locus_tag="IWI39_002549"
     CDS             166..384
                     /locus_tag="IWI39_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209584.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            371..3049
                     /locus_tag="IWI39_002550"
     CDS             371..3049
                     /locus_tag="IWI39_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209585.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3040..3237
                     /locus_tag="IWI39_002551"
     CDS             3040..3237
                     /locus_tag="IWI39_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209586.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3241..3852
                     /gene="mobC"
                     /locus_tag="IWI39_002552"
     CDS             3241..3852
                     /gene="mobC"
                     /locus_tag="IWI39_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP9209587.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            3837..5228
                     /locus_tag="IWI39_002553"
     CDS             3837..5228
                     /locus_tag="IWI39_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9209588.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5444..5758
                     /locus_tag="IWI39_002554"
     CDS             5444..5758
                     /locus_tag="IWI39_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209589.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            5751..>5958
                     /locus_tag="IWI39_002555"
     CDS             5751..>5958
                     /locus_tag="IWI39_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209590.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEV"
BASE COUNT         2269 a          833 c         1170 g         1686 t
ORIGIN      
        1 caaaaagacg attatatctt tgcttctcta aaaaataaag cgttagaact aattgaattg
       61 caaatcagcg aaggaataga taagcaataa gaatagaatt gagtcaaatg aaatggaggg
      121 gtatctccac ttcatttgag aactttgaat ataggagggg ttattttgat taaaaatcgt
      181 ttaatagatc aagtttggtg tctagatcaa aatacagata gtctcgaatt aatcgagact
      241 atttgcttca acactatagt tctcgattta agagctgaaa atgataattg tgtaactcat
      301 gtcataatta atcaaaaaat ggctcagaag ctctctgaga gcctcagaaa atgggctaag
      361 ggaggtaacg atgaaaataa ttgaaaaaaa gattgccaat aaaaaaataa attggttggt
      421 tgttaatttt cttgttctga tagctatgat tatttttaca agtaatattg ctcatgcgag
      481 tattttcaat tggtttgatg acacaaaaag tcaaacagag tttttgaata attctcttta
      541 tagaccttac ctaaaaaagt acaatggaga tttccttagt cagttcggga tttggctcgg
      601 atgggctgtg gtaaaaggca tgtttactgt gacagattcg attcaaaata tgattccaga
      661 tgtgctggat ttgttcaatt tcattgaaag tacagggcta aataatgttt atcaatcagc
      721 cgtaaataca attgtcattg gattaatgat cctttcattg atgtttgttg gttacaaaat
      781 gattactgga aaaggaacag tggatttaaa atcggttgga atgaacgtag tgatgtctat
      841 agcattaata cttttaatgc caaccatgat ttctagtggt attcaatttg ctaaaacatt
      901 ttataaggac tctactacga ttacaaattc agatgacgga gtcgcttggt cattgataaa
      961 acaaggagta acagatttag catatattaa taaaacagat cagtactcaa gcattgataa
     1021 aacggaggat cgaaataatt taacacgaaa aaattttcaa cagacggatc tcacacaagt
     1081 gttaacggat aaagtgattg ataaattgga aaaagaaaat ccaattgcgg acaatcttcg
     1141 ttatgaatta gttgaaaatt ctaataatga atttgttgct acaaagtttt ctgacaactt
     1201 cttatcaaca ttttctgata gcttgaaatc aggctattac cgataccaag caaatctatg
     1261 ggggatttca attgggttga cagcgttagc aatcgcctat gtgttcagcg cgtttgtgat
     1321 cattacagca atactagaac ttgcatttaa acgtgtgtta ggtgttcttg tttttgcaac
     1381 agatattgag acaggacagc gttcaaaagt tgttctatca gatattctac aatgttactt
     1441 aactgttggg tttcaaggat ttgggttatc aatgtttgct atgtttatca attttctgaa
     1501 ctcaggacaa ggtatctcaa caaatatttt tataaaaaca attgcttata tctgtgccgt
     1561 ttttgtttta atcaaaggca gtggaacagt catgagatac tttggtgttg atattggact
     1621 caaagaaggc tatgggcaac ttgcttctgc ttttggaatg ggcgcaatgc tgtttagaaa
     1681 aggatcaaat ggatttaatc gtgctaaagg aagcggagaa ggagaagatc gaaaaccaga
     1741 aaagaatttc ggcgaaactc tatcaaaaaa agctggcaaa actggaagag ctttgggata
     1801 tgcccatgaa cgtggattat ccggtttagc ttcagatgga gctacaatgg ctagtgaaag
     1861 agcaacaaaa ccatttaaga gtatgcgtga tatagctaat gataccaaga ataagtttaa
     1921 agaaggttta gatgatggta cagtgtccgc tattaataaa aatagcaaac ctatgttagc
     1981 taaaaataaa gaagatgaaa ctggaaaata tgctgattcc gtgccaacac gtctttcaga
     2041 tagaaaagat ggtgctaaag ttgaaaatgc cgatagaatt atgtctagtt ctgaacgtat
     2101 gcgtgaagca atgaaaaata atgctgaatc gaataataat cctgcaagcg caattcagca
     2161 aaaagtccaa caagatattg aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc
     2221 tgaagaatta attaatcaga agcgtcatga agcgaaatac acgcctgaag caatgaatcg
     2281 cgaggaaata atgttaagga aacgtgtaga agggcggaca ggcgctaata ttgacggaaa
     2341 agaagcgttg accaaagaaa gaattcaaga agctaaaaat tcaaatacag gattagaaaa
     2401 attagtcaaa gaaaatccac aaaattctac aagtagtcat ggtggccgat cagtagatgt
     2461 ccgagaaaat attcaaggtt cactgaatgg tcatgttgga cggaatgttg atgtaagaga
     2521 gaatttacaa agttcttcaa atgctcaagg tactaagaca attgatgttc gagaaaatct
     2581 gcaaagtagc agtacaagtc aaccaaaaac agtagatgtt cgagaaaatg tacaaaattc
     2641 tacaagtagt caagggttta aatcagtgga tgtccgagag aatgtgcaag gtagcagtgc
     2701 aagtcaacca aaaacagtag atgtccgaga aaatgtacaa agttctacaa gtagtcaagg
     2761 attcaaaaca gtagatgttc gggagaacat tcaaaaagat agtggagaaa tgaaagaaaa
     2821 aacacaaaaa atcaatatcg ttgaagacag aaagtcagcg aaaaaatttg attcaaatca
     2881 tgaaacagtg attattgata gtgaaatccg tgaaaatgat aagggatcaa aatcaagacg
     2941 aagattcact tatgaagaca atgagttatt cagagataca cttaatgatc ctaatccact
     3001 tttcgataat ttattaagaa ataaaagggg gggattgtca tgcaattaat tctaaagatt
     3061 agtacgcatg atttcttaga acttgacgaa ttatgtgaat cagtaaagaa cgcaacaaat
     3121 gaaaaaatca cggtcactaa ttatattgaa ctgatgattc aagaagaatt aaagatgaaa
     3181 aatgaaatag agagcaattt caaatgttgc tctctagaaa aaaatgtgag gggataatca
     3241 atggatgata aacaaaaaat gtacgaaaaa aatcgcaaac atagtgaggc cttaaatttt
     3301 cgtgtcgata aaaagactta tcttgaactg acattgattg caaaacaaag gggaatttct
     3361 agagcagacg tttgccgtga gcggttaaag ggactcaaat atgaaccgcc gttgttagct
     3421 gaagatgata gggtagtttt aaccaaacag ttaaatcaaa ttggtaacaa tttgaatcaa
     3481 gcggtaaagg cattaaatat cattgctaaa tattttagag aagaatctga agataaaaaa
     3541 gaaaagagcc tacattttag ttttgatgct agccgttaca acatttttaa agatgaactt
     3601 gcgaatagca tagcaaaaaa gttgatgatt agttatccag atgacatttt tttggagtcc
     3661 aatcggagtg ccccctcttt ttttaaatta tcagaagtag aaaaagaata tttcgaatat
     3721 tggatagaaa agcaaactag accgggtacg aaagaagagt attcccaaaa agttgatcgg
     3781 aatttaattc actcaattca gattattgca agtttaaaag atgaggtgaa acaactttgg
     3841 gagctactat aacatcgaaa ggacgcaaga aaaatttatc ctcgctatta caatatgcag
     3901 ctaatccttc aaaaaaagaa aaatgtgttg cagtctcagg aataaattgt agtgatgata
     3961 gtgaggaagc actgcaagaa atgaatttag tacgtaccaa gatgaataaa tctaaagata
     4021 tagcacaagc gtttcatgtt attcattcgt ttgataagag tacttctaaa gagttaagta
     4081 ttgataaaat gcacgaaata gctgtcgaat ttgcagagaa agcttttcca aattcgcaaa
     4141 tcattgttgc aagtcacaat gataaagatc attttcatag tcatcttgtc attaataata
     4201 ttaacatgga aacatgtaaa aggttgcgta ttgaccctaa agatttagaa ctaatgcgaa
     4261 aaattaacga tcaagtcctc aatgaacatg gtcttgaatc agtgaataat gagtactatg
     4321 aaaacttaga aaagagaacc aatctttatt caaacgatcc ttctaagatg aaatatggaa
     4381 aaattaatta tcaagagttt gtgcaaaatg cgatcgaagc aacgttggaa aaatgcgaag
     4441 tgaattcttt tgaagatttt tcggatcatt tggcaaaaga atataacatt gaagtttatc
     4501 aattttcaaa aacaaataac aaacttggct atgttcttta caaacaaggt gtaaatttct
     4561 atgagcgtag aggtgagtta attactttag gaagagataa acaatctaga aaagaaaaaa
     4621 gtcagtatgt cgaacgtact tttagtgcaa gaaagttagg gaaaagatat tcatttgaat
     4681 ctgtttctga acaacttgaa aagaatttag aagaatcaca tataaaaaat ttttttaata
     4741 tggaaagaga gcaagttatg tcgctaatta gtggagtaaa ttacaaaact ggtgagttaa
     4801 atgtaccgtt tgtggaatta gattccatcg cacaaaagca atttagcgac cgtgaaggcg
     4861 atgaaaaatt cattaaatat gatgacttct tgcgagaggt agacaagcat tttgtaaata
     4921 caatgtcttt agaagatgat caagaataca cgattaaact tcatacggat gtgaatcctc
     4981 aaactttacg tagtgcgaaa agtaaaacaa tttctattaa aaagactgat cagaaagagt
     5041 tatttctacc ggcaaagggt tttgaaaatt gttctcatac accttctgct aaacagattc
     5101 actatcaaat cagtcgcata atgaatgacg atgtaaaaat gtatcgtgct aaaaaggatt
     5161 atgaattcac acaacaaatg attgaaaatc agcaaaaaag gcaacaaaat tttgaaatgg
     5221 aattctgaat gggcttgtta gtggactttc cagttttttg cctatgaaga gttgcttgga
     5281 aagaagcagt ttttaggaca tacgccagct acccatctac ttgccagtca atccgccccg
     5341 ctcacgctag gctactttct ggcaagtcag atggcgggta aatctctgag ggagcaagct
     5401 ccctacagac tccctcccaa aaaaagaaag gtggaagaaa aaaatgaact atccatattt
     5461 taaagtctcg gcttcagaag aaactaaaga aatattcaac aatttttaca accaaaacaa
     5521 aggaattttt ggttcaaagg caaatatgtt tcgtgttatg gtttcaaatt taccagtgtt
     5581 agctagtcca tcaaataata aatttaatga tcctgaaagc attaaatttg aacaaaaaat
     5641 ttcagaacta gaatcaatga taagtaatga agtaattgaa aagctggatg acattgatca
     5701 aaagttatcg tattctctaa aaaacaaata caaaacggag gagaaaaaag atgtttaaag
     5761 tttacttccc gtttcagtgg ctcgttaaag gaattctttt agtgctacta ggattgattc
     5821 tttttggaag ttatctaaca attaaagcca gacattttcc agaagaaaag ccaataaaaa
     5881 ttgtaacaaa agatttaaaa ataggtgaaa cttatcaggc agatggggtt gaactaacat
     5941 ttaaaaatat tgaagtga
//
LOCUS       DACDOA010000133         5803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDOA010000133 DACDOA010000000
VERSION     DACDOA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.134"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(48..710)
                     /gene="trmB"
                     /locus_tag="IWI39_002556"
     CDS             complement(48..710)
                     /gene="trmB"
                     /locus_tag="IWI39_002556"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9209591.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(780..1559)
                     /locus_tag="IWI39_002557"
     CDS             complement(780..1559)
                     /locus_tag="IWI39_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9209592.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1766..2977)
                     /locus_tag="IWI39_002558"
     CDS             complement(1766..2977)
                     /locus_tag="IWI39_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209593.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2970..3707)
                     /locus_tag="IWI39_002559"
     CDS             complement(2970..3707)
                     /locus_tag="IWI39_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209594.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3845..4279
                     /locus_tag="IWI39_002560"
     CDS             3845..4279
                     /locus_tag="IWI39_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9209595.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4272..4616
                     /locus_tag="IWI39_002561"
     CDS             4272..4616
                     /locus_tag="IWI39_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9209596.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            4796..>5803
                     /locus_tag="IWI39_002562"
     CDS             4796..>5803
                     /locus_tag="IWI39_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9209597.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         1937 a         1161 c          968 g         1737 t
ORIGIN      
        1 gatttattta tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc
       61 tttttggtct tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa
      121 aatttttctt catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac
      181 catacctgtt tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat
      241 ccttgattat ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca
      301 gcaagaaaag tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg
      361 gaaaaattca gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat
      421 ccatccacat gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca
      481 atcgaaacaa cactgacctg catttctact ccaatataat tgatttcagg atgggctttt
      541 gccattccag tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg
      601 ttttcaaaca attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc
      661 gggtgagccg ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc
      721 cattcctttc ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct
      781 cagccactga aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca
      841 cgtttgcaat atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt
      901 tcaagcattt cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac
      961 agtggcacat aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt
     1021 gaatcccaat cgactagata taaataattt ttacatacta accaattacg atggtttaca
     1081 tctccgtgta ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa
     1141 cgatggacaa tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat
     1201 ttttgcagca tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta
     1261 agagaatttg aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc
     1321 tcattaggag tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc
     1381 cccgttcgtt ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac
     1441 atcggagtcg tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt
     1501 tttcctgtgt cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc
     1561 atgcattctc ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa
     1621 cactacacgt caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct
     1681 tttctcaaaa aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc
     1741 atttttaccc tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat
     1801 atagtttaac aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct
     1861 gcccatctaa tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc
     1921 atgtgagtag atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa
     1981 cgagaaccat atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata
     2041 aaaataagaa accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc
     2101 cacctataat tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc
     2161 ttgcaaacaa gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat
     2221 ccaaatagcg tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga
     2281 taaattgata gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca
     2341 atcctttttc ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac
     2401 ctgctattcc tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa
     2461 aaaaccagcc taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc
     2521 tcttgatttg cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca
     2581 tcgaaaagga ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata
     2641 tcaaaaagat caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca
     2701 tttctttttc tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct
     2761 tacccacgaa taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg
     2821 gtggctgcag ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa
     2881 gacaaactaa aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt
     2941 gatgtcttgc taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca
     3001 atatagattt cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga
     3061 gaaccgtctg ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct
     3121 gcagtagcta aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc
     3181 atcaattcaa gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg
     3241 atatataaag aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc
     3301 tttgaaaaat tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac
     3361 ttttctgctc ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg
     3421 atatgttctc tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt
     3481 tgtttgcgat attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct
     3541 tttgaagctt ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc
     3601 agtccaatca accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca
     3661 gggagatgtc cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg
     3721 ccatttattt ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac
     3781 aatagcttta acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt
     3841 ttatatggaa aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat
     3901 atatgaagac gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac
     3961 attagttgtg cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg
     4021 agaggttttt tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat
     4081 gaaaggtctt aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc
     4141 acatatccat ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa
     4201 tcatcaagat agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca
     4261 ggtgtcctgc gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca
     4321 ctgtcggagg tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg
     4381 aagaattgga agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta
     4441 aaagcgcatt gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag
     4501 caatcaaaaa agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca
     4561 atgaacaagt agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat
     4621 gattattttc tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa
     4681 tgtgatgaaa ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg
     4741 atatggtata gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa
     4801 aaaaaaatca attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg
     4861 ttcaggcgat actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga
     4921 tttctatgat gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat
     4981 ttataaagta ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga
     5041 atacgacaaa caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg
     5101 atatacgaaa gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt
     5161 gaaagctcac gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc
     5221 agaagtagaa gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa
     5281 atcagcggat gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga
     5341 gacaaaagaa gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga
     5401 agtaaaagaa gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac
     5461 aaatcctact tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa
     5521 aggcaatgac atggataaat acaaagatca actaaaagat atcgctactg aaactaaact
     5581 aagtgacaat gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa
     5641 aatcaaagat gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac
     5701 gaaagactca agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc
     5761 aacaaaagaa tcttcaactg aagaaacaac tgattcatca aaa
//
LOCUS       DACDOA010000134         5668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDOA010000134 DACDOA010000000
VERSION     DACDOA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.135"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(230..596)
                     /gene="ssrA"
                     /locus_tag="IWI39_002563"
     tmRNA           complement(230..596)
                     /gene="ssrA"
                     /locus_tag="IWI39_002563"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(767..1540)
                     /locus_tag="IWI39_002564"
     CDS             complement(767..1540)
                     /locus_tag="IWI39_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9209598.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1684..2034
                     /locus_tag="IWI39_002565"
     CDS             1684..2034
                     /locus_tag="IWI39_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9209599.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2015..2731
                     /locus_tag="IWI39_002566"
     CDS             2015..2731
                     /locus_tag="IWI39_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209600.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2731..4179
                     /locus_tag="IWI39_002567"
     CDS             2731..4179
                     /locus_tag="IWI39_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9209601.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4250..4759)
                     /locus_tag="IWI39_002568"
     CDS             complement(4250..4759)
                     /locus_tag="IWI39_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209602.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4799..5473)
                     /locus_tag="IWI39_002569"
                     /pseudo
     CDS             complement(4799..5473)
                     /locus_tag="IWI39_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1935 a          982 c         1119 g         1632 t
ORIGIN      
        1 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
       61 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      121 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      181 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      241 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      301 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      361 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      421 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      481 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      541 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      601 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
      661 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
      721 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
      781 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
      841 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
      901 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
      961 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1021 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1081 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1141 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1201 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1261 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1321 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1381 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1441 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1501 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1561 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1621 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     1681 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     1741 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     1801 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     1861 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     1921 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     1981 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2041 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2101 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2161 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2221 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2281 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2341 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2401 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2461 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2521 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2581 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     2641 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     2701 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     2761 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     2821 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     2881 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     2941 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3001 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3061 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3121 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3181 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3241 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3301 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3361 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3421 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3481 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3541 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3601 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     3661 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     3721 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     3781 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     3841 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     3901 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     3961 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4021 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4081 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4141 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4201 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4261 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4321 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4381 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4441 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4501 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4561 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4621 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     4681 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     4741 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaag
     4801 tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa
     4861 atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct
     4921 gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca
     4981 gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact
     5041 ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct
     5101 gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca
     5161 attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata
     5221 gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa
     5281 gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta
     5341 aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg
     5401 aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc
     5461 ggattatttt catacttttc acctctataa aaagtataga aaatgaatat gaataaagta
     5521 tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag
     5581 gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt
     5641 cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DACDOA010000135         5301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDOA010000135 DACDOA010000000
VERSION     DACDOA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.137"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IWI39_002570"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IWI39_002570"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9209603.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="IWI39_002571"
     CDS             complement(1297..2133)
                     /locus_tag="IWI39_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209604.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="IWI39_002572"
     CDS             complement(2317..3534)
                     /locus_tag="IWI39_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9209605.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3917..4000
                     /locus_tag="IWI39_002573"
     tRNA            3917..4000
                     /locus_tag="IWI39_002573"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5237)
                     /locus_tag="IWI39_002574"
                     /pseudo
     CDS             complement(4240..5237)
                     /locus_tag="IWI39_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1647 a         1080 c          838 g         1736 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt c
//
LOCUS       DACDOA010000136         5275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDOA010000136 DACDOA010000000
VERSION     DACDOA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.138"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(92..433)
                     /locus_tag="IWI39_002575"
     CDS             complement(92..433)
                     /locus_tag="IWI39_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9209606.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(420..749)
                     /locus_tag="IWI39_002576"
     CDS             complement(420..749)
                     /locus_tag="IWI39_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209607.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(1340..1672)
                     /locus_tag="IWI39_002577"
     CDS             complement(1340..1672)
                     /locus_tag="IWI39_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9209608.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2509..3549)
                     /locus_tag="IWI39_002578"
     CDS             complement(2509..3549)
                     /locus_tag="IWI39_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9209609.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(3898..4224)
                     /locus_tag="IWI39_002579"
     CDS             complement(3898..4224)
                     /locus_tag="IWI39_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209610.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(4211..4999)
                     /locus_tag="IWI39_002580"
     CDS             complement(4211..4999)
                     /locus_tag="IWI39_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9209611.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1599 a          968 c          724 g         1984 t
ORIGIN      
        1 tccttactca atgcctaagg taatactacc tttttattaa tcgtcgtgac tccttctaag
       61 cctttatctg taaaatttat tcctccagtt tttattcttc tttatccgct acaaattcaa
      121 tgatttcaaa gctgacaata taatcactca aatatgacca agtatcttca acagataatt
      181 cgtctccatt atcattcata agcgtcattt taaaccattc attaccttca tcatcttcag
      241 gtagcaagcc gaatccaatt ggattatcag tcaatttttg atcgtataaa tcatcctcgt
      301 ctaatgctaa aacatctaat cctttacaat ttccgcctct tctaaacgtc acaaatcctt
      361 tgtaatccca acgttggaaa gaaaccttaa tcgtatgtgt gcccaatcgt atattcggat
      421 cataattttt catttgttgt aacctcctca taatcagtta gtactataaa ggtatctttg
      481 tttttatcca aaaaatctaa taaatctttt gtagaagata tctcttcatc aacttcacca
      541 taataaatac cttcgataat tgttgctata tctgaaaaac taatttttac tggacattta
      601 tcgtctaccg cattcaatac cgacaaaatt gcttcttttt ctgcaatctc tcgtttgaat
      661 ttctttggag ctaatcgcat gacactcaag tcattttcaa gcttttcaat ccatgcagtt
      721 ttactcattt tcgcaaattt ttgaatcatt cctctaccac ctttgctatc tactaaatta
      781 gttttacttc ataacgaata tctttatcgt ttttcgttca ctcaaaaata tataaaataa
      841 atcgactcgt ttatggcgtc cgaaacgaga aaccctagta gacagcggta aatgctcgat
      901 tgatccaatg atcaacgtcc tcgcaagtat ctacatgtac tgcagcacac gacacaattt
      961 cttgtgcgtc ctccccatta cgagaagacc tactccttat tccatgagtt tgcgtcaaag
     1021 cctagatccc ttagtagctg ctcatgatta atctaggcgc tattctaact tgtctaactt
     1081 ctaaattccc tcttgtttac tctgtgcata ttatttacac aaattcattt caccactatc
     1141 caaacaaggg catttactcg attgaccaat tttttacgtt accaatcgta aaaacctacc
     1201 ccgtcactcc ccgaagggaa ttgttgatag atcaagtcta ctccttattt ttctagtgat
     1261 tctaagttcc tttagaagtc tcgacaatat tttcaagcaa gcgaaaaacg ataaaataca
     1321 agttatgaga atgatcaatt cagctgatta aacgccccat cccttttgcg gtacaacgca
     1381 agactccatt ttttcttcga ccaatcagta caccgttttg gtcataaaca tgtttgattg
     1441 ttggatatag ctcttttaaa gggataggat cttttaaaat tatttgtttt tgtttcccaa
     1501 tgtaataaat catttcttct gagactttgt ctttctggat tatatatttc ttacactgca
     1561 caacggtttt actttgacca gttgatagtt tcttcgtgat cttttctgta gcggtacacc
     1621 gtttaccgta gtgacaaata tcttcgagac tcaaaattcc tgtatatgac ataaaataat
     1681 ccacccactt actttaaata tttttaacaa gtttttcttg ttatttttca aaaatagaga
     1741 attgattttc gttcatgaca aacaaaaaag agggaacgca ttcaaacatg cgttccctct
     1801 aatttcagcg ttcgtcatac ggcaaagagc cccacggaca tttttataac attatgctta
     1861 ggcatagatt catcgtaata tcacgtattt ttaaccttaa cacagccaaa aaaagtggta
     1921 tcacttttga atatctgtta tactaaatcc gttacgggta ataccgtaac actgaaaggt
     1981 aaacgcatta aggggtatag acttaatgcc acctgtggac gtcgacgaag gttaccagcc
     2041 ttccgagtct cacagggcct ttcttttttt tgtattttca attgtccaaa tttatcgtcc
     2101 aaaagtgcag aacgaaaatc aattcatcac aagcatagca gacaattaaa tttaaaacaa
     2161 ctaaaaatat acaaaaaagt gtaattgaat attagcagca ctgcctgaaa agaagtaata
     2221 tcaggctttt cagcttgggg aaataagtga attacctctc ttcatgaatt attataactc
     2281 aaaaaagcac atatgcaaac actagttccc tatttttttg agtgaaaaat aactaaaata
     2341 tctaacgatt atgaatttaa aacctacgat tgtgattgtt ttcataaact tttaatctaa
     2401 aaactccgga atactaacta tttatttgta atatttgtaa taaaattaaa atcataaaaa
     2461 agttctaatt taaaattgat catttttata gtaattgctt tttatcaact actctaacca
     2521 attttccgta ctaactgttg gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt
     2581 tgccgcaata gctttttcga caaaccaatt ttttacataa acgaaaatta gattctgcaa
     2641 attgtttact ttttcagttt tttgtttttg ataagctttc agtaaagtca tatataatcc
     2701 tttgtcagca tcaattccat aagattccag ttcttcataa acgacaatag tttgctctaa
     2761 ttcttcagct tttttctttg cattatgaat agtttggacg gtttgttgag cttcactgta
     2821 acttgaagaa aatgttttta tcaagtttaa tacattttct ggtacaaatg tatttatact
     2881 gtcctcttgc atgatgtcga cgaagttatc tagcaaaact tgattttgta actggtcagt
     2941 ttcagtgtct attatgtgtc tattagtgtc tatagaatta gaatagaata gattgtctgc
     3001 gattttcgca attacggttg agtccttgat atgactggat tttgccgtat ttgcgatttt
     3061 cgcaattacg gtagaatcat tgatttttcg cggttttgcc gtatttgcga ttttcgcaat
     3121 tacggtggaa tcagtattgt cttcctcttc catgatttta aagacgtctt gattcgtaac
     3181 ttctggcttg cctaagtaca ataaattagg catagattca atcttaccat cacgtttatt
     3241 tactcttcct ctcttctgtt tcagaagacc tgctttttct aattcttttt tgatcttaga
     3301 tactttacct tcgcggcatt gtaacaattt cattaattct tcaactgtga atatgaagaa
     3361 tatattatca tcagaatcta cccagttatt tttgactgaa taatcaaatc gatctttaag
     3421 tagcatataa gcaatttttg catcatttga aagatccatg tatttttcgt tagtaaaaaa
     3481 taccttaggc atttgataat atttttcacg ataaatttct tttgatttgt aataattaaa
     3541 atcattcata gccaatcctc tttctgtagg atagcgtttt gttcaaaact atgttacaat
     3601 gaattaaaca aatatatcta tccactatat atttttctac tgctgataag tcgccaaact
     3661 ttgttaaagc agtagtcttt ttttatcttt ttttagttta ccttaaaaaa taaattaaag
     3721 caaaccgctc tatttcaggg tttaagctca tggtaactat atcacgatat tacaataaca
     3781 ttatatcacg atatcgtaat attacaatat gaagataagc gacttatcag ctttgatact
     3841 actgattttt ataaaaaaaa tatcacgata ttatgatatc gtgatattga atgtaaatta
     3901 aattttattt tgtttctttg ctagtttata tccttcaatc aagtattctt caggtatgga
     3961 ttttaatagc gcatcttcta taacgtctgg tttacttttg tctgattcag caatagtcgt
     4021 ccaatatttg agtgctgaca ttagaattgg atttatacga atgctatctg ctgttcttac
     4081 actacttcct tgatctttta tgactttact tatatttttc ttggttaatt cgatttcttc
     4141 tacttctttt ggtttgtagt tctttttatt tttatttcct agtagtctac ttactgcagc
     4201 ttgactgcga ttatcttctg ccatcaaaat caccaacttc ttttaaatat tcttctgtaa
     4261 atgctttata gtcatcttca gctgttgagg gtttattaaa attgattaga tctgatccag
     4321 cagcttgcgc ttcgtcaacg gttattgttc tacgaatata agttttgaaa atttttgcac
     4381 caatgactct agaaatttcg gttgctgcag tcgtcatttc tttagaaatg ttataacgct
     4441 tattatactt agtaaatagg actcctcgta cttcaagctt gtcgttcgta tattctttta
     4501 ccgtttcaat agtttcactt aactttatta gtccttttaa tgagaaatac gcagcttcaa
     4561 ctggaatgat aatttcatca gaaacagtaa atgcgttgac tgtaagcatt cctaaacttg
     4621 gtggacaatc aattataata taatcatagt tgtccttgat tggttgaagt actcttttaa
     4681 gcctgtgttc acttcctact tgagtaaatt ccctctctgc agaagaaagg gctatgtcgg
     4741 ctggcaaaac atcaattcct ttatagtcca gaatagcatc tgtaatgtca gcctcccctt
     4801 ttaatacttc ataaattgta ggttggtttg ttccatctat atcgaaacct acattatctg
     4861 tagcatttcc ctgaggatca atgtccacta aaagtacatt tttaccgtaa acaaatttca
     4921 aacatgctgc taaattaacg ctagtcgtag ttttagaaac tccacctttt tgattggcta
     4981 ctgacaatat tcttgccatt aaaatatccc ccattcatct tatattataa tataattata
     5041 tcataatatt gtgatattgc aatatcgtat atgcattata ttgtaatatt gtgtgattat
     5101 tttgttttaa agatatatat cggttcggat ataaagatta ttcatatgtt attcgaaagt
     5161 aaaataatca ctttttatta ctttaaaatt ttttattaat cataagaaat atgttttgcg
     5221 aggagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtc
//
LOCUS       DACDOA010000137         5206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDOA010000137 DACDOA010000000
VERSION     DACDOA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.139"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..1367
                     /locus_tag="IWI39_002581"
     CDS             33..1367
                     /locus_tag="IWI39_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209612.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1354..2988
                     /locus_tag="IWI39_002582"
     CDS             1354..2988
                     /locus_tag="IWI39_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209613.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="IWI39_002583"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="IWI39_002583"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9209614.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4076..5065
                     /locus_tag="IWI39_002584"
     CDS             4076..5065
                     /locus_tag="IWI39_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9209615.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1824 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttt
//
LOCUS       DACDOA010000138         5087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDOA010000138 DACDOA010000000
VERSION     DACDOA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.140"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI39_002585"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI39_002585"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP9209616.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI39_002586"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI39_002586"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP9209617.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI39_002587"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI39_002587"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP9209618.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IWI39_002588"
     CDS             complement(4028..5044)
                     /locus_tag="IWI39_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9209619.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DACDOA010000139         5012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDOA010000139 DACDOA010000000
VERSION     DACDOA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.141"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..854
                     /locus_tag="IWI39_002589"
     CDS             168..854
                     /locus_tag="IWI39_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9209620.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            856..1800
                     /locus_tag="IWI39_002590"
     CDS             856..1800
                     /locus_tag="IWI39_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9209621.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1999..2643
                     /gene="pcp"
                     /locus_tag="IWI39_002591"
     CDS             1999..2643
                     /gene="pcp"
                     /locus_tag="IWI39_002591"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9209622.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2722..3999
                     /locus_tag="IWI39_002592"
     CDS             2722..3999
                     /locus_tag="IWI39_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209623.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4257..>5012
                     /locus_tag="IWI39_002593"
     CDS             4257..>5012
                     /locus_tag="IWI39_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9209624.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1638 a          896 c         1060 g         1418 t
ORIGIN      
        1 aataaattaa aaaaagcttt accttacaat aagtaagata atttttgtga taaaaaaata
       61 acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa caactatatt ttttatgtgt
      121 tttgctttat aattaaaaaa atattgtcat tttatggagg aaaatatatg gaaaattatc
      181 ttagcctttt aggcgtcttg attgttattc tcgggtttgc atttaaatta gactcgatat
      241 tgatcgtaat ggttgcttta gttgttacag ctttgactgg cggtcttggc attcaaggaa
      301 tgttagaact tttaggtaca agttttgtaa acaatcgcgg gatggcgatt tttatcatca
      361 ttatgttggc aacaggaacg cttgaaagaa atggtctgaa agaatcagcg gcttccttga
      421 tcaagcgatt caagaaagta tctgctggta tgattgtaga tatttatggg atctttcgaa
      481 tgatctttgc ggcttttaac gtgagttttg gtggagttgc tggatttgtt cgtccaatca
      541 ttttaccgat ggcattgggg accatcgaat caaaagggtt agaagtccat cctaagcatg
      601 aagaagaact aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc tttggccaag
      661 tactattcat cggaggagca ggggcgttgc tagtccaatc aacactgaaa gatctaggat
      721 atgacgtaac attgggacag cttgctttag tggaagttcc agtggctatc acggctttag
      781 ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa tattatccag
      841 gaaaggggca ataagatgga cttttttaca aatcctgata ttttattagg cgataaatta
      901 ctggagatcc tttatatcgt gatgggactc gtattgattt acactggagt aagaaattta
      961 acagataaaa ccaatcctca tcgttatggg tcagcctttt tctggacggt cttaggggta
     1021 gttattgcag gaggaagatg gcttccggca atcgtgaacg gtggcttgat ctttgcgatg
     1081 accataccag caattgctaa gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa
     1141 tatatggaaa aaatgtcaga caaactagga atgaaaattt ttattccagc tttgagtatc
     1201 ggtgtctttg cgatcttgtt tgctctattt accaatttag gtgctttagt cggcgtgggc
     1261 gtcggtgttt ttgcagccat gctaatcttg atgttctttt ctcgagataa tcgaccaagt
     1321 acatttttag atgatgcagc agatatgctg ggcacagtag ggccgttaag catgttgcca
     1381 atgcttttag cttctctagg tgcggtattt acaagcgctg gagttgggac agtgatttcc
     1441 aatgcagtag gtacgatcgt tccagaagga aatgtgaaca tcggcatcat catttacgct
     1501 cttggcatgg tgatatttac ggtaatcatg gggaatgcat ttgcggccat caccgtgatg
     1561 acggttggga tcggtgcacc attcgtattt agtcatggag cgaatccagc gttaatagga
     1621 atggttgcat tgacttgcgg tttttgtggg accctgctga caccgatggc tgctaacttc
     1681 aatatcgtgc ctgtagcgat gctggagatg aaagacaaat acggagtgat caaaaaccag
     1741 ttgtttattg cgttatttat gctagttttc caaattgctt atatgattct atttaaataa
     1801 tagaagagag gggttgtgac agaataattg tcgcaacctc ttctaacatt gtagtggaac
     1861 actatttttt tataagaata tattaagttt gatctgttat gttatttaaa ctattgtttc
     1921 ctttatttac gctgttcaag gaaaatggta tgataaaaga taaccatatg ttaaatcaat
     1981 cggacggagg aacaacagat gaaagtacta gtaacaggtt ttgatccatt tggtggggac
     2041 aaagtgaatc cagcctatga agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa
     2101 atcatcaaag tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc
     2161 atccaacagc accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca
     2221 gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga taatgaagga
     2281 aatcagccat tcgatacggc tttagaagaa aacggtccag ccgcttactt tacctcattg
     2341 ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc taccagcgta catctcttat
     2401 acagcaggaa catttgtctg caatgatatc atgtatcgtt tgcttcattt gatcgaaaca
     2461 caattcccgg caattcgagg tgggttcatc cacgtgccat tctcacccga tcaagtgatc
     2521 gatcgtccag tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc tttgacttat
     2581 gcagttgaag cagcagtaga aaataaagaa gatatttccg gaaatgccgg aacaactcac
     2641 taatttagca agaaacactg gctggttgct aacgttatgt tagtagccag ctttttgtaa
     2701 ttagaaagga aagaaacagc cgtggaagaa tggcaatccg tctttgaaga atggtttcca
     2761 aaagaaatca gcaaaagcta tcccatcaaa atttctaaac aatacacaag cagtcaacgt
     2821 tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga
     2881 tatctaatca gctctcgttt tatggaagaa cattatttag ctgcaacaga ttgggtgttt
     2941 tctgacttca agatcaatcc gttttttcga acaaaacgaa gccagcaaaa attgtattgt
     3001 gagtgtggta gagaattaaa agtgcaatac atcgttaaat cgccaaaaac cggaaagatc
     3061 ctaaaactag gcatcaacca ctttgccgat cacctgcatg tctctccgac agtggcagct
     3121 tcgatccatc agggaatgac aaaagttgat ctggccttag acgaattatt atggcttaag
     3181 cagaagaata tcgattttcc tgaaggacta tggcaaaaat actgttttgt cttgtaccaa
     3241 aatcgtcgga tgaaacagcc atatttaccg gatataaaat tagctcagcg gctcgcagaa
     3301 tttcgccaag tagaaatgcc gatttacata gcggattatc aagcattgga aaatgaaatc
     3361 aagaaaataa gtgaacacat caatgggcag tcaaaaaagc gacagataaa aaaagaacta
     3421 tttgacgact ttgcagaaga attagtaaag gatgtagaag aattcttgat caactatcgc
     3481 gcatttttac gtaaagattg gcaatccatc gtatatgaag aagtgccagt acatcctaat
     3541 gcttattttg aaacatttat ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa
     3601 gtcactgctc agatggaata ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat
     3661 ctatttattt ggaagcaata ttgtcgctat ggttttacag aagggttctt cgacagcatt
     3721 cctcggatag tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga agctatacaa
     3781 tctgctgata agaaagaccg tacagtctca aaagaaaaat ggcaactagt agtgaaagat
     3841 atccaatctg gaaatgtgca agagacaatc gacaaatgga aaggaaaaca ttatcgtttt
     3901 actgaagctc agaaacaagc gttggaatat tatcagaagt tagaagaatc actgcgtttc
     3961 aatgatgagg caagaaagta tctgaaggaa ttactataaa aaacatacta agattagagg
     4021 ggaacatcat tgacgaatga tgttctcctc tattttctat ttttagatta tagtgaagag
     4081 tgaaagaaag ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa ccgaacttct
     4141 ttactgtcat tttagaatca ggtttacgta tgttggggcc atgagcccgt gtataggaaa
     4201 ttggaatcga aatgaagcag taaagttctt tcaaaactta aaaaggagga aaacaaatgc
     4261 cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta tacaaagacg
     4321 acacctgctt atttgaagac gagattgaac acactcaaag aggatttcat gcattactcg
     4381 aagagatcca gcacttgcct gagcgtccat tgattgtttt tgaagcaacg gggatttatt
     4441 ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg ttaaacccgt
     4501 tagaggcgaa aaaacagatg gaagaaagca ccttacgcag ttggaaaaca gataaatcgg
     4561 atgcccaccg gctcgcacag actcatctta aaaatcaacg tcagccaaaa gaagtacaaa
     4621 gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt gagaagaaaa
     4681 tcactcgtct gcgcatggat ttacacaact gccttcaatt gacttttcca gagcttgaac
     4741 agttcttttc aaacaggctg accccttacg cattgacatt gatccgtctt tttccccatc
     4801 cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc aacgaaacac
     4861 ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc cactatgcac
     4921 agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg atctattacg
     4981 ccgaacttct ccaagattta ctcgaacaaa aa
//
LOCUS       DACDOA010000140         4931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDOA010000140 DACDOA010000000
VERSION     DACDOA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.142"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            84..971
                     /locus_tag="IWI39_002594"
     CDS             84..971
                     /locus_tag="IWI39_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9209625.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="IWI39_002595"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="IWI39_002595"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9209626.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="IWI39_002596"
     CDS             2273..2719
                     /locus_tag="IWI39_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9209627.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="IWI39_002597"
     CDS             2992..4344
                     /locus_tag="IWI39_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9209628.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="IWI39_002598"
     CDS             4515..4802
                     /locus_tag="IWI39_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209629.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1394 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 accccttttt t
//
LOCUS       DACDOA010000141         4886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDOA010000141 DACDOA010000000
VERSION     DACDOA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.143"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            21..494
                     /locus_tag="IWI39_002599"
     CDS             21..494
                     /locus_tag="IWI39_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9209630.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            590..1477
                     /gene="xerD"
                     /locus_tag="IWI39_002600"
     CDS             590..1477
                     /gene="xerD"
                     /locus_tag="IWI39_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9209631.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1496..1867
                     /locus_tag="IWI39_002601"
     CDS             1496..1867
                     /locus_tag="IWI39_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9209632.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1837..2634
                     /locus_tag="IWI39_002602"
     CDS             1837..2634
                     /locus_tag="IWI39_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9209633.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2618..3187
                     /gene="scpB"
                     /locus_tag="IWI39_002603"
     CDS             2618..3187
                     /gene="scpB"
                     /locus_tag="IWI39_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9209634.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            3193..3909
                     /locus_tag="IWI39_002604"
     CDS             3193..3909
                     /locus_tag="IWI39_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9209635.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3991..4116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4239..4850
                     /locus_tag="IWI39_002605"
     CDS             4239..4850
                     /locus_tag="IWI39_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9209636.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1639 a          843 c         1076 g         1328 t
ORIGIN      
        1 aaattgttgg ggttgagtaa atggtcatga atgctatttc ggcgatgaaa aaaacaaaac
       61 agcagctaca tgaagcgggg tttaagctga caccgcaaag agaagctact ctgcttgtgc
      121 ttttggaaaa tgagaaagag catttatctg cagaagaaat cttcttttta gtgaaaaaaa
      181 agagtccgga aattggactt gctactgttt atcgaaccct tgaaatcttg acagaattga
      241 aaattgtcga taaagtaagt tttaatgatg gattagcccg ttatgatttg agaaaagaag
      301 gcgccaaaca tttccatcat catttactat gcttgaactg cggcacgatc gaggaagtag
      361 aagaggatct tttacttgaa gtggagcaag tagtagaaga acgttatcat tttttagtaa
      421 aagatcatcg gctaacgttc catggaatct gtcaggaatg ctatgaaaaa gaaaaaagaa
      481 accagtccgt ctgagtgtct agacggcttt ttctttgctc agattatgga aaaagactat
      541 cagaatgaag cggtatctga tatgatagag aggcatgagg gggattatta tgaaagagca
      601 aatcaacgat tatctccact acttgaatat tgaacgggga ttgtccgcca atacacgaca
      661 aagctatgaa cgtgatttgg aacagtactt gcattttcta caggagcaga aaattgcttc
      721 atggcaggat gtcgatcgct ttttagtcat ccgttttctg gagaagttgc atgaagaaaa
      781 aaaagcgaca gcaactgtta ctcggatgat ctcaagtctc agaagattcc atcagttttt
      841 acggcaagag cgtatcactg atcatgatcc gatgcaacat atcgatacgc cgaagaaagc
      901 acagaaatta ccaagtacat tgactctgac agaagtagaa aagctgattg aagcacctga
      961 tacgacaaaa acgttaggga taagagatcg tgcaatcttg gaagtcatgt atgctactgg
     1021 gatgcgcgtc agtgaattga ttggattgaa attgggagat cttcatcttt ctcttggact
     1081 ggtacagaca gttggaaaag gagataaaga gcggatcatt cctttaggtg attatgcgat
     1141 ccaatggctg gaacgctact tagacgaagc acgtccatta cttgtaaagg acgcatctga
     1201 aatgcatgta tttgtcaata atcatgggaa aggtctttct cggcaaggaa tctggaaaaa
     1261 tctgaagcag ttagtacgag aggctgggat ttacaaagaa gtaacaccac atacgctgcg
     1321 gcatagcttt gccacacatc tgctagaaaa tggagctgat ttgcggacag tacaggaatt
     1381 gttaggtcac gcggatatct ctacgactca gatttataca catatcacga agaaaagaat
     1441 gacggatgtt tataagcagc attttccaag ggcttagtca taggaaggtg agaagatgtt
     1501 aacacattac cggaaagaaa accgtaaaat tgctatggga ctattgagtt tccataagaa
     1561 actgacaggt aaaacttctt tgttgaagga gattgatacg tacgaatctg aagacgatta
     1621 tgaacttttt ttctttacgc cagaaggttc aaccaacatt caagggatta ttggtattta
     1681 ctgggcaacg aacgatcagc tgatcatcca tgacatttct ttgaatccgt cttatcgcgg
     1741 tgagaatatg ggctttcaaa tgttagatga attacaggaa aagtacccgg atgtctccat
     1801 ccgtgcaact gaaatcacag agccttacat tggaaaatgg ctagcaaaga aaaagaagaa
     1861 agagtgaatg agttgacgga gatcaatatt aaattagatg tattcgaggg tcctctcgat
     1921 ctgctgcttc atctgattca gcaaatggaa atcgatatat atgatatacc gatcgctacg
     1981 atcacggagc aatatatgaa ttatatccat acaatgaaaa cactcgagct agctgttgca
     2041 ggagaatact tagtgatggc tgctacattg atggcaatca aaagcaagac attgcttcct
     2101 gtccaagaat tcgtcacaga cgatgaagaa ttatgggaag aagatcctcg agaagaattg
     2161 gtcactcagc ttttagaata ccgtaaatat aaatatgcag ctgaaagact gacagaaaaa
     2221 gcagaggaac gaagtcttta ttacacaaaa gaacctatga acatcgatgg gttagcggat
     2281 gctgaaaagc cgttgaagcg aggacaagtc aaaaaaatcg atttgatccg tgccatgcag
     2341 aaaatcttag aacgtaagaa attttctcaa aaagtggaaa aaacgatcac gcccgatgac
     2401 acaacgatcg aagaacgaat ggtatttatc gaagagcgta taaaaagaaa tgcgcatggc
     2461 tgtactttcg attctttttt tgagtcacct tctaaaagtg aagttgtcac tacttttatg
     2521 gctttgctag aattgatgaa aaaagggcag atcattgcgc aacaaaaaga aaactatgaa
     2581 tcaattatgc tttatccagc agtaggagat accgaacatg acgaaattaa gtgaacttga
     2641 agcccttctt tttattgttg gcgaggaagg gatcagtaat gaagaattaa gcggactgtt
     2701 aaatctcagc gaagcagaag tcacaagttt agtgaaagaa ttaaaagaat cttacactca
     2761 aaactcgcac tcagctttgc atatttttga aacggaaagc cattatgtat taacgactaa
     2821 aaaagagctg gctcccttac ttaagcgata tgcgcaaggg acatcaaaca cattatctca
     2881 agccgccgtc gaaaccttag caatcatcgc ttataaacag ccaatcacaa gagctgaaat
     2941 cgagcagatt cgaggagttc aggtttcagg agcggtacag cgtctagctg cccataaact
     3001 gattgaagaa aaaggtagag tagaaggacc aggtcggccg atattatatg gaacaactgg
     3061 ctacttctta gattattttg gtttgaaatc attagaagaa ctgcctgata cacaagaaat
     3121 ggaaatttca cttgaagaag acgtgtcacc agatctattc tttgatggaa tgaataaagg
     3181 agaataattg taatggagag attgcagaaa gtaattgccc acgctggcat cacttctcgt
     3241 cgaaaagcag aagaattcat ccttgcagga cgtgtaaaag taaacggaca agttgtaagg
     3301 gaattaggga taaaagttgg gaaacatgac actgtggaag tggatgaagt cccaatttat
     3361 caagaagaat acggttacta tttattgtac aaacctaaag gagttatttc tgctgttgca
     3421 gatgacaaag gaagaaaagt tgttacggat cttttgccga tggtaaaaga acgtatctat
     3481 cctgtaggtc gactggatta cgatacttcg gggatcttgc tactgacaaa tgatggagat
     3541 tttgctcagc gcctgacaca tccaaaacat gaagtggata aagtttacgt tgcaaaggta
     3601 aaagggatcg ctacaaaatc gatgcttgca cctttgacaa aaggaataaa gatcgaagga
     3661 cgaaaaacat ctccagctaa attccaaatt ttatcagtgg atcctaagac aaaccacagt
     3721 atcgtggaat tgacgatcca cgaaggacgt aatcatcaag tgaaaaatat gcttcaagca
     3781 gttggtcttc ctgtgcaaaa actaaaacgt gaaaaatacg gagatctgac tttacaaggc
     3841 ctacgtcctg gggattaccg tgaactgaac aaaaaagaag tcagcagttt gctgaaccac
     3901 tcaaaatgaa cacttacttt ttataagtgt tagatgttgc gttttttgaa tagatatgta
     3961 taatgaatat gtaaacaaaa attatataag gttcgtcttc aggggcaggg tgaaattccc
     4021 gaccggtggt tatagtccac gacctatttt gcaaaaaaat agctgaatcg gtgaaattcc
     4081 gataccgaca gtatagtctg gataaagaag atagagctta tttgacgacg cttgatcaaa
     4141 taactctaac tctattttcc ctggaaaata gagttttttc atttggagag aaacgtcaaa
     4201 aagttcgctg aagaggagtc ctaacaggag gatttcaaat gaagaacacc cgtgtacaga
     4261 aaatggttgc agtagcgatg tttgctgcga tcgcattagt tttacagtat tttgcttttc
     4321 cgatcatgcc ggcttttagt tttttgaaga tcgattttag tgatatctcg gtattgatca
     4381 gcatgttctt attcggtcca gcggccggag ttgcaacagc gttcttgcgt tcagtattac
     4441 atctgctgac tacaggtttt tctccggata atttggtagg agacactgct agtttcttat
     4501 caacaacgat ctttacattc ccgatttatt atttctttag tgtaaagaaa cagcataaag
     4561 gaagaaacaa atttttaggt gtcattacag gaaccatcgc tctaactatt tttatgagta
     4621 tcgcaaatta tttcgttatt acgcctcttt acttaatgtt tttagggctg aacgcaacac
     4681 agatgctggg catgtcttta gcaaactatg tactgattgg gatcgttcct ttcaacttga
     4741 tcaaaggatt catcgtgagt gctgtctttt tagtgctgca tgcgcaactt cttccatggt
     4801 taagtcgaaa acaacatcag ttcgaacaac gtcatacagc agtcaaataa atatcaaaaa
     4861 gatcaagaaa tttcttggtc tttttt
//
LOCUS       DACDOA010000142         4772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDOA010000142 DACDOA010000000
VERSION     DACDOA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.145"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(474..977)
                     /locus_tag="IWI39_002606"
     CDS             complement(474..977)
                     /locus_tag="IWI39_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9209637.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1336..2361
                     /locus_tag="IWI39_002607"
     CDS             1336..2361
                     /locus_tag="IWI39_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9209638.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2684..3562
                     /locus_tag="IWI39_002608"
     CDS             2684..3562
                     /locus_tag="IWI39_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209639.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4038..4154
                     /locus_tag="IWI39_002609"
     CDS             4038..4154
                     /locus_tag="IWI39_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9209640.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1592 a          765 c          863 g         1552 t
ORIGIN      
        1 acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc
       61 tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg
      121 gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt
      181 tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat
      241 tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt
      301 tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat
      361 aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc
      421 gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga
      481 agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat
      541 cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga
      601 taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct
      661 acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc
      721 cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga
      781 aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa
      841 gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta
      901 aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat
      961 tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg
     1021 aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa
     1081 gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa
     1141 aagttgctat cttttttctg tcaagacgac tcggaatgtt cgttttttgt tctaatttct
     1201 ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta
     1261 ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta
     1321 ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca agataatcct
     1381 tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat
     1441 catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa
     1501 aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt
     1561 gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct
     1621 ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc
     1681 ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta
     1741 ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt
     1801 acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga
     1861 tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt
     1921 tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt
     1981 gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg
     2041 attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag
     2101 ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga
     2161 aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt
     2221 cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt
     2281 ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt
     2341 gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat
     2401 ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat
     2461 tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat
     2521 tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt
     2581 cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt
     2641 atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat
     2701 gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt
     2761 aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga
     2821 ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa
     2881 gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc
     2941 agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt
     3001 ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta
     3061 tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg
     3121 tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg
     3181 aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag
     3241 agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca
     3301 agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga
     3361 gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga
     3421 tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag
     3481 tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg
     3541 cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca
     3601 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
     3661 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
     3721 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca
     3781 cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta acggacaaga
     3841 gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca
     3901 atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat
     3961 ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa
     4021 gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa
     4081 gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata
     4141 gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg
     4201 ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat
     4261 ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc
     4321 cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta
     4381 gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat cactttacct
     4441 tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata
     4501 atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg
     4561 caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag
     4621 ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa
     4681 aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact
     4741 attgctagaa taacatcaag gaataataag tt
//
LOCUS       DACDOA010000143         4749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDOA010000143 DACDOA010000000
VERSION     DACDOA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.147"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IWI39_002610"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IWI39_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9209641.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IWI39_002611"
     CDS             complement(2066..3064)
                     /locus_tag="IWI39_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9209642.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IWI39_002612"
     CDS             complement(3290..3943)
                     /locus_tag="IWI39_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9209643.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IWI39_002613"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IWI39_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9209644.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1391 a         1000 c          743 g         1615 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctat
//
LOCUS       DACDOA010000144         4749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDOA010000144 DACDOA010000000
VERSION     DACDOA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.148"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(228..1190)
                     /locus_tag="IWI39_002614"
     CDS             complement(228..1190)
                     /locus_tag="IWI39_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9209645.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGESI"
     gene            complement(1192..2631)
                     /locus_tag="IWI39_002615"
     CDS             complement(1192..2631)
                     /locus_tag="IWI39_002615"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9209646.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2816..>4749
                     /locus_tag="IWI39_002616"
     CDS             2816..>4749
                     /locus_tag="IWI39_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9209647.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLL"
BASE COUNT         1496 a          809 c          787 g         1657 t
ORIGIN      
        1 ttttgatcct ttttatttga ttaattatat ccagctggat aataattttg ggggcaacat
       61 ttctatctta ctattatctt cctaaagtag tagactttct tattgggaaa aattacaaca
      121 aaaaggagtg gcttagttga caactccttt ttgtttacaa atcaattgga tatctacaaa
      181 ttgtattttc taacggattt tatagacatt aaaaaattaa tttgacgtta aatactttct
      241 ccagaaagta ttgagattgg taaaatataa tccttatttg tcttttcgcc tttaattttt
      301 ttcagaagaa tatcaacaat aagacttgca atttcatcaa ttggttgtct tattgttgtc
      361 aattgtggga aaaatttctc aatgaaagag gtaccatcgt atccaataat ttttaaatct
      421 tctgggatat taagtttaag ttgatgagca attttcatag tgagaatggc tgttaaatca
      481 tcagaaacaa aaataccatc tggtttgtga tttataatag ttgactttat ttccatttca
      541 cgacggatag tagacagatt attaggaatt ttgaaaattt ttccatttgg attttgaaaa
      601 gaaaaaccta gtgcacgtaa ttctgtggga gaatccgtgt tatcatttcc tgtaatcata
      661 atggtatttt gacaaccatt tttttgtaaa gtcttagctg caagttttcc cccttcaaag
      721 ttatctgaag aaacaatgga aacattagga gctaaatttc tatcaaaagc aacaataggt
      781 gctctcacac gctcataatc agcaattcca agattatggc ttgatgagat tatgccatca
      841 acttggttgg cttcaagcat ttcaatgtat tctctctctt tagcaggatc attttgacta
      901 ttgcaaataa tcgttttata tccatgttta aataactcta tttcgagata ctcaatgagt
      961 tcagagtaaa agatattact aatattagga aaaataagac caataagctg aggtgacttt
     1021 ccttgaagtc cacgtgctaa attatttggt ttataaccaa gagttttcat agcttcatga
     1081 actttttgtt ttgttttttc tgataaatat cccttattat taatgacgcg tgaaacggtt
     1141 gttgggctaa ctccagctaa ttcagcgaca tcagttaatt ttgctaccat tttaatattt
     1201 taattcaaag tattttccag taggttcgcc tgatttgatt tgaataccat tttggtgatt
     1261 ttcagggaat acacgactag taaacacttt ttctccttta ttaataaaaa tctcaatgat
     1321 tgagttatct acaaaaatgt tagcagttgt ttctttagta tcaattgagc attcacgaat
     1381 cgtaccaaag tcagtggcat attgaatgcc tgctttgctg cgatctaaaa taatttttcc
     1441 ttctttggta tctacaatca agctaagtcc atttccttta ctatcagaga aaagaagtaa
     1501 ctccgatttc tgatttggag gaaaagttag ttcaagctca taagtattat ttgtttctgc
     1561 ttttgctgta aattgttcag aactagcacg taatgatgta atagcttcta ctggatattg
     1621 gtaaagttta ccatctttta ttgaaagttc ttttacaaga ctcattgctc cctgataatc
     1681 atacttatca gttggataat cgatatcagg tagtccaatc caactgacaa tcagagcacg
     1741 accatcagga gtattgaatc cttgagtcgc atacgcttca aatccataat caagattttg
     1801 aatttctgaa gcgcccacaa gttttacttt ttctatgtca agcgattgac agatcttaaa
     1861 agtattcgga tagatattcc cataatttag ctcagattta tccaaccctt gaggacaata
     1921 aatgagaaca ggttttttgt ctacaaatac tagatttgga cactcaatca tgtattctga
     1981 accagtgccg ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt tattgtcatc
     2041 cgctttgtaa agtttgataa agcctgattt ttctaaattt tgtgctccaa caatcgcata
     2101 caattgtcct tcataattaa acatttgtgg atcacgaaaa tgatcagtta catcagcagg
     2161 tttatgaata agaacattct cacctttcgt aatgttgtag tctttatcca tccatgctcc
     2221 gatttgaagt gtatcacgtt cccaattttc atttcgaacg tttcctgtat agagtaagaa
     2281 tagattcccc tctatttcat atgcagaacc ggaataagcg ccgtggctat ctcgttttgt
     2341 gtctggaaga agttctgctc ctgttacatg aaaatgaact aaatcatctg attcattgtg
     2401 tacccattct ttcaacccat gagcagctcc aaatggccaa ttttgataaa aaagcgtata
     2461 ttttccgtta aaaaaagaaa aaccattagg atcgtttaac aatcctgttt taggttcgat
     2521 atgatagcta gcacgccaag gtgatcgttt tacattatcc ttaattttat ccatctcatc
     2581 tttagtccaa tcagaataag cacgatatcg tatttctcga ggtaagttca tattttactc
     2641 cttcaattta tctttttaca actagtatgg taaacgtttt ccgttgaaaa atcaatcatt
     2701 tcatttaaaa aaataaacta tatgacaaac gtttgacata tttttaagat atgctaaaat
     2761 aaatttgcat ttcagtgaac cgttttcacg taaaaataaa aaaggagtga ttcgaatgaa
     2821 ttacagtaaa gtagcttccg acgtaataca agcggtcgga aaagataacc ttattgcagc
     2881 tgcacactgc gctacacgtc ttcgtctcgt actaaaagat gacacgaaag tgaatcaaaa
     2941 agctttagac gaaaatccag atgtgaaaag cacatttaaa attgatggac aatatcaagt
     3001 gattatcggt gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa aaactggact
     3061 ttcggaaatt tcaacagatg atttaaaaca aattgctagt aaagataaaa aattcaaccc
     3121 cattatggca ttaattaaat tattatcaga tatttttgtt ccaattatac ctgcccttgt
     3181 tgcaggtggt ttattaatgg cattacaaaa ctttttaaca gctgctggac tttttggacc
     3241 aaaatcaatt gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga tccaactgat
     3301 gtccgctgca ccattcatgt tcttaccaat tttagtaggt atttcagctg ctaaacgttt
     3361 tggagcaaac caatttcttg gtgctgcaat tggtatgatc atgacaacac cagatcttgg
     3421 tggctcaact gaatattgga atatttttgg ttatcatgtt tcacaaacaa actatgctta
     3481 tcaagttatt ccagttttag cagctgtttg gatattatct atattagaaa aattctttca
     3541 taaaaaattg ccatcatcta ttgactttac attcacgcca ttattatcag taatgattac
     3601 tggatttttg actttcacag ttattggccc tgtaatgtta ttgttgtcta atggaattac
     3661 agacattatt gtttggctat ataacactac tggatttatt ggaatgggta tttttggagg
     3721 aacatattca cttatagtca tgactggttt acatcaatca tttccagcta ttgaaactca
     3781 attactttca gcgtggacaa atggtatcgg acatggagac tttatctttg tagttgcatc
     3841 tatggcaaac gttgctcaag gtgctgcaac atttgccatt tggttcttaa ctaaaaattc
     3901 taagacaaaa agtcttgctt cctctgcggg tgtctcagca cttcttggaa ttacagagcc
     3961 tgccttgttc ggagttaact taaaataccg ctttccattc ttttgtgctt taattggttc
     4021 aggaattgct acagcagtag caggattact taaagtagtt gctgtttcac ttggttcagc
     4081 aggtttcttg ggatttcttt caattaatgc aacatctatt ccattttacg tattgtgtga
     4141 attgattagc tttgtagttg cttttgcaat tacttacttc tacggtaaaa caaaagctgc
     4201 tggtatcttt aaagcagaag aaattgctga acaaacacca gttaattctt caaaagtagc
     4261 aacaacagat ccaatatcta ctgatgttga atcaacacaa acaaaagttt catcagaaac
     4321 aattgttagt cccatttctg gtaaagtgat tagtctttct tcagtaaatg atccagtctt
     4381 ttcatcagaa gctatgggaa aagggattgc cattaaacca aatggaaaca cggtccattc
     4441 tccagtagat ggtactattc aactcacgtt tgaaactgga catgcttatg gtattaaatc
     4501 aaataatggt gctgaagttc taattcatat tggtattgat actgtatcaa tgggaggaaa
     4561 aggatttaca caaaaagtta ctactaatca aaaagttaga aaaggcgata ttcttggagc
     4621 atttgaccgt tcaatgattg agaaagaagg tcttgatgat acaacaatga ttattgtgac
     4681 caatacttcg gattattcag aagtaaatat agtagctgaa aatacagtca ctgagggtac
     4741 tgatttact
//
LOCUS       DACDOA010000145         4675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDOA010000145 DACDOA010000000
VERSION     DACDOA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.149"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            302..2146
                     /locus_tag="IWI39_002617"
     CDS             302..2146
                     /locus_tag="IWI39_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9209648.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2202..3668
                     /locus_tag="IWI39_002618"
     CDS             2202..3668
                     /locus_tag="IWI39_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9209649.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3705..4547
                     /locus_tag="IWI39_002619"
     CDS             3705..4547
                     /locus_tag="IWI39_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209650.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1523 a          756 c         1000 g         1396 t
ORIGIN      
        1 cttagaaaaa agacaaaact tttagaacac gaaaactata ttgaacgaat aaaatgaaaa
       61 aatgattaaa aaatcatttt attatttacg tttacattct aatatgttaa attgaatata
      121 acttaaacag gcatgtgact ggtaatgcag gcaggaccta aataagttga ataacaatgt
      181 atgcgtttca atacacgttt gccttgttgt caatttattt agtttctgcc tgttttttgt
      241 gttgaggatg gccacttcga ctcaaaagtt catcactcaa aaaagaaaag agggaaacac
      301 catgaattac aaacaaacag cagaagaagt attgcagtac atcggtgggg aagaaaacat
      361 atcccatttg gagcattgct caactcgttt acgttttacg ttaaaagaca acagcaaggt
      421 aaatatgagc cagttagaaa aagtggatgg cgttatgggc gttcgccaaa atgtgcaatg
      481 ccaagtaatc attggaaacg atgttgttga agtgtatgat gaagtgaaag cgttattagg
      541 tgacaatgtt tcaaatactg aagaagcacc aaaacaaaaa caaaaattag gttcagttct
      601 attagatttt attatttcta ttttccaacc tttaattcct gcgattgcag gtggaggagt
      661 attgaagtcc ttgttgctac tagcttctat cttagggctg attagtgacc aatctcaaac
      721 ctatcagatt ttaaatttgg tcggcggggc accgctttat ttcttgccta ttctggtggc
      781 aatgactacg gctaataaat tgaaagtcaa tcaattggtt gcagtatctg ctgtcggtgc
      841 attgatcctt cctgaaatga cagctatgtt ggcggaaggt gctacattct tatcttttgg
      901 attggataat atagcgtatg catctcaagt attcccagcc atcttaactg ttttatttta
      961 tgctcaactt gaaaaatggt ttacaaaagt ttcaccaaaa ccaattcgta ttttctttgt
     1021 acctatgatg agcttattga ttacagttcc tattgctttg ttgcttctag gaccattagg
     1081 ttataatgta ggaacagctt tctcttctat cattgtgtct ttgtacaatc aatttggatg
     1141 ggttgcaacc ggagtcttag cagctgtact gccattaatg gttgtaacgg gtatgcataa
     1201 agcaatgatc ccttacgctg tttcatccat gagtgaattg ggtgcagaac ttctttatct
     1261 cccggcttca ttggcacaca atatttcaga agctggcgca tgctttgctg tcagtatcaa
     1321 aacaaaagac aaaaaattga aatctacggc tgtttctgcg ggtatatctg cattattcgg
     1381 aattactgaa ccagctcttt acggggtaac gattctgcac aaacgtgttt tatatgccgt
     1441 tatgttttct agcctgatcg ggggagcatt tgcaggaatc gttgcacttc aagcctttgc
     1501 tttagtgggt cctgggattg caagtatcac gatgttcgct gacaaagctg acccaatgaa
     1561 tctggtttgg gcatttgcag tatcggggat ttcatttgtg gttgcttttg ctgcagcgtt
     1621 ggttctttat aaagataaag aagttgtcaa tgaagttgaa actaagatgg aaacggccgt
     1681 tgtttcagat gctgttcttt caagtcccgt gaatggaaaa gtaatctcac tatcagaagt
     1741 aaaagacact gttttttcta gtggtatggt aggtgatgga gtagcaatca ttcctgaaga
     1801 agggatatta gtagctcctg aagatggaga aatcacaatg gtatttgaaa cgaagcatgc
     1861 tattggttta aaaacggcta atggtgcaga acttcttttt catattggta ttgatactgt
     1921 tcagttagaa ggaaaagggt ttgaggcatt tgttcgttct ggtgaaaaag tagccaaagg
     1981 acaaaagctg attgcatttg atattgattt aatcaaagaa gcaggattcg accccacagt
     2041 tatctgtgtg gtaaccaata aggataatta cactatccgt tcatttaacc tagaaaaatt
     2101 ggtaaccaat gatagtgatc tcttagctat cagtccgata gcttaaacac aagatagtga
     2161 aaaaaaatat attgaatgaa ggaatagagg agaatagaat aatgacaaac acaacaagtt
     2221 ttccaaaagg atttttatgg ggcggcgcga tagcagcaaa tcaagtagaa ggtgcttgga
     2281 atgtagacgg taaaggtctt tcagtagcgg atatggctac ttataaacca aatttagaca
     2341 acaaagattt tgctgctcat atgcatgtca ccctgcagtc tataaaagaa gctagtgaag
     2401 acaaatctga taaatggtac ccaaaaagaa ggggcgttga tttttatcat cactataaag
     2461 aagatttagc tttatttgcc gaaatgggct ttaaaacctt gcgcctctct attgcttgga
     2521 ctcgcttgtt ccctacagga gaagagctgg aaccgaatga aaaaggggtt gcattttatg
     2581 aaaacgtctt taaagaaatg caacgtttag gaattgagcc gattgtgaca ctatctcatt
     2641 atgaaatgcc attagcttta gcagttaaat ataatggctg ggtggagcgt cgagtaattg
     2701 atgattttgt tcgcttcgct aacgtttgtt ttgatcgctt cggcaaatac gtaaaatatt
     2761 ggctgacatt taatgagata gacagcatcc accgtcatcc atttacaaca gctggaatta
     2821 tccaagagaa aagcgcagaa ggtcaagaag ctcaagatat ctatcaagca ttgcatcatc
     2881 aatttgttgc gagtgcgtta gtgacaaaag atgctcatga aaaaattcca ggaagccaag
     2941 ttggttgtat gttaacgaaa ttgatgactt atccattaac ttgcgcacca gaagacgttg
     3001 agctgacctt gaaaaagaac ttagaaaata atttttatgc agatgttcaa gttaagggag
     3061 aatatcctgc tctaatcgaa aaggatttgg aaaatcgggg aattcagatt aaaatggaag
     3121 atgaagatcg agaaatttta aagaaacata ccgtagattt tgtttctttc agctattaca
     3181 tgtccatggc agaatcagct gatccaaacg cagaacgcac accgggaaat actgttttag
     3241 gggttaagaa tcctcatttg ccttcaacag attggggctg gcaaatcgat ccaaaaggtt
     3301 tgaaaatttc gttgatcgaa ctttatgatc gttaccaagt accgttgatg attgtggaaa
     3361 atggtatggg agcagtcgat caggtagtag atggtaaaat ccatgatccg taccgcatcg
     3421 actactttaa agcacacttc caacaaatga aagaagcaat cgatgaaggt gtagatttga
     3481 tagcttatac tagctgggga ccaattgatt tagttagtgc aggaacttct caaatgtcta
     3541 aacgatatgg atttatttat gtggatgcag acgatgaagg aaatgggaca tataaccgct
     3601 tgaaaaagga ttcgttcaac tggtacaaag aagtgatcga aacaaacggt gcatccttat
     3661 tcgagtagtc tcgatctttt taagcagcga tagggggata atgagtgatt tcaataaaaa
     3721 aagtattgaa ttcaagtgtg gtacttgttg aagaaaatgg caaagaaatg attgcattag
     3781 gtaaagggat aggctttgga cagaaagctg gggatgaaat atcaggagat aaagtagata
     3841 aaatatttct gccttttgaa gaaaagaaat cttcacaact attagagtta gttgaagaaa
     3901 tacctattga attttttgaa atcactcgta ctatcgtttt tgaagcagaa aaaggattag
     3961 ataaaaaact gaatcctgga gtctatttaa ccttaacaga ccacctgcat tttgcagttg
     4021 aacgcgcaca aaaaggggta aatattgcaa accgtttgta ctgggaaata aaaatctact
     4081 atcccaaaga atttcaaatt gggaagtacg cactcaccct tttgaacgag caatatggaa
     4141 ttgatcttcc taaagaggaa gcctcaaaca ttgctttcca tttgatcaat gcacaatcgg
     4201 aagatcctga tagcggcgct ggattcaagt atgcaaaaat gatcggcggg atcgtcaatt
     4261 tggtccggta ttcgttgcag aaagatatcg atactaattc gatccattat gcccgcttca
     4321 ttacacatgt acgtttcttt gtagaaagat attattctga tgctttgttg gatgatggtg
     4381 aggaagaatt ctacaatcaa atgtggatct tatacccgtc tgccatggaa agcgcagcta
     4441 agatcaaaga ctacattgaa aaagtttatc ataaaacaat tccgaacgat gaagttgtat
     4501 acctaggtgt ccacatcaat cggttgatga aacatgctaa tgggtgagcg ctcagtaata
     4561 ctattgatgc ttttaaaatc tttagagatg gattatagta aaaacaaggt gatttccagc
     4621 tgtttgttat ctataagaga catttaaaaa ggattaacgt aagataataa aaaat
//
LOCUS       DACDOA010000146         4652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDOA010000146 DACDOA010000000
VERSION     DACDOA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.150"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..353)
                     /locus_tag="IWI39_002620"
     CDS             complement(<1..353)
                     /locus_tag="IWI39_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9209651.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEII"
     gene            complement(376..1776)
                     /locus_tag="IWI39_002621"
     CDS             complement(376..1776)
                     /locus_tag="IWI39_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9209652.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1780..2190)
                     /locus_tag="IWI39_002622"
     CDS             complement(1780..2190)
                     /locus_tag="IWI39_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9209653.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSVVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2208..2699)
                     /locus_tag="IWI39_002623"
     CDS             complement(2208..2699)
                     /locus_tag="IWI39_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9209654.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2709..3500)
                     /locus_tag="IWI39_002624"
     CDS             complement(2709..3500)
                     /locus_tag="IWI39_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9209655.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3493..4266)
                     /locus_tag="IWI39_002625"
     CDS             complement(3493..4266)
                     /locus_tag="IWI39_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209656.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4422..>4652)
                     /locus_tag="IWI39_002626"
     CDS             complement(4422..>4652)
                     /locus_tag="IWI39_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209657.1"
                     /translation="PSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVS
                     KDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1460 a          962 c          611 g         1619 t
ORIGIN      
        1 tctataatct catgatttgg tccgtagaaa ttaaagtaac ggattccatt ttcccagaac
       61 ggtaattcca taatattttt atctaataat tcatatccta aatcttgaat tgaattgaat
      121 acctcatcta tgtcatccgt atctaatgct agatgatcaa tggctcctct acactcagta
      181 gttttatctt cccatatttc tattatcgtt ccattcaact cgaaaaagcc gactttactt
      241 cccacattca tttcttcatt aatcagacta aaccctaagg attcataaaa gatttttgaa
      301 gctccaaaat cttttgttgg aattccaata tgttgtaaac catttaattt cacttacatc
      361 atcccttcaa ttgttttaaa atccacattc ataatttatt gaatagtcta gtttatctac
      421 cttgattgct ccctccgtaa cagtcatact taatgctgtt ccgactttat catacattct
      481 agtacttaaa gcaacattat caatatataa aacagctatc gtatcatcaa caattaactt
      541 tagttcatat tctttgccta tttccaaaaa tagtggtcgt tgcagcccct tatcaaacat
      601 ttgaggccaa ggatagttcg gagtttttcc gaaagtaagc tgattattaa tcaatgaaat
      661 acgaaattcg tatccttctt gagtatcact atctacaaat aattttaaag caaactcctt
      721 tgttaacgat gtaatagtca aagttgtttc aaatagaaaa taatcttcga ttttaggaac
      781 aattactctt tctttttttc caaattcttt ctctaactta aaacttgaaa atggagtgat
      841 ttctttaaaa ttctcaatta ttttttcagg cagtttaaca ccaagagttt tgtctgtctt
      901 ttgaataatt tgatgtggaa caagtgcacc gccccaatcc cagttatttt gatcgttctc
      961 cccttctttt gttgccaccc agccagttag aaaacggcca gtttcattag ttccgactgt
     1021 tcttgctgca taataggctt ttccgtcgaa agcttcgtct actggtgatt tccaatctga
     1081 aaataatcct gctccgactc gatatttagt ctttttatct tcacaatact ctgaaaaaag
     1141 taaataccac gttccatcaa tttcaaatat atcaggcatt tcaatcatgt taaattcatt
     1201 attagcccag tagtctcctt tgaaattcca atcttttaaa tcttcggatt caaaatagac
     1261 caatctcccc gtcggctttt tcttacttgc tggttttcta gctcctagta tcaaaacgta
     1321 ttttccatga tcttgatcaa aaattgcaaa aggatcgcgc caatcattct tatcatatcc
     1381 ttcttgagga acaagttttg ctgcttcgcc atcctttttc caatgaatta aatctttaga
     1441 acttgcaatc attaacactt ctgaagtttt tccatccaac ttagctttgc gattatgtcc
     1501 agtatacaaa gctaaatact cattgattcc ttggacaata cttccagcgt aaatgtattg
     1561 atcaatgtca tcgttgccac cacgatgaat tacttccccg tattcttcaa atttcacaaa
     1621 atcagtggtt gtaactagtg accacccaaa aggatcagtt aaaggtttag gatttctcgt
     1681 atctcgttga tgaaatagat aaaaagttcc atctttataa aaaggcatgc aatccccaaa
     1741 ccaaaaatct ttaggcttgt aaaaaatttt ttgcatttat cactcatcct cttcgttagt
     1801 atgtctttgc atatctgcaa gacacgcaat tgtattcata tttcttagat tttcgatgat
     1861 atcatcgctt ttttttcctt gtaatctcaa attgacagcc tcaatcaaaa ttcctaaatt
     1921 catgccacca agaagttgaa acccattttg aaatactaca cttcttttag caaattcgtt
     1981 gaaaggtgtt ccaccaatca agtcagcaaa taccataact tcttgatttt ttacttgaga
     2041 aactattttt tcaatgtttt cacggaattc ttcaatgcct gcatctccaa gtgttaccac
     2101 atgacaattt aaattatcgc caaaaaacat ttttaatgta tctacaaatc caatggcaat
     2161 atttccatga gatgtaatta taaactccat ccactcatcc tctgttctta ataaataaaa
     2221 tcctttaaaa attcttttga atctctagga gtttgtatca ctgtcacttc aactccatca
     2281 ctaattagtt ctttaaaagc ctctctctct tcttcagtaa tatttatcgt cggacggatg
     2341 cgttctgttc cattccttct tgataaattt cctacgttta cacttgttat tggtaaaccg
     2401 ttatcaatca aaaatttaac atcgattgga gaaactgcaa caataagaac tttttgtcct
     2461 tcatatttac cagaaagaat atttttaact gctacttccc ttgtaattat tgaagttcta
     2521 acattaggtg gtgcagcgag gcgaactaca gcttttttta cgtcatcatt ggctacttca
     2581 ttattaataa ccataattct gttagcactc aattgatttg tccattgtgt tgctacttgt
     2641 ccatgaatca ttcgatcatc aatacgaaca tgtataatac ctttcatttt ttcatccata
     2701 ttcaacacct acttcaaaat atttaacgct cttaacgcaa tagaaactac taagattaat
     2761 agaattacat aaactggttt aacctttcgt cctaacaaat aatacacaat tgctactaca
     2821 catgcaggta ataaaccagg cataacttga tcaaagattt catttccggt cattgttaag
     2881 tcgccttgtt taaattcaaa gccaacattc acttttacaa ctgtggcaat gagtgcaccg
     2941 accaccatta accctaaaat tgatactgca tcagtaatag actttaattt tgtttcaatt
     3001 tgagttagta ggctcgcacc cgattctttc ccagcaaggt ataaaggata agcagtaatc
     3061 cttaataaaa ttgatgccag tatccacaaa ataattccaa caaaactacc ttccaatgcc
     3121 atattagctg caattgctcc aaaaatagtc catggaataa ctacaaatac actatctcca
     3181 attcccgcta aaggacccat tagacctgtt ttcagagcac ttactgcttc caaagactta
     3241 tttttttctt ctttttcaat tccaacattc ataccaataa tgtaaggcgc tgtatacggc
     3301 gtagtgttaa aaaattgtaa gtgagctaaa acggattgtt tcattccttc aggatcatct
     3361 ttatatgtgt ccttgataaa aggaagcata gaatacgcat aacccaaccc ttccattttt
     3421 tcataattta atgaagttgc accattgaga atccaccgga aaattacttg tttgtcgatt
     3481 tttgtcagtt tcttattcat cttcgtcaaa acctcctgtt gcagctggtg ctacttgagt
     3541 attatttgat ctatttttat atacgattag tgcgattgca gcgccaagta acgaaatccc
     3601 taaaattggt atttggaaat aaactgccaa aacgaaacca actaacaaat aggcaaagta
     3661 atcttttgta ggtaaatatt taagtagcat tcctatccca acagcaggta atagtccccc
     3721 tgctaccttt aaaccaccgg taacccattc aggaatataa ttgattgcac ttcccactaa
     3781 cgttgggcct aatatcacag ttaacaaaac aggaattcca gatacaaagc ttgtattaat
     3841 tgatgccaaa tattgcatcc actctaattt ttgataatct tctttttgtg cgaatttttc
     3901 tgccttttgt tgaaaaaaaa tgtttgttga ccactttaga acatctagtt gtacaattaa
     3961 cattgctacc ggtattgcta aagttattcc tatagaagca tcctggttag ttgagattgc
     4021 taaataagta ccgatcaatg ctgctgtttg ataatccggt actgatgcac caccaaaact
     4081 agtaatacct agtgtcatta attgcaaagt cccaccgata taaagtccag tttgaatatc
     4141 tcccataata agtccggtca agaaacctgc aacgacaggt tgggaggttc cgattttggg
     4201 cccttcctta tcccagttaa tgaaaaatcc atacaacaca actaaaaatg tttgccaaaa
     4261 cgccattttg ttctctcctt tcttcttata cttttatata agcaattact gtgccaacta
     4321 acattatcca ataaagcggg aaaatacagt aaatattaat taaatgagcg tctaaatata
     4381 ctttggacac cctttcattt ttttagacgc tagtatgcta tttagaaaat agtctgctca
     4441 aaaattgtat agattaactc atactctcct ttgggtattt caatacagta aatatctttg
     4501 atttctgaaa aaaaatcttt tgatacattt aaaaaagtat caaatagttg cgctccctct
     4561 ttcaaatgtt catttgttat tccatcacca atcataattc gttcaatcat tccagaaaga
     4621 tgtagaaata gattaattct cagaaaactt gg
//
LOCUS       DACDOA010000147         4630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDOA010000147 DACDOA010000000
VERSION     DACDOA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.151"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(983..1273)
                     /locus_tag="IWI39_002627"
     CDS             complement(983..1273)
                     /locus_tag="IWI39_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209658.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1615..1911
                     /locus_tag="IWI39_002628"
     CDS             1615..1911
                     /locus_tag="IWI39_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209659.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1918..3870
                     /locus_tag="IWI39_002629"
     CDS             1918..3870
                     /locus_tag="IWI39_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9209660.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3942..4439)
                     /gene="dfrG"
                     /locus_tag="IWI39_002630"
     CDS             complement(3942..4439)
                     /gene="dfrG"
                     /locus_tag="IWI39_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9209661.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1583 a          809 c          757 g         1481 t
ORIGIN      
        1 ataatcgtag aatttaataa tttccatagc ataaagtgtt tagaaaagaa cattaagaat
       61 agttctataa taattcttga actccatgtc ttataatgtt ctatcgaagc acccaaatag
      121 ccaagttcat ctaatctact tgcataaact acgtcatccg ctctcatagt cggcatatac
      181 atattaaaga gtaaaaagat accccaaatc cctattagta acaagatcag ttctttgtta
      241 ttcgttattt ttctattatg tttgttcatt tttatactcc ttcttatatt tcctaataga
      301 gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta
      361 attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca
      421 ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg
      481 tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga acaagatata
      541 gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt
      601 gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta
      661 cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa
      721 tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg
      781 aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt
      841 catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc
      901 tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat
      961 ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg
     1021 ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc
     1081 tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt
     1141 ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac
     1201 tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat
     1261 attttttctc cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa
     1321 gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa aatcatattg
     1381 gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt atagatttaa
     1441 gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca
     1501 actgaatttg atactcgttt tttattgtta aatcaacgtt tgttgttaat ttacaccaca
     1561 aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa
     1621 attggtgtgt taaaatggtt acaatttact aagcaaaaag gggtgcattt tcctatgcaa
     1681 tttcttgagc caaaaaataa gaacgctaag tctgtggatt gggaaatatc agagcaagta
     1741 agggtgatag ttaagcaata cgctgaatat gcagagcgta ctgaaagcga agcagtggat
     1801 gaattcttat tgaacatttt agacgataag aagtttattg aatggattgc caacaagcga
     1861 agtaataagc ggattgttga aaaaatgggt atcaaagata gagtgggttg atagacaatg
     1921 accaagctta aaagattagc cacgaaggaa gatgaaattg tagatgttaa aattccgatt
     1981 tcaaacgaag aattaaaaga tagggctaaa caatatgatt tattaactcc aaaacgattt
     2041 gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa
     2101 tatcccattc aatataatta ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag
     2161 cacaaattca aagatgtaaa agggaaacca agtcgatata aaatgactgg tagttccaaa
     2221 gataaaggtc ataaaggtat gtactgcaat gacaatccta ttggcagagg tgtatcgcaa
     2281 aactgtacag taaccccttt atctaattgg tctgttgttg aagaaattaa acgccttata
     2341 gaaatcaata gcatccaaga tgttgagcca gattatcaat ttcataaaga aggctgctcg
     2401 gaagaagaat caaccccatt caatgagccg aagcaatttt acaaaagggg gaaaagtagg
     2461 ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa
     2521 agagaagaaa ccactactta tcaccaacaa aagaacacca ttcttccaat gttcgctaag
     2581 atggttgttg gaagggtttc agtcagtcgt acttgtgata ttttaggaat tggagttgga
     2641 acctactatc ataagctgga gtggctgtat aggcgttgtt tggagttttt agaacgatat
     2701 gagacacaac cccttcaaac aaagaaattt aacgagatgt ggcttaatac ggacaagatg
     2761 cactactacc taaataacgt ccgtaagaaa ggacaaggtt caaagaaata tacggggttt
     2821 gaagacctta atatgcaaac gtatatagtt gtatctgctg aggtcttatc aagatatgtg
     2881 ttccgttctg atgttgccta tgattggaat atttcaatgg atgaattaaa tgaagataca
     2941 agaaaattca aagaagacca tctcaatact tttagtcgta aaaatgatag gttagattgg
     3001 tcttattatc cacaagaacc atccgctaac gatagcgaaa ataggaatgc ttatcttcac
     3061 gaattaggga aaattactaa taggagtaga tttgtcgatg gtttaaatgt tgatgctcct
     3121 tatactacaa ctgctcacta ttggttaatt aaacaaatgg ttaatgccga cgaatggcga
     3181 atgattagtg atgatgactt ctctattaga aacgcctttt atagagtatt cacgaaagaa
     3241 ttaaggttga gcgatgcaca tcattttatt tgccaagtta acaagacgaa aagcagaaaa
     3301 caatgcttaa aagaattcgg acaagcaaaa gcagaactgc tcgattgggg agatatcaga
     3361 ggattcaaaa caaagtttct acggactttg gcatctcatt atctaactga attactaaca
     3421 agtcatcaat tccacgaaga ggctattagt aaagatggag aaagatatcg caaatatgcc
     3481 gataacccta tcaaacatcc attagccact aaggataaag gattttattc agttgattgt
     3541 agaacggatt tatctgctct ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat
     3601 gaccattcga ccaatagctt tattcaacag ataagacgtt atatatcatc tcttgaaaga
     3661 ccattaacaa cagctcgtgg tgataagaag agctatattt acgctaactt caatccgaag
     3721 tatgctcaat ttgcgattac aattctaaga acttactaca acttttgtcg cccttttaaa
     3781 tcagcggata aaaaggtttt gacacctgct caaagattag ggattacaga taaacaattt
     3841 gattggaaag atataattta tttcaaatga caaaaagagc ttggagtaat cccaagctct
     3901 tgtgctttat ctttactgtt cttcttcaat taacgcaccc gttaactcaa taagtttttt
     3961 ctttcatata catgaaaata gtagttatac ggatttttat cattctttat ccctttttgg
     4021 gcaaatacct cattccattc ctcataattc acttctggaa aaaaagtatc tccttcgaat
     4081 tcatgatgta tttttgtgat gtacattttc tcaacataag ggaaaaacaa attataaatc
     4141 tgttctcctc cgaaaataaa aatttcttct tcgtttttac ataactcaaa aacatcttct
     4201 attgaatgaa caatttcaca accattaaag gtaaacccct tatctctcgt cagaataata
     4261 tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc
     4321 ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg
     4381 tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt
     4441 tataacacag cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt
     4501 ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata
     4561 ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat
     4621 tttgtatgta
//
LOCUS       DACDOA010000148         4602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDOA010000148 DACDOA010000000
VERSION     DACDOA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.153"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            199..1148
                     /locus_tag="IWI39_002631"
                     /pseudo
     CDS             199..1148
                     /locus_tag="IWI39_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1193..1826)
                     /locus_tag="IWI39_002632"
                     /pseudo
     CDS             complement(1193..1826)
                     /locus_tag="IWI39_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(2086..2634)
                     /locus_tag="IWI39_002633"
     CDS             complement(2086..2634)
                     /locus_tag="IWI39_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209662.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2635..3492)
                     /locus_tag="IWI39_002634"
     CDS             complement(2635..3492)
                     /locus_tag="IWI39_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9209663.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3778..4065)
                     /locus_tag="IWI39_002635"
     CDS             complement(3778..4065)
                     /locus_tag="IWI39_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9209664.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(4055..4384)
                     /locus_tag="IWI39_002636"
     CDS             complement(4055..4384)
                     /locus_tag="IWI39_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9209665.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4461..>4602)
                     /locus_tag="IWI39_002637"
     CDS             complement(4461..>4602)
                     /locus_tag="IWI39_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9209666.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDEHVVEIYSARTHYE
                     SQ"
BASE COUNT         1403 a          879 c          665 g         1655 t
ORIGIN      
        1 ttgcaggatg aggtttttta tataaacttt atttgatata aagaaaaggc attcataatt
       61 gtgctatact actaaaaaat gataatacag tgaattagat tagcgcttta ttaaattaag
      121 aggacagcat atcgatattg cttccttttt tttgctattt atttaaggga tagaagttta
      181 gaaaggactg tttataagat gaaaaatgat atagtgactt atgcaaacac aaaatgtgaa
      241 gaaattggca tcagtaattt agaagagtgc ttttttgaca caaatgacct gtcgcaattt
      301 ttaagaacgg gaagaacaaa tgccagtaaa cttgccaatg acttagttgc aaagaacgtt
      361 ctatttaaaa tcaatactcg tccagtgtta tttgccatca aagagcgctt tgaagagcac
      421 tttttgattc cggtaaaaga tgtatacgaa agtatatctg aattaaaaac agacttggag
      481 cccaattcta cagattccat tctacaaaag atgattgggt atgatatgag cttatccgat
      541 gcggtggaac aaatgaaaac tgctgtcttc tatcctggaa gagggcttcc tatgatgatc
      601 actggagata ctggagttgg aaaaagtttt tttgttcata ctgcttatca gtttatgaaa
      661 aaagtgaacg ttattcatga gaatgctcct tttaaagtac ttaattgtgc gcaataccat
      721 aataatcctg aattattgtc ggggatacta tttggctatg ttaaaggtgc ttttaccggc
      781 gcagatgaag atcgaaatgg attgttgcaa gatgcagacg gtggagtact gtttttagac
      841 gaagtacaca gattgcctcc tgaagggcaa gagaaactat ttgtttatat ggatggcgga
      901 acttattcta gagtcggtga aactatccat cacgcctcca atgtacgttt agtttttgcg
      961 actacagaag gcaaagagga ttttctggaa acctttatgc gaagaatccc tatacatatc
     1021 catatcccaa atattgagga acgaggtatt ttagaaaaaa acaaattatc gaaaatactc
     1081 tctttagcga aagcaaaaac tttagcaagg ctattgatgt ttctcctaaa gtcactagtg
     1141 ttgcataaat atagtcagaa tattctgaat tattattttc ttgaaaaaag taaaaaaaat
     1201 cgtatcccaa actttcatct acatttccag aaagatcttc atgttgataa tcaattcctg
     1261 aatcaatgac cgctaccttc acatccttag aacccttggt ttcttcccaa gcggatggca
     1321 tgctaatctc tcttaaatta gcttgtttac tccaattagg atcgttatcg atttcctaaa
     1381 gaatcagttg tatttggctc ctcaacatca ttcaacggct ctagttcaac cacatagttt
     1441 ggctctgcat aattgaccat atcagaagca ttaaatgttc ttactgcctc ttccacacta
     1501 atttcttcgt tcaatttcac taataatact tgatctcgct tattataatc gctttcattt
     1561 gtcctaagag tcaaatcttt ttccaccata acttttacac ccaataaatt ttgcggttta
     1621 tttttctctg ttgaaattct ttgtgtagcc tttaaactga caattagctc attgggtaca
     1681 tactctattt gagagtcatc taattggctc ataatccttt ctttttggtt agtatctgca
     1741 gttacttgtt tagtatgagc catgaaccca aacatactca ccccaacaac caatcctaaa
     1801 tatattttct ttttaaactt tttcatctac atctcctcct aatagtttta ttttagagtt
     1861 agctctatta cttatttact aactcaacaa cagattatca tacacttatt aatgaaatca
     1921 caatatctca ttaaaacaca ccctaaaatc ataaccgttc ctatgctcat tttttggatt
     1981 ttttaatcac gtaaccagaa aaataaatcc cctctatttc acgcaaatca aagaagaact
     2041 aagtaactac cgtttaaagg tagttactta gttcttcttt gattattatc taactttatt
     2101 attcattccc caccctttgc gcagtgagtc ttcggtatct tgttttatct gactggtagt
     2161 tcttattatt tctccacgat catctaattc tggaacaatt ggatattttt ctctgaacaa
     2221 gtctagttct ttcaccacgt cattaaacaa atcttgttta tcttcttgcg taagattctg
     2281 tttaatatac tcatctaaag cttttctaag caattcatct cttgtcatat tagtctcata
     2341 cttttctact aaaactctgg aagtcgcatt taacaaagtc gacagtttta aaggatgctt
     2401 ggtggaataa accttatcaa actttctgat tggtgcgcca actttttttg ttcttcctct
     2461 actctgtctt tcaacattgg aggaagtttt tatttcttga acttggtgca cttctaattg
     2521 cttattttca gatggtgtaa ttgcttgctg ctttttcttt gcttctgctt ctttttttat
     2581 tttggcgagt cctttgttgt tcccaaaaat atcaagtcct ttgatgtcag ccaattactc
     2641 cacctccaca taccaattct cgtgttcttc caattctttc aaaatactga taaataattc
     2701 gtggcttttg cgatcccatt gttttaagag acctttcttt gtcttctcca tcgtaatacc
     2761 tgatccgtcg tatcgtttta agcgcgcatt tttgagtaca acatttttaa tcaaatgttc
     2821 cccataaatc tcttgggctt gtttatacat ttcctgatct actgcgtcac cgctttccaa
     2881 catgaaagga ataacaccag caacatataa atttgtatca aattctttca caaaaaagtc
     2941 catatactca agatattgca tcgcatttct catcgatttt acttgggtct gtaagacgac
     3001 gattgcccaa tccgcaatgt acatcgcgga tttactatat acagaaatag aaggtgggac
     3061 atctaaatag atgacgtcaa actcatcaaa taatggttct agcttttctt tcatatattt
     3121 gaattgggat aatgaatcat tatattggtc tttgatccaa tcatcataca tttcaaagga
     3181 catgtctgct ggaagtatat agaggttatt gattacttta tagacagcct ctttaggtgg
     3241 aagattgttt atcaacacgt cagtcacagt attttcgtaa acaatcggtt caccttctcc
     3301 gcctccttct tcgttagtga agttcatatg cgttaagttc atcacatcag aagtatttcc
     3361 ttgcatatcc aagtcaatta tcaaggttct ttttccttca ttaactgctg attcaaaccc
     3421 taacatttgc gcgcacgtac ttttacctac gccaccttta aaattaccga ttacatattt
     3481 cttcgctacc acgttcctac ctcttttctt ttagcaatat tcatttgata ctattataac
     3541 atatttattt ttagttacta ccaaaaaaaa gatatatatg tactacctta taattaataa
     3601 gtaaaaggta gtacataaat aaggaaaagc tgaaatatat tgatttatag ctttcctcaa
     3661 agtaatgacg ttatttaatt tatcgagaag cagagaatac tcgcgacttt agtcatgtag
     3721 acgaatcaga ttttcgataa aaaagaagcc gtccttctta agaacgacaa caaatcacta
     3781 gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt ccacataaca atactggaat
     3841 aaccttgttt tctataatct cgtaagctac tctatatgtt gtgcccgcat atttgaaccc
     3901 actagaccaa atacctgata aatccccagt tttttgttca cccttatatg gatcaatcgc
     3961 aatttcatca taaattaaat tcaaaaattt ttctttcaat ggcttctctt ttagcttttt
     4021 taaatactta atgacatgtt ttgaaggaac gatttcataa gccaatttct gttgccaact
     4081 cctctttagt cattacttta gaattagtaa gtgtatcttc agcaatatct ctaaaggaag
     4141 aatgcatttt gttttttcta ttcttaaatt ctacaagaat ctcatcttta tcgtagcctt
     4201 ctgcaattat atcagacaaa atatcttcgc taaaatcata aaattctttt tgtggttcaa
     4261 caaattcata aagaatacca ttctctacca actttgcttc aacttcgact ccattaggaa
     4321 cattaaaatc tttaggtact gtcaacatga ttgaattgcc ttgttttctt gtttttgtaa
     4381 tcatttaaat ccctcatttc taacagtatg taattactta gtaaatacat tataccacga
     4441 gataatgtat ttacaagtaa ttactgtgac tcgtaatgcg tccttgctga ataaatttca
     4501 accacgtgtt catcttcatc taccttataa acaacccggt gttgtctatt caatcttcta
     4561 aaatacctac cttcatgtgt aggttttaac ttctcaaatg at
//
LOCUS       DACDOA010000149         4513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDOA010000149 DACDOA010000000
VERSION     DACDOA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.154"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..670)
                     /locus_tag="IWI39_002638"
     CDS             complement(38..670)
                     /locus_tag="IWI39_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209667.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(677..1744)
                     /locus_tag="IWI39_002639"
     CDS             complement(677..1744)
                     /locus_tag="IWI39_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9209668.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(1741..2472)
                     /locus_tag="IWI39_002640"
     CDS             complement(1741..2472)
                     /locus_tag="IWI39_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9209669.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(2641..3120)
                     /gene="greA"
                     /locus_tag="IWI39_002641"
     CDS             complement(2641..3120)
                     /gene="greA"
                     /locus_tag="IWI39_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9209670.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(3256..4431)
                     /gene="mltG"
                     /locus_tag="IWI39_002642"
     CDS             complement(3256..4431)
                     /gene="mltG"
                     /locus_tag="IWI39_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9209671.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1273 a          948 c          690 g         1602 t
ORIGIN      
        1 ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttga
       61 atgcatagat agcggcttgt gtacggtctt ccacttctag cttagccaat atattggaaa
      121 catgtgtttt tactgttttc agtgtaataa acaattcgtc cgctatttcc tgattacttt
      181 ttccttcaga aatcagcatt aaaatttcat tttcccgatt cgttagttct tcatgtaata
      241 caggctcatt tcgccgactc attttttcca tcatttttgt tgtcacttct ggttctaata
      301 ctcgctcgcc acgttgcgtt gcccttatag catcagcaat ttcatgtgcg gtagaggtct
      361 tcaacaaata ccctgctgca cctgcttcaa tggcaggata aactttttcg tcatcaataa
      421 aactggttac gataatgatt ttagcttctg gccaatcttt taagattgct tttgttgatt
      481 cgataccatc catttcttcc atgactaagt ccattaaaat gacgtccggt cgaagtgtca
      541 tcgccttctc atagccttgt cgtccatttt cagcttctcc aattacttca atgtcttctt
      601 gaatagataa ataaaaagag acgcctaagc gcaccatttc atggtcatct actaataaaa
      661 cttttatcat ccttatctat gcctccttca tcaaagggac cttgatttct acacttgtac
      721 cttgaccttt aaaactaatg atttttactg ttccaccaat accagctact cgttctttga
      781 tattgttcaa tccataactt cccgttttcg tttcattcat attaaaccct gttccgtcgt
      841 caataatacg taacaaaaga ttattgtcta ttttgtgcaa atatacctct aattcattgg
      901 ctttagcatg tcttaatgta tttgataaca attcttgaac gattcggaac aagtgatcct
      961 caatggaact agatagtttt acatcttcta catcccattt cagcgaaatc tggattttat
     1021 tttgcaattc cttcaataac tgctcgatac cttgctttaa acttttctct tctaaattga
     1081 ctggacgtag atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg
     1141 ctaccatttt caactgcttg cgaaacattt ctggtgtctc gcttttttct gcttgctccg
     1201 tcaatgccga catcatcatc attgctgcaa acaattgttg cgaaactgaa tcatgcagct
     1261 cacgggctaa tcgatgtctt tctaattcta aaatttcttc tttggtttgt ccatctacat
     1321 attggggacg gctcgttaca atttgtaatt cactagatac ttcaatcatt ttttctttta
     1381 tcttagtgat ttccttatca atatctttga tgtataaatc gtccgatgca cttgggatcg
     1441 gaagaagcaa caatttggat tcataattgc cagaagaaag tgcacgcagc ttttcttcga
     1501 ttttcccgta ttttgtcttt tgaataagtg ataataatag aaagacaatt aatcctacac
     1561 ctaaggaaat ggctaacaaa tggaagataa gcggaacata taataaacgt gcacgcagta
     1621 attctcccca ccaatgactt tgattgctgg cataaaaata agtgaaaagt gaaaatagaa
     1681 taataaggaa agccgcaatc cccgaataaa cagctagcat cgctctggat atttttccca
     1741 tcatattcgt atcacctcga catctccaat caaggtatta gtaataatct tcaatctacg
     1801 agggttttca tcataatcac tgctgtagat tttgatttgt tcatttttca aagaaaattg
     1861 ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaatcgga tggctacgcc
     1921 caaaggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc
     1981 ttttggcaaa agcgtattgc ccaaatctat gatagtatca ccagaaatga cggcgatgtt
     2041 gatatcgtcc cattcataga cgtcgtttcc aatacgttgg ttaccgaata attgctgtct
     2101 tttccgctca ttattatgtg tttttaattg ctctgtttgg atcatcatga tctgtttctt
     2161 tcgccaaaaa gcatttttgg ttaaatctat ccctgaaatc tctacacctt tcagaccaat
     2221 aaataaaacg gcaaatacag ccatcatcca taaagcagga ctgttgacta agctaaaaaa
     2281 aatgaccaaa cttcctaata tcaattgaaa attttgaaat ttagtacgag gatgtttgcg
     2341 catagctaga tatatattga aaataccgaa tatcactaaa agcaaaagtc cagtattgtt
     2401 tacgatttgc caaacagcaa ataacagcag tagtgcttca actaccacaa aaaaacgcca
     2461 agaactattc atcgctcttt ctccctcctg acacattctc atttcatcat acctattttt
     2521 ttaactgttg gctataggac tttagatgta agatcgtttg ttgaaatcta aaatacacaa
     2581 agagaaaaag atctagagtg tatcatcagt aagatgaact ctctagatct ttcagtcaga
     2641 ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggag tagcaatagt
     2701 tacttcatca tttttacgtt tgccaatcaa tgcttgcgca ataggagaat cattggaaat
     2761 tttgccagaa aacggatctg cttcagcgct tccgacaatc gtgtactctt cttcttctcc
     2821 atctggtagt tcgatgaatg taactgtttt accaattgat acctcatctg catctacacc
     2881 gtcgttatcg atgatttgag caaaacgaat catattttca attgttgtga ttcttccttc
     2941 aacaaatgct tgttcatctt tcgctgattc atattcagag ttttcggaaa gatctccgaa
     3001 accgcgggca attttgattc gttcaatgat ttctccgcgt ttgactgttt ttagttcttc
     3061 taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat
     3121 gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt
     3181 ccaaaaaaga ttaccacgct tttcttcaaa tgtaaatgtt tttctaaaaa tgatcaatgt
     3241 ctagagcctg tttttctaag aattatttac atatttttcc actaattcca tatgttcttc
     3301 ataagtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc
     3361 attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggtcc
     3421 aggtccatag ccggtattgg tatacaaatt atatggtgaa tctacagctg tatcttcata
     3481 cgtcactaat tctttgtgtt cacctaatgc gtataaaaca gagatatctg actgaagtgg
     3541 catatttgct ttgattcggt taaagaagac ttgtgcaatg tttttacgat cattttcttt
     3601 tacgccttct ttttcaacta gagaagctag agttaaaact tcttgtacag tcaaattttt
     3661 ctgcttgata gctgaataat agttttgcat gaccgtattc gtcttgttca ccatttcaat
     3721 cactagatcc ttaagcgaag tactcgtgta ataatcatat gtggctggga ataagtaacc
     3781 ttccaaacgg tattttgtgt tctcagcttt agatgcactt tccaacagtt ctgggaattt
     3841 ttggtgcaac tcgttaaaga aagtctcatc attcactaaa tctaaaaatt ctttcttgtc
     3901 tttaccagtt accttcgcta cccgttcagc aatctgatcg atatcgtacc cttctggaat
     3961 ggcaattttc ccatctgcaa cttttgttgg ctcagaagta ccgccttctt gcaactgttt
     4021 gccaatctcg tcaagagtca tattaggcgc taactgataa tagccagctt gaaagccagt
     4081 taaattttta aattttgtat aataattgaa aacaatcccg cttttaataa tattgtcctt
     4141 ctccaaaatc tcaccaatct gcttgttaga agagccactc gggatctcga cttggactaa
     4201 ttggtcatct gatttatcta aaggcttcaa cccgctatct acataacgat acacggtaaa
     4261 tccaagaaat ccgccaataa tcaataatgt aagagcaatc actaaaatga tcttcctcac
     4321 aatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc
     4381 cttactggct tcttttttat ctttacttgg gtgttgtttg tttcctgaca aaacaactcc
     4441 tccttactat caagttcgta actttattca ttatacatga tatttgaatt ggcgtgaatg
     4501 attttattta aat
//
LOCUS       DACDOA010000150         4489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDOA010000150 DACDOA010000000
VERSION     DACDOA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.155"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            575..1009
                     /locus_tag="IWI39_002643"
     CDS             575..1009
                     /locus_tag="IWI39_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209672.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2299..3222
                     /locus_tag="IWI39_002644"
     CDS             2299..3222
                     /locus_tag="IWI39_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9209673.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3238..3777
                     /locus_tag="IWI39_002645"
     CDS             3238..3777
                     /locus_tag="IWI39_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9209674.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1679 a          674 c          759 g         1377 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaaataa tgagagttta ttcttgaaaa agcaacgcgc
      241 ttataaagcg ctaccaaaaa tttaccatat tttatgaaaa ataacaaccc agtaaaaaat
      301 gactggtaaa aattcacaaa gaaacggaaa gaagcaactg cagaaaatca agccttaaaa
      361 agagcttttt acctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
      421 ggggctactt ggcatagtcg acattatgtc tactaacctt taaaataaca cgttttataa
      481 aataaaccct cttgacaata tttaattttc tgattattct aaatatatac agaaagcgct
      541 ttcctaggat tttatatcta aggaggaagt aacaatgaaa aataaaatta ttaaaattat
      601 tacttgtcta tttttgttcg gtatgattgt tggagcaagt actccgttaa atctgtttcc
      661 ttttagtgct agaggtataa aagtggaagc tgcaagttca cgatataatc ataatcatcg
      721 tggatttact tgctataaaa tgaactatta tgttactaat aaaatgtgta gagatttgaa
      781 aaaaaattat aataagctaa agactcctgc tcaaattgca tcttttattc ctattggtgg
      841 agttggaaca tgggttatta caaatgcttt tagtagtgct gttgatgcaa tgaatgtctt
      901 tgtaagagct gcaaatcaag gaaaaggtgt acaattaaca tataatgctc actttagcaa
      961 tacaacatca tatcaataca atgattacgc tcgttatgta ataaaataat cagaaagtga
     1021 aactatctca tgaataaaaa gattttagct gtaattattt caataatagt tttaatcact
     1081 atgttcttca tatttaggct aattttcaat ataagcttac aaaaaagtat tctttatcta
     1141 attcctatag catttatctt agcaattttt agaagtattt acggaaataa aaactctaaa
     1201 tagctaaaaa taaatcatac taaattttac aaaaagtggc tagcattttc tagccacttt
     1261 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1321 agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc ttgccaaacc
     1381 cttttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1441 ccgtaggacg tactgaaagg gtgggactta tgagcaaatt aatgcaaaat ttggctagca
     1501 aaaaagctaa aaaatatatg ctaataatcg agcgaaataa acactttttc aagtgagcga
     1561 atccgccatg acaaagctga agcagtttat ttcagctttg tcatggcgtg tgccgacttt
     1621 tttaaaagtc taggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac
     1681 gtttgaaacg cctaagtata taaatatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttatact aggactgtct taaaatgtct taaacgaacg
     1861 ctcagagctt ctcaaagggt tccttatgaa cggcttttag caaaggagcc ggaggcgact
     1921 gcgcagtata atgtgagagc agagcgatca caccttttta gcgaaccgta gggagcaagg
     1981 tttggaattg gcagttttga taaattcgcg aacctgttta gagaaaaaag gagtgatttt
     2041 ttaggcggga aaattagcct cccctctttt tatatctctt tttatatctc tttttatatc
     2101 tcttttatgt cggctgtgtc cttactctcc caagcaattc agagtttttt caagtaggtt
     2161 ttctacgctt ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag
     2221 taggttttcc gacattattg agtattttta taaagtatgt tacttttatt tcatacttta
     2281 taaaagtagg agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat
     2341 attcctctga gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt
     2401 gtacgtgaaa aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc
     2461 ggttatacag aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta
     2521 ttaagtatta atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca
     2581 aaatatagca ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa
     2641 ggacttttta atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg
     2701 gatagcacat attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg
     2761 actgaatttt ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat
     2821 agtccgtcaa atattgacaa atttatctta aggccaatta aagaagaact cactccctta
     2881 tttaaaggat taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt
     2941 tatcgtttta cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa
     3001 gaagatttac gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa
     3061 aaatggcgcg ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt
     3121 aaacaacaac aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc
     3181 aaacaagatt tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg
     3241 gcagataaaa caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac
     3301 aaaaaagttt ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg
     3361 attaacgaaa ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta
     3421 aatcgaaaac tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt
     3481 tccgaattaa aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta
     3541 caagaaaaag ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc
     3601 actctacaag cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca
     3661 gaagaaactc caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa
     3721 ccacttattg aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat
     3781 tttctagaag ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac
     3841 tcaaattttt atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt
     3901 gtaaactatc aatgtttata gttgtaaact atcaaactat atggtgactt attgcttaaa
     3961 ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta atcgattttg
     4021 ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa atcgtttgaa
     4081 atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga atcattatga
     4141 gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg gtattaaggg
     4201 cattctccgc ctcccaggtt cttgaccatt ttctctcaac gtggtaaaaa tcccacttat
     4261 aagagaaaat ggtcaagaac ctaaacggtc ggcgaagaaa acataccaag caccacaagc
     4321 aaaaaaagtt agagtaactt ccaaaaaaga aaaatttttt agaacaactt ttgaaaattt
     4381 aattttgatt tatatccgtt caatttaaac atttgataaa ctgaaggcta gtatttaaaa
     4441 tgtgtataca agtggtagcg ttaaaaaaag tggaggtata acctatgtt
//
LOCUS       DACDOA010000151         4303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDOA010000151 DACDOA010000000
VERSION     DACDOA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.157"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            568..1536
                     /locus_tag="IWI39_002646"
     CDS             568..1536
                     /locus_tag="IWI39_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209675.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1547..2362
                     /locus_tag="IWI39_002647"
     CDS             1547..2362
                     /locus_tag="IWI39_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9209676.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2430..3140
                     /locus_tag="IWI39_002648"
     CDS             2430..3140
                     /locus_tag="IWI39_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9209677.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3176..>4303
                     /locus_tag="IWI39_002649"
     CDS             3176..>4303
                     /locus_tag="IWI39_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9209678.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1448 a          645 c          901 g         1309 t
ORIGIN      
        1 tctgaatata actactgtat caaaaacaat cgattatgag acaaaacaaa atgacctctt
       61 ttatttaaga aaggaggtca ttttttgtat cataaattgt attaaaatca atgtatcact
      121 ttttaatcaa ttttaagacc ataaattgcg tactaaaaga aattctgcaa ttcaaaataa
      181 agcattttaa gccattttca aatttaagtg cacccatcta cccgtcaaat cactttattt
      241 tccactccag tagtttccta attcaactgt gtgcaaaacg atttcttcta aacaaatcaa
      301 gacaaactta taaaccttca tcccagcacc ataattattt aaatcatagg catttttcga
      361 aattagtata tgtactcaag tttcactgca gattatttat attttttaga gaaataaatt
      421 tttcctattg acaaacacaa agaaagcgga tacaatacaa ttatgtcaca tgacgttata
      481 tgacgttatg taatatcttt acatttaatc agttactttt attttggata aaattaacat
      541 ttttaaaatt ttaaggagga aatgttgatg gatgtagcaa aaacggttaa cgcaattgta
      601 gaaaaaaatg atggagagat ttctggaatt tatttggtgg cttgtggcgg ttcgctggta
      661 gatatgtatg tctcagatta ttttcttaaa gccgaagcat cgaagtgtat tacaggctta
      721 tatacagcga acgaatttaa ccatgtaacc ccaaagcaat tagatgataa atctgttgtc
      781 attctatgtt ctcatggagg aaatacagca gaaacggtaa aagcaggaga agtcgctcaa
      841 gctagaggag ctgtgactgt tggtctgact cataataaaa ctgcggattt attaaaggtt
      901 tctgatcata gtttccttta cgaatgggga gatgatgcaa aaatagtcaa taatcctatg
      961 gccatcattc ttgatttaac tgtatctttg gtcaatgtta ccgaaacatt tgaacattac
     1021 ggtaaattca gagaaggcat gaaggttatc gataccgtaa ttgaaaatgg cattaagcag
     1081 gtgcaggatc gtattaaagt gtttgctgat aagtacaaag atgaaacaat gttttatatc
     1141 ttaggtagtg gaccatcata tggacatgct tatggttttt caatttgctc tttgatggaa
     1201 atgcaatggc ttgattcaac ttctgttcat tctggagaat tcttccatgg accttttgaa
     1261 gtaacagaca aagatacgaa ttttattctc ttgatgagca ttggacgcac tcgtgcttta
     1321 gatgaacggg caaaacgatt ccttgataaa tatgcagaaa gagtagaaat cgttgatgca
     1381 aaggaactag gattggatat gatatcagat gaagtttctg aattcttcaa tcccatttta
     1441 ttctacagta ttctttcaga atatcgttca gctttagcag atgcacgcaa tcatgattta
     1501 gatgttcgtc gctatatggg gaaagtggag tactaggagg attaaaatga aggtattagg
     1561 cttaggtgat aacgtcgtcg ataaatatga aaatctggga attatgtatc ctggaggaaa
     1621 ttcattgaat tttgcagttt ttgcaaaaaa actgggacat gaaagtagct ttatgggagt
     1681 ttttggctca gatgaggagg cagctcatgt tttatcagca ataaaaaaaa taggactgga
     1741 caacagtcac tctcgctttg aaaaaggcga gaatggctgt gccagagtga agattgatga
     1801 gggcgaccgg attttccttg gcagcaattc gggaggtgtt actcgtgaat acccgattca
     1861 gcttactgaa gaagacagag aatatttaaa ccagtttgaa ttgattcata tgggattata
     1921 tagccatgtc aatcatctat tagaagagtt acaaaatgtt tctggaaaga tttcttatga
     1981 tttttctgac gactttacgg aagaagaggt tcagcgggca attgataaag ttgattttgg
     2041 cttcttttct atagaagact tttccgatga agaagtaaag cagtttttga tgaagcattt
     2101 ttgtgagcga aatgatgtat tgattgcaac ccgaggagaa aaaacggcta ttggttatga
     2161 tggagcaaag ttttatgaag tgaaccccgt gctagaagaa ccagttgata caatggcagc
     2221 gggagattct ttcttaacag ctttcctact ttattttctc gaaggggacg atatagtcac
     2281 cgcgatgaaa aaggggaatg aatttgctgg gaagtcttgt ctggttgacg gttcgttcgg
     2341 ctttggtatt cattacgatt gagcttgttt taaattcctg catttgataa cctaaagaaa
     2401 gaaatcccgg taaagtggag gttaaaaaaa tgctggatac aaatgcacaa ttaccattgt
     2461 acttacaatt aaaagaaata ttaaaaaata aaatcaatac aggggagtat cccgagaata
     2521 cgaaaattcc cgctgaatca gagctgatag aaagttatga ggtcagccga atcacagtga
     2581 gaaaagcagt agaagaactg gtagaagaag ggtacctact taaaaaacaa gggaaaggaa
     2641 cttttgtcaa ccggcataaa gtttttcgta aaattgaata tgttatgggt ttcagtgaga
     2701 gttgtaaatc taatggtttt acaccgacta gcattgtttt ggaacgcaag atcattccag
     2761 caactgaagg gatggcaaaa aaactcgata tcgctcaagg tgatgatgtt ttatatatcc
     2821 agagaaagcg aatggcggat aacatcccca ttatgttaga aaacaattat ttttcttatg
     2881 aaagatatag tttcttaatg gaggaagagc tgactgattc tttgtatgaa gtacttaaag
     2941 tccaaggtac cttagccatc aatcctgggg aaactgcact tgaaattgtc acagctgatg
     3001 accaaattgc caaaataatg gaagtagctg ttgggacgcc ctttttcttt atggatactt
     3061 taatcaacga tcaaaacaac ataccaattc atgtaggtaa tcaatattac ctgggagaag
     3121 tatatcgttt ttacttatga tttcctgttt tgatttcaag gagtagattt tatttatgaa
     3181 gcaagtattg cggtcaaaaa tgatttttga tggagagcag ccacctttca aaggttatat
     3241 tgtaatcaat gaagggatca ttgaaaaggt ggaaaacaac tggaattaca gtgatctatt
     3301 aaatgaaaac acggaattaa ttgcttatga tgatcaattc gttatgccag caattcatga
     3361 taatcatgta tttttcagtg gttatttggc tatgaatgct ggcatagatt taagcgatac
     3421 atcttcagct aacgaggctg tagagcgtat taaagctgta atgaaccata aaaaagcaga
     3481 tgaaccaatt tatgctcatg gctgggatgc tgccctgtgg aaaactgacc catcagaaga
     3541 gctattgaat gaaataaacc actttggtcc aatcacagcg atcgacaaaa atcgcagcca
     3601 ttgttggatg aatgctattg ctaagcagac ttatggtttt acagaaaaag aaaccagtgc
     3661 tgaaagtcga gtattattaa ttaaggaaat gctagcaaac aaaaagcttt tagcagaggt
     3721 ttacgctgaa tttgaagcat tactgctttc aaaaggtgtt atatcaatga aagaaatcgt
     3781 ttttgatgac gcagaatttc tttctaatat acctcagagg aaaatactta gtaacctata
     3841 tgtgcaggca gtccaagaac ctctacagca tgaacttcta tatgagtata aggaaaaagt
     3901 tttccctgaa aaggtccgct ttggtggagt gaagatcatg gtagatggtg ttgttgcgga
     3961 tagaagtgga gatatttacg gtgtttatca gtcaggagta gcttcacccg agattgacta
     4021 tgaggcaatt aaagcagaag ttgctgcatt caatgaagta gagattcctt gctgtttgac
     4081 aacggaagga gataaagcgg gttataaagc tgcgtcaatt cttgtcgaat ttggaaagcg
     4141 gttacctgaa ggcgttttta attcgattag tgatttggaa atggttactc aaaagacagc
     4201 agaaaaaatg aacgaaggac aagtagtagc agaggtatat ccacaaattc tcggcttgaa
     4261 tcaaacgtta gaagaggctt acatgtcaac tgttataaaa aat
//
LOCUS       DACDOA010000152         4204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDOA010000152 DACDOA010000000
VERSION     DACDOA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.158"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(68..1840)
                     /locus_tag="IWI39_002650"
     CDS             complement(68..1840)
                     /locus_tag="IWI39_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209679.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1794..3548)
                     /locus_tag="IWI39_002651"
     CDS             complement(1794..3548)
                     /locus_tag="IWI39_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9209680.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3658..>4204)
                     /locus_tag="IWI39_002652"
     CDS             complement(3658..>4204)
                     /locus_tag="IWI39_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9209681.1"
                     /translation="IGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVY
                     ISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQ
                     VASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLV
                     VASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1419 a          799 c          588 g         1398 t
ORIGIN      
        1 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
       61 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
      121 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcactatga gtaccttgat
      181 caactatttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
      241 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
      301 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
      361 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
      421 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
      481 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
      541 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
      601 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
      661 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
      721 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
      781 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
      841 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
      901 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
      961 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
     1021 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
     1081 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
     1141 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
     1201 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
     1261 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
     1321 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
     1381 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
     1441 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
     1501 atggtaaatt tgacaaatcg tctgcaattt gcattcttaa atgcgcactt tctttataag
     1561 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
     1621 cgaacgatac tgtaaaataa agccaaggcg gctttacatt ttctaaaact aattcgtcta
     1681 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aagctagaaa
     1741 tagatgcttt aaccaaatct tctgcgccct gattagttaa agcgaatttg tttttataaa
     1801 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
     1861 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
     1921 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
     1981 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
     2041 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
     2101 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
     2161 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
     2221 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
     2281 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
     2341 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
     2401 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
     2461 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
     2521 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
     2581 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
     2641 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
     2701 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
     2761 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
     2821 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
     2881 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
     2941 cctcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
     3001 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
     3061 attagaacca atccaatttt aaaattaatg aaaaagccta aggcaactga taaaacaggt
     3121 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
     3181 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
     3241 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
     3301 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
     3361 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactta
     3421 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
     3481 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
     3541 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
     3601 gctttccgta atagcgtaaa ttagagatgt acaaacagat agaaggtaaa ctacgttcta
     3661 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
     3721 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
     3781 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
     3841 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
     3901 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
     3961 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
     4021 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
     4081 tatgattcca gataataatc caaaaatccc tggggatatg ctgtgaccaa acgccccacc
     4141 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
     4201 aata
//
LOCUS       DACDOA010000153         4172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDOA010000153 DACDOA010000000
VERSION     DACDOA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.159"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..1375
                     /locus_tag="IWI39_002653"
     CDS             74..1375
                     /locus_tag="IWI39_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9209682.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1402..1662
                     /locus_tag="IWI39_002654"
     CDS             1402..1662
                     /locus_tag="IWI39_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9209683.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2661..3896)
                     /locus_tag="IWI39_002655"
     CDS             complement(2661..3896)
                     /locus_tag="IWI39_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9209684.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1203 a          669 c          866 g         1434 t
ORIGIN      
        1 taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa
       61 aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg tcccaccaat
      121 ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg tttcaacatt
      181 gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt ttcctggatg
      241 gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga tgacgatggg
      301 attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat catatggatt
      361 agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac aagttcctgt
      421 aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga gttctttggg
      481 agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag tatatcgttt
      541 ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg
      601 tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc tgattcgaag
      661 cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt taaccaatat
      721 tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt tgttgtggtc
      781 atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat
      841 gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat taccctatat
      901 cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag caacgatcgt
      961 ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt
     1021 ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag ctcctatgat
     1081 gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga ctattatcag
     1141 ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa catggacatt
     1201 accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg ttatcttgat
     1261 catgatcaac acattgatag catttattat ctattatcca tttgtaaaag cttatgataa
     1321 aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg
     1381 gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt tatcttttta
     1441 ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca aatcaatcga
     1501 tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat tgtaccgcat
     1561 atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc tatcaatttt
     1621 atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg
     1681 taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc ttcttaaatt
     1741 tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
     1801 tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag ccctttttta
     1861 cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa aatggaaatt
     1921 ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag tcaaatatag
     1981 atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt
     2041 atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg ctgggaatag
     2101 caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata gaacattctg
     2161 acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat gcatcggatt
     2221 gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag tggtaaatcc
     2281 tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca aagcgatgct
     2341 tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat ggtctttctt
     2401 ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg actaagaata
     2461 aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt taaggatgat
     2521 tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca ctatttactt
     2581 tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt ctctattaaa
     2641 aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc gatttcttga
     2701 attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga
     2761 atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt cactggtttt
     2821 gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct ttgctcaaat
     2881 tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac ggtcccttcc
     2941 ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga cagatattct
     3001 gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc aataggtgca
     3061 ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga
     3121 ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg ctttcgctgt
     3181 ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat tggaccaacg
     3241 attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga
     3301 tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat ttctttttgt
     3361 gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt ttttgtccag
     3421 tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat cagcgcatgc
     3481 tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa aggaatcttt
     3541 ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag tcgaggctca
     3601 aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc tttggtaaaa
     3661 gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg ttttttcgta
     3721 aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg agcaaaagtt
     3781 ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc aacctcaatc
     3841 attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt cttcatgaaa
     3901 tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa aaaacacagc
     3961 gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt tcacaacttc
     4021 taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac gcaacaattt
     4081 tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt aaaaataaac
     4141 atgagaacat aaaaaaataa aataattata tt
//
LOCUS       DACDOA010000154         4149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDOA010000154 DACDOA010000000
VERSION     DACDOA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.160"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..503
                     /locus_tag="IWI39_002656"
     CDS             75..503
                     /locus_tag="IWI39_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9209685.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IWI39_002657"
     CDS             490..732
                     /locus_tag="IWI39_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209686.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IWI39_002658"
     CDS             complement(1032..1247)
                     /locus_tag="IWI39_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209687.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IWI39_002659"
     CDS             1354..1668
                     /locus_tag="IWI39_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209688.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IWI39_002660"
     CDS             1772..2950
                     /locus_tag="IWI39_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209689.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="IWI39_002661"
     CDS             3353..3646
                     /locus_tag="IWI39_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9209690.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1415 a          619 c          812 g         1303 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgc
//
LOCUS       DACDOA010000155         4063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDOA010000155 DACDOA010000000
VERSION     DACDOA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.161"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(845..1102)
                     /locus_tag="IWI39_002662"
     CDS             complement(845..1102)
                     /locus_tag="IWI39_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9209691.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1095..1364)
                     /locus_tag="IWI39_002663"
     CDS             complement(1095..1364)
                     /locus_tag="IWI39_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9209692.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1495..1674)
                     /locus_tag="IWI39_002664"
     CDS             complement(1495..1674)
                     /locus_tag="IWI39_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209693.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2055..2762)
                     /locus_tag="IWI39_002665"
     CDS             complement(2055..2762)
                     /locus_tag="IWI39_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209694.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(2755..3192)
                     /locus_tag="IWI39_002666"
     CDS             complement(2755..3192)
                     /locus_tag="IWI39_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209695.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(3207..3458)
                     /locus_tag="IWI39_002667"
     CDS             complement(3207..3458)
                     /locus_tag="IWI39_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209696.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(3458..3664)
                     /locus_tag="IWI39_002668"
     CDS             complement(3458..3664)
                     /locus_tag="IWI39_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9209697.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
BASE COUNT         1164 a          795 c          594 g         1510 t
ORIGIN      
        1 atgcatgtaa ttaactaatt gttgagcttc agaagttata tctctaatta tatagttaac
       61 ggaattagga agtgtcttta actctaatga aatttctatc caattactat cttctaatct
      121 gtatatgctc gaagtataat ttgattcgtc agcaagcatc gctacatccc aaacactgat
      181 cataaaatct tttaaacgtt caatcatttc agcaatattg ctaaaatcag ctaatttttc
      241 tttagaataa aaaaaacatt tatatcagaa tcaataaatt ctattttttt tattactata
      301 tcgtcatcat tatcttgacc atttatgttt attgttccct gagtggggag tacttctttc
      361 aaattcaagt taaatttata ttccattact tctcctccaa tatataaaaa ttacttaact
      421 gtatccaaat ttaatatgta aataaatcat taatttttag ctaaattcta tgagaagttt
      481 atttttgttg tttttgctaa ttaactaaaa ctagtacaaa aaatttaaaa tagattatta
      541 aacctattgc ttattctatt cagtcatata tataagcgtc atcctttagt aactaacatc
      601 ccacactaca atagtattat aacatatcta ttcaacttca aattgttgaa atttaagcca
      661 tttatattta cttttcctca actttagaaa tgattttact aaccttatat ccgtaatacg
      721 cgtaacaaac acattcatta ataacaatca acactaagct atattagctt tttaaatgac
      781 tagggtaaat aaaataagga tcaaaaactt ttaagtttct gaccctttcc ttacttccga
      841 ttggttaata gtgatctttt gcagaataaa taaatatcgt ttcattttca actctatata
      901 tcagtctatg ttcatctgta attcttctgg accattttcc agataaatca tgctttaatg
      961 gctcaggttt tcctaatcca gcaaaggggg aacgatcgat atcttttatt aacttgttaa
     1021 tcttttttat attgcttttg tttccttgct catgccaata aagataatca tcccaagcat
     1081 catcagacca agccttaatc atcagattca acctcgatta agtcatgtgt tttaaatgca
     1141 cctttggaga attgttcatc tcctcgacga attttttcca tgacgtaatt attagaaagt
     1201 gttctcaacg tttcttgcat agaatcataa tctctttttg ataatacaac aactgtatct
     1261 tctacatctt tacttgttac aataagtgtt tcagcatcct cattaacttg tttcatataa
     1321 ctacgtaaat tttggcggaa atttgaataa gctactgctt ccattccttt caccccttat
     1381 ttatttctat aaaacaattg tacattatat tgtacaatta agcaaatgat taatattcta
     1441 acttcttata attaaaagtg ctatcacttt tagtttttag gataaaaagt gacgtcactt
     1501 tttgagtgct ggtacctcaa atcctgcttt ttcttgggca atcaataaat ctaacgcaat
     1561 acacataatc ctggattcac ttttgccgta cttctgcttt agttgctgca attctttttg
     1621 tctcttttca tccaacgtca cgggtaaccg tttatttcct attgcaattg gcattagtac
     1681 tctctccttt catcgaaata accctattaa aaatatggct gcaatcaact tgaatattag
     1741 actcacaagg gttaacacaa agcgccaaaa gatcaaacaa aataccagta ttcctaagaa
     1801 tactacgaag tttaaatgta gagctgcacc atgacgcata agtatctcgc caacaaacag
     1861 tacgaacaat agaggaatga ttttattcat atggatctcc ttctttctct taggttatcg
     1921 ggctttgccc gcttatttcg caccacctaa gcggcggggg cggttggatc gattggtgcg
     1981 aaataagaac cattccctta atttcaaggt tttttgttct tatttcacac atcctcactt
     2041 ttcggtataa atggttattg accttttttc ctttcaaatc gttgatatgg cattcgttta
     2101 cggtcaagtt cattttggat ttcttctgta tcgtcgtaag tgtaggtgtc taaaaaatca
     2161 gttaaagtcg tgtaccagtc gtcgcctggg ttatctactt cttctacata ttccctcaaa
     2221 aatgcttcgg gtacagtgag ttcataatcc attcccatta acacgataga ttccgtccca
     2281 cacgtcaaaa atctagtaaa tacaaggtaa taatcgtcta ccgtcgcatc tttgctgttt
     2341 agctcttcca tttcttcttc cattaattcc atgctctcaa aaattgaata atattctgat
     2401 tctgagtaat agtgtttcct gcaaatcgtg tagctgacaa agttttcgac cagctcactc
     2461 ctttctaggt aaacattcat gatatatttt tctgtttcta gtcgtagaag ctccgctcct
     2521 ttttttaatg tgtcaggacg acgtttatac acgtctaaac gattatggct gactgtaacc
     2581 ttcgtttgac tcgtttcact ttccatatac aactgttctt ccactctttg aataaagtac
     2641 ttcatttctt ccatacaact aaagtctttt tgaatccgtc tgattacttc attcaagtgt
     2701 tctttgtttg ttaaaaattg cgaaacatct tctgttgtta gctgataaac caccttattc
     2761 attatctttc tcctccgatt caatcgtata aaatgcacca tttttttcga tattttcaaa
     2821 agtatctagc cagtcgctag ccattgcttt agctttctta atctgtgcga attttgcata
     2881 agtcagttct aaggcttgat attcggaagg tttgatagtg cttggtttga cacctacttc
     2941 caagcatagt aaggcttgga aaaaccattt tccattttgt tttttcagtt cttccagtgt
     3001 tcgatagtac gtcgcaatga gattcatttc gacacctgat tcaatcattt cttccactgc
     3061 gactgacaat ttttccttgt aatgtttttt gggtacacgt gtttcatggt ataggaacgt
     3121 catatcctcc attttttgtg ccattttatc caaaggtaat ttgacaaatt cgtcatagta
     3181 attttgagcc atattttttc tcctttttag ttctctaatt tggttaacca atattcgatt
     3241 tcataagttt ccatgttttc ttgataatgc agttcatatc ctgcaatctt cactaggtct
     3301 tcaaatagca tagtcggtgc atcctctact aaaggggttt ctatcgtcca gtaccgcgga
     3361 ataagtaaag cttctgctac tttttcagaa atgacttctt tgtccaccaa aatttgaacg
     3421 acttctttta cagtcaataa tttgtgttgt gttttcatca ccgtttcttt actttctcat
     3481 caatcaaaac ataatcgcca taagacaagg tatatttttt cccgcatgtt ggacattgat
     3541 acaaacgata gcttttgata catttcgctc tttctccaca atcgcattcg acagtatgtg
     3601 gtgcatgata gtttgggaat aattcgctga agggaacacc ccaataatca aatttctttc
     3661 gcatgattga ttctgcctcc tcttaggaaa ggttttattt cctttcgtta gttctttctt
     3721 tattgatttg ttggccagaa tgaatcaacg aaaagcgaca aggacaaggg cgcgcagcgt
     3781 gccatttacc cttgtccttg tcgctttttg gtgataaaag gcaacttgtt gccggtctgg
     3841 caaatcaata aagagatagc gaacaggaac gaaccttttg gtgagaggaa gttttttcta
     3901 ttttgtcttg ctttgcaaga caccatatct tagtgaacgt gcaacgttca cttcctttct
     3961 tctcgaacgg cggtggcggt tggagtgaga gaaaggtttt tcaattgaga aggcacttgt
     4021 gtcttcctaa aaaggtcttt tcctgtttct atgtaaaaca aaa
//
LOCUS       DACDOA010000156         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDOA010000156 DACDOA010000000
VERSION     DACDOA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.162"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..272
                     /locus_tag="IWI39_002669"
     CDS             <1..272
                     /locus_tag="IWI39_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209698.1"
                     /translation="AFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLL
                     FLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            367..1251
                     /locus_tag="IWI39_002670"
     CDS             367..1251
                     /locus_tag="IWI39_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9209699.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1473..>4041
                     /locus_tag="IWI39_002671"
     CDS             1473..>4041
                     /locus_tag="IWI39_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209700.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1318 a          690 c          922 g         1111 t
ORIGIN      
        1 ttgcatttat tattcaaggt cgtgacatat taacagtaca tcaagagttt ttcagttatg
       61 catttggtat tcctctgatc gctataaggt ctgataacaa aaagtggaag aaacgctatt
      121 acattctctt gctatttttg tgtttttatc tgccacctaa gatttttact ttattaggta
      181 tacaatgcct atcattttct tttatttcct atatcattgt tttaattttc ctcagtttca
      241 tagtatgggt attttatctt gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc
      301 acagcctaat ttttttcttt atgctatcgt tatattaaag aaatcaatca gaagggaaag
      361 aaagtgatga aaaaagtttt attagtggta aaccccagtt caggcggtga acaagcaaag
      421 gaatttgagc aattagcgat agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg
      481 catacaaaaa aagcagggga tgcaaaaaac tttactcgcg aagcggctac ggaagggtat
      541 catagtgtgt ttgtcatggg cggagatgga acagtcaacg aaggaatcag tggaatcgct
      601 gaacaagaac atcggccaaa tttcggattt ttcccattgg gtacggtgaa tgacttggct
      661 cgagcattag ggataccgct agaacctgag gaagcgatca atcatttttc gattgaatca
      721 gtcaagccat tggatattgg aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt
      781 ggctctatcc ctgaagcaat caatgatgta gatgcggaga agaagacgaa atttggaaaa
      841 ctagcttatt tcatgtcagg aatcaaacag ttagcttcca cacaatccta ctcgtttcac
      901 gtagaagttg atggaaagaa ggaagaaatt gagagcagta tcttgctgat aggactaact
      961 aactcagtag gcggttttga aacattactt ccaaatgcaa aagtggatga tggtaagctc
     1021 catttggttt atttaaaaga ttcttctttg atcgatacgc ttaaaacatt gccagaccta
     1081 ttaaaaggag tagatgaatc tacgaaaaat ctagcctatc ttacttgtga gcaattagtt
     1141 gtgtcattag ctgatgagga agaattgacg acgaatgttg atggagatga aggggataaa
     1201 ttacctgtga caatcaaagt gttaccctca catttacagg tttattgtta aaatggaggt
     1261 tgtgaccgaa gagttgtcag gaggacatcg gttgttcgga ataagggact gtgacaagac
     1321 ttttgtcaca gccttttttt gtacaaaaaa gaaattttca cctaacagaa aaatttgcca
     1381 taaaaataca tattttaaat atgaaagtac aattacaaat aaattaaaac gttaaaataa
     1441 aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atatgtaaaa aagctgattg
     1501 cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga
     1561 aaacaagcga atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag
     1621 aatcaattac ttctgaaact acagaatccc aaagtgaggc gcctacttct aatacaaggc
     1681 aagaagaaaa taagacaagt gagacctcag aagtagctga acaaacgtct gatagcagtg
     1741 aagaggtaca ggttagagtt gtttcagcgg atgggtacag cgaagctgcc acaacagaag
     1801 aactgacgca gcttctcgca gacgaatcag tagctaagat tcgtttaact cagcctttga
     1861 ctttagacag ggaattggag atcaagcgag atatcgttat cgattttggc ggacttgctc
     1921 ataatttcgg cacacatcac atctatatca atgagacacc tcagtcgatc gagtttcaga
     1981 attttaaagg aacggcagct catccaggtg ggttgatacc aaatatcgat ggaaatgcca
     2041 ttatcattgc ttatatgtcc gatttttggg gaaacagata tcattttacg ggagaaataa
     2101 aatttaccgg gacacttgac ttatatgatg gttctaaatt aggcctgatt tacgcccctc
     2161 gtgcaactgt cacattagat ggtgtttccg gtgtattgga tgtccaacca gtgaaagaag
     2221 ggatgaatgc cgctccgggt aaagcttatt tttgtagaag ctatcagcta aatgtaatca
     2281 atggtagtca gctttatgga ccatatctag gaaaattcta tggttttcta gattcaggtg
     2341 atgagacggg cagtggaaaa acagcaccag gactccatat cttgagtggt tcaaaggtct
     2401 ctcttgacta tgatcgggca gatggcgtca gcgatgaagg agaggctgtc gatacgctac
     2461 ccgggaatgt cacatttaaa gtcagtggct caggaagtga attttcggta aatacaaaga
     2521 tcaatattac caacgacaac aatcgcggaa ttatccaaat gagaggctca ggatcaagag
     2581 tcgctgtgtc gaatgatggg aaaatcacaa tcaatacagc gactactggc ggatttcgcc
     2641 tgcaaggaga ttctagtcaa atccatgtat caagtggtgg acaaatctcg gtcaaaatgg
     2701 ctggagatgg cgaccaacct ggaaacaatg ggatgcgttt tgtagggaaa ggactttctt
     2761 tgatcgtaga aggagccgga tcagtcatcg atattgataa gcaaagtggt cgttctagcg
     2821 cagttcgttt tgagaacggt acgcaaaaac taacagtaac taatggaggc tcattgaagg
     2881 taagaaatgc aggggatggc aaatcttatg tgaacagact caatcaagga aaccaagcga
     2941 tccaatttta tgacgcttct ggttttctag aaagtccagg gtcagctgat tttacggtta
     3001 ctggagaaaa atcaaatatc gatatacgcg cagattccgg agctaccgta gatacaactg
     3061 gagatattga tttgaacttt atggctggcg aaaaaactta tttagtcatg gtcgggaaaa
     3121 ctggggaaga taagactggc atcatcagtg gggatgtgtt gaatgtagac ttagaatctc
     3181 ctacctattt cgatttccaa aacaaacgga tcggaagcga gactaatggc ataggtggct
     3241 gggtatttca aggaaatggc gattcttctt tgaagatcgc tcattcagaa attgctttat
     3301 ggcgaaaggc aggagtaacg ttcgataact ttgaaaaaga tccggattat ttttctgata
     3361 tgacagactt atcgatcaat ggaaccgact taggtaattt tgtatcaagc cctagtgcag
     3421 gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa aaattataat cgaatcagtg
     3481 ccaataacca gcttccgatg attgaatcct taagagtacc aacgaatgca gataaaaaaa
     3541 tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat gcttggacag
     3601 gcgaagtaga agtgacttta cgtattactt atgcagatac gacgaaatca cctgtagaaa
     3661 ttactgctct cacacaagga caaacagatg aaaatgaagg gacaggatat aatccgtggg
     3721 gagaaggcga acaaggcggt ttgtttgagg ttcaagtacc caacgaagaa agactaacaa
     3781 cgggggatca agtgcaaata gttgctgcta aaaaagtaag aggcggccaa gaagtgacag
     3841 aactggactc tgccaaaacg acagtcgatg tgattcctcc tgagcctgct aaattaagta
     3901 ccacattgat caatgcagct tctcgctcaa tcacaggtac aggcgaagca ggagctttgg
     3961 tttcattgaa aaaaaatcaa gctgttttgt ccgaaacaac tattgatgat acaggtaaat
     4021 ttgttctttc tatcccagaa a
//
LOCUS       DACDOA010000157         3933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDOA010000157 DACDOA010000000
VERSION     DACDOA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.163"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..280
                     /locus_tag="IWI39_002672"
     CDS             <1..280
                     /locus_tag="IWI39_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9209701.1"
                     /translation="EIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQ
                     TIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            283..1179
                     /locus_tag="IWI39_002673"
     CDS             283..1179
                     /locus_tag="IWI39_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9209702.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1229..1618
                     /locus_tag="IWI39_002674"
     CDS             1229..1618
                     /locus_tag="IWI39_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9209703.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1605..>3933
                     /locus_tag="IWI39_002675"
     CDS             1605..>3933
                     /locus_tag="IWI39_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9209704.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKTRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1323 a          628 c          826 g         1156 t
ORIGIN      
        1 ggaaattccc aaaaatctaa ctgatagtgt tgacaaaatt gactttggca atggtataaa
       61 agttacagac gaaattgatg aacaccgcgg agaattgatt aagaatttag tgaccaatcg
      121 ggctaagatt caaacaataa ttaatgaaat gtcagatgaa attgatagtt atagagcaga
      181 gctgaataaa tcagattgga aagttaacta taatgaggtt tcaaagaggt atcaaagagg
      241 tatcaaaaac ttagtgaaga gttatctcaa gatgaattaa aaatgttaca aaaccttgat
      301 tctttatatc aatctttatc aaaaactgaa gagcgattgg aagaaattat tcaacaagaa
      361 ataaagctta aagattacca agttgaggtt gactcagcaa tgtcaagaat gaataaatca
      421 tatcaaaatc tctattctga gagaagtagt tcctcgcgcg aaattttttc tgatattaca
      481 ggaatagagg ctactattca accaaatagg gatttcaata attatgttaa agagctcaga
      541 gcttgtatta ataaggagga cacttttgac agtgagtttg gatctcttat tgataagctg
      601 acaaaaaatt ccattactaa tcaagatata tataatgaca tacttgatat aagagttagt
      661 ggggaaagta gtattttttc agataaaaaa ttaatcaatt ctataagaaa attgaatgat
      721 gcacaagttc ttgaattaca aacccttaag ccaaatgatt tagtagttat ccgacttgaa
      781 ctaaatggaa aaaaagttag tttatcgaat gctagtgctg gtcaaaagac atctgcaatt
      841 ctttctatga ttttggcgta tggaaattca ccactcatat tggatcagcc tgaagatgat
      901 ttggatagtc aattaattaa taacttaatc gtaagaagta tcattacaaa aaaggaaaac
      961 agacaaattt taattgtaac tcataatgca aatatcccag ttaatggtga ttcagaatgg
     1021 ttgattagca tgggagattc aaaagaaata tcggctgatc agtgcgggtc ggtagatgaa
     1081 cccaatatca agccaagaat atgtactgtg atggaaggtg gcgaggatgc ttttacaaat
     1141 agagcgaaac gctatggttt taaacaaatt gtagaataac actagagggt gagacatatt
     1201 attatgtccg ccctttttag gaggaaaaat gaaaaaaata aaaagctata ccagtatctg
     1261 ggcagttgaa aaagtcattt acgcaatcaa tgattttcag ttgccttttc ctgtgacttt
     1321 taaccaaatg gcttggttcg ttttatcttt actgtttgtc atcgtatttg cccatgtgcc
     1381 tccactatct atgatagagg aggtcttttt aaaatactta ggcataccgg tggctgtcac
     1441 ttggtttatg tcgcaaaaga cgtttgatgg caagaaacca ctgggatttt tacgatcctt
     1501 tatcagctat cacttgcgat ttaaggtgac ttttgcagga aagaaagtca aagaacagaa
     1561 gaaacgatgg gatgaaccca gtactcttgt aaggagtgtg aactatgtat ccgattaagt
     1621 atattgaaaa taatctcgtc tttaaccaag aaggcgagtg ttttgcctac tatgaattag
     1681 tgccttacaa ttactccttt ttgtcacccg aacagaaatt tcaagtgcat gataactttc
     1741 gccaactaat tgcgcaaaat cgtgaaggca agatccatgc gttacaaatt gccacagaaa
     1801 gtagtattcg cgccacacaa gaacgatcaa aaaaagaaat taccggtaga ctcaaagaag
     1861 tagcgaaaca acgaattgat atacagacag atgcattagt atccatgatt ggcgatagtc
     1921 agattgatta tcgctttttt attggcttta aactaattgc gacagatgaa gaagtcaatc
     1981 taaagagtct caagaaatct ttcttttctg ggcttcaaga atttgtctat ggagtgaatc
     2041 atcatttgat gggcgatttt gtttccttat ccaatgaaga aattcgctgc tattctaagc
     2101 ttgaaaaatt aatggaaagc aaattggctc gaagatttaa agtcaggcga gtcacaccga
     2161 gtgacctgac gtatttgatt gaacatattt atggcgaaaa agggataccg tttgaagaat
     2221 acgaatttca gctaccaaag aaaaagctaa aatcagaaac actggtgaaa cgttatgact
     2281 tattacgtcc aaatcgttgt ttaattgaag aaaaaacaag gtgcttacgg atggaacatg
     2341 agaatcatga gtcgtacgta gcgtatttaa caattaatac aattgtgggg gagatggaat
     2401 ttccttcctc agaacttttc tattaccagc aacaacaatt tacattccca atcgacacaa
     2461 gtatgaatgt agagattgtg acgaataaaa aggctttagc taccgttcgt aataagaaaa
     2521 aagaactgaa agatttagac aatcatgctt accaatctga caatgaaacg aattccaatg
     2581 tcttagatgc tttggattcc gtggatgaat tggaaaccac cttggatcag tcaaaagaat
     2641 ctatgtacaa actgagttat gtggttcgag tgagtgccga atcagtggat gaactaaagc
     2701 gacgctgtga tgaagtgctc gatttttatg atgataccaa tgtaaaacta gtccggccct
     2761 ttggcgatat gatggggcta catgaagaat tcttaccctc aagtaaacga tatatgaacg
     2821 actatattca gtatgttacg tctgattttt tggcggggct tggttttggg gctactcaga
     2881 tgctaggaga attagaaggg atttactttg gctacaatgt ggatacaggt cgtaatgttt
     2941 acctgaaacc ggcattggct tcacaaggag tcaagggttc agtaaccaat gcgctggccg
     3001 ccgctttcct gggttctctt ggtggaggta aatcctttag taacaatctc ttagtctact
     3061 atgctgtttt gttcggtggt caagctgtga ttgtcgatcc aaaaggggag cgaggagggt
     3121 ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg acgagtaagt
     3181 cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa gactcggaaa
     3241 gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac ggggagaagt
     3301 ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg gggttattac
     3361 aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc gccgatcata
     3421 tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc tcgattaccc
     3481 aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg gttttaccgg
     3541 atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt tcgatgctaa
     3601 tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc ttcaaaattg
     3661 tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg ttatcgaata
     3721 agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact caaaatagcg
     3781 acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct tttcgcagta
     3841 ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag gaggatcaaa
     3901 gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACDOA010000158         3925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDOA010000158 DACDOA010000000
VERSION     DACDOA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.164"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IWI39_002676"
     CDS             complement(573..1130)
                     /locus_tag="IWI39_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209705.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IWI39_002677"
     CDS             complement(1309..1920)
                     /locus_tag="IWI39_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209706.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IWI39_002678"
     CDS             complement(2033..2290)
                     /locus_tag="IWI39_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209707.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IWI39_002679"
     CDS             complement(2887..3165)
                     /locus_tag="IWI39_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209708.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IWI39_002680"
     CDS             complement(3199..3450)
                     /locus_tag="IWI39_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209709.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1196 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atacttttac cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACDOA010000159         3911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDOA010000159 DACDOA010000000
VERSION     DACDOA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.165"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..1529)
                     /locus_tag="IWI39_002681"
     CDS             complement(24..1529)
                     /locus_tag="IWI39_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9209710.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1526..2212)
                     /locus_tag="IWI39_002682"
     CDS             complement(1526..2212)
                     /locus_tag="IWI39_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209711.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2405..3826)
                     /gene="gndA"
                     /locus_tag="IWI39_002683"
     CDS             complement(2405..3826)
                     /gene="gndA"
                     /locus_tag="IWI39_002683"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9209712.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1080 a          837 c          676 g         1318 t
ORIGIN      
        1 aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg aataaagatt
       61 ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt atagttttca
      121 accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct agctcttgct
      181 ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg tattccttcg
      241 ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga gatatggact
      301 tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat taattgttca
      361 aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc taaagtgatc
      421 acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac ttcttttcct
      481 ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa gtctagcatc
      541 tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa tgattcttct
      601 aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc ttcaataatt
      661 gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg ttgttcaata
      721 tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc caattcatca
      781 tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat agtatccatt
      841 gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa acctaaaata
      901 ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag cttatttcga
      961 atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt ttctcttgta
     1021 cctttagaat atataggctg gatagataaa aagccagttt ttccttctag tgtaacgatc
     1081 gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt tttaaaaatc
     1141 aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag ctcagctata
     1201 aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac gacctcatct
     1261 ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag ttcttcatcc
     1321 gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt tctttctttt
     1381 gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt aaacacaacg
     1441 aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc tttcagttct
     1501 cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt ccgcgtacag
     1561 tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa cgaatatata
     1621 cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt agtaaaacgt
     1681 cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg tattctcttt
     1741 ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt tcgatagtca
     1801 aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca atatcgattc
     1861 gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt ttgacgatgt
     1921 agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct cttgcagtca
     1981 tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct aaaccgttca
     2041 attctgggag catcagatcc aacaatatgg catcccactc attatttaat gccgcatcta
     2101 aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc aactctagct
     2161 cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc atttattatt
     2221 ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat aatatgctta
     2281 aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa gtctactcat
     2341 aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa cagccgaaag
     2401 actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg acacgctcat
     2461 aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc agacgttctg
     2521 ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt acgccagctt
     2581 ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg atttcaacga
     2641 agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca tccgtaattt
     2701 tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg atttcaccaa
     2761 atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat ccttgggcat
     2821 aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat tcttttttgt
     2881 ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt acgcgttctt
     2941 ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga agtggcacac
     3001 ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca gctgcatcaa
     3061 ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc agaatgtcag
     3121 cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta aagatatcag
     3181 ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat tcagcaatca
     3241 gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt ccggctccat
     3301 ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa atttgctcta
     3361 agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt gaaggtcctt
     3421 ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg ccagagttgg
     3481 ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg tcaatcaaaa
     3541 tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt gccggtcctg
     3601 ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat tcttcgattg
     3661 tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt gttttagccc
     3721 ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag gctagatttt
     3781 tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga actcctccta
     3841 catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca ttgcaaacta
     3901 aagaacaaca t
//
LOCUS       DACDOA010000160         3900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDOA010000160 DACDOA010000000
VERSION     DACDOA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.166"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..1435
                     /locus_tag="IWI39_002684"
     CDS             86..1435
                     /locus_tag="IWI39_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9209713.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IWI39_002685"
     CDS             complement(1563..2153)
                     /locus_tag="IWI39_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9209714.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IWI39_002686"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IWI39_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9209715.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1108 a          719 c          764 g         1309 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
//
LOCUS       DACDOA010000161         3877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDOA010000161 DACDOA010000000
VERSION     DACDOA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.167"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..1021
                     /locus_tag="IWI39_002687"
     CDS             206..1021
                     /locus_tag="IWI39_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209716.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1147..1404
                     /locus_tag="IWI39_002688"
     CDS             1147..1404
                     /locus_tag="IWI39_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209717.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1656..2228)
                     /locus_tag="IWI39_002689"
     CDS             complement(1656..2228)
                     /locus_tag="IWI39_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209718.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2244..2849)
                     /locus_tag="IWI39_002690"
     CDS             complement(2244..2849)
                     /locus_tag="IWI39_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9209719.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3250..3867)
                     /locus_tag="IWI39_002691"
     CDS             complement(3250..3867)
                     /locus_tag="IWI39_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9209720.1"
                     /translation="MDTVRDFILYLKLCNRTYYFKSEKDTSSLTLTLEEGVEPDKVTS
                     KVINQLKEKGSLKHLGEYEVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMN
                     MMYISVSERTKEIGIRRALGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGS
                     LIKVNVSLDLFTILLAVGVSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1274 a          716 c          583 g         1304 t
ORIGIN      
        1 ttgtatgtag aagttttctt aatagttttt gtaataattt attaatcgta gtttatataa
       61 cttatttgta agtgactaga gaattaaata aaaaataatt actatctagt acgaaattaa
      121 ttacatagga taatatatta aggtgctgga ctacaagtaa aaaatactgt atgatcatga
      181 cagattcaag aaaggagcaa taagattggt taagatagca ggactttcta aaggtggaaa
      241 aaccgtttca caactttata aacaaaacta cagagactgg agacagttca gaaaaaacat
      301 taaggattct tactttattc taccaacaga acttgaaaaa tatttaccga ataacaaagc
      361 tattaattta tatctgtatt actgttttaa ggcaaagaat gaaggcgggg attcctggca
      421 ttcggtcaat accatagcag agtctctagg agtgacaact aaaactataa acacctggaa
      481 taaaacctta gttgatttag gtatgattgc acgtatagac gataaccatc tcagtaaatc
      541 gacaatttta cttcctctct ctagttacta taaattagcc cttgatagtt ctttaaagga
      601 tgttaccgag agcactgtcc atgaaattga tggcaacttg gttgcagttt atcatttgtt
      661 tgaatggaga aaaaatgagg ctgatgaaaa ttataacgtt ccttataaca cactctgttg
      721 tgtttttaaa agaaaaacac aaaaagatac tatctataaa tttatcttgg tccaagatga
      781 gaaaataagt aatttcgaat taagcactcc ttctagtaag atttatgacg atgcatatag
      841 atttgaatgt caagatctag aagccctcct ttccgcaaat aatctaaaca atatagaaca
      901 tgaaaacttt gttgtaaaca ctagatttaa tttagtttct gaaaaggact cagatttgtt
      961 agatttgctg atagagctta ctaaaaatct agataagaaa gaaaacatta ctgtactata
     1021 actagcatcg gtccatcatt aagtttggac tattagaaca cttgctatta cagaaaaagg
     1081 tatgctagcc acgcttacta tttattggta tcaaacaagt atcttaattt gaaaggaaga
     1141 tgatttatgg ctaatttata tatagagaac aaaggagact tgaatgccat tattacttta
     1201 atcgtagtta tggtaaaatc aataggtaat atcacaatag gaatgctgtt tattatatta
     1261 gcagcagtgc tgatttttat gatagttatt tggttcttaa atgaaagatc taaagacaat
     1321 caacaccgta gggatatgga gataaaatca atagagtcaa tagattattt atcgataata
     1381 acactaaaaa agccacgttc ctaaacgtga cttacgctga acgatatgtg ttgtttggta
     1441 gacgaacaaa tcgttcatta taaatatatg atggtatcct aatatagtaa gcgtggctag
     1501 cataccttta attactttac cttttacagt taccatacac attttttgag cagaaatcaa
     1561 gtaaataaaa aaatattata tttcacaaca tgctaataga ctcatatcta gaacgcatta
     1621 agtatttacc tgtctggttc gctgagactt cttttttaac tttcttcaat tttcttcttt
     1681 attctaaaaa gcgtactgcg actgaaaccg gttatagctt caacttcctt atacgattta
     1741 cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg tgattgttgc tttcggacgt
     1801 ccttctctga aattaggatt attctttctt gcaaacattt ttccttcttg ggtacgttcc
     1861 acaatcatat cacgttccat ttctgctaca ctcaacaacg tccgtgtcac cattcgcccc
     1921 atggacgtat tttcaatcgt tcctagattc aaaaccttga tcgttacgtt atcatcgagt
     1981 aaaggttcaa tgatctctaa tgcttctctc gtgttacgag caaatcgatc aagcttcgta
     2041 acaactattt catctccagg cttcagacca gccaacagct gattgaattt tggccgatcc
     2101 cgttttgtac ccgtaaattt ttctgaaaaa atattcttct tctctacgcc gttctcaagc
     2161 aactgttgaa tttgttcttt tgtactttga gaagcggttg agacacgtgc atatccatat
     2221 ttcgtcattt ggtccaccta cttttacaaa tcatctatcg tatactcaga atacccttca
     2281 tagaaatagc tgacatcgat tcctttcata tataactcgc gatcgtcaat tttatcagtc
     2341 agagcattgg aaattagata gcgtatttcc aaatcgttta tgggacttcg ctccattgca
     2401 gaaagatagt ctgctttatc gattaagttc caatctacaa ctttttgcaa ttcttccttt
     2461 aaaatgagat ccagccaaat tctagtactt cggccatttc cttctcgaaa tgggtgagcg
     2521 atattcatct ccacatattt cttgataatc tcttcaaaag aagtttgtgg catctgatca
     2581 atatgctgta acgaatgttc taaatacata actggtgcaa atcgaaaatt tccttttgca
     2641 atattgaccg atcggatttt tccggcaaaa tcgtaaatat ccgaaaatag aaattcatga
     2701 atttcagata aacctttaaa tgtaccaact tcgatttcat ttatttttcc acaatcgtac
     2761 aattctttcg ctttttgttt actgagtttt tcttcaacct tagccaactc aacttgattg
     2821 gttaatccca atttattctc taaaatcatg cgaaatctcc ttaactttat gacataagtt
     2881 tatgaaacac tctcaacccc attatatcgc gattttgttt atgtgtcaaa aactattaag
     2941 tttctgaccc ccgatagatt tttcaagtgt ctctattttg cacagtctag ttgacctttg
     3001 atacacctct atatttatag agaatctaaa acatcaacat ggcatactca aaattctaaa
     3061 tattagggat aaagaattct cctatacaat caaaaatttc ctttatttcg aactccggtc
     3121 acctaactct attgatctct tacaagccaa agaatatctc tatactacaa agaaacaata
     3181 gggtgcctgg ttggtctctt tgtgttttat tttagaatct agaacgtaaa tattcttgtc
     3241 attctgattt tatctaagaa tatcaattaa atcttttttt gcagcctctg aagcgggcat
     3301 tacagaaaag accagtccaa taaccgaaga taccccaacc gctagtaaaa tagtaaatag
     3361 atcaagtgac acgtttacct ttatcaaaga accaacacta taagcaagag ccattcctaa
     3421 taaatagcca attaaccctc ctgaaagagt taaaatcaaa ccttctaaaa ggaactgcag
     3481 cataatcgat tttcgagtag ctcctagggc tcttcttata ccgatctctt ttgttcgttc
     3541 agatacagat atatacatca tgttcattac acctacccct gcgataaata aagatatacc
     3601 tgctacagct gttataaaat aagtgatcgt actgaggatt tgaccaattc ctttcgttaa
     3661 catagccgta tctaacactt catattcccc caaatgtttc aaactacctt tttctttcaa
     3721 ttggtttatc actttagagg tcaccttgtc aggttcaact ccttcctcta aagttaacgt
     3781 tagagacgat gtatcctttt cagacttaaa ataataggtt ctgttgcaaa gttttaaata
     3841 aagaataaaa tcccttacgg tatctatgat ttaagct
//
LOCUS       DACDOA010000162         3816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDOA010000162 DACDOA010000000
VERSION     DACDOA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.168"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..617)
                     /locus_tag="IWI39_002692"
     CDS             complement(120..617)
                     /locus_tag="IWI39_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9209721.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(747..1457)
                     /gene="budA"
                     /locus_tag="IWI39_002693"
     CDS             complement(747..1457)
                     /gene="budA"
                     /locus_tag="IWI39_002693"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9209722.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1470..3134)
                     /gene="alsS"
                     /locus_tag="IWI39_002694"
     CDS             complement(1470..3134)
                     /gene="alsS"
                     /locus_tag="IWI39_002694"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9209723.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3339..>3816)
                     /locus_tag="IWI39_002695"
     CDS             complement(3339..>3816)
                     /locus_tag="IWI39_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9209724.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1137 a          822 c          604 g         1253 t
ORIGIN      
        1 aaatcaataa cacttgtgat tttataaaaa aataaaaaaa ccgaaaaaca gaaatgccca
       61 ctgtctttcc ggctcttttt attcttgatt tctcttcttt tttgtttcaa tagacgattt
      121 tagttattaa taaatgaaaa agcatttata ttaaaatttg ctaataaaag atctacatat
      181 ttttctagaa atgcttgatc gatttcatca taccctttcg ttactccgca atcgatatct
      241 aaaacaccga ttaaattacc ttcttttacc attggcacta cgatttctga ttgtgcggct
      301 gaatcgcagg aaatgtaatt tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt
      361 ttttcagctg attctccaca aactcctttt cccatcttga tacgtgtgca agaaactcgc
      421 ccttggaacg gtcctaaaat caattcatta ccatcgaata aataatatcc tgcaaagaca
      481 gtctcaggca aagtctcatt caacaatgca gatgaatttg ctaaatttgc taaccagtta
      541 tcttctgctt ctaataatcc ttgttgctgt aataataaaa tttcgtatgc atcttttttc
      601 tgtttttctg tccacatgat aaaaaccgct cctttttgat ctatggagaa gattatagct
      661 cagttttttt gtaaacgcta ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca
      721 ccttgaacgc atctcgctgt ctttttctat tctgcttgtt ggatttctgc tgctaaatca
      781 gcataatcaa tcgtttcgtg aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg
      841 gacgagatgc ggctaatctc aacttttcct tcagccactt caaaatccat tacatgtccg
      901 ccaaatgtat gtgtatcatc gataaaatgc aagtgaaatc ccgctgcagc aactccttgg
      961 aataattcag gcgtaaagaa cccgactaat gttccttgga cattgctttg ttggaattct
     1021 ggttgagact ctgaaatttt tgctagtctt ctgtaaggtt tttcttgctt cggcatgata
     1081 cggatgtgca tatttttgaa taatccagtt atcttcactg ccaaaaatag gttttccccg
     1141 gcttctctta ctaatatgtc ttttttcaat tgttctccat ctgcagtttt tgagacagaa
     1201 aaactagtat ctggcgtaaa atctgtaatt gctgcatagg gggtaagttc acttccatct
     1261 aattgggcaa ctgagccatc tgatcttcct tgataagcta cgccatctaa aaagatgact
     1321 tcaccatcaa gcccatgcaa tgttcccaat cccagagttc ctttttctaa tatatgagca
     1381 atcgtttctg ttccatccat caagccagcc atcaaagctc ctaatgtccc gtgctggtat
     1441 aagatttttt cagtcatgtc gacacttcct taatagaatt gatctggtaa gattgtttct
     1501 cccaatttct cattatcacg gtaatcaatc ggaatatcaa taattacggg tccttctgtc
     1561 gcaaatcctt cttctaaagc tttttctaat tcctctacac ttgtagctcg gattccttta
     1621 gcgccaaaag cttctgcata tttcacaaaa tcaactggac caaagtctac acctgacgcc
     1681 cgctgatatt tcattttttc ttggaactct accatattgt agtgtccatc attccaaatc
     1741 aaatgaacga tattcaattt tttacgtacc gccgtttcta aatcctgggc agagaataaa
     1801 aatcccccat cgccggaaac tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa
     1861 atcgcccatg gcaatgcaac accaagcgtt tgcataccat tactgaataa caaatgacga
     1921 ggttcataac ttctaaagtg gcgtgccatc caaatataat gactacctac atctacggta
     1981 acagtcatat catctgttac ttttgactga agcgtgttaa taacttctaa aggatgcaag
     2041 atacctgctt ctccttttga ttgaacaatg tcccgctccg ttaattctgc ttgaagagat
     2101 gctaaatatt cttttgcatc ttctgacact tgttgaggct ctaatgaacc tgtcaataaa
     2161 tcaagagtgg ctgagatatc accgatcaat tctctttcag gctgcataaa aggatcgatt
     2221 tcagctggcg cttcatcgat cacaatgatc cgcgcatctt tttccgcatt ccagtttctt
     2281 gcttcatatt cgattggatc atagccaata gcaataacta gatcacttcg tttcaaaagc
     2341 atatctcctg gttggtttct aaataaaccc acacgaccaa agaaatgcgc ttctaattca
     2401 cgtgagatca ctccagctgc ttggaatgtc tctacaacag gcaatgctgt ctttccaact
     2461 aattgcctga tggctaacgt ttctttttca cttgatcctc gcatacctac taatagtacc
     2521 ggtagttttg cttctctaat gcgttcagct aggtaacgga tatcatctgc agaagcagaa
     2581 cctaatttag gatcgctcaa tggcttgatg acattccctt ggacaggtgc atccactaca
     2641 tcttgcggga tactgataaa gctcgcgcct ttttttgaac tcttagccat acgataagca
     2701 ttggcaatca cttctgaaat tgtttctgga tcttggattt ctgcactgta ttttgtgatt
     2761 ggctggaaca gtgctgcatt atccatgctt tgatgggtca acttcaatag atcgcttcgt
     2821 tttacttgtc cggcaattgc taaaactggg tcaccttctg ctgtcgctgt taccaaacct
     2881 gtagctaagt tactagcacc aggaccactt gttgcgataa ctactcctgg ctctcctgta
     2941 atacgtccga ttgcttgtgc cataaatgca gcattttgtt cgtgacgagt cacgattaat
     3001 tcaggacctt gatcctctaa ttcattaaat acaccatcga tttttgcacc tggtataccg
     3061 aatacataat caacatgatg attaatcaaa ctttctacta cagatttcga accttgtttc
     3121 attttttcgt ccattgaaca tcgtcctttt tatgctttat ttttaattat tagttagaaa
     3181 taatcacaaa taattgatac agtttctaaa cctgtttcat tcaaaagtat agcacgtttt
     3241 atttcatttg tttagccata tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa
     3301 aactgtcatt cttaattgaa agtttcaatt tataaagtct acagatccat agttctcatc
     3361 aatttacgat tgttgaagta aatcaaacca aatacgatag aaggacctag tagaatacac
     3421 cattggggct ttacgaaaaa gaagagaaac aaacttgtca atagtaaaaa atctagtaaa
     3481 ctcacatacc attttttcaa cgtatagtag aacgctatcc gtagaacttg atttggtttc
     3541 gacgctggat ttctcacctg aaaataccag tagttgatgc taaaggaaac tgataaacaa
     3601 gcaagtaaaa caaacaacgg aatcaaccat tttccataag aaaaacgtat aaaaaagaaa
     3661 caatcaaaaa tcattatcac actaactatc caagcaaata gccaataaaa aaatcccttt
     3721 agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg gtgaaaaact tttttctttt
     3781 gctaattctt ctgtcatacc tatcatcacg atgcaa
//
LOCUS       DACDOA010000163         3747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDOA010000163 DACDOA010000000
VERSION     DACDOA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.169"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..785
                     /locus_tag="IWI39_002696"
     CDS             33..785
                     /locus_tag="IWI39_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9209725.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            782..1696
                     /gene="pfkB"
                     /locus_tag="IWI39_002697"
     CDS             782..1696
                     /gene="pfkB"
                     /locus_tag="IWI39_002697"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9209726.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1714..3645
                     /locus_tag="IWI39_002698"
     CDS             1714..3645
                     /locus_tag="IWI39_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9209727.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1201 a          676 c          860 g         1010 t
ORIGIN      
        1 atgctattat ttgtattgag gtgatcgtga aaatgcttac agaggaacgc agacaaaaaa
       61 tcctagactt gcttgaacag cagaaaatcg tgaaatcaca agatcttgta aaccttttga
      121 atgcttctga atcaacgata agacgtgatt tacaagagct ggagaatagt gggtttctag
      181 aaagagttca tggcggagct aagaaagagc aattattgaa ttttgagcag aatatgagtg
      241 aaaaatcact caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca gctgaaacca
      301 ttaatgacga agatgtcatt tatttagacg ctggctctac tacgttagaa atgattcctt
      361 ttctgcaagg aaaacagatc acagtagtga caaattccgt tcaacatgcg gcaaatttag
      421 ttgatttgga gcttgctacc attattttag gtggaacgat aaagctggca actaatgctg
      481 ttttaggatc taatgcgata gaacaactaa aaaattttca ttttaataaa gcatttatgg
      541 gaatgaacgg tgcccatctt gaacaaggat tcacgactcc ggatccagaa gaggcagctg
      601 ttaaacgttt agccatgaaa aattccgaag agacatttgt attgattgat cataccaaat
      661 tcaaccagtt gtccttcacc tctgttgcac ctttggaagc agccacgatc ataaccgagc
      721 gttgtccatt agaattcgtt aatgaattcc gtgaaaagac aacaattaag gaggcaatac
      781 aatgatttac acagtaacac ttaatccatc aattgattat atcgtacgcg tagatgggtt
      841 aaaactaggc gatttgaacc gaatgaagga agacttcaag ttacctggag gaaaagggat
      901 caatgtttcc cgtattttga aacggatcga ttctcaatct acagcactag ggtttttagg
      961 agggttcact ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc agtctttgtt
     1021 cacaaccgta aaagaagata caagaatcaa tatcaaacta aaatcagaca ctgaaactga
     1081 gatcaatggc ttaggcccat ctatttcaga tgaagaaatc gatgagttga agcaagcatt
     1141 aggaacagta ggacaaggag atatcgtcgt attgtcagga agcgcgccag ctagcctgcg
     1201 aaaaggattt tatcaagaac tgattgatat cattcgagaa aaaggtgcag atttcgtgat
     1261 tgatacaaca ggtgatgacc taaaaaatgc acttgttaag aaaccgttgt tgatcaaacc
     1321 aaacaatcac gaattagctg agctttacaa cacaaccttc caatctgtag aagaaatcat
     1381 tccgtatggg aaaaaattac tagctgaagg ggcacagaat gtgctgatct caatggctgg
     1441 agacggtgct ttgctcttca cggaacaagg aacttataaa acgaatgtat tagttcgccc
     1501 attgaaaaat tcagtaggtg caggggattc aatgattgct ggctttattg gggcttacag
     1561 ccgtacgaaa gatccaatcg aagcctttaa atggggagtt gcttgcggaa gcgcaacagc
     1621 tttttctgat gacttggcag ctcgcgaatt gattgatgag ttgctgccag aagttgaaat
     1681 cacaaaaatt gattagtttc aaggaggata atcatgaata tcaaggattt aatggtcaaa
     1741 gaagcgatga tcatggattt gcaagcgact gataaaaaag gcgcaatcga tgaaatggtc
     1801 caaaaaatgt ttgatgccgg ccggatttcc gatatcgaaa catataaaga aggaattcta
     1861 gcccgcgaag cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc tcacgcgaaa
     1921 aacagtgcag ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg agtagattat
     1981 gaagccttag acggacagcc aacttatctg tttttcatga tcgcagctcc agaaggcgca
     2041 aacgacacac atttgcaagc attagcagca ctatctcgat tgctgattga tgcagatttc
     2101 gtaggaaaat taaaagaagc aaatacacca gaagaagtcc aagacttgtt ccaaacagct
     2161 gaagagcaaa aagaagcaga agaaaaagca gaacaagaaa cacaagctgc agaagcttct
     2221 gctcatccag aaagaaaatt cgttgttgcc gttacagctt gtccaacagg gatcgctcac
     2281 acttacatgg cagaagatgc tttgaagaaa aaagcgaaag aaatgggtgt ggagatcaaa
     2341 gtcgaaacga acggttcaga aggaatcaaa aaccgattga ctgctgaaga catcgcccgt
     2401 gcagaaggtg taatcgttgc agcagacaaa aaagtagaga tgaatcgctt tgacggaaaa
     2461 gaactgatca atcgtcctgt cagtgatggt atccgtaaac cagaagagtt gatcaatcta
     2521 gcccttagtg gtactgcacc agtattccat ggtgatggaa aagaaagcag ttcagaagaa
     2581 ggaaatgcgg atggtactat cggacaaaga atttacaaag atttgatgaa cggcgtatca
     2641 cacatgttac ctttcgttat tggtggagga atcgctattg cgctttcttt catgatcgac
     2701 caatttattg gcgtgccaca tgatcaatta tcaaatctag ggaattacaa tcaagctgct
     2761 agctggttca accaaatcgg acaagctgca tttggtttca tgctgccggt attagcagga
     2821 tttattgctt caagtatcgg tgaccgccct ggtttgatcg ttggatttgc tgctggtgca
     2881 ttagctaata caggcggtgc aggattctta ggtgcactga ttggtggatt cttagctggt
     2941 tatgtcatcg tatttttacg taaattattc aagaacttac caaaatcatt ggatggaatt
     3001 aaaacgatcc tcttttatcc agtgtttggc ttactgatta ctggtttctt gatgttgctg
     3061 gtcaatgtcc ctatgaaagc aatcaatgat gcattgaacg gtttcttgac aagtatgagt
     3121 ggttcgaatg ctgctttact tggtgcatta ttagctggaa tgatggcggc agatttaggc
     3181 ggtccaatca acaaagccgc ttatgtgttt ggtacagcaa ctttagctac gactgttgca
     3241 actggcggaa gtgtcgtgat ggcttctgtt atggctggag gaatggtacc tccattggca
     3301 attttcgttg ctacacgttt gtttaagaat aaattcagca aaacagatca agatgctggt
     3361 ttgacgaaca tcgtgatggg actatcgttt gtcacggaag gatcgattcc atttgcagca
     3421 gctgacccga ttcgtgccat tccaagtttt attattggtt ctgctttaac tggcggatta
     3481 gtcggagctt tcggaatcaa attactggct cctcacggcg ggattttcgt tgtattctta
     3541 ctgagtcacc cggtcatgta tttggtgttt attgctatcg gagcagtcgt atctggtgtg
     3601 atttatggtt tattgagaaa agctcctgca gtagcagaag catagaaata aaaaagaaga
     3661 ggctgggttt tccggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctga
     3721 gtcagaatga accacattct ggctcag
//
LOCUS       DACDOA010000164         3709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDOA010000164 DACDOA010000000
VERSION     DACDOA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.170"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2657)
                     /locus_tag="IWI39_002699"
     CDS             complement(<1..2657)
                     /locus_tag="IWI39_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209728.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPIGYELDATPIEFEIERSQTAVV"
     gene            complement(3329..3597)
                     /locus_tag="IWI39_002700"
                     /pseudo
     CDS             complement(3329..3597)
                     /locus_tag="IWI39_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          977 a          853 c          612 g         1267 t
ORIGIN      
        1 accaccgctg tctgacttct ttcgatctca aactcgattg gtgtcgcatc taattcatac
       61 cctattggcg cttgagtttc taccagctga tatgctcctg gtgctaaacc gtcaattgcc
      121 agttttccat cttctcctgt agttagaccg gtttgtagcg tttctccttc tcggttctgt
      181 aactcaaaaa cagctccttg caggatttcc ccgctttggt catcgatctt tgtcaacacg
      241 acaccgcctg gtgtaagtcg gttttctttc gtcaattcta ccaccgctgt ctgacttctt
      301 tcgatctcaa actcgattgg tgtcgcatct aattcatacc ctattggcgc ttgagtttct
      361 accagctgat atgctcctgg tgctaaaccg tcaattgcca gttttccatc ttctcctgta
      421 gttaaaccgg tttgtagcgt ttctccttct cggttctgta actcaaaaac agctccttga
      481 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
      541 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      601 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      661 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      721 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      781 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      841 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      901 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      961 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
     1021 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
     1081 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
     1141 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
     1201 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
     1261 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
     1321 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
     1381 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
     1441 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1501 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1561 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1621 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1681 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1741 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1801 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1861 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1921 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1981 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     2041 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     2101 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     2161 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     2221 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     2281 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     2341 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     2401 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     2461 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2521 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2581 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2641 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2701 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2761 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2821 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2881 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2941 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     3001 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     3061 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     3121 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     3181 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     3241 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     3301 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     3361 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     3421 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3481 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3541 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3601 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3661 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttc
//
LOCUS       DACDOA010000165         3698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDOA010000165 DACDOA010000000
VERSION     DACDOA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.171"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            368..1168
                     /locus_tag="IWI39_002701"
     CDS             368..1168
                     /locus_tag="IWI39_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP9209729.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            1184..2062
                     /locus_tag="IWI39_002702"
     CDS             1184..2062
                     /locus_tag="IWI39_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9209730.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            2075..3055
                     /locus_tag="IWI39_002703"
     CDS             2075..3055
                     /locus_tag="IWI39_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9209731.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1292 a          584 c          756 g         1066 t
ORIGIN      
        1 ttatttaaat attttgaagg caaaaaagga caagaccata aagaggagaa tcaaaaaaag
       61 aagtctaaga aaagaagtga gtatattttt tagaaagaat aaaactggaa cgtaaagatt
      121 tacaagtaat cagtaacccg taccttttat atttaaagag gttgtttttc tataaatcgt
      181 tgtaaagaat gactaagttg tattattttt tcacaaacat tcatattata atgtttcttt
      241 ttaataaaaa gattgttttt aagctaatta ttatatgtgt gttattgaac taactcttga
      301 cagataatca aaaagaattt atagttgtta ccgtagtagc tactaaaaat aaagtgagga
      361 ttaatcaatg gaaacgatta ttcaaatcat gaaaaaatat gatttttcag agatggagac
      421 aagagtgtat accacactat tagaaaaagg taatcttaca ggatatgaag taagtaaaat
      481 ctcaggtgtg ccacgctcga aagtatataa tattttggaa aaactattaa aaaagaattt
      541 gattgtcgtt aataaatcag aacctaaact ttatcatgcc atctcggcaa atgagttttt
      601 agagaaatta gaaaaaagtg ttaaaaatga tctttctttt cttaccaaga atctagggat
      661 gatcaaagaa aaagatgaag aagaaatgct gtggaaagtt gatggtatcg agtatgttct
      721 ggataaagta gagcatttgg tgaaaaacgc aaaagaaagt ttgctcattc aagtttggca
      781 tgagaatttc acggattcac tactaaaggc attgcagcaa gctgaaaaaa gagttgataa
      841 gtttgtactg attttattca gttctaccca tgaatatgat cttcccttag aaaagtatta
      901 tattcatggt tttgaaacag acaaacttgc tgactttgga gcaagatgga tcaacatcgt
      961 cgccgatgag caagaagtcg tgtttggtac cattaacgaa gaactgcaat caaccgatgt
     1021 cacttggaca aaaaaccacg ccatggtcaa tctagcaaaa gaatatgtca aacatgatgc
     1081 atatacacta aaagtgattg cagaatctcc tgaagaactt aaagcaaaat atggtgaggg
     1141 ctttgaggga atcagaaaga tttactagta ggaggaattt tttatgattc ggaatatttt
     1201 gctgacacta attgtcgcaa tcaatctata ttttattatt tactttctaa gagacctttt
     1261 caaaaacaaa caaaatttca aagaagaacc aggggatatg cgtctgttgc ccttcacgtc
     1321 atttatcatc ttctttcttt caacatttgg cgtttccgac tttgctatcg gaaccgtcct
     1381 ttatccaaag ttgaaatggg tcagcatgaa aaaactaccc ggaacgttga atacccaatg
     1441 tgtagtaccg gtagccgtca tggcattgtc ttacatcact gccatcaacg tagggatcaa
     1501 gacactagct gtttgtatta tctgccaaat tatcggtgca tatttaggac caagatttgt
     1561 cgtgaagtta ccagagaaaa cgatcaaact gtttgtcggc ataggattgg tcattgctgc
     1621 attgttgatt tttgcaggac aaatgaactg gattccatca aacggaacag ccagtgaatt
     1681 gtatggcgga aaattaatct tggcaggatt tttactcttt gtctatggtg cattgaacaa
     1741 tatcggcatt ggttcttatg cgttaacgat ggtaactgtc tacttgctag gcttgaatcc
     1801 agttgcagcc tttcctatta tgatgggggc ttgtaccttc tcagtaccga tcggcagtgt
     1861 tcaattcatt aaatttaatg aatacagcag aaaaatcaca ctttttactt caaccttcgg
     1921 tgtattgggg gtattggtcg ctgtattcct agtcaaatct ttagatacct atatcctgaa
     1981 atggatcgtg attttggtct tactatacag tgcatatacg atgttgggca gtcaattaaa
     2041 aaaagttaca gcagctaatt aaggagcgat tcaaatgtta tttcttaaaa aagaagatat
     2101 tatcaaaagt ttttcaatga gagaagccat cgatgcggac aaaaaagcac tgtcattgta
     2161 ttcagcagga aaggcaagtg tgcctcttcg caccaatatc gatgtgccaa aaagcaacgg
     2221 acaaagctta tatatgcctg cttatgtaga aggtggcgaa ggcgcgctag gtgtcaaaat
     2281 cgtttccgtg tatcctgaaa acatcaagaa aaacttacca agtgtaccag caacgatgat
     2341 cgtattggat ccagaaacgg gtatggtatc cgcttgtttg gatggtactt atttgacaca
     2401 attaagaaca ggcgctgttc aaggagcagc aacagaactt cttgcaagag aagatgctaa
     2461 aattggcgcg ctgattggaa ccggcggaca agcacagtca caattagaag caatgttgac
     2521 cgttcgtaaa ttagaagaag ttcggatttt tgatattgat tttgagcgtg ccagccaatt
     2581 tgctgaaaaa atgatgcaac aattttctgt aacgatgcgc ccaacaaaaa ccagtcaaga
     2641 atgcgttgaa ggagcagaca tcattacaag tgtgaccact tcaaaacgtg cgacattctc
     2701 tgctgagtgg gtcaaaaaag gtgcgcacat caatggagtc ggtgcttaca caccggaaat
     2761 gtgtgaaatt ccacgagaaa taatcaaagc cgcagatgtt gttatttttg ataccatgga
     2821 cggtgttcta aaagaagctg gggacttcat ttcaccgctg caagatgggt atattcaaag
     2881 agacagctat catggggaac taggccaact gatcaatgaa gaattgatcg gtcggacaag
     2941 tggagaacaa atcacgatct tcaaaacagt tggttcggct gtattagatg tggtagttgc
     3001 gacagaaatc gttaagaaag ctaaagaaaa taatttggga aaattgattt tctaattaaa
     3061 aaggctgaaa ttaaaacctt tattattgcg tttaaaaaaa gaaaacaaag aagtttgtaa
     3121 tcaaatggaa actgacccca taatttaaat aaaataaaaa acatctcttg ttgaattcta
     3181 gtaatgaagc gaccctaaaa cttagaccaa aaaatctaac ttttgggtgg agttgtcaag
     3241 aaaaaccggg cacttagtta aaaaataagt tggacgggtt atactaaaat tgacaccaaa
     3301 ttaagagaaa gcgaagatct ggattatgcc aagacgttca tttgatgaaa tatttaaaat
     3361 taccgctgta aaactggtaa cggaagaagg gttttctgtt aaagaagtta gtctatagct
     3421 agaagctcgc tctaatagcg tgtattgatg agttcaagaa gttgaaaaat atggataacg
     3481 tacttttctc ggtagaggaa gtcatgaaat ccattcgcag aaatgaaatt acacgcttag
     3541 aaaaagagaa taaacggtta aaagaatagg tagaactcct aaaaattacc gaagtcttcc
     3601 tgaaagaaaa ggctcttcgt caaattgtgt tggcgaagac aaatttgaat aaaacttagt
     3661 ccgatatgcg gcgcgttctg tgcgccgcat atttttta
//
LOCUS       DACDOA010000166         3590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDOA010000166 DACDOA010000000
VERSION     DACDOA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.172"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(284..961)
                     /gene="nth"
                     /locus_tag="IWI39_002704"
     CDS             complement(284..961)
                     /gene="nth"
                     /locus_tag="IWI39_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9209732.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(968..1666)
                     /locus_tag="IWI39_002705"
     CDS             complement(968..1666)
                     /locus_tag="IWI39_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9209733.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1934..3118
                     /locus_tag="IWI39_002706"
     CDS             1934..3118
                     /locus_tag="IWI39_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9209734.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3124..3567)
                     /locus_tag="IWI39_002707"
     CDS             complement(3124..3567)
                     /locus_tag="IWI39_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9209735.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          918 a          721 c          603 g         1348 t
ORIGIN      
        1 tcatttttat tacaccttta gatagatacg tcacttattt atataatcct ttggaatact
       61 ggacttattt tataggtata ttggaaggca ttattttcta catcgggtat tttcccaata
      121 aaaataacaa cttttatttt tatcgcaata aattgtaata agttaccatc ctagctactg
      181 agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt ctccataata
      241 acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg cgtggtttct
      301 tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat attgatagca
      361 aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca aaaaaaatca
      421 gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt gtttcttcta
      481 cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca cgttccacgt
      541 gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca tttgctgtct
      601 ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact tgcccatcga
      661 aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt cgatacaaac
      721 caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac gcatcgggtg
      781 tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct gtcgcctggg
      841 cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt tctccatgtg
      901 cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt ttgcttaaca
      961 tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct tcttgttgag
     1021 ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg tcttcttcca
     1081 cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg tcaacgtaat
     1141 taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa atttccggct
     1201 gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc
     1261 gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct tgtgtttttt
     1321 cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag atggggtaaa
     1381 gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg ataaatccat
     1441 ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc atatccatag
     1501 caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc aactgtagaa
     1561 caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt aaaagattag
     1621 atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt tctcctccgc
     1681 tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt ttctatttac
     1741 ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt catgtttttg
     1801 atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt ccttagaatg
     1861 ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca ttattgcata
     1921 aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg gatacgtctc
     1981 tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt ttttaacgct
     2041 gccttattct cactccatac atcagcaagc tttctatatc actttgctca tgtctgttta
     2101 tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc attttgggcg
     2161 tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc tttttggttt
     2221 aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga cggcaggaga
     2281 aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa gaaccaaagt
     2341 ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta ttataggggg
     2401 tctattagct gagttgggga attctgttcc tatatttatt ggagcactct ttactttctt
     2461 aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa aacgttcagt
     2521 agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat tcaagatttc
     2581 atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg gctctcttca
     2641 atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt tggttggttt
     2701 aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt
     2761 gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg aaatcctcgc
     2821 ctatctattc atcacattat ctggaattct tctactgccg attttgtttt tatttgggat
     2881 tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc aactatccaa
     2941 ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta tacaatctct
     3001 atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac catttcttcc
     3061 gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata aaaaatagta
     3121 gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa atactgtgtc
     3181 aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc atttttgatg
     3241 gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa aatagatttc
     3301 ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca gtttttttgt
     3361 tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg tcacgttagg
     3421 atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa atcctgcaaa
     3481 ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat ccagatattc
     3541 ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca
//
LOCUS       DACDOA010000167         3551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDOA010000167 DACDOA010000000
VERSION     DACDOA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.173"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(860..1201)
                     /locus_tag="IWI39_002708"
     CDS             complement(860..1201)
                     /locus_tag="IWI39_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9209736.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1201..1431)
                     /locus_tag="IWI39_002709"
     CDS             complement(1201..1431)
                     /locus_tag="IWI39_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9209737.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1718..2056
                     /locus_tag="IWI39_002710"
     CDS             1718..2056
                     /locus_tag="IWI39_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209738.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2261..2500)
                     /locus_tag="IWI39_002711"
     CDS             complement(2261..2500)
                     /locus_tag="IWI39_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9209739.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2476..2823)
                     /locus_tag="IWI39_002712"
     CDS             complement(2476..2823)
                     /locus_tag="IWI39_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9209740.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1119 a          668 c          548 g         1216 t
ORIGIN      
        1 ttttactcat tcatagacac ttccttaaat aacttttatt atagaaggta ttgtaaataa
       61 taatattgac aaaaagtgac tcaaaataaa aaaaacctta cattatactg gaatgaactg
      121 tattgatagt gtacctaaaa gctctactga cataagatcg tgtaatcggt aaagtaattt
      181 tattaattat acgggatgta atcaattaga gaataaacta atcataaaag gtacttttac
      241 attcataatt gtcgctttga gattctactt tggttcacta tctgacccaa ttgcacattt
      301 tcgatcatta caagtattgg tgtcgttaga acaactaaat aaaaactagc ggtagatttt
      361 tcagaatcta tgctaatttc tgtatggatg ttccagtgat ggcaaatttc tcttttcatt
      421 gtttttcttg tcgcatgata tgcctttttg tgttattcag ttaaactata gaaattcttt
      481 cctagacaga tcttgatccc aaattttacg tttttgagca tcgatatagt ttaaaatgac
      541 attactcata tctaaagagt ttagattttt taggactaaa actgtcagtt gttctttttc
      601 ttctttctgt taaagtattg tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa
      661 caagttttgt gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc attcgtttaa
      721 aatgctatat attctttagt ttaagatacg gaaattaatg ccatcgacca cactagtaga
      781 atgaacgaca tttacttggc ctttttttaa tttcgttatg tttagcatat aaaaatatta
      841 tagagtttac ttacaatgct taaaacatat actgtataat ctgatcaatt ttagccatgt
      901 cttgcaatgg taagttttct atttttttga gttttcgttc cttaaaatca agagatttta
      961 attgtgtaat aattacttgt ccttttatat ctagctcttc aggtaatgag taacgtgtcg
     1021 gtaaattctt tatagtggaa gtaattggac atatgaccga aaacattgtt ttacggttaa
     1081 attcataacg agagacgact agtcctggtc gtcttttttg aatttctttt cctacggagg
     1141 gatcaaaatc aatccataca atatcacctt ttttaggaat ataaattctt tcttctgaca
     1201 ctataaaatt tctctccctt caggagttaa atcttcccat tcgtctttct cataatactc
     1261 ggcctcttcc ccaccactaa atggatcttc aatttttgga actaacgtaa ttgttccatc
     1321 atcagaatac acaacgatat attcttggtt ttctgaaggt ttctttccgt tatcagaagg
     1381 taaagttacc actacagaac ttccttgtaa tctagatttt gtaacaagca ttatcatcac
     1441 tcccttaata aaagtatact atataacact aagtaacgca aaattcccct ttgtaaacta
     1501 tctagaaaat tgttttgttt aaagtatatc ttattggata taaatagaaa taaattgtag
     1561 ccagttcatt ttttctttaa ctaaaaaaat aatccaagaa acattgaacc tatggatcca
     1621 aaagctaccc aaatactata gctaaccccc cgctaccttc aattttgcag ataaaacgct
     1681 ttgggagaaa atttcaaaga caacaggcca tcctatcatg cccgattata aacaaaaagc
     1741 tggctattcc ttttgctctg acaaatttca attgggaaag ttcacgaaac tagcaggtga
     1801 agaagtttca actgttcgga tcaaagaatg cccgcttcaa atagaaacat tggttcaacg
     1861 aatttcaact agacaagact ttgctattgt agaacgtgag atcgtagcta tttttgtaga
     1921 agaagggatt ttacatgatc caactcatat caatgtggat aaatggcaac ctcttatcta
     1981 taaattcaga gaatatacaa ctgccaacca gtcgttaact ttgaatttta gattccaaga
     2041 attcaaagag tcataatgat aaacatcgtt aggtataaat aaaaaagact cgcagcacga
     2101 gtaaaaaaca aatagaagct atacattaga aaggtcgact gtgaccctat agatgcagta
     2161 tatgcagctt atgagcattt cttctgttct ctttgttttt gggatctgtg ctgaagtctt
     2221 tttcgttttt tgccgctctt tctttgaaaa atcgattatg ttataccttc tccattacta
     2281 attcaggcat ctgctgagta atgatttttc gcgcaaaaga aatatctgtt acccaaggtt
     2341 cgatttgttc ttcagtcaag acaagcggca ttcggtcatg gattggtgcg actgtgcggt
     2401 taggttcagt agtcattatg attgattctg ccattttttc atcctgatgg ctacgataaa
     2461 aaccgccaat atacactatt ttttccttat ttgaaaacat aaatttgtct ttttcttcat
     2521 tccactcatt gggaaaacac aacgattttg caaaaatggt tgatgaaagg tctttttttc
     2581 ttctactgtc tctgcacggg cattaatcat caattgtcct tttttaaatc cggtaaatcc
     2641 ccacttcatg atttccggag tgaggatgtt ttcttcctct tttcttaacg tcacaacaag
     2701 atttgacgga aaaacttctc cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg
     2761 ggcttctcga taataattct gaaggagctc tgactctaga tcaaaaaaat aacgaccaca
     2821 catcctaatc cctcccaatc tttttttcta tcatacactg caacaagaag ttcgcaccaa
     2881 caaaacgctt tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt
     2941 gaatggtatt ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa
     3001 gctgcttttt gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg
     3061 gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga
     3121 aagctatttt cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct
     3181 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
     3241 tgagcagcta tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac
     3301 ctctccacta aacggtgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa
     3361 tatgaggaac tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca
     3421 acctctaata cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa
     3481 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc
     3541 acaacctcta a
//
LOCUS       DACDOA010000168         3476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDOA010000168 DACDOA010000000
VERSION     DACDOA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.174"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..2857
                     /locus_tag="IWI39_002713"
     CDS             188..2857
                     /locus_tag="IWI39_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9209741.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2948..3388)
                     /locus_tag="IWI39_002714"
                     /pseudo
     CDS             complement(2948..3388)
                     /locus_tag="IWI39_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1013 a          639 c          656 g         1168 t
ORIGIN      
        1 aaatttttaa gggtgggact tttgattcct accctttttt tgcgtttatt tttgttatac
       61 gaacaaatta tgaagttatt tattctaaat tttatttttt tgtgaaaatt agactctttt
      121 taagcagaga aaaacaagga tatgataaac tgaatacaac ttcatagaaa tttggtggaa
      181 aataaatatg aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc
      241 tagtttcctg attccgtttt tgattttagc acttgtttat ctaagtatcg ggatctatcc
      301 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
      361 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
      421 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
      481 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
      541 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
      601 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
      661 catcatgtgg ctggatgcat ttgtttatct tccattagtg attttgggga ttcatcgtgt
      721 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
      781 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
      841 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
      901 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
      961 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
     1021 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
     1081 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
     1141 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagtttct atcttgttcc
     1201 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
     1261 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     1321 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     1381 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     1441 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     1501 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     1561 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     1621 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     1681 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     1741 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     1801 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctaatgga
     1861 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     1921 cttccgccaa gctggtcaaa gtggcgacta tacactgtat cagaatgtca atgcattgcc
     1981 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     2041 gaccagtcag acaaacttga tcaatacatt gtctggttta aatgaacagt attttacatt
     2101 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     2161 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     2221 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcacaat tagaaagttc
     2281 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     2341 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     2401 tggaacgaaa gaagtaagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     2461 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     2521 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     2581 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     2641 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     2701 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     2761 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaaa
     2821 accgcgaaat acacacagaa gaagacgaag aaaataaaga aacgatggaa tctgtgtcac
     2881 tcttctattt aaatggcaaa aatttcaatc tttaaatgac aaaaaccacc tgatttttgg
     2941 tggtttttta tatacctatg actaaactat ctcttgctgc ttcgacaact tctacagtga
     3001 tcttatccat ttgattgatt cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa
     3061 gcctaaaatt tccttttgtg atcttaatga tagttgtgat agcctcataa tcagaaaaat
     3121 cttctagctt gatatcaatt cctaaatctg cccatttgta ttccaaaata tgatgtgttt
     3181 cgtccttact aaggttatca aattcatgtg caaatcctat gcgagagtaa agctgcggat
     3241 aacgagacaa tcttttttca ataccaggca taccgataag aaccattgcc agatcatttc
     3301 tatcgtaaat atctcttagc tgttctaaat gctgaagctt taaacgatca atttcatcga
     3361 caataattaa gtcaattgct tcataggttt taggcacagg aggatcttct tgattgtatt
     3421 ttagtaccat atgcattgct ttagtcagac ctattctact agttattgtg ttaata
//
LOCUS       DACDOA010000169         3434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDOA010000169 DACDOA010000000
VERSION     DACDOA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.175"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            253..465
                     /locus_tag="IWI39_002715"
     CDS             253..465
                     /locus_tag="IWI39_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209742.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(743..2542)
                     /locus_tag="IWI39_002716"
     CDS             complement(743..2542)
                     /locus_tag="IWI39_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9209743.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2666..3262)
                     /locus_tag="IWI39_002717"
     CDS             complement(2666..3262)
                     /locus_tag="IWI39_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9209744.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1260 a          617 c          597 g          960 t
ORIGIN      
        1 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
       61 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      121 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      181 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      241 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      301 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      361 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
      421 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
      481 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
      541 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
      601 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
      661 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
      721 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
      781 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
      841 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
      901 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
      961 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1021 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1081 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1141 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1201 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1261 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1321 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1381 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     1441 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     1501 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     1561 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     1621 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     1681 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     1741 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     1801 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     1861 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     1921 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     1981 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2041 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2101 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2161 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2221 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2281 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2341 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     2401 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     2461 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     2521 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     2581 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     2641 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     2701 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     2761 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     2821 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     2881 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     2941 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3001 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3061 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3121 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3181 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3241 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3301 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3361 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     3421 aataacttgt tttt
//
LOCUS       DACDOA010000170         3336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDOA010000170 DACDOA010000000
VERSION     DACDOA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.176"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..287
                     /locus_tag="IWI39_002718"
     CDS             <1..287
                     /locus_tag="IWI39_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209745.1"
                     /translation="VNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAK
                     NHIHKSLTYISEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            642..878
                     /locus_tag="IWI39_002719"
     CDS             642..878
                     /locus_tag="IWI39_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9209746.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            875..1288
                     /locus_tag="IWI39_002720"
     CDS             875..1288
                     /locus_tag="IWI39_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9209747.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            1807..2757
                     /locus_tag="IWI39_002721"
     CDS             1807..2757
                     /locus_tag="IWI39_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAP9209748.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(2754..>3336)
                     /locus_tag="IWI39_002722"
     CDS             complement(2754..>3336)
                     /locus_tag="IWI39_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209749.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1220 a          469 c          589 g         1058 t
ORIGIN      
        1 aagtaaataa aattctggag tcggaaaatt ctggttacag attaagcgaa agcaatgaat
       61 ttattccaat cacagatgat atttctatta agtctataaa tgctgtatct gaatcaccat
      121 ttaattacgc aaaaaatcat atccataaat ctcttacata tataagcgag aaggaaaatc
      181 aagattataa tactgtagtg agagaagcaa ttagtgctgt cgaaagttgt cttatcgaat
      241 tttcaggatt accagctaat aaaaaaatac tttgggaact gctataaaag caattaaaga
      301 caaatcgaat aacaatgtcg atcttacatt tttaaaacca tttgagacaa tgtatggttt
      361 ggcgagtaat aatggaatac gacatgcaga aaatgaaaaa acaattgtct cagatttacc
      421 tgatgcagtt ttggtgttaa caacatgttc agccgtaatt aattatttgg gagtaaaaat
      481 agcatatgac tccaataaat catgaaaagt taaaattcat ccaagagcta cattataact
      541 attaatttcc aactctttaa aagtttagta attgaaggaa gtaataaggg tgattgaaca
      601 atacggatat acggtgtata cttatcgtag gagttgatga tatgaatatt aaaaaacgca
      661 aaatcagaga agtaggtaat tcaatagttg taactttgtc aaaagagagt ttattgcaaa
      721 aaggtttgaa acctggagat actattttta ttgatgaaga taaaatgatg gaagctattg
      781 taaaagagga aagcaagata gatcttgaaa ttgatatgta tgttaaccaa gctttatctg
      841 agtatgatgc agctttcaag gaattagttg aaaaatgatc tatctaagtg aaaaagatat
      901 tgtaaaattg aatgttattc aaataaagaa atattcaccg cgagaaacaa tcggagtcat
      961 tgacaaaaat gcgctgagta tggctgtaaa tcaacctaaa cagcatattt ttggaaaaga
     1021 tctgtatcct gataaattta gcaaagcagc tgttctgcta ataaatctga tcaaaaagca
     1081 tccatttcat aatgctaaca aacgtacagc atttttagcc gcgtatgttt ttctcaagct
     1141 aaatggctat tcactaaaaa tgaaaaatca ggaagtagta gaatttgtag ttgaaattgc
     1201 tacgtatcag ggtgattttg atgacttaaa agaggcaaca acagatatac taaaaaagaa
     1261 ttcaacaaag accgattcaa aaaagtagaa gcaaactagg gattcagtta aatctgaaga
     1321 tttagtttgt tctttatttt agtttacata agatacatta tgcgaagttc attattttaa
     1381 aagcttaaat aataaacttt tttataatac caatttattt atgtaaacca atgaccttaa
     1441 tcgcttaagc aagcatgtta tgtaatactc tactctctcc aaaactaatg actatctatt
     1501 ttcaaagaag ctagaatgat tatctgatca aatgataatt tatatttaat tcctggtcta
     1561 ctgctcttct tttaatcaat taatgtctaa cgtactgtac acgataaagt aggaagactt
     1621 attttcaact tcggattcat ttttttaatt cggtgaaaat cactaaatta agagtttatc
     1681 caagttcatt ttagctgaga atacgcttga aaataatttt gatgaatttt cctgaattaa
     1741 aatttattaa cttgttgagt gatataattg taatgtatta ccaaatagtt agcacttaga
     1801 ttagtgatgg aaaggaatag tgagaacaag atgactggta gaaatcccaa agtatttata
     1861 tcatattcat gggagaataa ggaaaataaa gaatgggcta gagaactagc agacaaatta
     1921 cttgaaaacg gtattgatgc atatatagat cagtatgatt taagtttagg tgatagatta
     1981 cctcagttta tggagcagca aatatccgat tcagattatg ttctaattat atgtacacca
     2041 gagtataaaa aaaaggcaaa taatcgtaca agtggtgttg ggtatgaagg tcatattatc
     2101 tcagcagaat tgctttctgg aaaaaatgaa cgtaaattta tcccaataat taaagaagga
     2161 cgtgcaagcg aagtacttcc tacttttctt gctggaaagc ttggaattga tttatcagat
     2221 agccagcatt atgatgaaaa cttagatgat ttaattacaa ctatatttgg tgttaagaga
     2281 aaaccagcat taggtgatat acctaggcat attaaaggca gtcctttact acctatagaa
     2341 cataatgaaa ataaagatat tgaaatctta ggaattatca ctgataaagt aactgtacct
     2401 aaaatggatg gctcaagggg ttctgcatta tataaaattc catttagatt aaatagagta
     2461 ccaagtcagc tttggaaaga gttgtttgtt agaaattgga attcaccacc acgatataca
     2521 acaatgcata gaccaggaat tgcttctgtt gtagacgatg aaattttact taatggaaca
     2581 actattgagg aagtccggga atatcatcgg gaaactttaa aactttgcat tgaaaaaagt
     2641 aatactcaag aaaaaaaaat attgctagag gaggaacaaa aaaaagagag agagaatgag
     2701 cgagtcagat cacataaaga aagtgttcag aaattagcag atgaattaga gttctagttg
     2761 atagtcagtt ctttcttaat tcgatacaca gtttgtctag ttacattata gtctgttgct
     2821 atttttgaaa tagcaacacc attctcctgg tcctgaacga tgcttctata aacaaggcga
     2881 cgttgaggat cttttgtgtt cgcactgtat aacttaggac gtcctttata gacgccgttt
     2941 gctttagcaa tctttatccc ttgtgcctgg cgtcgttttg attcagtacg ctcttgttca
     3001 gcaatcatgg ccaagatttg aatgattagg tctttgataa atttatccag gagaggattt
     3061 ccgattgctt cagccatgat cggtaaatta gtgataataa gtcgcacatt tttcttcttt
     3121 agataattta ctgaatcgat aatctcgtca taattacgtc caagtcgatc gatagcctct
     3181 acaataaaaa tatcttggtc tctaacaaaa tctagaactt cctgaaaaat cggtcgatga
     3241 gtaatagtcg ctccagattg tttctcgatg aaaatttttt cagcaccaaa ttttctcatt
     3301 tcctcgattt gacgttgttc attttgatcg atcgat
//
LOCUS       DACDOA010000171         3296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDOA010000171 DACDOA010000000
VERSION     DACDOA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.177"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..906
                     /locus_tag="IWI39_002723"
     CDS             <1..906
                     /locus_tag="IWI39_002723"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9209750.1"
                     /translation="DSSSLLLLSFSIILPYLIVDLKLCNRTLVTMVDSNGKPILNKYG
                     KPKKVVKMPIDNSVTLSIQRLRHLASLGNESNKQIVDRYFSNQPEPLTINEQMELLQT
                     GINLHSIYMVDENENEIITAPAIARWVINKHTLEVTARINPEYLEWFSEVVNQYILFN
                     LRDFVKLKSKYSMILFLELSTFWNTGMYYKRINDLREILGVNPTRNNRDFINNVLKPA
                     IAALKEYFPELSLTVEKNSSDSRKITHVRLKFDKLTITDMQERLRSSEGASLVEYQQE
                     LENMEGEEKAVNDPTPEIDLSNVEW"
     gene            1020..1388
                     /locus_tag="IWI39_002724"
     CDS             1020..1388
                     /locus_tag="IWI39_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209751.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAP
                     KAPDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKN
                     SCLNNRKKPVTVMAMGFLCI"
     gene            1562..1870
                     /locus_tag="IWI39_002725"
     CDS             1562..1870
                     /locus_tag="IWI39_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209752.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNN
                     KQEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN
                     "
     gene            2133..2453
                     /locus_tag="IWI39_002726"
     CDS             2133..2453
                     /locus_tag="IWI39_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209753.1"
                     /translation="MERFLTKNRKGGGKLEKKEDLLNAFKNLDEKDQLKALLRLHDIT
                     YSDIAERLTVKKGEREGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQND
                     QNKA"
     gene            2584..2793
                     /locus_tag="IWI39_002727"
     CDS             2584..2793
                     /locus_tag="IWI39_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209754.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHN
                     FKELENTAPAPSQGNQESEGMPHAW"
     gene            2783..3055
                     /locus_tag="IWI39_002728"
     CDS             2783..3055
                     /locus_tag="IWI39_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209755.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELI
                     GVCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
     gene            3103..>3296
                     /locus_tag="IWI39_002729"
     CDS             3103..>3296
                     /locus_tag="IWI39_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209756.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDTT"
BASE COUNT         1234 a          595 c          656 g          811 t
ORIGIN      
        1 gattcatcgt cattactcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac ccttgtaact atggtagata gtaacggcaa gcctatctta
      121 aacaaatatg gaaagccgaa aaaggtagta aaaatgccga tagataactc ggttactttg
      181 agtattcagc gcctacggca cctagcaagc ttaggaaacg aaagcaacaa acaaatagtt
      241 gatcgttact tttcaaacca gccagaacct ctgacaatta acgagcaaat ggaattactc
      301 caaacgggga taaatttaca ctctatttat atggtagatg aaaatgaaaa tgagatcata
      361 accgcgcccg ctatagcaag gtgggtaata aataagcata cgctagaagt aacagcgcgg
      421 atcaatccag aatatttaga gtggttttca gaagtggtta accaatacat tttatttaat
      481 ctaagagact ttgtgaagtt aaaaagtaag tactccatga tccttttctt agagctttct
      541 accttttgga atacaggaat gtattataaa aggattaatg atttaagaga aattttagga
      601 gtcaatccaa cgagaaataa cagagatttt ataaacaacg ttctaaagcc tgctatcgct
      661 gctctcaaag agtactttcc agagctaagc ctaacagtgg aaaagaattc tagcgactcc
      721 agaaagatca ctcatgtaag attgaagttt gataagctaa caataaccga tatgcaagaa
      781 cgcctaagaa gcagcgaggg agcttcttta gtagagtacc aacaagagtt ggaaaatatg
      841 gaaggagagg aaaaagctgt caacgatcca actccagaga tcgacctttc caacgtggaa
      901 tggtaacacc tgtcagcaat ctaatccaat aaaccaaagt acttgtaagg cgcgagggct
      961 ccttttagag ccttctagtc ctataccaaa ccaagcaaaa taaaggagct ataaccaaca
     1021 tggaaaacac taaccaaaca ccaaatacag caccaactaa ccaaactagc ccagccgaac
     1081 gcaaagccga tcttattaga ttgtactcac gccttgaagg tatgaaactc caacacgttc
     1141 cgaccgctcc aaaggcgcca gacggcttcc caaaatcggc agagagcctt gagtttttcg
     1201 agtgtaaggt gtgcggatcg acttttcaaa cgattttaag ggaatccgtt gcgagtggct
     1261 gccccaaatg tgaacatgat cgcttaggaa ttttagccga tcatttacca gaaccagaca
     1321 aaaacagttg tttaaataat cgaaaaaaac ctgtaaccgt tatggctatg gggtttttgt
     1381 gtatttgaga taatttccaa gggttattta tggatttatt ttaaaaaatc tattaaatta
     1441 tactaccact ggtagtaata taaatatatc gaaagggtac gagggttaaa gcgtaatggc
     1501 ggaatgagta ccgttctctt ttgaaaatac caaaccaacc aattaaagga gagaaagtaa
     1561 tatgaaagaa atgaaaatga agcaagtaat ttatcccgtg attattggag ttttagctat
     1621 cgctcttatt atccttggtg taaattgcta taacttaagc aaggaaaata atcaacttga
     1681 gaccaaaaac aacaagcaag aaatccaaat agccaagcaa gcttacgtta tcagcgatta
     1741 caagacttat tttcgtgaaa actatgtaga caccgtgaaa tatcgctccc acttcactga
     1801 taaacaaatt ttaaatgttt atcgagaagc tcttagagat ggaaaaatca gcgatgaggg
     1861 agctaactag tggaagagct cattttgctg gctatgttat tacaggaaaa gaagcgtgaa
     1921 cgagaagcct aacagctgga aagaatccga ataggatatg aggaagcaga tcgggaaaga
     1981 gatcagaaaa tagaggagct ggagaagcgt atccaagaac tttccaaaaa ctatgataat
     2041 aaccaagatt gggaacaaga gtggcggaaa atcgttggta taaaggaaaa tgaatactta
     2101 gtgagaaagc cgccagaaga agaggaagac ccttggagcg atttctaact aaaaatcgga
     2161 agggaggtgg taagttggag aaaaaggaag atttattgaa cgcttttaag aacctagacg
     2221 agaaagatca acttaaagcg ttattaaggc ttcatgatat aacatacagc gatattgctg
     2281 aaagattaac cgttaaaaaa ggcgagaggg aaggacaaaa aggtgttagc ctcgtgactg
     2341 tacaaaaagc tttccaatca aagaccaact tggagagtga tcgctggaaa gagattgtaa
     2401 aagtcgctac caagctaatt gaagagcgac aaaacgacca gaacaaagca taaaaaaagc
     2461 ccgtccattt accgctgacc aaagcaataa aatgaacgag ccacaccatt tacttttagg
     2521 ggtaaccaca cgccctaaaa gctaccaaac catttaccaa ataaccacta aggagattta
     2581 gaaatgatta gaaaacttaa taatatcaag ctttctttgg caattttaac actatttttt
     2641 ggaatttaca ataattccag aatggctttg ttttttctta cgttattgat attcatgaac
     2701 ttaattatcc ataatttcaa ggagttagaa aatacagctc cagcgccctc tcaaggaaac
     2761 caagaaagtg agggcatgcc acatgcttgg tagttttaaa aaggataagg aagtaaaaac
     2821 acgaggagcc cttacaccaa gccagcaaaa taagcttggt acaaaagcca ctttccaccc
     2881 cgtccgagat caagccaacc aaaaagaact aattggcgtt tgtgtagggt ttacttcaga
     2941 gaaatttttt ccaataatag agtgcaaaat acacgggatc acaactagag aagttgctag
     3001 aaaagttgtg aaatggcaca ccagccaaac caaaaccaac cataagaaac gttaaaaacg
     3061 ttcctaagag cgtttgaaaa ttaaattaag ggagatttaa gaatgaacaa caaaaacacc
     3121 aaaaatacca atactaacca cgagaagaga agcttctttg ataagctttt caaaaagaag
     3181 aagaatggaa aagtatctat tttgaaaatg atcggctgga tatttttagc ctttttcata
     3241 gttgctgtga acagtagcgg cggtgataag aaagaggctg cttcaaaaga taccac
//
LOCUS       DACDOA010000172         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDOA010000172 DACDOA010000000
VERSION     DACDOA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.178"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IWI39_002730"
     CDS             301..585
                     /locus_tag="IWI39_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209757.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IWI39_002731"
     CDS             588..1004
                     /locus_tag="IWI39_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209758.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IWI39_002732"
     CDS             complement(1141..1860)
                     /locus_tag="IWI39_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9209759.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="IWI39_002733"
     CDS             2535..2630
                     /locus_tag="IWI39_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9209760.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="IWI39_002734"
     CDS             complement(2932..3177)
                     /locus_tag="IWI39_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209761.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DACDOA010000173         3155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDOA010000173 DACDOA010000000
VERSION     DACDOA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.179"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..478
                     /locus_tag="IWI39_002735"
     CDS             212..478
                     /locus_tag="IWI39_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9209762.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            505..804
                     /locus_tag="IWI39_002736"
     CDS             505..804
                     /locus_tag="IWI39_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9209763.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            978..1529
                     /locus_tag="IWI39_002737"
     CDS             978..1529
                     /locus_tag="IWI39_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9209764.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1675..1968
                     /locus_tag="IWI39_002738"
     CDS             1675..1968
                     /locus_tag="IWI39_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209765.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1961..2257
                     /locus_tag="IWI39_002739"
     CDS             1961..2257
                     /locus_tag="IWI39_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209766.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2332..>3155
                     /locus_tag="IWI39_002740"
     CDS             2332..>3155
                     /locus_tag="IWI39_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9209767.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLIST"
BASE COUNT         1180 a          438 c          628 g          909 t
ORIGIN      
        1 agatttagaa aaacttacta aacaaagaga tatgaaaaac tttaataatt gtgtagtttt
       61 gataattata actgaattaa ctttatttac aaatatatat tatatccatg aagcccaatc
      121 ttctttaata gaagattggg ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa
      181 taaaggataa cgttatgtca caaagggagt aatggacagt ttgccggtgg aattacaagc
      241 tttatgttgg aatctaatcg atcaaaacgt tcaaaagcaa tttcctttag actatcttca
      301 aatttttgaa ttttctacgg aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc
      361 ggaagaaaga aaagagtatc tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa
      421 aatatgggcc attgattcgg gtgagtatca aacgatgtta ttaccagaag aatactagta
      481 tctaaaagaa aggaacgaaa ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt
      541 gcacagctgg tcactgcttc tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag
      601 gaaattgaaa tgggtatgct tcctgaagat gttcatctac cacgtttagt gatgacacat
      661 ctttatattt attgcgcacc agaagatgga aaagactatg tagtttattt cattacagat
      721 attaccagcc agcgagaatt tgttcgtggg ttactggtag aaggacgatt ggtttggagt
      781 cagattggag gaacggatga ataagatcga ttatttgaga atgattgcga aatgtttaga
      841 agatatcctt accgcgattc atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat
      901 tttcaactag tattgacact tataacggca atggtaattg aagtgcttga acaaaaaata
      961 gcataggaga aaaagagatg attgaaaaag aattagtgac cacacaaaca gaagtttggc
     1021 gagatcctca tcatacccat cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag
     1081 aagcggaaga taaattagca gtagtcatta ctattcgtcc aacagataca gaaccattta
     1141 cgaatgattt gagtatgtta ttaatcgaaa agaatatccg acaattagga tttactggtt
     1201 ttattgctgt gaatcttttt tcgtacacaa aagcgaaaag tcctcgggta tttcttcgag
     1261 gaaatgatga acaatctttg gaaatattaa cgactgtttt tacagaaaag aaaatcaata
     1321 ccattatttt tgcttgtggt tcgattacag cgacgagtca agtggcttat gaacaagcaa
     1381 agactattta cgatcaattg agtgcgaaac aaaagaagat gaccaaagta ttgataaatg
     1441 cagaaggtaa gttatcccac ccactaagta ttcatgttcg taatgaatgg caattggcag
     1501 atagtcaatt attatttcaa gaaaaataac attttatttt ctcatgttac ttataagtag
     1561 ttttacatgc tttttgactt taaaaataca tgtgatgata catgttatat gaattatcaa
     1621 tgtacccagt aatatgctat tttcattatg taaaaaataa cagggggtgt gtagatggag
     1681 attgatgaaa cttgtttgag taactcttat aaaagtatga atgacttttt tgaggaaatg
     1741 gaactggatt cagaattgct ctatcaattt tatcttgctt ttcgagggca acagatttct
     1801 tttccaatga aaatgtacga tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa
     1861 caggaaaaaa ctgtagatat ccgtcaattg acagaagttt atggtttctc tgcaagatgg
     1921 attcaaaaag taattaaaca aaaagaaagg agacgagtac atggctaagc tgattgtatt
     1981 aggtggttta atttacggtg gatattggtt atatcaatat atgcttgcgc atccgaatat
     2041 agataaagga aaagtaatta agacagttgg ggtaatcata gttggactca tagctctaac
     2101 taagaaaaat aatactaata gcccaaaaat aatgagaact tatcgctgta gaggatgtgg
     2161 aagggaaatt tctactagtt ttaatccatc tgagagtcct caaggaagag accgctgcca
     2221 tacgggctat cgacattatt gggaaagaat cgattaaaag attaccattt agatcaatta
     2281 attgtctgat agaataagta cgtgagggag aaattgaaag ggggaaatat aatgaaaaaa
     2341 tggctgttat tactaatagg aatgggaatt ttattcactg gttgtacatc tgagaatacg
     2401 aggactaaag cgactataga aactgataca acaattgaga ctaaaaaaag taaaacgcaa
     2461 tcaactgaac aaactacaac agaaatgaag caatctgtgg aaacaagtgc aagttcaatc
     2521 actgaatctt cttctgaaga ggttaagaat actttgtggg atacgaataa agcttccaaa
     2581 ttagaaactt ttgtcacaca atggggaaag acattaggtc aagaatataa aagctacaat
     2641 cttcagaata atgtaagtct gtatggcact cctttaccac aagccgtgat taatggcgat
     2701 tggaaaatgg caatcaatga agcaccagtt accgtacaat ggtcagaaga tggaactggt
     2761 catgcagatt tcaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg
     2821 cagcatgttt actttttcgg ttttcaaaat ggacaaccta aagtttattt aacgcaacaa
     2881 aatcaaggta atgaaaataa ttatctgtac tttaacgaaa ctcaaaatca acagttaaaa
     2941 caaggtttca gtgatattgt gaatggacaa actcctcaaa caccagtagt agaagaatct
     3001 actcaacaag caacaactac aacgacacaa tctacaagta gttcaggaat ttcaagttgg
     3061 gaagctgcta aagaatctgt gctaaataat aaagctttat ggttgtatga ttctcctgat
     3121 aaaaatgtat caaatactaa tttaattagc acaaa
//
LOCUS       DACDOA010000174         3155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDOA010000174 DACDOA010000000
VERSION     DACDOA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.180"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(456..1766)
                     /locus_tag="IWI39_002741"
     CDS             complement(456..1766)
                     /locus_tag="IWI39_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9209768.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3115)
                     /locus_tag="IWI39_002742"
     CDS             complement(1778..3115)
                     /locus_tag="IWI39_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9209769.1"
                     /translation="MFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLFLISRILD
                     AFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMTVKIAYIF
                     VSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGFFIPILMY
                     THNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSASQLVKGL
                     LANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGTVVVLAPF
                     ATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSGLFNLMVW
                     AFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQSSTTGGA
                     IQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKINER"
BASE COUNT          974 a          649 c          496 g         1036 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttct
//
LOCUS       DACDOA010000175         3129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDOA010000175 DACDOA010000000
VERSION     DACDOA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.181"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..618
                     /locus_tag="IWI39_002743"
     CDS             13..618
                     /locus_tag="IWI39_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209770.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(684..1316)
                     /locus_tag="IWI39_002744"
                     /pseudo
     CDS             complement(684..1316)
                     /locus_tag="IWI39_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            1605..1997
                     /gene="merR"
                     /locus_tag="IWI39_002745"
     CDS             1605..1997
                     /gene="merR"
                     /locus_tag="IWI39_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HAP9209771.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKQLHSCPIIETLT"
     gene            2026..>3129
                     /locus_tag="IWI39_002746"
     CDS             2026..>3129
                     /locus_tag="IWI39_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9209772.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKMGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIADANYHPGEAEEFQSEQKTNLLKKYRLNVEGMTCTGCEEHIAVA
                     LENAGAKGIEVDFRRGEALFELPYDVDIDIAKTAITDAQYQPGEAEEIQVQSEKRTDV
                     SLNDEGNYDYDYIIIGSGGAAFSSAIEAVTLNAKVAMIERGTVGGTCVNVGCVPSKTL
                     LRAGEINHLAKNNPFVGLHTSASNVDLAPLVKQKNDLVTEMRNEKYVNLIDDYGFELI
                     KGEAKFVNENTVEVNGNQITAKRFLIATGASSTAPNIPGLDEVDYLTSTSLLELKKVP
                     NRLTVIGSGYIGMELGQLFHNLGSEVTLIQRSER"
BASE COUNT         1086 a          494 c          605 g          944 t
ORIGIN      
        1 aaggagtttt ttatgggaaa aattggctat attcgagtat cttcacaaga tcaaaatttg
       61 gatcgacaat tggcaatgat ggaaggactt aacattgata aattattcca agaaaaagcc
      121 agtggtaaag atactgagcg tcctgagttt caaaaattac taaattatat ccgtgaaggt
      181 gattgcgtca tcgttacatc actagatcgt ttaggaagag attatgaaga tataaaaaat
      241 acagttgctt ttatgaaaca gaaaaaagta gcgttaaaaa tattagatgc acagttttta
      301 gattttaata ctggaaatga actcctggat accgctatgt tcgatatgtt tttatcactg
      361 ctaagttata ttgcacaaaa cgagcgtgag aaaattcgtg aacgtcagcg gcaaggagta
      421 ttattggctc aaaaagccgg ccgatataag ggacgaccaa tcgagtattc tattaactca
      481 acagatccgc aaaaacgctt agtttttaaa accgtggtgg acatgttaaa gcaaggcatt
      541 ccagttgcag agattgcaaa agaaaacggg atctctcgac caactattta taaaataaag
      601 aaagcgaatg atttgtagaa atatactaca ttcattcgct tttctaccgc actatttcgt
      661 ttctatgttc catgtacccc gatatagggt ccattataaa tcacactatc agagttcgta
      721 ccatatctat ctccttcttt ttcaacaact tcctgttttt gaggaaaaaa tgctgtatta
      781 attagcatag aagtgaaata tgggattggc gtactcaaat taataaccaa agtattctta
      841 tccttagcat aaactcccaa cttatctggt ttcatagttc ctgagattat ttctttacca
      901 tttttaatag attcaaaaaa accaatattt ggtgaatctt gattacttaa cgttcttttc
      961 cacgaataaa catagtcatc tgcaacaaca ggcgtaccat tactccacat tgcatccttt
     1021 ctcaatcgaa taacgtactt aagtccatcc ttagagattt ctggaaggcc attagctcct
     1081 ccaggtacaa tttcaccttt agcattaagt ttatataatc cttccattac gttatttaaa
     1141 acaagttggc ttgtagggtc gatagctaac ttcggatcta agctttgtac tgtagtagaa
     1201 acagcaaaag tataagcttt ggtttctttt gctgtttctt gagacttttc cccacaacca
     1261 gtaataatta ttcctaacat aagaattgtt aaattctttc cccaagttct tttcaaaaaa
     1321 acacccctat tcaatttctt atctgattgt tctagcaaca cttagcttaa ttatactgta
     1381 aaaaaaacgg gtcttcggaa tatatagtgg aattactttc ttaatagttt aaaaacaact
     1441 ggtgtttaaa tcggattttc tggtgggatg ttaggataat caatagtatt atataaaagt
     1501 atcaatcaca tacgagtgat actttgggaa aaaaataaaa tagtccaatt tacttgaacg
     1561 tgtactatgg tacacggttt ataatattca tggggtgaaa taagatgatt tatcgcatta
     1621 gtgagtttgc agataaatgt ggagttaata aagaaacgat cagatattac gagcgaaaaa
     1681 atttattaca agaacctcac cgaacggaag ctggttatcg gatatattca tatgatgacg
     1741 ttaagcgtgt tgggtttatt aaacgaatac aggaacttgg tttttcttta agcgagattt
     1801 ataaattact tggtgttgta gataaagatg aagttcgttg tcaagatatg ttcgaatttg
     1861 tttctaaaaa acaaaaggaa gtgcaaaaac aaatagagga tttaaaacga attgaaacta
     1921 tgttagacga cttaaaacaa cgatgtccag atgaaaagca attacattcg tgtccaataa
     1981 tagaaacatt aacatgagag attaattaac gaaaggagct tttttatgaa taaatttaag
     2041 gtaaacattt caggaatgac ttgtacgggt tgtgaaaaac acgtagaatc agcacttgaa
     2101 aagatgggtg ctaaaaatat tgagtctagt tatcgtcgtg gtgaagcagt atttgaactg
     2161 cccgatgata ttgaggttga aagtgcaata aaggcgattg ctgacgcaaa ctatcacccg
     2221 ggcgaagcag aagaatttca atcggaacaa aagacgaatt tattgaaaaa atatcggcta
     2281 aacgttgaag gaatgacctg cactggttgt gaagaacata ttgcggttgc tcttgaaaat
     2341 gcaggtgcaa aagggattga agtagacttt cgtcgcggag aagcactgtt tgaactaccg
     2401 tatgatgtag acattgatat cgcgaaaaca gcgattactg acgcacaata tcaaccgggc
     2461 gaagcagaag aaatacaagt gcaatcggaa aaaaggacag atgtaagttt aaatgatgaa
     2521 ggtaactatg attatgatta catcatcatc ggttctggtg gagctgcctt ttcatctgcc
     2581 attgaagccg ttactttgaa cgcaaaagtg gctatgattg agcgtggaac ggtgggtgga
     2641 acttgcgtta atgtcggatg cgttccttct aagaccttat taagagcagg ggaaatcaat
     2701 catctagcaa aaaataatcc atttgtggga ttacacactt cggcttcaaa tgttgattta
     2761 gcgccattag taaaacaaaa gaatgattta gtaaccgaga tgcgaaatga aaaatatgtg
     2821 aatttaattg atgattatgg ttttgaatta ataaaaggtg aagcaaaatt cgtaaatgaa
     2881 aatacagttg aagtaaatgg caatcaaatc acagccaaaa gatttttaat agctacaggt
     2941 gcttcttcaa ctgcacctaa tattcccgga ttagatgaag tagattattt aacaagcact
     3001 agcttattgg aattaaagaa ggttccaaat cgtcttaccg taattggttc aggatatatc
     3061 ggcatggaat taggacaact atttcataac ctcgggtcag aagtcacttt gattcaaaga
     3121 agcgagcgt
//
LOCUS       DACDOA010000176         3001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDOA010000176 DACDOA010000000
VERSION     DACDOA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.182"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..963
                     /locus_tag="IWI39_002747"
     CDS             4..963
                     /locus_tag="IWI39_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9209773.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="IWI39_002748"
     tRNA            1152..1225
                     /locus_tag="IWI39_002748"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1740..2039)
                     /locus_tag="IWI39_002749"
     CDS             complement(1740..2039)
                     /locus_tag="IWI39_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209774.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2045..2275)
                     /locus_tag="IWI39_002750"
     CDS             complement(2045..2275)
                     /locus_tag="IWI39_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209775.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2525..2725)
                     /locus_tag="IWI39_002751"
     CDS             complement(2525..2725)
                     /locus_tag="IWI39_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209776.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT         1019 a          509 c          519 g          954 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
     1861 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
     1921 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     1981 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
     2041 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
     2101 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
     2161 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
     2221 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
     2281 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
     2341 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
     2401 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
     2461 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
     2521 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
     2581 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
     2641 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
     2701 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
     2761 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
     2821 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
     2881 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
     2941 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
     3001 t
//
LOCUS       DACDOA010000177         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDOA010000177 DACDOA010000000
VERSION     DACDOA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.183"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            36..2096
                     /locus_tag="IWI39_002752"
     CDS             36..2096
                     /locus_tag="IWI39_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209777.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2179..>2732
                     /locus_tag="IWI39_002753"
     CDS             2179..>2732
                     /locus_tag="IWI39_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209778.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVL"
BASE COUNT          977 a          500 c          539 g          716 t
ORIGIN      
        1 catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac
       61 aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg
      121 ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac
      181 ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg
      241 attcccctat cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg
      301 cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc
      361 ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg
      421 atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg
      481 ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt
      541 attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg
      601 atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt
      661 caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct
      721 attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg
      781 atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta
      841 aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca
      901 aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata
      961 tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg
     1021 ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag
     1081 cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt
     1141 ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta
     1201 tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg
     1261 aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta
     1321 cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa
     1381 acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg
     1441 taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga
     1501 ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag
     1561 aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag
     1621 aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt
     1681 ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa
     1741 tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat
     1801 tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta
     1861 aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt
     1921 caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa
     1981 cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg
     2041 caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta
     2101 tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag
     2161 acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca
     2221 agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt
     2281 tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa
     2341 acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt
     2401 gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct
     2461 tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca
     2521 gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct
     2581 accctccata cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt
     2641 gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat
     2701 ccagattatg aaaaagttat gcaggtttta ag
//
LOCUS       DACDOA010000178         2594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDOA010000178 DACDOA010000000
VERSION     DACDOA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.184"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..243)
                     /locus_tag="IWI39_002754"
     CDS             complement(<1..243)
                     /locus_tag="IWI39_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209779.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAI"
     gene            376..750
                     /locus_tag="IWI39_002755"
     CDS             376..750
                     /locus_tag="IWI39_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAP9209780.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            804..>2594
                     /locus_tag="IWI39_002756"
     CDS             804..>2594
                     /locus_tag="IWI39_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209781.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQK"
BASE COUNT          985 a          367 c          313 g          929 t
ORIGIN      
        1 aatagctgtc aataccagta aattattttt tttatttttt gaattatctt catttaaaat
       61 agaatttatg ttagtactat aaaattcaca aatttttttt agtgtctcta tatctggtgt
      121 tgttttgtta ttttcccact ttgaaatagt ttgacgtgta acgttaaatt ttcttgccac
      181 atattcttga gtataccctt ttttcactct taattctctt agcttagatc ctaaatcctc
      241 cattttttct cctatgtaaa cgattattta cataaaattt tatcataaaa atactaacgc
      301 gccaaaaaag ttctttattt tataaaacac actaaaataa aataattcta gtaaaaaaga
      361 taggagaaat aagttatgaa aaaattttta tgtctttctg ctttaatggg tgtcttatta
      421 actagtggag gaatagctag tgccacagaa gctttaaatc tagatgtacc cgaagaccat
      481 accgccattt acggaggagg aatggaagct acagaagaag gattttcttc tagtaaattg
      541 agatatgctg ctggcggcgg ggatttcaac tgtggggtta atggatttaa agtttatgca
      601 aattactacc atgctagagc taaacatagt gcaacagcta aaaatggacg aggcggtcaa
      661 gtacgatcag ttcagaaagc tggagtaaga gcttatgcaa catgcaatgc cacacttact
      721 ggaaatactg gttggtggaa tgtttattaa aaaagagagt ttaaactctc ttttttattt
      781 acatttgaaa ggaaattcaa aatatgttaa aaaaaataaa cgctgtttta tcactttttg
      841 tctgcattac aatactattt ttttctacta atgttataaa tggtataaag acttctgaag
      901 cgctatataa aaataccgct gaaattcata tgcaatataa agatgatatg gactttaaca
      961 gttatataaa cgctttgtat gaaatatcta cacaagaaaa tattaatatt tctcaatata
     1021 gttttactgc tgttaaccaa ctaactattc ttgctacaaa ccctaacgct aacaaacact
     1081 ggaaattttc taaaaaaaat atattagcaa gaaacgattc taaagtccat tatactaata
     1141 acaaaaattc taagtaccaa ttaaaacttc caaataattt tttaaatata aatatttatc
     1201 ctttttctca tgtggaaaat attggattaa gtgaaatatt atatgtacaa ggaaactcaa
     1261 acaaattaat tcctgtactg aaaaaatatg gaacacctac tatttccaat atcaatcaat
     1321 cagatacttt tcaaattaat actaatattg ttcttgttat ctgctatctt agtatattta
     1381 ttgtaattac tacaattata tttgcttttt caaaaatgaa agaaatcact ctaaaaaaaa
     1441 tgttaggcta taactctttt gatactataa aagattctat taaagagtta aacctcttat
     1501 tttgttcttt gattataact ttcattttat ctattttttt gagcatatat tacagtaacg
     1561 aaaatttttt cctatcattt cttctatgta gtatttttca ttttataatt agttttttat
     1621 ttatcatagt tactctaagt atctatcatt tatctagtat aaaaaattat ctaaaaaatt
     1681 cacgaccact taagttaaat ctttgtatat taaatatctg tctattctta agcatgatcc
     1741 ttctgttaat agcttctaca aaagtaataa atacccataa tgaagccgaa aataattctc
     1801 ttaaatattg ggaaagaact actaatttat acaaaacaaa tattactaat caattaaata
     1861 gaaacaacac cgtagaagaa aataactact tgaaaaaagc tagtaaattt gtttataaaa
     1921 tacagaagaa ttataaaact tttattatcg cgccatacaa ttacgcaact atccaagaga
     1981 ataataaaga attttttatt ggacaaaaag tttatccaaa ttttgaagac tatgttagtc
     2041 aacctgctgg tgcaggaata tgtgttgatt taaactatct aaaatattac aatcctattt
     2101 cttttgaaga atctactgac ttaaatttaa taaactctga tccactaact acttacattc
     2161 tagtacctaa aaaatataaa gaatatgcaa aaatgattga aaaaaattat ttagaagaca
     2221 tacagtttag acttgaggac tctcctcctc aagctcctta taaaaatcct aatttgaaaa
     2281 atttaaaaat aaaactaata tttgttaata ataaccaaaa gtattttagt tttaatacct
     2341 tatatggaaa ggcaaaagat aactatacaa taactgatcc catagttaga gttattaatc
     2401 cagaaataac agaatcatta ttttggggaa atatattgac tagtgatggt ggattatttt
     2461 ttgatcagaa acatacttca aaccaaaact tttttaatca aattaatccg tatataaaag
     2521 aatttaacct agaaaatatc ataaattttt cttattctgt ttttcaagaa actggtgaat
     2581 tattatctca aaaa
//
LOCUS       DACDOA010000179         2564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDOA010000179 DACDOA010000000
VERSION     DACDOA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.185"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..480
                     /locus_tag="IWI39_002757"
     CDS             <1..480
                     /locus_tag="IWI39_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9209782.1"
                     /translation="PLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIER
                     SYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            492..1496
                     /gene="neuB"
                     /locus_tag="IWI39_002758"
     CDS             492..1496
                     /gene="neuB"
                     /locus_tag="IWI39_002758"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9209783.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1493..>2564
                     /gene="neuC"
                     /locus_tag="IWI39_002759"
     CDS             1493..>2564
                     /gene="neuC"
                     /locus_tag="IWI39_002759"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9209784.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSR"
BASE COUNT          967 a          334 c          482 g          781 t
ORIGIN      
        1 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
       61 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
      121 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
      181 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
      241 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
      301 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
      361 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
      421 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
      481 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
      541 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
      601 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
      661 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
      721 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
      781 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
      841 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
      901 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
      961 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     1021 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     1081 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     1141 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     1201 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     1261 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     1321 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     1381 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     1441 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     1501 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     1561 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     1621 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     1681 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     1741 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     1801 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     1861 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     1921 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     1981 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     2041 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     2101 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
     2161 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
     2221 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
     2281 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
     2341 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
     2401 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
     2461 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
     2521 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaa
//
LOCUS       DACDOA010000180         2479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDOA010000180 DACDOA010000000
VERSION     DACDOA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.186"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..399
                     /locus_tag="IWI39_002760"
     CDS             <1..399
                     /locus_tag="IWI39_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9209785.1"
                     /translation="ADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSL
                     GSAFRKLQSVGLYTKTEHRTVKYMNNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETI
                     RGIYKKNRRNGTLFGFSVSTEIKVLMGIPA"
     gene            482..1099
                     /locus_tag="IWI39_002761"
     CDS             482..1099
                     /locus_tag="IWI39_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209786.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1263..1595
                     /locus_tag="IWI39_002762"
     CDS             1263..1595
                     /locus_tag="IWI39_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209787.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1919..2080
                     /locus_tag="IWI39_002763"
     CDS             1919..2080
                     /locus_tag="IWI39_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209788.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            2112..2479
                     /locus_tag="IWI39_002764"
                     /pseudo
     CDS             2112..2479
                     /locus_tag="IWI39_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          873 a          345 c          504 g          757 t
ORIGIN      
        1 gcggacggct taaccttaga tatctggtta cgaaagaaac gggatacgca agcagcctat
       61 gctttcttaa aacgactcca taaacagttt ggtgagccga aagcaattgt gaccgataaa
      121 gcaccttctc ttggctccgc ctttagaaag ttacagagtg tgggtttata tactaagaca
      181 gagcaccgaa ctgtgaagta tatgaacaat ttaatagaac aagaccatcg acctattaag
      241 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
      301 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      361 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      421 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      481 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      541 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      601 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      661 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      721 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      781 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      841 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      901 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      961 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
     1021 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
     1081 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
     1141 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
     1201 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1261 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1321 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1381 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1441 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1501 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1561 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1621 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1681 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1741 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1801 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1861 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1921 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1981 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     2041 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     2101 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     2161 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     2221 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2281 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2341 gaaacctata tcaaaattaa gggacattgg cattatcttt atcgtgcaat tgatgcggac
     2401 ggcttaacct tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc
     2461 ttaaaacgac tccataaac
//
LOCUS       DACDOA010000181         2474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDOA010000181 DACDOA010000000
VERSION     DACDOA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.187"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            580..1539
                     /locus_tag="IWI39_002765"
     CDS             580..1539
                     /locus_tag="IWI39_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9209789.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1526..2131)
                     /locus_tag="IWI39_002766"
     CDS             complement(1526..2131)
                     /locus_tag="IWI39_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209790.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2371..>2474)
                     /locus_tag="IWI39_002767"
     CDS             complement(2371..>2474)
                     /locus_tag="IWI39_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209791.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          824 a          406 c          384 g          860 t
ORIGIN      
        1 acaataaatg aagacttttt tattgttaat tgtttatttt tctattatta gatatgaaaa
       61 tcgcatttag ataaaataga aaaatatgaa tatattttaa atattaagat gagagtcatt
      121 aacttatgat agtagtcaat ttgcttttca ctaattacaa aatatgtgat ataggaagtt
      181 tagttattgt tctcataatg ccatcctgtt tttagattat ctagtaattt ttttgttatt
      241 taaaattagg agcagtgcca attgttttaa ttttaaaggc tttttttata tcttctaaat
      301 tagaagatat ttgtatatca atatggaata tattaccttt tttgtatttt tttatgttat
      361 ctttagctaa aacatcaaat acatgtcctt gaaattgtat cgaggcagct ggatttagtt
      421 taaattgagg aaactttacc acagctgatt caaaacactt gtcacatttt tttatttttt
      481 taccttcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
      541 aaatagttcc gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      601 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta
      661 gcccatctgg tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct
      721 ggtggaagcg caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga
      781 cgtccgattg tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg
      841 tggactccag atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca
      901 ctatatcgga tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa
      961 agaaaaccaa acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag
     1021 gatagaacaa ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc
     1081 attgtcggtg gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg
     1141 atcatcacct tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa
     1201 tggtttcaat ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag
     1261 ttctcgaatt ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca
     1321 ggaacacctt ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc
     1381 ttatataaag ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca
     1441 aaacgaaatc atattcctag aaaatcatta cactatcgca caccattgga agtatttttg
     1501 agttacgtag gtaaagatga tttgtctagc ttaatttgac aaacgaaact ttgtaaaaat
     1561 attcgattga atattctatt tggcacgact cacttggcag tatatgtatg tcaatatttt
     1621 tcatctgttt cataggtatt tcaaaatcta aactattaaa aacatataat tccctgtaat
     1681 tatttacatc taactctact aaatttatct ttggaacaat agatcctcca cccattccac
     1741 cataataaaa aaaatcttgt tctctatcaa aatagcacat attaagctca gtttgtttat
     1801 catcctcaat aattttataa atcaaaccta tataatcatg aatgctggca aaaatttttt
     1861 ttaagtcaag ttctaaatct tcttcgaaaa cacatttaat ttctcctact gataattgat
     1921 actctatatt acatgactta attagttttt tagatccccc ctccagatag gcatatgttt
     1981 caataattat gttcttttta ttgcttggca aatttccgtt atttattgca taaattaaaa
     2041 atgactgtct atttagccca taatctaata caacttcttc atactcatat ttctttaaat
     2101 atttaatatg aaaatatgct gaattaatca aaactcgagt gttactgttg ttatttattg
     2161 ttgttctatt gtgtataaaa ttgtataggt tgtcatattt tcttggttta gtgaactact
     2221 caccacttag ctaaacctaa cggtttctta cgtttgaagt gggagcttct tgggaagaga
     2281 gcatacttga tagcgtatta ctttactaac agaggtgcct cttatttacc aagctatccc
     2341 cgtagttcct acggttcttg tttatttgat ctaagccaat cccatagagt gtagccttag
     2401 accttcggtc aggatattga tactagcgtt gatatctcgg tcatgatgag tatgacaaat
     2461 agggcaagtc catt
//
LOCUS       DACDOA010000182         2463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDOA010000182 DACDOA010000000
VERSION     DACDOA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.188"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..1150
                     /locus_tag="IWI39_002768"
     CDS             62..1150
                     /locus_tag="IWI39_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209792.1"
                     /translation="MYSKNWKKKLVMGVMVGSIFVFAGCSASTDKSKTATSETKTAEV
                     VKSKMKQIEKNVEKILAEKDKDTKLSEYKKFVTESSAYSKSDKTEKKITNTYTESIKK
                     IKTNFKEQDDKDLKENTLDNLEEATENDLSQKVNTLDELKKWVQKESTDVYSQAEVDS
                     LIKKIDDTITTYKNQLEKIKIDNQEATSTETTTESSEVATLQENVSEEPQVVHTNLLN
                     SIEEAAQYLGQKLPQSGGSDAFENGQQNSDGSFTFHYQLHGGPEGITWNIITVNNNGE
                     IIQDDYFKTVPNEATNNNVSTLSQAAISELEAAQLAIAKHNELGRPNATDAGSETTAD
                     GYYFYYMADGTDQMYKYFVSNSGQVQAE"
     gene            complement(1607..1774)
                     /locus_tag="IWI39_002769"
     CDS             complement(1607..1774)
                     /locus_tag="IWI39_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209793.1"
                     /translation="MSIREIVRIQSFPDSYIFVGSSLSAKYKVIGNAVLPKLAKVIGD
                     SIVKQLSESNF"
     gene            1819..2241
                     /locus_tag="IWI39_002770"
                     /pseudo
     CDS             1819..2241
                     /locus_tag="IWI39_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752995.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          982 a          353 c          403 g          725 t
ORIGIN      
        1 aatataacca cttttatgaa aagatgttat aattaactaa aaaataatag ggagaaaaca
       61 catgtactca aagaattgga aaaaaaagtt agtgatgggt gttatggtag ggtctatttt
      121 tgtttttgca ggttgttcag cttcgacaga caaaagtaaa acggctactt cagaaacaaa
      181 aactgctgaa gtagtaaaaa gtaaaatgaa acaaatagaa aaaaatgtag agaaaatttt
      241 agccgaaaaa gataaagata caaaactgtc ggagtacaaa aaatttgtta ctgaatccag
      301 tgcttactct aagtcagata aaacggagaa aaaaattact aatacctata cagaatctat
      361 taagaagatc aaaacaaatt ttaaagaaca agacgataaa gacttaaaag agaatacact
      421 agataatctt gaagaagcaa ctgaaaatga tctttctcaa aaagtaaata cattagatga
      481 actcaaaaaa tgggtacaaa aagagagtac tgatgtatat agccaagcgg aagtagattc
      541 attaatcaaa aaaattgacg atacaattac cacatataaa aaccaactag agaaaataaa
      601 aattgataat caggaggcta catcaacaga gactacaaca gaaagtagtg aggtagctac
      661 tttacaagaa aatgtttccg aagaaccaca agtggtccac acgaatttat taaattctat
      721 tgaagaagca gctcaatatc tgggacaaaa acttccacaa tcgggcggtt cagatgcatt
      781 tgaaaatggg caacaaaatt ctgatggctc gtttactttt cattatcaac tacatggagg
      841 tccagaaggt atcacttgga atatcattac agtgaataat aatggtgaga ttatccaaga
      901 tgattatttc aaaactgtac ccaatgaagc aacaaacaat aatgtctcta cacttagtca
      961 agcagctatt tcagagttag aagctgctca acttgcaata gctaaacaca atgagttagg
     1021 tagacctaat gcaactgatg ccggaagcga aactacagca gacggatatt atttttatta
     1081 tatggccgat ggaactgatc agatgtataa atattttgta agtaattctg gtcaagttca
     1141 agcagaataa cattgagtta taatgatatt atcagatgaa ctgtatcata aatttttaac
     1201 gtgaaatgtt tgtaatatca attggagaat ggtatatgga tttactaaaa gataataact
     1261 gttatttttt agtaaattca tactatttag aaaagaataa tttgctgaat acttgtaaac
     1321 tatgtgtgat acttatctta tatggatata gactcgtggt gctagttaat ttcagcttat
     1381 tataaaccaa cattttcatt ttataaaaac gactaaaaac agaatttctg agtaaaattt
     1441 caaaagtaaa aatagtgata ttatgctctt gttcctagat tcaataatta actagcacca
     1501 cgagtttata aaattggcaa taaaatttta atcattatta acataacctg gatctgtaaa
     1561 gcaatttaac tcttaataaa aaagcatcta aaaaaagcgt gttcagctaa aagtttgatt
     1621 cagaaagttg tttaactata gaatcaccaa ttacttttgc aagttttggt agaacagcat
     1681 taccaattac tttatatttt gctgaaagag agctaccgac aaaaatataa ctatctggaa
     1741 atgactgaat tctcactatc tctcttatcg acactcttag atgaactcaa aaaaatgggt
     1801 acaaaaagag aatactgatg tatatagtca actagaaata tcgctcatag agtgcgtgtt
     1861 cgaggtcgaa aagaaatctg tgaggagttc aaattgattt accatgcgaa ttcaaaagaa
     1921 gaggcattga attacatcga ttttatgatt aggaaatgga aaaagcagga tcgaagagtc
     1981 gtgaacctac tcttgaatcc tgccctatta accttttata atttccctta cgctatcaga
     2041 cgaacaattt attcaacgaa cctgattgaa ggctttaata agcagctaaa acgatatact
     2101 cgaagaaaag aacaattccc taatgaagaa tctctctaga gattcttagc ttcgcaattc
     2161 aactaatata accagaaatt tttaagtaga gttcacaaaa gatttaaaga aattcaagat
     2221 acattagagt cgatgattta actgtaatta acagaatgga ttttccattt acacataatt
     2281 cttgacgcta cctcataata attaagagct ttaaaattag tatttattag atacttaaaa
     2341 gctattttag tattgataaa ttgttaccag caacaaaaaa gaagttactt agtgctaact
     2401 tttgagcaac tgctagagga ggtaggatat ggctaattat cctgtaattt ttattgtatt
     2461 ttc
//
LOCUS       DACDOA010000183         2388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDOA010000183 DACDOA010000000
VERSION     DACDOA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.189"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(733..1911)
                     /locus_tag="IWI39_002771"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="IWI39_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="IWI39_002772"
     CDS             1941..2351
                     /locus_tag="IWI39_002772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209794.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT          768 a          382 c          439 g          799 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttga
//
LOCUS       DACDOA010000184         2375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDOA010000184 DACDOA010000000
VERSION     DACDOA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.190"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            107..>2375
                     /gene="esp"
                     /locus_tag="IWI39_002773"
     CDS             107..>2375
                     /gene="esp"
                     /locus_tag="IWI39_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9209795.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          857 a          360 c          480 g          678 t
ORIGIN      
        1 ctagttaaca taagtttagt aagattccta ttctgtgaat aatacgtatc agatttatct
       61 tttattctta ctaagaaaat aactattaag ggagttgatt tgaataatgg ttagcaagaa
      121 taataagaga gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa
      181 attaagtgtt ggggttgcat ctgttttggt tggtgttggt cttgtcttgg gaacaactga
      241 actagttcaa gcacaagatg aaatttctcc aagcacccca cttgaaacag cgataagctc
      301 cgtacaagta ggtgacaaag tagcttcggg gaatactttt caggagaatc ctggttatac
      361 gaaaaattat aacttttcgg atttacaatt tagtcctcaa gaactaactg gagacacact
      421 aaaaggcaat acaattggat ttgaagttta cggaaaacat aatattgcag catcaactaa
      481 aaactgggaa atccgtcttc aattagatga acgattagct aaatatgtag aaaaaattca
      541 ggtcgatcca aagaaaggaa ttggatcttc tagacggacc tttgtaagaa ttaatgattc
      601 gctgggtaga cctacaaata tttggaaggt caattatatt cgtgcaagtg atggattatt
      661 tgctggggct gaaacaactg atacacaaac tgctcctaat ggggtaatta cgtttgaaaa
      721 gagcttagat gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat
      781 gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa attgtaccag gtattgatag
      841 tacgggctac tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa
      901 taatcctgac cagtttaaac atggatcagt aagtgcaaaa tatgaggaac ctaatactca
      961 aacaaaagat ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt
     1021 aacgaaaaat tataatttct catattcagc ttccgcaaag ggcacgcctt ggtatgctaa
     1081 ttacaaaatt gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca
     1141 ggctgacaaa gtaacatatg atgtttcttt tgaatcaggg aaaaaagtgg ctgatttagc
     1201 aattgaacgg cgcaaagatc atgaaaatta tggtgtaggt tcaattactg ataatgatct
     1261 taccaaactt attgattttg ctaatgcaag tccacgtcca gtcgttattc gatatgtttt
     1321 acaattgact aagccattag atgaaatctt agaagatatg aaagcaacag cacaagttga
     1381 agaaaataaa ccatttggtg aagatttcat ctttgattct tggttgtcgg atacgaataa
     1441 aaaattaatc cagaacactt atggaacagg ttattattat ttgcaagata ttgatggtga
     1501 tggaaaccct gacgataaag aagagagcgg agacacgaat ccatatatcg ggaaacctga
     1561 attagaagaa gtatatgatg ttgacacaac agttaagggg aaagtattca tccacgagtt
     1621 agcgggaaca ggtcacaaag cccaacttgt tgataaagaa ggtactgtat tagcagaaaa
     1681 aactatcgct ccaaatgaaa aagatggggc tccaatttca gatactgtag aatttgaatt
     1741 tacgggtgta gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc
     1801 tccaggtttt gataaaccag aagaaggttc aaccgttatt aaggaatcac caaaagcggt
     1861 tgataaacaa accgtggtag ttggatttaa accagatgct aaagaatcaa ttcggaataa
     1921 taaaaactta cctgaagatg cagagtattc atggaaaaca gagcctgata cttctaacgt
     1981 tactgatagt acgaaaggta ttgtaactgt taagatcgga aatcgaactt tcgacgtgga
     2041 tgtagagttt gctgtaaaag cttctcaagc tatggaaaat gatgcaacat acgtacctat
     2101 aacaacaact ccagaaacga cagttcaaag tggaaaacct acatttgata aaccagatgt
     2161 tcctttagct aaagatgcct tttcaatttt agatgtctat aataaggact tcggtaatgc
     2221 aagtgttgat gcaaatactg gtattgttac attcactcca gctaaaggtg taggagaatc
     2281 ggagccgatt actggaacaa ttcctattaa aattgtttac caagatggtt ctgtaggcac
     2341 gaccgattta gcagtaactg taagtaaaga tattt
//
LOCUS       DACDOA010000185         2365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDOA010000185 DACDOA010000000
VERSION     DACDOA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.191"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..2215
                     /locus_tag="IWI39_002774"
     CDS             62..2215
                     /locus_tag="IWI39_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9209796.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          812 a          416 c          529 g          608 t
ORIGIN      
        1 gtttattaag aagtttttgg gtacaatgaa gcatgaatgt aaataggcgg aaggatagga
       61 aatgatgaaa gattttatga aaaaaatatt aaatacttct tggttagaaa aaataagaaa
      121 aggtgcacaa gaacctaaga acaaaacgtt tcgccggtcc catgttcctt ttcgcttaaa
      181 ttttcttttt ttcattatat ttacgttatt tgttgcgttg attgctagat tgggttattt
      241 gcagatcgtg aacggcgaag agatggaggc gcgagtgaag tctacttcta cgattacggt
      301 ccaagaaagt actcctcgcg gaatgatcta cgactccaca ggaaaagctt tagttacgaa
      361 taaagcgaat caggccatca catatacgag aggaacacaa gttacggcta aagatttgct
      421 tgaaatcgca acgaagctga actcgctgat cgatgttccg gtagatgaaa atctgacaga
      481 tagagataaa aaagattttt ggctggctga tcccaaacat ttgaaagaag ccacaaaaag
      541 actttcggca aaagaaaagc aattagggaa cagcgaacaa tatagtgcga tggtcaataa
      601 agtcacagct gatgagatta atttaaacga ggagcaatta aaaattgcta ccatctttaa
      661 acggatgaat agtgcatatg aattaaacac tgtctatatc aaaaactctg gtgttactga
      721 tcaagagtta gctgttgtgg cagaacgcgc ttctgaactt ccaggagttt cgactggcac
      781 agactggaca cgagaatata atgcagcaag ttcactgaaa agtattcttg gcagtgtgac
      841 gactgaaaag caaggacttc cagcagatga agcagagaaa tacctagcaa aaggttattc
      901 tcggaatgac cgtgtaggac aaagttattt ggaaaaacaa tatgaagatg tcctgcaagg
      961 aaccaaaacc caatatgaag tttcccttga taatgaagga aatgtttcga atcaaaaaga
     1021 aatcttctct ggggaaaaag gttctaattt aatgttatct atgaatgcag aattccaatc
     1081 aaaagtagaa gaaattttga aaaggaatta tcaaacattg atcaacaacg gcaaagcaca
     1141 gtattcgcct ggtgcttatg cagtggctat gaatcctcag acaggagaag tgctagcaat
     1201 gactggtttc agtcatgaac aaggttccaa agaaatcacc gagaatgctt taggcacgat
     1261 tactagcgcc tttgatccag ggtctgtagt caaggctggt actttgacag caggctgggc
     1321 aagtaacgcg atcagcggaa atcaagtttt gattgatgag ccgattcgtc tgcaaggtgc
     1381 ttcagaaaaa agctctgtct ttaatcgaag cggacaagtt gctcttgatg cagtcaaagc
     1441 atcggaattg tcttctaata cgtatatgat caaagtggcg ttaaagatgt taggactaga
     1501 ctatacacct ggaatgggac tacctagttt agatgaagaa gctaaagctt atcagcaatt
     1561 gagagactct ttcaaagagt ttggtcttgg tacaacgact gggatcgatt tgccgaatga
     1621 atctcctggg atctcacgtt ctgttgatta tatgaagaag ttcaatgcgg ataatggcaa
     1681 agaatggtat gctccaggta actttaccga tttggcattt ggacagtttg acacatatac
     1741 accaatccaa ttagctcaat atgcttcaac agtcgctaat ggcgggaaac gagtacagcc
     1801 gcacctagtc aaagcgatct atggcaatga cgaaaatgga aacttgggtg aagtgaagaa
     1861 agaaattggc acaacagtag aaaatacggt caatatttct gcagaaaata tggcgatcct
     1921 ccgtgaagga ttccaccaag ttgtccatgg taccgatcct tacacaacag ccaaaccatt
     1981 agccagtgcg aaaatggacc tttccgcaaa aacagggact gcagaaacgg ttgcggaagg
     2041 tcatccagat attgcgacag tcaatagtaa tatcgtggca tacggaccaa cagacaatcc
     2101 ggaaattgcc atcagtgttg tcttgccgaa cttattagac gaacaagacc acataaactt
     2161 gacgattgct aaagaaataa tggacgcgta ttatgatatg ttcatggcaa aataaaaaag
     2221 aggtcgtgaa aaagccgctt agtttcgagt atgaagaaaa attttgtgaa aatagcttct
     2281 cacatttttg cttaaatttg acttcatacc gagaaactgg cttttgaaca ccgtttactc
     2341 ggcaaaaaag gttgtgacaa aaatt
//
LOCUS       DACDOA010000186         2364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDOA010000186 DACDOA010000000
VERSION     DACDOA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.192"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(526..843)
                     /locus_tag="IWI39_002775"
     CDS             complement(526..843)
                     /locus_tag="IWI39_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9209797.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(844..1098)
                     /locus_tag="IWI39_002776"
     CDS             complement(844..1098)
                     /locus_tag="IWI39_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9209798.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1358..1576)
                     /locus_tag="IWI39_002777"
                     /pseudo
     CDS             complement(1358..1576)
                     /locus_tag="IWI39_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          769 a          431 c          347 g          817 t
ORIGIN      
        1 atagttattg tacctatatt ttatcccatt ccttttaaaa caagaatacg tacaaagtaa
       61 aatgttgtaa atagtctagc agggatgtat attttcatgt accaacataa tatttaagaa
      121 gtttcccata gatatattgg acttcctctt ttaaaacaca aaaataaata ctaccttatt
      181 taggtagtat ttattaaaag ttacgtcaat tttgattaaa aatatactac ttgaacctct
      241 catgagtaaa gtcacgaaat ttttgggaat agagcgtact tgatatctta ttttttcact
      301 aatagcagtg tctcttattc accaagcgat ccccgtagtt cctacggttc ttaatttttc
      361 tctagactaa tgattgtatc tttagacctt cggccataac atggagcaaa ataaaaaggt
      421 actaagtctt atatatcaat cttcgagtaa ccgacggtta acaaccatat cgagcggcat
      481 taatcaccca cctgtagagg atgagcgact tttgcctaat tattgttaaa agcctagttt
      541 catttgttta gattggaaaa tacgtcctac aagtatctct tttttttcat ggtcgatccg
      601 ataaaataag gcatagcttt tgccgatcac gatccgatat gttgggatat ccattccata
      661 gatttcggaa acttcctcat gcatagttgg aaaatttttg gttagttcta aagccttctg
      721 gatcgcacct acaaatcttt ttatgctggc tggagaaaga gcaagctcat tttcccattc
      781 ttctattatc gtttcaaggc tggtctggaa acttttagaa taaactagcg tgtactcttt
      841 catctattca ctccagccgt attttccgaa agcttttacc atttcaattt ctgaaactgt
      901 taaacctttc tcaaagtctt ggaaactttc ttcaatcaat ttgcgatcgt gttcttttgc
      961 tatttgagta gtatctaata tgaataagtt gccttcttgt ttggcttgga tagtgtctcc
     1021 gctttttaat ccaagagagc ttggaaggat cactcccacg ctattgccga tctttctaac
     1081 ctttagttct tttcccatgt tatcacactt cttttagttt ttgataaata aattataaca
     1141 tgttataacg acaattgcca acggtgtttc ttaagggatt ccactccata ttattattgg
     1201 acaaaataag ccacttagaa cgctgaaaac atatacaaac gatatcaaaa tacgagtgaa
     1261 ctactcacca tacacttccg ttaacatcca tttaagtagg tatttttatt atatatagat
     1321 ttaacatatg tcggtcggtt gattgatata gctaaagtta taactcaacc gaagccgatt
     1381 catctacacg aataaagttg cgagtgttct cggctaattc ataaaaattg ttatgtgttc
     1441 catatagcaa tactggaata actttatttt cttgaatctc ataagcaact ctatacacgg
     1501 tgaccgcata cttaaatcca ttagaccaat tacctgataa acctccagtt ttttgctctt
     1561 acttaaatgg atcaatagca atttcatcac aaacctatag aggatgagtg acttctgcct
     1621 aattacgtta aaaaatattg aaagtaactc tatgtagcaa aaaatatcta gaatttcatt
     1681 tcctcaaaac tgatcgatat tgtatctaaa aatatatact acctaaaata ggtagtatat
     1741 attaaaatat gtgcttttgt tagaaaaatc aatacataaa ttatcatatc tgtatatgtc
     1801 atttaggtat ttcagttatg ttataatagt tttgctgtct gctaccccgg cctaatctgg
     1861 caaggggagg aggagatata atgaaatacc tttttgattt tgtttttgca atcaagccat
     1921 ttgcagaggt aactgcacac tacatctgca agtggcttga ttgcaaaatt gccaaaaaag
     1981 acagtaagcc taccagttaa ttctataata tagaaacgta aaaaaaaccc catcagccta
     2041 cacgctgatg gggtttgatg atataatgaa atacctcttt gttactcata attataccat
     2101 aattagtcac gaatgcaacc aatagaaaaa actacttaga acggtagatc atcgctaata
     2161 acaatgcatt gtccaccaaa tggattttgt gaagcattaa atacattgtt attaattgtc
     2221 gctgttttta actaggcctc tccccttcta ttataaaaga agatatatat tatttcttaa
     2281 ttacctacta ccttaaacca aaatttaagt actacctaat ttaagtagta cttaaataat
     2341 ctaaaaaata gttatcccaa ttgt
//
LOCUS       DACDOA010000187         2318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDOA010000187 DACDOA010000000
VERSION     DACDOA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.193"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..188
                     /locus_tag="IWI39_002778"
     CDS             <1..188
                     /locus_tag="IWI39_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9209799.1"
                     /translation="RELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFPFLYPGKNEIKW
                     TKNIHSFEIMPRWWRKV"
     gene            185..>2318
                     /locus_tag="IWI39_002779"
     CDS             185..>2318
                     /locus_tag="IWI39_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209800.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT          849 a          290 c          468 g          711 t
ORIGIN      
        1 acagagaatt atttatagat tctaaattag aagaggctta tcgaaaatta gatggtaatt
       61 tagaacatca ggatcaggtc accttatttt tagattttcc atttttatat cctggaaaaa
      121 atgaaattaa atggacgaaa aatattcatt catttgaaat aatgcctagg tggtggagaa
      181 aagtatgaaa ccaagaatat atagtcctac tgaaacagat ttctcaacga atggcttagg
      241 aattttaaag gattgtacaa gatgtggaat atatgaagta gctaatggaa aatatgaatt
      301 agagttggat tatcctttag gaactagatt tgatgaatat tttgaaaatg actatcaaat
      361 aaaagcaaag ccaaacgatc aagaagagta tcatatcttt tttattgatg ataaagatat
      421 agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc gacttggaag
      481 acgggtagtc actcttgtgg aagtggactc taaaactggt caagaagcga tatcaattat
      541 tgaaactaaa atggataaaa aatctgacat acgactttat tctgatatta cggctgtttc
      601 tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac aaggatcatt
      661 gcttcaatat tggggcggag aaattaaacg tgaaccattt aagctttctt tgttaaagcg
      721 aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg gtttgaaggt
      781 caaattagat tggacaggta taaaaacaag aattattcca tatgctgatc ctcaaagtga
      841 agtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata ttaataacta
      901 tcctgatgtc tatacagaac atgttcagtt tacagaagaa caaggagtaa aagacattaa
      961 gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct gtgataagcc
     1021 taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag cgaaagaatt
     1081 tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata aaaaatataa
     1141 tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg aaaaaacttt
     1201 gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg taactattca
     1261 ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg attatgtttc
     1321 ttcaatgatt acaggtcaag gcagtgcagg aggatatgta gttttatggc caaaagaaaa
     1381 accttccaac atctttatta tggataatcc agatttagaa aaggcaagag aagtactaag
     1441 aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt ttaattcagc
     1501 ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa tcaaagcaga
     1561 tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta atggactact
     1621 ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa tggaattttg
     1681 gaattctaag ggttcaattg gaacgattgg aacaactgat tctgttggta atccttttcc
     1741 tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta caaatggaga
     1801 cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat taggaaatgg
     1861 taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta aaggaaaaga
     1921 aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg gtgggtatcc
     1981 tagtgaatta aaaacagatg cagaaaagag agcttggaga atttacgata ttttgtgtaa
     2041 taacggattt acgaaacaat ctgcatgtgg aatattgggg aacattcaac aagaaacagg
     2101 aggaactttt gatcctgata ctgttcaaat aggtggacca gcatacggat tagttcagtg
     2161 ggatggttct tcatatcctc ttgttggtcc agcaacatgg gatggaaaag tttatgttca
     2221 aaacttattt aatgctgcag gtattaaaga accaataacg agcttagatg cacaagttcg
     2281 attgcttatt tggacattta caaatggaca atggatgg
//
LOCUS       DACDOA010000188         2303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDOA010000188 DACDOA010000000
VERSION     DACDOA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.194"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(196..780)
                     /locus_tag="IWI39_002780"
     CDS             complement(196..780)
                     /locus_tag="IWI39_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9209801.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1115..1345)
                     /locus_tag="IWI39_002781"
     CDS             complement(1115..1345)
                     /locus_tag="IWI39_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9209802.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(1360..2286)
                     /locus_tag="IWI39_002782"
     CDS             complement(1360..2286)
                     /locus_tag="IWI39_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9209803.1"
                     /translation="MGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVTNSILADI
                     TENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGGNAWFTKL
                     VKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNIEKQLAEK
                     DPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPS
                     AYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTI
                     FTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT          727 a          417 c          295 g          864 t
ORIGIN      
        1 aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt
       61 gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca
      121 ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg
      181 caagcttttt tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa
      241 tcttctggtt aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa
      301 tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg
      361 attgatcatt ccgatagtct ttaacttatc tagacaacag actgtctttt ttgtcacaaa
      421 tgatgtaatt ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaaccaa
      481 atcgaatata cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt
      541 ctctctccaa cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc
      601 ttcattatac atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc
      661 ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaagcacag
      721 ttgccttatc ttagtttgct gaaatcctac tttttcaaaa tggcttagtc cactttctat
      781 taatttttgg tgagttgtaa tataatctct ggtttctatg ggtacttcct cctaataaga
      841 tgaattaact atattatctt acacaggact tgaagtttca atcgttaacg tctattactg
      901 aactcattcc ttagagctaa actaatgaga aagagcaaaa taactgatac aaaaagcaat
      961 cctacaagaa ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag
     1021 atttaaaaaa attatatcca gctattagga taagtcctat cataatcact ccaatagcca
     1081 ttttcttatc ctccctaaaa ataaagcctt ttacttaaaa cctttttttg taatcgtcaa
     1141 taaatttttt ccctttatca gttaattgaa atcctatatc taaatagtcc tcatatctaa
     1201 gtttagtcag catctcactt acagatgctg gactaacact tagaactttt gctaaatcat
     1261 tattttttaa tttctttttt ctttttgata attcctgaat tgctagtaaa taaattagta
     1321 tagtagttgt tttaatttgt accataccta ccctccaaat tatttcgaaa ggccttcagc
     1381 aattttatcc aggttccatt tcatcatcgc atagtaacta tcaccatctt gtcctttttc
     1441 tgcaattgaa tcagtaaaaa tcgttgaata gataggaata ttggtatctt ttgatactgt
     1501 tttcattggt ctatcgtcca cactgctttc tacgaataag gagggaactt ttgttgtgcg
     1561 taatttttca actaagtgtt ttatttgatc tggtgttcct tcttcttcag tgttgatttc
     1621 ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa tatttaaagc atccttcact
     1681 tgtaacaatc atttttttat cattcggaat tgaagcaaat ttagatttag cttctttgtc
     1741 tagagaatcc aatttttcaa tatacttatc tagattttct ttatagaatt ttttattatc
     1801 aggatctttt tccgctaact gtttttcaat atttttagcg taaataatac cgttttctaa
     1861 atttaaccaa gcatggggat cttccttccc tttctcactc tgaccctcta agtaaataac
     1921 atctatgcca tcacttgctg caaaatagtc tttgttttcc tctttgttcg cattttttac
     1981 taattttgta aaccaagcat ttcctccagt ttccaagtta acaccgttat aaaaaatcaa
     2041 atctgctttt gaagtttttt gaacatcttc aggcaaaggt tcatattcgt ggggatcttt
     2101 cccaataggt acgatactgt gtaaatcaat tttatctttt gctatatttt cagtaatatc
     2161 tgctaaaatc gaattagtaa ctactacctt taatttttcg ttcgaagcca ctgtatcttt
     2221 gtctttacta tttgttgaac atgccccgaa tataaaaaga gcaatcaaaa tccccagact
     2281 tcccagtact gctttccatt ttc
//
LOCUS       DACDOA010000189         2295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDOA010000189 DACDOA010000000
VERSION     DACDOA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.195"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            130..1743
                     /locus_tag="IWI39_002783"
     CDS             130..1743
                     /locus_tag="IWI39_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9209804.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          717 a          408 c          447 g          723 t
ORIGIN      
        1 taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt
       61 ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga
      121 gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact
      181 cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt
      241 tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg
      301 ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa
      361 cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca
      421 aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat
      481 tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg
      541 gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt
      601 ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg
      661 actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta
      721 gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag
      781 atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg
      841 ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa
      901 ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc
      961 tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt
     1021 atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg
     1081 cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca
     1141 agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact
     1201 gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta
     1261 aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa
     1321 ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt
     1381 gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg
     1441 aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca
     1501 agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat
     1561 aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat
     1621 cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat
     1681 acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa
     1741 taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa
     1801 aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa
     1861 gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg
     1921 cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca
     1981 aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat
     2041 taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat
     2101 aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta
     2161 ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg
     2221 tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt
     2281 tcctatttat ttaac
//
LOCUS       DACDOA010000190         2252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDOA010000190 DACDOA010000000
VERSION     DACDOA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.196"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..1921
                     /locus_tag="IWI39_002784"
     CDS             59..1921
                     /locus_tag="IWI39_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209805.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1921..>2252
                     /locus_tag="IWI39_002785"
     CDS             1921..>2252
                     /locus_tag="IWI39_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9209806.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          807 a          336 c          445 g          664 t
ORIGIN      
        1 tttcatattg tcgttagttt agaaaacgct tatattagca atgtaaggag agatgataat
       61 gcagcagcta acaaaaagag aaaggcagct tttgatttac ttggtggaaa tgtctgaacc
      121 ggtaacaatc aaaaagttgg ctgaaaaaga aaatgttagt gttcggacga taaaatacga
      181 tctagatgat ataagagagt ggctgaatga gcggaagcaa gtcctatata gcaaacgtag
      241 tcagggaatt tggatgaata ttagtagctc tgatcgaatc aaattaaaaa gcgaattgat
      301 ggatgttaat cgttttgaaa tatttgcaga tcaaagtctc cgaatcaaac gtttactaat
      361 tatgctttta gtaacaaaag aagctattac tggaatgaaa atggccaacg cattagatgt
      421 cagcaaagat acgatcatga atgatttaga tgtgctggaa aataattttc ttaaggaagg
      481 attgaccttg aatcgtcagg cgcgaaaggg tttttggatc ataggagaag aacgtctagt
      541 tcgattaacg attgaagaga ttcttcaaaa agagttcacg gattatgaca tttataaatt
      601 aatgtcattg ttattgaatg gcggtgagac tgaatacttt gaaatgtatt ctgctactgc
      661 tacacctatc caagaggtat ttaatcaagt aatcattcga atgcgacact tactggaatt
      721 tgaaaatcta gagaaactga attacgcgga attactaaat attctgattc gagtgacaat
      781 tgctaccgtt cgcttacgca aagaggctac gattggtcgt tatcaattgg ttgctgaaca
      841 agaattagac aaaaacgatc tttcttatcg cgtcatgcag caggtctttg attattatca
      901 gttgccactg ttcgaggatg aatatcatta tatctatagt gatacattcg acacgattca
      961 ccagcaagac gtcatgaaac tagttgagaa tgtgatttat aaagtaagta aagcaatcaa
     1021 ccattctttt tatcaagatc cacaattgtt gacgaatctg tatgctcatt tatccatgcg
     1081 attatcgaga aagaaaaaat ttgtcaatga atataatcct tttaaagaag atatcaagca
     1141 gaaatacccg gaattatttg atgcgatcaa gcaagcagtt cgacaagaaa taagtgaaca
     1201 aaatattctg atcaatgatt ctttcgtggc atatattgcc ttacactttt tggtttccta
     1261 cgaaagagaa gaagatctac gaagcgttag agccgtctac atttgttcaa cagggttggg
     1321 tgtgactagt ctgatcaagc aaaagatcaa tgaagaatta aataatatca ctatcgtggc
     1381 atttgcttca gtattgaatg cgcaagaaat gattgctacc aaagatcctg atttggtgat
     1441 tagtattttt ccaattaatg gcatcgatcg tcctttcatc aaagtccatc cattacccac
     1501 tgaacaggac ttgaaaaaaa ttcaacaaat aataaaaaaa atacttgctt cttccaatca
     1561 gcaggaaagg cataacttga atcttagaga aacgtccatc aataaagata ctttagaaga
     1621 ctttagcaag gaattgattt tgaaagctta tacgatttat gaacgtttac ttgtcatttt
     1681 ggaagcagaa ttgatccaag aatatcgaga agcttttttg cttcatgtat tcttaatggt
     1741 tcatcgtatc acatttgatc aacaatatat ggcagaaggt cgtcaagtag accagattat
     1801 tttagacaaa ggaacactgg taggaaaaat cgaagcagtt tttgcagaaa ataatttagg
     1861 aatcaatcag tcagaaatca gtgcattatt ctcatatata aaagaaggag cgtcaagtta
     1921 gtgaaaatgt cagacgaggc tttaaaaatt atcgaggaaa gtcaaaatcg ggaaaagtta
     1981 aaacaagtga ttgatctaat tgaagagcga ttagtagaac atgctattgt accaaccgag
     2041 ttgcagtgga ccattctaat taatcacttg aatgagatgc tgaaacgttc tcaaagaaag
     2101 gaaacgattc ccgtagtcga tcgggatttg ttcaaagaag tatccaaaga agccataact
     2161 atttctgatg aaatagttag aagtatcggt aatttaaccg atgatgaaat atatgttttg
     2221 tcgattcatt ttgaaacagc aaaaaataat ta
//
LOCUS       DACDOA010000191         2234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDOA010000191 DACDOA010000000
VERSION     DACDOA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.197"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1241
                     /locus_tag="IWI39_002786"
     CDS             <1..1241
                     /locus_tag="IWI39_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP9209807.1"
                     /translation="VALDKENELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPP
                     KNNKAWSKKDILQIVKKNVTGANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGF
                     LFTKQSGSIKDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAE
                     NLGPLGVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPY
                     LCVEVESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEP
                     YGIGGHTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFI
                     PYNAIASTECAINAIKLFHKVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGV
                     NKFEERFFSKYCKVCGELDD"
     gene            1234..1695
                     /locus_tag="IWI39_002787"
     CDS             1234..1695
                     /locus_tag="IWI39_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209808.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1708..>2234
                     /locus_tag="IWI39_002788"
     CDS             1708..>2234
                     /locus_tag="IWI39_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209809.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVN"
BASE COUNT          797 a          281 c          436 g          720 t
ORIGIN      
        1 tggttgcttt ggataaagag aacgaattac aattaaatgc agcgagtaga ttgtttaatg
       61 gagagaaaga agatactcat gaattagatg catactatgt tccaattaga aacaagaata
      121 atattatgcc cccgaagaat aataaagcat ggtcaaagaa ggatatttta caaatagtaa
      181 aaaaaaatgt aacaggtgca aataaaagat tttttttaaa acttgaaaaa agaaagaata
      241 gacataatgt tttacttatt ctagatatcc caattgaaga aaagtcaaat aatgtcataa
      301 ttggatttct ttttacaaaa caatctggat ctataaaaga ttataagggt acgcctagga
      361 ctgtattagt tcagcgtcat gatgaattat atttggtgaa tagaacgagc ggagaaaact
      421 atggattggg tgctaatata gctattgttg gggtagggtc agttggcagt aaaattgctg
      481 aaaatcttgg tccattaggt gtgagaaaga tgacactcat agacaatgat tgttttcaag
      541 ctgaaaattt gtatcgaaat tcacttggtg cagatagttt aaaaaatgcc cgatctcaga
      601 ataatttttt taaagttgat ttaatgcagg aggagctcga aaaaaaaatc ccataccttt
      661 gtgttgaagt ggagtcaatg aacgttttag acttaattag tatgcaacct ggttttttca
      721 aggaatatga ctatgttttt gtttgcatag gagatacaat ggcaagcctg gctttaaata
      781 attatttttc caaaagtagt caaaaagttt tctattcttg gctggagcca tatggcattg
      841 ggggacacac tctatttata aattattctc taaatcaaaa aggttgtttc aattgtcttt
      901 atacagattc aaactctaaa aataggtgtt caaatagagc ttctctggta aaagaaggac
      961 aaatttttga aaggacttta gcgagttgcc gctcaaaatt tataccatat aatgctattg
     1021 cctcgacgga atgtgcaatt aatgcaataa aacttttcca taaagtcaat caaaataata
     1081 tgaccaatag tactcttttt tcatggatag gagatgggag tacttttgag aatttaggat
     1141 atcaatatag cgacagaaat acttttctta atggagtcaa taagtttgaa gaaagattct
     1201 tttctaaata ttgcaaagta tgtggggagt tagatgatta aattttcgtg tgaccgtatt
     1261 gtcattgaag taagggatga agttcttaag atatttgaac aatatcggca gggaaaggat
     1321 gacttgcctg aagctggagg agttcttcat ggaaaaagac ttaagaatgg gagcataata
     1381 attaccgata taacaacacc tcaaatagga gatatacaaa aaagattact ctttaaaaaa
     1441 aaccgagaaa ttcatcaaca gctttctaat gagaaatgga tagaatcaaa taagttttct
     1501 attgcattgg gagaatggca tactcatcct gaagctgtac caactccatc aaatgttgat
     1561 aagaaaagct ggagactgaa cgtgagcaag caacatgatg atagagttta tgtttttatt
     1621 atcgttggat tttctgattt gagaatgtgg ttactgtcaa aaaaatcaga tattaggcag
     1681 gctataaaac tataaaggag tgattaggtg aaatcacaaa agaatatttg gtggtggggg
     1741 tttttagtag gaaaggtaat gataccagcc cttctcttaa tttctctacc gatgattact
     1801 tttttcttta ccaataaaaa aatgagtaaa gaagccgtga tttttttctt tggagaccaa
     1861 aaagctacct ttattgaaac gttagtaaca tctcttcagc ttaatttgaa ttacattttt
     1921 tcaattatta ttgttatatc gttattgaat ttttttaaaa aaagaaattc aggaaaggtg
     1981 tttaactcta atggaaatgt gtactacaac tatttttatt ttgtattttg ggtagcagca
     2041 actttacttg gttatgataa aattcagatt gcaggaattc ctatacacat gcaatataag
     2101 ttggttttga gtggaatttt ttctgaggtg ttgccagata tatacgatga tcattacgac
     2161 agtgatggaa catgtaaagt aagcattgaa aaagaaaatt tcgatgacat cgatggatat
     2221 gactctgtga actt
//
LOCUS       DACDOA010000192         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDOA010000192 DACDOA010000000
VERSION     DACDOA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.198"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWI39_002789"
     CDS             <1..378
                     /locus_tag="IWI39_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9209810.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWI39_002790"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWI39_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9209811.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IWI39_002791"
     CDS             1859..2026
                     /locus_tag="IWI39_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209812.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IWI39_002792"
     CDS             2080..>2232
                     /locus_tag="IWI39_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9209813.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDOA010000193         2207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDOA010000193 DACDOA010000000
VERSION     DACDOA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.199"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            125..901
                     /locus_tag="IWI39_002793"
     CDS             125..901
                     /locus_tag="IWI39_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9209814.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            916..1119
                     /locus_tag="IWI39_002794"
     CDS             916..1119
                     /locus_tag="IWI39_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209815.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1135..1473
                     /locus_tag="IWI39_002795"
     CDS             1135..1473
                     /locus_tag="IWI39_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9209816.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1460..1639
                     /locus_tag="IWI39_002796"
     CDS             1460..1639
                     /locus_tag="IWI39_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209817.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1682..2152)
                     /locus_tag="IWI39_002797"
     CDS             complement(1682..2152)
                     /locus_tag="IWI39_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209818.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          816 a          319 c          418 g          654 t
ORIGIN      
        1 atcaatgttt ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg
       61 tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag
      121 aaatatgaac acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt
      181 aaatgatgaa ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac
      241 taaagatgct ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac
      301 ctcaggtcaa tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt
      361 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc
      421 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
      481 tccagataca atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat
      541 cgcagaacaa aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc
      601 ttctacagat tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca
      661 gatgaataaa ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt
      721 gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc
      781 cgatggtggc actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat
      841 ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta
      901 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
      961 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
     1021 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
     1081 agaattatag attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa
     1141 tatctagaag caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat
     1201 gaggagttaa aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa
     1261 cggattaaca gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga
     1321 aatattatcg atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga
     1381 gacacatatc tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa
     1441 atcttaagga ggcaagcgga tgggaaaatt taatagagcg ctagtgttca gcgcaccgct
     1501 aatcatctac gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta
     1561 catggtttgg atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc
     1621 aaccgagggg gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta
     1681 ctcagtttaa ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa
     1741 ttttgtggat ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt
     1801 tattaaaatt aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag
     1861 gaatatttag cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag
     1921 caattaattt cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt
     1981 ctaaattaac taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa
     2041 tcatatctgc agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt
     2101 ttatttttat ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa
     2161 aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag tttttaa
//
LOCUS       DACDOA010000194         2153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDOA010000194 DACDOA010000000
VERSION     DACDOA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.200"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..591
                     /locus_tag="IWI39_002798"
     CDS             <1..591
                     /locus_tag="IWI39_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9209819.1"
                     /translation="GLIMLIVRFIKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDN
                     NKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTK
                     YISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTF
                     TKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            704..1339
                     /locus_tag="IWI39_002799"
     CDS             704..1339
                     /locus_tag="IWI39_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209820.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1638..>2153
                     /locus_tag="IWI39_002800"
     CDS             1638..>2153
                     /locus_tag="IWI39_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209821.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDY"
BASE COUNT          858 a          329 c          366 g          600 t
ORIGIN      
        1 ggtttaatca tgttaattgt aagatttatc aaaaaaacaa ataagaaaac acctatgatt
       61 ctccttatta tcgggattat ttttactacc gtaggtttct cgctttcctc atcaaacgag
      121 gcaaatgaca ataacaaaca ggaaagctca tcatctgata ctcaaactac tacatctaac
      181 agcagcaata aaagcctaac tcaatttgaa gaatttttag agacaaataa tcatgactgg
      241 ggtgctttct tagatagtta ttattctatt actccagcta ctgagcaaaa tacagctttt
      301 actaaatata ttagtggtaa aacatatact tttgaaggaa ctgttatcga atcaatgact
      361 actagaatag ccattatagc agacaaagag tacgatgata aaagttggaa tgatatttct
      421 gcaacaccta aagtttcata tgtaattttt gcgaaggact taaacaatgc tgataccttt
      481 acaaagggcg ataaagtaac ttttacagga gaaattagtt caagaggatc gaacatagaa
      541 aaatcctatg ctcaatggga tatgattaat agtaaagtaa gtaaaaaata aatgaaccac
      601 gccccactaa gagttagatt gtgcacaata ataaaaccta tacaaaagag tgccatcagg
      661 agtcacaatg cttgtaaagg aaatgacaaa ataggaggta actatggata atagcaatga
      721 aaaactaacc aaaaaagaat atcttgaatt aatagttgaa ggcggtattg gagcaattcc
      781 ttatattggt ggcgttgtgc agtcacttta ttttgggtct aaaaacgaga agcgtttcaa
      841 acgcatcgag aaattctaca aaaatttaaa cgaatcatta gaaaacattc gcgatcaaat
      901 tccagaagat gttttcaact tagagaacaa ggaccaactc attggaatat tcgaagggat
      961 aaatgacgag gtagaaaaat caaaggcaca aaataaaata gatatgtata aaaatcttta
     1021 taagaattgt ttacttcgaa ttaacaacgc ttcatgggat aacgaggagt attatcttct
     1081 tgtactgaat caattaacat cgattgagat tcaattgtta gcatgcttaa tgagtcgtgg
     1141 aaacgaaaat ttcactggaa atatcagcat gtctggctat tcacaagagc taatagatgg
     1201 tagcttaaat agattatctg attttggtct gttagaaaaa acgattagct ccatagtatt
     1261 aggtgatgtc ggcaaacaaa atatgggcta caaaatatct catttaggaa ttcaatttat
     1321 taactcaact cttacttaag ttttctttct aatatgacat ctagacctat cagcaatgca
     1381 acaataaaaa accagacata ccaataatca acaaataatc taaactctgg aagttcagtt
     1441 attcttttaa aaatcacttt aatcataata aacacctctg attggatgat ctaatacaca
     1501 tccaattata ccattaaaac gacaaatagc acatccttgc gccggcaagc aaaaaggatg
     1561 tgcaaaaaat aataaaaccc aaacaatggg cttctttata gttcctattg tatcagagaa
     1621 agagagctga ttcaattatg gcaagattag tcaaacgtgg aaatagttgg caatacgaaa
     1681 tttcatacaa aaaagatgac ggaaaataca cgaagataag aaaatcagga tttaagacaa
     1741 aaggagaagc aaaagatgcc gccaacgaat tagaatataa cttgaacaaa ggccttaaag
     1801 gggatcgcaa aaatctatta ttatcagatt actttgagga ttggatgcaa ctttataaag
     1861 aaggaacagt atctcctatc acttatagaa aatacgaaga tacgttaatg aacataaaga
     1921 aatatatgcc agcggtattg atttctgatt tagatagagt gggatatcaa cgctttttaa
     1981 ataagtatgc taaagaccat gtaaaatcta ccgttattaa gtttaataac catattagag
     2041 cgtcgttgaa agatgccgta gaagaaggat taattccgtt tgatccaact agaaaagcag
     2101 taatcaaagg aaaagattca ttgaagccaa aagaagataa atatttagat tat
//
LOCUS       DACDOA010000195         2100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDOA010000195 DACDOA010000000
VERSION     DACDOA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.201"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            53..703
                     /locus_tag="IWI39_002801"
     CDS             53..703
                     /locus_tag="IWI39_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209822.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            758..1924
                     /locus_tag="IWI39_002802"
     CDS             758..1924
                     /locus_tag="IWI39_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209823.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          797 a          342 c          338 g          623 t
ORIGIN      
        1 tcactttgtg gtttttatct taataagcac attttaaacc acaaaacgag taatatcaat
       61 taaaatatta ctcaatttgt ggttttcatg ctataatagt ttggaattta ctataggggg
      121 cgttactttg aatagaataa aattttatat tgattccagt aaaaagacca ttaaacaaat
      181 tagtacagaa actggtatta actatacaac tcttacgaac tattaccgtg gtgagcgaaa
      241 tcctagaaat actgaaacat ggaatatcct tgctgattat ttccatgttc ctgtaagtta
      301 tttgatggga ttagacgaaa caaattccat agaacaacct aatcttgata gaataaaaac
      361 tgctcgttta gaaaaaaagg acaaaatagt caagaatatt gccagtcttg actcagatta
      421 ttttactgaa ttatctaatc tatacttttc catgacttct ttacttgaag tcaaacttca
      481 agaagatgac tccgactttc tttttttaaa aagtattcta gaggactcct ttgaatacac
      541 gattctattt gccaaacaaa agaagtttat taataaagct ttaaacttaa atatcgatga
      601 aagaaaagag ttagaatctg agttatttgg caacttaacc caagtacagg ttaacaatct
      661 aaaaagagag ataacagctc gattaatatc aaaacttcaa taaaactaat tattcaatca
      721 gccaactcgc caagttatca gattggagag attagttatg tcaatacaaa agtacacaac
      781 aaaaagcggt gcacgatata gtgtccgctt gtatgttcgt aaagatgaaa atggaaagca
      841 agaatactat gtaaaaaaag ggtttaaaac tgaaaaagaa gccaagttac acgaagcaag
      901 aaaaaaggtc gagattgaag ataccggcta tcataagcct tctaatgact tgtttataac
      961 agtgtatgaa gaatggttgc cttcttatag gaatcgtgta caagaaacga catatcaacg
     1021 tacaaaagac cttttccgct tgcatatact accaaaattc gggaataagt ccatttctaa
     1081 gattactcct gttcactgtc agaatgcagt caatgaatgg gctttaaaat tcaagaatat
     1141 caaacagctt aagtcttaca gctctcaaat atttgagtat gccatttttg cagagctatt
     1201 acaccgaaat cctatggcaa atacaaatct accaaagcgg gaaaagaaag aaaatgaaaa
     1261 ttacttttca ttagaagaac taaaagcttt ttgggatatt ctcaataaag aagagccttt
     1321 aaaacatatt ttaatcttcc agcttttgat tactactggt atacgtaaag gagagctagc
     1381 tgctcttcat tggtctgaca tttattttga agaacagcta atgtatgtta gaaagagcta
     1441 tgcaactatt cgaaatgaag agaagaatgc aagaagaaaa actgtcagga ttcaaaaaaa
     1501 tacaaaaaat atatcttccg aacgaatatt accaattgat gcacaaacta ttgaaatgtt
     1561 aaaaatctgg aaaaaagaac aagctcgaga gcttttgcag tttggcataa atacaaatac
     1621 aaaaaatcaa cttatcttta cctatgtaaa tgcagacggt gacattaatc aacctttgca
     1681 tgcggactat tcaaataata tattgaaacg tttagagaag aaatataaat ttaagcacgt
     1741 tactattcat ggattccggc acactcacgc tactcttcta ctagaaggtg gagcaagtat
     1801 caaagaaaca caagatcgat tgggtcataa aaatgctgaa actaccctta acacttattc
     1861 tcatgttact gaaaaagctc aacgaaatgc gttagaaaaa ttcattgctt acactggttt
     1921 ataatattgt cattaccttt ctcattacca gaacgaaaaa aagacacctt ctcgcaatga
     1981 gaaagtgtct gaaatcgttg gtataacaac gattaacgtt ttgaaaactg tgaagcttta
     2041 cgtgctttct taagacctgg ttttttcttc gtatcattga ataagtttga aaagccttga
//
LOCUS       DACDOA010000196         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDOA010000196 DACDOA010000000
VERSION     DACDOA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.202"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..972
                     /locus_tag="IWI39_002803"
     CDS             64..972
                     /locus_tag="IWI39_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9209824.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="IWI39_002804"
     CDS             972..1592
                     /locus_tag="IWI39_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209825.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="IWI39_002805"
     CDS             1596..2045
                     /locus_tag="IWI39_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209826.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
BASE COUNT          728 a          315 c          467 g          548 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caactgacaa agctaaactc gatggattag ccaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgt
//
LOCUS       DACDOA010000197         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDOA010000197 DACDOA010000000
VERSION     DACDOA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.203"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            493..714
                     /locus_tag="IWI39_002806"
     CDS             493..714
                     /locus_tag="IWI39_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209827.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IWI39_002807"
     CDS             715..972
                     /locus_tag="IWI39_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209828.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IWI39_002808"
     CDS             complement(1108..1710)
                     /locus_tag="IWI39_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9209829.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IWI39_002809"
     CDS             complement(1777..1932)
                     /locus_tag="IWI39_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9209830.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACDOA010000198         2055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDOA010000198 DACDOA010000000
VERSION     DACDOA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.204"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..972
                     /locus_tag="IWI39_002810"
     CDS             64..972
                     /locus_tag="IWI39_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9209831.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="IWI39_002811"
     CDS             972..1589
                     /locus_tag="IWI39_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209832.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1593..2042
                     /locus_tag="IWI39_002812"
     CDS             1593..2042
                     /locus_tag="IWI39_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209833.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          746 a          291 c          440 g          578 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaatgg
     1681 tgaagggaga taaaggcgat cctggccaag cagcaacaat taccatagga accgtttcta
     1741 gtggttcaac cgcttctgtt acgaatgtag gaacatcatc tgcagctaga tttaattttg
     1801 ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta
     1861 caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat ggaattgcag
     1921 ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa
     1981 ccgataaagt gaaacttgat ggattagcga atattacatt tgagaaagtg gggacggttt
     2041 aatggctgat attgt
//
LOCUS       DACDOA010000199         2023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDOA010000199 DACDOA010000000
VERSION     DACDOA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.205"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..633
                     /locus_tag="IWI39_002813"
     CDS             133..633
                     /locus_tag="IWI39_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9209834.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..>2023
                     /locus_tag="IWI39_002814"
     CDS             730..>2023
                     /locus_tag="IWI39_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9209835.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQT"
BASE COUNT          665 a          275 c          452 g          631 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa cta
//
LOCUS       DACDOA010000200         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDOA010000200 DACDOA010000000
VERSION     DACDOA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.206"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..78
                     /locus_tag="IWI39_002815"
     CDS             <1..78
                     /locus_tag="IWI39_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9209836.1"
                     /translation="NVVTGKENWNDLYHANEVLKIAEEK"
     gene            79..831
                     /locus_tag="IWI39_002816"
     CDS             79..831
                     /locus_tag="IWI39_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9209837.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            836..1783
                     /locus_tag="IWI39_002817"
     CDS             836..1783
                     /locus_tag="IWI39_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9209838.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          700 a          273 c          404 g          643 t
ORIGIN      
        1 aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa
       61 atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag
      121 gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta
      181 gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat
      241 acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta
      301 gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca
      361 ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt agatttgact
      421 tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta
      481 gataaagacg tctacttttc agttgtttct gcaagacgta atccctattt taatatggta
      541 aaaagaattg aaaatgaagt tactatagtt aataaatcaa gatacacatc taggcaacag
      601 acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa
      661 aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga ttatctaatt
      721 ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc taaattttta
      781 gaagattctt cggagcttca agatatctat aattatttat atcggaggta aaaggatgag
      841 ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca atcatttagg
      901 agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga
      961 tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga
     1021 gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc
     1081 agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat
     1141 tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc
     1201 ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt
     1261 gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt
     1321 atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc
     1381 gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt
     1441 aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag actttgttta
     1501 tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa
     1561 acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa
     1621 tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca
     1681 tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat
     1741 tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga
     1801 ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc
     1861 tgggatagaa gggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataatttgg
     1921 acagaacaga aagaagaaaa cctctttctg ttctgtttat ttggttaaaa atgttttgcc
     1981 atgacgaatc ataattcgta gtttgaaatt gtaaaagttg
//
LOCUS       DACDOA010000201         2000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDOA010000201 DACDOA010000000
VERSION     DACDOA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.207"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1154)
                     /locus_tag="IWI39_002818"
     CDS             complement(<1..1154)
                     /locus_tag="IWI39_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP9209839.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFL"
     gene            1175..1948
                     /locus_tag="IWI39_002819"
                     /pseudo
     CDS             1175..1948
                     /locus_tag="IWI39_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          604 a          399 c          327 g          670 t
ORIGIN      
        1 aataaaaatg cacgcaataa tctgtcaatt agcatattta ccttgattgg tattaccatt
       61 ttacctaaat ttttctcgat atatccactg aaatgaccta cgttaacact tgattttaat
      121 ttatcaaatc gactatattc attaatgttt acctcggtta agcgtaaatc caacatctca
      181 cgtaataatg cctcattaat attggttgaa tccactaaat cttggataaa atcgtcctct
      241 tcactaagct gatattcatt gaggtaatca gtaaattccc atccttcttt gataactaat
      301 tccccattat ttacatcatt tagaatcatc tcagcatatg gctgtaactc cactggtaat
      361 tgcgcaaatg tgcgatgtaa gtcgtttaac aatgattgaa ttaaattcga atcacgttct
      421 tcttttatat tcatgtattt tttgaagttt gtgtttaaat actcataatt aatacgacta
      481 cgactacctt caggtaattg gtaatcaatg tcatacgcta tactatcatt tccgtctcta
      541 ggttctctct ctatctcgct gtaacgttgt gccaagatat taaattcata ttctgtgatt
      601 actactgtta taggttcata atcctggatt tcataagtcg attttgccca tgtgaatccc
      661 tgtatcatcg ctgattcata acaatggatt agttgattat aaagatttac aaattgctta
      721 atttcccctg gaacctccgg taaatgactg aagttttcta tgccagaaat attaaatacg
      781 gacttaattt cttcgaatat atcgttcaac ttttctaagt tattatataa tttttcaaca
      841 aataaccctt ttgcttgttt gcctgagtat aactcaatag cctcttcaat gttctgttcc
      901 atcgtatgtg ctttacgata ataacgaatg gttccgaacg gtttttcagg accattaaga
      961 cgatttgtgc gtgaaaaagc ctgaattatg ttctgatatt caagtgactt atctaagtat
     1021 agggtgttga cccattttga atcgtaaccc gttaagagct gattaacaac tataacaaga
     1081 tctaattggg ttgcttcatc tactgttttg taaggattct tatgcgctgt gttttgcaat
     1141 agttttgtac acaagattgc aaagaaaagt acacaatgtt gccacttctg gttccatgag
     1201 ttttatttgt gttgatggga caaccaatca gctatttacg acccttgaag atcgcagact
     1261 ttacaaacta actcaatact tcctgcgctt tcctagaaag gcccgtttaa aggtcaagta
     1321 cctagtgatg gacatgaatg ccagctattg tcagttgctt aaaacggtct ttccaaatgc
     1381 ggagatcgta actgaccgct ttcatattgt ccaacacatc aatcgcagct tcaatcaatt
     1441 acgtgtccag atcatgaatc gttttcgaac ctcacactct gaaaatcaga agaagtaccg
     1501 tcggctcaag cgatattgga aactcttact caaagattcc acgacatggg aggcactcaa
     1561 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg ttaatgagct
     1621 gttaagctac aacgaagaat tgcgaactgc gtatcatttt tgtcaattat tacgctatta
     1681 tttcgttaaa cgagatacgg taggctttca agaaaccctt aaaacaattt catctacgtt
     1741 acctcagtcg tttaaaaaga aattcacgat cttccatcgc taccaatcgg gtatttcaaa
     1801 tgcctttaag gtgtcccact caaacggcgt cactgagggg ctgtataaca agatcaagct
     1861 gattaaacga attgcgtttg gttatcgcaa cttttataac tttcgggcac ggatctattt
     1921 acaacaaggt ctgatctttg aaaactaaaa aaatatgcgg cgcacagaac gcgccgcata
     1981 tcggactaag ttttattggc
//
LOCUS       DACDOA010000202         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDOA010000202 DACDOA010000000
VERSION     DACDOA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.208"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IWI39_002820"
     CDS             <1..969
                     /locus_tag="IWI39_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9209840.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IWI39_002821"
     tRNA            1159..1232
                     /locus_tag="IWI39_002821"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          668 a          349 c          376 g          600 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat gtcaagacgt
     1921 caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcgatctcta tctcgctggt
     1981 aagcctcgcg cag
//
LOCUS       DACDOA010000203         1953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDOA010000203 DACDOA010000000
VERSION     DACDOA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.209"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(355..1647)
                     /locus_tag="IWI39_002822"
     CDS             complement(355..1647)
                     /locus_tag="IWI39_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9209841.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          624 a          382 c          316 g          631 t
ORIGIN      
        1 tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt ttgaaatcgt
       61 tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga tagaataaga
      121 tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa aaaatacgaa
      181 aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca ttcatttaaa
      241 ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact atgaaaaccg
      301 taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt gaaatcagat
      361 aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg taaatcgcat
      421 acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca ccatagccca
      481 gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta aataaagtag
      541 ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc tgaagctgga
      601 gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac taccataatc
      661 aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac cagaataatg
      721 agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag aagaagcacc
      781 gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa tatagcgcaa
      841 tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa agatcgcttt
      901 tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat cttcgacacc
      961 acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg tttgatttaa
     1021 ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg tgtatacagc
     1081 atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa cgaatagaaa
     1141 ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa ggacagcgtc
     1201 agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt cgacggttgc
     1261 ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc cttcgatgtc
     1321 ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt cccggtctct
     1381 aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc catagccatt
     1441 tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta aatgattatt
     1501 caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca aagcatcttg
     1561 catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta acttttcggc
     1621 atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta ttgtagaaaa
     1681 agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg gtttatatta
     1741 cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt atctatttga
     1801 aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta ttatccatat
     1861 ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg ttaatttatt
     1921 tcatcccagt gcaaaatata aaaaagtaaa ata
//
LOCUS       DACDOA010000204         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDOA010000204 DACDOA010000000
VERSION     DACDOA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.210"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1167
                     /locus_tag="IWI39_002823"
     CDS             202..1167
                     /locus_tag="IWI39_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9209842.1"
                     /translation="MSKRGTIINEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPKEIETWWRLELQ
                     LRRGKATDWHEMVHESLDSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="IWI39_002824"
     CDS             1367..1570
                     /locus_tag="IWI39_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209843.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          643 a          282 c          432 g          589 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaataaaa tttgagtaaa cgtggcacga tcataaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat taccattgtt ggaaaactga aagaaaatat ttattatcat
      361 actcaaaatg atgtattgat tttagacttt gaacagttaa tgcgactgaa tgaaaataat
      421 ggttatgtta aatctgttgg tctaaatggt tggcaaattg tggataagca tgatgagaat
      481 attgcttaca ttgaaatttt gaaatatcaa gaaggtcaag ggcgaattga ctttaatccg
      541 aataaaatta atcagttttt agctagctct atgaagaatt ttatccatga tttatttttg
      601 gagccgcatt tttcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacccaat atcgggtggt tgatcctgta tcgtttaaac caatttatgg tcggtctggc
      721 aagttagaaa ctgcttattg gggcagtcgt gctagtgaac gtcaaattcg gatgtataac
      781 aaaaaattag aacaagaaag aaaacggaaa attgttccga aagaaatcga aacttggtgg
      841 cgtttagaat tgcagttgcg acgtgggaaa gccactgatt ggcatgaaat ggttcacgaa
      901 agtttggata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaatcaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat ttaaaatggc atattttgcg
     1681 ttctgagcgg ttttagcgtt tatttcgttt agttatcggc acaatcggtg aaacacgtta
     1741 gaggagcgaa aatggagcct gtaaggcgac tggtagcgga acggtcgtgt tagattgtgt
     1801 agccgatagc tgaacgaaat aaatgctggc agcgccattc tatttcggca ggaggaggct
     1861 caaggggttc ggggaatgaa attccctgag gtctttttat tttttctaga ggagtgctat
     1921 aatcaaagaa aatgagaaaa aagagt
//
LOCUS       DACDOA010000205         1901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDOA010000205 DACDOA010000000
VERSION     DACDOA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.211"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..501
                     /locus_tag="IWI39_002825"
     CDS             19..501
                     /locus_tag="IWI39_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209844.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            698..1588
                     /gene="pstS"
                     /locus_tag="IWI39_002826"
     CDS             698..1588
                     /gene="pstS"
                     /locus_tag="IWI39_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9209845.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          615 a          330 c          397 g          559 t
ORIGIN      
        1 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
       61 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      121 gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg
      181 attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      241 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctac gttttctttg
      301 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct
      361 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
      421 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
      481 tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa
      541 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
      601 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
      661 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
      721 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
      781 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
      841 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
      901 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
      961 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1021 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1081 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1141 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1201 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1261 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1321 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1381 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     1441 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     1501 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     1561 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     1621 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     1681 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     1741 tttattcgaa aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca
     1801 aatcgtgttg gtgaagacaa atttgaataa aacttagacc gatatgcggc gcgttctgtg
     1861 cgccgcatat ttttttagtt ttcaaagatc agaccttgtt g
//
LOCUS       DACDOA010000206         1899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDOA010000206 DACDOA010000000
VERSION     DACDOA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.212"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(435..932)
                     /locus_tag="IWI39_002827"
     CDS             complement(435..932)
                     /locus_tag="IWI39_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9209846.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(972..1781)
                     /locus_tag="IWI39_002828"
     CDS             complement(972..1781)
                     /locus_tag="IWI39_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9209847.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          545 a          358 c          334 g          662 t
ORIGIN      
        1 gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acaaggtaca taagaggttg
       61 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt
      121 tttacttggc tgttcttatt actgcagatg caagcttttg aataccgttt aaggaagagg
      181 ttgtgaaatc agcgttttga cctgagtatt agcttaacaa acggaaaaat agttcctcat
      241 attttgccgt ttgttaagct aataccgagg gtcagctgat tgaacaccat ttaagaaaga
      301 ggtcgtgagc ctgccgtttc actctgtgtc ataaaaaagc caatctactt tgatagaaaa
      361 agcacgtagg actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa tatcgtaaat
      421 gaagatagat atttttatga ttcgacgata gagacgttga aatctttcaa cagctctaaa
      481 gcggattctc cgtgttttct actggagact aacattggat caacaaaaaa gtctgcttca
      541 ggaaaagcac tctgcaggcc tatcagatta gagagtacac acaagtttgt ttcaatcccg
      601 actacttcga tctctttttg ttcttctttg ctttcaaata aagtttcctg gatttcagct
      661 aaagtttcag gtggaatcgt aaagtaatac tttttaatca ctcgttcatt ttcttgtgga
      721 gaaagaagag ggatcaattg caaatcttct cgttcttctt ctttgtcctt cagttcaact
      781 ggtatatccc gtgtgaagag aatatattca ttattttggc gtgctttagc taatctttga
      841 gcaatacgtt ctgccaattg atctgactct ggaatataaa attcgctgtt tggatcaaga
      901 atatgatttt gcatatcaat aataactaac attttctttc ctcctacatt catatcgtca
      961 ttttattctt tttaatagaa gttttttaaa atataagcaa caccagcttc ttcattcgtc
     1021 agtgttactt ggtttgcaat ttcttttact tgtcgctgtg cattccccat cgcaatactc
     1081 aacccgctgt aattcaacat agcaatgtcg ttttcttgat cgccaaaagc catgatgtca
     1141 ctcgattcga ttccaaggta atccgccaga aaaacaagtg aatgagcttt agtgatacct
     1201 gtaggcgtga tttctaagaa atgcggatca gagaatactg attggatacc atctgcttgc
     1261 agatctcttt gtagcttctg tagtttttct tcactcttat ctgttaatgc taatttaaga
     1321 tagctaagag aaggatcttc caacatattt aaaatttgat caaaagaatg gttctgtaaa
     1381 ttgaaatgtt cataataagg gtcatctttg actagatttg attcatcgaa tgtcagctga
     1441 tcatcttggg tttctcggct agcaatgatg gatattttcg cttcttgagc atagaagaag
     1501 gcttttctca gtgtatcgct atctattttt gaacgtctga gaactttcgc catttttcca
     1561 tccttcattt cagcaatcag tgaaccattg gaagaaatca aatagccacc gattcctaat
     1621 tcggaaaaaa tatctttagc agaaatgctg ttccttccgg tagcgacggt tacgattccg
     1681 ccattttcat caaatttccg aagtgcttgg atattttctt ctggtatctt tccatttcga
     1741 tcaagcaagg taccgtctaa atcaatcgct gctagtttca ttttatcttc ctactttctt
     1801 tttacatatc tattttcctg ttaccttaag attaaatgag aatttaaaaa aatgaaacta
     1861 acttgctttg tcattatgaa ttttttcaaa aaacattct
//
LOCUS       DACDOA010000207         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDOA010000207 DACDOA010000000
VERSION     DACDOA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.213"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="IWI39_002829"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="IWI39_002829"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9209848.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DACDOA010000208         1832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDOA010000208 DACDOA010000000
VERSION     DACDOA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.214"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..1240
                     /locus_tag="IWI39_002830"
     CDS             71..1240
                     /locus_tag="IWI39_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9209849.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1507..>1832)
                     /locus_tag="IWI39_002831"
     CDS             complement(1507..>1832)
                     /locus_tag="IWI39_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209850.1"
                     /translation="EDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVN
                     SKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKI
                     RTSHE"
BASE COUNT          651 a          314 c          287 g          580 t
ORIGIN      
        1 tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa
       61 ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat
      121 cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt
      181 ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat
      241 actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga
      301 ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg
      361 ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc
      421 ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc
      481 tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat
      541 ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat
      601 gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac
      661 ctctcattat gattcattga actttaccat attgactgga attttgatca aattaacaaa
      721 tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac
      781 aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa
      841 agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg
      901 gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc
      961 tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca
     1021 tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga
     1081 ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag
     1141 caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta
     1201 tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa
     1261 tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt
     1321 gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag
     1381 aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat
     1441 tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct
     1501 agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa gaagaacaat
     1561 tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg aatacctgat
     1621 tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc cttgttttag
     1681 tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga atacaggtaa
     1741 aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt tatttggaga
     1801 aaagctacta taaataactt gaccatcttc tg
//
LOCUS       DACDOA010000209         1826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDOA010000209 DACDOA010000000
VERSION     DACDOA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.215"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            709..1182
                     /locus_tag="IWI39_002832"
     CDS             709..1182
                     /locus_tag="IWI39_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9209851.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1191..1418
                     /locus_tag="IWI39_002833"
     CDS             1191..1418
                     /locus_tag="IWI39_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209852.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          592 a          309 c          274 g          651 t
ORIGIN      
        1 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
       61 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      121 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      181 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      241 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      301 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      361 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      421 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      481 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      541 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      601 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      661 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      721 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
      781 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
      841 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
      901 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
      961 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1021 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1081 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1141 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1201 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1261 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1321 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1381 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1441 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1501 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1561 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1621 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1681 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1741 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     1801 aagctcccat atatactgta aagcct
//
LOCUS       DACDOA010000210         1794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDOA010000210 DACDOA010000000
VERSION     DACDOA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.216"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..636
                     /locus_tag="IWI39_002834"
     CDS             133..636
                     /locus_tag="IWI39_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9209853.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..>1794
                     /locus_tag="IWI39_002835"
     CDS             763..>1794
                     /locus_tag="IWI39_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209854.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPS"
BASE COUNT          610 a          232 c          394 g          558 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagt
//
LOCUS       DACDOA010000211         1786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDOA010000211 DACDOA010000000
VERSION     DACDOA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.217"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            56..196
                     /locus_tag="IWI39_002836"
     CDS             56..196
                     /locus_tag="IWI39_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9209855.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(533..949)
                     /locus_tag="IWI39_002837"
     CDS             complement(533..949)
                     /locus_tag="IWI39_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209856.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(951..1232)
                     /locus_tag="IWI39_002838"
     CDS             complement(951..1232)
                     /locus_tag="IWI39_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209857.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
BASE COUNT          516 a          256 c          354 g          660 t
ORIGIN      
        1 aacgagaatt agatacatgg cttcaaggac ttgatgtgac ggaagtaggt gctgaatgag
       61 tgattcgata gaaaaaatga ccctaaaaaa acttgctgat gaattgggtg tatcgaaaaa
      121 tacggtgcgc aatcaactgg tcaattcggg atttgacctt gctcagtata aagaaaaaaa
      181 tgcagtggtc aattgaccga aatttatgtc tttgggtttt ggggttttaa aacgaaaaaa
      241 agggggcgat tttcgccccc tttgctctat gtgaaaaatt tgttatactt attttatcct
      301 taaaacatac caatcttttt cttatttagc tgctcgtacg agcagctttt tttacgatta
      361 tgaattcaaa tcatgtattt ataatttttt tgtttttatc tttttttctg ttatttcttc
      421 tgaaattcgt ttttttcatt caattggtta ctatcttatt gtaaatgttg ctattaagat
      481 ggtgatccta cccaaaaaat aaaccaggaa tttaaatctc ctggtttatt ttttatcttt
      541 ttagaaaatt tgttgggtcg aaatttttag aagtaaatgt ttgattggtt tctttttgac
      601 gattttttgg ttgatttcgt ttggagggat taagttgttt ttcaattttt tctttcgttt
      661 cttgatacac tttagaattt gtgccaataa gaatacctgt atcttttaga tataaatcag
      721 caaattctgc aatgatttcg gacatgatgg aaatttcttt tcccatagca tttagtttgg
      781 cttggttgct ggtagtcgta gtattggtat caggttcata actcatacat aaaaaacgaa
      841 tggcttccga gtaattgttg atatctggat ttctttcgat aatggaatct aaaacgtcca
      901 cgtgactggg ataaagggta acgtgttttt gaacagaagt ttttctcatg ttatcttcct
      961 ccgataagtg tttcaatgag ttggttgttt gtacgaacat attcattcag cgtttcagta
     1021 tttttttctt gaatagcgat aaatttttcg agtaataaat cgaattgatc tttcttgtaa
     1081 tgcataagtg cgtggtcaaa atcgttttcg attaggactt ttaagtagcg atttcgactc
     1141 cattttttgc cagttttatt tgtgagttct tggcaacgtt tgtctatttt tgcaataacc
     1201 tttgaatcga tcttccgaac taaaatgtcc atagaaaaac tcctttcgca tttgtgtata
     1261 atttatgtat atcatacgaa tatagtattt gtatagctgt tttttgctaa aatactatac
     1321 ataagctagc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa acgtgatagg
     1381 cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat gctctttata
     1441 aaatggtgat gtgcgacttt tttctgttcg taaaaataaa aaaagtttac gaaaatgaaa
     1501 ctaaactgaa cgattatgac gggcatgtaa tcaagatgaa atgattttat aagcgcgtat
     1561 tttgcgttct aagacgtttt agcgttcatt tcgtttagtt atcggcacaa tcgttaaaac
     1621 acgtaagaga agcgagaatg gagcctgtaa ggcgactggt agcgaaacgg cgtgttagat
     1681 tgtgcaagcc gagaactgaa tgaaatgaag agcggaagcg tgaaataatt tcggcaggag
     1741 gaggctcaag gggttcgggg aatgaaattc cctgaggtct ttttat
//
LOCUS       DACDOA010000212         1777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDOA010000212 DACDOA010000000
VERSION     DACDOA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.218"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..214
                     /locus_tag="IWI39_002839"
     CDS             <1..214
                     /locus_tag="IWI39_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9209858.1"
                     /translation="KPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKIS
                     NKGAHIETYEEVPREKVPEKALAIIG"
     gene            complement(364..436)
                     /locus_tag="IWI39_002840"
     tRNA            complement(364..436)
                     /locus_tag="IWI39_002840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Gln,seq:ctg)
     gene            complement(482..910)
                     /locus_tag="IWI39_002841"
     CDS             complement(482..910)
                     /locus_tag="IWI39_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209859.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(970..1728)
                     /locus_tag="IWI39_002842"
     CDS             complement(970..1728)
                     /locus_tag="IWI39_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9209860.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          543 a          317 c          336 g          581 t
ORIGIN      
        1 aaaaccagag gaagtaaatg aatatggtac agcaaggtat gtgcagaaag ctgctgatgc
       61 caatgggaaa gaacgaacgg ttgaatttaa tggtctttct gtgttaaaag aaaatcatta
      121 tttgaaaatt agcaataaag gagcacacat agaaacgtat gaggaagttc ctagagaaaa
      181 agttccggag aaagcattag caattattgg gtaaatcagt ttttgtatta agaatacgcc
      241 cgattttatt attcataatt taattatgta aactaaatac aagtagtaaa aataatacaa
      301 taatccccct gcaaaaagat tgttctttta cagggggttc attttttcag caagcacatc
      361 aaattgggat ggcagggttc gaacctacaa ttgcttgatt cagagtcaag tgccttacca
      421 gtttggctac atcccattta ttcaacaaaa taatcatagc actttcctaa aaaaacgcaa
      481 gttatttttc ttgatcggat agttgttgct ttactcgtaa aaggcggcgt actagtttct
      541 tttctaatcc agcaggaagg gcaatcagtt ctgctactga caagacggtg tccaataact
      601 gatattcttt gacaggttca ttcataatat gatagatgtt tgctaaacgt tcaatatatt
      661 caaagcgagt atactctgtt cgagcaatta gaaatttcaa taaatcggtg gctttctcgt
      721 actcttgatc tgcgtaataa acaagagatt gacgatacaa tttgatttcc agttcattga
      781 gggtagcagg tctgcgacgc tcgttccact cattaataga gactttacct tgaacatttc
      841 ccttgcagtc acggacaaaa ataatcggaa tttcatttgg atccattttt ttatgtgtga
      901 atttttccat tatctacgct cccttcaaag gtttggtatc ttgagtataa gggatttttg
      961 tttgaattac tagcttttct ccaaagttag catctgtcca cccattgcag tgaaaatcgc
     1021 cgaaaaatcg ttgatcggta ctgctcgatt ttttacacca tagggatcgt tgacataaat
     1081 attttctttg tcatatccag tgactacaca agcatgaaca agaggagtga ctcttacttc
     1141 tccattattt gtggtccaag tcataaaatc agtttcatct gggacttcaa aattgatagt
     1201 cacagaagtc cagacaggat ttcctttaga tagtgctttt ttgatatcat cgagttgagc
     1261 atcggttttt ggaaaagtgt gataatccgt ttggacgatt tgctgagcaa gggaggcaac
     1321 tgggggaaca tagacgacca tagcccagtc tccgccactg atatcaccaa caaatccttc
     1381 attaggatcg ccatgagtcc catctgcatt gaaaacaggg acgtaatcca attgctcagc
     1441 taactggttt ttagttgttt cataaccata aaaagataaa agcatagaaa gcgaagtgac
     1501 ttcacagcca ttttctaaag aaggagaatc aaactggctc tctaatggaa catctagtaa
     1561 aacttctgct ttctcttttg tctttttcgt tttttgtgtt gaactttttt gtgcagtgtt
     1621 cattgtatgt tctggagaat ggatatgtag gtaataactt gtaccgctta ataaaagagc
     1681 agctgtgccc cctagaaaca atagtttttt gaacgttttt ttcttcattt gaaatctccc
     1741 ttttcagctg tttatctttc ctattataaa tgtattt
//
LOCUS       DACDOA010000213         1751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDOA010000213 DACDOA010000000
VERSION     DACDOA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.219"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            275..>1751
                     /locus_tag="IWI39_002843"
     CDS             275..>1751
                     /locus_tag="IWI39_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9209861.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          642 a          246 c          312 g          551 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc t
//
LOCUS       DACDOA010000214         1703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDOA010000214 DACDOA010000000
VERSION     DACDOA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.220"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IWI39_002844"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IWI39_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9209862.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
     gene            769..1703
                     /locus_tag="IWI39_002845"
                     /pseudo
     CDS             769..1703
                     /locus_tag="IWI39_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          636 a          286 c          317 g          464 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggacataa agacggcaag aaatctctcg aaattcgaga atggacttgc
      601 cctatttgtc atactcatca tgaccgagat atcaacgcta gtatcaatat cctgaccgaa
      661 ggtctaaggc tacactctat gggattggct tagtggatag gctacaatta actagacaga
      721 aaaattaagg tgtgtagact agaagaaaac ataccaggag gaatttttat gtctaagaga
      781 acacgaagaa ctttttcaca agaattcaag caacaaatcg tcaatcttta cttagctgga
      841 aagccacgtg tagaaatcat tcgagaatat gaactaacgg cttcagcatt tgacaaatgg
      901 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
      961 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
     1021 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
     1081 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
     1141 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
     1201 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
     1261 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
     1321 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
     1381 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
     1441 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
     1501 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
     1561 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
     1621 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
     1681 gctgtccttt tgataatgcc gta
//
LOCUS       DACDOA010000215         1587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDOA010000215 DACDOA010000000
VERSION     DACDOA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.221"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            172..459
                     /locus_tag="IWI39_002846"
                     /pseudo
     CDS             172..459
                     /locus_tag="IWI39_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="IWI39_002847"
     CDS             473..904
                     /locus_tag="IWI39_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209863.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="IWI39_002848"
     CDS             906..1043
                     /locus_tag="IWI39_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9209864.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1080..1373
                     /locus_tag="IWI39_002849"
     CDS             1080..1373
                     /locus_tag="IWI39_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209865.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1370..>1587
                     /locus_tag="IWI39_002850"
     CDS             1370..>1587
                     /locus_tag="IWI39_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9209866.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGG"
BASE COUNT          579 a          231 c          339 g          438 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccattatgca ttacagacga agaagcagat aaaatcatta
     1021 ataaagataa gagcgcatct taattgatgc gtttttattt aaggtaaagg agttgtcaca
     1081 tgattaattt aggggaatgg ggaatgatag caggatcaat aaccgctatc gtttctttga
     1141 ttttattagt aattagaccg attgctgcat ctttctcgaa aattactgag actctttcaa
     1201 aagtaagcca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
     1261 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat acagaaaaat
     1321 tggtggaaca cacgcaacaa atcaaaactt tgtttaggga gagaagaaaa tgaataataa
     1381 aacgttcgaa gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt
     1441 ggggttagtt ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac
     1501 tggttttggc gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt
     1561 ctcggctgaa agcgaagaag gaggaaa
//
LOCUS       DACDOA010000216         1583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDOA010000216 DACDOA010000000
VERSION     DACDOA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.222"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..569
                     /locus_tag="IWI39_002851"
     CDS             <1..569
                     /locus_tag="IWI39_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9209867.1"
                     /translation="LIYGFFDDKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGD
                     CVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAII
                     NTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGS
                     TIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            520..>1583
                     /locus_tag="IWI39_002852"
     CDS             520..>1583
                     /locus_tag="IWI39_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9209868.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHD"
BASE COUNT          560 a          217 c          332 g          474 t
ORIGIN      
        1 agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg aaatatcttg
       61 ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat aaatttattg
      121 tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat atgccaagtg
      181 agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt tcaattggtt
      241 atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg
      301 ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac tatgttcatt
      361 tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact caaataggaa
      421 ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga
      481 gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca gctaaaatta
      541 agaaagaagg aattaatttt gaattctaga attttattat caagtcctca tatgggagga
      601 acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc attaggtgaa
      661 aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg cggagcttct
      721 gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa tgttggaaaa
      781 aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc tattttatat
      841 caaggggctg taccagtgtt cattgactca gaacctaata catggaatat gagccctgtt
      901 gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa ggcaatcatc
      961 gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat tgcaaaccaa
     1021 tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta taagggaatt
     1081 tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa gatcatcaca
     1141 acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca ttcaagattt
     1201 ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat aggttataat
     1261 taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt attgaatgat
     1321 agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc tggaattgat
     1381 agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt gtctacttta
     1441 acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa actaaatgaa
     1501 aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt atttcaaggg
     1561 gtaaaaatgt attaccatga taa
//
LOCUS       DACDOA010000217         1567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDOA010000217 DACDOA010000000
VERSION     DACDOA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.223"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..403
                     /locus_tag="IWI39_002853"
     CDS             5..403
                     /locus_tag="IWI39_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9209869.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="IWI39_002854"
                     /pseudo
     CDS             387..1081
                     /locus_tag="IWI39_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          594 a          224 c          309 g          440 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1501 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1561 ataaaaa
//
LOCUS       DACDOA010000218         1546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDOA010000218 DACDOA010000000
VERSION     DACDOA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.224"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(311..925)
                     /locus_tag="IWI39_002855"
     CDS             complement(311..925)
                     /locus_tag="IWI39_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9209870.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          509 a          300 c          239 g          498 t
ORIGIN      
        1 tttcatcaac gtgtattaag agacaagaaa gaatccatat ggattctttc ttgtctcttt
       61 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
      121 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      181 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      241 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      301 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      361 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      421 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      481 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      541 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      601 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      661 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      721 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      781 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      841 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      901 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      961 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
     1021 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1081 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1141 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1201 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1261 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1321 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1381 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1441 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1501 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctc
//
LOCUS       DACDOA010000219         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDOA010000219 DACDOA010000000
VERSION     DACDOA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.225"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            20..763
                     /locus_tag="IWI39_002856"
     CDS             20..763
                     /locus_tag="IWI39_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209871.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(800..1297)
                     /locus_tag="IWI39_002857"
     CDS             complement(800..1297)
                     /locus_tag="IWI39_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209872.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(1353..>1509)
                     /locus_tag="IWI39_002858"
     CDS             complement(1353..>1509)
                     /locus_tag="IWI39_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9209873.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          477 a          271 c          250 g          511 t
ORIGIN      
        1 aatcaacaaa aaaacgcata tagtagtctc aaaactccca agaaatgagg actatttaac
       61 aaactacgat ggttacgtcg tgacaactga acattttgac ttagccaatc ctgttactaa
      121 agtcgatgat atccccgatt atgaaatgta cagtcaaaca atcgatagct taaataagcg
      181 ttttggcaat cgtgttttaa aaggcatcaa aatcggttac atcgcttctg aaaaggatcg
      241 gatcatcgac tatttagccg ataaagatta cgatttaaaa ttactcagtg tgcatcataa
      301 tgggcaattt gattatttag atgacgaagt caaagatatg gatcctgcta ttgtgattcc
      361 tcagtatttt gcacaacttt cagaagcact agttgttata gaagcagatg tctttgctca
      421 ttttgactat ggttttcggg tatttggtct atccgttgct gaatttaaac aatatgaagc
      481 gcagttttta ccgattctag atcaagtaat caaaaataaa ttggcattcg aattaaatgc
      541 taagagcgct tacttgtatg acaatttagc gttatatgaa tatgtcatcg atttatatct
      601 ttcacgtggt ggtaccttgt ttagtgttgg atcagatggg cattaccttg aacatttccg
      661 tttccatttc gatgatcttt ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat
      721 ttatcaaaaa ggaaagcgaa ttatggttcc attaccagtc taaaaaaacg tcctcagcat
      781 caaaagtgag gacgtttttt tagatttctt tttctaaaat cggacaaata cgatcttgtt
      841 ttcctaaagg aaaatgtttg atcccgattt ttttaaatcc atgacgttca taaaaacgaa
      901 tacctgcatg atttttttca taaacagtca aacgtaaata acgtttttgt ttcgttttag
      961 cataacgatt cgcttcatcg agtaaccgtt gccctaaccc tttgttttga taatctttga
     1021 gcagatagag ccgttgaatc tctatagcat ctttttcatc attgactttc atataaccaa
     1081 tgagtatttg atgatcctta ataccaataa atttactggt tgactgtgcc aattctactg
     1141 ccaatatttc agttgtcaat tgtgtctgta aatatccttg catatcaagt ggatctgcgc
     1201 catcgataaa cgattcataa aaactgacaa cacttacaac ttgtaaagca tctagatcat
     1261 cataatctaa atacgctagt gtgatttgat tgttcatcta actcctcctt ttgacatagt
     1321 atactataaa tcacccgtgg tgctagttaa ttttaatatc ctaaagaata tctcaaagtt
     1381 ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt aagccagtaa tatgctttca
     1441 aatctactct ctagcccttt aacagaacgt gagttgaaat tttgacaacc taatttttct
     1501 aaggaagaa
//
LOCUS       DACDOA010000220         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDOA010000220 DACDOA010000000
VERSION     DACDOA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.227"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..1415
                     /locus_tag="IWI39_002859"
     CDS             75..1415
                     /locus_tag="IWI39_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9209874.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          321 c          284 g          388 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
      961 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     1021 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     1081 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     1141 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     1201 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     1261 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     1321 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     1381 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     1441 ttgaatttct tcacc
//
LOCUS       DACDOA010000221         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDOA010000221 DACDOA010000000
VERSION     DACDOA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.228"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..1361
                     /locus_tag="IWI39_002860"
     CDS             66..1361
                     /locus_tag="IWI39_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9209875.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          446 a          283 c          267 g          429 t
ORIGIN      
        1 tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat
       61 tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct
      121 atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca
      181 aaggaactct tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta
      241 ggatcattcg ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc
      301 gcacttatct ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg
      361 tagcggatac ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg
      421 tcattgctcg cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt
      481 ccacttcttc ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac
      541 agactttgcc aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg
      601 caatgagttt tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc
      661 gacgcttgcc ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg
      721 ttcaattgat tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc
      781 cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct
      841 tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag
      901 ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa
      961 actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg
     1021 ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca
     1081 ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca
     1141 ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac
     1201 agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc
     1261 acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaactac
     1321 gaattatgat tcgtcatggc aaaacatttt taaccaaata aacagaacag aaagaggttt
     1381 tcttctttct gttctgtcca aattattcta agatttctct ttacc
//
LOCUS       DACDOA010000222         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDOA010000222 DACDOA010000000
VERSION     DACDOA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.229"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWI39_002861"
     CDS             complement(150..1337)
                     /locus_tag="IWI39_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9209876.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDOA010000223         1383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDOA010000223 DACDOA010000000
VERSION     DACDOA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.230"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IWI39_002862"
     CDS             123..1292
                     /locus_tag="IWI39_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9209877.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          496 a          225 c          240 g          422 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ata
//
LOCUS       DACDOA010000224         1363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDOA010000224 DACDOA010000000
VERSION     DACDOA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.231"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..495)
                     /locus_tag="IWI39_002863"
     CDS             complement(<1..495)
                     /locus_tag="IWI39_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9209878.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLS"
     gene            complement(485..>1363)
                     /locus_tag="IWI39_002864"
     CDS             complement(485..>1363)
                     /locus_tag="IWI39_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9209879.1"
                     /translation="EVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWI
                     DLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIV
                     NSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDT
                     IKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKT
                     PSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTR
                     QMKRDAGAVGGTLYAT"
BASE COUNT          434 a          244 c          163 g          522 t
ORIGIN      
        1 actcaattta ataagcggtt ttgaagaata cttctctggg ttataaattt caaaagtttc
       61 tgtttgtcga atagtaaatc taccattgta attttcctta aatggtcgaa tactcactgt
      121 aaattcaaaa gggacaatat ttcctgtttt tcttgttcct ttaaattcag gtccttctat
      181 tactacagct tgataaatat attgttcatc atagtaaaga ataaaatcac tataagacga
      241 catatctaac cattcagtga ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc
      301 tttatagtaa cactcaatct ttctagtcac atttttgtaa tagcctttat caataatgat
      361 agaatcattt ccttctcgct ctctcaattc aattactcga ctagctgaaa cagaagcagg
      421 cctgttttga atatacacat taaattcaga agaataatgt tgattaataa aaaattgtcc
      481 tctcttaagt tgcatataat gttcctccta ctgcaccagc atctcgtttc atttgtcttg
      541 tcaattctgt tttaattttt gtggtaatct tccgaaccat tgaatcaggt aaatctccat
      601 aaacatttaa atgtaaatga atttcttttg tttcactagc agatatattt tgctttgttt
      661 gtgtaattgg taggtgactt attccattta ctacaggttg aacagacggt gtttttacta
      721 aatttgtcat ggtttgatct aacttacttt gctctttatc aataccaacg ataatacctt
      781 gaacaatatt tttaccaacc atatcacgca tccatcttga tggagaatga atatctaaag
      841 ctcctttaat ccaatttttg atattacctg caataccttt gatagtatct ttcaaagcat
      901 tccacttttc ttttacacca tttataagtc catcaatgat gttcttgcca atttcaaata
      961 aattgacttc ccttaacgaa ttaaagattt cttttacccg attaatagca tttgaaacac
     1021 cattttttag atttgtccat gcattttcag cagaattaac aattccagta acaatattcc
     1081 agaaagagtc cttaatgtta ttccatgtat taatcatggt attttttata gaaatccacg
     1141 tattgtatgc cgtatcttta atatttttcc aagtatcttt gaaaaactgt ttaatattat
     1201 tccaagtagt aatggcatta tattttaaat cgatccaagt ttgaataata gaaaacttca
     1261 attcaatcca tttctgaatt gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat
     1321 actttatgtt taaccaaatc gatttagctt gatttaccac ttc
//
LOCUS       DACDOA010000225         1360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDOA010000225 DACDOA010000000
VERSION     DACDOA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.232"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..>1360
                     /locus_tag="IWI39_002865"
     CDS             78..>1360
                     /locus_tag="IWI39_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9209880.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          377 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg
       61 agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata acagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg ttggtcgtcg
      181 atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact gtggtagatg
      241 gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc cgttttgaac
      301 aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc tgtaaaaact
      361 gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca atcgcaaatc
      421 atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt aaaagctatt
      541 taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa tttcgttcgc
      601 atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca ggaaaattaa
      661 tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta ggttgtacca
      721 atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc cagctcacca
      781 aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc aaacacatga
      841 atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa gccggacaga
      901 aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gtttccgagc accaaaatac ccatttctta
     1021 ctgaagcaat gatgattgat cgattgcttg agttttctcc gcccctaaag gaggcgtatc
     1081 ccttttttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1141 tattggcaga acttcccgaa acgttagatg acagctttcg ggaaaagctt caaaaccttc
     1201 tgacctatga agaaggcatc accaacgcaa tgatctatcc ttattccaat ggaaaaatag
     1261 aagcgaagaa tacccacata aagacaatga aacgagtatc ctacggattt aaatcatttg
     1321 agaacatgag aattagaatc tttttgatca atcaattaat
//
LOCUS       DACDOA010000226         1346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDOA010000226 DACDOA010000000
VERSION     DACDOA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.233"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            731..943
                     /locus_tag="IWI39_002866"
     CDS             731..943
                     /locus_tag="IWI39_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209881.1"
                     /translation="MKNRLEELRKQRGIKREDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            933..1328
                     /locus_tag="IWI39_002867"
     CDS             933..1328
                     /locus_tag="IWI39_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209882.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          472 a          200 c          231 g          443 t
ORIGIN      
        1 actcttatat atttccgaaa attatcttga agatttctat tggtatggag aatatccatt
       61 tcattgccaa taaggatata aattctcaaa ataacaatcg atgtctgtat taaacaagcc
      121 tagaatggta aatctgattc gccaaaacca ttggattgta tcgatttgct ttacgcgaac
      181 gccacctacc cattttataa aaataaaatg ggcaaaagat ctttcttttt gtcatacaaa
      241 aagaagaaaa aaaagagacg attttcttct tccaaaatcg tccctctttt ttctaaaacc
      301 atggatgaac gaacaaaaaa tctctcgtat tttgtttgtt catccactaa aaatatgcga
      361 acagtatggt ttcattcaat cctgttctta caagtcaaaa gcatcgaatc gtccttggac
      421 gattcaacaa aaaaagtagt cgtttttgga tgactactaa gttctaaaca ttcattaccc
      481 aaaatcaatt tggtctaaca tcgtttctcg actggcttga tctaatcggc tcttgacagg
      541 gctttttctt ttctgttgat agcagataag aggaaataag aataagtgtg ttcatgtgtc
      601 ataaaaacag taactaaaac attaaaatca aaatttatat ggaaagcttc cttgacattt
      661 tgtgctcgca ggagtattat aaaagcacga aaggaaagta aactttccat tttttagaaa
      721 ggagcatgtt gtgaaaaatc gtttggaaga attacgaaaa caaagaggaa ttaaacgaga
      781 agatttagct tctgctcttg aagtatctcg tcaaacaata ggttctttag aaaatggacg
      841 ttataatccc tctattatat tggcatttaa aattgcaagg tactttcaaa tgagcattga
      901 agaaattttt atctatgagg aggaaagtga agatgaaaaa taagggtcag atttggtata
      961 tcggttatat tgtttcgttg tttttgattg tgacaatttt ggttacagat tttcctaaaa
     1021 tggtagatat tggacttgct attttattca gtgccgtttt tggcgtttct catgttcaaa
     1081 ttttgcatga taaaatgatt aaaaatgatg cagactacaa aatcaatgtt atggacgaac
     1141 ggaatatcat aatcaaagaa aaagcgggaa atataacaaa tatgtttaac acaatactgt
     1201 taggggtggc aacagttata tttatttgtt tagactacat cattccagct attattgtcg
     1261 gaagtatcat tgcaatccag ccgattattt taatcattat cagtagtgtg cttgagaaaa
     1321 aaatgtagaa ataaaggttc atacaa
//
LOCUS       DACDOA010000227         1335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDOA010000227 DACDOA010000000
VERSION     DACDOA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.234"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            409..741
                     /locus_tag="IWI39_002868"
     CDS             409..741
                     /locus_tag="IWI39_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209883.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            651..854
                     /locus_tag="IWI39_002869"
     CDS             651..854
                     /locus_tag="IWI39_002869"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9209884.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1038..1193
                     /locus_tag="IWI39_002870"
     CDS             1038..1193
                     /locus_tag="IWI39_002870"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9209885.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          480 a          235 c          233 g          387 t
ORIGIN      
        1 ttctataatg attcatagaa tctaaaacga atgattttaa ttattaacgt tttttaattc
       61 gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt
      121 acgaagtggg caggaggtcg aatccagaac tgggccatgg tacttcaacg aggacttctc
      181 ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat
      241 gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa
      301 ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga
      361 tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt gaaaaaaaca
      421 tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc
      481 aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa
      541 tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca
      601 gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg
      661 aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg
      721 aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat
      781 ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaaa
      841 tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgacg gatattactt
      901 acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta gcaactcgta
      961 ttctacgtta ctatatggtg gattcacaca tgaaaaaaga gattgtcgtc cgtccattac
     1021 aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc cagcatcggt
     1081 cttttattta tcaagagctg ttgaccgacc accacatcac acattcaatg agccaaactg
     1141 ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa
     1201 tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa gattatttag
     1261 aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct tgacaatttg
     1321 aagcgaagaa aataa
//
LOCUS       DACDOA010000228         1326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDOA010000228 DACDOA010000000
VERSION     DACDOA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.235"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            107..979
                     /locus_tag="IWI39_002871"
     CDS             107..979
                     /locus_tag="IWI39_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9209886.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          408 a          234 c          308 g          376 t
ORIGIN      
        1 aatagtagtt atttacttgt catgttacta aataaaaaaa taatttaaat atgatatata
       61 gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc tatacggtgc
      121 aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa
      181 ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag tcattgactt
      241 cttcaaacaa taccaagcag atttagcagg aattgggatt ggctcatttg ggcctatcga
      301 tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat tggcatggca
      361 aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt cttggacaac
      421 agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag gattgagcag
      481 tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag aagggcgctt
      541 tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc atccaaaaga
      601 tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg ccgctggacc
      661 cgccatcgaa aaccgcttag ggaaaaaagg acaagaggtt gcagaagatg atccttattg
      721 ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga tgttttctcc
      781 agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca
      841 cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat
      901 cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat tggctcgaga
      961 agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag
     1021 atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt ttttgtttta
     1081 cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg aaatttcgat
     1141 agccgtaggc cacttgtatt gtgcaatagt tttttacaaa aaaaaggggg tgcggatatt
     1201 tttttggacc tttttaacaa ataggttctg ttgcaaagtt ttaaatctac tatcagataa
     1261 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg agtcatttta aaggaaagca
     1321 atttca
//
LOCUS       DACDOA010000229         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDOA010000229 DACDOA010000000
VERSION     DACDOA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.236"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            588..974
                     /locus_tag="IWI39_002872"
     CDS             588..974
                     /locus_tag="IWI39_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9209887.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1072..1263
                     /locus_tag="IWI39_002873"
     CDS             1072..1263
                     /locus_tag="IWI39_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209888.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          498 a          202 c          192 g          430 t
ORIGIN      
        1 cacttatcgc tttcatgtat attccaccgt aaaaagtaca ttttatcacc gagaactcat
       61 catttttcat catgatgaat actatttaca tatatctagt tcaaagacaa taattattat
      121 gatccgttat tatctaagca ctatacagat aataggaatt tttcgtttct aaatactcca
      181 atttgggaat ccaataaaaa ttttcgttca aatacgtgtc tattcgaact cttttctagt
      241 tgttaattat gaagggggaa gaaataaaaa atacaatcta tacacgaata agaatattat
      301 ccaaaaggag gttttagaaa agatttatta tacgtaaaaa gctcaagaca ttttcttagc
      361 cataaggaca tgtttgatat ttaagaagat atatgaattt tcttttctaa gtataaagat
      421 aactatccat tatcaactaa aaaaacattt ttgaaaataa acatcagcag tcctatcaat
      481 gcatgtgagc ttatagaagt tattcgcagc gcagcataat tgcattagct tcttttcgtg
      541 ttttttgcct cttttccctt taaatcttaa tggaggaggt gtgcaatatg tcagataacg
      601 acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa ggtcattttg aacaccgcta
      661 atacttatta taaaaaacaa gaagaaattc aaaaacatga acaacttgat caactcccac
      721 catatttgtt tactaaagat actataaaat ttaaacaacc gattaatgtt ttaaatgtga
      781 ctctccttgt tgaagatata aaattagcaa aaatccttcc tttgttgaaa gaacctgaac
      841 aaatttttct tatcgaaaaa tttatcttta ataaaactga taaagaaatt ggaaaggttc
      901 tgggtattac aagacagggt gcgacaaatt taaaacatcg actttacaaa aaaattcaaa
      961 aaatgctggt ttaatatctg cataataact aattttgtat gcttctttgc tcgtcctcct
     1021 ccttccccaa aaaataaata gattataagt tcatgggaag aaggtaaatt tatgaatact
     1081 ttaaaagaag atttcatttt agcaaagaat ggaaatgaag aagcagttgt ggccattctc
     1141 aaaagattta gcgctttggt ccataagcaa tcctggagaa ctggaaaata tgatcaagat
     1201 tgttatcaag aatgtatgct tgcaatatat ctagctatct cgaaattcga aattaaagaa
     1261 taactcaact aagggagtct gtgacataag taaaaagata gtttcaaatt tcgaacaata
     1321 aa
//
LOCUS       DACDOA010000230         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDOA010000230 DACDOA010000000
VERSION     DACDOA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.237"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..805
                     /locus_tag="IWI39_002874"
     CDS             <1..805
                     /locus_tag="IWI39_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9209889.1"
                     /translation="NTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYNPEGQK
                     VTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVE
                     VTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTV
                     KVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNR
                     GKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            939..>1316
                     /locus_tag="IWI39_002875"
     CDS             939..>1316
                     /locus_tag="IWI39_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9209890.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLA"
BASE COUNT          528 a          173 c          265 g          350 t
ORIGIN      
        1 caacacacca ggtaattacg aaggggttat cgaagtaacg tatccagatg gtacgaaaga
       61 tacagtgaaa gttccagtag aagtgacaga caatcgttcc gatgccgata aatacaatcc
      121 tgaaggacaa aaagtgacta cagacttaaa taaagagcca gacgcatctg agggaattaa
      181 gaacaaagaa gatctaccaa aaggaactac ttatacttgg aaagagaaag tagatgttag
      241 tacagctgga aacaaaaaag ggattgtagt agtaacatat ccagatggat ctaaagaaga
      301 agtagaggtt actatttctg tagaagataa aaaagcaccg aataaacctc aagttgatcc
      361 tattacagag ggtgaccaaa ttgttactgg taaaactgaa ccaaatgcag aggtgacagt
      421 tacattacca aacgggagtc aataccatgg gacagctgat aaaaatggta attttacagt
      481 taaagttcct aaattagagg caggaacaaa agtgatagta actgcaactg atgaatctgg
      541 taatactagt gaacctacta atgtagttgt ttcttcaaac gaaaaagaca gtgaaaaagc
      601 cgttagtaaa gataataaga ctgacaatca aggtagcaag caaaacaaaa atagaggcaa
      661 aagcagtcct caaaagcagt cctcaaaagc ttatcctaag acgggagaga ttgatagtaa
      721 tatctttact attagtggtg gtttaatcct gttaggaact ttagggttat tagggtataa
      781 aaatcgaaaa aaagagaatg aatagaaaat caatttctta aatcttctct gaaaagagga
      841 agaatctttt tcttcctctt tttgtttttt attttgggag ataggttgtt agttaaaaat
      901 gaagatactt ttcatagaag ggaaaagaat gtgattaagt gaaaaaaata gtaattattg
      961 gatcaaacca ttcggggcta gctgcagctc gatttctaaa cttatgtgat tcaaattatg
     1021 aagttattct gatagataaa aaatcagaaa ttagttactt agaatgtggg acatcgttgt
     1081 tcataaaaag agaagttagc aatttagaag atctttttta tcaaaataga agagaactaa
     1141 attctaagat aaaaaagttg tttttggaga cagcagttga caaaattgat attcaaaata
     1201 aaaagattat ttgtgtaaaa aaagatggca caagattttc ccaagaatat gacaaactta
     1261 tactagctac aggttcaaag caaattatgt tagatgtacc ggggaataat ttagct
//
LOCUS       DACDOA010000231         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDOA010000231 DACDOA010000000
VERSION     DACDOA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.238"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWI39_002876"
     CDS             200..742
                     /locus_tag="IWI39_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209891.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="IWI39_002877"
     CDS             898..1272
                     /locus_tag="IWI39_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9209892.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DACDOA010000232         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDOA010000232 DACDOA010000000
VERSION     DACDOA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.239"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWI39_002878"
     CDS             <1..1244
                     /locus_tag="IWI39_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9209893.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDOA010000233         1270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDOA010000233 DACDOA010000000
VERSION     DACDOA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.240"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(79..369)
                     /locus_tag="IWI39_002879"
     CDS             complement(79..369)
                     /locus_tag="IWI39_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209894.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(487..747)
                     /locus_tag="IWI39_002880"
     CDS             complement(487..747)
                     /locus_tag="IWI39_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209895.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(884..>1270)
                     /locus_tag="IWI39_002881"
     CDS             complement(884..>1270)
                     /locus_tag="IWI39_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9209896.1"
                     /translation="MFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYKLTADMPGCD
                     KEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPNVDEEKITGT
                     FKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          474 a          217 c          188 g          391 t
ORIGIN      
        1 acttcaaaaa agataacacc caactttcaa aaatatgttt tggaagttgg gacaaacgag
       61 ttaatacatt ttaatacatt attttttatt caaaaaaaat agcacaatgg caaagatcgt
      121 aagagagatc ataagcccca atgaatgact aaaaagaaaa gcagaatcac atgccatatc
      181 atcccatatt tcttcaagaa aataaataag cgggaaaaag ggaaggaata gtagaacatc
      241 gatccagcac catttttttc ttcttttgaa ataacataaa acaataaaca tgaacgtgca
      301 gggaagtaaa aacaaagctt tactcataac taacaaaata ccagctcctt ttattttttt
      361 ctcccccaca ttcaaagagt aacaatggta tgtacttaat aatagaaaaa tggatcaact
      421 tgtatgaata acagctgaaa agatctgcac caaataaaaa acttccagcc aaaactgact
      481 ggaagtttat ttttttctga aatattctaa cacaaatatg aaaaagatgg ccgaagctaa
      541 taagccgatt tcgtagcaaa acaatgagat accagagtcg taataataaa atcctctaat
      601 cttatggata aaatagatca tcggacaagc agataagagt aagtatttgt taggcctttt
      661 gaataagtaa aagaaagcac tggtcattat gataaatggt atccaaaaca aaagtttact
      721 taagacaatc aaatgatcaa ctcccatatt ttttaccaag agagatcata acatctttat
      781 cgtaaaaaca aaatattttt ttgctcattt tgaaaattta aacatactcg ttcttatcca
      841 aatatataaa aatcgcccct cattcatcga gaggcgataa gttctaattt aactcaattt
      901 tttttgtttc ttttggctga taagctgttt taggtaatac caatttcagc acaccgtttt
      961 taaaggttcc agtgattttt tcttcatcaa cattaggaag atagaatgaa cgtttgtaag
     1021 agaccgaatg tctttctttc cgtacatagt tgccatcttc tttatcttct gtatgggact
     1081 catgattggc ggagatcgtc aaagtattat ctgaatattc aactgtcgtg tcttctttgt
     1141 cacagccagg catatcagca gttagtttgt attctttttc attttcgact aaatctactt
     1201 ttggataact tgaaacacct aaaaaatcat tgaatgctgg actaaacacg tcattgaaat
     1261 cagggaacat
//
LOCUS       DACDOA010000234         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDOA010000234 DACDOA010000000
VERSION     DACDOA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.242"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWI39_002882"
     CDS             <1..1076
                     /locus_tag="IWI39_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9209897.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDOA010000235         1186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDOA010000235 DACDOA010000000
VERSION     DACDOA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.243"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..1098
                     /gene="xerS"
                     /locus_tag="IWI39_002883"
     CDS             13..1098
                     /gene="xerS"
                     /locus_tag="IWI39_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9209898.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          478 a          195 c          184 g          329 t
ORIGIN      
        1 aggatgaata acatgaaaaa tacagaaata tatcaaaaaa tagatacaat catacttcat
       61 atgcccgatt atataaaaga ttttgttcaa gatcgtgagg ataaagatca atcacctcga
      121 actactttag aatatctaaa aaattataag ctcttttatg aatggcttct gagtgagtct
      181 atcgttcccg atacttcaat cacctcgata agacagttaa ctgcctatga tctaaacttg
      241 tacaaatctc atttgaaacg tcgcacaaag gaaaatgtaa aaaaagagac aacaaaaact
      301 aagctcaaag ataataataa cttaggtcta tctacttcta ctatcaatag aaatatcaca
      361 gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt aggaaaacca
      421 tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac tacgttagct
      481 gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca aaattatcta
      541 gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat ctattatcaa
      601 cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg attaagactc
      661 tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa tgtggtagta
      721 actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat ggaatattta
      781 gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga aaaagcttta
      841 tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat cgaacggatg
      901 gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt acgtcatact
      961 ttggctacta gactctatca acagacaaat tcattggtat taactgccca acaattggga
     1021 catacaagta caaatacaac aactttatat actcatatag acaatgcagc tacaattgat
     1081 gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct gtctctaaaa
     1141 ttagataaaa gcccaacttt aaaggcaagc ttcattatca ttttta
//
LOCUS       DACDOA010000236         1160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDOA010000236 DACDOA010000000
VERSION     DACDOA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.244"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IWI39_002884"
     CDS             14..1141
                     /locus_tag="IWI39_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9209899.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DACDOA010000237         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDOA010000237 DACDOA010000000
VERSION     DACDOA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.245"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            237..>1157
                     /locus_tag="IWI39_002885"
     CDS             237..>1157
                     /locus_tag="IWI39_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9209900.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKR"
BASE COUNT          398 a          183 c          249 g          327 t
ORIGIN      
        1 aggagtgcta taatcaaaga aaacgagaaa aaagagcaaa ataaaagccg aacggtgttg
       61 gaagcaccct tcggctaatt acggaaacta ctcacctagt tccgtaactc aaattcatta
      121 tacgaatatt tttaggtttc ttcaagctag agatttttgg atatggattt ttagagttac
      181 gtgaactctg gaaattcttt ccaaaaatct cttttttctt tgaggaggaa caaaatttga
      241 gtaaacgtgg cacgatcata aatgagtggg aaaaacttaa ccgcatatct aacaggcggt
      301 taaatgcaca aactatgtgc acccccttaa aaatgcaatg gtcaattgac cgaattacca
      361 ttgttggtac attaagggaa aatatttatt atcacactcc ccatgatgtg ttgattttag
      421 attttgaaca gcttatgcgt ctaaacgaag gtaacggata tttaaaagcg gttggaaata
      481 atggctggca attactagac caacatgagg aaaatatagc ttatattgag attcttaagt
      541 ttcaagaggg aaaagggcgg attgacttta accccaataa aattagccaa tttctggcgg
      601 gctcgatgaa gaactttatc catgatttat ttttagaacc acatttctca cgtgcagata
      661 ttgcttgtga cattattgac gtgccagatg atttcattac ccaatatcgg gtggttgatc
      721 ctgtttcttt caaaccaatt tacggtcggt ctgggaaact ggaaacagca tattggggaa
      781 gtcgtgctag tgaacgacag attcggatgt ataacaaaaa attggaacaa gaaagaaaac
      841 ggaaagttgt tccgaaagaa atcgatactt ggtggcgact agaattgcaa ttgcgaaggg
      901 ggaaagcaac tgattggcat gcaatggttc atgaaagttt agatagcttt gcaagtcctc
      961 actatttgcc agaagatgtg aaagtaactg acagaatgat gattactgct cttataacgg
     1021 aacatagtta ctgggggcaa attcatagaa attcaaaata taaatatcgt gaattgttaa
     1081 agcaagaaag tcaaaatgat gagttgacga atcatttgcg agaaacattt tcggagtcgg
     1141 cagatgattt gaaacga
//
LOCUS       DACDOA010000238         1146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDOA010000238 DACDOA010000000
VERSION     DACDOA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.246"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1061)
                     /locus_tag="IWI39_002886"
     CDS             complement(<1..1061)
                     /locus_tag="IWI39_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209901.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          303 a          255 c          182 g          406 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatact
//
LOCUS       DACDOA010000239         1126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDOA010000239 DACDOA010000000
VERSION     DACDOA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.247"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1126
                     /locus_tag="IWI39_002887"
     rRNA            <1..>1126
                     /locus_tag="IWI39_002887"
                     /product="23S ribosomal RNA"
BASE COUNT          289 a          244 c          348 g          245 t
ORIGIN      
        1 gcgactgttt atcaaaaaca caggtctctg caaaatcgta agatgaagta taggggctga
       61 cgcctgcccg gtgctggaag gttaagagga gtgcttagcg caagcgaagg tacgaattga
      121 agccccagta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
      181 gtaagttccg acccgcacga aaggcgtaac gatttgggca ctgtctcaac gagagactcg
      241 gtgaaatttt agtacctgtg aagatgcagg ttacccgcga caggacggaa agaccccatg
      301 gagctttact gtagtttgat attgagtgtc tgtaccgcat gtacaggata ggtaggagcc
      361 gtagaaatcg gaacgctagt ttcgatggag gcgctggtgg gatactaccc ctgcgttatg
      421 gccactctaa cccgcaccac taatcgtggt gggagacagt gtcagatggg cagtttgact
      481 ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag gttccctcag aatggttgga
      541 aatcattcga agagtgtaaa ggcagaaggg agcttgactg cgagaccaac aagtcgagca
      601 gggacgaaag tcgggcttag tgatccggtg gttccgcatg gaagggccat cgctcaacgg
      661 ataaaagcta ccctggggat aacaggctta tctcccccaa gagtccacat cgacggggag
      721 gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc caagggttgg
      781 gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag acagttcggt
      841 ccctatccgt cgcgggcgtt ggaaatttga gaggagctgt ccttagtacg agaggaccgg
      901 gatggactta ccgctggtgt accagttgtt ctgccaaggg cattgctggg tagctatgta
      961 gggaagggat aaacgctgaa agcatctaag tgtgaagccc acctcaagat gagatttccc
     1021 atttctttaa gaaagtaaga tccctgagag atgatcaggt agataggtca ggagtggaag
     1081 tacagtgatg tatggagcgg actgatacta atcgatcgag gactta
//
LOCUS       DACDOA010000240         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDOA010000240 DACDOA010000000
VERSION     DACDOA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.248"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(39..995)
                     /locus_tag="IWI39_002888"
     CDS             complement(39..995)
                     /locus_tag="IWI39_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209902.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DACDOA010000241         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDOA010000241 DACDOA010000000
VERSION     DACDOA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.249"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(218..766)
                     /gene="aac(6')"
                     /locus_tag="IWI39_002889"
     CDS             complement(218..766)
                     /gene="aac(6')"
                     /locus_tag="IWI39_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9209903.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          314 a          215 c          150 g          366 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac
       61 tcgaaactaa gcgacttttt cacaacctct ggtacacggt gttccaaaag tagcttcttc
      121 ggaaataagt tggaatttca caaaaattca agaagtaatt ttcgtaaatt ccttcttatt
      181 tcttgaagct gattacttct gtcacaacct ctttttatta ttgagaattt ggtcgaggaa
      241 taatcgtttt tgccatccaa atatccggtt tgtcccagcc atttgcattt ggtaagacac
      301 ctacgatttt ataacctaat ttttcataga attcatacgg atgttcacga aggttctgga
      361 tggaagccac tttatcaaat gtatgctcat acaggtccgt ttgacttaac gttgttccat
      421 ggtctaaatc atccgtacct aaataaatcg tgattcctcc tctggaagct acttcttttt
      481 ctaagtaatt gactaatcga gtacctattt ggttctttcg tcgggagctt tctacaacta
      541 atggatgcaa ttcccaacct gtgataccgt attgagggat tgcaccaata aatcctacta
      601 actcatcttg gtctaccgct gctaccgcga ttcgttctgg attcatcatt tcttctactt
      661 cttctgccga gctgtctcca tattcttccg gccaagtcag tctcagtaaa tcagaaagct
      721 gatctttcaa tactggatta ttacggtcaa attcactgat tatcatacgt cttccccctt
      781 gttcttgctt attctaacag tccttttgaa aaaacgaaat gttctcgctc aaaaatcttt
      841 agctaccaat cagtatctct attctttcat tatttatctt acttataaaa taaaaaatta
      901 tacagaaaca ctttattcaa aataggaaag atcaggaaag ccatcaattg gaggaaaaaa
      961 ctctgtttgt tcatcatgat atagattatc ttttgcttta aaaaaaacca attatgaatt
     1021 gacaaataaa acctgttttc atata
//
LOCUS       DACDOA010000242         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDOA010000242 DACDOA010000000
VERSION     DACDOA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.250"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..673
                     /locus_tag="IWI39_002890"
     CDS             134..673
                     /locus_tag="IWI39_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209904.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            670..1008
                     /locus_tag="IWI39_002891"
     CDS             670..1008
                     /locus_tag="IWI39_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP9209905.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          384 a          136 c          196 g          325 t
ORIGIN      
        1 gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaaaataac ggtgtacatt
       61 tttaaaagga gatgtataat cgagatttag aaagcgtttt cctcaaaatt taatgaatta
      121 ggaagtgatt actatgaaaa aattatttag cgcacttttt attctgttat ttggctttat
      181 tggtattggg acttctggaa atatagttaa agctagcacg atggttgaac aaacaagtga
      241 aatggcaaaa acttttagtc aattaacacc agaagaacaa agttacttca aatccaaagg
      301 atttagtgat aaagatactt tttacactca aaagatagag cctcaagatc cgaaaacacg
      361 tttttctgca aatgtattgc ggttaaccgc atctactaaa aaaattacta atacgcaggg
      421 gcggacagaa tatattataa caagtcccaa ttcggcgatg tatcgtattt ctacacgctt
      481 gagtttaggt agtgcaaaat catatactca aacagtaaat ctagctaatt cttatggtta
      541 tggtggagga atgtatttta catttactgg gaaaagacaa tattacagtg taaaattagc
      601 tatgcaagtt tataatacat ttggtgtagg gtcagtctct tgcactgcgg gaggattgac
      661 aataggaaaa tgataatttc ttactatgtc ttaaatagaa gtgatgatga aggactcttc
      721 gtttttcaat cattacatga ggatacgatg ctaaaagagt tttataatcg gattaagaat
      781 aaacagatag aaacaaattt atatttttta agtgactcta tcttgccttt tattagcaac
      841 cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat tattgtcatt
      901 gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag aagaattaga aaaattaact
      961 aacattggaa tgagtattca ggaggataat ggggtacatg gaacatagaa tgaatatgca
     1021 cagttgagaa cggaatttga c
//
LOCUS       DACDOA010000243         1039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDOA010000243 DACDOA010000000
VERSION     DACDOA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.251"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..816
                     /locus_tag="IWI39_002892"
     CDS             1..816
                     /locus_tag="IWI39_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9209906.1"
                     /translation="MYNDFSPKEQAIADYILENPSKVSHSPISNLASELGIADSTFFQ
                     FTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLNDTK
                     KLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQLM
                     EASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVFIS
                     ISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
BASE COUNT          352 a          184 c          181 g          322 t
ORIGIN      
        1 atgtacaacg acttcagccc aaaagaacag gctatcgccg attatatttt agaaaatcca
       61 agcaaagtaa gtcatagtcc tattagcaat ttagcaagtg aactgggcat tgctgactct
      121 actttttttc aatttacaaa aaaacttggt tataatggat ttaaagattt taaaatggcc
      181 atgttgatcc aagaaaatga tctatctgct atttctatcc acgaaaatat tcagaaaaca
      241 gatactgagc tcactatggc gcaaaaggtg ttcgagtcta gtatttcaac tttaaatgac
      301 acaaaaaagt tgttacaaca aaaggatcta aaaaaagcgg ctactattat caatgaatct
      361 aaacgtgtct attttttcgg tgttggcggt tcagagattg tagcaactga cgcttatcat
      421 aaatttttgc gttcccctat cgcaacttct cacagcacgg attaccatat tcaattgatg
      481 gaagcttctt tattgacgga aaaagactgt gctgttttga tttctcacac agggcaatca
      541 aaagaaacaa tccatattgc tgaaaccgta agaaaaactg gagcaaaaac aatcgttatt
      601 actagccaag ctaattcttc ccttgctaaa cttggcgatg tcgtttttat ttctatttct
      661 gaagaaacag aattccgatc tgaagcttta gcttcccgta tttctcaact aagcattttg
      721 gactctctat atgtgatttt gatgttttac aacgaaaaag aatcacgtcg ttctatcgct
      781 aaagttcgac atactatttc tgaaatcaaa gaatagcacc ataacagaaa aagcgaaaaa
      841 tccataaaac aaaaaaagcc actaccaaat atttggtagt ggcttttttg gttctgcaag
      901 ggtggtacag aacatcaata gtcggtgggt tagaacgtat gtcatcgcat ctttggagaa
      961 ggttgtattg gtattattcg agttacttgt tagatgcgat aacaaagtta gtatacagta
     1021 gtaaaaaatt tatcttttc
//
LOCUS       DACDOA010000244         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDOA010000244 DACDOA010000000
VERSION     DACDOA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.252"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWI39_002893"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWI39_002893"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDOA010000245         1029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDOA010000245 DACDOA010000000
VERSION     DACDOA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.253"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..987
                     /locus_tag="IWI39_002894"
     CDS             28..987
                     /locus_tag="IWI39_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9209907.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          360 a          172 c          190 g          307 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagaaaat gaaaaaacag agccagatta
     1021 gcattattt
//
LOCUS       DACDOA010000246         1007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDOA010000246 DACDOA010000000
VERSION     DACDOA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.254"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..642
                     /locus_tag="IWI39_002895"
     CDS             28..642
                     /locus_tag="IWI39_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209908.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(807..>1007)
                     /locus_tag="IWI39_002896"
     CDS             complement(807..>1007)
                     /locus_tag="IWI39_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209909.1"
                     /translation="LTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEA
                     KSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          336 a          183 c          176 g          312 t
ORIGIN      
        1 aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc
       61 gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc
      121 atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat
      181 tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat
      241 gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact
      301 agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat
      361 ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc
      421 caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta
      481 tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat
      541 cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc
      601 gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta
      661 ttatcttctc atctttaatt tcctacatat aattactatc tataaaatta agtcttactc
      721 agaacagata aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct
      781 gttaatataa tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct
      841 atattttcta caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc
      901 gacacattgg ttatactaat tcctatagct agttcttgag actctacttt tctctctagc
      961 tcctttgaca aagtgatttg agatttattt ggttccgttg gagtcaa
//
LOCUS       DACDOA010000247         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDOA010000247 DACDOA010000000
VERSION     DACDOA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.255"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1003
                     /locus_tag="IWI39_002897"
     CDS             <1..>1003
                     /locus_tag="IWI39_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9209910.1"
                     /translation="KKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPF
                     CFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLL
                     FDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVT
                     DMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQ
                     LKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLL
                     TAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLHT
                     "
BASE COUNT          301 a          210 c          190 g          302 t
ORIGIN      
        1 gcaagaagat ctctatgatt ctcctgaatc gtagcggtaa taaacggacc tatcttcggt
       61 tgaagaaaca gcgatacctc tgccgagcat gtaaaaaata ttttaccgct cggacgtatc
      121 tcgttacccc attctgcttt atttctaagc agattcatta caagatctta gaagaattga
      181 cagagcgtca atctataaaa gcaattggta aacattgcga tgtttctgtg acaactgttc
      241 agcggacatt gtccgccttg tcatcagata tccgtcagaa tcttgagtat ttacctcggt
      301 gtctattatt cgatgagttt cgttctcttt ctacatggca cggtaaattt tctttttctt
      361 gtatggacgg tgagaccggg aaactgtttg agatcttacc tagccgccgt aaaaaagatc
      421 tcgttgcttt tttcatgcgt ttttctctca aagcgaggtt gggtgtacat tacatcgtaa
      481 cggatatgaa cgctccttat ttctcactag ttaaagagtg ttttccaaat gcacaagtga
      541 tcgtcgatcg gtttcacatt gttcaacatc tgaaccgctt cttcgatgcg gttcgaaaac
      601 gtgtgatgaa aacacttgat caaaaagacc caatacaagc taaacagtat cgccaattga
      661 aatccttatt caaattactt ttaaaatcag aggatcgttt agattacaat acactaacga
      721 aaagacgcaa tttcaattgg agactattga ctgaaacaga agtagttgac cgccttctaa
      781 agatttctcc tgaacttaaa acagcttatc gttactacca agacctgctt actgcgtatc
      841 aggaaaaaga tccagatact tttttccagc tgcttcgagc aatgcctgga gaagttccat
      901 tggaactgca ccatatcaaa aaagcctttt tcaaatacga gaaagggatt cggctggcat
      961 tactgaagaa gtattcgaat gcacgactgg aaaatctcca tac
//
LOCUS       DACDOA010000248         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDOA010000248 DACDOA010000000
VERSION     DACDOA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.256"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            370..1001
                     /locus_tag="IWI39_002898"
                     /pseudo
     CDS             370..1001
                     /locus_tag="IWI39_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DACDOA010000249         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDOA010000249 DACDOA010000000
VERSION     DACDOA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.257"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IWI39_002899"
     CDS             complement(<1..434)
                     /locus_tag="IWI39_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9209911.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IWI39_002900"
     CDS             complement(451..855)
                     /locus_tag="IWI39_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9209912.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          304 a          172 c          163 g          361 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac
      901 aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg taaccctcat
      961 accgaacggc attcatcacc cacctataga ggatgggcga
//
LOCUS       DACDOA010000250          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDOA010000250 DACDOA010000000
VERSION     DACDOA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.258"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWI39_002901"
     CDS             281..973
                     /locus_tag="IWI39_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9209913.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          425 a          142 c          166 g          261 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acct
//
LOCUS       DACDOA010000251          983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDOA010000251 DACDOA010000000
VERSION     DACDOA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.259"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..258)
                     /locus_tag="IWI39_002902"
     CDS             complement(1..258)
                     /locus_tag="IWI39_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9209914.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            416..679
                     /locus_tag="IWI39_002903"
     CDS             416..679
                     /locus_tag="IWI39_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209915.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            703..891
                     /locus_tag="IWI39_002904"
     CDS             703..891
                     /locus_tag="IWI39_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209916.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          382 a          128 c          189 g          284 t
ORIGIN      
        1 tcaattctga ttcttatata aaaaatagat ccaaataata agaaaaatta ctgaaagcgt
       61 caattcgatg atgtgtgtta ttaatacaga cgttgtttcc caatgcctaa aagaaaaaat
      121 ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact tcttttttgt
      181 ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat aaaaaacaaa
      241 ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata caaaataaca
      301 ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct gtatcgattt
      361 tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga ttttcatgga
      421 tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga tttactatgt
      481 tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta ttttagcaaa
      541 gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc cagtagaaaa
      601 aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag catatgaacc
      661 tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc atatgaacaa
      721 cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag agaagaatta
      781 atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt gggaaaagcc
      841 tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta gtatgaagta
      901 ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga ttttacaaaa
      961 gtgaaagttt tacttatttg aat
//
LOCUS       DACDOA010000252          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDOA010000252 DACDOA010000000
VERSION     DACDOA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.261"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IWI39_002905"
     rRNA            2..>943
                     /locus_tag="IWI39_002905"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDOA010000253          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDOA010000253 DACDOA010000000
VERSION     DACDOA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.262"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            92..412
                     /locus_tag="IWI39_002906"
     CDS             92..412
                     /locus_tag="IWI39_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209917.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            430..861
                     /locus_tag="IWI39_002907"
     CDS             430..861
                     /locus_tag="IWI39_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9209918.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          334 a          132 c          159 g          302 t
ORIGIN      
        1 atagtatagc tcattactca gaattagtca aattatttct gcgtattttt ctaaactttt
       61 tttgtaaaca tttagaaagg ttgttttatc gatgaatacc tatgaaataa taaaagactt
      121 ggcgaagagg aaaaagatat ctatacggca gttagaaatt aattttggat attctaacgg
      181 ttatctagca aagtggaaaa ataatactcc taattcagat gaattaagtc ggattgctga
      241 ctatttcgat gtctctgtag actaccttct aggtagagaa gaaagagaaa cccctaaaca
      301 tgtggattta tcagaagacg atactgtttt ttcttttgat ggaaaagaaa tatctaagga
      361 gacaatgcgt aaagcgattg caattgctaa agctttagag gaaaatgaat agttggagtg
      421 atggtttgta tgtatttgaa attgaaagaa atgttgagcg aatacaattt gaaagtagtt
      481 tatatggaga tgaaagagcc tggtttctac tatccaaaac ccagaatcat ttttttgaat
      541 gaaaatctat acggtgaaac tgcagaagcc tttcatttat ctcatgaact tgcacatttc
      601 agcgcctctc attttgaatt ttcagttttg tacgatactt ctacaacttt tcactcaaaa
      661 tttgaaacag aagcagataa aatagctatt ctaattttat taaatatcta tattgagaac
      721 gaactgactg atgaatctca gtttaatctc gaaaaattta tggagtatta ttctattcag
      781 aataagctca gatacacttg ttatgctgtc tgccaatgct attttaagaa aaaatattct
      841 tacgcaaggc aatatgtatg aatacatcaa gaattattaa aaaaaccgat ttcagcaaaa
      901 cattaaaaaa gcccgtgctg caacacg
//
LOCUS       DACDOA010000254          922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDOA010000254 DACDOA010000000
VERSION     DACDOA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.263"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>922)
                     /locus_tag="IWI39_002908"
     CDS             complement(<1..>922)
                     /locus_tag="IWI39_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9209919.1"
                     /translation="FNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEH
                     RLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRG
                     AQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFL
                     ATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPT
                     LLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICI
                     YAFYVLNIFSLLKTFFRSKVNYAFLGIVLIF"
BASE COUNT          364 a          151 c          114 g          293 t
ORIGIN      
        1 aaaaattaaa acaataccaa gaaacgcata attcacttta cttctaaaaa atgttttgag
       61 taaactaaag atatttaaca catagaaagc atagatacat ataaaagcaa ttataccact
      121 tgatacaagt acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt
      181 cacatctgta gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa
      241 atcggtctct ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca
      301 aatcgtaaaa cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc
      361 tttttctgga agtggagttt tatctgattc atttgactct ataacttcta cttttctatc
      421 aatcgataca ggaattgttg ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata
      481 taccaataga actaataata tgcttaccat ccatttgatt tttacaggta gtacttctct
      541 aatgctcttt ctaaattttg aattggcaaa caacaaaaga aataatacta aaaaagaaca
      601 aattactacc tgggcacctc tagacgaaga aagtgtaata taaaatattt gtacgataaa
      661 attcatcata taaaaaacac gaatcgtttt tgttattttt gaaattaata cactaattaa
      721 acttaaaata atagaagtat atccaaacat agctccaaca tttggacttg tatagacacc
      781 aaataaccga tgttcaataa aaccttgtct tgcaagtaat cctggcgttc ctgttgggac
      841 atgataagaa atcaaaaaaa taaattgata gatagatatt agcgcagcta aaaaaattat
      901 gataatgaaa acattattaa aa
//
LOCUS       DACDOA010000255          906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDOA010000255 DACDOA010000000
VERSION     DACDOA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.264"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..720
                     /locus_tag="IWI39_002909"
     CDS             <1..720
                     /locus_tag="IWI39_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9209920.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DACDOA010000256          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDOA010000256 DACDOA010000000
VERSION     DACDOA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.265"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..843
                     /locus_tag="IWI39_002910"
     CDS             <1..843
                     /locus_tag="IWI39_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9209921.1"
                     /translation="QKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSIT
                     KIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKL
                     YNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQ
                     WMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENC
                     NIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKEN
                     QVTS"
BASE COUNT          332 a          155 c          143 g          272 t
ORIGIN      
        1 caaaaagaat tcacagagta tgaatatatt gactttttta ttgatccctc tgtagatatt
       61 ttgcgaccta atctgccaat ttgtcgcgaa caacgcatct tagtgaatat tttagctcga
      121 ctttctatca cgaaaatcga atcggaagaa acaagcttgg agcaatacaa acttttaaaa
      181 aaagcaaacg ttcctcatgt tctttattca agagaaatgg tggagtcctt ttgtactgta
      241 tttgatttgt ttatacctga aaaagaaaag attctttact cattggcatt cgcacgaaat
      301 aaactttata atggatttct taacttcaat caaccaaata cgccccttcc tactttcatg
      361 ctttatcaag aacatagcgt gttgaaagaa gtccaaaccg ccattgaaca attttatatg
      421 gatttcagag aacaaaatca acacttgtta ccagttattt tagaaaagga aaatgtgcaa
      481 tggatggttg aagacctagt acatttgtat gatcgctaca aaaaacagcc gcgaattgtg
      541 attggtgtga actatacgcg tgatttttat atcagtaaag atctgatcgt taagatagaa
      601 caaatttttt caaatgaatc gatcaaaata caaaatgatt ttatggagaa ctgcaacatc
      661 gttatcagcg actgcccttt gaaacaattg ccttctcata tcaaaaaaat ctatatttta
      721 aaaggaatca tcacaccaga agactggaaa aaagtgatta tgaggatctc agaatatatc
      781 tttgaattaa aagaagaagc actcatggat caatcgaaaa aagaaaatca agttacttcc
      841 tagaccaaat aagatacata aatccgaatg cttattaaca acctccggat ttatgtatca
      901 ag
//
LOCUS       DACDOA010000257          895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDOA010000257 DACDOA010000000
VERSION     DACDOA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.266"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..830
                     /locus_tag="IWI39_002911"
     CDS             <1..830
                     /locus_tag="IWI39_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9209922.1"
                     /translation="AMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGK
                     DNVSPFEFIPWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVI
                     ESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGM
                     GGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGK
                     YEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          323 a          117 c          157 g          298 t
ORIGIN      
        1 atgcaatgat tggtattgcc gctggtatag ctgactaccc tctttattac gatgcgacaa
       61 atgaaaaagg attgagtatg gctgggctaa atttttctgg gtatgctgat tataaagaaa
      121 tacaagaagg gaaagacaat gtatctcctt ttgaatttat tccttggatt ttaggacaat
      181 gctcaacagt aggagaagct aaaaaattgt taaaaaatat caatttagca aatataaatt
      241 atagtgacga acttccttta tcccctttac attggctatt agctgataaa gaaaaatcaa
      301 ttgtcattga aagtatgaaa gatggacttc atatatatga taaccctgtg ggcgttctta
      361 ccaataatcc ttcatttgac tatcaattat ttaatttaaa caattatcgt gtcttatcga
      421 gtgaaactcc taaaaataat ttttcaaatc aaataagttt gaatgcctat agccgcggta
      481 tgggagggat aggcttgcct ggagatttat cctcagtatc tcgttttgtt aaagcgactt
      541 ttacgaagct gaattctgta tctggagatt cagagtcaga aagtattagt caatttttcc
      601 atatcttagg ttcagtagaa caacaaaaag gtttgtgtga tgtaggtgat ggaaaatatg
      661 aatatacaat ttattcttct tgttgcaatg ttgacaaagg aatctattat tatcgaacat
      721 atgaagacag tcaaattact gcaattgata tgaataaaga agacttagat agtcataagt
      781 taattagtta tccaattata gaaaaacaac aaattaaata tataaattag ttaatgtgtt
      841 gtgattgatt atttaatata gatataatac aaaaaggcaa agatattttc aaaac
//
LOCUS       DACDOA010000258          873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDOA010000258 DACDOA010000000
VERSION     DACDOA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.267"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            124..363
                     /locus_tag="IWI39_002912"
     CDS             124..363
                     /locus_tag="IWI39_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209923.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          276 a          161 c          133 g          303 t
ORIGIN      
        1 cctctccata tatgtattac taagactaat agatactata aaagacttga tagttttgca
       61 ttgattttgc ctaatactga gcataggtgt atgctaaacg atactataag gagtgtgtac
      121 ataatgaaaa gaattctgtt tatcagttta tcaaatttat tgttacttac tgcttgtggg
      181 acttctgcat caaagaacat accttcagat acgcaaattt cgaatagtat cgattcaaca
      241 atttctacaa atgagaaaaa ttctccggta gaaacttccg atataaaagt ttctacctct
      301 gcaaaaaaca gggtaaagaa actgtcagca cgtctccttt atttcaaaca aactctgaat
      361 tagcttcttc atcttcttca gcaaataatg atgatagttt cgcgtttact cctaactaaa
      421 tgcaaccttt acaaatgctt tacgataaca agcctgagct aaatgatcct gatatcagtt
      481 ttgcattttt tgaaatgatt ggatcggatg atctttttaa aactactagt ttgtctatcc
      541 gaaaatagga cagttctggc agtataggtt tttatcgagt ttctccacca ggcctcgtta
      601 cagagacaga ttctctaggc gaaccttttg aataaatcaa aaatcatgaa caatcactgc
      661 caactaaata aagtgccttc aaaagcttgg tcttctcgct tttgaaggca ctttatttaa
      721 atctttatga aactaaagta tgttcatatt tgttgctata gttatataga tattctgttg
      781 tttccaactc ataaacgtga gtatattttg ccactacttt tccgcatttt tctaatattt
      841 cattgcgatc gacgtcatga tattcgtcta cgc
//
LOCUS       DACDOA010000259          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDOA010000259 DACDOA010000000
VERSION     DACDOA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.268"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWI39_002913"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWI39_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>865)
                     /locus_tag="IWI39_002914"
     CDS             complement(570..>865)
                     /locus_tag="IWI39_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9209924.1"
                     /translation="WKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQ
                     IKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          264 a          151 c          143 g          307 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatt
//
LOCUS       DACDOA010000260          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDOA010000260 DACDOA010000000
VERSION     DACDOA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.269"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..324
                     /locus_tag="IWI39_002915"
                     /pseudo
     CDS             1..324
                     /locus_tag="IWI39_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            358..>854
                     /locus_tag="IWI39_002916"
     CDS             358..>854
                     /locus_tag="IWI39_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP9209925.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQG"
BASE COUNT          308 a           97 c          162 g          287 t
ORIGIN      
        1 attgattcga caggtctatg tttattagat gaattcttgg cggatatcag tctatttata
       61 atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa gttcagaaaa
      121 tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag tagtttagaa
      181 gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt aaagaagttc
      241 tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat aacagcttct
      301 gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc tttcgatatg
      361 ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg taaaagtata
      421 gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca aatgtatgaa
      481 aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc tacagcttta
      541 gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt ttaccagatg
      601 gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca aaagaaaaat
      661 gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagttaagga tattttaaaa
      721 cggtatctcg ataatcaagt ggagattttg atttttgatt ttaacgcatt aaagaaaata
      781 gcaaaggaaa aagatactat gatttttaag aatacaattt gtgttctttc aaccactgaa
      841 ttttacattc aagg
//
LOCUS       DACDOA010000261          833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDOA010000261 DACDOA010000000
VERSION     DACDOA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.270"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..371
                     /locus_tag="IWI39_002917"
     CDS             <1..371
                     /locus_tag="IWI39_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9209926.1"
                     /translation="GISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNK
                     VTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSL
                     IISSFNILRRNPKELLIDNN"
     gene            501..>833
                     /locus_tag="IWI39_002918"
     CDS             501..>833
                     /locus_tag="IWI39_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9209927.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          287 a          124 c          159 g          263 t
ORIGIN      
        1 taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa
       61 tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata
      121 atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta
      181 gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata
      241 tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg
      301 tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg
      361 acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt
      421 gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa
      481 gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag
      541 ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa
      601 aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag
      661 ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca
      721 ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg
      781 cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac atc
//
LOCUS       DACDOA010000262          832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDOA010000262 DACDOA010000000
VERSION     DACDOA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.271"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IWI39_002919"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IWI39_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9209928.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          304 a          132 c          154 g          242 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DACDOA010000263          783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDOA010000263 DACDOA010000000
VERSION     DACDOA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.272"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..716
                     /locus_tag="IWI39_002920"
     CDS             24..716
                     /locus_tag="IWI39_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9209929.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          281 a          151 c          159 g          192 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 ctt
//
LOCUS       DACDOA010000264          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDOA010000264 DACDOA010000000
VERSION     DACDOA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.273"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(596..739)
                     /locus_tag="IWI39_002921"
     CDS             complement(596..739)
                     /locus_tag="IWI39_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209930.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            658..>764
                     /locus_tag="IWI39_002922"
     rRNA            658..>764
                     /locus_tag="IWI39_002922"
                     /product="16S ribosomal RNA"
BASE COUNT          314 a          124 c          134 g          192 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtgcctaat acatgcaagt
      721 cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aaga
//
LOCUS       DACDOA010000265          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDOA010000265 DACDOA010000000
VERSION     DACDOA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.274"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..441)
                     /locus_tag="IWI39_002923"
     CDS             complement(151..441)
                     /locus_tag="IWI39_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9209931.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          261 a          123 c          111 g          261 t
ORIGIN      
        1 tgcgagcagc aactagaata atttaaacat tattaaactg tctatattgt tactaataat
       61 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
      121 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
      181 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
      241 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
      301 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
      361 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
      421 aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
      481 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
      541 ttcatagaga taactctata gaatccgtaa aataccttat atatataacg ggaaaatata
      601 aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaagc gctcaacacc
      661 aacctcattt atcaaacgtt ggccataacc ccaaatctct tctgatagct gatcaaaggt
      721 tatatacttt agctcatatt gctggataat gttcat
//
LOCUS       DACDOA010000266          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDOA010000266 DACDOA010000000
VERSION     DACDOA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.275"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..257
                     /locus_tag="IWI39_002924"
     CDS             <1..257
                     /locus_tag="IWI39_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9209932.1"
                     /translation="YTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSL
                     ILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            270..>754
                     /locus_tag="IWI39_002925"
     CDS             270..>754
                     /locus_tag="IWI39_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9209933.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     S"
BASE COUNT          288 a           92 c          122 g          252 t
ORIGIN      
        1 gatacacaac cgtatttacg tattttttac tttttatttt tcattataat atcgctaaaa
       61 aaattacgaa taaacaatta tttgacacaa aaaaaatatg tatcataata atttttgtat
      121 caattttttc tttgatactt catattttaa tcaatcaaat gttgattcgt tacgttttga
      181 catcattatt gataattagt ttagttatat attttttaaa gattaagaat cgaattatag
      241 atgactataa ttcataggag gagatataag tgtttaaagg aaaaacaata ttagtaactg
      301 gcggaactgg ttccattgga agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag
      361 ctatcagaat ttatagtaga ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa
      421 atcacactaa tttgagattt ttaattggtg atatcagaga ctctcaaagg attgattatg
      481 catgtcgtga tgtggatatt atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg
      541 aatataatcc tatggaagct gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg
      601 cagcgatcaa aaataatgta aaacattttg taggtatcag tacagataaa gtagtgagtc
      661 ctactaacac catgggtgcg acaaagttat tgagtgaaaa attaataata gctgccaatc
      721 agtataaagg aaaaacagaa acaatatttt cttg
//
LOCUS       DACDOA010000267          744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDOA010000267 DACDOA010000000
VERSION     DACDOA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.276"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..>744
                     /locus_tag="IWI39_002926"
     CDS             63..>744
                     /locus_tag="IWI39_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP9209934.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          243 a          132 c          154 g          215 t
ORIGIN      
        1 caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac
       61 gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg
      121 cgatgcttac gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg
      181 cgcttgattt tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc
      241 caagtatcga tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc
      301 gttccatgcg acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggtttttag
      361 gctactcctt cgaggagaaa atcccccatt tctcaacttt tggaaagaat tatgttcgcc
      421 gatttagaga aacgacggtg tttgaggata tttttgccta tattttagaa caagcagtta
      481 aagcaggttt tgtaacagag gataatttgt atctggattc tacccacatc aaagctaacg
      541 ccaataaaca taagtttacg aaagaaatga cccatgatga agcgaaagcc tatcaggatg
      601 aattagaaga tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct tttactttgg
      661 acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat ccggaaagcg
      721 gctactacgt caaaggcgaa cgag
//
LOCUS       DACDOA010000268          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDOA010000268 DACDOA010000000
VERSION     DACDOA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.277"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..676
                     /locus_tag="IWI39_002927"
     CDS             <1..676
                     /locus_tag="IWI39_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9209935.1"
                     /translation="SENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAESLGAQE
                     GMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDS
                     IATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDSYYVNAE
                     QELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSESFPKTI
                     TKDWLE"
BASE COUNT          289 a          113 c          113 g          177 t
ORIGIN      
        1 ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga
       61 taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct
      121 tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat
      181 gaaaatcata ccagaactta ataataatat attagacact aatagacaca atatagacac
      241 tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa
      301 cgacagcatt gctacgttta tccctgaacg agtattaaat ttaataaaaa cattttcttc
      361 aagttacagt gaagctcaaa aaactgtcca gactattcat aatgcaaaga aaaaagccga
      421 aacagaaagt ggtatttcaa taatttttga agaactcgat agttattatg tcaatgcaga
      481 acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagttga
      541 aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc
      601 tgctaaagta tcaagtgaaa aacgtttgaa ttctgaaagc ttcccaaaaa ctattacgaa
      661 agactggtta gagtgaaatt tcactgacaa at
//
LOCUS       DACDOA010000269          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDOA010000269 DACDOA010000000
VERSION     DACDOA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.278"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            160..492
                     /locus_tag="IWI39_002928"
     CDS             160..492
                     /locus_tag="IWI39_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9209936.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          227 a           99 c          132 g          198 t
ORIGIN      
        1 ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg atgcgttccc
       61 tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa
      121 cttattaaaa atatttaaag taagtgggtg gattatttta tgtcatatac aggaattttg
      181 agtctcgaag atatttgtca ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag
      241 aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta agaaatatat aatccagaaa
      301 gacaaagtct cagaggaaat gatttattac gctggtaaac aaaagcaaat aattttaaaa
      361 gatcctatcc ctttaaaaga actatatcca acaatcaaac atgtttatga ccaaaacggt
      421 gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt
      481 ttaatcagct gaattgatca ttctcataac ttgtatttta tcgtttttcg cttgcttgaa
      541 aatattgtcg agacttctaa aagaacttag aattactaga aaaataagga gtagacttga
      601 tctatcaaca attcctttcg gggggtgacg gggtaggttt ttacgattgg taacgt
//
LOCUS       DACDOA010000270          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDOA010000270 DACDOA010000000
VERSION     DACDOA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.279"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>635
                     /locus_tag="IWI39_002929"
     CDS             <1..>635
                     /locus_tag="IWI39_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9209937.1"
                     /translation="PDKIKNRRNTDQLKQRDTVIIACVIWGIINGYTSQRATYRAVCY
                     VLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRN
                     RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSE
                     EANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          225 a          120 c          111 g          179 t
ORIGIN      
        1 accagataag attaaaaacc gaagaaatac tgatcaactg aagcaacgcg atacagtgat
       61 tattgcttgt gttatatggg gcataattaa tggctatact agccaaagag ccacgtatag
      121 agcggtttgt tacgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg
      181 cttaagttca aacttagctt acactatcaa gattattcga tactttttca tcaagaagct
      241 caccaaaggt gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt
      301 tagaaataga caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa
      361 aaaatcttat ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat
      421 tacttactca atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga
      481 agaagcgaat cttcccaata tcctagggga caaaggatat attagccata aaatccatga
      541 aaagcttgcc cttaagggca ttactatatc cgttccgcca cgtaaaaaca tggataaatc
      601 agaaaaacta gaccacagct tacttggaaa acaaa
//
LOCUS       DACDOA010000271          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDOA010000271 DACDOA010000000
VERSION     DACDOA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.280"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          235 a          101 c           94 g          200 t
ORIGIN      
        1 cttaaagaaa atgggttgca tcctggagat acaatttttt tagataaaga aaaaattcaa
       61 gcaacaattc aaaaaaatct taactgctga gattgatgta gcaatatctc aatcatcaac
      121 aaagtacgat cctgcctttc aaaagcttat tgataaatgt ttattcatta taaaaataaa
      181 agaaaaattt atgtcagctt ttttcatcaa agtggtaatg ataattcgtc tattaattgt
      241 attttttagg aaatatggta taattgtttt aacgaaaagg taattcatga tacaccaaaa
      301 accactagct ggcgggctag tggttttctt ttacgttcta ttcagattaa cgggtaggct
      361 tactgtcttt tttggcaata attttgcaat caagccattt acagatgtaa tgcgcggata
      421 cttctgctaa gattgcaaaa aacatatcga aaaggtaatt cataatacaa cctcctcccc
      481 ttgccataat aggccggggt agcagacagc ataaatatta taccataact gaaaatttca
      541 ttttagtaca tttcaatata ttgatgaacc agcacaataa aacatgccat gactaacaga
      601 aaaaaactta ttaaaccaga tataacttgt
//
LOCUS       DACDOA010000272          615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDOA010000272 DACDOA010000000
VERSION     DACDOA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.281"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..593
                     /locus_tag="IWI39_002930"
     CDS             <1..593
                     /locus_tag="IWI39_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9209938.1"
                     /translation="QMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTD
                     VTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILH
                     SDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIE
                     IFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          256 a           82 c          103 g          174 t
ORIGIN      
        1 cccaaatggg acttacctgt caaatccgaa taaagcgata taaatcttat aaaggaacag
       61 taggaaaaat tgccaagaat gtgttaaaac gaaatttttc agtagataca cccaataaaa
      121 aatgggtgac agacgtcact gaattcaaga taaaaggaag aaaaatctat ctatctccta
      181 tacttgattt atttaacgga gaaataatta gttatagtat atcaactagt ccaacatata
      241 aactgattga agaaatgctt caacaagcaa ttaaaaaaaa gggaactgag ggttcactga
      301 ttctacattc agatcaagga tggcagtatc agatgccaca atatcaaaaa aaattaaaag
      361 agaataacat tattcaaagc atgtctagaa aaggaaattg cttagataat tctgtaatag
      421 aaaatttttt tggtgtgctt aaatcagagt ttttttatcg agaaaaattc cgatcaattg
      481 agatatttca aagtaaatta aatgagtata ttaggtggta taataacaaa aggataaaac
      541 taaagttaaa tggactatct ccagtagaat atcggaaaca gtccattaaa tagttcaact
      601 tttggggttc agttc
//
LOCUS       DACDOA010000273          590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDOA010000273 DACDOA010000000
VERSION     DACDOA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.283"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(127..561)
                     /locus_tag="IWI39_002931"
     CDS             complement(127..561)
                     /locus_tag="IWI39_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9209939.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          175 a          118 c           84 g          213 t
ORIGIN      
        1 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
       61 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
      121 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
      181 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
      241 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
      301 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
      361 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
      421 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
      481 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
      541 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc
//
LOCUS       DACDOA010000274          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDOA010000274 DACDOA010000000
VERSION     DACDOA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.284"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..270)
                     /locus_tag="IWI39_002932"
                     /pseudo
     CDS             complement(1..270)
                     /locus_tag="IWI39_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          171 a          112 c          124 g          180 t
ORIGIN      
        1 atagctatcg atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat
       61 ctcttgtttt aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt
      121 ccgagacagg ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg
      181 atacgcccaa ccttgatctg agtgaaacgt acgacgatag ggacaatcgt tagtggtctg
      241 gatagcttcc tccaaacctt ccattaccgc acgtgtttct gaatggtggt agcgaccgat
      301 ttgaacggct agggcggaaa tacggtgcaa atgcacggtg gtttcatgca atgcttacag
      361 ggcgtatttt gtaagggtat gtaacggaga tgatcccgac ggtaatggat gtgagaggcc
      421 aagccgacgc tgacggtaga gcaaacaaac atagttgcac tctagcgatc gttatttatt
      481 tttaacggtc aaactagggt acaactatgc ttaaaacact tcgcctcaac ttctggtagc
      541 aaaagtgatt ttgaaagtca aaatattttt tatgagttat ttataat
//
LOCUS       DACDOA010000275          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDOA010000275 DACDOA010000000
VERSION     DACDOA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.285"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWI39_002933"
     CDS             168..362
                     /locus_tag="IWI39_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209940.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          227 a           67 c           87 g          156 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaag
//
LOCUS       DACDOA010000276          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDOA010000276 DACDOA010000000
VERSION     DACDOA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.286"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>534
                     /locus_tag="IWI39_002934"
     CDS             <1..>534
                     /locus_tag="IWI39_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9209941.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DACDOA010000277          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDOA010000277 DACDOA010000000
VERSION     DACDOA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.287"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..>529
                     /locus_tag="IWI39_002935"
     CDS             33..>529
                     /locus_tag="IWI39_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9209942.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACDOA010000278          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDOA010000278 DACDOA010000000
VERSION     DACDOA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.288"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>528
                     /locus_tag="IWI39_002936"
     CDS             <1..>528
                     /locus_tag="IWI39_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9209943.1"
                     /translation="FNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGL
                     VGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELAL
                     GNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTS
                     GLDYINMERIADLIK"
BASE COUNT          204 a           59 c          103 g          162 t
ORIGIN      
        1 tttttaatag ttcgattgaa gttccctata tcgaaaaggt aagtgcagat gtattaaaag
       61 ttgaagaaat tacttatgaa aatattctaa aagacataaa ttttgatgtc aaagttggag
      121 atgttattgg tttggtaggg aaaaacggtg tggggaaaac aaccttacta agaatactca
      181 tgaagattat tcgccctacc aaaggaaaga taatcgaaaa taaaaaaaat ttgtcatcgc
      241 cgtttttagt aatgcaagaa atggattacc aatttttcac agaatcagtg aggagcgagt
      301 tagccctggg gaatgaaata gttagtaaag atcagcaaga aaaaatttta aaaaaaatgg
      361 aactttataa aatgaaagat caattaccgt ttgatttatc cggtggagaa aaacaaagat
      421 tattggtttc tatagcttca ttgtcaaaaa ctaatttatt tctatttgat gaacctacaa
      481 gtgggttgga ttatattaat atggaaagga ttgctgacct tattaaag
//
LOCUS       DACDOA010000279          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDOA010000279 DACDOA010000000
VERSION     DACDOA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.289"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..>509
                     /locus_tag="IWI39_002937"
     CDS             287..>509
                     /locus_tag="IWI39_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9209944.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPIL"
BASE COUNT          191 a           58 c           99 g          161 t
ORIGIN      
        1 gagcgactcc tttggtaggg tgatgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaacaac
      121 acaaaatatt atagaagcct tttttctaaa ggatttcaag gtgtctgttt gcatttatta
      181 aaaaaaagaa aaagtattgg caacagaagg cgtcaccaac actagatatt atagagccat
      241 aatatgctgt ataatatcta gtgtataggc ttaagtggag gtatatatga aaatattaac
      301 tgttgaagat gataatatga taagagaggg aataagtgaa tatctttcag aatttggata
      361 tactgttatt caagctaaag atggaagaga agccttgtca aaatttaata gcgatataaa
      421 tttggttatc ttagacattc agataccttt tataaatggt ttagaagtgt tgaaagaaat
      481 tagaaagaaa agcaatttac caattctaa
//
LOCUS       DACDOA010000280          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDOA010000280 DACDOA010000000
VERSION     DACDOA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.291"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..154)
                     /locus_tag="IWI39_002938"
     CDS             complement(<1..154)
                     /locus_tag="IWI39_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9209945.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESS"
     gene            complement(173..328)
                     /locus_tag="IWI39_002939"
                     /pseudo
     CDS             complement(173..328)
                     /locus_tag="IWI39_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            404..>488
                     /locus_tag="IWI39_002940"
     CDS             404..>488
                     /locus_tag="IWI39_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9209946.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
BASE COUNT          165 a           97 c           57 g          169 t
ORIGIN      
        1 tagaactttc atataaagcc ttttctattt cattgcttga tttcattatt gttaaacaag
       61 aatacaagca agaaagaact atagttaata tgataaaaat tattaaagtt ctatttttct
      121 ttcttgtaat atatgctata gcatttttta tcactttcct aactactcct ttctattttc
      181 ttactccata actaattgca tccactggac aagcattttt acatcgtcca catctgatac
      241 actccagatg attgcaattc tcaactgaat ctttattcat ttggcaaact tttttacatt
      301 ttccacaatt aatacactta tccttatcct gaatcggcta cactagacta gacagaaaaa
      361 ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgccaa gacgtcaacg
      421 aagaacctat tcaaaagaat tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc
      481 ttgcgcag
//
LOCUS       DACDOA010000281          483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDOA010000281 DACDOA010000000
VERSION     DACDOA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.292"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(118..191)
                     /locus_tag="IWI39_002941"
     tRNA            complement(118..191)
                     /locus_tag="IWI39_002941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Ile,seq:cat)
BASE COUNT          174 a           82 c           90 g          137 t
ORIGIN      
        1 atccataaaa ggatacataa aaaagccact catttgagtg gcaatgaaga aaagctttag
       61 cttgtataat actcttcaaa aaattctaac acagaacgat tcaattggct acattaatgt
      121 accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct gaccaactga
      181 gctaagggta gtggttgttg ccacataaag ccataaacaa tcaaccagta gaatgcgtgg
      241 caacaaacct gttatcgcat atcttggagt gtgactattt atgagtgata gtgaagatat
      301 gcgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca
      361 acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga
      421 ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc
      481 tat
//
LOCUS       DACDOA010000282          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDOA010000282 DACDOA010000000
VERSION     DACDOA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.293"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(82..>457)
                     /locus_tag="IWI39_002942"
     CDS             complement(82..>457)
                     /locus_tag="IWI39_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9209947.1"
                     /translation="PIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGR
                     HITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAIT
                     KRYIGITEDEIGASLRGFKLGG"
BASE COUNT          136 a           96 c           49 g          176 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggc
//
LOCUS       DACDOA010000283          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDOA010000283 DACDOA010000000
VERSION     DACDOA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.294"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          146 a           72 c           74 g          160 t
ORIGIN      
        1 tacatctttt aaaagatttt gggctttctc tttataaggt cctaattgtt ctttatgttc
       61 ttcgataaat cgttttgcgc catcgaaatt gccgttctta atcatttcct tgactttttg
      121 aaataattct tcttgattca ttttgaacac atcctttgct atcaacatga ttttttacat
      181 ggacaaaacg agaaaaactg atttatccac acgtcaataa tatcaaataa cgatcatttt
      241 tactaaaaaa acactcgatt ctcgcttcta aagtaggaga aaaatctcca acttgggacg
      301 gatggaattt ctttaactga cccatatact gaatatataa caaggaggta gaaagatgaa
      361 agtgattaaa tatttgattg gactggcggc gattagtggt attttgctat ttggactgaa
      421 atattatact gaaggaagtt atggagaaat cc
//
LOCUS       DACDOA010000284          448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDOA010000284 DACDOA010000000
VERSION     DACDOA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.295"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(33..269)
                     /locus_tag="IWI39_002943"
                     /pseudo
     CDS             complement(33..269)
                     /locus_tag="IWI39_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a           76 c           77 g          150 t
ORIGIN      
        1 gaaggactga ccttctgtct cagatttttt tgttatttca cttgaatcaa ttgattgatc
       61 aaaaaaatcc tgattttcat gttctcaaat gatttaaatc cataagataa tcgtttcagt
      121 gtcttgatat ggggaatctt ggcctctatt ttgccattag aatacgggta aatcatagca
      181 ttgtaaatac cttcttcata cgttaaaagg ttttgaagtt tcgtgcgaag ttgtgcatca
      241 agtgtttcag gaagattttt taataacgcc gtttgtataa ttaagcgaga caaataaaaa
      301 agccatttat gttacactcc aattgtaata cccgctaaag catacaagga gagtgaacat
      361 aaatgactta tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc
      421 aagaaactcc ggttgctatc gttgcgaa
//
LOCUS       DACDOA010000285          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDOA010000285 DACDOA010000000
VERSION     DACDOA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.297"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          142 a           53 c           73 g          152 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatact tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acgatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg tcattagaaa
      181 atgatggtaa attatttgat ggtaacggtg atgtaacaac ggtaatgaaa ttattgcttt
      241 aacagtttag cgtgataaaa atctatacct tcttaagttg atttattaac ttgagaaggt
      301 atttttttct gaaaaatgat aatgaaaatc aaaatgttca tttctatcaa ttttgaagtc
      361 aaaaaaataa agtacagccc agaaatttgc tctgtacttt aaaaaactag ttaaatgaag
//
LOCUS       DACDOA010000286          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDOA010000286 DACDOA010000000
VERSION     DACDOA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.298"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..385
                     /locus_tag="IWI39_002944"
     CDS             188..385
                     /locus_tag="IWI39_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209948.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          164 a           56 c           95 g          105 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
//
LOCUS       DACDOA010000287          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDOA010000287 DACDOA010000000
VERSION     DACDOA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.denovo.300"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..218)
                     /locus_tag="IWI39_002945"
     CDS             complement(<1..218)
                     /locus_tag="IWI39_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9209949.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          123 a           85 c           50 g          150 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatga
//
LOCUS       DACDOA010000288         8014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.110, whole genome shotgun sequence.
ACCESSION   DACDOA010000288 DACDOA010000000
VERSION     DACDOA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.110"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            529..825
                     /locus_tag="IWI39_002946"
     CDS             529..825
                     /locus_tag="IWI39_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP9209950.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            826..1242
                     /locus_tag="IWI39_002947"
     CDS             826..1242
                     /locus_tag="IWI39_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9209951.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1262..2806
                     /locus_tag="IWI39_002948"
     CDS             1262..2806
                     /locus_tag="IWI39_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9209952.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2949..3173
                     /locus_tag="IWI39_002949"
     CDS             2949..3173
                     /locus_tag="IWI39_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9209953.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3188..4057
                     /locus_tag="IWI39_002950"
     CDS             3188..4057
                     /locus_tag="IWI39_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9209954.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            4038..4772
                     /locus_tag="IWI39_002951"
     CDS             4038..4772
                     /locus_tag="IWI39_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9209955.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4805..5713
                     /locus_tag="IWI39_002952"
     CDS             4805..5713
                     /locus_tag="IWI39_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9209956.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5710..6252
                     /gene="sat4"
                     /locus_tag="IWI39_002953"
     CDS             5710..6252
                     /gene="sat4"
                     /locus_tag="IWI39_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9209957.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            6345..7139
                     /locus_tag="IWI39_002954"
                     /note="aph(3')-IIIa"
     CDS             6345..7139
                     /locus_tag="IWI39_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9209958.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(7247..7435)
                     /locus_tag="IWI39_002955"
                     /pseudo
     CDS             complement(7247..7435)
                     /locus_tag="IWI39_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7415..7987
                     /locus_tag="IWI39_002956"
     CDS             7415..7987
                     /locus_tag="IWI39_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9209959.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         2779 a         1269 c         1789 g         2177 t
ORIGIN      
        1 aaaaagaacc ttttttcctg aataattcat agttttacta atagtctttc ctaacattga
       61 tttaatagtc tttaaagtat tctgcttcat cacccattag gtgatgaaaa aagaacccct
      121 ttctgattta gcggacaaat actttgacct aatctcagaa gatataaaag aaaaaataat
      181 aaaatatccg atttatactt tgtataaaga actaaagtag ttagcaaatc atttacagca
      241 aatcactatg ttccacatac cattttacat acttacctta tccacgacac atgttgagat
      301 agtatcgaag ataaagaaag ttgctccatg ttgtttttgc gagatgggga aatcaaagcc
      361 acattgacaa ccatgatgaa taagcttgct ttcagtcata agctaatcct agaaccactt
      421 ttcaagtcag ttagccaaat cgatgaagaa agcgaccgtg aaagaatgga cgctattgat
      481 aagctaatgg agcaactatt ggaagaacgc aataccctta ttgcactgat gtccaaaggt
      541 ttcctagaac ctgctctttt taatcaggaa cgaaatgttt tagatagtga gattaaaaat
      601 cttactaccg aaaaaacaaa cctggtaacg aactctgcga gtggggtttt gcgagcaaac
      661 gagataaagg acctcattaa ttatgtgtca gcagataatt ttaatggtga ctacacggaa
      721 gaactatttg aagaatttgt agtgaacatc attgtaaatt ccagggatga gctgacattc
      781 aatttgaaat gcggtctttc cctgaaagaa aaggtggtga gataaatggc atatattcca
      841 tatggataca aaattcaaga tggagtggtt actgtcgatg aaaaggcagc aggtcaagta
      901 aaggtattct ttgagaaata catatcagga ctatccctta cagtggctgg cgaacaggca
      961 ggtattgaaa agacacattc tgtgatgggg cgcattttga aaaacgtcaa ctaccttgga
     1021 aatgatacgt atccagcaat tattgataaa gagatatttg ataaagctga agaagttaga
     1081 gataaacgtg caaaggattt aggacgagtg gtagagcttg ccgctttcac ctctccccct
     1141 cccaaagaac gattcaaaat gaaaaaggca gataataaga tgccagtgga tccttttgaa
     1201 cgagcagaat acttatacag tctgatagaa agcgaggaat aaagtgacag agaaaaatat
     1261 aatggttatt cctgctcgta aaagagtagg aagtacagcc gcaaaagaaa agataaagaa
     1321 acttcgtgtt gctgcctatt gccgtgtttc tacagaaaca gaagaacaaa attctagcta
     1381 tgaggttcag gtctcacact atacagagtt tataaagaaa aataatgaat gggagtttgc
     1441 tggcatcttt gcagatgatg gtatctctgg tacgaacact aaaaagcgtg acgaatttaa
     1501 tcgcatgatt gcagagtgta tggacggtaa catcgacatg gttattacaa aatccatcag
     1561 ccgatttgcg cgtaacaccc tagactgcct tcaatacatt agacagctca aggataagaa
     1621 catatcagtt tattttgaga aagagaacat caacaccacg gatgccaaag gtgaggtttt
     1681 gctgactatt atggcatctt tggcacagca ggaaagccag agcctttcac aaaacgttaa
     1741 gcttgggcta cagtaccgat accaacaagg aaaggtgcag gtcaaccata agcgatttat
     1801 gggctacacg aaagatgaag atggaaattt aactattgtc cccgaagaag ctgagattat
     1861 caaacgcatc taccgagaat accttgaggg tcagagtcta gtgggcattg gtcgagccct
     1921 tgaaaaggat ggtattttaa cagcagcggg aaaaccaaga tggcgaccag aatcagttaa
     1981 gaagatactt caaaacgaaa aatacatcgg agatgccctt ctgcaaaaga ctgtcacagt
     2041 agattttctg accaagaaac gagtgaaaaa tgaagggcat gttccccaat attatgttga
     2101 aaatagccat gaagcgatta ttcctaaaga gttatttttg caggcgcaag aagaaattca
     2161 tcggagaagc aatatctaca caggagaagg caagaacaaa agaatttaca gtagcaagta
     2221 cgctttaagt gccatcacct tctgtggaga ttgtggcgat atttatagac gggtctattg
     2281 gaatatccac ggaagaaaag aatttgtctg gcgatgcgtg actagaatcg agcaaggtcc
     2341 tgaagtttgt aagaaccgaa ccgtaaaaga agatgaactt tatggtgcag taatgactgc
     2401 gattaataag ctacttgcag gtgggaacaa tatgataaag acactggaag agaatattca
     2461 tgctgtgatt ggcgaaacca cagaatacca aatttcagag attaacaact cactagagga
     2521 aaaacaaaaa gaactaatca agctggcgaa caagggtcaa gactatgacc acttagcaga
     2581 tgagattgat gagctgagag acaagcgaca gatcctttta gtagaagatg cctccctcag
     2641 tggcgagaac gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgcac
     2701 cttagaatat gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaagg
     2761 ccacttcgtc atagccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa
     2821 tagcccatga ctctacagta gagttgtggg ttttcttttg cttgctgatt ataataatat
     2881 tttaataact attgtgttta tcaaccaaat ggtttataat attcttagcg taaacttgga
     2941 ggtgctgtat gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg
     3001 tttccgaact tgctagacgt attggccaga ctccacagaa tttcaataaa aaattacaac
     3061 gagaaacagt aaccttggat gagttgaagg ccattgctga tgtgctaggt gtcaagtttg
     3121 tgcaggcatt tattttacca gatggagaag aaataaaaat atctaacgaa taaaggagga
     3181 tgtttatatg gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt
     3241 tatagaaggc atcgtattag ggggctcacg tgcaagaggc acccatacag aaaattctga
     3301 tatagatatc ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat
     3361 tgctacagag ttggatgatg agaatagaaa caaccttgtt gtacctcccg gagcgtgggg
     3421 tgattgggtt aatggcggtg gatggttagt tataaacggg tgtcatgttg acttgatatt
     3481 acgtgatata aaacgggtgg aacaaataat caaagatacg gagcaaggaa ttgttactgc
     3541 caattatcag accgggcatc cccatggtta tataagtgct atgtatcgtg gagaattagc
     3601 gattagtaaa atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga
     3661 aatttaccct actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt
     3721 ctctttaatg tttgtaaaag caaatgcggg ggcagaggat aaatattata ttgcaggcca
     3781 tgtttttcgt attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg
     3841 cattaacgaa aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata
     3901 tgccgaaaga gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga
     3961 catgaccgag aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt
     4021 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
     4081 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
     4141 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     4201 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     4261 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     4321 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     4381 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     4441 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     4501 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     4561 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     4621 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     4681 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     4741 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     4801 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     4861 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     4921 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     4981 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     5041 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     5101 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     5161 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     5221 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     5281 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     5341 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     5401 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     5461 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     5521 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     5581 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     5641 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     5701 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     5761 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     5821 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     5881 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttac
     5941 taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct
     6001 tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt
     6061 atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc
     6121 caggacaata accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc
     6181 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     6241 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     6301 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     6361 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     6421 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     6481 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     6541 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     6601 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     6661 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     6721 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     6781 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     6841 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     6901 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     6961 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     7021 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     7081 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     7141 acctagattt agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc
     7201 tgaagtacat ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta
     7261 gatagagttc tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt
     7321 ttataaccag ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat
     7381 actttttatg gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc
     7441 tattcttatt cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt
     7501 taatataagc ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc
     7561 aattggatct gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt
     7621 aatgtttaca gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca
     7681 tggagatgaa tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt
     7741 gaagcatggt gaaaaaaact atgcacagga gttggagaac agtattaacg tgtacagcac
     7801 atctggacag aaaaatacat tggcggataa cgttattgca gcaattcaga ctgcaatctg
     7861 caataaaagg gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac cggaaagcag
     7921 aatgatagag cctatatcac tggggtttta tgaacagaac tggtacttaa tcggttttgc
     7981 aggctaagaa atgaatatag gaattttcgt gtgg
//
LOCUS       DACDOA010000289         5541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.136, whole genome shotgun sequence.
ACCESSION   DACDOA010000289 DACDOA010000000
VERSION     DACDOA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.136"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..742
                     /locus_tag="IWI39_002957"
     CDS             <1..742
                     /locus_tag="IWI39_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9209960.1"
                     /translation="FLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDE
                     PAIDEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYK
                     QEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFE
                     SMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDA
                     ENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(892..1833)
                     /locus_tag="IWI39_002958"
     CDS             complement(892..1833)
                     /locus_tag="IWI39_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9209961.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1959..3080
                     /locus_tag="IWI39_002959"
     CDS             1959..3080
                     /locus_tag="IWI39_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9209962.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3338..4840
                     /locus_tag="IWI39_002960"
     CDS             3338..4840
                     /locus_tag="IWI39_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9209963.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4907..5434
                     /locus_tag="IWI39_002961"
     CDS             4907..5434
                     /locus_tag="IWI39_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9209964.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1799 a          987 c         1185 g         1570 t
ORIGIN      
        1 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
       61 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
      121 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
      181 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      241 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      301 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      361 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      421 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      481 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      541 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      601 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      661 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
      721 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
      781 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
      841 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
      901 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
      961 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     1021 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     1081 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1141 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1201 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1261 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1321 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1381 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1441 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1501 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1561 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1621 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1681 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     1741 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     1801 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     1861 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     1921 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     1981 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     2041 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     2101 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2161 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2221 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2281 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2341 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2401 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2461 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2521 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2581 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2641 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     2701 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     2761 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     2821 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     2881 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     2941 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     3001 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     3061 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3121 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3181 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3241 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3301 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3361 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3421 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3481 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3541 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3601 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3661 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     3721 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     3781 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     3841 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     3901 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     3961 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     4021 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     4081 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4141 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4201 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4261 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4321 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4381 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4441 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4501 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4561 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4621 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4681 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     4741 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     4801 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     4861 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     4921 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     4981 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     5041 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     5101 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5161 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5221 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5281 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5341 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5401 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     5461 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggtgtgcgc cctgcatggg
     5521 tgaaaacttg gtggtgaaag t
//
LOCUS       DACDOA010000290         4846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.144, whole genome shotgun sequence.
ACCESSION   DACDOA010000290 DACDOA010000000
VERSION     DACDOA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.144"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..442
                     /locus_tag="IWI39_002962"
     CDS             <1..442
                     /locus_tag="IWI39_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9209965.1"
                     /translation="LRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKM
                     PKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGVEA
                     DQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            796..2274
                     /gene="msr"
                     /locus_tag="IWI39_002963"
     CDS             796..2274
                     /gene="msr"
                     /locus_tag="IWI39_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9209966.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2321..2767
                     /locus_tag="IWI39_002964"
     CDS             2321..2767
                     /locus_tag="IWI39_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9209967.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2966..3481)
                     /locus_tag="IWI39_002965"
     CDS             complement(2966..3481)
                     /locus_tag="IWI39_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9209968.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3598..4800
                     /locus_tag="IWI39_002966"
     CDS             3598..4800
                     /locus_tag="IWI39_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9209969.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1466 a          810 c         1052 g         1518 t
ORIGIN      
        1 cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg caatggtcat
       61 gatttttggc gatatgcaat gttacgaata tggcgaagaa atctataacc aagcttacga
      121 atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca ttccttatta
      181 caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc
      241 tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa ttggtggagt
      301 cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg ttccagttat
      361 tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt tagcaccaga
      421 aaagatcact acgttcgaat aaaaggtttt cagttactat tcagtagcta ttgctttttt
      481 tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa
      541 ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta taaacaacaa
      601 tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga aagctatgac
      661 agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt gatgcagttt
      721 ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt tacatgaaat
      781 aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt
      841 ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg
      901 atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt
      961 tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc
     1021 tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg
     1081 gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt
     1141 cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga
     1201 aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt
     1261 agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga
     1321 gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa
     1381 aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg
     1441 atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag
     1501 caacaaatca gaccggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc
     1561 atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga aacaaccaag
     1621 ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc
     1681 tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat
     1741 ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt
     1801 aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa
     1861 gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt
     1921 cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac
     1981 ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg
     2041 acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa
     2101 ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat
     2161 ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa
     2221 gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc
     2281 tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaaaga
     2341 aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga gcgcgtataa
     2401 tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag tgcctggatt
     2461 cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt
     2521 taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt catgcttgat
     2581 ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac aaggaatacc
     2641 tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg ccgctttacc
     2701 gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa
     2761 tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt catagtctct
     2821 tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt cgttcggatc
     2881 gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct tcaatagata
     2941 tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt tctcttactt
     3001 gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc acaacataac
     3061 gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga tttttttgca
     3121 tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt gttttatcga
     3181 gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga gcgggagttt
     3241 cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg tagatctttt
     3301 tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct aacttggcaa
     3361 gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt
     3421 catacccaga acgtggtttg gataaaagca cgcataataa tatataggct aaagtattca
     3481 taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt tccacgtttt
     3541 acatgtgcat atttacatgt aaaataaatc atgtatgatg ctaatagaga gaaagggatg
     3601 gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac aaaggggaaa
     3661 agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt gggtctagta
     3721 gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga aacgaccctg
     3781 ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg tttcttatct
     3841 agtcggatcg gtttgaaaaa aacattgatg acaggattgt ttatcatcgt tgtattcgct
     3901 ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc cggctggggt
     3961 gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat tttagttggt
     4021 aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat ggctgttggc
     4081 cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt tggggtagca
     4141 tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac tgagaaacca
     4201 aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca
     4261 gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc gtatgcgcca
     4321 tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg ctggggtgtg
     4381 cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt tacgacttat
     4441 cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt aggagctgga
     4501 acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt ccaaggaatt
     4561 atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg aaacgttgct
     4621 tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt tattgcaggt
     4681 gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc cattgttctt
     4741 atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga agaagaataa
     4801 gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DACDOA010000291         4768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.146, whole genome shotgun sequence.
ACCESSION   DACDOA010000291 DACDOA010000000
VERSION     DACDOA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.146"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            279..776
                     /gene="dfrG"
                     /locus_tag="IWI39_002967"
     CDS             279..776
                     /gene="dfrG"
                     /locus_tag="IWI39_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9209970.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(848..2800)
                     /locus_tag="IWI39_002968"
     CDS             complement(848..2800)
                     /locus_tag="IWI39_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9209971.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2807..3103)
                     /locus_tag="IWI39_002969"
     CDS             complement(2807..3103)
                     /locus_tag="IWI39_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209972.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3445..3735
                     /locus_tag="IWI39_002970"
     CDS             3445..3735
                     /locus_tag="IWI39_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9209973.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1531 a          772 c          830 g         1635 t
ORIGIN      
        1 atactaagta gttcagtttt ggagtacaaa atgacaaaat gtacttcaaa atggactact
       61 tttttcattt taacttacat accaaattac atacaaaatg atttataaaa tgcaattgaa
      121 aatgataatt gaatgaaaat ttgtgatttt gtggtattat gataatataa cttatagaaa
      181 aaattgctta ttcaactaac gggcaggata atgagaaaag gttttttgat agaaataaga
      241 aattagatgg taaattagtt aaaaaggctg tgttataaat gaaagtttct ttgattgctg
      301 cgatggataa gaatagagtg attggcaaag agaatgacat tccttggagg attcccaagg
      361 actgggaata tgttaaaaat actacaaagg gacatccgat aatattaggt aggaagaacc
      421 ttgaatcaat cggaagagcc ttacctgaca gaagaaatat tattctgacg agagataagg
      481 ggtttacctt taatggttgt gaaattgttc attcaataga agatgttttt gagttatgta
      541 aaaacgaaga agaaattttt attttcggag gagaacagat ttataatttg tttttccctt
      601 atgttgagaa aatgtacatc acaaaaatac atcatgaatt cgaaggagat actttttttc
      661 cagaagtgaa ttatgaggaa tggaatgagg tatttgccca aaaagggata aagaatgata
      721 aaaatccgta taactactat tttcatgtat atgaaagaaa aaacttattg agttaacggg
      781 tgcgttaatt gaagaagaac agtaaagata aagcacaaga gcttgggatt actccaagct
      841 ctttttgtca tttgaaataa attatatctt tccaatcaaa ttgtttatct gtaatcccta
      901 atctttgagc aggtgtcaaa acctttttat ccgctgattt aaaagggcga caaaagttgt
      961 agtaagttct tagaattgta atcgcaaatt gagcatactt cggattgaag ttagcgtaaa
     1021 tatagctctt cttatcacca cgagctgttg ttaatggtct ttcaagagat gatatataac
     1081 gtcttatctg ttgaataaag ctattggtcg aatggtcatt aacatttaat agcatctttg
     1141 cgatttcgtt tggttccaga gcagataaat ccgttctaca atcaactgaa taaaatcctt
     1201 tatccttagt ggctaatgga tgtttgatag ggttatcggc atatttgcga tatctttctc
     1261 catctttact aatagcctct tcgtggaatt gatgacttgt tagtaattca gttagataat
     1321 gagatgccaa agtccgtaga aactttgttt tgaatcctct gatatctccc caatcgagca
     1381 gttctgcttt tgcttgtccg aattctttta agcattgttt tctgcttttc gtcttgttaa
     1441 cttggcaaat aaaatgatgt gcatcgctca accttaattc tttcgtgaat actctataaa
     1501 aggcgtttct aatagagaag tcatcatcac taatcattcg ccattcgtcg gcattaacca
     1561 tttgtttaat taaccaatag tgagcagttg tagtataagg agcatcaaca tttaaaccat
     1621 cgacaaatct actcctatta gtaattttcc ctaattcgtg aagataagca ttcctatttt
     1681 cgctatcgtt agcggatggt tcttgtggat aataagacca atctaaccta tcatttttac
     1741 gactaaaagt attgagatgg tcttctttga attttcttgt atcttcattt aattcatcca
     1801 ttgaaatatt ccaatcatag gcaacatcag aacggaacac atatcttgat aagacctcag
     1861 cagatacaac tatatacgtt tgcatattaa ggtcttcaaa ccccgtatat ttctttgaac
     1921 cttgtccttt cttacggacg ttatttaggt agtagtgcat cttgtccgta ttaagccaca
     1981 tctcgttaaa tttctttgtt tgaaggggtt gtgtctcata tcgttctaaa aactccaaac
     2041 aacgcctata cagccactcc agcttatgat agtaggttcc aactccaatt cctaaaatat
     2101 cacaagtacg actgactgaa acccttccaa caaccatctt agcgaacatt ggaagaatgg
     2161 tgttcttttg ttggtgataa gtagtggttt cttctctttt tggtagaaca ttagtgaact
     2221 tcttacacgc cttacattga tagcgttgag atttgcccct acttttcccc cttttgtaaa
     2281 attgcttcgg ctcattgaat ggggttgatt cttcttccga gcagccttct ttatgaaatt
     2341 gataatctgg ctcaacatct tggatgctat tgatttctat aaggcgttta atttcttcaa
     2401 caacagacca attagataaa ggggttactg tacagttttg cgatacacct ctgccaatag
     2461 gattgtcatt gcagtacata cctttatgac ctttatcttt ggaactacca gtcattttat
     2521 atcgacttgg tttccctttt acatctttga atttgtgctg ttctttccca aaattacaac
     2581 aaaacggatt gatgcagtaa ttatattgaa tgggatattc agaaccgttc catttaaaag
     2641 acgttggcaa gaacaacatt ttgttatacc ttgttgcaaa tcgttttgga gttaataaat
     2701 catattgttt agccctatct tttaattctt cgtttgaaat cggaatttta acatctacaa
     2761 tttcatcttc cttcgtggct aatcttttaa gcttggtcat tgtctatcaa cccactctat
     2821 ctttgatacc cattttttca acaatccgct tattacttcg cttgttggca atccattcaa
     2881 taaacttctt atcgtctaaa atgttcaata agaattcatc cactgcttcg ctttcagtac
     2941 gctctgcata ttcagcgtat tgcttaacta tcacccttac ttgctctgat atttcccaat
     3001 ccacagactt agcgttctta ttttttggct caagaaattg cataggaaaa tgcacccctt
     3061 tttgcttagt aaattgtaac cattttaaca caccaatttg cattcctaca ataattattt
     3121 cacgttcgca tttttatata cattttaact tacattttgt ggtgtaaatt aacaacaaac
     3181 gttgatttaa caataaaaaa cgagtatcaa attcagttgc aatttgatac tcatttaggt
     3241 ttacattttc actttttgta ctccaaaact gaactactta aatctatact gctgcctctt
     3301 ttttttagta catgtccttc atcttcgcag gaagtgccaa tatgatttta agaaaggtaa
     3361 tagacaaaaa gtcaaaaaga taaaacagag tttcttcttt acaaagggaa tgtatgttcg
     3421 tataatggta ttgaggtgaa ggttatggag aaaaaatatg gaacagtaga taagatccgt
     3481 actttgcaag ttagtgccag accgttgatc cgtttcagtt taaatggggt caattgtttg
     3541 attgcttccc atagtttgaa cttcctagct gaagtagacg aagggatgaa gttagtcgtt
     3601 acaggttatt ataataatag aaagcaattt gttgtgagat cctatcatgt gttagggaaa
     3661 cccaaaattg tggtagaata tgagaaaagt ctatacccaa gaaaaaaggt ccgacaagat
     3721 gaaagaataa actagaaaac aagcggctga gacaaaaatg tcctagccgc ttgttttgga
     3781 aagatggatg cagacgatgg gatttgaacc cacacgagca tacgctcaca agaacctcaa
     3841 tctatatata actacatgta tttaagtatt tgtgatgaat cactaaactt tccaaactca
     3901 tttgagggat attgcttaaa gattgaacag atcaattcga agccctatta gtattcgctc
     3961 aatgggggga tcagaggcca taaaaagaga ctagtcatta ctagtctcag gtagtggaat
     4021 tgtaggattt taaacgttcc tggagagtac cggtatgtaa ctcgaagagg tgattatcat
     4081 agtcgtaaaa gtaaattgaa gaagcctcgc ctttcacacg agaacgtcct ggttcgacag
     4141 ttaaacctaa catttggatt tttgagacaa acgaatctat atcttgttcg tcaattttaa
     4201 aagcaatatg attgtatgtc tttggcagtt tattcgacga attctgcatt actgcaatcc
     4261 ataggtcctt aactaagaaa aatttttctg gatataatga aaattttttt gttgactatc
     4321 gtagatttct tttgcatcaa ataaggtatt taacaattaa gtatttaaaa gataactttt
     4381 aagttatctt taagtaatgc ttaaagctat gttatactct attaggaaat ataagaagga
     4441 gtataaaaat gaacaaacat aatagaaaaa taacgaataa caaagaactg atcttgttac
     4501 taatagggat ttggggtatc tttttactct ttaatatgta tatgccgact atgagagcgg
     4561 atgacgtagt ttatgcaagt agattagatg aacttggcta tttgggtgct tcgatagaac
     4621 attataagac atggagttca agaattatta tagaactatt cttaatgttc ttttctaaac
     4681 actttatgct atggaaatta ttaaattcta cgattatgtt aggaattgta gtattacttt
     4741 gcaagtatgt tgagagtata aaatattt
//
LOCUS       DACDOA010000292         4608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.152, whole genome shotgun sequence.
ACCESSION   DACDOA010000292 DACDOA010000000
VERSION     DACDOA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.152"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..502
                     /gene="amaP"
                     /locus_tag="IWI39_002971"
     CDS             <1..502
                     /gene="amaP"
                     /locus_tag="IWI39_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9209974.1"
                     /translation="IALINAPYIMPEQLERFRFFTLTNYYMQQYIFWAAVVFAVLAII
                     ILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVRSHLHEEEFIHSPKVRIRSTKNR
                     IHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGTKETVKVAVTYSGYEEQEDTRDY
                     KHSRVE"
     gene            515..703
                     /locus_tag="IWI39_002972"
     CDS             515..703
                     /locus_tag="IWI39_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9209975.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            716..1273
                     /locus_tag="IWI39_002973"
     CDS             716..1273
                     /locus_tag="IWI39_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9209976.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1305..1547
                     /locus_tag="IWI39_002974"
     CDS             1305..1547
                     /locus_tag="IWI39_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9209977.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            1888..3279
                     /gene="amt"
                     /locus_tag="IWI39_002975"
     CDS             1888..3279
                     /gene="amt"
                     /locus_tag="IWI39_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9209978.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            3674..4147
                     /gene="dfrF"
                     /locus_tag="IWI39_002976"
     CDS             3674..4147
                     /gene="dfrF"
                     /locus_tag="IWI39_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP9209979.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4274..>4608
                     /locus_tag="IWI39_002977"
     CDS             4274..>4608
                     /locus_tag="IWI39_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9209980.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTG"
BASE COUNT         1546 a          705 c          982 g         1375 t
ORIGIN      
        1 gattgcgttg atcaatgcgc cgtatattat gcctgaacaa ttggaacgat ttcgcttctt
       61 tactttaacg aattactaca tgcaacaata tattttctgg gcagctgtag tatttgccgt
      121 tcttgctatt atcatcttgc tagtcgttct tttctatcca aagtcaagag gaactttcgt
      181 tatgaaacgg gaagatggca aactaacaat cgacaaaaaa gcgatcgaag ggttggtacg
      241 ttcccattta cacgaagaag agttcattca ttcacctaaa gtacggatac gttcaacgaa
      301 aaatcgaatc catatccatg tcaacggaga cttgaaacga acttcttcat tagtagggaa
      361 aacaggtgta ttgatgcaag atatcgaaga agaagtaact aaagtactcg gaacaaaaga
      421 aacggtcaaa gtggcagtca catacagcgg ttatgaagaa caagaagata caagagacta
      481 caagcactct cgagtggaat aggagggcca aataatgaaa gaactatttt cagcatacaa
      541 gctccctatt atctttgggg gtcttggact actgctagct gttttatggt taacgattgg
      601 atttttcaag acattgttga tcataatcat gacagcaatt ggcgcaggaa tcggttttta
      661 tttgaaacag tcaggaatag ttgatcaatt ttttaaatca taggaggaaa caaagatgga
      721 aaacaaaaac atgaatacaa caaatggatc agttttggca aatgaaaaag gagaaaaccg
      781 catgatgaat ggtgaattga cttatgaaga caaagtcatc caaaaaatca tcggttatgc
      841 attgaaagat gtagatggtc tattgacatt agatggcgga tttttctcaa atatggctga
      901 aaaattagtc aacacagata acgtaacagc aggtatcaat acagaagtag gtaagaaaca
      961 agtagctgtg gatctagatg tgatcgtaga atacggtaaa gacatcgaag atatttacaa
     1021 ccaaatcaaa gaattgatca gtactgaagt aaacaatatg acacatttag atgttatcga
     1081 agtgaatgtg aatgttgcag acatcaaaac acaagaagaa taccaaaaag atcaagaaac
     1141 agtacaagac aaagtaacag aagcagcaaa atccacagga caatttgctt cacgccaaac
     1201 agataaagca aaatctgctg taggcaaagg tgcacaaaaa gtcaaagaaa ataacgaacc
     1261 acgtgtacaa taagaagtaa gaagtcagtt aggaggaatt taaaatggga tttatttggg
     1321 cattaatcgt cggcggtatc attggagcaa ttgcaggagc aatcacgaat aaaggatcat
     1381 caatggggat catcgcaaac atcattgctg gtttagtagg ttcagcaatt ggacaagctt
     1441 tactaggaac ttggggacca acaattgcag gaatggctat cattccatca ttgatcggtg
     1501 caatcatttt agtattagtc gtttcattct ttgcacgtaa attataagca aattgaagaa
     1561 aaacttatgt tgaaatgaag aacaaatttg agaaccgtag cgacactcgc tgcggttctt
     1621 ttttatatat tttatatgaa gtattcttta ttccttatga aaaaatatct ttaaaaataa
     1681 aaaaagacga aaataaacgg ttttctataa aaagaagacc gtttattttt tgtctttatt
     1741 tgtgtgaaaa acgaaaaaga gcacaacaca tcaagctttt attaattgtc tgtcttttat
     1801 aaatgcaagt attaattcaa aaaaacagaa gaaaaaaaca actacacctt cttttatact
     1861 cgtaatcgta gaaaggaggc gaaaaaaatg aatacattac ctatcaatac aggagataca
     1921 gcattcatga ttttatgtac agcaatggtt tgcctgatga ctcctggtct agcttttttt
     1981 tacggcggat tagcacggaa gaataatata ctaacgatca tgtctcaatc ttttctctcc
     2041 gttgggataa caacgattat gtgggtattt ggtggattcg gattggcctt tggacgggat
     2101 atcggcggtg ttattggaaa cccggcagat ttctttttga tgagacatgt tacaagcttc
     2161 cccaacatgt ttcatggtgc aacgattcca tttttaatgt tctttcttta ccaactgatg
     2221 tttatcgtga ttaccgttcc tttgatgaca ggagcttttg cagggagatt gaatttaaaa
     2281 ggctatatta tacttgttat tttttggaat ctgctgattt atttccctgt ttgccactgg
     2341 atatggggtg gcggattttt ggatcagatg ggatttagag atttcgctgg tggagcagtc
     2401 atccacacga cagctggatt cggctcttta gcatgtatcc tcacacttgg tcaaagaaag
     2461 attcctttag gagaaagaag tcagcatagc agtcttatgg cagctgctat cggtacagga
     2521 ctgctttggt ttggctggtt cggttttaat tccggtggtg cacttcgagc agatgaacaa
     2581 gcagtcaatg catttggttc aacattcatc gctcttgcat ttgcgatgat tacttggctg
     2641 atcattgcaa aagtaaaagg aaatggcttt gattttgtcg acgtactaac aggatccgtt
     2701 gctggattgg cagccattac gccttgtgcc ggttatgtag aagcaaaaag tgccatgctg
     2761 attgggatca ttgcgggtat cgtctgtcat gctgctgttg attttcgtaa aaagaaacaa
     2821 tgggatgatg cacttgatgt gtggggtgtt catggcatgg gcggattcac aggtacgatt
     2881 ctgataggga tctttgcatc taacagccat ttactaacga atccaagcag ctggtacttc
     2941 ttagggattc aagtgattgg tgtcatcatt actgccgtgt atgcctatgt gctgacaaca
     3001 gtgattttaa aatcggcgat gcactttaca acgatcacta ctacaaaaga agaacaagaa
     3061 gaaggattgg actttttctt ctttggcttt ttggctatga ccatctcaat ggtcgtttca
     3121 ttatatgaat atcccacatt cgcgacctca ggcttcagct tagtattttt cttgttatta
     3181 ggcggtttgc tatggtttat ccctgtggca ttgtgcgcag cagagatggc aacggtgaaa
     3241 ggatgggaaa aaggtgtttc ccaaacaaaa agatattaag aaacagccca tgtttacctg
     3301 cttgcaaaca aaagaatatt gctactagac taagagatac tacataagtg tgttggtatc
     3361 tcttttttgt tgccgtgaaa cagcagacta tttcatttct gcttgattac cttaaaactt
     3421 attagtctat ggtttgtcaa gggcttgttg cgtgagaaat gctgaatatc accctttaca
     3481 aaacataggc tgataagtaa cttttcaaaa gcagaaaaga aatggcaatg cttataacaa
     3541 tatgatataa tgcagatgaa gacaaaaaag gaatttgtcg agctggttag ttcgagataa
     3601 ttttgagttt gttaagatgt ggtgcacttg atggaatcac aggtacgaca tcatattatg
     3661 gaggatgaaa tagatgatag gtttgattgt tgcgaggtca aagaataatg ttataggcaa
     3721 gaatggtaat ataccatgga aaataaaggg agaacaaaag caatttagag agttaacaac
     3781 gggtaatgtg gttattatgg ggcgaaagtc ttatgaagaa atcggtcatc cgttgcctaa
     3841 tagaatgaat attgttgttt ccaccacaac agagtatcaa ggagataatt tagtttcagt
     3901 taaatcatta gaagatgcat tattattggc taaaggacga gatgtataca tatctggtgg
     3961 atatggacta tttaaggaag ctttgcaaat agtagataaa atgtatatca cagaagtaga
     4021 tttaaatatt gaagatggag atacattctt tccagaattt gatatcaatg attttgaagt
     4081 tttgataggg gaaacacttg gtgaggaagt gaaatatacg agaacatttt atgtaaggaa
     4141 aaaatgaatt gagtaaattt tggatttagg gtgttttcat aaatatattg ctttttatgc
     4201 atatataaat tgttgtaaga caaattccag tttgtatggg gcgataaaat cagaatttga
     4261 gagaggattg aatatgaata aggaatggtc tgaacttaat aaaacaatgc aggcacaaat
     4321 aaaaaagaaa gatacttata agaggggtat tgatacttta cttactttac gaagtcagtt
     4381 aatacaaacc ttagtatctt tcaaagaaga attatgcaga gaagatttta actcaatccc
     4441 cttcataaac gctgatggtt atcatagtaa gacgattgct tattctattt ggcatatttt
     4501 ccgcattgaa gatatagttg tgcatacagt gataaatgaa gatgaacaag tattttttgc
     4561 aggcaattat caagagcgta tcaattcacc tatcattaca acaggaaa
//
LOCUS       DACDOA010000293         4340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.156, whole genome shotgun sequence.
ACCESSION   DACDOA010000293 DACDOA010000000
VERSION     DACDOA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.156"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..458
                     /locus_tag="IWI39_002978"
     CDS             <1..458
                     /locus_tag="IWI39_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9209981.1"
                     /translation="HPKMFWQRRYADRQTFISDEDIQEIRETYQKNTQLIIESLDNLT
                     DIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKKMLRGYYLERKIIDEYE
                     VAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFRAKSKKTSPIK"
     gene            618..2504
                     /locus_tag="IWI39_002979"
     CDS             618..2504
                     /locus_tag="IWI39_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9209982.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2516..3463
                     /locus_tag="IWI39_002980"
     CDS             2516..3463
                     /locus_tag="IWI39_002980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9209983.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3483..4010
                     /locus_tag="IWI39_002981"
     CDS             3483..4010
                     /locus_tag="IWI39_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9209984.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4073..>4340)
                     /locus_tag="IWI39_002982"
     CDS             complement(4073..>4340)
                     /locus_tag="IWI39_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9209985.1"
                     /translation="LTLHKRERVSKVSTIIYIFMGWLCVVAAVPLYHSLGMTGVILMV
                     AGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1546 a          739 c          922 g         1133 t
ORIGIN      
        1 tccatccgaa aatgttttgg caaagaagat atgctgatcg tcagacattt attagtgatg
       61 aagatatcca agaaatcaga gagacatatc aaaaaaatac tcagttgatt attgaaagtt
      121 tagataactt gactgatatt tatgatgaaa cgatggttca attttttatc cagcaacgca
      181 aacttgaagg aataaaaatg aactcgggga atttgatttc ttcgtggatg atccagcaag
      241 atagtctgtt tacgaaaaaa atgctaagag gttattatct tgaaagaaaa atcattgatg
      301 aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa cagctatcgc aggaatatca
      361 atttgttaga atcttatacg atgaacaaac catctgataa cttttctttc cgatttgcgt
      421 ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat tttcaagttt
      481 tataaaaaac aaaacatatc agaacaggat cgaatcggtc ctgttctttt tttgagaaca
      541 caaaaagaac aaataccaaa aaaattttcc tgaagttcac tatttttatg atacaatatc
      601 caagataagg agggaacatg ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg
      661 gtgaaaaaga tttgtttgac cagatttctt ttttggtcca cgaaaaagac cgtatcgggt
      721 tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata
      781 gcagcgacgg tgatcggcaa acagtgtttt atccaactga ttaccgtatt ggttatcttt
      841 ctcaagcagc tgaattttcc gatgaattaa ctgttctcca agcagtcttt caaggaaaca
      901 gccccttgat ccaaactgtt cgagcatatg aggaagcatt gatcgaacta ggggaaaacg
      961 gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg aataaagaag
     1021 atgcttggac tacagatacc aacgcaaaga ttattttgca aaaattagga atcagtgaat
     1081 tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag
     1141 tgttgattga tgaacccgat ttactattac tagacgaacc aacaaaccat ttagattatg
     1201 aagcgatcga gtggttggaa aactatttga aacaataccg tggcgcacta ttgatggtta
     1261 cccacgatcg ttattttcta gatagagtag caaaccgaat cttcgaactt tcatttggaa
     1321 aattatacga gtataaagga aattatgaag cttacgtctt agaaaaagca gaacgtgatc
     1381 gtgtagaagt agaacaagaa gaaaagcgca agcggcttta taaacaggaa ttagcttgga
     1441 tgagggctgg cgtacaggca cgtggcacca agcagcaagc acggatcaac cgtttcgaag
     1501 atctaaagga gaacttgtac caagtaaacc aagaagatga tttagagctg aacctagcga
     1561 cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt ataaatgacc
     1621 aaacactgct agaacattta gatttattga ttcaatctcg ggaaagacta ggtattactg
     1681 gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct agctggcaga attcccctgg
     1741 atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc gtattataca caagaaaatg
     1801 aagaaatggc accagataaa cggatgattg cttacttaca agaagctgct gaagaagcaa
     1861 aaacagcaga tggcagtcaa atcggagtag cagaattact tgaacgattt ttatttccaa
     1921 gatttatgca tgggacactg ataggcaaac tttcaggcgg agaaaaacgc cggttatttt
     1981 tgttgaaact gctgattcag caacctaatg ttttattatt ggatgaaccg accaatgatt
     2041 tggacatagc aacattgacg attttggaag attattttag aagttttcca ggtgctgtga
     2101 tcactgtttc ccatgaccgt tatttcttgg acaaggtagc agataagtta ttagtattcc
     2161 aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc cagttattta ctgaagcaaa
     2221 aagagaccca gcagcctgct gaaaaagcga aaccaaaaat acagagcaag gaacctgctg
     2281 aaaagaagaa attatcttac atggaacaaa aagaatggga aacgattgaa gatgagattg
     2341 cagaacttga agaaaaaatc tcattgctcc aagaagagat gaatcatcaa ggagataatt
     2401 tcactcgatt acaagaattg caaaacgacg tatctgaaac agaagcacaa cttgaagaaa
     2461 aaatggcacg gtgggaatac ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga
     2521 acaagcgtat cttgacttag gaagaaaatt gttggaagaa gggcatcgta aagaagacag
     2581 aacaggcaca ggcacattaa gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg
     2641 gtttcctttg ttaacaacga aacgagtacc ttttggattg atcaaaagcg aattgctttg
     2701 gtttttaaaa ggagatacga atatccgcta tctattacaa aataaaaacc atatttggga
     2761 tgaatgggca tttgaacgct acatcaaaag tccagactat aatggaccag atatgactga
     2821 ttttggccat agatgtttga cagatcctga attcaatgaa gaatataaaa agcagagcaa
     2881 gcttttttgc gagaagatcc taaccgatga ttcctttgct gaaacatacg gcgatcttgg
     2941 acacatctac ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca
     3001 aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat tctagaagat tgatcgtttc
     3061 tgcatggaat ccggaagatg ttcctagcat ggctttgcct ccttgtcata cgatgtttca
     3121 attttatgtt caagagggac ggctaagctg ccaattgtat caacgcagtg gggatgtgtt
     3181 tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa ttgcacatga
     3241 aacaggttta gcggttggcg aatttgttca cacattagga gatgctcatc tttatatcaa
     3301 tcatatcgaa caaatgaaag aacaattgag ccgagaaata cgcgcatttc cgactttagt
     3361 tttgaatcag gaaaagcagt cagtatttga ttttgagatg gaagatatcc aattagaagg
     3421 atatgaccca catccggcga tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga
     3481 aaatgttagc tgcaatttgg gcacaggatg aaaacggatt gattggaaaa gaagaccgtc
     3541 ttccttggcg tttgccaaat gatctgaaat tcttcaaaca aatgacagaa gcaaatacac
     3601 tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga
     3661 cgattgtttt gacgagagat tcttcctata aagcagaagg cgtgcatgtc atgcatagtg
     3721 tggaagacgt gcttgctctg gaaaaagaaa cagacggtat ctttttcatc gctggagggt
     3781 cagctgttta tcaagaattt ctgccatttt gtacgatatt ataccgtaca gtcatccatc
     3841 atgtattcga tggagatgct tattttccac ctgttgactg gtctgattgg tcactgatca
     3901 atactagtca aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc
     3961 agcgtaatga gaatcctgta aaaagcggga caggcgaatt gacgatataa aaaagctatg
     4021 gatagactag ctgttattta gagctgtccc atccatagct ttattttttt tattaagtaa
     4081 agaataaaac agagaagtac ataaaacctg cacctaacat aacaaacaaa tgccaaacaa
     4141 catgcatata gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc
     4201 cgcctgctac catcaaaata acaccagtca ttccaagaga gtgataaaga ggaacagcag
     4261 ccacgacaca gagccacccc ataaaaatat agatgatcgt agatactttt gatactctct
     4321 cccgtttatg caacgtcaac
//
LOCUS       DACDOA010000294         1208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.241, whole genome shotgun sequence.
ACCESSION   DACDOA010000294 DACDOA010000000
VERSION     DACDOA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.241"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            112..195
                     /locus_tag="IWI39_002983"
     CDS             112..195
                     /locus_tag="IWI39_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9209986.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            320..1057
                     /gene="erm(B)"
                     /locus_tag="IWI39_002984"
     CDS             320..1057
                     /gene="erm(B)"
                     /locus_tag="IWI39_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9209987.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          463 a          194 c          183 g          368 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gtaatgtaga taaaacatct accgttttga aacagactaa aaacagtgat
      181 tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt
      241 aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttattt atagatgtaa
      301 tcacttcagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
      361 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
      421 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
      481 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
      541 cactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
      601 gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt
      661 ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
      721 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
      781 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
      841 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
      901 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
      961 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
     1021 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
     1081 ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata gggcacctct
     1141 aataactacc aattaattca cctcaaaatg aaattagaaa aaaacagcga aatttttaaa
     1201 tctatttc
//
LOCUS       DACDOA010000295          944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.260, whole genome shotgun sequence.
ACCESSION   DACDOA010000295 DACDOA010000000
VERSION     DACDOA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.260"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            331..879
                     /gene="aac(6')"
                     /locus_tag="IWI39_002985"
     CDS             331..879
                     /gene="aac(6')"
                     /locus_tag="IWI39_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9209988.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          336 a          135 c          190 g          283 t
ORIGIN      
        1 attccctttg cttttttgta attatctaaa aattctaaaa gttaagatta ttatatgaaa
       61 acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca
      121 tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga
      181 ataaagtgtt tctgtataat tttttatttt ataagtaaga taaataatga aagaatagag
      241 atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt
      301 tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat
      361 ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga
      421 gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg
      481 gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt
      541 tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga
      601 ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt
      661 acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt
      721 gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta
      781 ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg
      841 atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa
      901 gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DACDOA010000296          600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E43003_2015
            SAMN12639490-rid9884173.guided.282, whole genome shotgun sequence.
ACCESSION   DACDOA010000296 DACDOA010000000
VERSION     DACDOA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639490
            Sequence Read Archive: SRR10030892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742197.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742197.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:05:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 71
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 30 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 9 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639490-rid9884173.guided.282"
                     /isolate="E43003_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>600
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI39_002986"
     CDS             2..>600
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI39_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9209989.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDN"
BASE COUNT          248 a           49 c          108 g          195 t
ORIGIN      
        1 aatgaatata gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt
       61 gatgttaaaa tggttaactg atgaaagagt attagaattt tatggtggta gagataaaaa
      121 atatacatta gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag
      181 agtaattatt gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga
      241 tgagttatat actgattatc attatccaaa aactgatgag atagtctatg gtatggatca
      301 atttatagga gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat
      361 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      421 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      481 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      541 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
//
